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Pfister Christina C Department of Neurosurgery, University of Tuebingen, Hoppe-Seyler-Str.3, 72076, Tuebingen, Germany, - - 2015
There are several methods to detect apoptosis using cleaved caspase-3 and each harbors its own advantages and disadvantages. When primary cell cultures are used, the disadvantages of the standard methods can make apoptosis detection difficult due to their slow growth rate and replicative senescence, thereby limiting the available cell number ...
Vega-Naredo Ignacio I CNC - Center for Neuroscience and Cell Biology, Largo Marquês de Pombal, University of Coimbra, 3004-517, Coimbra, - - 2015
Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, ...
Colin Sophie S Université Lille 2, Lille, France; Inserm, U1011, Lille, France; Institut Pasteur de Lille, Lille, France; European Genomic Institute for Diabetes (EGID), FR 3508, Lille, - - 2014
Initiation and progression of atherosclerosis depend on local inflammation and accumulation of lipids in the vascular wall. Although many cells are involved in the development and progression of atherosclerosis, macrophages are fundamental contributors. For nearly a decade, the phenotypic heterogeneity and plasticity of macrophages has been studied. In atherosclerotic lesions, ...
Yu Lijian L 1 Department of Microbiology and Physiological Systems, University of Massachusetts Medical School , Worcester, - - 2014
We demonstrate that interferon (IFN)-β-1b induces an alternative-start transcript containing the C-terminal TLDc domain of nuclear receptor coactivator protein 7 (NCOA7), a member of the OXR family of oxidation resistance proteins. IFN-β-1b induces NCOA7-AS (alternative start) expression in peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals and multiple sclerosis ...
Xu Jiangsheng - - 2014
The applications of photodynamic therapy (PDT) are usually limited by photosensitizer's side effect and the singlet oxygen's short half-life. Herein, we demonstrate a dual-targeting (both cellular and subcellular targeting) strategy to enhance the PDT efficacy. A cationic porphyrin derivative (MitoTPP) was synthesized as the mitochondrion-targeting photosensitizer; and the dual-targeting PDT ...
Shimizu Yuri - - 2014
Apoptosis is characterized by distinct morphological and biochemical changes that occur upon activation of a family of serine proteases known as caspases. Reactive oxygen species induce apoptosis in many cell systems. Nuclear receptor subfamily 4, group A, member 1 (NR4A1) has been shown to induce apoptosis in a number of ...
Gañán-Gómez I I Department of System Biology, Unit of Biochemistry and Molecular Biology, Faculty of Medicine and Health Sciences, University of Alcalá (UAH), Carretera Madrid-Barcelona Km 33.6 s/n, 28871, Alcalá de Henares, Madrid, - - 2014
Current frontline therapies have improved overall survival in acute promyelocytic leukemia (APL) patients to exceptional rates; however, relapse is still a problem among high-risk and old patients. Therefore, the development of better and safer therapies continues to be a goal in the treatment of this disease. In the present work, ...
Wang Min M Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing, 100193, People's Republic of - - 2014
Endoplasmic reticulum (ER) stress-induced apoptosis has been suggested to contribute to myocardial ischemia-reperfusion (I/R) injury. Elatoside C is one of the major triterpenoid compounds isolated from Aralia elata that is known to be cardioprotective. However, its effects on I/R injury to cardiac myocytes have not been clarified. This study aimed ...
Zhao Xiurong X Stroke Program - Department of Neurology, University of Texas Health Science Center, Medical School at Houston, Texas, - - 2014
As a consequence of intracerebral hemorrhage (ICH), blood components enter brain parenchyma causing progressive damage to the surrounding brain. Unless hematoma is cleared, the reservoirs of blood continue to inflict injury to neurovascular structures and blunt the brain repair processes. Microglia/macrophages (MMΦ) represent the primary phagocytic system that mediates the ...
Obulesu M M Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan, - - 2014
Alzheimer's disease (AD) is a devastative neurodegenerative disorder with complex etiology. Apoptosis, a biological process that plays an essential role in normal physiology to oust a few cells and contribute to the normal growth, when impaired or influenced by various factors such as Bcl2, Bax, caspases, amyloid beta, tumor necrosis ...
