Search Results
Results 201 - 250 of 1426
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Schutte Joke M - - 2008
BACKGROUND: The percentage of women giving birth after the age of 35 increased in many western countries. The number of women remaining childless also increased, mostly due to aging oocytes. The method of oocyte donation offers the possibility for infertile older women to become pregnant. Gestation after oocyte-donation-IVF, however, is ...
Hannoun Antoine - - 2008
OBJECTIVE: The objective of this study is to assess the effect of two methods of vaginal scrubbing before egg retrieval on the outcome of in vitro fertilization-embryo transfer (IVF-ET) cycles. METHOD: 721 consecutive cycles of IVF-ET, at the American University Hospital of Beirut, were randomized prospectively into one of two ...
Check J H - - 2008
PURPOSE: The need for freezing oocytes has been established for females undergoing potential therapy that could damage their ovarian egg reserve, ethical or religious reasons (not having excess embryos frozen) or women nearing the age of lower fecundity but not married and not ready to use donor sperm. Applying the ...
Varghese Alex C - - 2008
Cryopreservation of ovarian tissue and in vitro follicle maturation are two emerging techniques for fertility preservation, especially in cancer patients. These treatment regimes are opening up more options and allow for more suitable choices to preserve fertility according to the patient's specific circumstances. If these technologies are to become widely ...
Dumesic Daniel A - - 2008
Folliculogenesis is a complex process, in which multiple endocrine and intraovarian paracrine interactions create a changing intrafollicular microenvironment for appropriate oocyte development. Within this microenvironment, bidirectional cumulus cell-oocyte signaling governs the gradual acquisition of developmental competence by the oocyte, defined as the ability of the oocyte to complete meiosis and ...
Dirican Enver K - - 2008
We report the successful outcome of intracytoplasmic sperm injection (ICSI) treatment in two siblings with familial globozoospermia. After controlled ovarian hyperstimulation and oocyte pick-up, retrieved oocytes were mechanically activated before ICSI and a fertilization rate of 33.3% was achieved in the first case. The second couple underwent ICSI without oocyte ...
Kumbak Banu - - 2008
OBJECTIVE: To investigate whether the space-occupying effect of an endometrioma, rather than endometriosis itself, affects results in in vitro fertilization (IVF) using women with simple ovarian cysts as the control group. METHODS: 85 normoresponder patients with endometriomas of 10-50 mm who underwent IVF treatment directly without initial removal were compared ...
Ergenc Ali Fuat - - 2007
ICSI (intracytoplasmic sperm injection) procedure is one of the most commonly used cellular-injection processes. In ICSI a drawn glass pipette is pushed against the biological cell and a series of force impulses are exerted on it axially to achieve the piercing through the zona and the membrane in sequence for ...
Fawole A O - - 2007
The study compared (i) the outcome between cryopreserved ICSI fertilized embryo and cryopreserved ICSI fertilized pronuclear stage zygotes and (ii) the outcome between cryopreserved fertilized mature oocytes with cryopreserved immature oocytes, fertilized by ICSI following thawing. Comparative retrospective review of relevant studies satisfying study criteria. Studies were identified through MEDLINE ...
Wei Zhaolian - - 2008
OBJECTIVE: To determine the effect of metformin pretreatment on in vitro maturation (IVM) in the patients with clomiphene citrate-resistant polycystic ovary syndrome. DESIGN: Prospective randomized study. SETTING: University affiliated hospital. PATIENT(S): Fifty-six women with clomiphene citrate-resistant polycystic ovary syndrome underwent 70 cycles of IVM treatment. INTERVENTION(S): Metformin was administered at ...
Peter L - - 2008
Mutations in the eukaryotic translation initiation factor 2B (eIF2B) represent a heterogenous group of autosomal recessive leucodystrophy characterized by a diffuse CSF-like aspect of the white matter at MRI designed as vanishing white matter (VWM) and episodes of acute deterioration after stresses. The mild juvenile and adult forms are often ...
Shapiro Bruce S - - 2008
Acceptable rates of fertilization, implantation, clinical pregnancy, ongoing pregnancy, and early pregnancy loss were achieved in high responders after triggering final oocyte maturation with a combination of leuprolide acetate and hCG (1,000 to 2,500 IU). These findings, along with the absence of ovarian hyperstimulation syndrome, suggest that this dual trigger ...
