Search Results
Results 301 - 350 of 1965
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Li Q Y - - 2008
The aim of this experiment was to examine whether a simplified mini-laparotomy technique is suitable for embryo transfer in ewes. In vitro produced blastocysts were transferred to the uterine horns of synchronous recipient ewes. Each recipient received 1 embryo by conventional laparotomy (n = 36), laparoscopy (n = 21), or ...
Kattal Namita - - 2008
OBJECTIVE: To evaluate the role of coculture in human IVF. DESIGN: Meta-analysis. SETTING/PATIENT(S)/INTERVENTION(S): A literature search was performed using the Cochrane Menstrual Disorders and Subfertility Group Trials register, the Cochrane Central register of Controlled Trials on the Cochrane Library (2006), and MEDLINE (January 1966 to March 2006). MAIN OUTCOME MEASURE(S): ...
Bedaiwy Mohamed A - - 2008
OBJECTIVE: The objective of this study was to compare reproductive function after two neurosurgical procedures for treating non-neoplastic hydrocephalus; endoscopic third ventriculostomy (ETV) and ventriculo-peritoneal shunt (VP). STUDY DESIGN: A cohort of 96 women who underwent neurosurgical procedures to treat non-neoplastic hydrocephalus at the Cleveland Clinic between January 1995 and ...
Porter Misty Blanchette - - 2008
Transvaginal ultrasound-guided oocyte retrieval is the gold standard for in vitro fertilization (IVF) treatment. Despite its relative safety, oocyte retrieval is associated with risk to the adjacent pelvic organs, bleeding, and pelvic infection. The embryo transfer (ET) procedure is considered a crucial step in an IVF cycle. The success of ...
Bhatt Taimur - - 2008
BACKGROUND: In vitro fertilization (IVF) with single embryo transfer (SET) has been proposed as a means of reducing multiple pregnancies associated with infertility treatment. All existing cost-effectiveness studies of IVF-SET have compared it with IVF with multiple embryo transfer but not with intrauterine insemination with gonadotropin stimulation (sIUI). METHODS: We ...
McArthur S J - - 2008
OBJECTIVE: Modern in vitro fertilization practices involve transfer of embryos as blastocysts, when anabolic metabolism is well established and pregnancy rates can be maintained while transferring embryos singly to avoid multiple pregnancies. Embryo biopsy for preimplantation genetic diagnosis (PGD), however, is generally performed on day 3, when the embryo comprises ...
Vlaisavljevic Veljko - - 2008
As multiple pregnancy is now considered to be an adverse event of an IVF procedure, reducing the multiple pregnancy rates has become the goal of many IVF centres. A large number of double blastocyst transfers in non-selected patients were performed in the author's institution over recent years, and a retrospective ...
Souza Maria do Carmo B Mdo - - 2009
OBJECTIVE: To evaluate the impact of two different incubation environments-class 8 versus class 5-on embryo quality and pregnancy rate. DESIGN: Retrospective comparative study. SETTING: Private fertility and gynecology clinic. PATIENT(S): 123 consecutive intracytoplasmic sperm injection (ICSI) cycles were analyzed from January 2002 to February 2005. Cycles were divided into two ...
Berkes Eniko - - 2008
OBJECTIVE: To present a case report of a unilateral triplet ectopic pregnancy (EP) conceived by IVF-embryo transfer. DESIGN: Case report. SETTING: University Hospital, Budapest, Hungary. PATIENT(S): A 26-year-old infertile woman with a history of right salpingectomy, hyperprolactinemia, and male factor infertility underwent IVF-embryo transfer of three embryos. Early transvaginal sonography ...
Shih W - - 2008
BACKGROUND: Data show that differences exist in the birthweight of singletons after frozen embryo transfer (FET) compared with fresh transfer or gamete intra-Fallopian transfer (GIFT). Factors associated with low birthweight (LBW) after assisted reproduction technology (ART) were studied. METHODS: Birthweight, distribution of birthweight, z-score, LBW (<2500 g), gestation and percentage ...
Eskandar Mamdoh - - 2008
OBJECTIVE: To determine whether the implementation of ultrasound (US) guidance will improve the clinical outcomes of ET compared with the standard clinical touch method of embryo catheter placement. DESIGN: Prospective, single-operator, randomized, controlled trial. SETTING: Saudi Center for Assisted Reproduction. PATIENT(S): Three hundred seventy-three women. INTERVENTION(S): Transcervical, intrauterine ET with ...
