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Results 451 - 500 of 680
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Bennett A R - - 1997
Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the separation of specific ...
Blais B W - - 1997
A variety of foods, animal feeds and environmental samples were analyzed for the presence of Salmonella using the polymyxin-cloth enzyme immunoassay (p-CEIA) system. Salmonella lipopolysaccharide (LPS) antigens were captured from test samples on polymyxin-coated polyester cloth, followed by immunoenzymatic detection of bound antigens using a monoclonal antibody recognizing an LPS ...
Cudjoe K S - - 1997
A Dynal core method has been established using Dynabeads anti-Salmonella to detect Salmonella from all categories of food samples. The protocol consists of the standard pre-enrichment of samples in buffered peptone water followed by immunomagnetic separation and subsequent selective enrichment of the bead-bacteria complexes in Rappaport-Vassiliadis Soya Peptone broth before ...
Ripabelli G - - 1997
The aim of the study was to compare immunomagnetic separation (IMS) and conventional selective enrichment procedures using selenite cystine broth (SC) and Rappaport-Vassiliadis broth (RV) in 137 naturally contaminated food samples (69 raw pork sausages and 68 chicken meat). The utilization of SC or IMS appeared to be the most ...
Curiale M S - - 1997
The VIDAS SLM method for detection of Salmonella was compared with the Bacteriological Analytical Manual (BAM)/AOAC culture method in a collaborative study. Twenty laboratories participated in the evaluation. Each laboratory tested one or more of 6 test products: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black ...
Barrett P - - 1997
A study was made of all potentially statutorily notifiable bacterial infections diagnosed in faecal samples submitted from symptomatic patients to a single microbiology laboratory during a six-month period. Salmonella spp, Campylobacter spp or Shigella spp were isolated from 167 patients and 51% of these were formally notified (54% of general ...
Henson S - - 1997
The paper describes the use of the Delphi method to estimate the incidence of food-borne Salmonella in the UK and the effectiveness of alternative control measures. A panel of experts of food-borne Salmonella participated in the Delphi survey, which involved five rounds of questioning taking place in the period July ...
Tolker-Nielsen T - - 1997
In order to assess the usefulness of quantitative in situ rRNA hybridization as an indicator of the physiological state of bacteria, we have used this method to measure the cellular contents of 16 S and 23 S rRNA in Salmonella typhimurium subjected to a number of different stress treatments. The ...
Wegener H C - - 1997
In a survey of vancomycin resistant Enterococcus faecium (VREF) in Danish meat products, VREF could be detected in 16% of 160 samples of broilers collected at slaughterhouses and in 15% of 26 samples of pork collected from the retail trade. VREF were isolated by enrichment for 24 h in nutrient ...
Hanai K - - 1997
Six commercial kits were compared with the U.S. Food and Drug Administration (USFDA) method and the Japanese standard method for Salmonella isolation in foods. When only Salmonella serovars were tested, many of the methods performed well; however, when foods were artificially inoculated, only the USFDA method and immunomagnetic separation coupled ...
Lee T G - - 1997
Leghorn spent hen mechanically deboned chicken meat (MDCM) was utilized in the preparation of a value-added chicken sausage. The sausage was manufactured by combining MDCM with hand-deboned skinless chicken thigh meat, fat replacer ingredients (blend of modified food starch and oat flour), and either 1) no ingredient with antioxidant properties, ...
Mulder R W - - 1997
The revolutionary industrialisation of the poultry industry in the last 30 years has made the food poultry meat available for large groups of consumers. Due to its nutritional, sensory and economical characteristics, poultry meat is by far the most popular animal food product world-wide. Epidemiological reports, however, incriminate poultry meat ...
Palmer S - - 1996
The incidence of acute gastroenteritis and self suspected food poisoning in general practice populations was compared with consultation rates in four group practices during a 3-month winter period and a 2-month autumn period. The average monthly consultation rate for acute gastroenteritis and self suspected food poisoning was 0.3% and 0.06% ...
