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Results 301 - 350 of 1198
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Campos Kátia Oliveira - - 2004
Two year-old cloned plants of Dendrobium Second Love were submitted to 25 degrees C (light) and 10 degrees C (dark) under a 12h photoperiod (60 micromol m(-1)s(-1)) for 30 days. The endogenous levels of IAA, ABA, and the cytokinins Z, [9R]Z, iP, and [9R]iP were measured 15, 22, and 30 ...
Paschalidou A K - - 2004
The objective of the present study is to contribute to the understanding of the "ozone weekend effect" as it occurs in the Greater Area of Athens, Greece both in the summer and wintertime. Therefore, weekly cycles of CO, NO and O3 concentrations for the 18-year-period 1983-2000 are studied. Each day ...
Munyiri Florence N - - 2004
At 25 degrees C and under a long-day photoperiod, all 5th instar Psacothea hilaris larvae pupate at the next molt. Under a short-day photoperiod, in contrast, they undergo one or two additional larval molts and enter diapause; the 7th instar larvae enter diapause without further molt. The changes in hemolymph ...
Krzymowski Tadeusz - - 2004
Facts discovered in recent decades have compelled us to revise long-established views on the physiological regulation of cyclic adjustments to the reproductive system in preparation for pregnancy in females. Evidence has been presented to show that changes in the uterine blood supply induced by the oestrogen/progesterone ratio in the blood ...
Sukjumlong S - - 2004
Oestrogen receptor alpha (ERalpha), the main subtype in the uterus, is involved in the regulation of uterine growth/proliferation. A relationship between ERalpha and proliferative activity has been shown in the cyclic sow uterus, but to our knowledge, no study has been carried out on early pregnant sows. Therefore, by means ...
Conrad Kirk P - - 2004
Glomerular filtration rate (GFR) and renal plasma flow (RPF) increase by 40-65% and 50-85%, respectively, during normal pregnancy in women. Studies using the gravid rat as a model have greatly enhanced our understanding of mechanisms underlying these remarkable changes in the renal circulation during gestation. Hyperfiltration appears to be almost ...
Vogel Ida - - 2004
BACKGROUND: Relaxin has been proposed as a hormone involved in the collagen remodeling of the utero-placental unit. MATERIALS AND METHODS: Human fetal membrane explants were incubated with H1 or H2 relaxin for 48 hours and stretched until rupture in a materials testing machine. Co-incubation with a synthetic collagenase inhibitor was ...
Xu Chen - - 2004
AIM: To assess the spatial and temporal expression of germ cell nuclear factor (GCNF) in male mouse germ cells during postnatal development and in sperm before and after capacitation. METHODS: The indirect immunofluorescence method with anti-GCNF antiserum was used to investigate the GCNF expression in mice at day 8, 10, ...
Arosh J A - - 2004
Recognition and establishment of pregnancy involve several molecular and cellular interactions among the conceptus, uterus, and corpus luteum (CL). In ruminants, interferon-tau (IFNtau) of embryonic origin is recognized as the pregnancy recognition signal. Endometrial prostaglandin F(2alpha) (PGF(2alpha)) is the luteolysin, whereas PGE(2) is considered a luteoprotective or luteotrophic mediator at ...
Medan Mohamed - - 2004
Eighteen cyclic Shiba goats were used in this study. Estrus was synchronized with a single injection of 125 microg of a synthetic analogue of prostaglandin F(2)alpha (PGF(2)alpha) after detection of at least one corpus luteum by B-mode ultrasonography. Blood samples were collected from each animal on days 0, 7 and ...
Saint-Dizier Marie - - 2004
The full-length equine luteinizing hormone/chorionic gonadotropin (LH/CG) receptor (eLH/CG-RA) cDNA and two alternatively spliced isoforms (eLH/CG-RB,C) were isolated from luteal tissue and characterized using a combination of reverse transcription-polymerase chain reaction (RT-PCR) and 5'-rapid amplification of cDNA ends. The 680-amino acid full sequence of eLH/CG-RA displayed 87-92% homology with other ...
