Search Results
Results 301 - 350 of 1424
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Dal Canto M B - - 2006
In March 2004, a new law was introduced in Italy to regulate assisted reproduction; at present it is impossible to use more than a maximum of three oocytes per IVF cycle, nor can embryos or prezygotes (2PN cells) be selected or cryopreserved. The prohibitions introduced by the new law have, ...
Cha Kwang Yul - - 2006
In vitro maturation (IVM) programme has developed a culture technique for supporting the growth and maturation of immature oocytes since late of 1980s and has become important in ART methods. The establishment of an IVM programme can yield many advantages. Patients suffering from congenital or postnatal reproductive disorders, such as ...
Khattab Sherif - - 2006
In the present study, which includes 287 participants, metformin has been used by women undergoing IVF/intracytoplasmic sperm injection for more than 5 weeks before and during treatment and during luteal phase. There was no significant difference in number of gonadotrophins used, days of stimulation, number of oocytes retrieved, and number ...
Balmaceda Jose P - - 2006
It is agreed that reliability of hormonal products, starting with recombinant technology, has improved uniformity of ovarian response to a specified dosage, and that this has resulted in better pregnancy rates. Nevertheless, IVF cycle cancellation rate has not experienced a substantial decline in the last 5-10 years. We feel that ...
Lozano Daniel Humberto Mendez - - 2006
To evaluate the importance of follicular flushing on semi natural cycles IVF, we have compared prospectively the reproductive potential of oocytes obtained from follicular fluid (group A, n = 79) to those obtained from follicular flushing (group B, n = 47) in 146 oocyte pick-ups. The groups A and B ...
Katayama Mika - - 2006
Mitochondrial distribution and microtubule organization were examined in porcine oocytes after parthenogenesis, fertilization and somatic cell nuclear transfer (SCNT). Our results revealed that mitochondria are translocated from the oocyte's cortex to the perinuclear area by microtubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate from ...
Oktay Kutluk - - 2006
OBJECTIVE: To determine the efficiency of oocyte cryopreservation relative to IVF with unfrozen oocytes. DESIGN: Meta-analysis. SETTING: Academic assisted reproduction center. PATIENT(S): Results of all reports from January 1997 to June 2005 with the patients undergoing IVF-intracytoplasmic sperm injection (ICSI) with cryopreserved cycles between 1996 and 2004 were compared with ...
Otsuki Junko - - 2006
A complete failure of meiotic maturation occasionally occurs following human chorionic gonadotrophin administration during IVF-intracytoplasmic sperm injection (ICSI) cycles. ICSI on day 1 is commonly used to allow maturation in culture. However, if the oocytes become mature in the evening soon after their recovery but ICSI is delayed until the ...
Escribá María-José - - 2006
OBJECTIVE: To determine whether the initiation of P supplementation as artificial luteal phase support (day -1, day 0, or day +1 of egg donation) in extensive programs of ovum donation influences cycle cancellation, pregnancy outcome, and implantation rate in day 3 embryo transfers. DESIGN: Prospective randomized trial. SETTING: Oocyte donation ...
Ptak Grazyna - - 2006
BACKGROUND: The nuclear compartment has been proposed as responsible for the developmental arrest of prepubertal mouse oocytes while the studies on prepubertal sheep and cow oocyte model suggested the cytoplasm immaturity accounts for this failure. METHODS: The apparent disagreement on the causes of developmental defects between these two species prompted ...
Vlaisavljević Veljko - - 2006
In patients with polycystic ovary syndrome (PCOS), the proportion of optimal embryos-blastomere number and fragmentation rate-derived from in vitro-matured oocytes after the hCG priming protocol was higher (43.3%) than the proportion of optimal embryos derived from oocyte in vitro maturation with the FSH priming protocol (11.1%). The embryos derived from ...
Ghetler Yehudith - - 2006
OBJECTIVE: To examine the effect of the commonly used oocyte cryopreservation protocol on the cortical granules (CGs) of human immature germinal vesicle (GV) and mature metaphase II (MII) oocytes. DESIGN: Laboratory study. SETTING: IVF unit. INTERVENTION(S): Unfertilized, intracytoplasmic sperm injected (ICSI) oocytes, and immature oocytes were cryopreserved using a slow ...