June G A - - 1996
A collaborative study was performed in 18 laboratories to validate use of Rappaport-Vassiliadis (RV) medium in the standard culture method for recovery of Salmonella spp. from raw, highly contaminated foods and poultry feed. RV medium made from its individual ingredients and incubated at 42 degrees C was compared with selenite ...
Schmidt K A - - 1996
Release of Echinostoma trivolvis cercariae from naturally infected Helisoma trivolvis snails maintained under different laboratory conditions was studied. Infected snails were isolated for 1 hr in Stender dishes containing 5 ml of artificial spring water and the number of cercariae released during this time was recorded. Of the conditions tested, ...
Fierens H - - 1996
Five commercially available screening methods, the Oxoid MSRV, Merck SALMOSYST-RAMBACH AGAR combination, Organon Teknika SALMONELLA-TEKTM, Dynal DYNABEADS ANTI-SALMONELLA and Foss Electric EIAFOSS, were compared to the conventional culture procedure for the detection of Salmonella in naturally contaminated feed samples. A total of 217 feed samples from animal as well as ...
McDermid A S - - 1996
Small particle aerosols of plate-grown Salmonella enteritidis and Salm. typhimurium were generated and maintained within a rotating drum at 75% relative humidity and 24 degrees C for 2 h. Plate-grown organisms were found to be more aerosol-stable than broth-grown organisms. Differences were observed between the two species; plate-grown Salm. typhimurium ...
Nychas G J - - 1996
The effect of vacuum and modified atmosphere packaging on the growth/survival of Salmonella enteritidis on fresh poultry and fish (Boops boops) is described. Salmonella enteritidis survived but did not grow significantly in all samples (poultry or fish) at 3 degrees C. At 10 degrees C the numbers of Salm. enteritidis ...
Davies R H - - 1996
1. Studies on the survival of Salmonella enteritidis in poultry units and food were carried out over a two-year period. 2. The organism persisted for at least one year in an empty trial house at the laboratory in which naturally-infected broiler breeder birds had previously been housed. A similar survival ...
Davies R H - - 1996
A study of the dissemination of Salmonella enteritidis in the poultry breeder industry in the UK showed that the choice of sites for sampling the environment of occupied houses and empty houses which had been disinfected after depopulation had a significant influence on the outcome. Increased isolation rates could be ...
Sasipreeyajan J - - 1996
Salmonellae were detected in thirteen broiler flocks, 15 layer flocks and 7 parent breeder flocks in Thailand from October 1991 to August 1992. Salmonellae were isolated from samples of feed, drinking water, cloacal swabs, faeces and litter from all broiler and breeder flocks, and 87% of the layer flocks. From ...
Wilson I G - - 1996
A prospective survey was carried out in Northern Ireland between February and August 1994 to assess the current levels of salmonella in retail chickens, and to determine whether contamination rates differed between chickens sold in supermarkets and by butchers. One hundred and forty chilled and frozen raw chickens were sampled ...
Altekruse S F - - 1996
A national telephone survey was conducted of 1,620 randomly selected U.S. residents who spoke English, were at least 18 years old, and resided in households with kitchen facilities. Respondents were interviewed about their recognition of foodborne pathogens, foods at risk for transmitting infection, knowledge of safe food handling, and food-handling ...
Ruiz J - - 1996
A comparative study was carried out to evaluate the performances of different culture media for the recovery of Salmonella spp. from 1,000 routine samples of human stools. By direct plating we tested Salmonella-Shigella agar (SS), Hektoen enteric agar (HE), bismuth sulfite agar (BS), novobiocin-brilliant green-glycerol-lactose agar (NBGL) and SM-ID medium ...
Madsen M - - 1996
Salmonella was isolated from 6 of 20 (30.0%) samples of fresh meat, and from 28 of 140 (20.0%) samples of frozen meat processed for human consumption from captive Nile crocodiles (Crocodylus niloticus) in Zimbabwe. Salmonella enterica isolates showed a serovar distribution of 41.2% (14/34) subsp. enterica, 11.8% (4/34) subsp. salamae ...
