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Smrkolj Tomaz - - 2010
In the management of obstructive azoospermia (OA), microsurgery is often replaced by testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). Testicular biopsy and microsurgical side-to-side epididymovasostomy were performed in 34 azoospermic men with OA mostly due to inflammation. Ductal system patency was recovered in 21 (63.6%) men and natural ...
van Roijen J Herman - - 2010
We describe two patients who underwent bilateral epididymovasostomy and subsequent percutaneous epididymal sperm aspiration (PESA) because of persistent azoospermia, after which sperm returned to the ejaculates of both patients, resulting in an unexpected natural pregnancy in one of the couples. Two men, Patient A (age 35 years) and Patient B ...
Hindryckx An - - 2010
BACKGROUND: Although intracytoplasmic sperm injection (ICSI) is now accepted as the treatment of choice for severe male infertility, concerns about its safety and the potential risks for the offspring remain. We reviewed the literature with respect to the prevalence of major congenital malformations after the ICSI technique and supplemented these ...
Citrino G - - 2010
To carry out a retrospectively performed matched controlled study to determine the efficacy of pretreatment of sperm having low hypo-osmotic swelling (HOS) test scores with chymotrypsin galactose prior to intrauterine insemination (IUI) compared to IUI for other types of male factor problems. The women with male partners with low HOS ...
Smit Marij - - 2010
PURPOSE: We prospectively evaluated changes in sperm chromatin structure in infertile patients before and after surgical repair of varicocele, and the impact on the pregnancy rate. MATERIALS AND METHODS: Included in the study were 49 men with at least a 1-year history of infertility, a palpable varicocele and oligospermia. World ...
Check J H - - 2010
To present strategies in diagnosing and treating infertility related to antisperm antibodies. Antisperm antibodies (ASA) were detected on sperm using the direct immunobead (IBD) test. Treatments included intrauterine insemination (IUI) with pretreatment with chymotrypsin/galactose vs. in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). Intrauterine insemination with protein digestive enzyme ...
Bhakta Rajesh - - 2010
To evaluate the effectiveness of two different systems i.e. circulating water bath and carbon dioxide (CO(2)) incubator in extracting motile sperm for IUI programme and their effect on pregnancy outcome. The study was performed on sixty-two patients recruited for ovulation induction followed by intrauterine insemination (IUI) in University fertility clinic. ...
Taylor S L SL The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, - - 2010
Globozoospermia is an infrequent pathology in which spermatozoa lack acrosomes. Patients are considered sterile without IVF augmented with intracytoplasmic sperm injection (ICSI), as fertilization is impaired due to absence of oocyte activation. As far as is known, this is the first study to report results of a comprehensive approach to ...
Smit Marij - - 2010
PURPOSE: We evaluated sperm DNA fragmentation in patients with vasectomy reversal and its prognostic value to determine spontaneous and assisted reproductive technique pregnancy rates. MATERIALS AND METHODS: We prospectively assessed DNA fragmentation with the sperm chromatin structure assay in postoperative semen samples of 70 patients with vasectomy reversal. At a ...
Mendeluk G R - - 2009
We propose that evaluation of protein tyrosine phosphorylation (TP) status in ejaculated spermatozoa under capacitating conditions in an experiment that mimics "in vitro" the physiology of sperm from ejaculation through the female genital tract could potentially be used as a prognostic test for functional competence of sperm in fertilization. Our ...
Magheli A - - 2010
The objective of this study was to determine the effect of the obstructive interval and the presence of a sperm granuloma on vas patency and pregnancy rate following vasectomy reversal. We identified 334 patients with complete follow-up who met the inclusion criteria. There were significant associations between the obstructive interval ...
Kumbak Banu - - 2009
OBJECTIVE: To evaluate the outcome in couples composed of azoospermia and a poor responder female undergoing assisted reproductive techniques (ARTs). STUDY DESIGN: A retrospective study was performed involving 97 men suffering from nonobstructive azoospermia (NOA) whose partners had a poor response to ovarian stimulation. Poor response was defined as retrieval ...
Javed Murid - - 2010
Intracytoplasmic sperm injection (ICSI) has resulted in pregnancy and birth for many couples, including those with severe male factor infertility. However, even after ICSI, complete failure of fertilization occurs in 1-3% of cycles. Most cases occur due to low number of mature oocytes, failure of oocyte activation or non-availability of ...
