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Bai Zhouxian Z *CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences †CAS Key Laboratory of Genome Sciences and Information, University of Chinese Academy of Sciences, Beijing, China ‡Department of Neurology and MIND Institute, School of Medicine ∥Alzheimer's Disease Center, School of Medicine, University of California at Davis, Sacramento, CA §Department of Medicine, University of Maryland School of Medicine, Baltimore, - - 2014
Defining the RNA transcriptome in Alzheimer Disease (AD) will help understand the disease mechanisms and provide biomarkers. Though the AD blood transcriptome has been studied, effects of white matter hyperintensities (WMH) were not considered. This study investigated the AD blood transcriptome and accounted for WMH. RNA from whole blood was ...
Najafabadi Zahra Ghayour ZG Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, - - 2014
This study investigated loop-mediated isothermal amplification (LAMP) detection of Plasmodium falciparum and Plasmodium vivax in urine and saliva of malaria patients. From May to November 2011, 108 febrile patients referred to health centers in Sistan and Baluchestan Province of south-eastern Iran participated in the study. Saliva, urine, and blood samples ...
Paris Sandra S French Blood Establishment Rhône Alpes, Lyon, - - 2014
The poor suitability of standard hemagglutination-based assay techniques for large-scale automated screening of red blood cell antigens severely limits the ability of blood banks to supply extensively phenotype-matched blood. With better understanding of the molecular basis of blood antigens, it is now possible to predict blood group phenotype by identifying ...
Alsalhi M S - - 2014
Thalassemias (Thal) are forms of inherited autosomal recessive blood disorders arising out of mutations in the chromosomes 11 or 16. These disorders lead to poor oxygen delivery to blood vessels and consequent splenomegaly, bone deformities, and shorter life spans. The most common detection methods for Thal are complete blood count ...
Umbria Miriam M Unitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Facultat de Biocièncias, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, - - 2014
The main goal of this study is to increase knowledge on the molecular level of the ABO blood group system in Europe by providing data for Poland, Spain, and Andorra populations. A total of 172 oral scrapings samples from individuals of Polish origin, 108 peripheral blood samples of autochthonous individuals ...
Torrance Hew Dt HD *Adult Critical Care Unit, Royal London Hospital, Barts Health NHS Trust, London †Centre for Trauma Sciences, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London ‡Centre for Translational Medicine and Therapeutics, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London §Genome Centre, John Vane Science Centre, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, - - 2014
To explore the hypothesis that blood transfusion contributes to an immunosuppressed phenotype in severely injured patients. Despite trauma patients using disproportionately large quantities of blood and blood products, the immunomodulatory effects of blood transfusion in this group are inadequately described. A total of 112 ventilated polytrauma patients were recruited. Messenger ...
Alvarez-Dominguez Juan R JR Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, - - 2014
Long noncoding RNAs (lncRNAs) are increasingly recognized to contribute to cellular development via diverse mechanisms during both health and disease. Here, we highlight recent progress on the study of lncRNAs that function in the development of blood cells. We emphasize lncRNAs that regulate blood cell fates through epigenetic control of ...
Evans Cory J CJ Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, - - 2014
Analyses of the Drosophila hematopoietic system are becoming more and more prevalent as developmental and functional parallels with vertebrate blood cells become more evident. Investigative work on the fly blood system has, out of necessity, led to the identification of new molecular markers for blood cell types and lineages and ...
Tilley Louise L International Blood Group Reference Laboratory, NHS Blood and Transplant, Bristol, UK. Electronic address: - - 2014
Blood group genotyping has many advantages over conventional phenotyping for both blood donors and patients, and a number of high-throughput methods have now been developed. However, these are limited by a requirement for existing knowledge of the relevant blood group gene polymorphisms, and rare or novel mutations will not be ...
St-Louis Maryse M Recherche et développement, Héma-Québec, Québec, Québec, Canada. Electronic address: - - 2014
For many decades, hemagglutination has been the sole means to type blood donors. Since the first blood group gene cloning in the early 1990s, knowledge on the molecular basis of most red blood cell, platelet and neutrophil antigens brought the possibility of using nucleotide-based techniques to predict phenotype. This review ...
Miri-Moghaddam Ebrahim E Genetics of Non-Communicable Disease Research Centre, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Genetics, Zahedan University of Medical Sciences, Zahedan, Iran. Electronic address: - - 2014
To determine the distribution of Duffy blood group genotypes in Balouch population as a major ethnic group that living in a sub-tropical area in south East of Iran. In this study, the Duffy blood group FY phenotypes were determined using indirect anti-globulin technique and also genotype by PCR-RFLP in 160 ...
