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Smith Gary D - - 2010
To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. Prospective randomized. Academically affiliated, private fertility center. Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were randomized to slow-rate freezing or ...
Stoop D - - 2010
BACKGROUND: The prevention of multiple pregnancies remains a major challenge in patients treated with ovarian stimulation prior to intrauterine insemination (IUI). The pilot study presented here investigates whether multiple pregnancies can be minimized by a microscopically confirmed aspiration of oocytes from supernumerary follicles immediately before intrauterine insemination and evaluates the ...
Laanpere Margit - - 2010
This review summarizes current knowledge of the effect of folate-mediated one-carbon metabolism and related genetic variants on female fertility and pregnancy viability. Insufficient folate status disrupts DNA methylation and integrity and increases blood homocysteine levels. Elevated levels of follicular fluid homocysteine correlate with oocyte immaturity and poor early embryo quality, ...
Zhao Jun-Zhao JZ The First Affiliated Hospital of Wenzhou Medical College, Zhejiang, People's Republic of China. - - 2010
Abstract Objective: To evaluate the effects of oral contraceptives and metformin on the outcome of in vitro maturation (IVM) in infertile women with polycystic ovary syndrome (PCOS). This is a retrospective study of 108 women with PCOS, subject to 152 cycles of IVM treatment. The study was held at the ...
Knopman Jaime M - - 2010
OBJECTIVE: To report the use of previously cryopreserved oocytes for the treatment of secondary infertility. DESIGN: Case report. SETTING: University-based IVF program. PATIENT(S): A 41-year-old woman with 18 months of secondary infertility and a previous history (age 38) of elective oocyte cryopreservation. INTERVENTION(S): Previously cryopreserved oocytes. MAIN OUTCOME MEASURE(S): Fertilization, ...
DiLuigi Andrea J - - 2010
Ninety-four women undergoing IVF with peak E2 level>4000 pg/mL received leuprolide acetate (LA) trigger (LA trigger group) or had gonadotropins withheld for one or more days (coasting group) followed by hCG trigger, unless cycle cancellation occurred. There were no cases of ovarian hyperstimulation syndrome in either group, and the LA ...
Huddleston Heather G - - 2010
To determine if differences exist in ovarian response and pregnancy rates between Asian and Caucasian donors. Retrospective cohort study. University-based clinic. Anonymous oocyte donors of self-reported Asian (n=63) or Caucasian (n=156) ethnicity who began ovarian stimulation between February 2000 and March 2008 and their matched recipients. None. Recipient pregnancy rates ...
Borini Andrea - - 2010
OBJECTIVE: To evaluate the efficacy of oocyte cryopreservation by a single slow-cooling protocol involving sucrose (0.2 mol/L) in the freezing solution. DESIGN: Observational comparison of the clinical outcome in fresh and frozen thawed cycles. SETTING: Public and private IVF centers. PATIENT(S): Infertile couples undergoing IVF treatment. INTERVENTION(S): Use of a ...
Bertoldo M - - 2010
The modern domestic sow exhibits a period of impaired reproductive performance during the late summer and early autumn months, known as 'seasonal infertility'. A reduction in farrowing rate due to pregnancy loss is the most economically important manifestation of seasonal infertility. The aim of the present study was to determine ...
Chen Juan - - 2010
Fertilisation failures occur in 2-3% of intracytoplasmic sperm injection (ICSI) cycles and are mostly caused by failure of oocyte activation. Assisted oocyte activation (AOA) may be an efficient treatment option to overcome oocyte activation failure. To evaluate the effectiveness of ICSI combined with AOA by strontium, six couples with complete ...
Jee Byung Chul - - 2010
BACKGROUND/AIM: The aim of this meta-analysis was to evaluate whether IV albumin infusion around the time of oocyte retrieval prevents ovarian hyperstimulation syndrome (OHSS) and affects the pregnancy rate. METHODS: A meta-analysis of data from 9 randomized controlled trials including 1,613 women at high risk for developing OHSS in in ...
Giulini Simone - - 2010
We report a case of right adnexal torsion during pregnancy after an oocyte in vitro maturation and intracitoplasmic sperm injection cycle in patient with polycystic ovary syndrome. A 31-year-old woman with a typical clinical disorder of polycystic ovarian syndrome was included in an oocyte in vitro maturation program. Right adnexal ...