Chamayou S - - 2006
In Italy, the restrictive IVF law generalizes the indication for oocyte freezing for surplus oocytes in 78.5% of in-vitro assisted reproductive cycles. With a view to understanding better what the prospects for intracytoplasmic sperm injection (ICSI) on frozen-thawed oocytes might be, the consequences of freeze-thaw procedures on fertilization, cleavage rates ...
Branigan Emmett F - - 2006
OBJECTIVE: The purpose of this study was to compare the effectiveness of a thorough sonographic oocyte retrieval to a routine in vitro fertilization retrieval to induce ovulation, pregnancy, and endocrine changes in patients with polycystic ovarian syndrome in their post-in vitro fertilization cycles. STUDY DESIGN: Sixty-four patients from a tertiary ...
Lin Yu-Hung - - 2006
Immature oocyte retrieval followed by in vitro maturation (IVM) opens a new horizon for modern assisted reproductive technologies (ART). Recent studies in IVM make it a feasible alternative to in vitro fertilization. Antral follicle count is correlated with the pregnancy rate, so women with polycystic ovarian syndrome or polycystic ovaries ...
Bodri D - - 2006
Little information is available on the outcome of controlled ovarian hyperstimulation (COH) using GnRH antagonist in oocyte donation cycles especially in comparison with the short GnRH agonist protocol. This study was aimed at comparing the two stimulation protocols in oocyte donation (OD) cycles. A total of 113 donors randomly received ...
Rosendahl M - - 2006
Autotransplantation of frozen/thawed ovarian tissue in women undergoing cancer therapy has so far led to the birth of two healthy babies. In both cases, it can be discussed whether the fertilized oocyte originated from the transplant or from the native ovary. We now present a biochemical pregnancy achieved after heterotopical ...
Velilla Esther - - 2006
The aim of this study was to evaluate mitochondrial distribution during in vitro maturation (at 0, 15, 20, and 27 hr of IVM) and fertilization of prepubertal goat oocytes compared to mitochondrial distribution of ovulated and in vitro fertilized oocytes from adult goats. Oocytes from prepubertal goats were recovered from ...
Fujihira Takuma - - 2006
The present study investigated the effects of the sexual maturity of oocyte donors on in vitro maturation (IVM) and the parthenogenetic developmental capacity of fresh minke whale oocytes. The effects of cytochalasin B (CB) pretreatment and two types of cryoprotectant solutions (ethylene glycol (EG) or ethylene glycol and dimethylsulfoxide (EG ...
Tharasanit T - - 2006
Oocyte cryopreservation is a potentially valuable technique for salvaging the germ-line when a valuable mare dies, but facilities for in vitro embryo production or oocyte transfer are not immediately available. This study examined the influence of maturation stage and freezing technique on the cryopreservability of equine oocytes. Cumulus oocyte complexes ...
Melo M A B - - 2006
BACKGROUND: Several evidences indicate that premature luteinization (PL) may affect IVF outcome. The primary end-point of the present study was to verify the effect of PL on the pregnancy rate (PR) of our oocyte-donation programme. METHODS: PL was defined as serum progesterone > or = 1.2 ng/ml on the day ...
West M - - 2007
Porcine FSH/LH stimulation successfully induced development of multiple large (>or=4mm) antral follicles in 10 of 11 common wombats. A mean of 5.5 metaphase II (MII) oocytes were aspirated from wombats that were stimulated during the follicular phase of the oestrous cycle (n=3) or after pouch young removal (n=3). Three subadults ...
Motosugi Nami - - 2006
Knowledge about the mechanism that establishes embryonic polarity is fundamental in understanding mammalian development. In re-addressing several controversial claims, we recently proposed a model in which mouse embryonic polarity is not specified until the blastocyst stage. Before fertilization, the fully differentiated oocyte has been characterized as "polarized," and we indeed ...
Ng Ernest H Y - - 2006
OBJECTIVES: The management of poor ovarian responders remains a great challenge in in vitro fertilization (IVF) treatment. This study compared implantation and pregnancy rates among women who developed <or=3 dominant follicles of >or=16 mm in diameter and those who had >3 dominant follicles after ovarian stimulation. STUDY DESIGN: Retrospective study. ...
Morgia Francesco - - 2006
OBJECTIVE: This study was conducted to determine whether N-hydroxyethylpiperazine-N-ethanesulfonate (HEPES)-buffered medium used for the microinjection of sperm into oocytes may be detrimental for the embryo. DESIGN: Controlled randomized study. SETTING: Private IVF center. PATIENT(S): Women (n = 708) undergoing ICSI. INTERVENTION(S): The women were randomized into two study groups: 2,204 ...
