Search Results
Results 1 - 50 of 1790
1 2 3 4 5 6 7 8 9 10 >
Pfister Christina C Department of Neurosurgery, University of Tuebingen, Hoppe-Seyler-Str.3, 72076, Tuebingen, Germany, - - 2015
There are several methods to detect apoptosis using cleaved caspase-3 and each harbors its own advantages and disadvantages. When primary cell cultures are used, the disadvantages of the standard methods can make apoptosis detection difficult due to their slow growth rate and replicative senescence, thereby limiting the available cell number ...
Vega-Naredo Ignacio I CNC - Center for Neuroscience and Cell Biology, Largo Marquês de Pombal, University of Coimbra, 3004-517, Coimbra, - - 2015
Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, ...
Colin Sophie S Université Lille 2, Lille, France; Inserm, U1011, Lille, France; Institut Pasteur de Lille, Lille, France; European Genomic Institute for Diabetes (EGID), FR 3508, Lille, - - 2014
Initiation and progression of atherosclerosis depend on local inflammation and accumulation of lipids in the vascular wall. Although many cells are involved in the development and progression of atherosclerosis, macrophages are fundamental contributors. For nearly a decade, the phenotypic heterogeneity and plasticity of macrophages has been studied. In atherosclerotic lesions, ...
Chen Haibo H Department of Cardiology, The First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, 23 Youzheng Street, Harbin, 150001, Heilongjiang, - - 2014
Ultrasound combined with endogenous protoporphyrin IX derived from 5-aminolevulinic acid (ALA-SDT) is known to induce apoptosis in multiple cancer cells and macrophages. Persistent retention of macrophages in the plaque has been implicated in the pathophysiology and progression of atherosclerosis. Here we investigated the effects of inhibition of voltage-dependent anion channel ...
Ren Weiying W Department of Geriatrics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, - - 2014
Toll-like receptor 4 (TLR4) and myeloid differentiation protein 2 (MD-2) are the main lipopolysaccharide (LPS) binding receptors that respond to inflammatory stimuli and mediate NF-kappa B (NF-κB) signaling pathway in macrophages. We have previously shown that plasminogen activator inhibitor-1 (PAI-1) deletion increased lung injury induced by intratracheal instillation of LPS ...
Liu Bao-Xin-Zi - - 2014
Colorectal cancer has become one of the leading cause of cancer morbidity and mortality throughout world. Hederagenin, a derivative of oleanolic acid isolated from the leaves of ivy (Hedera helix L.), has been shown to have potential anti-tumor activity. The study was conducted to evaluate whether hederagenin could induce apoptosis ...
Chen Chengzhi C Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu, Sichuan, People's Republic of - - 2014
Arsenic trioxide (As2 O3 ) is commonly used to treat acute promyelocytic leukemia and solid tumors. However, the clinical application of the agent is limited by its cyto- and genotoxic effects on normal cells. Thus, relief of As2 O3 toxicity in normal cells is essentially necessary for improvement of As2 ...
Bellner Lars L Department of Pharmacology, - - 2014
Heme oxygenase (HO)-2 deficiency impairs wound healing and exacerbates inflammation following injury. We examine the impact of HO-2 deficiency on macrophage function and the contribution of macrophage HO-2 to inflammatory and repair responses to injury. Corneal epithelial debridement was performed in control and macrophage-depleted HO-2(-/-) and wild-type (WT) mice and ...
Yin Wenbin W *Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatrics, Qilu Hospital of Shandong University, Jinan, China † Institute of Basic Science, Medical Science Academy of Shandong, Jinan, China †† Division of Cardiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of - - 2014
Elevated level of glycated low-density lipoproteins (glyLDL) is believed to contribute to endothelial dysfunction, which is involved in the pathogenesis and acceleration of diabetic vascular diseases. Grape seed procyanidin B2 (GSPB2) has been reported to possess protective effects against endothelial dysfunction. However, the underlying mechanism remains unclear. Prohibitin (PHB) is ...
Kuebler Ulrike U Department of Clinical Psychology and Psychotherapy, University of Zurich, Zurich, - - 2014
Anticipatory cognitive stress appraisal (ACSA) can affect the stress-induced release of stress hormones, which, in turn, can modulate microbicidal potential of macrophages. This study examines whether ACSA modulates wound-induced activation of macrophage microbicidal potential in 22 acutely stressed compared to 17 nonstressed healthy men. After catheter-induced wound infliction and completing ...
