Haematological estimations and serum biochemical analyses were made on 100 samples collected from clinically healthy 'Hijin' racing camels (Camelus dromedarius) in Kuwait. The red blood cell counts, packed cell volume, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and total white blood cell counts were estimated. ...

Quality control of leukocyte-reduced packed red cell units (LRprc) produced in blood facilities must conform to regulatory criteria, which state that units may not contain more than 1 x 10(6) to 5 x 10(6) white blood cells (WBC) per unit. The post-filtration WBC content of a total of n = ...

In this project we have prospectively studied the erythropoietic activity in patients with sickle cell anaemia (SS) before and after treatment with hydroxyurea (HU). Some of the patients were enrolled in a double-blind placebo controlled trial of HU in patients with SS and others were enrolled in an open label ...

Haematological and plasma biochemical constituents can be affected during capture and handling operations in wildlife. The present study compares the blood constituents of 40 red deer (Cervus elaphus) captured using two different methods: 20 were captured by physical means, using long nets and box traps, and 20 by chemical means, ...

BACKGROUND: Both lymphocytosis and lymphopenia have been observed following trauma and each has been claimed to predict mortality. An understanding of the early temporal changes in leucocyte counts may help to explain why such discrepancies have been found. The purpose of this study was to determine the early serial changes ...

OBJECTIVES: The aim of the study was to look for a rise in reticulocyte levels in workers exposed to various solvents, in comparison to non exposed control subjects. METHODS: A cohort of exposed workers was selected on the criterion of exposure to solvents, among employees regularly attending the Centre of ...

We report the novel occurrence of an erroneous automated total and differential white blood cell count on a blood sample obtained by traumatic femoral venepuncture, caused by contamination of the blood sample with subcutaneous adipose tissue. The erroneous counts were observed on a Bayer-Technicon H2 analyser while counts on a ...

Dendritic cells (DC) originate from a bone marrow (BM) precursor and circulate via the blood to most body tissues where they fulfill a role in antigen surveillance. Little is known about DC numbers in disease, although the reported increase in tissue DC turnover due to inflammatory stimuli suggests that blood ...

In nine healthy young patients, operated on for thoracic scoliosis, a pulmonary artery catheter was inserted for the study of haemodynamic variables and blood sampling during autologous transfusion of postoperatively drained blood. At 1-3 h after wound closure, 10 ml kg/body weight of drained untreated blood from the wound was ...

In this study, we determined normal values for some haematological parameters in male Sierra Leoneans--Haemaglobin concentration (HB), Packed Cell Volume (PCV), White Blood Cell Count (WBC), Red Blood Cell Count (RCC) and Erythrocyte Sedimentation rate (ESR). The result obtained differ significantly from those found in European text books as normal ...

Quality control (QC) has been introduced in laboratories, and QC surveys in leukocyte differential count to enhance quality have been performed by College of American Pathologists, Japanese Association of Medical Technologists, Osaka Medical Association and manufacturers. The results of QC survey in a manual leukocyte differential count indicated problems on ...

A good laboratory practice is the heart of clinical laboratory quality. One must establish a standard system in order to achieve the quality. However, standard system is not only the technical but also a state of the art. The socioeconomic and culture are the influence factors. At present, technique of ...

The responses of ten isolates of Enterobacteriaceae to ceftibuten exposure were monitored by measuring several parameters. Post-antibiotic effect (PAE), control-related effective regrowth time (CERT) and post-antibiotic sub-MIC effect (PA-SME) were determined by bacterial enumeration carried out either by impedance in combination with viable counting (IMP/VC) or by impedance in combination ...

The newly developed XE-2100 haematology analyser can provide complete blood counts, leukocyte differentials, perform reticulocyte analysis, and obtain quantitative data on nucleated red blood cells (NRBCs). In this study, we evaluated the basic performance of this instrument using routinely obtained blood specimens treated with ethylenediaminetetraacetic acid-2k. Reproducibility, carryover, stability during ...

A flow cytometric method to detect and study human eosinophils in whole blood was established. Normal subjects and patients with various types of eosinophilia (hypereosinophilic syndromes, allergic diseases, dermatitis, Hodgkin's Disease, parasitosis) were studied. Whole blood samples were treated for 10 minutes at room temperature with a commercially available reagent ...

From 1993 to 1995, approximately 10% of the clinically healthy northern elephant seals (Mirounga angustirostris) at The Marine Mammal Center in California exhibited a large unexplained increase in their white blood cell (WBC) count. In these animals, WBC counts ranged from 28,780 to 125,000/mm3, with a mean of 50,087/mm3. Significant ...

The purpose of this research was to determine the influence of dental amalgam on the blood count of the holder of amalgam fillings. The experiments were performed on 30 Wistar rats. One group of rats received 8 mg of fragmented dental amalgam daily incorporated in their food during 60 days ...

