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Bagley L G - - 1991
Turkey eggs were incubated at high altitude (1,700 m). Hatchability, hematology, and growth data were analyzed for the following treatments: 1) eggs receiving supplemented oxygen (SO) during the 1st wk of incubation (control eggs received no SO during the 1st wk); 2) eggs receiving SO during the 4th wk (control ...
Tramacere P - - 1991
Bull's algorithm, in its "revisited" formulation, represents one of the main quality control (QC) procedures in several multichannel automatic hematological analyzers. Its efficacy, however, is reduced in the theoretical event that red blood cells (RBC) and hemoglobin (Hgb) undergo a concomitant analytical drift while mean corpuscular volume (MCV) remains unaffected, ...
Qualtieri A - - 1991
A fast-moving gamma chain variant was discovered in the cord blood of a newborn during a screening program carried out in the northern region of Calabria, Southern Italy. The structural analysis showed a Gly----Glu substitution at position 25 of the G gamma chain indicating a new fetal hemoglobin variant that ...
Snyder S R - - 1991
Several intramolecularly cross-linked hemoglobins having properties useful as blood substitutes have been developed. At least one of these, HbXL99 alpha, is amenable to large-scale production. This hemoglobin, and perhaps other cross-linked derivatives as well, is sufficiently heat stable to achieve complete viral inactivation. This makes it possible to use human ...
Rabino Massa E - - 1991
A young male from Apulia region (Southern Italy), heterozygous carrier of Hb-J Calabria (beta 64(E8) Gly-Asp), was described here. He showed mild polycythemia, and his whole blood dissociation curve for oxygen was clearly left-shifted, with a decreased p50. But these characteristics were not only due to the hyperaffinity of the ...
Mouneimne Y - - 1990
Rightward shifts of 50-100% of the P50 values in the oxygen dissociation curve of intracellular hemoglobin are obtained after encapsulation of inositol hexaphosphate in mouse and dog red blood cells (RBC) by electroporation. Life spans of mouse RBC-myo-inositol hexaphosphate in circulation are unchanged from the normal RBC values.
Sato K - - 1990
For the determination of total hemoglobin (Hb) in blood containing elevated carboxyhemoglobin (COHb), a newly developed reagent containing a 100-fold concentration of ferricyanide (20 g/l) and a 2-fold concentration of Sterox SE was compared with a standard reagent (0.2 g/l ferricyanide), the reagent of van Kampen and Zijlstra, using forensic ...
Vlahakes G J - - 1990
Recent concerns regarding the safety of the national blood supply have rekindled interest in the development of blood substitutes. Clinical studies have dampened the initial enthusiasm for fluorocarbon solutions as blood substitutes. The potential of hemoglobin solutions as blood substitutes has continued to stimulate investigations. However, the development of an ...
Gilson S D - - 1990
To evaluate the sensitivity and specificity of 2 commercial test kits for detection of occult blood in canine feces, various volumes of blood were administered to 6 dogs via orogastric tube. Blood volumes tested were chosen on the basis of hemoglobin quantities of 5, 10, 20, 200, 350, and 500 ...
Petersen A - - 1990
Absolute 31P-NMR measurements of ATP, ADP and 2,3-diphosphoglycerate (2,3-DPG) in oxygenated and partly deoxygenated human erythrocytes, compared to measurements by standard assays after acid extraction, show that ATP is only 65% NMR visible, ADP measured by NMR is unexpectedly 400% higher than the enzymatic measurement and 2,3-DPG is fully NMR ...
Honig G R - - 1990
Fifteen individuals among four generations of a family of English ancestry demonstrated elevated hemoglobin levels accompanied by leftward-shifted whole blood oxygen equilibrium curves. Five of the affected family members have required phlebotomies for relief of symptoms attributable to erythrocytosis. An abnormal hemoglobin or globin chain could not be isolated, but ...
Kim S J - - 1990
Effects of ionic (Hypaque-76) and nonionic (Isovue-370 and Omnipaque-350) contrast media on oxyhemoglobin dissociation of normal human red blood cells were evaluated. In series 1, 4-mL venous blood samples were obtained from 15 normal human volunteers. One blood sample served as control, and 1 mL of either of the three ...