Li Bin B Department of MICU, The First Affiliated Hospital, Sun Yat-Sen University, #58Zhongshan second Rd, Guangzhou 510080, - - 2014
Alveolar macrophages (AMs) undergo increased apoptosis during sepsis-induced acute respiratory distress syndrome (ARDS). Ghrelin exhibits an antiapoptotic effect in several cell types and protects against sepsis-induced ARDS in rats; however, the molecular mechanisms underlying this antiapoptotic effect remain poorly understood. In this study, we first examined the antiapoptotic effect of ...
Hutt Karla J KJ K Hutt, Ovarian Biology, MIMR-PHI Institute, Clayton, Australia - - 2014
BH3-only proteins are pro-apoptotic members of the BCL-2 family that play pivotal roles in embryonic development, tissue homeostasis and immunity by triggering cell death though the intrinsic apoptosis pathway. Recent in vitro and in vivo studies have demonstrated that BH3-only proteins are also essential mediators of apoptosis within the ovary ...
Zhang Bo B Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Pharmacology Department, School of Pharmacy, Shihezi University, - - 2014
Among the various cancer cell lines, HeLa cells were found to be sensitive to pterostilbene (Pte), a compound that is enriched in small fruits such as grapes and berries. However, the mechanism involved in the cytotoxicity of Pte has not been fully characterized. Using biochemical and free radical biological experiments ...
Ooi Yin Yin YY Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, - - 2014
Background/Aim: Microglia, the resident macrophages in the central nervous system, secrete various proinflammatory cytokines and undergo proliferation upon activation in various neurodegenerative diseases. Activation of microglia has been implicated in exacerbation of various neurodegenerative diseases. Recently, it has been proposed that mesenchymal stem cells (MSC) have immunosuppressive properties and the ...
Yu Lijian L 1 Department of Microbiology and Physiological Systems, University of Massachusetts Medical School , Worcester, - - 2014
We demonstrate that interferon (IFN)-β-1b induces an alternative-start transcript containing the C-terminal TLDc domain of nuclear receptor coactivator protein 7 (NCOA7), a member of the OXR family of oxidation resistance proteins. IFN-β-1b induces NCOA7-AS (alternative start) expression in peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals and multiple sclerosis ...
Xu Jiangsheng - - 2014
The applications of photodynamic therapy (PDT) are usually limited by photosensitizer's side effect and the singlet oxygen's short half-life. Herein, we demonstrate a dual-targeting (both cellular and subcellular targeting) strategy to enhance the PDT efficacy. A cationic porphyrin derivative (MitoTPP) was synthesized as the mitochondrion-targeting photosensitizer; and the dual-targeting PDT ...
Shimizu Yuri - - 2014
Apoptosis is characterized by distinct morphological and biochemical changes that occur upon activation of a family of serine proteases known as caspases. Reactive oxygen species induce apoptosis in many cell systems. Nuclear receptor subfamily 4, group A, member 1 (NR4A1) has been shown to induce apoptosis in a number of ...
Gañán-Gómez I I Department of System Biology, Unit of Biochemistry and Molecular Biology, Faculty of Medicine and Health Sciences, University of Alcalá (UAH), Carretera Madrid-Barcelona Km 33.6 s/n, 28871, Alcalá de Henares, Madrid, - - 2014
Current frontline therapies have improved overall survival in acute promyelocytic leukemia (APL) patients to exceptional rates; however, relapse is still a problem among high-risk and old patients. Therefore, the development of better and safer therapies continues to be a goal in the treatment of this disease. In the present work, ...
Wang Min M Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing, 100193, People's Republic of - - 2014
Endoplasmic reticulum (ER) stress-induced apoptosis has been suggested to contribute to myocardial ischemia-reperfusion (I/R) injury. Elatoside C is one of the major triterpenoid compounds isolated from Aralia elata that is known to be cardioprotective. However, its effects on I/R injury to cardiac myocytes have not been clarified. This study aimed ...
Zhao Xiurong X Stroke Program - Department of Neurology, University of Texas Health Science Center, Medical School at Houston, Texas, - - 2014
As a consequence of intracerebral hemorrhage (ICH), blood components enter brain parenchyma causing progressive damage to the surrounding brain. Unless hematoma is cleared, the reservoirs of blood continue to inflict injury to neurovascular structures and blunt the brain repair processes. Microglia/macrophages (MMΦ) represent the primary phagocytic system that mediates the ...