Sills Eric Scott - - 2010
Premature ovarian failure (POF) remains a clinically challenging entity because in vitro fertilisation (IVF) with donor oocytes is currently the only treatment known to be effective. A 33 year-old nulligravid patient with a normal karyotype was diagnosed with POF; she had a history of failed fertility treatments and had an ...
Selvaraj Priya - - 2010
We report the first twin pregnancy and birth in India after the transfer of embryos generated from vitrified and thawed oocytes to a 41-year-old woman who was in a donor program. Embryos were generated from the microinjection of pre-prepared sperms into vitrified oocytes. Twin male babies weighing 750 and 860 ...
Glujovsky Demián - - 2010
If a fresh embryo, assisted reproductive technology procedure cycle is unsuccessful and there are frozen embryos available, a frozen-thawed embryo transfer is performed. In some specific cases women may undergo oocyte donation treatment. In both situations the endometrium is primed by the administration of estrogen and progesterone. To prevent the ...
Wongtra-Ngan Supreeya - - 2010
Ultrasound guided transvaginal aspiration of oocytes has replaced other methods of oocyte retrieval for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). However, there is controversy over whether flushing yields a larger number of oocytes and a higher potential for pregnancy than aspiration only. To determine whether follicular aspiration ...
Dowling-Lacey Donna - - 2010
The objective was to report two singleton live births after transfer of cryopreserved-thawed day-3 embryos resulting from an unstimulated in-vitro oocyte maturation (IVM) cycle. A 29-year-old female patient with polycystic ovaries (PCO) underwent an unstimulated IVM cycle. A total of 43 prophase-I oocytes were retrieved; 21 oocytes achieved in-vitro maturation ...
Kunz Georg - - 2010
PURPOSE: The uterine junctional zone as documented by magnetic resonance imaging (MRI) has shown to be of significance with respect to the early process of human reproduction. In this study, MRI findings were related to the outcome of an IVF/ICSI program. MATERIALS AND METHODS: In 113 infertile women, the diameter ...
Van Den Bergh Marc Jg - - 2009
The aim of this study was to determine whether the early stages of embryo development, as assessed by the zygote score (Z-score), could be influenced by the injection of spermatozoa that had been preselected on the basis of their binding to hyaluronic acid (HA). A total of 407 sibling metaphase ...
Hassold Terry T School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman, Washington 99164, USA. - - 2009
The relationship between increasing maternal age and trisomy has been recognized for over 50 years and is one of the most important etiological factors associated with any human genetic disorder. Specifically, the risk of trisomy in a clinically recognized pregnancy rises from about 2-3% for women in their twenties to ...
Ashworth Cheryl J - - 2009
The environment in which a breeding female lives prior to conception and during the early stages of her pregnancy has striking effects on oocytes developing in the ovarian follicle and on early embryos in the reproductive tract. Of the various environmental factors known to affect oocyte and embryo development, altered ...
Bodri Daniel - - 2010
BACKGROUND: Race and ethnicity are one of the newly investigated patient-related prognostic factors that might affect the outcome of assisted reproduction techniques. To our knowledge no data currently are available on the effect of race on oocyte donation outcome. MATERIALS: A retrospective, matched cohort study was performed in a private ...
Younis Johnny S - - 2009
To gain insight into the morphology of the first polar body (1 PB) in ICSI patients and to explore whether it could predict mature oocyte viability and performance in this setting. Seventy two consecutive women planned to perform ICSI treatment were prospectively recruited for this study. All oocytes retrieved underwent ...
Rienzi Laura L G.E.N.E.R.A. Centre for Reproductive Medicine, Clinica Valle Giulia, via de Notaris 2B, Rome, Italy. - - 2010
A successful oocyte cryopreservation programme is of utmost importance where a limited number of oocytes can be inseminated per cycle, to overcome legal and ethical issues related to embryo storage, for oocyte donation programmes and for fertility preservation (especially for cancer patients). Vitrification has been recently proposed as an effective ...
Barton Sara E - - 2010
OBJECTIVE: To determine whether oocyte donor FSH and age are independently associated IVF cycle success. DESIGN: Retrospective cohort study. SETTING: University hospital-based IVF clinic. PATIENT(S): Three hundred twelve donor/recipient pairs undergoing oocyte donation IVF. MAIN OUTCOME MEASURE(S): Number of mature oocytes and embryos, clinical pregnancy, and live birth rates. RESULT(S): ...
