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Pfister Christina C Department of Neurosurgery, University of Tuebingen, Hoppe-Seyler-Str.3, 72076, Tuebingen, Germany, - - 2015
There are several methods to detect apoptosis using cleaved caspase-3 and each harbors its own advantages and disadvantages. When primary cell cultures are used, the disadvantages of the standard methods can make apoptosis detection difficult due to their slow growth rate and replicative senescence, thereby limiting the available cell number ...
Vega-Naredo Ignacio I CNC - Center for Neuroscience and Cell Biology, Largo Marquês de Pombal, University of Coimbra, 3004-517, Coimbra, - - 2015
Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by p53 and results in the release of pro-apoptotic factors to the cytosol, ...
Melo Fabio R FR Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Box 7011, 750 07, Uppsala, - - 2015
Mast cells (MCs) have detrimental functions in the context of numerous pathologies, and regimens aimed at neutralizing MCs or individual MC products can thus be of therapeutic value. One way to target MCs in disease is to selectively induce MC apoptosis, but there is so far no agent available that ...
Souza Jeanderson Pereira JP a Toxicological Genetics Laboratory, Department of Biological Sciences , Feira de Santana State University , Feira de Santana , Bahia , - - 2015
The aim of this study was to evaluate the potential of the androgenic anabolic steroids (AAS) for inducing chromosome damage, apoptosis, and necrosis, using the micronucleus test on exfoliated cells from the oral mucosa of AAS users. The sample consisted of 55 male individuals, practitioners of physical exercise divided into ...
Canu Nadia N Department of System Medicine, University of "Tor Vergata" Rome, Via Montpellier 1, Rome, 00137, Italy, - - 2015
Double-stranded RNARNA -mediated interferenceRNA-mediated interference (RNAiRNAi ) is a new simple and fast research tool for shutting down genesgenes and characterizes function of their respective proteinsproteins . Many strategies for design and delivery of siRNAsiRNA to target cells are available. Here, we describe the use of lentiviral short hairpinhairpin RNAlentiviral ...
Tseng Chia-Yi CY Department of Biomedical Engineering, College of Engineering, Chung Yuan Christian University, 200 Chung Pei Road, Chungli City, 32023, Taoyuan, - - 2014
Previous studies suggest a direct correlation between exposure to diesel exhaust particles (DEP) and the onset of vascular permeability, presumably through the disruption of the adherens junctions. This would lead to deleterious effects on vasculature, such as acute myocardial infarction and atherosclerosis. Although the mechanism remains unclear, we demonstrate DEP-induced ...
Yuntao Fang F Department of Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, - - 2014
Autophagy is an evolutionarily conserved process in which cytoplasmic proteins and organelles are degraded and recycled for reuse. There are numerous reports on the role of autophagy in cell growth and death; however, the role of autophagy in methylmercury (MeHg)-induced neurotoxicity has yet to be identified. We studied the role ...
Kim Nak Hyun NH Korea - - 2014
Heat shock proteins (HSPs) function as molecular chaperones and are essential for the maintenance and/or restoration of protein homeostasis. The Xanthomonas type III effector AvrBsT induces hypersensitive cell death in pepper (Capsicum annuum). Here, we report the identification of the pepper CaHSP70a as an AvrBsT-interacting protein. Bimolecular fluorescence complementation and ...
Nakka Venkata Prasuja VP Department of Neurological Surgery, School of Medicine and Public Health, University of Wisconsin, Madison, WI, 53792, - - 2014
Endoplasmic reticulum (ER) stress induces a variety of neuronal cell death pathways that play a critical role in the pathophysiology of stroke. ER stress occurs when unfolded/misfolded proteins accumulate and the folding capacity of ER chaperones exceeds the capacity of ER lumen to facilitate their disposal. As a consequence, a ...
Liao Wei-Ting WT Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, - - 2014
Cyclic GMP-dependent protein kinase II (cGKII; PRKG2) phosphorylates a variety of biological targets and has been identified as a gout-susceptible gene. However, the regulatory role of cGKII in triggering gout disease has yet to be clarified. Thus, we plan to explore the specific function of cGKII in macrophages related to ...
Ismail Norsharina - - 2014
Apoptosis is often the end result of oxidative damage to neurons. Due to shared pathways between oxidative stress, apoptosis and antioxidant defence systems, an oxidative insult could end up causing cellular apoptosis or survival depending on the severity of the insult and cellular responses. Plant bioresources have received close attention ...
Liu Y Y 1] Department of Radiation Oncology, School of Medicine University of North Carolina at Chapel Hill, Chapel Hill, NC, USA [2] Lineberger Comprehensive Cancer Center, School of Medicine University of North Carolina at Chapel Hill, Chapel Hill, NC, USA [3] Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, - - 2014
Mitochondrial outer membrane permeabilization (MOMP) is a critical control point during apoptosis that results in the release of pro-apoptotic mitochondrial contents such as cytochrome c. MOMP is largely controlled by Bcl-2 family proteins such as Bax, which under various apoptotic stresses becomes activated and oligomerizes on the outer mitochondrial membrane. ...