Haklová B B Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak - - 2014
SUMMARY The blood parasites from the genus Hepatozoon Miller, 1908 (Apicomplexa: Adeleida: Hepatozoidae) represent the most common intracellular protozoan parasites found in snakes. In the present study, we examined 209 individuals of snakes, from different zoogeographical regions (Africa, America, Asia and Europe), for the occurrence of blood parasites using both ...
Rehkopf David H DH Department of Medicine, Stanford University, Stanford, California, - - 2014
Peripheral blood leukocyte telomere length (LTL) is increasingly being used as a biomarker of aging, but its natural variation in human populations is not well understood. Several other biomarkers show seasonal variation, as do several determinants of LTL. We examined whether there was monthly variation in LTL in Costa Rica, ...
Tasker S S School of Veterinary Sciences & Langford Veterinary Services, University of Bristol, Langford House, Langford, Bristol, BS40 - - 2014
The aim of this study was to determine the agreement between AB blood phenotyping and genotyping and determine whether non-AB blood type incompatibilities exist in UK cats. Blood samples underwent phenotyping (A, B or AB) using microplate agglutination, and genotyping (AA, Ab or bb) using pyrosequencing of a fragment of ...
Düvel Anna A Immunology Unit, University of Veterinary Medicine, Foundation, Hannover, Hannover, - - 2014
Subclinical endometritis (SCE) is an important postpartum disease in dairy cows, but conventional cytobrush diagnosis often gives imprecise results. The aim of this study was to analyze disease-associated changes in peripheral blood as potential diagnostic parameters. Cellular subpopulations of blood leukocytes from cows with or without SCE (45-55 days postpartum) were ...
de Paula Sabino Adriano A Faculty of Pharmacy, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil, - - 2014
Recent studies have demonstrated association between ABO blood system and thrombosis, indicating that individuals belonging to non-O blood groups (A, B or AB) present an increased risk of venous thrombosis, heart disease, and ischemic stroke (IS) as compared to O blood group carriers. In this study, we investigated the frequency ...
França Angela A CEB-IBB, Centro de Engenharia Biológica - Instituto de Biotecnologia e Bioengenharia, Universidade do Minho, Braga, Portugal; Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, - - 2014
Staphylococcus epidermidis biofilm formation on the surface of intravenous catheters is responsible for 22% of the cases of bloodstream infections, in patients in intensive care units in the USA. The ability of S. epidermidis to withstand the high bactericidal activity of human blood is therefore crucial for systemic dissemination. In ...
Macedo-Silva Virgínia P VP Department of Biochemistry, Biosciences Center, Universidade Federal do Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, Natal, RN, Brazil, - - 2014
Leishmania infantum, the causative agent of visceral leishmaniasis (VL) in Brazil, is spread mostly by the bite of the sand fly Lutzomyia longipalpis (Lutz & Neiva). We trapped sand flies in endemic neighborhoods near Natal, Brazil, where cases of human and dog VL were documented. Amplification of species-specific cytochrome b ...
Končarević Saša S Proteome Sciences R&D GmbH & Co. KG, Altenhöferallee 3, 60438 Frankfurt am Main, - - 2014
Peripheral blood mononuclear cells (PBMCs) are an easy accessible cellular part of the blood organ and, along with platelets, represent the only site of active gene expression in blood. These cells undergo immunophenotypic changes in various diseases and represent a peripheral source of monitoring gene expression and posttranslational modifications relevant ...
Binder Alexandra M - - 2013
Investigation of the biological mechanism by which folate acts to affect fetal development can inform appraisal of expected benefits and risk management. This research is ethically imperative given the ubiquity of folic acid fortified products in the US. Considering that folate is an essential component in the one-carbon metabolism pathway ...
Wehmeier Udo F - - 2013
An increasing number of studies examining gene expression associated with diseases in children is likely, in the near future, to provide simple and easy to use methods for the isolation of RNA for gene expression profiling. Prerequisites for such studies are likely to encompass the use of small amounts of ...
Rajatileka Shavanthi - - 2013
Genotyping requires biological sample collection that must be reliable, convenient and acceptable for patients and clinicians. Finding the most optimal procedure of sample collection for premature neonates who have a very limited blood volume is a particular challenge. The aim of the current study was to evaluate the use of ...
Franssen Nathan R NR Department of Biology and Museum of Southwestern Biology, University of New Mexico 167 Castetter Hall, Albuquerque, New Mexico - - 2013
Understanding population-level responses to human-induced changes to habitats can elucidate the evolutionary consequences of rapid habitat alteration. Reservoirs constructed on streams expose stream fishes to novel selective pressures in these habitats. Assessing the drivers of trait divergence facilitated by these habitats will help identify evolutionary and ecological consequences of reservoir ...