Martinuzzi Kurt - - 2008
To determine if elevated body mass index in young women with normal ovarian reserve was associated with poorer ovarian response, difficulty at embryo transfer, and lower clinical pregnancy rates. Retrospective study of 417 first, fresh in vitro fertilization cycles performed between October 2004 and December 2006. All women were under ...
Lathi Ruth Bunker - - 2009
To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst. Techniques and instrumentation. A University Medical Center. Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program. Frozen blastocysts ...
Esfandiari Navid - - 2008
OBJECTIVE: To report a case of a quintuplet pregnancy following transfer of five poor-quality cellular-stage embryos. DESIGN: Case report. SETTING: University of Toronto-affiliated infertility clinic. PATIENT(S): A 35-year-old female with a 2-year history of secondary infertility. INTERVENTION(S): Intracytoplasmic sperm injection and embryo transfer. MAIN OUTCOME MEASURE(S): Clinical pregnancy. RESULT(S): Quintuplet ...
Gunby Joanne - - 2009
OBJECTIVE: To present a report on assisted reproductive technologies (ART) cycles performed in 2005 in Canada. This is the fifth annual report from the Canadian ART Register (CARTR). DESIGN: Prospective cohort study. SETTING: Twenty-five of 25 ART centers in Canada. PARTICIPANT(S): Couples undergoing ART treatment in Canada during 2005. INTERVENTION(S): ...
Beesley Ronald - - 2009
OBJECTIVE: To test the hypothesis that day 5 ET (D5ET) is superior to day 3 ET (D3ET) in pregnancy outcome and that it also reduces multiple gestations. DESIGN: Retrospective cohort study. SETTING: Assisted reproductive technologies program at Wilford Hall Medical Center. PATIENT(S): Patients electing for either D3ET or D5ET. INTERVENTION(S): ...
Vercammen M J - - 2008
BACKGROUND: There have been concerns about validity and accuracy of the measurement of sHLA-G in embryo culture supernatants. In this systematic review, we quantified the diagnostic accuracy of sHLA-G for predicting the ability to achieve clinical pregnancy in women who are undergoing infertility treatment. METHODS: Medline and Embase were searched ...
Shokeir Tarek - - 2009
We evaluated the uterine cavity by sonohysterography (SHG) in patients with recurrent failed IVF-embryo transfer despite transfer of good quality embryos, compared with other diagnostic methods. We found that SHG is highly valuable and should be applied routinely as a first-line, office-based diagnostic tool in such cases.
Iglesias Miriam - - 2008
OBJECTIVE: To achieve a pregnancy free of X-linked adrenoleukodystrophy (X-ALD) by intracytoplasmic sperm injection (ICSI) and preimplantation genetic diagnosis (PGD). DESIGN: Case report. SETTING: Clínica FIV Recoletos, a private IVF center. PATIENT(S): A couple in which the man had X-ALD. INTERVENTION(S): The ICSI protocol and PGD of the obtained embryos. ...
Ge Hong-Shan - - 2008
The aim of this study was to determine if assisted hatching (AH) could improve the rates of pregnancy and implantation for both fresh and frozen-thawed embryo transfer cycles. A total of 760 fresh embryo transfer cycles and 200 frozen-thawed embryo transfer cycles were randomly assigned to either the treatment group ...
Hiraoka Kenichiro - - 2008
PURPOSE: To evaluate the effect of the size of zona pellucida opening by laser assisted hatching for frozen cleaved embryo that were thawed after both fresh and frozen cleaved embryo transfer cycles failed and were cultured to blastocyst after thawing in patients with multiple implantation failures. MATERIALS AND METHODS: Of ...
Hoozemans D A - - 2008
OBJECTIVE: To evaluate the predictive value of serial uterine artery Doppler ultrasound for embryo implantation in in-vitro fertilization (IVF)-intracytoplasmic sperm injection (ICSI) cycles. METHODS: This was a prospective observational study at the VU University Medical Center, Amsterdam. Patients with an indication for IVF or IVF-ICSI according to departmental protocol underwent ...
Liang X W - - 2008
The objective was to explore the use of sexed sperm and OPU-derived oocytes in an IVP system to produce sex-preselected bubaline embryos. Oocytes were recovered from 20 fertile Murrah and Nili-Ravi buffalo cows by repeated (twice weekly) ultrasound-guided transvaginal ovum pick up (OPU), or by aspiration of abbatoir-derived bubaline ovaries, ...
Gremlich S - - 2008
BACKGROUND: Only 25% of IVF transfer cycles lead to a clinical pregnancy, calling for continued technical progress but also more in depth analysis of patients' individual characteristics. The interleukin-1 (IL-1) system and matrix metalloproteinases (MMPs) are strongly implicated in embryo implantation. The genes coding for IL-1Ra (gene symbol IL-1RN), IL-1beta, ...