Rodrigues B A - - 2006
The potential benefits of assisted reproduction techniques, such as in vitro maturation (IVM) and in vitro fertilization (IVF) in canids, are linked to the protection and saving of species threatened by extinction due to worldwide habitat destruction and pollution. In both domestic and wild species, these technologies will form the ...
Borini Andrea - - 2006
Storing supernumerary embryos and transferring them later fully utilizes the reproductive potential of retrieved oocytes, allowing a significant increase in the overall number of pregnancies achieved from a single cycle of ovarian stimulation treatment. As an alternative to embryo cryopreservation, preservation of unfertilized oocytes has been proposed to maximize clinical ...
Nagy Zsolt P - - 2006
OBJECTIVE: To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI). DESIGN: Prospective clinical trial. SETTING: Private reproductive medical center. PATIENT(S): Thirty patients undergoing IVF. INTERVENTION(S): Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI, embryo culture, and embryo transfer. MAIN ...
Kok Jeroen D - - 2006
OBJECTIVE: To investigate the possible negative effects of a strong ovarian response on oocyte quality. DESIGN: Retrospective study. SETTING: Tertiary academic center. PATIENT(S): A total of 1,894 women (IVF, n = 1,544; ICSI, n = 350) who underwent their first ovarian stimulation cycle during the period 1995-2002. INTERVENTION(S): Standardized controlled ...
Raju G A Rama - - 2006
A 28-year-old woman with mullerian agenesis presented with primary infertility and was considered for laparoscopic oocyte retrieval and in-vitro fertilisation. Her 27-year-old younger sister served as a gestational carrier. The patient underwent ovarian stimulation and 11 mature oocytes were retrieved by laparoscopy. After successful in-vitro fertilisation, two embryos were transferred ...
Kanaya Hiroyuki - - 2006
The incidence of monozygotic twinning (MZT) appears to be increasing within the field of assisted reproductive technology (ART), although the factors contributing to the phenomenon are still far from being identified. On the contrary, in vitro maturation (IVM) of oocytes is becoming more accepted and more and more babies have ...
Santos Teresa Almeida - - 2006
OBJECTIVE: To determine the content of mitochondrial DNA (mtDNA) in oocytes from a range of patients with fertilization success and failure. DESIGN: Analysis of mtDNA content in fertilized and unfertilized oocytes and embryos by real-time polymerase chain reaction (PCR). SETTING: University hospital infertility and research center. PATIENT(S): Fifty-four women seeking ...
Montag Markus - - 2006
OBJECTIVE: To document the possibility to double-cryopreserve human metaphase II and pronuclear oocytes. DESIGN: Case report. SETTING: University-based IVF center. PATIENT(S): A couple with primary andrological infertility. INTERVENTION(S): Pronuclear stage oocytes derived after sperm injection into frozen-thawed metaphase II oocytes were cryopreserved again for later transfer. MAIN OUTCOME MEASURE(S): Post-thaw ...
Yamamoto Ikuko - - 2006
Intercellular communication plays a pivotal role in regulating and coordinating oocyte meiosis and fertilization, key triggers for embryonic development. The nematode Caenorhabaditis elegans has emerged as an important experimental paradigm for exploring these fundamental reproductive processes and their regulation. The oocytes of most animal species arrest during meiotic prophase and ...
Warzych E - - 2007
The objective of this study was to compare the effect of different supplements to the basic IVM medium (TCM199) on the efficiency of cattle oocyte maturation and blastocyst production, and the incidence of apoptosis in both oocytes and blastocysts. Two protein supplements (FBS and fafBSA) and a macromolecule (PVP40) were ...
Pope C E - - 2006
Over a 5-year interval, multiple laparoscopic oocyte retrievals were done in fishing cats (Prionailurus viverrinus), caracals (Caracal caracal) and domestic cats after ovarian stimulation with gonadotropins. From 21 retrievals in five fishing cats, 579 preovulatory oocytes (mean = 27.6) were recovered and 348 embryos were produced in vitro (mean = ...
Kan Anita - - 2006
BACKGROUND: Normal ovarian responders who have follicles with good vascularity shown by power Doppler scanning are associated with a better pregnancy rate following IVF treatment. This study evaluated the significance of perifollicular vascularity and follicular fluid hormonal profile in poor responders who developed < or = 3 dominant follicles. METHODS: ...
