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Bolnick Jay M JM Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, - - 2014
To use trophoblast cells accumulating in the endocervical canal at the beginning of pregnancy for noninvasive prenatal testing. Prospective, double-blinded test for fetal gender. Academic medical center. Fifty-six women with singleton pregnancies at gestational age 5-20 weeks. Isolation of fetal cells from resident maternal cells in endocervical specimens using anti-human leukocyte ...
Martini Irene - - 2014
Umbilical cord blood (UCB) is an important source of hematopoietic stem cells (HSCs). However, the concentration of cells in cord blood units is limited and this may represent the main restriction to their therapeutic clinical use. The percentage of metabolically active stem cells provides a measure of the viability of ...
Nanjidsuren T T Animal Biotechnology, Graduate School of Bio and Information Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Republic of - - 2014
The aims of this study were to determine the specific site of 20α-HSD expression in the reproductive tissues on day 30 of pregnancy and during pre-parturition. 20α-HSD mRNA was demonstrated to have the highest expression in the placenta on day 30 of pregnancy and in the ovary during pre-parturition. Weak ...
Haig David D Department of Organismic and Evolutionary Biology; Harvard University; Cambridge, MA - - 2014
Fetal microchimerism (FMc) is predicted to promote the fitness of the fetus and maternal microchimerism (MMc) to promote the fitness of the mother. Offspring and mothers benefit from each other's health. Therefore, microchimeric cells should usually not be harmful to their host. However, the evolutionary interests of mothers and offspring ...
Tasripoo K K 1 Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, - - 2014
The world's first cloned swamp buffalo (Bubalus bubalis) derived from adult ear skin fibroblast has been reported. Donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. A total of 39 blastocysts and 19 ...
Stout Molly J MJ Departments of Obstetrics and - - 2014
Abstract Objective: The maternal-fetal interface must modulate immune function to allow tolerance of fetal cells while still reacting to pathogens to suppress infection. Human leukocyte antigen-G (HLA-G) is a class Ib major histocompatibility complex protein involved in maternal-fetal tolerance. We posited that alterations in placental HLA-G expression predispose women to ...
Liu Yang Y Department of Immunology, Binzhou Medical University, Yantai, Shandong, P. R. China, - - 2014
Acute infection with Toxoplasma gondii (T. gondii) during pregnancy is associated with adverse outcomes. The mechanisms that cause this phenomenon are not clear. Regulatory T cells (Tregs) are involved in maternal tolerance, and here we observed a decrease in the absolute numbers of CTLA-4(+) Tregs and PD-1(+) Tregs in spleen ...
Moffett Ashley - - 2014
Pregnancy presents an immunological conundrum because two genetically different individuals coexist. The maternal lymphocytes at the uterine maternal-fetal interface that can recognize mismatched placental cells are T cells and abundant distinctive uterine NK (uNK) cells. Multiple mechanisms exist that avoid damaging T cell responses to the fetus, whereas activation of ...
Yi Hyun Jeong HJ Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, - - 2014
To investigate whether natural killer (NK) cell and autoimmune antibody acts synergistically, by the action of autoantibodies to increase NK cell number and cytotoxicity, to decrease uterine blood flow during early pregnancy in pregnant women with a history of recurrent spontaneous abortion (RSA). Seventy-five pregnant women (between 5 and 7 ...
Ohashi Yasushi Y Department of Nephrology, Faculty of Medicine, Toho University, Tokyo, - - 2014
Juxtaglomerular cell tumor is a rare renal neoplasm. Secondary hypertension with juxtaglomerular cell tumor can be seen in females in their 20s and 30s. We present a case of juxtaglomerular cell tumor during pregnancy. A 32-year-old female was hospitalized for refractory hypertension and nephrotic syndrome in the 23rd gestational week. ...
Unek Gozde G Department of Histology and Embryology, Medical Faculty, Akdeniz University, 07070 Antalya, - - 2014
Placenta is a transitional area making many physiological activities between mother and fetus and therefore, it is a critical organ influencing the outcome of pregnancy. Fetal growth is directly related to placental development. Accurate placental development depends on coordinated action of trophoblasts' proliferation, differentiation and invasion. Information on cell cycle ...
