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Results 651 - 700 of 1091
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Carson C W - - 1994
OBJECTIVE: To investigate the hypothesis that soluble E-selectin (sE-selectin) may be detected in synovial fluid (SF) and play a role in inflammatory arthritis. METHODS: We used a sandwich ELISA to measure sE-selectin in the SF of 58 patients with rheumatoid arthritis (RA), 9 with psoriatic arthritis (PsA), 30 with osteoarthritis ...
Van Amstel S R - - 1994
Studies to evaluate changes in blood clotting, blood calcium and protein, the haematocrit and white-cell counts were undertaken in seven sheep with experimentally induced heartwater. A marked decline in thrombocyte count was recorded during the acute stage of the disease. This was associated with increases in both prothrombin time (PT) ...
Holst H - - 1994
The effect of oral intake of endotoxin (ET) combined with inhalation of ET-contaminated feed-dust was studied in 6 prepubertal gilts. The animals were given 100 mg of ET each in their ordinary feed ration and meanwhile stayed for 4 h in a continuous flow exposure chamber where the endotoxin concentration ...
Gutfilen B - - 1994
Several radionuclides and different methods have been employed as cellular labels to study inflammatory sites in man. Here we present the results obtained with white blood cells (WBC) labeled with 99mTc using a simple and low cost new technique (SnTec). WBC were incubated with 12 micrograms/ml of stannous chloride for ...
Lofsness K G - - 1994
An automated reticulocyte procedure using a flow cytometer and the fluorescent dye thiazole orange was evaluated for clinical use. The mean reticulocyte count on 118 hematologically healthy adults was 1.56% (standard deviation [SD] 0.54), with virtually no difference in percentage between women and men. The mean absolute values were 68.4 ...
Osta R - - 1994
The effect of 40 genetic markers (blood groups and biochemical polymorphisms) on 5 haematological parameters (haematocrit, haemoglobin concentration, red blood cells count, white blood cells count and differential leucocyte count) was studied in 179 fighting bulls to investigate whether genetic background was related to stress endurance. Analysis of data was ...
Deneys V - - 1994
Reduction of leucocytes in blood components, achieved by filtration, may reduce the risk of HLA alloimmunization, virus transmission, and febrile reactions. Nevertheless, white blood cell (WBC) concentrations obtained in this way can be too low to be accurately counted with automated and manual techniques. We describe here a rapid, reliable, ...
Nikodémusz E - - 1994
Variations in milk somatic cell count (SCC) and haematologic values were studied in a dairy cow colony of the Holstein-Friesian and Hungarian Red-Spotted breeds (n = 23) from May 1992 to July 1993. Milk and blood samples were taken approximately at monthly intervals and data were assigned into ten lunar ...
Chatta G S - - 1994
OBJECTIVES: Measurement of blood neutrophil (PMN) counts after the administration of hydrocortisone, granulocyte colony-stimulating factor (G-CSF) and epinephrine. DESIGN: Prospective study, with subjects serving as their own controls before and after administration of hydrocortisone, G-CSF, and epinephrine. SETTING: The G-CSF and hydrocortisone studies were conducted at the Clinical Research Center, ...
Deméocq F - - 1994
Peripheral blood stem cells (PBSCs) were collected for autotransplantation in 20 children (median age 4 years, range 0.5-10 years) weighing < 25 kg (median 14.5 kg, range 6.8-24 kg) with various malignant diseases: leukemias and lymphomas (n = 6), solid tumours (n = 14). Cytaphereses were carried out after standard ...
Keskil S - - 1994
A prospective analysis of 153 cases of head trauma was conducted with respect to their white blood cell counts. A white blood cell count exceeding 20 x 10(6)/l is associated with a poor clinical grade on admission and with a 96% mortality as opposed to that of a 23% mortality ...
Goodrick M J - - 1993
Automated blood counts from a patient with Waldenström's macroglobulinaemia repeatedly failed critical limit standards set for mean cell haemoglobin concentration and mean cell haemoglobin. Haemoglobin estimation was higher than that suggested by clinical examination, symptoms, and the spun haematocrit. This was found to be due to an interaction between the ...