Black Marlene - - 2010
This study was to compare the outcomes of conventional (scientist-selected sperm) intracytoplasmic sperm injection (ICSI) and a modified ICSI using zona pellucida (ZP)-bound sperm. Although with 39 couples in each group there was no statistical significance in fertilization, embryo development, implantation, and fetal heart clinical pregnancy rates, the higher implantation ...
Chun Sang Sik - - 2010
In this prospective clinical study involving 354 IVF-intracytoplasmic sperm injection cycles, we determined the influence of the length of the uterine cavity on clinical pregnancy rates. Our data showed that clinical pregnancy and implantation rates are associated positively with an increased length of the uterine cavity.
García-Herrero Sandra - - 2010
OBJECTIVE: To differentiate transcripts' expression in the sperm from patients who achieved pregnancy in their first IUI cycle from those who did not. Basic sperm analysis is limited to forecasting pregnancies by means of assisted reproduction. New assays, such as microarray analysis, are potential predictive tools for this purpose. DESIGN: ...
Mart?nez M Carmen - - 2010
In our series, a very high prevalence of chromosomal abnormalities was observed in spontaneous miscarriages, irrespective of how pregnancy was achieved, by assisted reproductive technology (ART) or natural conception. This high prevalence was equally represented in both groups, with no statistical difference in type of chromosomal abnormalities or in total ...
Fauque Patricia - - 2009
The objective of this retrospective study was to describe a population of patients displaying impaired sperm motility due to ultrastructural flagellar defects and to analyse the intracytoplasmic sperm injection (ICSI) results and neonatal outcomes in this population. The fertilization rate, embryo quality, clinical pregnancy rate, implantation rate, birth rate and ...
Underwood S L - - 2010
Two field trials were conducted to determine the fertilising capacity of (i) frozen-thawed, sex-sorted re-frozen-thawed (FSF) dairy bull sperm inseminated close to the time of ovulation, (ii) FSF sperm following large dose insemination, and frozen-thawed, sex-sorted (FS) sperm inseminated within 12h after sorting. In Trial 1, 24 heifers in synchronised ...
Borini Andrea - - 2009
The aim of this work was to evaluate the efficiency of IVF and intracytoplasmic sperm injection (ICSI) when few eggs available for insemination. A total of 601 women (group A, mean age 31.2 +/- 2.8 years) who were undergoing a total of 671 assisted reproduction cycles donated their excess oocytes ...
Haimov-Kochman R - - 2010
The aetiology of cryptorchidism is still undiscernible in the majority of cases. It has long been argued that cryptorchidism reflects a primary testicular maldevelopment, where the contralateral scrotal testis also suffers from aspermatogenesis and low spermatogonia count. The aim of the study was to determine the reproductive outcome of ex-cryptorchid ...
Banker Manish R - - 2009
Globozoospermia is a severe form of teratozoospermia characterized by round-headed acrosomeless spermatozoa. Here we present two successful pregnancies and a live birth after intracytoplasmic sperm injection.
Al-Fifi Suliman - - 2009
Intracytoplasmic sperm injection (ICSI) procedures have become accepted worldwide and their effect on society is well-known. However, the full extent of the possible complications of these procedures on maternal and neonatal outcome is still unclear. This is a retrospective case controlled study from January 2003 to December 2007 which compared ...
Ozguner Meltem - - 2009
A prospective study was planned to determine the relationship between post swim-up acrosome index (AI) evaluation and fertilization outcomes in an in vitro fertilization (IVF) program. Infertile couples who have applied to IVF were admitted into this study when the male partner's sperm concentration was > 20 x 10(6)/mL and ...
Gil-Villa Aura María - - 2010
OBJECTIVE: To evaluate and compare standard sperm parameters, lipid peroxidation of sperm plasma membranes, antioxidant capacity of seminal plasma, and sperm chromatin integrity in ejaculates from men whose partners have a history of recurrent pregnancy loss and from a control group of men with recent fertility. DESIGN: Descriptive study. SETTING: ...
Ferreira Renata Cristina - - 2010
OBJECTIVE: To evaluate the effect of male age on clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles, according to sperm concentration. DESIGN: Retrospective, observational study. SETTING: Assisted reproduction center. PATIENT(S): The study included 1,024 couples undergoing ICSI cycles with fresh spermatozoa. INTERVENTION(S): The influence of paternal age on ICSI outcomes ...