Koga Kaori K Department of Obstetrics and Gynecology, The University of Tokyo, Tokyo, - - 2014
Toll-like receptors (TLRs) form the major family of pattern recognition receptors (PRRs) that are involved in innate immunity. Innate immune responses against microorganisms at the maternal-fetal interface may have a significant impact on the success of pregnancy, as intrauterine infections have been shown to be strongly associated with certain complications ...
Ampomah Paulina P Centre for Medical Parasitology, University of Copenhagen and Copenhagen University Hospital (Rigshospitalet), 2100 Copenhagen Ø, - - 2014
Naturally acquired protective immunity to Plasmodium falciparum malaria takes years to develop. It relies mainly on Abs, particularly IgG specific for Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) proteins on the infected erythrocyte surface. It is only partially understood why acquisition of clinical protection takes years to develop, but it ...
Jeanty Cerine C Eli and Edythe Broad Center of Regeneration Medicine and the Department of Surgery, University of California San Francisco, San Francisco, California, - - 2014
Maternal-fetal cellular trafficking (MFCT) is the bidirectional passage of cells between mother and fetus during pregnancy. This results in the presence of fetal cells in the maternal circulation, known as fetal microchimerism, and maternal cells in the fetal circulation, known as maternal microchimerism. The biologic role of this transplacental cellular ...
Muñoz-Hernandez Rocio R CSIC/Universidad de Sevilla, Unidad Clínico-Experimental de Riesgo - - 2014
Preeclampsia is a pregnancy-related disorder associated with increased cardiovascular risk for the offspring. Endothelial colony-forming cells (ECFCs) are a subset of circulating endothelial progenitor cells that participate in the formation of vasculature during development. However, the effect of preeclampsia on fetal levels of ECFCs is largely unknown. In this study, ...
Emad Ahmed A Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, - - 2014
Physical separation by density gradient centrifugation (DGC) is usually used as an initial step of multi-step enrichment protocols for purification of fetal cells (FCs) from maternal blood. Many protocols were designed but no single approach was efficient enough to provide non-invasive prenatal diagnosis (NIPD). Procedures and methods were difficult to ...
Dall'aglio Cecilia C Department of Veterinary Medicine, Perugia, Italy. Electronic address: - - 2014
The aim of the present study was to examine the presence and distribution of cells that express immunopositivity for orexin A (OXA) and its type 2 receptor (OX2R) in the dog placenta toward the end of pregnancy using immunohistochemical techniques. In the placental fetal portion, a few OXA and OX2R-positive ...
Kim S H SH Animal Biotechnology, Graduate School of Bio and Information Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Republic of - - 2014
The enzyme 20α-hydroxysteroid dehydrogenase (20α-HSD) catalyzes the conversion of progesterone to its inactive form, 20α-hydroxyprogesterone, and this enzyme has an important role in the regulation of luteal function in mammals. It has previously been determined that the 20α-HSD gene is primarily expressed by large luteal cells during the late stage ...
Mahmood Uzma U Anu Research Centre; Department of Obstetrics and Gynaecology; University College Cork; Cork University Maternity Hospital; Cork, - - 2014
Fetal cells persist in mothers for decades after delivery: in a phenomenon called fetal microchimerism. While persistent fetal cells were first implicated in autoimmune disease, parallel studies in animal and human pregnancy now suggest that microchimeric fetal cells play a role in the response to tissue injury. The aim of ...
McDonald-Burrows Zoë - - 2014
Gestational trophoblastic tumours are a rare form of malignancy, which in the majority of cases arise from abnormal trophoblast cells formed in a complete molar pregnancy. These tumours are extremely sensitive to chemotherapy and high cure rates approaching 100% can be expected. The disease is usually limited to the uterus ...
Eskicioglu Fatma - - 2014
There are no well-defined findings about reasons for first trimester abortion in some pregnancy cases. Selectins are cell adhesion proteins which are important for blastocyst implantation in the decidua. The goal of the study was to investigate the role of selectins in first trimester pregnancy loss by immunohistochemistry. Decidual and ...