Yabuki A - - 1990
A pyridoxalated hemoglobin-polyoxyethylene conjugate (PHP) is an oxygen carrier prepared from outdated human red cells through chemical modifications of hemoglobin (Hb). PHP was evaluated as a physiologic oxygen carrier. PHP was synthesized from a pyridoxalated hemoglobin (PLP-Hb) and an activated diester of polyoxyethylene (POE). The average numbers of molecules of ...
Tohgi H - - 1990
Oxygen affinity of hemoglobin, erythrocyte 2,3-diphosphoglycerate (DPG) and adenosine triphosphate (ATP) concentrations were compared before and after oral administration of vinpocetine (TCV-3B) (15 mg/d), a primarily vasodilating agent, for three weeks in eight patients with vascular dementia of the Biswanger type which is characterized by diffuse myelin pallor and multiple ...
Garver F A - - 1990
To facilitate the screening of blood for the presence of hemoglobins S or C, we devised an enzyme-linked immunoassay (ELISA). The ELISA procedure incorporated a murine monoclonal antibody (mAb), beta s-1, which recognized both Hb variants but did not react with Hb A, Hb A2 or Hb F. Hemoglobins in ...
Duck S C - - 1990
The stability of fresh and out-of-date Blood Bank standards for quality control of glycated hemoglobin testing was extensively documented. Eight blood standards were prepared as aliquots of washed erythrocytes, stored at -70 degrees C, and assayed in duplicate repeatedly for 24 to 42 months. Significant differences over time were noted ...
Mayberg M R - - 1990
A porcine model for subarachnoid hemorrhage has been developed to allow the selective application of blood and its components to cerebral arteries. Whole blood was centrifuged to produce two fractions consisting of washed erythrocytes (red blood cells, RBC's) and white blood cells (WBC) plus platelet-rich plasma (PRP); the RBC fraction ...
Spence R K - - 1990
To clarify the widespread practice of preoperative transfusion to attain a 10 g/dL level of hemoglobin, the relationship between preoperative hemoglobin level, operative blood loss, and mortality was studied by analyzing the results of 113 operations in 107 consecutive Jehovah's Witness patients who underwent major elective surgery. Ninety-three patients had ...
Hanioka T - - 1990
The hemoglobin concentration (Hb index) and oxygen saturation (apparent SO2) in human gingiva were estimated by tissue reflectance spectrophotometry (TRS). The gingiva had significantly lower Hb index and higher apparent SO2 than those in alveolar mucosa, but there was no difference in either parameter among different gingival areas. The reproducibility ...
Taber K H - - 1990
The rates at which the paramagnetic compounds deoxyhemoglobin (Hb) and methemoglobin (MHb) form in vivo within an area of hemorrhage are unknown. The present experiment establishes the baseline concentrations and rates of change in paramagnetic hemoglobin concentrations, as well as the pH in normal heparinized and clotted human blood maintained ...
Mairbäurl H - - 1990
We measured hematological and erythrocyte O2 transport parameters in whole blood and density-separated erythrocytes in 11 mountaineers before and during 5 days of exposure to high altitude (4,559 m). We determined the in vivo (arterial pHblood and PCO2) and standard (pHblood = 7.4, PCO2 = 40 Torr) O2 tension at ...
Glaister D H - - 1990
Cerebral oxygen sufficiency was studied noninvasively, using multiwavelength near-infrared spectrophotometry, in eight subjects exposed to lower body negative pressure (LBNP) of up to -90 mm Hg to induce presyncopal symptoms and signs. LBNP caused only small changes in the forebrain measures until the last 60 s of the exposures, whereupon ...
Schmidt W - - 1990
Hemoglobin oxygen binding properties and acid-base status were investigated in Colombian athletes (A) and controls (C) from Cali (C-1000 m) and Bogotá (B-2600 m). [Hb] and Hct values were not influenced by altitude, but Hct was lower in the blood of athletes (in Cali 2.6%, in Bogotá 1.4%). Both training ...
Vagianos C - - 1990
Six pigs were used to study whether the collection of shed blood by means of surgical compresses instead of suction traumatizes such blood. In an in vivo part of the study, the whole blood volume of the pigs was retransfused after treatment with citrated compresses, and in an in vitro ...
Gopala Krishna, G.
Ultrasonic absorption is determined in human blood and its constituents such as RBC, hemoglobin and plasma using ultrasonic interferometer technique at the frequency of 3 MHz. The study suggests that when an acoustic wave passing through a blood sample it encounters three different media namely plasma, cell wall and hemoglobin. ...