Drakeley Andrew J - - 2008
BACKGROUND: We wanted to test the hypothesis that using abdominal ultrasound at the time of embryo transfer to guide replacement, improved pregnancy rates by at least 5%. METHODS: An RCT in a large assisted conception unit. A pilot study and power calculation suggested that at least 2000 embryo transfers were ...
Shu Yimin - - 2009
OBJECTIVE: To investigate the role of fast blastocoele re-expansion in the selection of viable thawed blastocysts for transfer. DESIGN: Retrospective study. SETTING: Academic assisted reproductive program. PATIENT(S): Transfer cycles were divided into two groups according to the presence or absence of fast re-expanded blastocysts. In group I (124 cycles), all ...
Kumasako Yoko - - 2009
OBJECTIVE: To confirm the clinical benefits of recryopreserved, twice-thawed embryo transfer (ET). DESIGN: Retrospective study. SETTING: Private fertility clinic. PATIENT(S): Forty-nine women whose embryos had been refrozen after a previous frozen-thawed ET. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Comparison of implantation and pregnancy rates of twice-cryopreserved, twice-thawed embryos versus once-cryopreserved, once-thawed ...
Alvarez Cristina - - 2008
OBJECTIVE: To determine whether pronuclear morphology is a useful additional criterion when selecting embryos for transfer. METHOD: A modified Tesarik and Greco scoring system was used in this retrospective study to assess the pronuclear morphology of 883 zygotes from 214 in vitro fertilization or intracytoplasmic sperm injection cycles. Pronucleus size ...
Serafini Paulo - - 2008
OBJECTIVE: To determine whether there is an association between endometrial expression of leukemia inhibitory factor (LIF) in the luteal phase of the menstrual cycle preceding in vitro fertilization (IVF) and treatment outcome. METHODS: Biopsy specimens from the endometria of 52 women in the luteal phase were immunostained against LIF. Embryo ...
Loi K - - 2008
INTRODUCTION: The ability to select the embryos that would lead to pregnancy would help to reduce multiple pregnancy rates. The objective was to evaluate the use of a cumulative embryo scoring system (CES) based on a five-point embryo scoring system for the prediction of pregnancy outcome following intracytoplasmic sperm injection ...
Sinclair Kevin D - - 2008
Modern techniques in assisted reproduction have become a common and accepted form of clinical care benefiting an estimated 1 in 10 people of reproductive age who are subfertile or infertile. The success of these technologies relies, to large extent, on the remarkable tolerance of mammalian gametes and the preimplantation embryo ...
Shin Sang Tae - - 2008
This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone ...
Turillazzi Emanuela - - 2008
In 2004, the Italian parliament enacted Law 40/2004, which established new rules for assisted reproductive technology and included a very long and detailed list of restrictions. Since its enactment, the law has given rise to heated discussion at a national and international scientific level. In June 2007 a report presented ...
Esfandiari Navid - - 2008
OBJECTIVE: To report a case of successful singleton pregnancy following a novel technique of sequential double transfer of two groups of frozen embryos at the same developmental stage (8-cell) at 5 and 7 days after the LH surge to a patient with seven previous failed assisted reproductive technology (ART) cycles. ...
Andersen A Nyboe - - 2008
BACKGROUND: European results of assisted reproductive techniques from treatments initiated during 2004 are presented in this eighth report. METHODS: Data were mainly collected from existing national registers. From 29 countries, 785 clinics reported 367,066 treatment cycles including: IVF (114,672), ICSI (167,192), frozen embryo replacement (FER, 71,997), egg donation (ED, 10 ...
Manheimer Eric - - 2008
To evaluate whether acupuncture improves rates of pregnancy and live birth when used as an adjuvant treatment to embryo transfer in women undergoing in vitro fertilisation. Systematic review and meta-analysis. Medline, Cochrane Central, Embase, Chinese Biomedical Database, hand searched abstracts, and reference lists. Review methods Eligible studies were randomised controlled ...
Dayal Molina B - - 2009
OBJECTIVE: To evaluate the impact on the rates of clinical pregnancy and live birth of polyploidy after intracytoplasmic sperm injection (ICSI). DESIGN: Retrospective cohort study. SETTING: University-based IVF center. PATIENT(S): One hundred forty-three patients undergoing their first IVF-embryo transfer cycle requiring ICSI. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Patients were divided ...