Musto A A 1] Department of Molecular Medicine and Medical Biotechnology, University of Naples 'Federico II', Via Sergio Pansini 5, Naples, Italy [2] Ceinge Biotecnologie Avanzate, Via Gaetano Salvatore 486, Naples, - - 2014
Numerous studies have indicated that BMP4 signaling is involved in the regulation of the early steps of development. In mouse embryonic stem cells (ESCs), BMP4 is crucial to sustain pluripotency and blocks differentiation towards neural fate. Here, through a systematic analysis of miRNAs in ESCs, we establish that BMP4 signaling ...
Nam Yoon Jeong YJ Department of Pharmacology, College of Medicine, and the BK21plus Skin Barrier Network Human Resources Development Team, Chung-Ang University, Seoul, 156-756, South - - 2014
The proteasomal dysfunction and mitochondrial impairment has been implicated in neuronal degeneration. Taxifolin has antioxidant and anti-inflammatory effects. However, the effect of taxifolin on the neuronal cell death induced by proteasome inhibition has not been studied. Therefore, in the respect of cell death process, we assessed the effect of taxifolin ...
Du Jianfeng J Department of Surgery, Boston University Medical School, Boston University, Roger Williams Medical Center, Providence, - - 2014
Histone deacetylases (HDACs) play a crucial role in the regulation of gene expression through remodeling of chromatin structures. However, the molecular mechanisms involved in this event remain unknown. In this study, we sought to examine whether HDAC inhibition-mediated protective effects involved HDAC4 sumoylation, degradation, and the proteasome pathway. Isolated neonatal ...
Sachita Khadka K Department of Oral Pathology, School of Dentistry, Institute of Biodegradable Material, Institute of Oral Bioscience, Chonbuk National University, Jeonju, - - 2014
YM155 is a small-molecule pro-apoptotic agent which has shown to inhibit survivin expression and induce apoptosis in various cancer cells. In this study, we investigated the function and molecular mechanism of YM155 in human oral cancer cells. The apoptotic effects and related signaling pathways of YM155 were evaluated using trypan blue ...
Xu Danqing D School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. - - 2014
Natural compounds from medicinal plants are important resources for drug development. Active compounds targeting apoptosis and autophagy are candidates for anti-cancer drugs. In this study, we collected Garcinia species from China and extracted them into water or ethanol fractions. Then, we performed a functional screen in search of novel apoptosis ...
Ck Hind H Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton, Faculty of Medicine, Southampton General Hospital, Southampton, SO16 6YD, - - 2014
Bfl-1 is a pro-survival Bcl-2 family member overexpressed in a subset of chemoresistant tumours, including melanoma. Here, we characterised the expression and regulation of Bfl-1 in normal and malignant melanocytes and determined its role in protecting these cells from chemotherapy-induced apoptosis. Bfl-1 was mitochondrially resident in both resting and apoptotic ...
Mao Beibei B State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, - - 2014
Co-ordination of cell proliferation, differentiation, and apoptosis maintains tissue development and homeostasis under normal or stress conditions. Recently, the highly conserved Hippo signaling pathway, discovered in Drosophila melanogaster and mammalian system, has been implicated as a key regulator of organ size control. Importantly, emerging evidence suggests that Hippo pathway is ...
Lan Bei B Key Laboratory of Microbial Functional Genomics of Ministry of Education, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, PR China; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, PR - - 2014
Parthenolide (PTL) is a sesquiterpene lactone isolated from feverfew and exhibits potent antitumor activity against various cancers. Many studies indicate that PTL treatment leads to apoptosis, however, the mechanism has not been defined. Here, we observed that cells underwent autophagy shortly after PTL treatment. Inhibition of autophagy by knocking out ...
Yeh Cheng-Chang CC School of Dentistry, College of Medicine and Department of Dentistry, National Taiwan University Hospital, National Taiwan University Medical Center, 1 Chang-Te Street, Taipei, 10048, - - 2014
Triethylene glycol dimethacrylate (TEGDMA) is a common component of resin-based dental composites and endodontic sealers. TEGDMA induces apoptosis in several types of cells. However, the mechanisms are not completely understood. The aim of this study was to investigate the mechanisms underlying TEGDMA-induced apoptosis in human embryonic palatal mesenchymal (HEPM) pre-osteoblasts ...
Das Sushmita S Department of Microbiology, All-India Institute of Medical Sciences, Patna, India; - - 2014
Regulation of macrophage PCD plays an important role in pathogenesis of leishmaniasis. However, the precise involvement of any parasite molecule in this process remains uncertain. In the current study, in silico wide analysis demonstrated that genes in the Leishmania donovani genome are highly enriched for CpG motifs, with sequence frequency ...
Fontana Mary F MF Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA 94143; - - 2014
Activated macrophages are crucial for restriction of microbial infection but may also promote inflammatory pathology in a wide range of both infectious and sterile conditions. The pathways that regulate macrophage activation are therefore of great interest. Recent studies in silico have putatively identified key transcription factors that may control macrophage ...