Nakai Michiko M Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, - - 2014
In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF ...
Navas Paola Beatríz PB CCT MENDOZA CONICET, IMBECU, Capital, Mendoza, Argentina ; - - 2014
Background: Progesterone (P4) is the main steroid secreted by the corpora lutea (CL) and is required for successful implantation and maintenance of pregnancy. Although adequate circulating levels of thyroid hormone (TH) are needed to support formation and maintenance of CL during pregnancy, TH signaling has not been described in this ...
French Clare E CE aPopulation, Policy and Practice, UCL Institute of Child Health bSchool of Health Sciences, City University London, London, - - 2014
During their second pregnancy with diagnosed HIV (n = 1177), two-fifths of women in the United Kingdom/Ireland not on antiretroviral therapy (ART) at conception had an immunological indication for treatment (CD4 <350 cells/μl), of whom nearly half had CD4 at least 350 cells/μl in their previous pregnancy. Those initiating ART during pregnancy had a ...
Schumacher Anne A Department of Experimental Obstetrics & Gynecology, Medical Faculty, Otto-von-Guericke University, Magdeburg, - - 2014
Pregnancy still represents one of the most fascinating paradoxical phenomena in science. Immediately after conception, the maternal immune system is challenged by the presence of foreign paternal antigens in the semen. This triggers mechanisms of recognition and tolerance that all together allow the embryo to implant and later the fetus ...
Xu Peng P State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, - - 2014
Preeclampsia is a major cause of maternal and fetal mortality and morbidity worldwide. The differential expression of several microRNAs (miRNAs) has been found in preeclamptic placentas. However, great conflict exists regarding this aspect, and detailed examinations have largely been lacking of miRNA profiles in different parts of the placenta and ...
Alijotas-Reig Jaume J Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d'Hebron University Hospital, Spain; Department of Medicine, Faculty of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain. Electronic address: - - 2014
Because maternal alloreactive lymphocytes are not depleted during pregnancy, local and/or systemic mechanisms have to play a key role in altering the maternal immune response. Peripheral T regulatory cells (pTregs) at the maternal-foetal interface are necessary in situ to prevent early abortion, but only those pTregs that have been previously ...
Mao Jun J Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu, - - 2014
Non-invasive prenatal testing (NIPT) by massively parallel sequencing is a useful clinical test for detection of common fetal aneuploidies. While the accuracy of aneuploidy detection can approach 100%, results discordant with the fetus are occasionally reported. In this study we investigated the basis of a discordant T21 positive and T18 ...
Kim Dong Jae DJ Department of Obstetrics and Gynecology, College of Medicine, Konyang University, Seo-gu, Daejeon, - - 2014
Th17 cells and Foxp3(+) regulatory T (Treg) cells have been proposed as new risk factors for recurrent pregnancy loss (RPL). Intravenous immunoglobulin G (IVIG) was reported to modulate various immune cells. In this study, we investigated the effect of IVIG on the levels of Th17 and Treg cells and pregnancy ...
Schminkey Donna L DL University of Virginia, United States. Electronic address: - - 2014
Recent research challenges long-held hypotheses about mechanisms through which pregnancy induces maternal immune suppression or tolerance of the embryo/fetus. It is now understood that normal pregnancy engages the immune system and that the immune milieu changes with advancing gestation. We suggest that pregnancy mimics the innate immune system's response to ...
Wambach Carrie M CM Division of Reproductive Endocrinology and Infertility, Department of OB/GYN, Cedars-Sinai Medical Center, Los Angeles, CA, - - 2014
To determine the interplay between fetal antigenicity and local maternal factors in determining reproductive tract T regulatory (Treg) cell accumulation during pregnancy. Examination of maternal Treg composition in the uterus, cervix, and uteroplacental interface (UPI) of murine syngeneic and allogeneic pregnancies and non-pregnant controls by flow cytometry. The impact of ...
Saddick Salina Y SY Biological Studies Dept., Faculty of Science, King Abdulaziz University, PO Box 42671, Jeddah 21551, Saudi - - 2014
The present study is designed to demonstrate the ovarian surface epithelial cells' (OSE) estrogen receptor α (ERα) and progesterone receptor (PR) during pregnancy and estrous cycle in rat. Moreover, determination of the levels of plasma progesterone, estradiol, FSH and LH was also made. The levels of plasma progesterone, estradiol, FSH ...
