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Gauvrit Sébastien - - 2014
Dicer is an RNase III enzyme that cleaves double stranded RNA and generates functional interfering RNAs that act as important regulators of gene and protein expression. Dicer plays an essential role during mouse development because the deletion of the dicer gene leads to embryonic death. In addition, dicer-dependent interfering RNAs ...
Quick Christopher M CM Texas A&M - - 2014
Lymph flow is the primary mechanism for returning interstitial fluid to the blood circulation. Currently, the adaptive response of lymphatic vessels to mesenteric venous hypertension is not known. This study sought to determine the functional responses of post-nodal mesenteric lymphatic vessels. We surgically occluded bovine mesenteric veins to create mesenteric ...
Fromm Jonathan R JR University of Washington Medical Center, Box 357110, 1959 NE Pacific St, Seattle, WA 98195; e-mail: - - 2014
Objectives: We have recently demonstrated that classical Hodgkin lymphoma (CHL) can be immunophenotyped by flow cytometry (FC), thus obviating the need for immunohistochemistry in many cases. The previously described nine-color assay, however, cannot be used by laboratories that do not have access to a nine- or ten-color flow cytometer. Therefore, ...
Lu Xiaoguang - - 2014
Dai Huang Fu Zi Tang(DHFZT),an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction,acute pancreatitis,cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying ...
Datar Sanjeev A SA University of California, San - - 2014
We have shown decreased pulmonary lymph flow in our lamb model of chronically increased pulmonary blood flow (PBF), created by the in utero placement of an 8mm aortopulmonary shunt. The purpose of this study was to test the hypothesis that abnormal lymphatic function in shunt lambs is due to impaired ...
Marino Daniela D Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, August Forel-Strasse 7, 8008 Zurich, - - 2014
The first bioengineered, autologous, dermo-epidermal skin grafts are presently undergoing clinical trials; hence, it is reasonable to envisage the next clinical step at the forefront of plastic and burn surgery, which is the generation of autologous skin grafts that contain vascular plexuses, preformed in vitro. As the importance of the ...
Rahbar Elaheh E Center for Translational Injury Research, Department of Surgery, University of Texas Health Science Center, Houston, TX, 77030, - - 2014
To assess lymphatic flow adaptations to edema, we evaluated lymph transport function in rat mesenteric lymphatics under normal and increased fluid volume (edemagenic) conditions in situ. Twelve rats were infused with saline (intravenous infusion, 0.2 ml/min/100g body weight) to induce edema. We intravitally measured mesenteric lymphatic diameter and contraction frequency, ...
Haldorsen I S - - 2013
Background:We aimed to study the angiogenic profile based on histomorphological markers in endometrial carcinomas in relation to imaging parameters obtained from preoperative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion-weighted imaging (DWI) and to explore the potential value of these markers to identify patients with poor outcome.Methods:In fifty-four surgically staged ...
Minuti A - - 2013
The aim of the study was to assess the small intestine permeability by using lactulose as a sugar probe and blood metabolites in sheep after a challenge with repeated indomethacin injections. According to a change-over design, 7 adult sheep (4 males, 3 females) were subjected to 4 intramuscular injections (every ...
Muradashvili Nino - - 2013
Abstract Traumatic brain injury (TBI) has been associated with various neurological disorders. However, the role of cerebrovascular dysfunction and its mechanisms associated with TBI are still not well understood. Inflammation is the main cause of vascular dysfunction. It affects properties of blood components and the vascular wall leading to changes ...
Aki Ryoichi - - 2013
Lymph nodes are often the first target of metastatic cancer which can then remetastasize to distant organs. The progression of lymph node metastasis is dependent on sufficient blood supply provided by angiogenesis. In the present study, we have developed a color-coded imaging model to visualize angiogenesis of lymph nodes metastasis ...
Meier Thomas O - - 2013
Introduction: Morphological abnormalities of microvessels are described in psoriasis. However, there are conflicting data as to whether their function is also altered. Objective: Our aim was to study the morphology and function of the lymphatic capillaries of psoriatic skin. Methods: Morphology and permeability of initial lymphatics were studied by microlymphography ...