Pavlides Stephanos S Manchester Breast Centre & Breakthrough Breast Cancer Research Unit, Paterson Institute for Cancer Research, School of Cancer, Enabling Sciences and Technology, Manchester Academic Health Science Centre, University of Manchester, Manchester, - - 2014
Atherosclerosis is a complex disease initiated by the vascular accumulation of lipoproteins in the sub-endothelial space, followed by the infiltration of monocytes into the arterial intima. Caveolin-1 (Cav-1) plays an essential role in the regulation of cellular cholesterol metabolism and of various signaling pathways. In order to study specifically the ...
Rožman S S Institute of Pharmacology, University of Bern, Bern CH-3010, - - 2014
Autophagy has been demonstrated to have an essential function in several cellular hematopoietic differentiation processes, for example, the differentiation of reticulocytes. To investigate the role of autophagy in neutrophil granulopoiesis, we studied neutrophils lacking autophagy-related (Atg) 5, a gene encoding a protein essential for autophagosome formation. Using Cre-recombinase mediated gene ...
Ballweg Korbinian K Helmholtz Centre Munich / University Hospital of the LMU - - 2014
Cigarette smoke is the main risk factor for chronic obstructive pulmonary disease (COPD). Exposure of cells to cigarette smoke induces an initial adaptive cellular stress response involving increased oxidative stress and induction of inflammatory signaling pathways. Exposure of mitochondria to cellular stress alters their fusion/fission dynamics. While mild stress induces ...
Liu Wenjie W Department of Anesthesiology, Xiangya Hospital, Central South University, 87 Xiangya Road, 410008, Changsha, Hunan, - - 2014
Oxidative stress, mitochondrial dysfunction and neuronal apoptosis are thought to be major contributors of Isoflurane toxicity. However, the underlying mechanisms remain largely to be determined. DJ-1, a protein that is involved in the response to various kinds of stress, has shown its neuroprotective effects. Whether DJ-1 has a neuroprotective effect ...
Lee Ju-Hye JH Department of Food and Nutrition and ‡Research Institute of Basic Science, Sunchon National University , Suncheon 540-742, Republic of - - 2014
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is currently in clinical trials as a cancer treatment due to its ability to induce apoptosis selectively in cancer cells. Nevertheless, the risk of developing resistance warrants the development of sensitizers that can overcome resistance to TRAIL. In this study, isoegomaketone (1) acted as ...
Wang Yi Y Institute of Clinical Pharmacology, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of - - 2014
The aim of the present study was to explore the role of miR-126 in palmitate-induced HUVECs apoptosis and the possible mechanisms. Palmitate inhibited miR-126 expression in HUVECs, increased reactive oxygen species (ROS) production, and induced apoptosis as determined by up-regulation of caspase-3 activity and DNA fragmentation. Overexpression of miR-126 decreased ...
Ahmadi Maryam M Antitope Ltd, Babraham Research Campus, Babraham, Cambridge, CB22 3AT, - - 2014
Determine the effect of minute quantities of sub-visible aggregates on the in vitro immunogenicity of clinically relevant protein therapeutics. Monoclonal chimeric (rituximab) and humanized (trastuzumab) antibodies were subjected to fine-tuned stress conditions to achieve low levels (<3% of total protein) of sub-visible aggregates. The effect of stimulating human dendritic cells ...
Huang Chahua C Department of Cardiology, Second affiliated hospital, Nanchang University, Nanchang, 330006, - - 2014
Bim is a potent pro-apoptotic BH3-only Bcl-2 member. However, the expression of Bim and its role in cardiac injury induced by ischemia remain unclear. H9c2 cells were subjected to a glucose and oxygen-deprived (GOD) condition in vitro, mimicking ischemia environment in vivo. GOD treatment augmented the expression of Bim and ...
He Qin Q Department of Cardiology, The Central Hospital of Wuhan, Wuhan, Hubei 430014, P.R. - - 2014
Inflammatory response and cardiomyocyte apoptosis are important processes in ventricular remodeling post‑myocardial infarction (MI) and may form the basic mechanisms in the development of chronic heart failure. The nuclear factor κB (NF‑κB) signaling pathway could promote inflammation and apoptosis and it has been demonstrated that lycopene inhibits cigarette smoke extract‑mediated ...
Dorn Gerald W GW Internal Medicine, Washington University School of Medicine - - 2014
Mitochondrial research is experiencing a renaissance in part due to the recognition that these endosymbiotic descendants of primordial protobacteria appear to be pursuing their own biological agendas. Not only is mitochondrial metabolism required to produce most of the biochemical energy that supports their eukaryotic hosts (us), but mitochondria can actively ...