La Sala Giovanni Battista - - 2009
OBJECTIVE: To evaluate the effect of selecting three best oocytes for insemination and transfer of all resultant embryos on outcomes of assisted reproductive technologies (ART). STUDY DESIGN: Comparative study. SETTING: The Center of Reproductive Medicine, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. PATIENT(S): Women undergoing ART during the period March ...
Pagidas K - - 2008
OBJECTIVE: To determine the predictive value of euploid embryos in women with recurrent implantation failure undergoing repeated IVF-ET cycles with PGD (PGD). DESIGN: Cohort of IVF-PGD cycles in a tertiary care ART facility. MATERIALS AND METHOD(S): Fifty-five consecutive patients with repeated implantation failure (more than three failed IVF-ET cycles) underwent ...
Youssry M - - 2008
Cryopreservation of human gametes and embryos has become an essential part of assisted reproduction. Successful cryopreservation of human blastocysts is increasingly relevant as extended in-vitro culture of human embryos becomes more common, permitting routine use of blastocyst transfer in IVF programmes. This reduces the number of embryos transferred, thereby reducing ...
Moutou Céline - - 2008
BACKGROUND: Spinocerebellar ataxia 2 (SCA2) is an autosomal-dominant neurodegenerative disease caused by an extended polyglutamine sequence in the ATXN2 protein. We describe the development of a new single-cell multiplex PCR protocol for pre-implantation genetic diagnosis (PGD) of SCA2 and its successful clinical application. METHODS: Three duplex tests have been developed, ...
Khalaf Y - - 2008
To examine the clinical pregnancy rate (CPR) and multiple pregnancy rate (MPR) in a large in vitro fertilisation (IVF) programme before and after the introduction of single blastocyst transfer (SBT) strategy in a selected group of women. A 3-year pre- and postintervention study. A tertiary reproductive medicine and assisted conception ...
Isachenko Vladimir - - 2008
BACKGROUND: The aim of this study was to examine whether the integrity rate of pronuclei, after cryopreservation of pronuclear zygotes, could be a predictor of future embryo development and implantation. METHODS: Two-pronuclei stage zygotes (n = 862) were cryopreserved by aseptic rapid freezing in 15% ethylene glycol + 15% DMSO ...
Serna José - - 2008
OBJECTIVE: To evaluate the effectiveness of transdermal E(2) administration in the luteal phase of IVF/ICSI cycles. DESIGN: Prospective, open-label, randomized clinical trial. SETTING: University-affiliated assisted reproduction center. PATIENTS: 1) Pilot trial to test serum E(2) behaviour during the luteal phase in women undergoing agonist as well as antagonist protocol; 2) ...
Levens Eric D - - 2008
To assess cycle outcome among day 5 and day 6 cryopreserved frozen-thawed blastocyst embryo transfers (FBET). Retrospective cohort study. Military-based assisted reproduction technology (ART) center. One hundred seventy-two nondonor, programmed cryopreserved embryo cycles. Fully expanded blastocysts on day 5 were cryopreserved on day 5, and those achieving this state on ...
Poikkeus Piia - - 2008
Singleton pregnancies following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are associated with increased risks of abnormal placentation, pre-eclampsia and preterm birth. These risks might partly be a consequence of the number of transferred embryos. In this commentary we summarize the results of three observational studies and one ...
Delbaere I - - 2008
The aim of this thesis is to define determinants of impaired implantation and adverse pregnancy outcome in a retrospective manner. Our results demonstrate that pregnancy outcome in singletons of older mothers (> or = 35 years) is less favourable when compared with younger mothers (25-29 years). Remarkably, the same can ...
Van Peperstraten A M - - 2008
BACKGROUND: Some 84% of all European in vitro fertilisation (IVF) and intracytoplasmatic sperm injection (ICSI) cycles is performed with the transfer of more than 1 embryo, with 22% resulting in twin pregnancies. At many centres, the choice for one or more embryos is made through a shared decision-making process. To ...
Yilmaz S - - 2008
Excess embryos obtained from intracytoplasmic sperm injection cycles may be cultured and observed until day 5 if the couple receiving treatment do not want them to be cryopreserved. In order to investigate the correlation between blastocyst formation in extended culture and pregnancy outcome, 194 patients treated in two separate IVF ...
Kuliev Anver - - 2008
Despite recent controversy, existing experience suggests that aneuploidy testing has had a significant impact on the reproductive outcome of poor prognosis IVF patients, which is based on the experience of over 20,000 cases of PGD for chromosomal disorders. The clinical impact was demonstrated in the improved implantation and pregnancy rates, ...
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