Dunham Richard M - - 2013
Persistent systemic inflammation is associated with the inability of some HIV-infected patients to normalize circulating CD4+ T-cell levels after years of suppressive antiretroviral therapy (ART). In this work, we sought to understand whether such systemic inflammation is also associated with detectable signs of inflammation in biopsies from the rectosigmoid colon. ...
Flôres Marli Aparecida Luvisuto Rossett - - 2013
Polymorphisms of Rh, Kell, Duffy, Kidd and Diego blood group systems were studied in 209 unrelated Brazilian Japanese descendants from South of Brazil. The methods used were multiplex-PCR, AS-PCR and RFLP-PCR. The differences in frequencies among the populations were evaluated using chi-square test. The frequencies for Rh, Kell, Kidd and ...
Dubey A - - 2013
Polymerase chain reaction (PCR) offers great promise for the rapid, sensitive and specific diagnosis of tuberculosis (TB). However, blood TB PCR is still not standardised. Evaluation of the Blacklight® Card to standardise TB PCR in blood samples using a multiplex nested PCR method targeting insertion sequence 6110 and MPB64 sequence ...
Tylee Daniel S - - 2013
In this article, we review studies detailing the correspondence between peripheral blood and brain tissue across various domains of high-throughput -omic analysis in order to provide a context for evaluating blood-based biomarker studies. Specifically, we reviewed seven studies comparing patterns of DNA methylation (i.e., an aspect of the epigenome), eight ...
Mohamed S S IGM Hospital, Laboratory Services, Kan'baa aisaarani hingun, Male', 20402, Maldives. - - 2013
We here report the first study on the distribution of red cell antigens and phenotype frequencies of various blood group systems in Maldives. Randomly selected 123 regular blood donors of O group were phenotyped for seven blood group systems by direct tube agglutination and or indirect antiglobulin tests. Blood group ...
Yoshiba Satoko - - 2013
The emergence of left-right (L-R) asymmetry during embryogenesis is a classic problem in developmental biology. It is only since the 1990s, however, that substantial insight into this problem has been achieved by molecular and genetic approaches. Various genes required for L-R asymmetric morphogenesis in vertebrates have now been identified, and ...
Boccoz S A - - 2013
At present, 33 blood groups representing over 300 antigens are listed by the International Society of Blood Transfusion (ISBT). Most of them result from a single nucleotide polymorphism (SNP) in the corresponding DNA sequence, i.e. approx. 200 SNPs. In immunohematology laboratories, blood group determination is classically carried out by serological ...
Sarkar R S RS Commandant, 151 Base Hospital, C/o 99 APO, - - 2013
The Rh system is the major blood group system besides ABO system. Even after proper blood grouping and cross matching there is a possibility of alloimmunization and antibody production in the recipients against the Rh or minor blood group antigens like Kell, MNSs, Duffy etc. Keeping in view the heavy ...
Santiago Jose A - - 2013
Diagnosis of Parkinson's disease (PD) currently relies on assessment of motor symptoms. Recently, sensitive, specific, and readily available splice variant-specific biomarkers were identified in peripheral blood from participants in the Diagnostic and Prognostic Biomarkers in Parkinson Disease study. Here we test for an association between candidate splice variant biomarkers and ...
Stehlíková O - - 2013
Minimal residual disease (MRD) detection has become increasingly important for the assessment of therapy response in chronic lymphocytic leukemia (CLL). However, current MRD analysis methods, both molecular genetic and flow cytometric, are time-consuming and require experienced laboratory staff. To reduce the demands of flow cytometric MRD detection in CLL, we ...
Wei Panpan - - 2013
Long non-coding RNAs (lncRNAs) are defined as a class of nonprotein-coding transcripts greater than 200 nucleotides in length, which have diverse functions in development and diseases including hematopoiesis. Recent advances have revealed that lncRNAs regulate hematopoietic development at almost every stage, including differentiation of the myelocyte, lymphocyte, and erythrocyte. Abnormal ...
Todua F - - 2013
Functional state of the red blood system is being studied in patients with adenoma and carcinoma of thyroid gland. The aim of this study was to determine the possible predictors of the individual radiosensitivity and the probability of developing cancer on the basis of the comparable characteristic of functional state ...
Haer-Wigman Lonneke - - 2013
In recent years genotyping methods have been implemented in blood banks as alternative to comprehensive serologic typing. We evaluated a newly developed assay for convenient and comprehensive genotyping of blood group alleles based on multiplex ligation-dependent probe amplification (MLPA) technology. We analyzed 103 random and 150 selected samples to validate ...