Kim J G - - 2004
During rapid development of the fetus, levels of high density lipoprotein (HDL) are elevated in pregnant women. The receptor for HDL, scavenger receptor class B type I (SR-BI), mediates selective cholesteryl ester uptake and is highly expressed in the human placenta. Because of the rapid growth of uterus during early ...
Dadarwal D - - 2004
Uterine involution and follicular dynamics during postpartum period were studied ultrasonographically in French jennies. For the study of uterine involution in postpartum jennies (n = 6, Group S), sonographic measurements of different parts of the uterus and endometrium were made at three-day interval, starting from the day of foaling and ...
Deb Kaushik - - 2004
Genital tract infections caused by gram-negative bacteria induce abortion and are one of the most common complications of human pregnancy. This study was carried out to decipher the mechanism of gram-negative bacterial lipopolysaccharide (LPS)-induced pregnancy loss, using a mouse (Park strain) model. Since many of the biological effects of LPS ...
Hayes Eric S - - 2004
Relaxin is a peptide hormone that exerts numerous effects in a variety of tissues across a broad range of species. Although first identified more than 75 years ago interest in relaxin biology has waxed and waned over the years consistent with peaks and troughs of new experimental data on its ...
Fernández Celadilla Lina - - 2005
METHODS: In order to investigate the effect of chronic inhibition of nitric oxide synthesis along pregnancy, pregnant rats were given drinking water alone (control group) or drinking water containing nonselective nitric oxide synthase inhibitor N(omega)-nitro-L-arginine methyl ester (L-NAME; 15 mg/day/rat equivalent to 50 mg/kg/day; treated group), from postmating days 1 ...
Kim J G - - 2004
The objective was to understand how two forms of folate-binding protein interact to accomplish folate transport during pregnancy in swine. Specific folate binding was measured in uterine flushings during the estrous cycle and early pregnancy and in allantoic fluid (secreted form) and placental membranes (membrane form) throughout later pregnancy. In ...
Nakamura Orie - - 2004
To understand the role of IGF-I in murine pregnancy, we studied the reproductive performance of IGF-I overexpressed mice. Fetal loss occurred only in the transfected uterine horn during day 10-15 of pregnancy. The placenta appeared healthy until Day 10 of pregnancy. From day 12, the decidua basalis of the transfected ...
Franco A J - - 2004
Abstract A detailed study of the ontogenesis of deer stomach has not been undertaken to date, and our aim was to sequence several histological phenomena that occur during the ontogenesis of one of the gastric compartments, the rumen. Histomorphometric and immunohistochemical analyses were carried out on 50 embryos and fetuses ...
Raeside James I - - 2004
Because estradiol (E(2)) production by the early equine conceptus is considered crucial to the establishment of pregnancy, the amounts of E(2), estrone (E(1)), and their sulfates (E(2)S, E(1)S) were measured by RIA in yolk-sac fluid of 63 conceptuses collected by transcervical lavage over the period of 11-26 days after ovulation. ...
Reynolds Mark A - - 2004
BACKGROUND: Alterations in sex steroids during pregnancy are associated with the development and exacerbation of reactive lesions involving the gingiva. Currently, few experimental animal models similar to humans are available to examine regulatory pathways involving sex steroids and the periodontium. METHODS: In the present study, we used the baboon as ...
Erünal-Maral Nil - - 2004
The efficacy of cabergoline solely or combined with a PGF2alpha analogue in inducing abortion at different stages of pregnancy was investigated in 18 queens. The queens were assigned to two treatments: Group I ( n=10 )-cabergoline (15 microg/kg; daily, orally) and Group II ( n=8 )-cabergoline (15 microg/kg; daily, orally) ...
Sherwood O David - - 2004
Relaxin has vital physiological roles in pregnant rats, mice, and pigs. Relaxin promotes growth and softening of the cervix, thus facilitating rapid delivery of live young. Relaxin also promotes development of the mammary apparatus, thus enabling normal lactational performance. The actions of relaxin on the mammary apparatus vary among species. ...
Gabriel S - - 2004
The expression pattern of two typical gap junction channel proteins, connexin 43 and connexin 26 (Cx43 and Cx26), was identified in the endometrium of sheep, a species with epitheliochorial type of implantation, by indirect immunohistochemistry during the cyclic phases, early and late pregnancy, and immediately after birth. The extent of ...