Pau Elena - - 2006
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is a life-threatening condition associated with ovarian stimulation. Its pathophysiology is unknown and its treatment continues to be empirical. Early (E)- and late (L)-OHSS occur in women at risk, though not in all cases. Vascular endothelial growth factor (VEGF) is related to increased vascular permeability ...
Rybouchkin Andrei - - 2006
Before fertilization, chromatins of both mouse oocytes and spermatozoa contain very few acetylated histones. Soon after fertilization, chromatins of both gametes become highly acetylated. The same deacetylation-reacetylation changes occur with histones of somatic nuclei transferred into enucleated oocytes. The significance of these events in somatic chromatin reprogramming to the totipotent ...
da Paz Regina Celia Rodrigues - - 2006
Exogenous gonadotropins are used to stimulate ovarian follicular growth and ovulation in mammalian species, including wild cats. However, successes in application of assisted reproduction techniques in nondomestic felids have been sparse. Our objectives were to assess the effectiveness of alternating gonadotropin regimens on ovarian responses. Five adult female ocelots and ...
Thouas George A - - 2006
Mitochondrial dysfunction may be acquired or inherited by oocytes without detectable morphological abnormalities. This pathology may account for some examples of unexplained pregnancy loss in women following transfer of morphologically normal in vitro fertilization (IVF) embryos. The present study was intended to determine whether sublethal mitochondrial injury in mouse oocytes ...
Roth Thierry Christophe - - 2006
OBJECTIVE: Myasthenia gravis (MG) affects women at childbearing age. Therefore, the question arises if these patients should become pregnant and if thymectomy has a positive effect on the course of MG in pregnant patients. METHODS: Fifteen pregnancies had been followed retrospectively. All patients underwent transsternal radical thymectomy for MG. The ...
van der Westerlaken Lucette - - 2006
OBJECTIVE: To determine whether patients with borderline semen should be treated with conventional IVF or intracytoplasmic sperm injection (ICSI). DESIGN: Randomized study. SETTING: A university medical center in The Netherlands. PATIENT(S): One hundred six couples with borderline semen who were undergoing IVF and ICSI on sibling oocytes. INTERVENTION(S): Performing IVF ...
Nagano Masashi - - 2006
We investigated the relationship between the morphology of oocytes collected from small antral follicles and their developmental capacity. Immature oocytes were classified into seven groups and cultured in vitro for maturation (IVM), fertilization (IVF) and development to blastocysts (IVC). After IVF, sperm penetration and normal fertilization rates were higher in ...
Oral Ozay - - 2006
To investigate the impact of oxidative stress on pregnancy success by monitoring malondialdehyde levels in follicular fluid. Forty five couples were enrolled in this prospective study. Following long protocol of GnRH analogues and r-FSH treatment, oocyte retrieval and intracytoplasmic sperm injection were performed. Malondialdehyde levels were assayed by thiobarbutiric acid ...
Coll Oriol - - 2006
A study on in-vitro fertilization (IVF) was conducted among HIV-infected women. In these patients, a reduced pregnancy rate after IVF was observed if the patient's own oocytes were used. However, no significant reduction in the pregnancy rate was found if donated oocytes were used. The CD4 lymphocyte count was independently ...
Deb Kaushik - - 2006
Pregnancy begins with fertilization of the ovulated oocyte by the sperm. After fertilization, the egg undergoes time-dependent mitotic division while trying to reach the blastocyst stage and the uterus for implantation. Uterine preparation for implantation is regulated by coordinated secretions and functions of ovarian sex steroids. The first sign of ...
Sasseville Maxime - - 2006
BACKGROUND: The ovulatory surge of gonadotropins triggers oocyte maturation and rupture of the ovarian follicle. The resumption of nuclear maturation in the oocyte from the prophase stage is characterized by germinal vesicle breakdown (GVBD). It has previously been shown that specific inhibition of cAMP degradation by PDE3 prevents the resumption ...
Iwamatsu Takashi - - 2006
Using the S-rR strain of the medaka Oryzias latipes, we examined the effect of a non-aromatizable androgen on sex determination. Intrafollicular immature oocytes isolated before breakdown of the germinal vesicle were incubated in the presence of 17alpha-methyldihydrotestosterone (MDHT) for about 10 h during their maturational period. At the end of ...
Chen Naiqing - - 2006
This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing pre-equilibrated IVM medium and transported for 5 ...
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