Nelson Charles C Department of Cancer Biology, University of Massachusetts Medical School, 01605, Worcester, MA, - - 2014
Macro-autophagy (hereafter referred to as autophagy) delivers cytoplasmic material to the lysosome for degradation, and has been implicated in many cellular processes, including stress, infection, survival, and death. While the regulation and role that autophagy plays in stress, infection, and survival is apparent, the regulation of and role that autophagy ...
Baliu-Piqué Mariona M Department of Surgery, Technische Universität München, Munich, - - 2014
Environmental signals shape the phenotype and function of activated macrophages. Here, we show that the neuropeptide CGRP, which is released from sensory nerves, modulates the phenotype of TLR4-activated murine macrophages by enhancing expression of the regulatory macrophage markers IL-10, SPHK1 and LIGHT. In contrast, CGRP inhibits production of cytokines characteristic ...
Walsh James T JT Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, VA 22908; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22908; Graduate Program in Neuroscience, University of Virginia, Charlottesville, VA 22908, Medical Scientist Training Program, School of Medicine, University of Virginia, Charlottesville, VA - - 2014
Previous research investigating the roles of T effector (Teff) and T regulatory (Treg) cells after injury to the CNS has yielded contradictory conclusions, with both protective and destructive functions being ascribed to each of these T cell subpopulations. In this work, we study this dichotomy by examining how regulation of ...
Wang Yue - - 2014
Glucose-regulated proteins (GRP) are induced in the cancer microenvironment to promote tumor survival, metastasis and drug resistance. AST was obtained from the medicinal plant Astragalus membranaceus, which possesses anti-tumor and pro-apoptotic properties in colon cancer cells and tumor xenograft. The present study aimed to investigate the involvement of GRP in ...
Gong Li-Li LL Beijing Chao-Yang Hospital, Capital Medical University, - - 2014
Akebia saponin D (ASD) is a typical bioactive triterpenoid saponin obtained from the rhizome of Dipsacus asper Wall. Previous studies have found that ASD has a hepatoprotective effect in a mouse model. The purpose of this paper was to explore the molecular mechanism of the hepatoprotective effects of ASD on ...
Tam Eric K W EK Institute of Chemical & Engineering Sciences, Agency for Science, Technology & Research (A*STAR), 8 Biomedical Grove, Neuros #07-01, Singapore 138665 - - 2014
3-Deazaneplanocin A (DzNep) is a potential epigenetic drug for the treatment of various cancers. DzNep has been reported to deplete histone methylations, including oncogenic EZH2 complex, giving rise to epigenetic modifications that reactivate many silenced tumor suppressors in cancer cells. Despite its promise as an anticancer drug, little is known about ...
Feingold Kenneth R KR VA Medical Center, United States; - - 2014
Many of the beneficial and adverse effects of niacin are mediated via a G protein receptor, GPR109A/HCA2, which is highly expressed in adipose tissue and macrophages. Here we demonstrate that immune activation increases GPR109A/HCA2 expression. LPS, TNF, and IL-1 increase GPR109A/HCA2 expression 3-5 fold in adipose tissue. LPS also increased ...
Hirano Takao T Department of Ophthalmology, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, - - 2014
Although the transcription factor NF-κB is known to regulate cell death and survival, its precise role in cell death within the central nervous system remains unknown. We previously reported that mice with a homozygous deficiency for NF-κBp50 spontaneously develop optic neuropathy. The aim of the present study was to demonstrate ...
Sharma Ashish A AA Child & Family Research Institute, Vancouver, BC, Canada; Department of Experimental Medicine, University of British Columbia, Vancouver, BC, - - 2014
Interleukin-1β (IL-1β) production is impaired in cord blood monocytes. However, the mechanism underlying this developmental attenuation remains unclear. Here, we analyzed the extent of variability within the Toll-like receptor (TLR)/NLRP3 inflammasome pathways in human neonates. We show that immature low CD14-expressing/CD16(pos) monocytes predominate before 33 weeks of gestation, and that ...
