Search Results
Results 101 - 150 of 1426
1 2 3 4 5 6 7 8 9 10 >
Knopman Jaime - - 2010
OBJECTIVE: To characterize incidence, chorionicity, amnionicity, and pregnancy outcome for monozygotic twin pregnancy (MZT) after IVF. DESIGN: Retrospective review. SETTING: University-based fertility center. PATIENT(S): Autologous and oocyte donation IVF cycles eventuating in 4,976 clinical gestations from 2000 to 2007. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): MZT incidence, chorionicity, zygosity, pregnancy outcome. ...
Depalo Raffaella - - 2009
BACKGROUND: The aim of this study was to evaluate the response to treatment in a group of patients undergoing IVF and randomised to receive GnRH-antagonist or the GnRH-agonist. The endpoints were the pattern of follicular growth, the maturity of the oocytes collected, the embryo quality and the pregnancy outcome. METHODS: ...
Kim Sue - - 2009
To improve the efficiency of somatic cell nuclear transfer (SCNT) in dogs, we evaluated whether or not the interval between fusion and activation affects the success rate of SCNT. Oocytes retrieved from outbred dogs were reconstructed with adult somatic cells from a male or female Golden Retriever. In total, 151 ...
Hsieh Ming-Shu - - 2009
Quantum dots (QDs) are useful novel luminescent markers, but their embryonic toxicity is yet to be fully established, particularly in oocyte maturation and sperm fertilization. Earlier experiments by our group show that CdSe-core QDs have cytotoxic effects on mouse blastocysts and are associated with defects in subsequent development. Here, we ...
Magli M Cristina - - 2010
OBJECTIVE: To verify whether the morphologic evaluation of zygotes and embryos derived from thawed oocytes could provide some relevant information regarding their developmental performance. DESIGN: Fertilization, zygote, and embryo morphology from sibling fresh and frozen oocytes was compared. SETTING: Reproductive Medicine Unit, Società Italiana Studi Medicina della Riproduzione, Bologna, Italy. ...
Raine-Fenning Nick - - 2010
OBJECTIVE: To evaluate the effect of a new automated technique of follicle measurement (Sono automated volume calculation [SonoAVC]) on the timing of oocyte maturation and subsequent oocyte retrieval. DESIGN: Prospective randomized controlled trial. SETTING: University-based Assisted Conception Unit. PATIENT(S): Seventy-two women undergoing their first cycle of assisted reproduction treatment. INTERVENTION(S): ...
Zegers-Hochschild Fernando - - 2010
OBJECTIVE: To compare reproductive performance among pregnancies initiated with autologous oocytes and donated oocytes. DESIGN: Retrospective cohort analysis. SETTING: Cl?nica las Condes Hospital, a tertiary referral center in Chile; Fertility, Centro de Fertiliza?ao Assistida, Brazil; and 130 institutions reporting to the Latin American Registry (RLA) of assisted reproductive technologies (ART). ...
Molloy David - - 2009
Cryopreservation of unfertilised oocytes for later use in initiating pregnancy is now a viable technology, with acceptable pregnancy rates (over 20% per thaw cycle). Oocyte cryopreservation used as a form of insurance against "social" (age-related) infertility can improve the lifetime chance of pregnancy in women who defer pregnancy into their ...
Miao De-Qiang - - 2009
Although successful embryo development is dependent upon genetic and epigenetic contributions from both the male and female, the male potential to adversely affect embryo development has been scarcely studied. It is unclear whether the sperm variation among different males would affect the outcome of oocyte evaluation by embryo development following ...
Parmegiani L - - 2009
It has been demonstrated previously that freezing oocytes within 2 h of retrieval increases the efficiency of cryopreservation via a slow-freezing/rapid-thawing protocol with 0.3 mol/l sucrose (SF/RT 0.3). The aim of this multicentre survey was to verify this observation on a larger scale. This was a retrospective study on the ...
Chen Shee-Uan - - 2009
Both the slow-freezing method with increased sucrose concentration and new vitrification techniques significantly improve the results of cryopreservation of human oocytes. The recent perfection for vitrification includes the concepts of increase of cooling and warming rates using minimum volume methods, and decrease of toxicity by reducing the concentration of cryoprotectants. ...
Vlahos Nikos F - - 2009
BACKGROUND: Oocyte retrieval for in vitro fertilization (IVF) is one of the most common minor surgical procedures. OBJECTIVES: To give an update on anesthesia practices used currently in the United States and Europe in assisted reproductive technology, and discuss the safety or the potential risks for oocyte and embryo quality. ...