Santiago-Alarcon Diego - - 2013
SUMMARY Culicoides vectors can transmit a diverse array of parasites and are globally distributed. We studied feeding preferences and seasonal variation of Culicoides (Diptera: Ceratopogonidae) vectors in an urban forest of Germany to determine whether humans living nearby are readily exposed to vector-borne parasites from wild animals. We used a ...
Wawrocka Kamila - - 2013
Co-speciation between host-parasite species is generally thought to result in mirror-image congruent phylogenies. For the last several centuries, many bat species have been turning synanthropic, especially those that are hosted by bedbugs in Europe. There is evidence of only limited gene flow from the population of people to the population ...
Torti Lorenza - - 2013
Frequent blood loss induces progressive depletion of iron stores, leading to iron deficiency and, ultimately, to overt iron-deficient anaemia. The erythropoietin-mediated bone marrow response to anaemia is under the control of hypoxia-inducible factors (HIF), the master regulators of oxygen and iron homeostasis. Since the HIF-1α(Pro-582-Ser) variant is associated with elevated ...
Denomme Gregory A - - 2013
Blood group genotyping is gaining widespread adoption in blood centres and transfusion services. The current interest for a blood centre is its use as a screening tool to accurately predict donor phenotypes. However, not only is blood group genotyping used to screen for uncommon and rare types on a mass-scale, ...
Edinur H A - - 2013
In recent years, with the application of genotyping technology, there has been a substantial increase in the number of reported blood group alleles. This survey was designed to evaluate new molecular blood group genotyping methods and compile reference blood group data sets for Polynesian and Maori subjects. Subsequent analyses of ...
Manokhina Irina - - 2013
Homologous (or allogeneic) blood doping, in which blood is transferred from a donor into a recipient athlete, is the easiest, cheapest, and fastest way to increase red cell mass (hematocrit) and therefore the oxygen-carrying capacity of the blood. Although thought to have been rendered obsolete as a doping strategy by ...
Ségurel Laure - - 2013
The ABO histo-blood group, first discovered over a century ago, is found not only in humans but also in many other primate species, with the same genetic variants maintained for at least 20 million years. Polymorphisms in ABO have been associated with susceptibility to a large number of human diseases, ...
Kuuliala Krista - - 2013
Peripheral blood mononuclear cells of Crohn's disease (CD) patients with the common 1007fs mutation of the caspase recruitment domain-containing 15/nucleotide-binding oligomerization domain-containing 2 (CARD15/NOD2) gene show impaired nuclear factor kappa B (NF-κB) activation in response to muramyl dipeptide (MDP), as determined by Western blotting. We applied phospho-specific flow cytometry to ...
Golding Jean - - 2013
Selecting appropriate controls for studies of genetic variation in case series is important. The two major candidates involve the use of blood donors or a random sample of the population. We compare and contrast the two different populations of controls for studies of genetic variation using data from parents enrolled ...
Barcelos Gustavo Rafael Mazzaron - - 2013
Methylmercury (MeHg) toxicity may vary widely despite similar levels of exposure. This is hypothetically related to genetic differences in enzymes metabolizing MeHg. MeHg causes oxidative stress in experimental models but little is known about its effects on humans. The aims of the present study was to evaluate the effects of ...
Jazwa Agnieszka - - 2013
Gene therapy stimulating the growth of blood vessels is considered for the treatment of peripheral and myocardial ischemia. Here we aimed to achieve angiogenic synergism between vascular endothelial growth factor-A (VEGF-A, VEGF) and fibroblast growth factor 4 (FGF4) in murine normoperfused and ischemic limb muscles. Adeno-associated viral vectors (AAVs) carrying ...
Nick J A - - 2013
BACKGROUND: Acute pulmonary exacerbations accelerate pulmonary decline in cystic fibrosis (CF). There is a critical need for better predictors of treatment response. OBJECTIVE: To test whether expression of a panel of leucocyte genes directly measured from whole blood predicts reductions in sputum bacterial density. METHODS: A previously validated 10-gene peripheral ...
Nuzzolo Eugenia R - - 2013
Endothelial colony-forming cells (ECFC) are endowed with vascular regenerative ability in vivo and in vitro. In this study we compared the genotypic profile and the immunogenic potential of adult and cord blood ECFC, in order to explore the feasibility of using them as a cell therapy product. ECFC were obtained ...
Minato Yusuke Y Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, - - 2013
Na(+)/H(+) antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na(+)/H(+) antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na(+)/H(+) antiporters, NhaA and NhaB, are completely attenuated in ...
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