Fule R P - - 1996
Thirty three patients presented with acute diarrhoea and vomiting, 12-24 h after consuming vegetarian food. Twenty three patients developed high grade fever, with two patients developing complications. Salmonella paratyphi A var durazzo was isolated from 12 faecal samples. This serovar of Salmonella as a causative organism of food poisoning is ...
Hoszowski A - - 1996
A strategy was developed for 24-h detection and enumeration of Salmonella spp. on processed chicken carcasses. Carcasses were rinsed with saline and the rinses spiked with known numbers of serogroup B, C, D or E Salmonella. The total rinse volume was passed through two filter units of decreasing pore size. ...
Chaicumpa W - - 1995
Monoclonal antibody (MAb) produced to polysaccharides in the LPS molecule of salmonellae was used in a dot-blot ELISA for detecting Salmonella in 873 food samples, ie 100 fresh chicken, 261 frozen chicken, 78 pork, 84 beef, 100 hen eggs, 100 duck eggs, 50 sea-mussels, 50 shrimps and 50 squids in ...
Tran T T - - 1995
Recovery limits were evaluated for Campylobacter jejuni in an existing Food and Drug Administration (FDA) enrichment broth (EB) formula supplemented with Oxyrase enzyme. Cultures of Camp. jejuni were inoculated into EB or EB containing 10% raw milk, raw oysters, crabmeat or mushrooms. After 24 and 48 h of enrichment, Camp. ...
Jitrapakdee S - - 1995
A polymerase chain reaction (PCR) method was developed for detection of salmonella in food. A set of PCR primers was designed to amplify a 199 bp salmonella-specific DNA fragment derived from a repetitive DNA of Salmonella Weltevreden. The assay detected all 52 most prevalent serovars found in contaminated food in ...
Corry J E - - 1995
Raw meat, particularly poultry meat, remains an important, and probably the major source of human infection with campylobacters and salmonellas. In spite of decades of effort it has so far proved extremely difficult to raise food animals free of these pathogens. For the foreseeable future, therefore, the most effective approach ...
Nakamura M - - 1995
The tissue distribution of Salmonella enteritidis in intratracheally inoculated chickens and the effect of deprivation of food and water on tissue distributions of the bacteria have been investigated. Seven-week-old specific-pathogen-free chickens were inoculated intratracheally with 10(2), 10(5), or 10(8) cells and orally with 10(5) cells. The intratracheally inoculated organisms entered ...
Evans M R - - 1995
Following a buffet meal served to six guests at a private domestic function, five of the guests and the host developed symptoms of food poisoning. Salmonella enteritidis phage type 4 (PT4) was isolated from all four individuals who submitted faecal samples for investigation. Leftover samples of a savoury rice dish ...
Coleman D J - - 1995
Immunomagnetic separation with additional enrichment was used in conjunction with improved selective media to improve the isolation of salmonellae from raw sausages. The isolation rate achieved was almost double that of conventional culture with no increase in processing time. The selective media gave an overall specificity of approximately 74%; all ...
Curtis L M - - 1995
Campylobacter jejuni was inoculated into a range of raw and cooked foods and survival determined during storage at 2 degrees, 10 degrees and 20 degrees C for up to 56 d. To facilitate easy enumeration, two antibiotic-resistant strains of Camp. jejuni, which had similar survival characteristics to the parent strain, ...
D'Aoust J Y - - 1995
Refrigerated preenrichment 72 h and selective enrichment cultures arising from 25 g analytical units of dry foods can be used to increase the analytical flexibility and productivity of laboratories for the detection of foodborne Salmonella spp. by AOAC method 994.04. Results of this intralaboratory study using artificially contaminated dry foods ...
Feldsine P T - - 1995
A wide variety of naturally contaminated and inoculated raw flesh and highly contaminated food types was analyzed by a modified immunodiffusion enrichment protocol and the Bacteriological Analytical Manual (BAM) method to determine the equivalence of these methods. This modification was developed by Agriculture Canada to allow addition of a high-temperature ...