Cocaine is a potent vasoconstrictor that has been shown to alter hemoglobin, hematocrit, and red blood cell counts in both animals and humans. The present study evaluated whether cocaine administration induces splenic constriction in men and whether spleen-volume changes temporally correlate with altered hematologic parameters. Spleen volume was assessed at ...

BACKGROUND: The antipsoriatic drugs cyclosporin A (CyA) and etretinate have been found to influence proinflammatory eosinophilic leukocytes and pruritus. AIM: We compared the number of blood eosinophils, concentration of serum eosinophil cationic protein (ECP), and pruritus in patients with psoriasis treated with either CyA or etretinate. STUDY DESIGN: Patients with ...

Cryoglobulins may interfere with automated cell counting when proteins precipitate or blood cells aggregate. This results in spurious platelet or leukocyte counts, false values for red blood cell parameters and abnormal scattergrams. Usually it can be reversed by warming blood specimens to 37 degrees C. We describe the case of ...

Propofol and pentobarbital were used for deep sedation during prolonged mechanical ventilation (3 weeks) and nutritional supplementation in 17 clinically normal dogs in an intensive care setting. Tolerance developed to both drugs. Propofol, in combination with pentobarbital, at an infusion rate of 75 micrograms/kg of body weight per minute was ...

In order to optimize peripheral blood stem cell (PBSC) collection for transplantation, absolute CD34 counts are necessary to determine the exact time-point for sufficient leukapheresis. In an effort to establish and to validate a rapid and reproducible assay for PBSC enumeration, different recommendations for selection of monoclonal antibodies, lysis techniques, ...

A study was carried out from 1994 to 1996 to compare the resistance to naturally acquired gastrointestinal (GI) nematode infections (predominantly Haemonchus contortus) of 88 Galla and 114 Small East African (SEA) goats (does) in the sub-humid coastal region of Kenya. Live weights (LWT), blood packed cell volume (PCV) and ...

A new hollow fiber membrane oxygenator, the Medtronic Maxima Forté, was tested for gas transfer, blood path resistance and blood handling characteristics in a standardized setting with surviving animals. Three calves (mean body weight: 71 +/- 9.6 kg) were placed on cardiopulmonary bypass at a mean flow rate of 50 ...

OBJECTIVE: This study was designed to determine whether gamma cameras can be substituted for well-type scintillation counters in measuring blood radioactivity counts to be used as an input function for the quantitative measurement of cerebral blood flow (CBF). METHODS: Twelve different aqueous 123I solutions were prepared by serial dilution of ...

A study on blood cell damage after irradiation of fresh whole blood with 630 nm laser light was carried out in vitro. Various fluence rates of laser light were used with and without cooling of blood. Damage to the blood was assessed by blood cell counts, osmotic fragility measurements and ...

BACKGROUND: The influence of smoking and of nicotine substitution on the counts of total blood leucocytes and leucocyte subsets and the relations between the counts and lung function was investigated. METHODS: The study was a combined cross sectional and prospective study of 298 smokers and 136 non-smokers. Forced expiratory volume ...

This article evaluates recent research results comparing two different methods for obtaining complete blood cell counts and chemistry profiles from central venous catheters. Complete blood cell count and chemistry profile samples were obtained from 25 patients at three hospital-based infusion clinics. The results indicate no appreciable difference between these two ...

We present an electronic scheme that enables us to use a photon-counting device (photomultiplier or avalanche photodetector) for measuring extremely weak signals in a flow cytometer. It can be used as a sole detector, or in combination with other (conventional) detectors using the data acquisition hardware of a conventional flow ...

The objective of the present study was to evaluate the relationship between the pharmacokinetic parameters of pirarubicin and of its metabolite doxorubicin measured in plasma and whole blood, and the hematologic toxicity of this drug, in order to evaluate the predictability of changes in white blood cells (WBC) by single ...

During storage of whole blood samples the results of white blood cell counts on a Sysmex NE-8000 Hematology Analyser showed an apparent shift from the granulocytes towards monocytes. The effects of various storage conditions were investigated by incubating blood samples at different temperatures and time intervals. Granulocyte activation and degranulation ...

BACKGROUND: Peripheral blood eosinophilia is a particularly early and specific marker of both renal and hepatic allograft rejection. Therefore we evaluated the relationship between blood eosinophil counts and cardiac and pulmonary allograft rejection. METHODS: Differential blood counts were available within 3 days before 383 endomyocardial biopsy specimens in 56 heart ...

Fifty-two patients with solid tumors had depressed white blood cell and neutrophil counts because of prior treatment with cytotoxic cancer chemotherapeutic drugs. These patients were given one or more i.v. infusions of 0.125-0.25 mg of 12-O-tetradecanoylphorbol-13-acetate (TPA), and this treatment increased the low white blood cell and neutrophil counts toward ...

In concert with the International Society of Hematotherapy and Graft Engineering (ISHAGE), we previously described a set of guidelines for detection of CD34+ cells based on a four-parameter flow cytometry method (CD45 FITC/CD34 PE staining, side and forward angle light scatter). With this procedure, an absolute CD34+ count is generated ...