Goeritzer Madeleine M Medical University of Graz, - - 2014
Cellular TG stores are efficiently hydrolyzed by adipose triglyceride lipase (ATGL). Its co-activator comparative gene identification-58 (CGI-58) strongly increases ATGL-mediated TG catabolism in cell culture experiments. To investigate the consequences of CGI-58 deficiency in murine macrophages, we generated mice with a targeted deletion of CGI-58 in myeloid cells (macCGI-58-/- mice). ...
Yu Jiujiu J Department of Genetics & Complex Diseases, Harvard School of Public Health, Boston, MA - - 2014
Inflammasomes are intracellular sensors that couple detection of pathogens and cellular stress to activation of Caspase-1, and consequent IL-1β and IL-18 maturation and pyroptotic cell death. Here, we show that the absent in melanoma 2 (AIM2) and nucleotide-binding oligomerization domain-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasomes trigger Caspase-1-dependent mitochondrial ...
Niethammer Philipp P Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. Electronic address: - - 2014
Reactive oxygen species (ROS) are generated as a response to cellular stress and regulate processes including cellular signaling and wound healing. In this issue of Developmental Cell, Xu and Chisholm (2014) demonstrate that mitochondrial ROS are required for proper wound healing in Caenorhabditis elegans through controlling the redox state of ...
Li Ming M Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. - - 2014
Cyclooxygenase-2 (COX-2) and its metabolite prostaglandin E2 (PGE2) can potentially affect most of the events in cancer development, including promotion of proliferation, resistance to apoptosis, angiogenesis, immune suppression and invasion. However, worldwide attention has predominantly centered on the cardiovascular toxicity of selective COX-2 inhibitors. Paeonol is a major active extract from ...
Xing Yingying Y State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People's Republic of - - 2014
Ht-2 is a novel oxaliplatin derivative previously identified in a compound screen performed by our laboratory. In the present study, we evaluated the antitumor effects of Ht-2 and investigated its underlying mechanism of action. Ht-2 exhibited anti-tumor activity and demonstrated low cytotoxicity in normal cells in vitro. The IC50 of ...
Gangoda Lahiru L Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, 3086, - - 2014
Extracellular vesicles are signaling organelles that are released by many cell types and is highly conserved in both prokaryotes and eukaryotes. Based on the mechanism of biogenesis, these membranous vesicles can be classified as exosomes, shedding microvesicles and apoptotic blebs. It is becoming clearer that these extracellular vesicles mediate signal ...
Shi Xi - - 2014
The HMGA2 protein has previously been shown as an oncoprotein, whereas ectopic expression of HMGA2 is found to induce growth arrest in primary cells. The precise mechanisms underlying this phenomenon remain to be unraveled. In this study, we determined that HMGA2 was able to induce apoptosis in WI38 primary human ...
Liu Jingbo J Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR - - 2014
Peptides derived from egg white protein are well-known for their abundant species and biological properties. The aim of this study was to investigate the anti-oxidative and anti-apoptosis effects of (Trp-Asn-Trp-Ala-Asp) WNWAD, a pentapeptide derived from egg white ovomucin pepsin hydrolysates, against the oxidative stress induced by H2O2 in HEK-293 cells. ...
Zhao Engui E HKUST Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park Nanshan, Shenzhen 518057, China. - - 2014
We report a dual functional aggregation-enhanced emission (AEE) molecule, TPE-IQ, which could target and illuminate mitochondria in both live and fixed cells with superb selectivity and high signal-to-noise ratio. More intriguingly, TPE-IQ can serve as a photosensitizer to generate reactive oxygen species (ROS) in the mitochondria region to induce cell ...
Iwaniuk A A Department of Immunology, Medical University of Białystok, Poland - - 2014
N-nitrosodimethylamine (NDMA) is a xenobiotic widespread in human environment capable of regulating the lifespan of immune cells. In this study, we examined the roles of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/death receptor 5 (DR5) complex and the Fas molecule in the induction of the extrinsic apoptosis pathway in human ...
Qin Yu Y Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, China. Electronic address: - - 2014
MicroRNA-125b (miR-125b) has been implicated in a variety of diseases as either repressors or promoters, and plays crucial roles in many cellular processes such as cell differentiation, proliferation and apoptosis. Age-related cataract has become one of the most serious problems facing the aging population in the world. The purpose of ...
Bononi Angela A Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, - - 2014
The tumor suppressor PTEN is a key regulator of a plethora of cellular processes that are crucial in cancer development. Through its lipid phosphatase activity PTEN suppresses the PI3K/AKT pathway to govern cell proliferation, growth, migration, energy metabolism and death. The repertoire of roles fulfilled by PTEN has recently been ...