Kim Thomas J - - 2010
OBJECTIVE: To determine the efficiency of our vitrification technique when applied in young fertile women. DESIGN: Clinical research and application. SETTING: In vitro fertilization center. PATIENT(S): Twenty-one women were recruited from the navy community with 19 patients finishing the study. INTERVENTION(S): Vitrified oocytes with use of the electron microscopic grid ...
Jirsov? Simona - - 2010
OBJECTIVE: To establish the effect of chlorinated organic compounds on fertility. DESIGN: We analyzed the influence of polychlorinated biphenyls (PCBs) and pesticides in follicular fluid on the number of fertilized oocytes, the quality of oocytes and embryos, pregnancy rate, and number of embryos cryoconserved in IVF-ET program. SETTING: Department of ...
Bechoua S - - 2009
The successful outcome of intracytoplasmic sperm injection (ICSI) with globozoospermic sperm and non-activated oocytes is reported. Three couples underwent ICSI treatment and two of the patients were siblings. Forty-four non-activated oocytes were injected, 26 oocytes fertilised normally and 17 good quality embryos were obtained. Six embryo transfers were carried out, ...
Petersen C G - - 2009
The objective of this meta-analysis was to investigate the influence of meiotic spindle visualization in human oocytes on intracytoplasmic sperm injection (ICSI) outcomes. Search strategies included on-line surveys of databases (MEDLINE, EMBASE, Science Citation Index, Cochrane Controlled Trials Register and Ovid). The fixed effect was used for odds ratio. Ten ...
Erb Teresa M - - 2010
To compare leuprolide acetate to hCG as the trigger for final oocyte maturation in oocyte donor cycles. Retrospective review. Academic IVF donor program. Thirty-two healthy oocyte donors aged 21-33 years with adequate ovarian reserve. Donors were down-regulated with cetrorelix and received either leuprolide acetate (n = 12) or hCG (n ...
Benkhalifa M - - 2009
In-vitro maturation (IVM) was performed in 350 cycles for 262 unstimulated patients diagnosed with polycystic ovary syndrome who were primed with human chorionic gonadotrophin (HCG) before oocyte retrieval. In order to improve nuclear and cytoplasmic maturation, growth hormone was added to the maturation medium. Oocytes were recovered in 94.8% of ...
Check J H - - 2009
PURPOSE: The need for freezing oocytes has been established for females undergoing potential therapy that could damage their ovarian egg reserve, for ethical or religious reasons (not having excess embryos frozen) or for women nearing the age of lower fecundity but not married and not ready to use donor sperm. ...
- - 2009
AIM: The aim of this article is to review the evidence regarding human oocyte cryopreservation by slow freezing and vitrification and to provide evidence-based clinical and laboratory guidelines on the effectiveness and safety of these technologies. SCOPE: These guidelines will address the following: Stage of oocyte maturity. Methods of cryopreservation/warming. ...
Check J H - - 2009
PURPOSE: To describe a unique case of a successful gestational carrier pregnancy in a woman with premature ovarian failure using her own oocyte. METHODS: Despite amenorrhea, failure to have menses to progesterone withdrawal and resistance to gonadotropin stimulation, ovulation induction was attempted by restoring down-regulated follicle stimulating hormone (FSH) receptors ...
Selvaraj Priya - - 2009
We report the first pregnancy and birth in India after the transfer of embryos generated from frozen- thawed oocytes. A 29-year-old woman with previous bad obstetric history and an abnormal karyotype, necessitating donor oocyte programme. Embryos were generated by microinjection of frozen-thawed sperms into thawed human oocytes (intracytoplasmic sperm injection). ...
Galindo Anna - - 2009
To compare donor and recipient outcome after inducing the final oocyte maturation with hCG or GnRH agonist in GnRH-antagonist treated oocyte donation (OD) cycles. Two-hundred fifty-seven oocyte donors were enrolled to participate in a clinical trial in a private fertility centre. After stimulation with 225 IU rFSH and Cetrorelix 0.25 ...
Revelli Alberto - - 2009
The assessment of oocyte quality in human in vitro fertilization (IVF) is getting increasing attention from embryologists. Oocyte selection and the identification of the best oocytes, in fact, would help to limit embryo overproduction and to improve the results of oocyte cryostorage programs. Follicular fluid (FF) is easily available during ...