Obulesu M M Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan, - - 2014
Alzheimer's disease (AD) is a devastative neurodegenerative disorder with complex etiology. Apoptosis, a biological process that plays an essential role in normal physiology to oust a few cells and contribute to the normal growth, when impaired or influenced by various factors such as Bcl2, Bax, caspases, amyloid beta, tumor necrosis ...
Pavlides Stephanos S Manchester Breast Centre & Breakthrough Breast Cancer Research Unit, Paterson Institute for Cancer Research, School of Cancer, Enabling Sciences and Technology, Manchester Academic Health Science Centre, University of Manchester, Manchester, - - 2014
Atherosclerosis is a complex disease initiated by the vascular accumulation of lipoproteins in the sub-endothelial space, followed by the infiltration of monocytes into the arterial intima. Caveolin-1 (Cav-1) plays an essential role in the regulation of cellular cholesterol metabolism and of various signaling pathways. In order to study specifically the ...
Rožman S S Institute of Pharmacology, University of Bern, Bern CH-3010, - - 2014
Autophagy has been demonstrated to have an essential function in several cellular hematopoietic differentiation processes, for example, the differentiation of reticulocytes. To investigate the role of autophagy in neutrophil granulopoiesis, we studied neutrophils lacking autophagy-related (Atg) 5, a gene encoding a protein essential for autophagosome formation. Using Cre-recombinase mediated gene ...
Ballweg Korbinian K Helmholtz Centre Munich / University Hospital of the LMU - - 2014
Cigarette smoke is the main risk factor for chronic obstructive pulmonary disease (COPD). Exposure of cells to cigarette smoke induces an initial adaptive cellular stress response involving increased oxidative stress and induction of inflammatory signaling pathways. Exposure of mitochondria to cellular stress alters their fusion/fission dynamics. While mild stress induces ...
Liu Wenjie W Department of Anesthesiology, Xiangya Hospital, Central South University, 87 Xiangya Road, 410008, Changsha, Hunan, - - 2014
Oxidative stress, mitochondrial dysfunction and neuronal apoptosis are thought to be major contributors of Isoflurane toxicity. However, the underlying mechanisms remain largely to be determined. DJ-1, a protein that is involved in the response to various kinds of stress, has shown its neuroprotective effects. Whether DJ-1 has a neuroprotective effect ...
Lee Ju-Hye JH Department of Food and Nutrition and ‡Research Institute of Basic Science, Sunchon National University , Suncheon 540-742, Republic of - - 2014
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is currently in clinical trials as a cancer treatment due to its ability to induce apoptosis selectively in cancer cells. Nevertheless, the risk of developing resistance warrants the development of sensitizers that can overcome resistance to TRAIL. In this study, isoegomaketone (1) acted as ...
Wang Yi Y Institute of Clinical Pharmacology, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of - - 2014
The aim of the present study was to explore the role of miR-126 in palmitate-induced HUVECs apoptosis and the possible mechanisms. Palmitate inhibited miR-126 expression in HUVECs, increased reactive oxygen species (ROS) production, and induced apoptosis as determined by up-regulation of caspase-3 activity and DNA fragmentation. Overexpression of miR-126 decreased ...
Ahmadi Maryam M Antitope Ltd, Babraham Research Campus, Babraham, Cambridge, CB22 3AT, - - 2014
Determine the effect of minute quantities of sub-visible aggregates on the in vitro immunogenicity of clinically relevant protein therapeutics. Monoclonal chimeric (rituximab) and humanized (trastuzumab) antibodies were subjected to fine-tuned stress conditions to achieve low levels (<3% of total protein) of sub-visible aggregates. The effect of stimulating human dendritic cells ...
Huang Chahua C Department of Cardiology, Second affiliated hospital, Nanchang University, Nanchang, 330006, - - 2014
Bim is a potent pro-apoptotic BH3-only Bcl-2 member. However, the expression of Bim and its role in cardiac injury induced by ischemia remain unclear. H9c2 cells were subjected to a glucose and oxygen-deprived (GOD) condition in vitro, mimicking ischemia environment in vivo. GOD treatment augmented the expression of Bim and ...