Bochev B G - - 1993
Luminol chemiluminescence (LCL) of whole blood as response to zymosan stimulation was used to assess the activity of polymorpho-nuclear leukocytes (PMNLs). Since PMNL and red blood cells (RBCs) are the primary components determining LCL response, the influence of PMNL and RBC count on the response was investigated over wide ranges ...
Miller R H - - 1993
Procedures were developed to count neutrophils in milk, using a flow cytometer. Milk samples from 2 experiments were counted: 1 with 4 noninfected cows and a second with 5 noninfected cows that were injected with endotoxin in 2 mammary quarters. Thus, the procedures were evaluated on normal milk and on ...
Kristal-Boneh E - - 1993
Seasonal changes in blood count (haematocrit and haemoglobin values) have been described in studies of small samples. Since Israel is characterized by high summer temperatures, we attempted to determine whether there are circannual changes in blood count, and how they are influenced by mean monthly temperature. In a cross-sectional study, ...
Bao W - - 1993
BACKGROUND: Noting the distribution of blood values in a biracial southern community according to age, sex, and race variations will help in understanding the normative developmental changes in early life and provides background information. METHODS: Complete blood counts were obtained from 3,018 free-living children ages 5-17 years from a well-defined ...
- - 1993
Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood ...
Sothern R B - - 1993
Forty-two hematological and biochemical variables routinely measured in dogs as part of a preoperative protocol have been analyzed for circannual changes by analysis of variance (ANOVA) and single cosinor procedures. Data were available from up to 489 adult mongrel dogs of both sexes studied on weekdays over a 5-year span ...
Criss V R - - 1993
A linear peristaltic infusion device was evaluated for red cell (RBC) transfusion in the pediatric and neonatal setting. CPDA-1 RBC units (n = 24) divided into six groups of 4 units each underwent simulated transfusion. Blood was infused by using manufacturer-provided administration sets with either a 21-gauge needle or a ...
Schwartz J - - 1993
To examine specific cellular markers of inflammation in peripheral blood (neutrophils and eosinophils) and their relationship to respiratory symptoms, we used data from the First National Health and Nutrition Examination Survey (NHANES I). Cross-sectional data were available on a random sample of 6,913 adults aged 30 to 74 years who ...
Knaus R M - - 1993
Rabbits were infused with 3.7 GBq (100 mCi) of [24Na]Na ion in a 100-ml sodium carbonate solution. Beta particles were detected using a Tennelec Counting System; background counts were 1.6 +/- 1 counts per minute (cpm). Counts for one nanoliter of blood ranged from 22 to 30 cpm. Blood volumes ...
Seppä K - - 1993
In order to find out if blood count parameters could help to differentiate between the two most common causes for nonanemic macrocytosis, alcohol abuse and vitamin B12 deficiency, we studied 18 nonanemic pernicious anemia patients and 136 alcohol abusers without deficiency of folate and with an erythrocyte mean cell volume ...
Casoni I - - 1993
Recombinant human erythropoietin (EPO), commercially available since 1988, is thought to be used by athletes in aerobic sports for the purpose of increasing oxygen transport and aerobic power. In an attempt to identify EPO administration, we have studied the peripheral blood of 20 subjects practising sports at an amateur level. ...
Sont J K - - 1993
In patients with asthma there is only a weak relationship between airway responsiveness to hypertonic saline and methacholine. We tested the hypothesis that airway responsiveness to hypertonic saline in asthma is related to the presence and activity of inflammatory cells in the peripheral blood. Nineteen atopic asthmatic adults (19-28 yr; ...
Cody R P - - 1993
Hematologic surveillance data from 1940 to 1975 were analyzed for a benzene-exposed cohort of 459 rubber workers. The present analyses are restricted to 161 workers with "preemployment" counts done before exposure and rely on their subsequent counts from the first 12 months of employment. While blood cell counts declined approximately ...
Hasan R - - 1993
The authors retrospectively reviewed complete blood counts in the medical records of all babies admitted to a normal newborn nursery from January through December 1989. The subjects consisted of 326 babies who were delivered vaginally (VgD) and 138 who were delivered by Cesarean section (CS). All blood samples were drawn ...