Emmanouil-Nikoloussi E N - - 2004
Valproic acid (VPA) is an antiepileptic drug used clinically. Because of its known teratogenic properties VPA is not recommended for women of child bearing age. The present study was designed to assess the effects of VPA on both fetal and maternal organs. Randomized groups of pregnant mice were treated as ...
Sun Tong - - 2004
Cyclooxygenase (COX), a rate-limiting enzyme that produces prostaglandins (PGs) from arachidonic acid, exists in two isoforms, COX-1 and COX-2. PGE2 synthase (PGES) is a terminal prostanoid synthase and can enzymatically convert the cyclooxygenase product PGH2 to PGE2, including two isoforms: microsomal PGES (mPGES) and cytosolic PGES (cPGES). cPGES is predominantly ...
Razdan P - - 2004
Group housing of sows during the mating and gestation period has become the overall common management practice in Sweden. Loose housing is probably less stressful for the animals because it allows them more opportunities to behave naturally, but mixing unfamiliar sows does create a stressful situation due to aggressive interactions, ...
Budge H - - 2004
A range of epidemiological and experimental studies have indicated that suboptimal nutrition at different stages of gestation is associated with an increased prevalence of adult hypertension, cardiovascular disease, and obesity. The timing of prenatal nutrient restriction is important in determining postnatal outcomes-including obesity. The present study, aimed to determine the ...
Goldsmith Laura T - - 2004
Despite the documented importance of the protein hormone relaxin in reproduction in various mammalian species, the role of relaxin in human reproduction is poorly understood, largely because of the lack of studies in women or in suitable non-human primate models. Here we describe the establishment of a non-human primate model ...
Virolainen J V - - 2004
Modified feeding combining the benefits of restricted feeding after ovulation and abundant feeding during implantation in autumn was tested. Three groups of eight gilts were housed with individual feeding stalls and fed 40 MJ per day of a commercial ration. Following insemination gilts were fed 27 MJ per day (LLL) ...
Matsuura Yukiko - - 2004
We analyzed the localization of steroidogenic enzymes (P450 scc, 3 beta HSD, P450 arom and P450 c17) in the corpora lutea of two Hokkaido sika deer (Cervus nippon yesoensis) during the early mating season. Two corpora lutea were found in each female and the timing of formation of the corpora ...
Isobe N - - 2004
The objectives of the present study were to measure oestrone derivatives [oestrone, oestrone sulphate (E1S) and oestrone glucuronide] in sow faeces by direct enzyme-linked immunosorbent assay (ELISA) and to explore the changes of oestrone derivative profile in faeces of miniature pig for demonstrating the possibility of pregnancy diagnosis. Faecal samples ...
Mahadevaswami M P - - 2004
We investigated whether dimethoate, an organophosphorus insecticide, causes abortion or fetal resorption in pregnant albino mice. Graded doses of 16, 20, 24, and 28 mg/kg body weight/d were administered orally from days 7 to day 15 of pregnancy. Laparotomy was performed on day 8 of pregnancy to note the number ...
Menchaca Alejo - - 2004
Timed artificial insemination (TAI) is a very important tool in production systems, as it has a direct impact on cost-efficiency by reducing labour resulting from oestrus detection. However, to make TAI commercially feasible, hormonal treatments need to assure acceptable pregnancy rates and be economically viable. Recent advances in the knowledge ...
Tendron Anaïs - - 2003
Cyclosporin A (CsA) is an immunosuppressive agent used to prevent graft rejection and to treat autoimmune disorders. Successful pregnancies can be achieved among CsA-treated women, although it is known that CsA is nephrotoxic and crosses the human placenta. The aim of this study was to evaluate the harmlessness of CsA ...
Arfuso F - - 2003
There has been much research into the mechanics of angiogenesis and many studies have demonstrated that newly formed vessels regress during angiogenesis. This vascular involution has been shown to involve basement membrane dissolution and endothelial cell apoptosis. The corpus luteum provides an ideal in vivo model to study physiologic angiogenesis ...