He Qin Q Department of Cardiology, The Central Hospital of Wuhan, Wuhan, Hubei 430014, P.R. - - 2014
Inflammatory response and cardiomyocyte apoptosis are important processes in ventricular remodeling post‑myocardial infarction (MI) and may form the basic mechanisms in the development of chronic heart failure. The nuclear factor κB (NF‑κB) signaling pathway could promote inflammation and apoptosis and it has been demonstrated that lycopene inhibits cigarette smoke extract‑mediated ...
Dorn Gerald W GW Internal Medicine, Washington University School of Medicine - - 2014
Mitochondrial research is experiencing a renaissance in part due to the recognition that these endosymbiotic descendants of primordial protobacteria appear to be pursuing their own biological agendas. Not only is mitochondrial metabolism required to produce most of the biochemical energy that supports their eukaryotic hosts (us), but mitochondria can actively ...
Nelson Charles C Department of Cancer Biology, University of Massachusetts Medical School, 01605, Worcester, MA, - - 2014
Macro-autophagy (hereafter referred to as autophagy) delivers cytoplasmic material to the lysosome for degradation, and has been implicated in many cellular processes, including stress, infection, survival, and death. While the regulation and role that autophagy plays in stress, infection, and survival is apparent, the regulation of and role that autophagy ...
Baliu-Piqué Mariona M Department of Surgery, Technische Universität München, Munich, - - 2014
Environmental signals shape the phenotype and function of activated macrophages. Here, we show that the neuropeptide CGRP, which is released from sensory nerves, modulates the phenotype of TLR4-activated murine macrophages by enhancing expression of the regulatory macrophage markers IL-10, SPHK1 and LIGHT. In contrast, CGRP inhibits production of cytokines characteristic ...
Walsh James T JT Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, VA 22908; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22908; Graduate Program in Neuroscience, University of Virginia, Charlottesville, VA 22908, Medical Scientist Training Program, School of Medicine, University of Virginia, Charlottesville, VA - - 2014
Previous research investigating the roles of T effector (Teff) and T regulatory (Treg) cells after injury to the CNS has yielded contradictory conclusions, with both protective and destructive functions being ascribed to each of these T cell subpopulations. In this work, we study this dichotomy by examining how regulation of ...
Wang Yue - - 2014
Glucose-regulated proteins (GRP) are induced in the cancer microenvironment to promote tumor survival, metastasis and drug resistance. AST was obtained from the medicinal plant Astragalus membranaceus, which possesses anti-tumor and pro-apoptotic properties in colon cancer cells and tumor xenograft. The present study aimed to investigate the involvement of GRP in ...
Gong Li-Li LL Beijing Chao-Yang Hospital, Capital Medical University, - - 2014
Akebia saponin D (ASD) is a typical bioactive triterpenoid saponin obtained from the rhizome of Dipsacus asper Wall. Previous studies have found that ASD has a hepatoprotective effect in a mouse model. The purpose of this paper was to explore the molecular mechanism of the hepatoprotective effects of ASD on ...
Tam Eric K W EK Institute of Chemical & Engineering Sciences, Agency for Science, Technology & Research (A*STAR), 8 Biomedical Grove, Neuros #07-01, Singapore 138665 - - 2014
3-Deazaneplanocin A (DzNep) is a potential epigenetic drug for the treatment of various cancers. DzNep has been reported to deplete histone methylations, including oncogenic EZH2 complex, giving rise to epigenetic modifications that reactivate many silenced tumor suppressors in cancer cells. Despite its promise as an anticancer drug, little is known about ...
Feingold Kenneth R KR VA Medical Center, United States; - - 2014
Many of the beneficial and adverse effects of niacin are mediated via a G protein receptor, GPR109A/HCA2, which is highly expressed in adipose tissue and macrophages. Here we demonstrate that immune activation increases GPR109A/HCA2 expression. LPS, TNF, and IL-1 increase GPR109A/HCA2 expression 3-5 fold in adipose tissue. LPS also increased ...
Hirano Takao T Department of Ophthalmology, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, - - 2014
Although the transcription factor NF-κB is known to regulate cell death and survival, its precise role in cell death within the central nervous system remains unknown. We previously reported that mice with a homozygous deficiency for NF-κBp50 spontaneously develop optic neuropathy. The aim of the present study was to demonstrate ...