Brigmon R L - - 1995
An ELISA previously developed for the rapid detection of Salmonella enteritidis (SE) in environmental samples was modified and applied to food samples. A sandwich ELISA was designed that employs affinity-purified rabbit polyclonal antibodies for the capture stage and highly specific monoclonal antibodies for the detection stage. Thirty-nine species of bacteria ...
Feldsine P T - - 1995
The single enrichment immunodiffusion (1-step), the preenrichment and selective enrichment immunodiffusion (2-step), and the AOAC/Bacteriological Analytical Manual culture methods for Salmonella were evaluated for equivalence in 2 separate studies, a comparative evaluation and a multilaboratory dilution study. In the comparative study, all 3 methods were performed on 10 food types. ...
Corry J E - - 1995
The history of the development of selective media for isolation of campylobacters, including the rationale for choice of selective agents is described. Developments have included modifications to allow incubation at 37 degrees C instead of 42 or 43 degrees C and changes in the types and concentrations of antibiotics in ...
Busse M - - 1995
The main categories of selective plating media are reviewed. The selective enrichment media are considered to be of critical importance in salmonella isolation. During the last 10 years Rappaport-Vassiliadis broth has emerged as a new reliable enrichment medium. Likewise motility enrichment is a very promising technique, but will no doubt ...
Stern N J - - 1995
Colonization of the ceca and contamination on carcasses of chickens by Campylobacter spp. was investigated. Samples were taken on the farm and after transport and holding. In the first set of experiments, 20 chickens, obtained from each of 10 broiler farms, were collected from houses containing 6- to 7-wk-old birds. ...
Sherrod P S - - 1995
The relative effectiveness of 6 selective plating media were compared for effectiveness in recovery of Salmonella spp. from selected high-moisture foods. Three new plating agars (EF-18, Rambach, and xylose lysine Tergitol-4) and 3 selective plating agars (bismuth sulfite, Hektoen enteric, and xylose lysine desoxycholate) recommended by AOAC INTERNATIONAL and the ...
Singh B R - - 1995
Cockroaches are inhabitant of sewers and frequent visitors of kitchen and stores in the night to feed on left over. It is liable to disseminate a number of pathogens by contaminating kitchen surface, feeding vessels and food items left open. Salmonella paratyphi B var Java a common pathogen of man ...
Uyttendaele M - - 1995
An assay to detect Campylobacter jejuni in foods that uses a short selective enrichment culture, a simple and rapid isolation procedure, NASBA amplification of RNA, and a nonradioactive in solution hybridization was studied. The presence of high numbers of indigenous flora affected the sensitivity of the assay. However, detection of ...
Reiber M A - - 1995
Two experiments were conducted to determine the effect of inseminating hens with semen contaminated with marker strains of Salmonella enteritidis and S. typhimurium. Eggs were collected and examined for salmonellae. In Expt. 1, 3/57 (5.3%) of eggs from S. enteritidis-challenged hens and 2/50 (4.0%) of eggs from S. typhimurium-challenged hens ...
June G A - - 1995
The effectiveness of selenite cystine (SC) broth, tetrathionate (TT) broth, and Rappaport-Vassiliadis (RV) medium for recovery of Salmonella spp. from 8 highly contaminated foods was determined. RV medium prepared from individual ingredients and incubated at 42 degrees and 43 degrees C was compared with 2 commercial (Difco and Oxoid) media ...
Jones G P - - 1995
1. Broilers selected on the basis of food conversion efficiency or growth rate were grown under normal (21%) or varied (19%, 23%) oxygen environments. 2. Broilers selected for food conversion efficiency had greater growth rates and superior food conversion efficiencies than broilers selected for growth rate. No interactions between broiler ...
Tassou C C - - 1995
The inhibitory effect of commercial 'pure' oleuropein was tested against Salmonella enteritidis in a coliform broth and in reconstituted milk (model food system). It was found that the inhibition of this organism in the broth was influenced by the initial inoculum size, the pH of the medium and the concentration ...
Baffone W - - 1995
The results of research on the spreading of campylobacter in the Pesaro-Urbino area carried out from 1985 to 1992 are presented. Materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and ...
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