Treleaven J - - 1993
Manufacture of an Ayurvedic arsenic-containing compound is described, which is currently in use in India to control blood counts of patients with haematological malignancies. The efficacy and side effects of this compound are evaluated in the light of the fact that arsenic was recognised to be of use in the ...
Phillips S J - - 1993
A small wave propagation pump (WPP) was incorporated into oxygenators to create a disposable heart-lung machine. The WPP provides undirectional micropulsatile laminar flow of 8 L/min against 800 mmHg. The smaller WPP can generate 4 L/min against 400 mmHg (cardioplegia), or reversed, and can create up to 150 mmHg negative ...
Morell V - - 1993
Traumatized patients frequently have leukocytosis, hypokalemia, and acidosis. The purpose of this study was to determine whether the admission serum potassium level (K+), white blood cell count (WBC), and arterial pH predicted the severity of injury in trauma patients. The injury severity score (ISS), total length of stay in the ...
Fossum T W - - 1993
Case records of 9 dogs and 5 cats with eosinophilic effusions were reviewed. The animals ranged from 11 months to 13 years old. Seven animals had pleural effusions, 5 had peritoneal effusions, and 2 had pleural and peritoneal effusions. Neoplasia was confirmed in 6 animals and suspected in 1. Eosinophilic ...
Vachula M - - 1993
Reduction of white cells (WBCs) in blood components may reduce the risk of virus transmission and HLA alloimmunization. Filtration provides a means by which to achieve high-efficiency WBC reduction. A method has been developed using flow cytometry to quantitate the number of WBCs in WBC-reduced packed red cells or platelet ...
Thomas S R - - 1993
Bone marrow depression following 131I therapy for metastatic thyroid cancer can occur in up to one-quarter of all patients so treated. An analysis was made of the 131I whole body (WB) retention and its relationship to activity in blood for 46 patients (45 adult, 1 adolescent in 49 total studies) ...
Hed J - - 1993
The FOG method is a new cell membrane permeabilization technique which makes it possible to identify the eosinophils in unseparated peripheral blood and allows analyses of both surface and intracellular antigens by flow cytometry. This technique has been adapted to analyse eosinophil activity in peripheral blood from asthmatic patients by ...
Cantinieaux B - - 1993
Accurate measurements of bacteria concentrations are required in numerous studies; they raise methodological problems that complicate, for instance, the investigations of polymorphonuclear neutrophil functions. We propose a new flow cytometric method of determining bacteria concentrations by comparison with a standardized fluorescent latex bead solution. Relative counts of beads and bacteria ...
Van Petegem M - - 1993
Flow cytometric determination of reticulocytosis is progressively replacing the manual microscopic method. We evaluated three flow cytometers (Becton Dickinson FACScan, Coulter EPICS Profile II, Ortho Cytoron Absolute) for reticulocyte enumeration, using thiazole orange. For each sample, 30,000 cells were analysed. In order to comparatively evaluate the three instruments, reticulocytes were ...
Schwartz J - - 1993
The relationship of peripheral blood leukocyte count to respiratory symptoms was explored in data from the Second Annual National Health and Nutrition Survey (NHANES II). The study sample consisted of 9237 white and nonwhite US adults between the ages of 30 and 74 years. Three respiratory symptom outcomes were utilized: ...
Paterakis G S - - 1993
We performed precise reticulocyte counts and a reticulocyte maturation study according to ribosomal RNA (rRNA) content, in normal cord blood. For this purpose we analyzed 35 cord blood specimens corresponding to a mean gestational age of 39.2 weeks (range 38-41). In all specimens complete blood counts were performed with the ...
Bridges A B - - 1992
The fibrinolytic activity of blood has a circadian variation with increased thrombotic tendency in the morning. This may be a contributory factor to the circadian variation in the time of onset of thrombotic events. However, there has recently been increasing interest to the role of the white blood cells (WBC) ...
Hayakawa T - - 1992
A simple in vivo bioassay suitable for routine testing of quality control of recombinant human erythropoietin (rHuEPO) analogues was developed. The assay took four days, normal mice were used and radioactive compounds were not needed. EPO activity was measured by the increased number of some part of reticulocytes which increased ...