Salinas-Flores Liliana - - 2008
The effect of IIF in Pacific oyster oocytes was studied using cryo and transmission electron microscopy (TEM). The viability of oocytes at each step of a published cryopreservation protocol was assessed in an initial experiment. Two major viability losses were identified; one when oocytes were cooled to -35 degrees C ...
Gruber Irmhild - - 2008
In the present study we investigated the effect of a woman's smoking status on the quality of the oocyte, zygote, and on day 3 pre-embryo, as well as the likelihood of achieving an ongoing pregnancy at 8 weeks. Smokers presented a higher number of nonfertilized oocytes than nonsmokers (20.1% vs. ...
Buckett William M - - 2008
OBJECTIVE: To compare rates of pregnancy loss after oocyte maturation in vitro (IVM), after IVF, and after intracytoplasmic sperm injection (ICSI). DESIGN: Retrospective comparative study. SETTING: University tertiary-care center for infertility. PATIENT(S): Women undergoing assisted reproductive technology in a single center. INTERVENTION(S): Oocyte maturation in vitro, IVF, or ICSI, as ...
Sahu Banchhita - - 2008
OBJECTIVE: To compare the oocyte quality and intracytoplasmic sperm injection performance in women with isolated polycystic ovaries or polycystic ovarian syndrome. DESIGN: Retrospective study. SETTING: Fertility unit. POPULATION: Three study groups were identified: women with PCO-only morphology (50 cycles), PCOS (51 cycles) and age matched control group (104 cycles) with ...
Cobo Ana - - 2008
OBJECTIVE: To evaluate the outcome of oocyte vitrification using the Cryotop method, observed in an egg donation program by simultaneously evaluating embryos derived from vitrified and fresh oocytes coming from the same stimulated cycle. DESIGN: Cohort prospective randomized study. SETTING: Instituto Valenciano de Infertilidad (IVI) Valencia, Spain. PATIENT(S): Thirty oocyte ...
Newman Dane M - - 2007
The temporal dynamics of oocyte growth, plasma sex steroids and somatic energy stores were examined during a 12 month ovarian maturation cycle in captive Murray cod Maccullochella peelii peelii under simulated natural photothermal conditions. Ovarian function was found to be relatively uninhibited in captivity, with the exception that post-vitellogenic follicles ...
Lipari Christopher W - - 2008
Final oocyte maturation was achieved by using hCG or two doses of leuprolide acetate in 16 oocyte donors after a GnRH antagonist protocol. Administration of hCG was associated with a statistical trend toward increased L-selectin ligand expression in the endometrium on postretrieval day 5, compared with the leuprolide acetate group.
Duru Namik Kemal - - 2007
OBJECTIVE: To add further data on in vitro fertilization (IVF) outcome and ovarian response after endometrioma stripping via either laparoscopy or laparotomy. STUDY DESIGN: IVF outcome and ovarian response parameters in patients who had undergone unilateral endometrioma stripping at laparoscopy (n=28) or laparotomy (n=10) before IVF were retrospectively compared. RESULTS: ...
Krieg Sacha A - - 2008
OBJECTIVE: To evaluate obstetric complications in women who conceived through donated oocytes compared with women who conceived through assisted reproduction using autologous oocytes. DESIGN: Retrospective cohort analysis. SETTING: Stanford Hospital and Clinics and Lucille Packard Children's hospital, both tertiary referral centers. PATIENT(S): A cohort of 71 oocyte recipients who underwent ...
Banwell K M - - 2007
BACKGROUND: Little is known of how the oxygen environment in the ovarian follicle affects oocyte and embryo development, but this has an important impact on the conditions used for in vitro maturation (IVM) of oocytes. We investigated the effect of varying oxygen concentrations during IVM on subsequent pre and post-implantation ...
Kinzer Donna R - - 2008
OBJECTIVE: To determine the prognosis for clinical pregnancy and delivery after total fertilization failure. DESIGN: Retrospective analysis of patient treatment cycles. SETTING: Private fertility clinic. PATIENT(S): 555 couples who had total fertilization failure during a cycle of conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). INTERVENTION(S): None. MAIN ...
Manjunatha B M - - 2007
The objective was to determine the in vitro developmental competence of buffalo oocytes collected from abattoir-derived ovaries at various stages of the estrous cycle and follicular status. In Experiment 1, ovaries (n=476 pairs) were collected and divided into the following five groups: (a) ovaries with a corpus hemorragicum and no ...
De Geyter Maria - - 2007
The interest in long-term storage of uninseminated oocytes through cryopreservation has seen a recent upsurge, because it provides the potential to assist young women to postpone childbirth after having overcome a malignant disease or delaying childbirth until after management of a professional career. The low fertilisation rate of frozen/thawed oocytes ...
