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Rahat Beenish B Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, - - 2014
Placental development is known for its resemblance with tumor development, such as in the expression of oncogenes (c-myc) and telomerase (hTERT). The expression of c-myc and hTERT is upregulated during early pregnancy and gestational trophoblastic diseases (GTDs). To determine the role of DNA methylation [via methylation specific-high resolution melting (MS-HRM)]and ...
Ura Blendi B Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy. Electronic address: - - 2014
To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24). Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with ...
Sundrani Deepali P DP 1Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, Maharashtra, - - 2014
Introduction:Altered angiogenesis has been implicated in the pathogenesis of various pregnancy complications, particularly preeclampsia. At present, there is a lack of data on the possible role of angiogenesis and its molecular mechanism in preterm pregnancy. We have previously reported reduced placental global DNA methylation levels in preterm pregnancy. Now, we ...
Whitehead Clare L CL Translational Obstetrics Group, Department of Obstetrics and Gynaecology, University of Melbourne, Mercy Hospital for Women, 163 Studley Rd., Heidelberg 3084, Victoria, - - 2014
Severe fetal hypoxia causes stillbirth and permanent childhood disability. Unfortunately, none of the current tests is precise at determining the degree of fetal hypoxia in utero. We recently showed that hypoxia-induced RNA abundance in the maternal circulation (of likely fetoplacental origin) was tightly correlated with the degree of fetal hypoxia, ...
Tsui Nancy B Y NB Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health - - 2014
Analysis of circulating RNA in the plasma of pregnant women has the potential to serve as a powerful tool for noninvasive prenatal testing and research. However, detection of circulating RNA in the plasma in an unbiased and high-throughput manner has been technically challenging. Therefore, only a limited number of circulating ...
Huang Jin J Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, - - 2014
To examine the possible association between high fetal nuchal translucency (NT) thickness and pathogenic chromosomal copy number variants (CNVs) by array comparative genomic hybridization (CGH) in pregnancies with normal fetal karyotype. Array CGH was carried out in stored samples of chorionic villi from 215 singleton pregnancies resulting in live births ...
Seo Heewon - - 2014
Prostaglandins (PGs) are involved in many reproductive activities including luteolysis, maternal recognition of pregnancy, endometrial gene expression, conceptus development, and parturition in domestic animals. However, mechanisms by which PGE2 and PGF2alpha are modulated in the uterine endometrium and expression of ABCC4 and SLCO2A1, responsible for efficient transport of PGs across ...
Ducza Eszter E Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, H-6720, Eötvös u. 6., - - 2014
Aquaporins (AQPs) are integral membrane channels responsible for the transport of water across a cell membrane. Based on reports that AQPs are present and accumulate in the female reproductive tract late in pregnancy, our aim was to study the expression of AQP isoforms (AQP1, 2, 3, 5, 8, and 9) ...
Blois Sandra M SM Charité Center 12 Internal Medicine and Dermatology, Reproductive Medicine Research Group, Medicine University Berlin, Berlin, - - 2014
Galectin-1 (gal-1) is a prototype carbohydrate-binding protein, whose dysregulation is associated with adverse pregnancy outcomes such as spontaneous abortion and preeclampsia. Furthermore, it is known that faulty gal-1 protein production or gene regulation can be caused by single nucleotide polymorphisms in the LGALS1 gene. Gestational diabetes mellitus (GDM) is also ...
Teerapornpuntakit Jarinthorn J Department of Physiology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, - - 2014
In pregnancy and lactation, maternal adaptation for the enhancement of intestinal ion and nutrient absorption is of paramount importance for fetal development and lactogenesis. This nutrient hyperabsorption has been reported to result from upregulation of transporter gene expression, in part, under control of lactogenic hormone prolactin (PRL). Since a number ...
Charan Poonam P Pauline Gandel Imaging Centre, Royal Women's Hospital, Parkville, Victoria, Australia; Fetal Medicine Unit, Royal Women's Hospital, Parkville, Victoria, - - 2014
The main aim of this study was to determine the feasibility of using high-resolution microarray to assist with prenatal diagnosis of ultrasound-detected fetal abnormality and to describe the frequency of abnormal results in different categories of fetal anomalies. Prospective cross-sectional study was conducted on women diagnosed with a fetal anomaly ...
Xu Xinyun X Department of Preventive Medicine, School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. - - 2014
The present study examined the placental leptin (LEP) DNA methylation and mRNA levels in macrosomic infants from normal pregnancies. In total, 49 neonates with macrosomia, i.e., high birth weights of ≥4,000 g, and 52 neonates with normal birth weights between 2,500 g and 4,000 g were recruited from The Second Affiliated Hospital of Wenzhou Medical University (Wenzhou, ...