On Ngoc H - - 2013
The present study characterizes the effects of lysophosphatidic acid (LPA) on blood-brain barrier (BBB) permeability focusing specifically on the time of onset, duration, and magnitude of LPA-induced changes in cerebrovascular permeability in the mouse using both magnetic resonance imaging (MRI) and near infrared fluorescence imaging (NIFR). Furthermore, potential application of ...
Wang Jing - - 2013
Carbon nanotubes (CNTs) find their extensive application as a promising material in medicine due to unique characteristics. However, such materials have been accompanied with potentially hazardous effects on human health. The toxicity of CNTs may vary depending on their structural characteristics, surface properties and chemical composition. To gain insight into ...
Lebau Olaf - - 2013
The removal of middle molecules during continuous extracorporeal detoxification therapies is a scientific field of increasing attention. However, it is difficult to find suitable and inexpensive markers and methods regarding the in vitro clearance of molecules in a weight range between 10 and 20 kDa. We present an easy and ...
Rane Swati - - 2013
Purpose:To extend a commonly used noninvasive arterial spin labeling magnetic resonance (MR) imaging method for measuring blood flow to evaluate lymphatic flow.Materials and Methods:All volunteers (n = 12) provided informed consent in accordance with institutional review board and HIPAA regulations. Quantitative relaxation time (T1 and T2) measurements were made in ...
Lange Matthias - - 2013
Determination of regional blood flow by the injection of microspheres in sepsis models is crucial for the experimental evaluation of the influence of experimental treatment strategies on organ perfusion. However, multiple injections may critically increase the total quantity of microspheres, thereby restricting regional microcirculation and altering the results of blood ...
Wilson John T - - 2013
The lymphatic system plays important roles in protein and solute transport as well as in the immune system. Its functionality is vital to proper homeostasis and fluid balance. Lymph may be propelled by intrinsic (active) vessel pumping or passive compression from external tissue movement. With regard to the former, nitric ...
Soto-Miranda Miguel A - - 2013
Little is known about the anatomy of the lymphatic system in the rabbit with regard to relationships between the lymphatic vessel and lymph node. According to our previous studies in human cadavers and canines, the superficial lymphatic system could be divided into lymphatic territories. The aim of this study was ...
Papakonstantinou Stratos - - 2013
BACKGROUND: Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. The Guava EasyCyte Plus (Guava Technologies, Hayward, CA, US) is the first, personal, bench-top flow cytometer designed to address these limitations. OBJECTIVE: The aim ...
Niu Chun-Yu - - 2013
Disturbances of the microcirculation and abnormal hemorheological properties are important factors that play an important role in disseminated intravascular coagulation (DIC) and result in organ dysfunction or failure. In the present study, we established an animal model of DIC using intravenous Dextran 500 in rats, and used exogenous normal lymph ...
Varin Rémi - - 2013
INTRODUCTION: Defective thrombolysis, a thrombotic risk factor, can be attributed to the formation of a compact clot poorly accessible to fibrinolytic enzymes. Venous thrombi, rich in red blood cells (RBCs), and arterial thrombi containing various amounts of RBCS, plasma and whole blood (WB) clot permeability and degradability were compared. The ...
Lee Sungwoo - - 2013
Fluorescent nanoparticles (FNPs) which were injected into a tumor tissue flowed out through the blood and lymph vessels. The FNPs in blood vessels remained only in the order for few minutes while those in lymph vessels remained for a long time disappearing completely in 25 hours. We found a primo ...
Kesler Cristina T CT E. L. Steele Laboratory, Department of Radiation Oncology, Harvard Medical School and Massachusetts General Hospital, Boston, MA, - - 2013
The lymphatic vasculature plays vital roles in tissue fluid balance, immune defense, metabolism, and cancer metastasis. In adults, lymphatic vessel formation and remodeling occur primarily during inflammation, development of the corpus luteum, wound healing, and tumor growth. Unlike the blood circulation, where unidirectional flow is sustained by the pumping actions ...
Gabr Hanan Sobhy Mohamed - - 2012
Host blood loss by Rhipicephalus sanguineus feedings (weight/tick) ranged from 0.0179 to 0.2469 mg (0.21686 +/- 0.26785) and from 0.00103 to 0.00159 gm (0.001274 +/- 0.000204) respectively for each female and male if fed once. Results obtained herein indicated that if each sheep and or goat in production in Egypt ...