Cobo Ana - - 2007
The current study evaluated how a sudden fall in serum oestradiol during ovarian stimulation in donors affects recipient outcome. After the assessment of pregnancy rate in cases of oestradiol falls of <10 or > or =10% (57.0 versus 45.6%), <20 or > or = 20% (55.2 versus 44.9%), <25 or ...
Engemise Samuel - - 2007
Successful pregnancy in Noonan's syndrome and balanced Robertsonian translocation women is extremely rare. This is because pregnancies in these women usually end in spontaneous miscarriage or termination before 24 weeks gestation due to severe congenital anomaly. In this article, we report the case of a 41-years-old woman with Noonan's syndrome ...
Borini A - - 2007
In the last few years, there has been a significant improvement in oocyte cryopreservation techniques. To investigate the clinical significance of oocyte freezing, an assessment of the cumulative pregnancy rate per started cycle derived from the use of fresh and frozen-thawed oocytes was performed. Between 2004 and 2006, 749 cycles ...
Frattarelli John L - - 2008
OBJECTIVE: To compare embryo and oocyte data between a standard protocol and a luteal phase estradiol protocol. DESIGN: Retrospective paired cohort analysis. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): 60 poor-responder patients undergoing 120 IVF cycles. INTERVENTION(S): Addition of luteal estradiol to the standard IVF protocol. MAIN OUTCOME MEASURE(S): ...
Fauque Patricia - - 2008
OBJECTIVE: To report a case of successful delivery of a healthy baby after intracytoplasmic sperm injection (ICSI) in a patient with no mature oocytes at the time of oocyte retrieval. DESIGN: Case report. SETTING: Department of Reproductive Medicine. PATIENT: After a controlled ovarian hyperstimulation cycle, a total of seven immature ...
McClamrock Howard D - - 2008
There is an increasing body of evidence that excess weight is associated with decreased pregnancy rates, lower birth rates, lower implantation rates, and the possibility of decreased follicle development and oocyte numbers as well as an increased gonadotropin requirement in patients undergoing in vitro fertilization or intracytoplasmic sperm injection. Whether ...
Sugiyama S - - 2007
Previous research by this group (2003) has demonstrated that heat stress during in vitro culture (IVC) significantly increased early embryo mortality. The experiments reported here examine the effects of heat treatment (HT) during in vitro maturation (IVM) and during in vitro fertilization (IVF). One 24 h cycle of HT entailed ...
Khatir H - - 2007
The effect of source of cumulus-oocytes-complexes (COCs), maturation and fertilization conditions on developmental competence of dromedary embryos was examined. Thirty-six adult females were superovulated with equine Chorionic Gonadotropin (eCG) injection (3500 IU, IM) and divided in three groups of 12 females each. Group 1 provided 138 COC's collected from follicles ...
Magnusson Vanessa - - 2008
Success in oocyte cryopreservation is limited and several factors as cryoprotectant type or concentration and stage of oocyte meiotic maturation are involved. The aim of the present study was to evaluate the effect of maturation stage and ethylene glycol (EG) concentration on survival of bovine oocytes after vitrification. In experiment ...
Nishizawa Manabu - - 2007
(11E)-13-Oxo-15,16-dinorlabda-8(20),11-dien-19-oic Acid (1), obtained either from the stem bark of Thuja standishii or readily prepared in larger quantities from the related constituent 2, was found to significantly reduce the formation of papilloma in an in vivo two-stage mouse-skin-carcinogenesis model. Carcinogenesis was initiated by skin exposure to UV-B irradiation and promoted ...
Ireland J J - - 2007
BACKGROUND: The significance of the high variation in numbers of follicles produced during reproductive cycles in humans and cattle is unknown. METHODS: We selected beef heifers with high (> or =25) or low (< or =15) numbers of ovarian follicles and determined the association with alterations in FSH and estradiol ...
Chen Ying - - 2007
In developing mouse ovaries, oocytes develop as clusters of cells called nests or germ cell cysts. Shortly after birth, oocyte nests dissociate and granulosa cells surround individual oocytes forming primordial follicles. At the same time, two thirds of the oocytes die by apoptosis, but the link between oocyte nest breakdown ...