Xu J-Y JY Institute of Reproductive Immunology, College of Life Science and Technology, Jinan University , Guangzhou , People's Republic of - - 2014
Genetic mutations in the von Hippel-Lindau (VHL) gene are common in certain diseases. The effects on the VHL gene in the tubal pregnancy tissues are unknown but with further study, it was found that the VHL gene may be related to prognosis or therapy selection. This study was conducted to ...
Lucariello Angela A 0000-0001-6435-7680 Department of Mental and Physical Health and Preventive Medicine, Section of Human Anatomy, Second University of Naples, Largo Madonna delle Grazie, 80138 Naples, - - 2014
The WFS1 gene, encoding a transmembrane glycoprotein of the endoplasmic reticulum called wolframin, is mutated in Wolfram syndrome, an autosomal recessive disorder defined by the association of diabetes mellitus, optic atrophy, and further organ abnormalities. Disruption of the WFS1 gene in mice causes progressive β -cell loss in the pancreas ...
Dominguez Francisco F Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain; INCLIVA Biomedical Research, Valencia, - - 2014
Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA ...
Adler Alexis A New York University Fertility Center, New York University Langone School of Medicine, 660 1st Ave., 5th Floor, New York, NY 10016, United States. Electronic address: - - 2013
Preimplantation genetic diagnosis and screening improves the chances of achieving a viable pregnancy, not only free of undesired single-gene defects but also aneuploidy. In addition, improvements in vitrification provide an efficient means of preserving embryos (blastocysts). By combining trophectoderm biopsy with recent improvements in vitrification methods, only those embryos that ...
Phillipps Hollian R HR Centre for Neuroendocrinology and Department of Anatomy, School of Medical Sciences, University of Otago Dunedin, 9016, New - - 2013
Hyperphagia and weight gain to acquire energy stores for development and growth of the fetus and to prepare for the demands of lactation are important adaptations to support a healthy pregnancy. As a consequence, hypothalamic leptin resistance develops to enable maintenance of a positive energy state. During pregnancy there is ...
Shue Eveline H EH Department of Surgery, Division of Pediatric Surgery and Fetal Treatment Center, University of California, San Francisco, San Francisco, - - 2014
Pulmonary hypertension (pHTN), a main determinant of survival in congenital diaphragmatic hernia (CDH), results from in utero vascular remodeling. Phosphodiesterase type 5 (PDE5) inhibitors have never been used antenatally to treat pHTN. The purpose of this study is to determine if antenatal PDE5 inhibitors can prevent pHTN in the fetal ...
Tandiya Ujjawala - - 2013
The objective of this study was to characterize the temporal profile of pregnancy-associated glycoproteins (PAGs; isoforms 1-11) across different stages of gestation in the Barbari goat. Placentae were collected from local abattoir, classified according to crown rump length of the corresponding foetus into five groups (0-30, 31-60, 61-90, 91-120, and ...
Iciek R - - 2013
Type 1 diabetes mellitus (T1DM) is still associated with increased risk for severe maternal and fetal complications but their pathomechanism remains unclear. We investigated into possible role of placental leptin (LEP) and its receptor gene (LEPR) in T1DM pregnancies. Fourty nine pregnant women with T1DM and singleton pregnancy were enrolled ...
King V - - 2013
Exposure to overnutrition in utero may increase offspring cardiometabolic disease risk. A mouse model of maternal exposure to an obesogenic diet (DIO) was used to determine effects on fetal and placental weight and gene expression in mid- and late gestation. DIO altered placental gene expression in mid-gestation without differences in ...
Novembri R - - 2013
11β-hydroxysteroid dehydrogenase 1 and 2 (11β-HSD1 and 11β-HSD2) are involved in the complex mechanism of human parturition. The present study examined mRNA expression and activity of membrane 11β-HSD1 and placental 11β-HSD2 in postdate pregnancies according to response of labor induction. In comparison to postdate women who had spontaneous delivery or ...
Spencer Thomas E TE Department of Animal Sciences, Center for Reproductive Biology, Washington State University, Pullman, Washington, USA. - - 2013
In cattle, the blastocyst hatches from the zona pellucida on days 8-9 and then forms a conceptus that grows and elongates into an ovoid and then filamentous shape between days 9 and 16. The growing conceptus synthesizes and secretes prostaglandins (PGs) and interferon τ (IFNT). Our hypothesis was that the ...
Koldehoff Michael - - 2013
We examined the molecular characteristics of monocytes of pregnant and non-pregnant women to investigate the molecular effects that are associated with immunoregulation at the maternal-fetal interface. We analyzed molecular features and target genes in monocytes of pregnant women using flow cytometry, real-time PCR and oligonucleotide microarray technology. CD14(high) monocytes and ...
de Wit M C - - 2013
Since genomic microarray became available as a diagnostic instrument in the prenatal setting, knowledge about the chance of revealing causative submicroscopic copy number variants (CNVs) in pregnancies with ultrasound anomalies is essential in prenatal care. The prevalence of pathogenic submicroscopic CNVs in fetuses with a structural ultrasound anomaly restricted to ...