Mullin Christine M - - 2010
OBJECTIVE: To determine whether there is a difference in pregnancy outcomes between women undergoing a shared versus exclusive donor oocyte cycle. DESIGN: Retrospective analysis. SETTING: University IVF center. PATIENT(S): Women undergoing either a shared (n=656 cycles), exclusive (n=225 cycles), or shared converted to exclusive (n=22 cycles) donor oocyte cycle from ...
Nagy Zsolt Peter - - 2009
Vitrification is now a widely applied and highly successful approach for cryopreservation in reproductive biology. Rapidly increasing data prove that it is also a highly efficient technique for low-temperature storage of human oocytes. The latest approaches with appropriately selected cryoprotectants, tools and techniques, and properly adjusted parameters allow close to ...
Cao Yun-Xia - - 2009
The development of an effective oocyte-freezing program will have a major impact on clinical practice in reproductive medicine and will serve as a powerful tool to preserve fertility for teenage girls and young women without male partners or for those individuals who are affected by malignancies. It will also be ...
Egashira Akiyoshi - - 2009
OBJECTIVE: To present the effectiveness of intracytoplasmic sperm injection (ICSI) using globozoospermic sperm and assisted oocyte activation by electrical stimulation. DESIGN: A case report. SETTING: A private IVF center in Japan. PATIENT(S): A man with globozoospermia. INTERVENTION(S): Acridine orange (AO) test, mouse oocyte activation test, and ICSI with electrical oocyte ...
Hamel M?lanie - - 2010
Embryo selection efficiency in human IVF procedure is still suboptimal as shown by low pregnancy rates with single embryo transfer (SET). Bidirectional communication between the oocyte and follicular cells (FC) is essential to achieve developmental competence of the oocyte. Differences in the gene expression profile of FCs from follicles leading ...
Yoldemir Tevfik - - 2010
OBJECTIVE: To determine if women with less than five eggs retrieved do as well as women with more eggs retrieved in terms of pregnancy outcomes in our assisted reproduction program. METHOD: Two hundred one women undergoing in vitro fertilization treatment at Fertility Unit in Royal Prince Alfred Hospital, University of ...
DeUgarte Daniel A - - 2010
In a retrospective cohort review of third-party reproduction, we observed that surrogate body mass index (BMI) negatively impacts implantation rates in oocyte-donor in vitro fertilization cycles. A BMI > or =35 kg/m(2) cutoff is associated with a statistically significant decrease in pregnancy rates but not miscarriage rates.
Bianchi Veronica - - 2009
OBJECTIVE: To present a case where recombinant human FSH (r-hFSH) was accidentally used instead of hCG and resulted in oocyte maturation. DESIGN: Case report. SETTING: A tertiary infertility center. PATIENT(S): A 36-year-old woman underwent IVF for infertility problems. The couple was unable to obtain a pregnancy during the previous 2 ...
Hermes R - - 2009
Numerous reports on reproductive pathology in all rhinoceros species illustrate the abundance of female infertility in captive populations. In infertile rhinoceroses, oocyte collection and embryo production could represent the best remaining option for these animals to reproduce and to contribute to the genetic pool. We report here on superstimulation, repeated ...
Shapiro Bruce S - - 2010
This retrospective cohort study compared blastocyst transfers in 136 fresh oocyte donor cycles and 69 autologous cycles using blastocysts derived from culture of thawed bipronuclear oocytes, all with oocytes derived from patients or donors less than 35 years old. The autologous cycles and oocyte donor cycles had similar rates of ...
Ferraretti Anna P - - 2010
OBJECTIVE: To identify factors that might affect the clinical outcome of oocyte slow freezing. DESIGN: Retrospective study. SETTING: Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy. PATIENT(S): Patients with spare metaphase II cryopreserved oocytes performing 371 thawing cycles. INTERVENTION(S): Oocytes were cryopreserved by slow freezing<40 ...
Dolmans Marie-Madeleine - - 2009
BACKGROUND: Chemo- or radiotherapy can induce premature ovarian failure (POF), and ovarian tissue cryopreservation and transplantation may be proposed to restore ovarian function. Our aim was to evaluate the quality of oocytes and embryos derived from frozen-thawed transplanted ovarian tissue. MATERIALS AND METHODS: Women were 21-28 years old at tissue ...
Liow Swee Lian - - 2009
A couple with male infertility due to non-obstructive azoospermia were referred to the fertility centre for treatment. Testicular biopsy was performed on the male partner and testicular samples were frozen. The female partner underwent ovarian stimulation and 31 mature oocytes were recovered by ultrasound-guided vaginal aspiration. Twelve oocytes were cryopreserved ...