Ou Yu-Che - - 2010
OBJECTIVE: To compare the results of IVF and intracytoplasmic sperm injection (ICSI) in extremely low oocyte retrieval cycles. DESIGN: Case-control study. SETTING: An IVF laboratory in a university hospital in Taiwan. PATIENT(S): Between January 2003 and December 2007, infertile couples with one, two, or three oocytes per retrieval were divided ...
Ezcurra Diego - - 2008
The Human Oocyte Preservation Experience (HOPE) Registry is an initiative of EMD Serono which aims to systematically track the outcomes of oocyte cryopreservation cycles and validate the efficacy and safety of techniques to freeze and thaw oocytes. Beginning in November 2008 (ASRM, San Francisco, CA), the registry will be performed ...
Riggs Ryan - - 2010
OBJECTIVE: To evaluate the impact of cryopreservation storage duration on embryo survival, implantation competence, and pregnancy outcome. DESIGN: Retrospective study. SETTING: Academic tertiary-referral infertility center. PATIENT(S): In vitro fertilization patients and recipients of oocyte donation cycles who had cryopreserved embryos and underwent at least one thaw cycle from 1986 to ...
Fan Zhi Qiang - - 2009
Cryopreservation of mouse spermatozoa has been widely used; however, fertility of frozen spermatozoa in some strains, especially when inseminating cryopreserved oocytes, is low and may be improved by assisted fertilization techniques. The present study was performed to investigate the effect of partial zona pellucida (ZP) digestion on the in vitro ...
Heindryckx Björn - - 2008
Failed fertilization after intracytoplasmic sperm injection (ICSI) can occur due to an oocyte activation defect. In these cases assisted oocyte activation (AOA) may help but efficiency is still unknown. Prior to AOA, the mouse oocyte activation test (MOAT) can be carried out by injecting human spermatozoa into mouse oocytes to ...
Chang Ching-Chien - - 2008
This study aimed to evaluate oocyte vitrification efficiency using in-vivo matured (IVO) versus rescued in-vitro matured (IVM) oocytes. The results show that oocyte survival (85% versus 81%), fertilization (86% versus 76%) and cleavage rate (98% versus 89%) was not significantly different in IVO oocytes compared with rescued IVM sibling oocytes. ...
Feugier Alexandre - - 2008
Gametes and embryo tissues are known to represent a sensitive target to environmental toxicants exposure. Oocyte quality can impact subsequent developmental competence, pregnancy course and even adult health. The major health concern from depleted uranium (DU) is mainly centred on its chemotoxic properties as a heavy metal. Little attention was ...
Reisaeter A V - - 2009
Reports on pregnancies in kidney donors are scarce. The aim was to assess pregnancy outcomes for previous donors nationwide. The Medical Birth Registry of Norway holds records of births since 1967. Linkage with the Norwegian Renal Registry provided data on pregnancies of kidney donors 1967-2002. A random sample from the ...
Sher G - - 2008
Enthusiasm for oocyte cryopreservation has been limited by poor pregnancy rates per thawed metaphase II (MII) oocytes (<4%) and low implantation rates per embryos. The reasons relate to technical limitations in the freezing process, and the fact that <40% of oocytes are euploid and unable to produce 'competent' embryos. Comparative ...
Ding Jianchi - - 2008
BACKGROUND: The technology for successful oocyte cryopreservation has evolved only over the past decade. Fewer than 300 pregnancies achieved with cryopreserved oocytes were reported as of the end of 2006. Two cryopreservation techniques are currently available: the slow-cooling method and vitrification. This article reviews the world literature on pregnancies conceived ...
Albani E - - 2008
Over the last few years, there has been renewed interest and scientific debate concerning human oocyte cryopreservation. The aim of this study was to analyse the clinical data coming from our long experience of slow-freezing oocytes. Between 2001 and 2007, 1280 thawing cycles were carried out using oocytes previously frozen ...
Greco E - - 2008
Italian legislation regarding reproductive medicine prohibits embryo storage while allowing cryopreservation of supernumerary oocytes. This study evaluated the effect of fresh oocytes obtained from natural unstimulated cycles on the clinical success rates derived from the use of frozen-thawed (FR-TH) oocytes obtained following ovarian stimulation. For 36 women, intracytoplasmic sperm injection ...
Oyesanya Olufunso A - - 2009
OBJECTIVE: To prospectively compare the prognosis of IVF-ET cycles using oocyte sharing vs. cycles using altruistic donors. DESIGN: Prospective cohort prognostic study. SETTING: University teaching hospital. PATIENT(S): A total of 353 consecutive infertile women with premature ovarian failure or diminished ovarian function. INTERVENTION(S): After receipt of institutional ethics approval, IVF-ET ...