Beydoun Hind A - - 2009
OBJECTIVES: To examine the effect of polycystic ovary syndrome (PCOS), a marker of the metabolic syndrome, on selected indicators of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment success. METHODS: A retrospective cohort study was conducted using existing data on 69 IVF/ICSI treatment cycles undergone by PCOS women ...
Kashima Katsunori - - 2009
In this study, the efficacy and safety of assisted reproduction techniques with the sperm-washing method and nested PCR assay were evaluated in HIV-1-discordant couples, as many HIV-1-positive people of reproductive age are getting married and wish to have children safely. Twenty-seven HIV-1-discordant couples (husband, positive; wife, negative) were enrolled in ...
Sripada Sreebala - - 2010
OBJECTIVE: To explore the association between semen parameters and spontaneous pregnancy. DESIGN: Case-control study. SETTING: Aberdeen Fertility Centre, Aberdeen, Scotland. PATIENT(S): A total of 1,426 untreated couples attending a subfertility clinic with no evidence of azoospermia, anovulation, or tubal disease were observed for 180 weeks. "Cases" were couples who achieved ...
Avendaño Conrado - - 2010
OBJECTIVE: To evaluate DNA fragmentation in morphologically normal sperm recovered from the same sample used for intracytoplasmic sperm injection (ICSI) and to correlate DNA damage with embryo quality and pregnancy outcome. DESIGN: Prospective study. SETTING: Academic center. PATIENT(S): 36 infertile men participating in the ICSI program. INTERVENTION(S): Terminal deoxynucleotidyl transferase-mediated ...
Akarsu C - - 2009
Hypogonadotrophic hypogonadism is characterized by oligospermia or azoospermia and low testosterone, FSH and LH concentrations. In such cases, treatment with gonadotrophins is required to produce or increase spermatozoa in the ejaculate but few achieve normal spermatogenesis. After long periods of medical treatment, if the patients still have a low sperm ...
Ghanem Hussein - - 2010
OBJECTIVE: To assess the effect of treatment with a combination of clomiphene citrate as an antiestrogen and vitamin E as an antioxidant on the incidence of pregnancy and sperm variables in men with idiopathic oligozoospermia and infertility. DESIGN: Prospective, randomized, placebo-controlled trial. SETTING: The outpatient andrology clinic at a university ...
MiciƄski Piotr - - 2009
In this study 60 couples undergoing intracytoplasmic sperm injection (ICSI) procedures were enrolled. All men were classified into two groups regarding to the DNA fractionation index (DFI) threshold value: group I < 15% and group II =or> 15%. In group I, median DFI was 4%, normal pre-implantation embryo development was ...
Tachibana Masahito - - 2009
Although intracytoplasmic sperm injection (ICSI) is a widely used assisted reproductive technique, the fertilization rates and pregnancy rates of immature spermatids especially in round spermatid injection (ROSI) remain very low. During mammalian fertilization, the sperm typically introduces its own centrosome which then acts as a microtubule organizing center (MTOC) and ...
Gambera Laura - - 2010
To perform intracytoplasmic sperm injection (ICSI) in couples with primary infertility owing to sperm defects causing total immotility. Case report. Couple Sterility Center, University of Siena. Two infertile couples, the male members of which had "detached tail" genetic sperm defect. Physical and hormonal assays, semen analysis by light and electron ...
Kushnir Vitaly A - - 2009
To compare aneuploidy rates in first trimester pregnancy losses following IVF+/-ICSI. A retrospective cohort analysis of karyotypes of abortuses following conventional IVF (n=159) and ICSI (n=196). 50.1% of losses were found to be cytogenetically abnormal among all patients undergoing IVF+/-ICSI. A significant increase in fetal aneuploidy rate was noted with ...
Kyrou Dimitra - - 2010
OBJECTIVE: To evaluate the incidence of preeclampsia after intrauterine insemination (IUI) with either donor's or partner's sperm in women with primary infertility. DESIGN: Retrospective cohort study. SETTING: Tertiary referral center. PATIENTS: Between January 1999 and December 2006, 823 women who achieved pregnancy after IUI and delivered at > or =24 ...
French Dan B - - 2010
To determine if strict morphology correlates with outcome parameters in couples undergoing intracytoplasmic sperm injection (ICSI). Retrospective review. Academic nonprofit IVF center. Couples undergoing IVF/ICSI. In vitro fertilization and ICSI. Samples were evaluated for total sperm count, motlity, progression, and morphology using Kruger's strict criteria. The ICSI cycle outcome parameters ...