Fadini R - - 2009
The success of reproductive technologies is facilitated by the cryopreservation of embryos and gametes. In Italy, where legislation prohibits zygote and embryo cryopreservation, clinics have extensively introduced oocyte cryopreservation. Two different strategies of oocyte cryopreservation are available: slow freezing or ultrarapid cooling (vitrification). Although the results are very encouraging with ...
Kadoch Isaac Jacques - - 2009
The recovery of a mature oocyte from a modified natural cycle followed by in-vitro fertilization (nIVF) is an attractive alternative to conventional IVF, involving ovarian stimulation, in the treatment of female infertility. Ovarian agenesis is a rare disorder resulting in primary amenorrhoea and infertility in affected females. A couple sought ...
Kyono Koichi - - 2009
To report a successful delivery of a healthy baby after transfer of vitrified-warmed blastocysts derived from introcytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm. A female patient and her husband with non-obstructive azoospermia received a transfer of vitrified-warmed blastocysts from vitrified-warmed oocytes and frozen-thawed sperm. The main outcome ...
Akarsu Cem - - 2009
OBJECTIVE: To document the relationship between smooth endoplasmic reticulum (SER) aggregations and recurrent fetal anomalies. DESIGN: Case report. SETTING: Private IVF center. PATIENT(S): A 28-year-old woman with an 11-year history of primary infertility. INTERVENTION(S): Three consecutive cycles of intracytoplasmic sperm injection (ICSI) in the same patient. MAIN OUTCOME MEASURE(S): Clinical ...
Hourvitz Ariel - - 2010
OBJECTIVE: To investigate the efficacy of in vitro oocyte maturation (IVM) in women with repeated failure of IVF treatments due to follicular developmental abnormalities. DESIGN: Prospective longitudinal study. SETTING: The IVF unit of a university-affiliated medical center. PATIENT(S): Seven women with three or more IVF failures due to abnormal oocyte ...
Vlahos Nikos F - - 2009
OBJECTIVE: To present a case of aberrant follicular development, acquisition of mature oocytes, and pregnancy after 2 weeks of leuprolide acetate administration during the midluteal phase. DESIGN: Case report. SETTING: Department of Obstetrics and Gynecology, Aretaieio University Hospital, Athens, Greece. PATIENT(S): A 37-year-old white woman who was scheduled to undergo ...
Makrakis Evangelos - - 2009
A 26-year old patient with a mosaic (45X/46XX) Turner's syndrome and secondary amenorrhea since the age of 19, was referred to the IVF unit for participation in oocyte donation program. After a complete work up including meticulous cardiological assessment, she had endometrial preparation with a high dose hormonal regimen, and ...
Zeyneloglu Hulusi Bulent - - 2009
PURPOSE OF REVIEW: To evaluate the current place of cocultures in in-vitro fertilization. The cocultures may have a role in a very special group of patients who had incompetent embryos grown in the conventional media. RECENT FINDINGS: There is evidence that cocultures help embryos to grow better; however, there is ...
Tao Tao - - 2009
PURPOSE OF REVIEW: This review summarized the clinical breakthroughs in the human oocyte cryopreservation field in the past 2 years and gave special emphasis on the role of vitrification method. Human oocyte cryopreservation is an attractive strategy to preserve female fertility, as it offers more opportunities to the future destination ...
Tamer Erel Cemal - - 2009
PURPOSE OF REVIEW: Obesity is a worldwide problem. The effect of obesity on the outcome of assisted reproductive techniques is quite controversial. RECENT FINDINGS: Although there is not an absolute consensus based on evidence, obesity may lengthen the duration of ovulation induction, increase the gonadotrophin dose, decrease the peak estradiol ...
Parmegiani L - - 2009
The potential, limits and safety of oocyte freezing are still being explored. Female age may play a relevant role in treatment outcome. The present study is the first report of the birth and normal development of a baby conceived from frozen oocytes of a 40-year-old woman. IVF was carried out ...
Macas Ervin - - 2009
This report describes the first successful case of preimplantation genetic diagnosis (PGD) for myotonic dystrophy type Curschmann-Steinert (DM1) using polar body biopsy with vitrification. A 39-year-old woman with expansion of a CTG trinucleotide repeat in the DMPK gene was included into the study centre's PGD programme. After intracytoplasmic sperm injection, ...
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