Jiang Qiaoling Q Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of - - 2014
To investigate whether endoplasmic reticulum (ER) stress participates in the induction of apoptosis in HepG2 cells exposed to high glucose and explore its probable mechanism. A series of experiments were performed following HepG2 cells treated with different concentrations of glucose for 48 h. The apoptosis was detected by means of Hoechst ...
Haas Benjamin B Université Paris-Est, UMR_S955, UPEC, F-94000, France; INSERM, U955, Equipe 03, F-94000, - - 2014
We hypothesized that O2 tension influences the redox state and the immunomodulatory responses of inflammatory cells to dimethyl fumarate (DMF), an activator of the nuclear factor Nrf2 that controls antioxidant genes expression. This concept was investigated in macrophages permanently cultured at either physiological (5% O2 ) or atmospheric (20% O2 ...
Gutierrez Elaine E University of Sydney, - - 2014
Autophagy functions as a survival mechanism during cellular stress and contributes to resistance against anti-cancer agents. The selective anti-tumor and anti-metastatic chelator, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT), causes lysosomal membrane permeabilization and cell death. Considering the integral role of lysosomes in autophagy and cell death, it was important to assess the effect ...
Morucci Gabriele G Departments of aExperimental and Clinical Medicine bClinical and Biomedical Experimental Sciences cNeurosciences, Psychology, Drug Research and Child Health, Section of Pharmacology and Toxicology, University of Firenze, Firenze, Italy dImmuno Biotech Ltd, St. Peter Port, Guernsey, Channel Islands, - - 2014
Oxaliplatin-based regimens are effective in metastasized advanced cancers. However, a major limitation to their widespread use is represented by neurotoxicity that leads to peripheral neuropathy. In this study we evaluated the roles of a proven immunotherapeutic agent [Gc-protein-derived macrophage activating factor (GcMAF)] in preventing or decreasing oxaliplatin-induced neuronal damage and ...
Zhou Qian Q Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, P. R. China; Department of Biochemistry and Molecular Biology, and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932, - - 2014
Rotenone, a common pesticide and inhibitor of mitochondrial complex I, induces loss of dopaminergic neurons and consequential aspects of Parkinson's disease (PD). However, the exact mechanism of rotenone neurotoxicity is not fully elucidated. Here, we show that rotenone induced reactive oxygen species (ROS), leading to apoptotic cell death in PC12 ...
Li Mingying M The Fourth Department of Tuberculosis, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. - - 2014
The Mycobacterium tuberculosis 19-kDa lipoprotein (P19) is both cell wall-associated and secreted and is a candidate virulence factor that could cause the apoptosis of human macrophages infected with M. tuberculosis. P19 induces TLR2 activation, resulting in the upregulation of death receptors and ligands, followed by a death-receptor signaling cascade. The mechanisms ...
Hu Rong R Department of Hematology, Shengjing Hospital, China Medical University, Shenyang, 110004, Liaoning, - - 2014
The present study was designed to explore the effects of low-toxicity Embelin on TRAIL-induced apoptosis and its possible mechanism in human leukemia cells. Our study showed that low-toxicity Embelin enhanced TRAIL-induced apoptosis through DR4 and DR5 upregulation and caspase activation in HL-60 cells. Pan-caspase inhibitor Z-VAD-FMK inhibited cell apoptosis induced ...
Tanaka Shingo S Department of Neurosurgery, Division of Neuroscience, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, - - 2014
The Notch signal regulates both cell viability and apoptosis, and maintains stemness of various cancers including glioblastoma (GBM). Although Notch signal inhibition may be an effective strategy in treating GBM initiating cells (GICs), its applicability to the different subtypes of GBM remains unclear. Here, we analyzed the effectiveness of MRK003, ...
Shi Guojun G G Shi, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrinology and Metabolism Rui-jin Hospital, Shanghai, - - 2014
Recent reports have highlighted the roles of free fatty acid receptor 2 (FFAR2) in the regulation of metabolic and inflammatory processes. However, the potential function of FFAR2 in type 1 diabetes (T1D) remains unexplored. Our results showed that the mRNA level of FFAR2 was up-regulated in peripheral blood mononuclear cells ...
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