Fogh-Andersen N - - 1990
An easy method to measure blood volume is clinically needed. We used carbon monoxide (CO) and the OSM3 to measure circulating hemoglobin and blood volume with the indicator dilution principle. 50 mL of CO was administered into a closed rebreathing system and taken up via the lungs, and the amount ...
Wimberley P D - - 1990
Two methods for the routine determination of blood hemoglobin oxygen affinity are described. Both methods use whole blood and do not require special equipment, tonometry or special gas mixtures. The first method consists of a one-point determination of p50, and requires only 200 microL to 400 microL of whole blood, ...
Hellwig U - - 1990
Desferrioxamine (DFO) in therapeutically useful amounts was incorporated into red blood cells (RBC) by means of a dialysis method. Entrapped DFO interacted with hemoglobin which was indicated by the formation of methemoglobin. This process was influenced to a great extent by the duration and temperature of the incubation steps. In ...
Zwart A - - 1990
Spectral differences between hemoglobin derivatives from adult-(HbA) and fetal-hemoglobin (HbF) make the accurate determination of carboxyhemoglobin (COHb) troublesome in blood of newborns. Only the newest dedicated instruments for the determination of COHb take these differences into account. However, since there are virtually no spectral differences between both hemoglobins in the ...
Nagai M - - 1990
Absorption spectra of erythrocyte hemolysate from a normal subject, the Hemoglobin (Hb1) M Saskatoon patient and the Hb M Boston patients were investigated. Difference spectrum between the hemolysate from either freshly obtained or 37 degrees C-incubated blood of the Hb M Saskatoon patient and that of the normal blood suggested ...
Labotka R J - - 1990
Hb Mizuho [beta 68(E12)Leu----Pro] was identified in a child of Italian/Sicilian descent who exhibited severe, transfusion dependent hemolytic anemia which improved following splenectomy. The patient's peripheral blood smear, which prior to splenectomy demonstrated coarse erythrocytic basophilic stippling, showed large, dense erythrocytic hemoglobin inclusions following splenectomy. Whole blood oxygen equilibrium results ...
Rabinovici R - - 1989
To improve the outcome of trauma victims and of patients undergoing high-blood-loss surgical procedures and to avoid the many serious complications of blood transfusion, there is a need for an oxygen-carrying blood substitute. Synthetic erythrocytes composed of liposome-encapsulated hemoglobin (LEH) represent one of the significant research efforts in this direction. ...
Gibbons I - - 1989
We describe an immunoassay system suited to patient-side assay of therapeutic drugs and blood proteins. The system consists of an electronic monitor and single-use plastic cartridges containing dry reagents and liquid diluents. The monitor is turned on by insertion of a cartridge. To run the test, the user applies an ...
Lundsgaard-Hansen P - - 1989
The different versions of autologous blood transfusion have rekindled interest in a generally valid 'minimum acceptable hemoglobin concentration' of patients around or below 10 g Hb/dl. The adequate Hb concentration capable of covering the oxygen demands of the body depends on several variables measurable at the bedside: oxygen consumption VO2, ...
Bartels P C - - 1989
Direct measurement of the zinc protoporphyrin:haemoglobin ratio (ZPP:Hb ratio) in blood samples is performed by using a haematofluorimeter. Interference by non-specific fluorophores can be eliminated by removing the plasma and making the measurement on washed red blood cells (RBCs). After re-suspending RBCs in isotonic saline, haematofluorimeter readings for the ZPP:Hb ...
Mairbäurl H - - 1989
Oxygen transport by erythrocytes was studied in eight patients on maintenance hemodialysis before, during and after a 2-week stay at an altitude of 2000 m. Dialysis was continued at that altitude. In all tests, blood samples were collected one or two days following hemodialysis. Pre-altitude tests: The patients exhibited anemia ...
Linnik M D - - 1989
Electrically stimulated neurogenic vasodilation and endothelial-dependent cholinergic vasodilation in cerebral arteries are both blocked by hemoglobin. To determine if neurogenic vasodilation has a cholinergic component, we examined the effect of hemoglobin on neurogenic responses and perivascular cholinergic parameters in isolated porcine cerebral arteries. The perfused circle of Willis has a ...
Garry F - - 1989
Examination of the diseased llama often relies on clinical laboratory information to supplement that from the physical examination and history. Because of the llama's only recent importance as a companion animal, little information is available on the analysis and interpretation of clinical laboratory parameters in this species. Llamas possess red ...