Masini Emanuela - - 2004
In animal models of inflammation, the pregnancy hormone relaxin was shown to reduce the recruitment of leukocytes, especially neutrophils, in inflamed tissues. The current study was designed to clarify whether relaxin could inhibit activation of isolated human neutrophils and, if so, whether the nitric oxide (NO) biosynthetic pathway was involved, ...
Christenson Lane K - - 2003
The synthesis of progesterone by the corpus luteum is essential for the establishment and maintenance of early pregnancy. Regulation of luteal steroidogenesis can be broken down into three major events; luteinization (i.e., conversion of an ovulatory follicle), luteal regression, and pregnancy induced luteal maintenance/rescue. While the factors that control these ...
Oshima K - - 2003
Leukemia inhibitory factor (LIF) and macrophage colony stimulating factor (M-CSF), members of the group of hemopoietic cytokines, play a primary role in the control of embryo development and implantation and in the growth of the placenta in humans and mice. Gene expressions of LIF and M-CSF were investigated using quantitative ...
Schmidt C - - 2003
Adrenergic nerve fibres of the mammalian uterus degenerate during pregnancy. The behaviour of peptidergic fibres, such as substance P-positive fibres and of its preferred neurokinin 1 receptor (NK1-R), is poorly studied in the pregnant rat uterus. The present study analysed the changes in substance P immunoreactivity and in the expression ...
Ratnasooriya W D - - 2003
Icon is a water miscible type II synthetic pyrethroid insecticide based on active ingredient lambda cyhalothrin (10% w/w). It is used in Sri Lanka as an adulticidal indoor spray against malaria vector mosquitoes. The goal of this study was to assess the effects of Icon on pregnancy outcome of rats ...
Barna P - - 2003
This study was done in a large Croatian pig-production unit. Blood samples from late-pregnancy gilts were tested by an indirect immunofluorescence-antibody (IFA) serum assay for Lawsonia intracellularis (LI). Ten IFA-positive and 10 IFA-negative gilts were selected. The 2-week-old piglets (97 from positive gilts, and 98 from negative gilts) were inoculated ...
Laws R M - - 2003
Direct observation of 387 embryos in the early stages of development was combined with observations on breeding behaviour and reproductive biology obtained from the published literature, to estimate the timing of births, oestrus, ovulation and implantation, and to derive estimates of the duration of pregnancy, embryonic diapause and active gestation ...
Fowler P A - - 2003
The decline in pulsatile LH secretion and pituitary responsiveness to GnRH as pregnancy advances may be due to non-steroidal factors secreted by the ovine corpus luteum of pregnancy. Corpora lutea from ten ewes on days 70-80 of gestation were homogenized, charcoal-treated and, together with charcoal-treated follicular fluid from superovulated women, ...
Faas M M - - 2003
Pregnancy in the rat may be associated with an activated innate immune system. Therefore, we investigated monocyte function as well as total white blood cell (WBC) counts during the follicular phase of the ovarian cycle, pregnancy and pseudopregnancy in the rat. Rats were equipped with a permanent jugular vein cannula, ...
Longo Monica - - 2003
OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence ...
Ding N-Z - - 2003
The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor delta (PPARdelta) gene in rat uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta mRNA expression in the luminal epithelium was high on day 1 of pregnancy, gradually declined from day 2 ...
Kimmins Sarah - - 2003
We have shown previously that downregulation of intercaruncular stromal integrin alphavbeta3 in bovine endometrium on day 16 of the estrous cycle coincided with the antibody recognition of estrogen receptors (ER) in the luminal epithelium. In pregnancy, these changes were not observed. Our hypothesis was that on day 16 of the ...
Yuen B S J - - 2003
This study aimed to determine for the first time whether leptin can act to alter the structural and functional characteristics of adipose tissue before birth. Leptin (0.48 mg/kg/day) or saline was infused intravenously into fetal sheep for 4 days from either 136 or 137 days of gestation (term=147+/-3 days). Circulating ...
Takeshita Tamotsu - - 2003
OBJECTIVE:Multiple patches of atrophy have been reported in the stria vascularis (SV) in elderly persons with presbycusis The aim of this study was to investigate the correlation between sensorineural hearing loss and this strial condition. MATERIALS AND METHODS: We established a new animal model comprising two small lesions in the ...
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