Sorrentino Mario M 1Ospedale Civile di Latisana, Latisana (UD), - - 2014
A 27-year-old women requesting assistance for an unspecified abdominal pain localized in the right flank that worsened after a recent delivery was discovered to have a solid mass in the upper pole of her right kidney. Radiological findings showed benign characteristics but without a clear diagnosis. Subsequently, a laparotomic nephron-sparing ...
Bills V L VL Fetal Medicine Unit, St Michael's Hospital, University Hospitals Bristol Foundation NHS Trust, Southwell Street, Bristol BS2 8EG, United - - 2014
Red cell alloimmunisation involves the transplacental movement of maternally derived red cell antibodies into the fetal circulation, causing red cell haemolysis, fetal anaemia and ultimately fetal death. Current standard UK practice is to prevent sensitisation to the D antigen by administering anti-D at about 28weeks' gestation to all RhD negative ...
Schumacher Anne A Department of Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, Magdeburg, - - 2014
Pregnancy hormones were proposed to be crucially involved in fetal tolerance. Recently, we showed that human chorionic gonadotropin (hCG) not only increases the number and activity of regulatory T cells (Treg) but also retains tolerogenic dendritic cells (DCs). Here, we investigate whether the highly homologous luteinizing hormone (LH) modulates Treg ...
Menon Ramkumar R Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine and Perinatal Research, The University of Texas Medical Branch at Galveston, Galveston, TX, - - 2014
The most common DNA lesion generated by oxidative stress (OS) is 7, 8-dihydro-8-oxoguanine (8-oxoG) whose excision repair is performed by 8-oxoguanine glycosylase (OGG1). We investigated OGG1 expression changes in fetal membranes from spontaneous preterm birth (PTB) and preterm premature rupture of the membranes (pPROM) and its changes in vitro in ...
Xin Lijun L Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH - - 2014
Pregnancy stimulates induced Foxp3 expression among maternal CD4(+) T cells with fetal specificity. Although sustained maternal regulatory CD4(+) T cell (Treg) expansion is essential for maintaining fetal tolerance during pregnancy, the necessity for Foxp3(+) cells with fetal specificity remains undefined. In this study, we demonstrate that mitigating Treg differentiation among ...
Labarrere C A CA CBL Partners for Life, Indianapolis, IN, United States; Indiana University School of Medicine, Indianapolis, IN, United States; Indiana University Health Methodist Research Institute, Indianapolis, IN, United States. Electronic address: - - 2014
Massive chronic intervillositis (MCI), also known as chronic intervillositis of unknown etiology, is a placental lesion associated with massive infiltration of mononuclear cells in the intervillous space, poor perinatal outcome, and high rate of recurrence. Our previous demonstration of increased syncytiotrophoblast (st) intercellular adhesion molecule-1 (ICAM-1) expression in villitis lesions ...
Moussa Mahmoud M Obstetrics and Gynecology, Faculty of Medicine, Ain Shams University, Cairo, - - 2014
Adult-onset Still's disease (AOSD) is a rare inflammatory disease of unknown etiology that can present for the first time during pregnancy. Pure red cell aplasia (PRCA) is another rare condition that seldom is in association with AOSD. Herein, we have reported a newly diagnosed case with AOSD and PRCA in ...
Emad Ahmed A Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, PQ, - - 2014
Detection of rare fetal cells (FCs) in the maternal circulation could be used for non-invasive prenatal diagnosis. Considering that FCs in maternal blood are present in extremely low frequency, manual scanning is cumbersome, time-consuming and unsuitable for clinical applications. As an alternative, we optimized a custom-made classifier for automatic detection ...
Hayashi Kazuhiko K Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi - - 2014
The causal relationship between severe allergic conditions and successful pregnancy remains unclear. We aimed to evaluate reproductive performance in an experimental mouse model of atopic disease (AD), and the appearance of uterine natural killer (uNK) cells that have crucial roles in placental formation was examined. In the NC/Nga pregnant mice ...