Sweat Richard S RS Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana 70118, - - 2012
Lymphatic and blood microvascular systems play a coordinated role in the regulation of interstitial fluid balance and immune cell trafficking during inflammation. The objective of this study was to characterize the temporal and spatial relationships between lymphatic and blood vessel growth in the adult rat mesentery following an inflammatory stimulus. ...
Castillo-Lopez E - - 2012
The objectives of this trial were to determine the rumen undegradable protein (RUP) of dried distillers grains with solubles (DDGS), to compare the estimates of duodenal bacterial crude protein (BCP) flow using diaminopumelic acid (DAPA) or DNA as bacterial markers, and to estimate duodenal protozoal crude protein (PCP) and yeast ...
Girling Jane E - - 2012
The endometrium has a complex and dynamic blood and lymphatic vasculature which undergoes regular cycles of growth and breakdown. While we now have a detailed picture of the endometrial blood vasculature, our understanding of the lymphatic vasculature in the endometrium is limited. Recent studies have illustrated that the endometrium contains ...
Lee Sungwoo - - 2012
Primo vessels were observed inside the lymph vessels near the caudal vena cava of a rabbit and a rat and in the thoracic lymph duct of a mouse. In the current work we found a primo vessel inside the lymph vessel that came out from the tumor tissue of a ...
Naik Ramachandra S - - 2012
Current methods for measuring acetylcholinesterase (AChE) activities in whole blood use butyrylcholinesterase (BChE)-selective inhibitors. However, the poor selectivity of these inhibitors results in the inhibition of AChE activity to some degree, leading to errors in reported values. The goal of this study was to develop and validate a simple assay ...
Ikomi Fumitaka - - 2012
Lymphatics are a unidirectional transport system that carries fluid from the interstitial space and back into the blood stream. Initial lymphatics take up not only fluid but also high-molecular-weight substances, such as plasma proteins and hyaluronan; immune cells, such as lymphocytes, macrophages, and dendritic cells; and colloidal particles, such as ...
Sacerdoti David - - 2012
Experimental studies show that the heme oxygenase/carbon monoxide system (HO/CO) plays an important role in the homeostasis of circulation and in the pathophysiology of hypertension. No data are available on its role in the control of peripheral circulation in humans. We evaluated the effects of inhibition of HO with stannous ...
Buehler Paul W - - 2011
Abstract Throughout their life span, circulating red blood cells (RBCs) transport oxygen (O(2)) primarily from the lungs to tissues and return with carbon dioxide (CO(2)) from respiring tissues for final elimination by lungs. This simplistic view of RBCs as O(2) transporter has changed in recent years as other gases, for ...
Upile Tahwinder - - 2011
Over 25 years ago Francis reported an association between blood transfusion and worsened cancer prognosis. Subsequently there has been much debate over whether there is in fact such an association, and if so, what is its underlying mechanism. Allogeneic blood transfusion is the most frequent allo-transplantation procedure performed on a routine ...
Sturesson Christian - - 2011
Interstitial laser thermotherapy was used to treat rat liver tumours. The aim was to investigate the influence of temperature and temporary hepatic inflow occlusion on tumour growth and blood perfusion. Liver tumours were treated at 44°C at the tumour border for 30 minutes, hepatic inflow occlusion only, or a combination of ...
Harford-Wright Elizabeth - - 2011
Cancers of the brain are intrinsically more complicated to treat than systemic malignancies due to the unique anatomical features of the brain. The blood-brain barrier prevents chemotherapeutic agents from reaching brain neoplasms, and angiogenesis occurs as the metabolic needs of the tumour increase, thus further complicating treatment. The newly formed ...
Mock Donald M - - 2011
Anemia is a serious problem in critically ill neonates. To investigate the pathophysiology of anemia and responses to red blood cell (RBC) transfusions and erythropoietin therapy, repeated measurement of red blood cell volume (RCV) and blood volume is useful. To extend our previous sheep study in which RBCs were labeled ...
Mock Donald M - - 2010
To investigate the pathophysiology of anemia and responses to red blood cell (RBC) transfusions and erythropoietin, repeated measurement of the circulating red blood cell volume (RCV) would be useful. Ovine erythropoiesis is similar to human erythropoiesis. Accordingly, a method for measuring RCV using either human or sheep RBCs labeled at ...