Nakagawa Shoma - - 2008
In this study, we examined the effects of inhibitors of mitochondrial permeability transition (MPT), caspase activity, intracellular Ca(2+) chelator and mitochondrial Ca(2+) uniporter on survival assessed by morphological observation and in vitro maturation (IVM) of porcine vitrified germinal vesicle (GV) oocytes. When vitrified GV oocytes were matured only present in ...
Varga Erika - - 2008
The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 ...
Verwoerd Gideon R - - 2008
Ovarian hyperstimulation syndrome (OHSS) is a rare but potentially fatal complication of IVF treatment. The risk of OHSS increases with increasing numbers of follicles aspirated and oocytes retrieved, but there is little evidence to support whether threshold values of either can be used to correctly predict OHSS. Since the most ...
Nyachieo Atunga - - 2009
The aim of this review paper is to provide a scientific basis for the development of ovarian stimulation (OS) protocols for in vitro fertilization (IVF) in baboons. Firstly, the evidence available regarding OS for assisted reproduction in baboons is reviewed based on available published data, assessed by a Pub Med ...
Manno Massimo - - 2009
At present, there is no agreement on poor ovarian response definition, and no definitive evidence that this prognosis can be changed by a specific protocol. Our data suggest that a flare-up protocol with a depot gonadotropin-releasing hormone (GnRH) agonist formulation gives higher total pregnancy and implantation rates than a GnRH ...
Nagy Zsolt P - - 2009
OBJECTIVE: To evaluate the efficiency of oocyte donation cycles using egg "cryo-banking." DESIGN: Study conditions for vitrified/warmed oocytes for 20 non-autologous recipients (from 10 donors) were set prospectively, and outcomes of it were later compared retrospectively to nine fresh donations cycles. SETTING: Private assisted reproductive technology program. PATIENT(S): Ten donors ...
Abir Ronit - - 2009
OBJECTIVE: To compare the development of human fetal follicles from late-gestational-age fetuses frozen-thawed gradually and slowly with dimethylsulfoxide (DMSO) or 1,2 propanediol (PROH) and sucrose after renal grafting into follicle stimulating hormone-treated immunodeficient mice. DESIGN: Controlled histologic study of grafted human fetal ovaries. SETTING: Major tertiary care academic center. PATIENT(S): ...
Plante Beth J - - 2008
OBJECTIVE: To report a successful pregnancy in a patient with pure 46,XY gonadal dysgenesis. DESIGN: Case report. SETTING: Academic reproductive endocrinology and infertility unit. PATIENT(S): A patient with pure 46,XY gonadal dysgenesis and a desire to become pregnant. INTERVENTION(S): Laparoscopic gonadectomy, in vitro fertilization using donor oocytes, transfer of cryopreserved ...
Agarwal Ashok - - 2008
Physiological levels of reactive oxygen species (ROS) play an important regulatory role through various signaling transduction pathways in folliculogenesis, oocyte maturation, endometrial cycle, luteolysis, implantation, embryogenesis, and pregnancy. Persistent and elevated generation of ROS leads to a disturbance of redox potential that in turn causes oxidative stress (OS). Our literature ...
Alm H - - 2008
Holding immature oocytes before maturation simplifies the transport of oocytes and aids in scheduling later manipulations. We examined the effect of holding bovine oocytes in the absence of meiotic inhibitors on their subsequent meiotic and developmental competence. Oocytes were matured immediately after recovery (control) or were held in a mixture ...
Mori Hironori - - 2008
The present study was carried out to examine the parthenogenetic development of pig oocytes treated with different concentrations of cycloheximide for different durations following activation by ultrasound stimulation. When oocytes were treated with 10 microg/ml cycloheximide for different durations, the blastocyst formation rate of oocytes treated for 5 h was ...
Menkhorst Ellen - - 2008
Extracellular egg coats deposited by maternal or embryonic tissues surround all vertebrate conceptuses during early development. In oviparous species, the time of hatching from extracellular coats can be considered equivalent to the time of birth in viviparous species. Extracellular coats must be lost during gestation for implantation and placentation to ...
Robker Rebecca L - - 2008
Infertility is more common in overweight and obese women, with reproductive impairments occurring at many levels of the hypothalamic-ovarian-uterine axis. These impairments lead primarily to longer times to conception and decreased pregnancy rates and have resulted in increasing numbers of overweight and obese women seeking assisted reproduction technologies, such as ...
1 2 3 4 5 6 7 8 9 10 >