Zorn Branko - - 2009
Factors that influenced the clinical results of 220 first-attempt intracytoplasmic sperm injection (ICSI) cycles with testicular spermatozoa were evaluated in 107 men with non-obstructive azoospermia, 72 with obstructive azoospermia and 41 with aspermia. Linear and logistic regression analysis showed that the fertilization rate depended positively on Johnsen score (P = ...
Fitzpatrick John L - - 2009
Sperm competition, the contest among ejaculates from rival males to fertilize ova of a female, is a common and powerful evolutionary force influencing ejaculate traits. During competitive interactions between ejaculates, longer and faster spermatozoa are expected to have an edge; however, to date, there has been mixed support for this ...
Manno Massimo - - 2009
OBJECTIVE: To describe a bigeminal pregnancy obtained with a homologous intracytoplasmic sperm injection cycle in a patient with high-grade mosaic Turner syndrome (45,XO/47,XXX 97.5%/2.5%) with bicuspid aortic valve. DESIGN: Case report. SETTING: Unit of Pathophysiology of Human Reproduction in a general hospital. PATIENT(S): Patient with mosaic Turner syndrome with bicuspid ...
Brito L F C - - 2010
A 5-year-old Arabian stallion was managed for breeding with fresh/extended semen during a period of 8 months with a resulting per cycle pregnancy rate of 26.3%. The stallion was in good health and no abnormalities of the reproductive tract were observed. Evaluation of several ejaculates revealed that sperm production and ...
Gojnic M - - 2009
The case of a 29-year-old patient who underwent invitro fertilization due to secondary sterility, after a spontaneous but molar pregnancy, is described. Afterwards there was no spontaneous conception for five years. With the couple's consent, we decided to perform in vitro fertilization, i.e., ovulation induction and the ICSI (intracytoplasmic sperm ...
Check J H - - 2009
PURPOSE: To determine in cases of unexplained infertility whether conventional oocyte insemination vs. intracytoplasmic sperm injection results in differences in fertilization rates, frequency of failed fertilization, clinical and live delivered pregnancy rates, and implantation rates. METHODS: Retrospective evaluation of these parameters in couples undergoing in vitro fertilization embryo transfer (IVF-ET) ...
Busato Wilson F S WF - - 2009
INTRODUCTION: Since the demand for vasectomy reversal is increasing and many populations in Brazil and other countries show distinct characteristics, this study was carried out as an effort to determine factors and characteristics associated with the success rate of reversal in a population in Southern Brazil. PATIENTS AND METHODS: We ...
Lee Yuk Tong - - 2009
OBJECTIVES:Colonoscopy cannot be completed in up to 10% of cases. We postulate that cap-assisted colonoscopy (CAC), by fitting a mucosectomy cap to the tip of a colonoscope, could improve the outcome. METHODS:We conducted a prospective randomized controlled trial in two regional endoscopy centers. All colonoscopies were performed by experienced colonoscopists. ...
Rigot J M - - 2009
In the non-obstructive azoospermia versus the secretory azoospermia it is first necessary to have a clear assessment and definition and then takes care of the patient before the surgical sampling taking into account the age of the woman and in fine try to optimize the residual spermatogenesis. Biopsy seems to ...
Simmons L W - - 2009
Recent in vitro fertilization studies have revealed female and male x female interaction effects on the probability of fertilization. These findings suggest a mechanism of cryptic female choice via sperm-egg interactions. The egg jelly of anuran amphibians contains proteins that facilitate the chemoattraction and binding of sperm for fertilization. Here ...
Colleoni S - - 2009
Sorting of sperm by flow cytometer has allowed selection of offspring of predetermined sex in several species by artificial insemination, although the success rate is often lower than with non-sexed semen. In horses, the problem was partially overcome with hysteroscopic insemination using sex-sorted fresh sperm. However, when sex-sorted frozen-thawed sperm ...
Desai Nina - - 2009
This study takes an in depth look at embryonic development, implantation, pregnancy and live birth rates with frozen epididymal and testicular sperm from obstructed (OA) and non-obstructed (NOA) patients. Paternal effect of sperm source on zygote formation, embryonic cleavage, and genomic activation were examined. Additional outcome parameters monitored were clinical ...
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