Hentschke Marta Ribeiro - - 2013
Artherosis of spiral arteries in uteroplacental beds from pre-eclamptic women resemble those of atherosclerosis, characterised by increased plasma lipids and lipoproteins. We hypothesised 1) lipoproteins receptors/transporter in placenta would be up-regulated in pre-eclampsia, associated with increased maternal and fetal lipoprotein concentrations; 2) expression of these would be reduced in pre-eclamptic ...
Joergensen M W - - 2013
When a triploid pregnancy is diagnosed prenatally, gynaecologists have traditionally relied on the histopathological examination of the tissue from the terminated pregnancy to determine if the pregnancy is molar. However, reproducibility is poor and variability is high when diagnosing hydatidiform moles. Triploid pregnancies can have either the chromosomal constitution of ...
Kandasamy S - - 2013
Ghrelin, a novel motilin-related endogenous ligand for growth hormone secretagouge receptor, is implicated in various biological functions, including regulation of female reproduction. But the presence of ghrelin and its role in reproductive functions in buffalo, a species with poor reproductive efficiency, is not known. In the present study full-length ghrelin ...
Brown Lucia - - 2013
Recurrent hydatidiform moles is an uncommon occurrence. Over the past decade, genetic studies of women with multiple recurrent molar pregnancies have revealed that maternal mutations in two different genes, NLRP7 and C6orf221, result in recurrent moles. We report a 23 year old woman, born of unrelated parents, who has experienced ...
Grotegut Chad A - - 2013
Obese pregnant women have higher rates of dysfunctional labor patterns, need for oxytocin augmentation, labor induction, postdates pregnancy, and cesarean delivery compared to normal weight pregnant women. We tested the hypothesis that myometrial oxytocin receptor (OXTR) gene and protein expression are affected by obesity in pregnancy. Myometrial samples were obtained ...
Jian Feng - - 2013
PURPOSE: The changes of leukotriene B4 (LTB4) in the serum and the 5-lipoxygenase (5-LO) in the decidua in different stages of gestation period were investigated. METHODS: The ELISA double antibody sandwich method was used to detect the concentration of LTB4 in the serum in 21 cases and its levels of ...
Novelli Antonio - - 2013
Evaluation of: Wapner RJ, Martin CL, Levy B et al. Chromosomal microarray versus karyotyping for prenatal diagnosis. N. Engl. J. Med. 367(23), 2175-2184 (2012). Prenatal diagnosis is now offered to high-risk pregnancies, including advanced maternal age, ultrasound anomalies and positive Down's syndrome screening, and karyotype on cultured fetal material is ...
Weix Janine - - 2013
During pregnancy, most patients with rheumatoid arthritis (RA) experience a spontaneous improvement in their condition. Since type I interferons (IFN) have immunomodulatory properties, we investigated whether type I IFN-inducible genes are upregulated in pregnant patients with RA. Peripheral blood mononuclear cells were evaluated using quantitative real-time polymerase chain reaction for ...
Kanenishi Kenji - - 2013
OBJECTIVE: To assess the frequency of fetal facial expressions at 25-27weeks of gestation using 4D ultrasound. METHODS: Twenty-four normal fetuses were examined using 4D ultrasound. The face of each fetus was recorded continuously for 15minutes. The frequencies of tongue expulsion, yawning, sucking, mouthing, blinking, scowling, and smiling were assessed and ...
Ventura Walter - - 2013
OBJECTIVE: First, to determine if microRNA-17 and -19b are expressed in villous samples at early stages of pregnancy. Second, to determine whether placental expressions of these microRNAs along with their main targets (PTEN, CREB-1, TGFβ-1 and TGFβ-RII) are altered in early pregnancy loss. STUDY DESIGN: Expression levels of microRNAs and ...
Whitehead Clare L - - 2013
Context:Fetal growth restriction (FGR) is a leading cause of perinatal mortality, yet no reliable screening test exists. Placental specific mRNA in the maternal circulation may reflect changes in the placental transcriptome in FGR and could be a novel biomarker for FGR.Objective:The aim of the study was to identify placental specific ...
Yin Guowu - - 2013
The purpose of this study was to investigate the expression of estradiol and estrogen receptor α (ESRα) in severe preeclamptic (sPE) pregnancies compared with normal pregnancies. Sera and placentas were obtained from i) patients with sPE (n=25) and ii) a normal control group (n=25) who underwent elective Cesarean deliveries. Estradiol expression ...