Bosman Giel J C G M - - 2011
During storage of red blood cell (RBCs) before transfusion, RBCs undergo a series of structural and functional changes that include the exposure of phosphatidylserine (PS), a potent removal signal. It was postulated that, during blood bank storage, the susceptibility to stress-induced PS exposure increases, thereby rendering a considerable fraction of ...
Mock Donald M - - 2011
Safe, accurate methods permitting simultaneous and/or repeated measurement of red blood cell (RBC) survival (RCS) are important to investigate pathophysiology and therapy of anemia. Methods using chromium 51 ((51) Cr)-labeled RBCs are unacceptable for infants, children, and pregnant women. We report RCS measured in vivo using RBCs labeled with several ...
Tissot Jean-Daniel - - 2010
The mechanisms involved in the formation of red blood cell (RBC) microparticles in vivo as well as during erythrocyte storage are reviewed, and the potential role of microparticles in transfusion medicine is described. Microparticles release is an integral part of the erythrocyte ageing process, preventing early removal of RBCs. Proteomics ...
Secomb Timothy W - - 2011
Blood is a concentrated suspension of red blood cells (RBCs). Motion and deformation of RBCs can be analyzed based on knowledge of their mechanical characteristics. Axisymmetric models for single-file motion of RBCs in capillaries yield predictions of apparent viscosity in good agreement with experimental results for diameters up to about ...
Kang Yang Jun - - 2010
A high-precision microfluidic viscometer with a microfluidic channel array composed of 100 indicating channels is demonstrated in this study. The relative viscosity of the sample fluid could be measured by simply counting the number of the indicating channels occupied by the sample and the reference fluids. Using lumped parameter modeling, ...
Pribush Alexander - - 2011
Usually, investigations of erythrocyte aggregation at rest are focused on effects of the strength of erythrocyte-erythrocyte attractive interactions and the volume fraction of the cells, whereas the role of prior flow velocity has not been thoroughly investigated. The aim of this study is to fill this gap. The main conclusions ...
Kaliviotis Efstathios - - 2011
The spatial characteristics of blood viscosity were investigated by combining a newly developed constitutive equation with shear deformation fields calculated from velocity measurements obtained by a μPIV based technique. Blood at physiological hematocrit levels and in the presence of aggregation was sheared in a narrow gap plate-plate geometry and the ...
Zhao Lian - - 2010
Viscosity of blood substitutes is among the important determinants to restore microcirculation. Sodium alginate (SA) is always mentioned as "viscosity modifier" in creating blood substitutes. In the present study, the whole blood was diluted using SA solutions to final hematocrits of 10%, 20%, and 35%, respectively. The whole blood viscosity ...
Canaud Bernard - - 2010
Whole-blood viscosity is increasingly being recognized as a factor implicated in the vascular disease progression in high-risk chronic kidney disease patients. Intermittent hemodialysis and hemodiafiltration sessions, characterized by rapid volume changes and anemia correction by erythropoietin stimulating agents, are favorable conditions for enhancing whole-blood viscosity changes and consequently triggering cardiovascular ...
Bourgeaux Vanessa - - 2010
Hypoxia is a major cause of painful vaso-occlusive crisis in sickle cell disease (SCD). Simple transfusion and red blood cell (RBC) exchange are commonly used as preventive therapies whose aim is to dilute hemoglobin (Hb)S-containing RBCs (SS-RBCs) with normal RBCs (AA-RBCs) to prevent sickling. We hypothesized that the effectiveness of ...
Shim Joon S - - 2010
A disposable on-chip whole blood/plasma separator, which is able to separate plasma from whole human blood by capillary force through a bead-packed microchannel, has been designed, fabricated and characterized in this work. Various sizes of silica beads were slurry-packed through a microchannel using a bump structure which held beads in ...
Weiss D R - - 2011
Background and Objectives  Conventional quality control studies of the shelf life of RBC units do not consider cold chain interruptions that occur during cross-matching or between the release of RBCs from the blood bank and their return from the ward. These interruptions may, however, lead to a considerable loss of ...
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