Mandal Prakas Kumar PK Department of Hematology, NRS Medical College and Hospital, 138, AJC Bose Road, Kolkata, 700014, - - 2014
Non-Hodgkin lymphoma associated with pregnancy is rare. Rituximab based chemotherapy is now considered the standard of care and considered safe for the treatment of diffuse large B-cell lymphoma (DLBCL) during pregnancy but little is known about its safety profile on the fetus. A 32 y primigravida was diagnosed as DLBCL at ...
Papamitsou Theodora T Laboratory of Histology and Embryology, Aristotle University, Thessaloniki, Greece. - - 2014
Introduction: Recurrent pregnancy loss (RPL) of unknown etiology is correlated with immunological alterations during pregnancy. Normally, changes in leukocyte subpopulations and HLA expression take place in pregnant uterus in order to tolerate the semi-allogenic embryo. Objective: Our research tries to enlighten the immunological changes that take place in the uterus ...
Schober Linda L Department of Obstetrics and Gynecology, University of Heidelberg, - - 2014
Physiological changes during normal pregnancy are characterized by an inflammatory immune response and insulin resistance. Therefore, we hypothesize that gestational diabetes mellitus (GDM) may be caused by an inappropriate adaption of the maternal immune system to pregnancy. In this study we examined the role of regulatory T cell (Treg) differentiation ...
Sohlberg S S Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden. Electronic address: - - 2014
Preeclampsia affects about 3% of pregnancies and the placenta is believed to play a major role in its pathophysiology. Lately, the role of the placenta has been hypothesised to be more pronounced in preeclampsia of early (<34 weeks) rather than late (≥34 weeks) onset. (31)P Magnetic Resonance Spectroscopy (MRS) enables ...
Schmerse Franziska F F Schmerse, Experimental Obstetrics and Gynecology, Otto-von-Guericke University, Magdeburg, - - 2014
Transgenic mice expressing fluorescent proteins in specific cell populations are widely used for the study of in vivo behavior of these cells. We have recently reported that uterine mast cells (uMCs) are important for implantation and placentation. However, their in vivo localization in uterus before and during pregnancy is unknown.Here, ...
Alijotas-Reig J J Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d'Hebron University Hospital, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain; Department of Medicine, Faculty of Medicine, Universitat Autonoma, Barcelona, Spain. Electronic address: - - 2014
The maternal immune system needs to adapt to tolerate the semi-allogeneic conceptus. Since maternal allo-reactive lymphocytes are not fully depleted, other local/systemic mechanisms play a key role in altering the immune response. The Th1/Th2 cytokine balance is not essential for a pregnancy to be normal. The immune cells, CD4+CD25+Foxp3+, also ...
Grati Francesca R FR Research & Development, Cytogenetics, Molecular Cytogenetics and Molecular Biology, TOMA Advanced Biomedical Assays, S.p.A., Busto Arsizio (VA), - - 2014
Purpose:Noninvasive prenatal screening for fetal aneuploidy analyzes cell-free fetal DNA circulating in the maternal plasma. Because cell-free fetal DNA is mainly of placental trophoblast origin, false-positive and false-negative findings may result from placental mosaicism. The aim of this study was to calculate the potential contribution of placental mosaicism in discordant ...
Wallace A E AE Division of Biomedical Sciences, St. George's, University of London, Cranmer Terrace, London SW17 ORE, - - 2014
Are the concentrations of factors secreted by decidual natural killer (dNK) cells from pregnancies at high risk of poor spiral artery remodelling different to those secreted from pregnancies at low risk? Expression levels of PLGF, sIL-2R, endostatin and angiogenin were significantly increased by dNK cells from high-risk pregnancies, and angiogenin ...
Cheng Xin - - 2014
Glucocorticoids are important regulators of cell differentiation and mesenchymal cell lineage commitment during skeletogenesis. In clinical practice, it has been difficult to study the effects of glucocorticoids on target tissues because patients taking glucocorticoids often suffer from adverse skeletal effects. Dexamethasone (Dex) is a long-acting synthetic corticosteroid hormone that ranks ...
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