The ECAT EXACT3D (CTI/Siemens 966) 3D-only PET tomograph has unprecedented sensitivity due to the large BGO (bismuth germanate) detector volume. However, the consequences of a large (23.4 cm) axial field-of-view (FOV) and the need for a patient port diameter to accommodate body scanning make the device more sensitive to photons ...

The objective of this study was to determine the effect of intravascular intrauterine transfusion (IUT) on fetal leukocyte counts and subsets. For this purpose, pre- and post-transfusion blood samples of 81 fetuses, receiving a total of 253 IUTs, were compared. Immediately after the IUT procedure an average decrease in fetal ...

We studied white blood cell (WBC) count and erythrocyte sedimentation rate (ESR) in 241 consecutive patients with acute ischemic stroke. White cell count was elevated in 20% of the patients, ESR was elevated in 68%. Each increase significantly correlated with outcome, as evaluated by either lesion size at CT scan ...

This study evaluated the Pico-Count (Bioscan, Inc., Washington, DC) flow-through radioactivity detector, designed for use in PET studies of cerebral blood flow. METHODS: The Pico-Count detects the two 511-keV positron annihilation photons with two bismuth germanate detectors operating in coincidence. The detectors, photomultipliers and preamps are housed within a 12 ...

The effects of short-term physical restraint and isoflurane anesthesia on hematologic and serum biochemistry parameters were evaluated in 12 island flying foxes (Pteropus hypomelanus). Physical restraint was associated with significantly decreased calcium, cholesterol, globulin, albumin, hemoglobin concentrations, red blood cell count, and hematocrit and increased glucose, phosphorus, and potassium concentrations. ...

A prospective ecopathogical survey was conducted in French commercial dairy herds located in Brittany. Previous production and reproduction data and blood parameters were used to identify predictive indicators of risk for retained placenta (RP) in Black-Pied cows. All the cows had delivered a single calf after a dry period of ...

Peripheral blood progenitor cells (PBPC) reside within the mononuclear cell (MNC) component of the blood and can be collected using a number of apheresis devices, including the Fenwal CS3000 Plus Blood Cell Separator. Increased MNC collection efficiency, therefore, may reduce the number of apheresis required to achieve collection goals. In ...

The effect of hypergravity on the white blood cell (WBC) line of mice was investigated by use of horizontal centrifuge. Several sets of experiments were performed, in which the parameters measured were the WBC and differential cell count in the peripheral blood. In another experiment, lymphocyte counts from the spleen, ...

The analytic precision of an automated blood analyzer, the Technicon H*1(R), was evaluated utilizing blood samples collected from 20 piglets at 1 and 14 days of age. The effect of storing the blood samples at 4 degrees C for 24 and 48 hours also was determined. Blood samples were analyzed ...

Complete blood counts (CBC) of umbilical cord blood from 123 healthy term newborns were simultaneously performed with two different cytometers using laser as a light source. Medians (95% range) were: WBC 14.2 (7.8-24.3) x 10(9)/l, platelets 265 (174-363) x 10(9)/l, Hb 15.7 (12.5-18.2) g/dl, RBC 4.6 (3.9-5.5) x 10(12)/l, MCV ...

The aim of this study was to investigate the predictive value of peripheral eosinophil and monocyte blood counts regarding lung function in smokers and non-smokers, and to investigate the influence of smoking on these cell counts. Forced expiratory volume in 1 s (FEV1) measurements and blood samples were collected from ...

OBJECTIVE: Laboratory studies have documented a wide range of pesticide-induced changes in the hematopoietic and lymphoreticular systems. Some of these are expressed as altered serum values, blood cell counts, and leucocyte functions. The goal of the present study was to determine whether these alterations were evident in peripheral blood of ...

Changes in haematological parameters were determined in West African Dwarf goats subjected to a 30% haemorrhage. Analyses were made before and at the end of bleeding and at 1, 4 and 24 h after haemorrhage. Significant decreases in the red blood cell count, packed cell volume and haemoglobin concentration, which ...

Although EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is of practical importance because failure to recognize this clinical entity may result in misdiagnosis and subsequent mismanagement of the patients, the pathophysiological nature of EDTA-PTCP remains unknown. To develop an effective way to evaluate the platelet counts in patients with EDTA-PTCP, we introduced aminoglycosides-supplemented anticoagulating ...

Twelve free-ranging mouflon (Ovis ammon), eight adults and four lambs, were captured in northeast Spain. The animals were transported for 9 hr from the capture site to the Wildlife Rescue Center at Vallcalent (Lleida), 300 km SW. Blood samples were taken at capture and after transport to study the effects ...

Leukodepleted or leukocyte-poor blood products (fresh-frozen plasma, packed red cell and platelet concentrates in particular) are widely used in current clinical practice. However, because the monitoring of leukodepletion efficiency is generally carried out (if at all) using the labour-intensive and relatively inaccurate manual Nageotte chamber technique, it is clear that ...