Rull K - - 2013
INTRODUCTION: Recurrent miscarriage (RM; ≥3 consecutive pregnancy losses) occurs in 1-3% of fertile couples. No biomarkers with high predictive value of threatening miscarriage have been identified. We aimed to profile whole-genome differential gene expression in RM placental tissue, and to determine the protein levels of identified loci in maternal sera ...
Baker Tieneka M - - 2013
Bardet-Biedl syndrome (BBS) is an autosomal recessive ciliopathic human genetic disorder with variable expression that is difficult to diagnose in pregnancy without known risk factors. Homozygosity testing has been shown to be a useful tool in identifying BBS mutations and candidate genes in affected individuals. We present the first case ...
Tannetta Dionne S - - 2013
The placental syncytiotrophoblast releases micro and nanovesicles (STBM), into the maternal circulation in normal pregnancy and in increased amounts in pre-eclampsia (PE), which have proinflammatory and antiangiogenic activity and are implicated in PE pathophysiology. Better characterisation of STBM is essential to understand their role in PE. STBM prepared by placental ...
Rodriguez-Prado Yahdira M - - 2013
PLAC1 (Placenta-Specific 1) is a recently described, trophoblast-expressed gene essential for normal placental development. The protein localizes to the microvillus membrane surface of the syncytiotrophoblast in direct proximity to the maternal compartment. Although its role has not been defined, increased circulating levels of human PLAC1 mRNA in maternal blood are ...
Li Hailing - - 2013
Objective. Preeclampsia (PE) is a pregnancy-specific syndrome and one of the leading causes of maternal and fetal morbidity and mortality. The pathophysiological mechanisms of PE remain poorly known. Recently, circulating miRNAs are considered as potential useful noninvasive biomarkers. The aim of this study was to identify differentially expressed plasma miRNAs ...
Haddad Ghassan G Houston Fertility Institute, New Houston Health, 2500 Fondren Rd,, Suite 350, Houston, TX 77063, USA. - - 2013
High proportions of human embryos produced by in vitro fertilization are aneuploidy and mosaic. DNA microarray is one of the most practical screening methods to select euploid embryos for transfer. However, mosaic pregnancy is still possible due to embryonic mosacism. Here we report a successful pregnancy after transfer of a ...
Textoris Julien J Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, CNRS UMR 7278, INSERM U1095, Marseille, - - 2013
Preeclampsia is a placental disease characterized by hypertension and proteinuria in pregnant women, and it is associated with a high maternal and neonatal morbidity. However, circulating biomarkers that are able to predict the prognosis of preeclampsia are lacking. Thirty-eight women were included in the current study. They consisted of 19 ...
Kwok Simon Cm SC ORTD, Albert Einstein Medical Center, 5501 Old York Road, Philadelphia, PA 19141-3098, USA. - - 2013
Proprotein convertases are a family of serine proteinases that are related to bacterial subtilisin and yeast kexin. They are involved in posttranslational processing of the precursors of a vast number of cellular proteins. With the exception of PC1/3, the relative expression levels of the proprotein convertases in the ovary during ...
Hogg Kirsten - - 2012
In pre-eclampsia, placental leptin is up-regulated and leptin is elevated in maternal plasma. To investigate potential epigenetic regulation of the leptin (LEP) gene in normal and complicated pregnancy, DNA methylation was assessed at multiple reported regulatory regions in placentae from control pregnancies (n=111), and those complicated by early onset pre-eclampsia ...
Yang L - - 2012
The objective was to investigate the expression of insulin-like growth factor 2 receptor (IGF2R), leptin receptor (LEPR), and serum- and glucocorticoid-regulated kinase 1 (SGK1) mRNA in human decidua and bovine pregnancies. The IGF2R, LEPR, and SGK1 mRNA were measured by using quantitative real-time PCR in 25 patients with spontaneous abortion, ...
Derussi Aap - - 2012
The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non-pregnant females underwent OVH 12 days after the pre-ovulatory Luteinizant Hormone (LH) ...
Samborski A - - 2012
Establishment of pregnancy in mammals requires prolongation of luteal life span for sustained progesterone (P4) production. P4 stimulates secretory functions of the endometrium required for conceptus growth and development and is essential for endometrial receptivity. The porcine conceptus undergoes rapid differentiation and expansion of its trophoblastic membranes between Day 11 ...
Iager Amy E - - 2012
OBJECTIVE: To identify a gene expression signature in human cumulus cells (CCs) predictive of pregnancy outcome across multiple clinics, taking into account the clinic and patient variations inherent in IVF practice. DESIGN: Retrospective analysis of single human cumulus-oocyte complexes with the use of a combined microarray and quantitative reverse-transcription polymerase ...
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