Kutlar F F Department of Cell and Molecular Biology, Medical College of Georgia, Augusta - - 1989
We introduce a new high-performance liquid chromatographic procedure that uses a specific anion-exchange column for the separation of hemoglobin (Hb) Bart's (gamma 4), Hb H (beta 4), Hb Portland-I (zeta 2 gamma 2), Hb Portland-II (zeta 2 beta 2), and the abnormal Hb Constant Spring (alpha 2 extended beta 2) ...
Jopski B - - 1989
Hemoglobin (Hb) was encapsulated in phosphatidylcholine vesicles by removal of the detergent n-octyl beta-D-glucoside (OG) or n-octyltetraoxyethylene (C8E4) out of mixed detergent-lipid micelles in Hb solution. Three types of apparatus were used for dialysis. Dialysis buffer flow rates, the surface area of the dialysis membranes, and detergent-lipid interactions determined the ...
Westgren M - - 1989
The feto-placental blood volume was studied using a hemoglobin hemodilution technique in 15 erythroblastotic fetuses at 43 intravascular transfusions. Four severely anemic fetuses (Hb 30 g/l) had significantly larger blood volumes than 11 moderately anemic fetuses (Hb 30-79 g/l), 169 +/- 37 versus 105 +/- 32 ml/kg fetal body weight ...
Vidal-Naquet A - - 1989
Encapsulation methods using high pressure extrusion and homogenization were developed which produce hemoglobin-containing liposomes, approximately one micron or less in diameter with an oxygen-carrying capacity of more than half that of red blood cells (RBCs). These methods were developed for scaling-up liposome-encapsulated hemoglobin (LEH) production. Previously, the lack of adequate ...
Inoue H - - 1989
A new method for the identification of fetal hemoglobin (Hb F) in blood stains by reverse-phase high performance liquid chromatography is described. Differentiation between fetal and adult blood stains is based on the existence of gamma-chain peaks which are characteristics of Hb F. Very few gamma chains appeared on chromatograms ...
Djordjevich L - - 1989
Synthetic erythrocytes (SEs) are made by incorporation of stroma-free hemoglobin (SFH) or lipid-heme into liposomes. They form spontaneously at the interface between the phospholipid material and aqueous solution. SEs are spheroid vesicles with diameters between 0.01 and 4 microns, capable of carrying and delivering oxygen in a manner similar to ...
Tamura T - - 1989
A new compact high-performance instrument for monitoring the oxygenation state of the human newborn are developed. First we applied this instrument to the rat head, and next to the human arm. We could confirm that the volume of Oxy-Hb, Deoxy-Hb and total blood volume were well monitored by using our ...
Hinghofer-Szalkay H - - 1989
Using the "mechanical oscillator technique," the mass density of antecubital venous blood and plasma samples was measured 5-20 times in order to study the influence of postural changes (gravity dependence) on human blood mass density with 0.01 g.L-1 precision, while performing tilt table tests in 17 men. Hemoglobin concentration was ...
Ohba Y - - 1989
An abnormal hemoglobin found in an erythremic blood donor was separable only by isoelectrofocusing, where it was located at the cathodic edge of Hb A. Cation exchange high performance liquid chromatography of a tryptic digest from the total alpha chain revealed splitting of the alpha T-6 peak, although our routine ...
Kiefer C R - - 1988
In an effort to develop a rapid screening immunoassay for the presence of hemoglobin S (Hb S) in cord blood, we have produced a hybridoma (beta s-1) secreting a monoclonal antibody (MAb) with strict specificity for Hb S over hemoglobin (Hb A). A reactivity was observed for hemoglobin C (Hb ...
Claireaux G - - 1988
The effects of deep and acute hypoxia (PwO2 = 25 Torr) on oxygen transport characteristics (Hill number (n) and P50) were investigated in trout at 15 degrees C. When a fish is submitted to such an acute and deep hypoxia, a metabolic acidosis develops as soon as the arterial oxygen ...
Christensen S M - - 1988
A procedure is presented for the preparation of a purified fraction of adult human hemoglobin (HbAo) from one unit of outdated blood. The entire process requires less than 16 h and gives a sterile, endotoxin-free solution of HbAo (approximately 30 g) in a yield of 50%. The solutions are isoionic ...
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