Sharma Ashish A AA Child & Family Research Institute, Vancouver, BC, Canada; Department of Experimental Medicine, University of British Columbia, Vancouver, BC, - - 2014
Interleukin-1β (IL-1β) production is impaired in cord blood monocytes. However, the mechanism underlying this developmental attenuation remains unclear. Here, we analyzed the extent of variability within the Toll-like receptor (TLR)/NLRP3 inflammasome pathways in human neonates. We show that immature low CD14-expressing/CD16(pos) monocytes predominate before 33 weeks of gestation, and that ...
Goeritzer Madeleine M Medical University of Graz, - - 2014
Cellular TG stores are efficiently hydrolyzed by adipose triglyceride lipase (ATGL). Its co-activator comparative gene identification-58 (CGI-58) strongly increases ATGL-mediated TG catabolism in cell culture experiments. To investigate the consequences of CGI-58 deficiency in murine macrophages, we generated mice with a targeted deletion of CGI-58 in myeloid cells (macCGI-58-/- mice). ...
Yu Jiujiu J Department of Genetics & Complex Diseases, Harvard School of Public Health, Boston, MA - - 2014
Inflammasomes are intracellular sensors that couple detection of pathogens and cellular stress to activation of Caspase-1, and consequent IL-1β and IL-18 maturation and pyroptotic cell death. Here, we show that the absent in melanoma 2 (AIM2) and nucleotide-binding oligomerization domain-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasomes trigger Caspase-1-dependent mitochondrial ...
Niethammer Philipp P Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. Electronic address: - - 2014
Reactive oxygen species (ROS) are generated as a response to cellular stress and regulate processes including cellular signaling and wound healing. In this issue of Developmental Cell, Xu and Chisholm (2014) demonstrate that mitochondrial ROS are required for proper wound healing in Caenorhabditis elegans through controlling the redox state of ...
Li Ming M Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. - - 2014
Cyclooxygenase-2 (COX-2) and its metabolite prostaglandin E2 (PGE2) can potentially affect most of the events in cancer development, including promotion of proliferation, resistance to apoptosis, angiogenesis, immune suppression and invasion. However, worldwide attention has predominantly centered on the cardiovascular toxicity of selective COX-2 inhibitors. Paeonol is a major active extract from ...
Xing Yingying Y State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People's Republic of - - 2014
Ht-2 is a novel oxaliplatin derivative previously identified in a compound screen performed by our laboratory. In the present study, we evaluated the antitumor effects of Ht-2 and investigated its underlying mechanism of action. Ht-2 exhibited anti-tumor activity and demonstrated low cytotoxicity in normal cells in vitro. The IC50 of ...
Gangoda Lahiru L Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, 3086, - - 2014
Extracellular vesicles are signaling organelles that are released by many cell types and is highly conserved in both prokaryotes and eukaryotes. Based on the mechanism of biogenesis, these membranous vesicles can be classified as exosomes, shedding microvesicles and apoptotic blebs. It is becoming clearer that these extracellular vesicles mediate signal ...
Shi Xi - - 2014
The HMGA2 protein has previously been shown as an oncoprotein, whereas ectopic expression of HMGA2 is found to induce growth arrest in primary cells. The precise mechanisms underlying this phenomenon remain to be unraveled. In this study, we determined that HMGA2 was able to induce apoptosis in WI38 primary human ...
Liu Jingbo J Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR - - 2014
Peptides derived from egg white protein are well-known for their abundant species and biological properties. The aim of this study was to investigate the anti-oxidative and anti-apoptosis effects of (Trp-Asn-Trp-Ala-Asp) WNWAD, a pentapeptide derived from egg white ovomucin pepsin hydrolysates, against the oxidative stress induced by H2O2 in HEK-293 cells. ...
Zhao Engui E HKUST Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park Nanshan, Shenzhen 518057, China. - - 2014
We report a dual functional aggregation-enhanced emission (AEE) molecule, TPE-IQ, which could target and illuminate mitochondria in both live and fixed cells with superb selectivity and high signal-to-noise ratio. More intriguingly, TPE-IQ can serve as a photosensitizer to generate reactive oxygen species (ROS) in the mitochondria region to induce cell ...
Iwaniuk A A Department of Immunology, Medical University of Białystok, Poland - - 2014
N-nitrosodimethylamine (NDMA) is a xenobiotic widespread in human environment capable of regulating the lifespan of immune cells. In this study, we examined the roles of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/death receptor 5 (DR5) complex and the Fas molecule in the induction of the extrinsic apoptosis pathway in human ...
1 2 3 4 5 6 7 8 9 10 >