Al-Sunaidi Mohammed - - 2007
OBJECTIVE: To discuss and describe the successful treatment of women with primary infertility using in vitro maturation (IVM) of immature human oocytes resulting in repeated pregnancies and live births in the same individuals. DESIGN: Case report. SETTING: McGill Reproductive Center, Royal Victoria Hospital, McGill University. PATIENT(S): Six patients who underwent ...
Christianson M S - - 2007
BACKGROUND: Use of recombinant follicle stimulating hormone (rFSH) in a cartridge pen device offers obvious benefits for donor oocyte cycles including the administration of fewer and more patient-friendly injectable medications. METHODS: In a University-based IVF program, a total of 98 oocyte donor cycles using rFSH either reconstituted or as a ...
Hashimoto Shu - - 2007
In-vitro maturation (IVM) of immature oocytes has been proposed as a potential alternative to conventional IVF treatment following ovarian stimulation. However, the effects of the oocyte retrieval conditions on subsequent development have not been well understood. This study assessed the effects of different aspiration vacuums during oocyte retrieval on the ...
Schuler A M - - 2007
The objective of this study was to test whether ultrasound-guided oocyte retrieval is an effective mechanism for collecting oocytes in squirrel monkeys. Although ultrasound-guided follicular aspiration has been described in Old World primates, oocyte retrieval in New World primates is typically performed via laparoscopy or laparotomy. However, these procedures, especially ...
Huang Jack Y J - - 2007
Cryopreservation of embryos at the blastocyst stage may provide an effective method to increase the cumulative pregnancy rate for each treatment cycle of ovarian-stimulated IVF. The objective of this study was to evaluate the survival rate and hatching rate of bovine blastocysts following vitrification using a method designed for oocytes, ...
Blickstein Isaac - - 2007
Available hypotheses proposed to explain the mechanism of zygotic splitting fail to explain why monozygotic twins are more prevalent after all methods of assisted reproduction and which structure is likely to control this phenomenon. Arguably, a small proportion of oocytes might have an inborn propensity to undergo splitting upon fertilization ...
Edgar David H - - 2007
Clinical application of oocyte cryopreservation may be in the context of fertility preservation for women about to undergo cytotoxic therapies or may be as an alternative to embryo cryopreservation in routine assisted reproduction. The clinical efficiency of oocyte cryopreservation will be a consequence of the cumulative impact of pre-freeze oocyte ...
Gautam S K - - 2008
The present study was conducted to evaluate the effects of three cryoprotectants, dimethyl sulphoxide (DMSO), ethylene glycol (EG) and 1,2-propanediol (PROH), each used at two concentrations (1.0 and 1.5 M) on the morphology, maturation rate and developmental capacity of usable quality immature buffalo oocytes subjected to slow freezing. The addition ...
Ko Chiu-Sung - - 2008
This study is to investigate the change of morphology of the meiotic spindle and the extent of zona hardening relating to the morphological survival and developmental competence of thawed oocytes. Four- to 8-week-old female mice (C57BL/6) primed with an intraperitoneal injection of pregnant mare's serum gonadotropin and human chorionic gonadotropin. ...
Yoon Tae Ki - - 2007
OBJECTIVE: To report the survival rate of oocytes and the rate of successful pregnancies obtained from super-rapid cooling of oocytes using slush nitrogen (SN(2)). DESIGN: Prospective clinical research. SETTING: A university-affiliated hospital. PATIENT(S): Twenty-eight infertile women who underwent 30 cycles of IVF-ET using previously vitrified oocytes. INTERVENTION(S): Oocytes were vitrified ...
Wu Yan-Ting - - 2007
BACKGROUND: Bone morphogenetic protein-15 (BMP-15) has been shown to influence oocyte maturation and quality. However, no relationship has been established between BMP-15 and oocyte quality/embryonic development in humans. The aim of this study is to investigate BMP-15 level in human follicular fluid (FF) and its possible role in determining oocyte ...
Holzer Hananel - - 2007
OBJECTIVE: To assess the role of immature oocyte collection from unstimulated ovaries as a potential source of oocyte donation. DESIGN: Prospective cohort study. SETTING: A tertiary, university-based, in vitro fertilization center. PATIENT(S): Twelve oocyte donors with ultrasound-only polycystic ovaries or polycystic ovary syndrome matched with 12 oocyte recipients. INTERVENTION(S): Immature ...
Watson A J - - 2007
Efforts have intensified to successfully mature and inseminate oocytes in vitro and then culture ensuing embryos to transferable stages from a large number of mammalian species. Success varies, but generally even for the most successful species it is only possible to obtain a maximum of a 40 to 50% development ...
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