Malark J A - - 1992
Peritoneal fluid and blood was collected from 8 healthy adult horses. Four 1-ml aliquots of peritoneal fluid from each horse were then contaminated with 0 ml (normal), 0.05 ml (1 drop), 0.10 ml (2 drops), and 0.20 ml (4 drops) of blood from the same horse. Samples were analyzed for ...
Buddle B M - - 1992
The dynamics of blood eosinophilia was studied in Romney lambs dosed twice weekly with 5000 infective larvae of Trichostrongylus colubriformis. A marked rise in blood eosinophil counts of the dosed lambs coincided with the start of the decline of faecal egg counts (FEC). Eosinophilia was also studied in grazing Romney ...
Reagan W J - - 1992
Hemolytic anemia was induced in five Domestic Shorthair cats (four adult males and one spayed female obtained from a breeding colony at Colorado State University, CO), and blood samples were analyzed from five other cats (two castrated male Domestic Shorthairs, one castrated male Domestic Longhair, one castrated male Persian, and ...
Rutland M D - - 1992
The splenic uptake rate of Tc-sulphur colloid or Tc-tin colloid was measured and found to correlate well with splenic function. The normal tracer uptake rate was 0.0002/s-0.0006/s (measured uptake rate divided by measured injected activity). Lower values indicated hyposplenism (sensitivity = 0.97, specificity = 0.95), and values over 0.0006/s indicated ...
Salvati A M - - 1992
BACKGROUND: An Italian EQA scheme for haemocytometry, organized by the Istituto Superiore di Sanit?, has been active for about five years (1984-1989). The aims of this programme were to evaluate the state of the art and to introduce in Italy a scheme recommended by ICSH. N. 126 public laboratories from ...
da Silva R G - - 1992
Thirty-two Polwarth sheep of ages up to 1 year were observed under temperatures varying from 10.5 to 46.5 degrees C. The following blood cell counts were made: erythrocyte (RBC), leucocyte (WBC), eosinophil (EOS), neutrophil (NEU), lymphocyte (LYM) and monocyte (MON). Other traits measured were: haemoglobin (HB), haematocrit (HT), blood glucose ...
Fukushima R - - 1992
Bacterial translocation and related mortality rates were examined in previously transfused BALB/c mice that were gavaged with 14C radioisotope-labeled Escherichia coli before inflicting a 20% full-thickness flame burn. Radionuclide counts were measured in blood obtained by retro-orbital puncture 4 hours postburn, and survival was recorded for 10 days. Radionuclide counts ...
Slavin M A - - 1992
OBJECTIVE: To compare the haematological toxicity and efficacy of oral dapsone and nebulized pentamidine as Pneumocystis carinii pneumonia (PCP) prophylaxis in HIV-infected patients receiving zidovudine. DESIGN: Randomized, prospective. SETTING: Infectious diseases hospital with participants drawn from both inpatient and outpatient departments. PATIENTS: Those eligible were starting treatment with zidovudine, needed ...
Gulati G L - - 1992
During the decade of the 1980s, a wealth of information accumulated concerning automation in hematology. Recent technological advances led the way to the development of blood cell analyzers capable of performing a ten-parameter (or greater) complete blood count and five-parameter (or greater) differential leukocyte count on a small amount of ...
Guasch R - - 1992
Several flow-cytometric methods for reticulocyte enumeration in whole blood have been developed, with different degrees of practical use. Recently, a new fluorochrome, 1,3'-diethyl-4,2'-quinolylthiacyanine (corrected) (DEQTC) was proposed in a brief report, as an alternative to thiazole orange for reticulocyte counting. We have evaluated the usefulness of this fluorescent stain by ...
Sorette M P - - 1992
Studies on normal human reticulocytes have been limited by a lack of methods for effective reticulocyte enrichment. This study shows a convenient new approach for selective enrichment of reticulocytes from normal blood samples. We have developed a modified arabinogalactan density gradient that contains high potassium levels, approximating the internal cation ...
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