Ramakrishna Suresh S Department of Biomedical Science, CHA University, Bundang CHA Hospital, Gyeonggi-Do 463-400, Republic of - - 2014
The modification of proteins through post-translation and degradation by the ubiquitin-proteasome system plays a pivotal role in a broad array of biological processes. Reversal of this process by deubiquitination is a central step in the maintenance and regulation of cellular homeostasis. It now appears that the regulation of ubiquitin pathways ...
Kang Naixing N Department of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, - - 2014
Cigarette smoke extract (CSE) induces apoptosis and inflammation, but the mechanism is unknown. Arginine methyltransferase (PRMT6) catalyzes the asymmetric di-methylation of histone H3 arginine 2 (H3R2me2a) to control global level transcription. We hypothesized that PRMT6 mediates CSE induced apoptosis and inflammation through H3R2me2a. The apoptosis after CSE treatment in human ...
Ai Zhibing Z Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. - - 2014
The natural product resveratrol possesses diverse biological activities, including anti‑inflammatory, anti‑oxidant, anti‑cancer and anti‑aging effects in multiple organisms. The neuroprotective role of resveratrol has recently been reported in a cell model of amyloid (A)β(25‑35)‑induced neurotoxic injury using PC12 cells. However, the pathomechanism by which resveratrol inhibits neuronal apoptosis has remained to be ...
Li Cai-Xia CX Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Center of Molecular Medicine, Rui‑Jin Hospital, Shanghai Jiao‑Tong University School of Medicine, Shanghai 200025, P.R. - - 2014
One member of the highly conserved acidic leucine‑rich nuclear phosphoprotein 32 kDa (ANP32) family of proteins, ANP32B, is critical for normal development, as demonstrated by a study in ANP32B‑deficient mice. Another study indicated that ANP32B was a direct substrate of caspase‑3, and was primarily cleaved at the sequence Ala‑Glu‑Val‑Asp, following Asp‑163. To investigate ...
Liu Lu L Department of Preventive Dentistry, School of Stomatology, China Medical University, ShenYang, - - 2014
The present study investigated the effects of fluoride on endoplasmic reticulum (ER) stress (ERS) and osteoblast apoptosis in vivo. Forty-eight Wistar rats were randomly divided into four groups (12/group) and exposed to 0, 50, 100, and 150 mg/L of fluoride in drinking water for 8 weeks, respectively. Peripheral blood samples and bilateral ...
Cheong Taek-Chin - - 2014
Reactive oxygen species (ROS) play an important role in cellular signaling as second messengers. However, studying the role of ROS in physiological redox signaling has been hampered by technical difficulties in controlling their generation within cells. Here, we utilize two inert components, a photosensitizer and light, to finely manipulate the ...
Ji Yinghong Y Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, - - 2014
Cataract is the most common eye disease that causes blindness in patients. Ultraviolet B (UVB) irradiation is considered an important factor leading to cataract by inducing apoptosis in human lens epithelial cells (HLECs), but the mechanism is currently unclear. In this study, we investigated HLECs under different intensities of UVB ...
Wang Yu Y National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35, Tsinghua East Road, Beijing, PR - - 2014
Previous studies have shown that controlled deterioration treatment (CDT) induces programmed cell death (PCD) in elm (Ulmus pumila L.) seeds, which undergo certain fundamental processes that are comparable to apoptosis in animals. In this study, the essential characteristics of mitochondrial physiology in elm seeds during CDT were identified by cellular ...
Gram Magnus - - 2014
BackgroundIntraventricular hemorrhage (IVH) with post-hemorrhagic ventricular dilatation (PHVD) is a major cause of neurodevelopmental impairment and mortality in preterm infants. The mechanisms leading to PHVD and brain damage remain largely unknown. The choroid plexus and the ependyma, which constitute an essential part of the blood-brain barrier (BBB), are the first ...
Yoon Mi-Kyung - - 2014
p73 is a structural and functional homologue of p53 tumor suppressor protein. Like p53, p73 induces apoptosis and cell cycle arrest and transactivates p53-responsive genes, conferring its tumor suppressive activity. In addition, p73 has unique roles such as neuronal development and differentiation. The importance of p73-induced apoptosis lies in its ...
Sakata Shuhei S Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, - - 2014
Eicosapentaenoic acid (EPA) is an antioxidant and n-3 polyunsaturated fatty acid that reduces the production of inflammatory cytokines. We evaluated the role of EPA in chondrocyte apoptosis and degeneration. Normal human chondrocytes were treated with EPA and sodium nitroprusside (SNP). Expression of metalloproteinases (MMPs) was detected by real-time polymerase chain ...
Zhang Baigang B College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, - - 2014
Patulin (PAT) is one of the most widely disseminated mycotoxins found in agricultural products and could cause renal damage. However, the mechanism of cell damage remains obscure. In this study, the human embryonic kidney cells (HEK293) were treated with PAT (2.5-15 μM). The cytotoxicity was assessed with MTT assay and ...
Liu Xiaohui X Department of Environmental Health and Toxicology, School of Public Health, Dalian Medical University, Dalian 116044, - - 2014
Polybrominated diphenyl ethers (PBDEs) are a group of widely used flame retardants. The rising presence of PBDEs in human tissues has received considerable concerns with regard to potential health risks. While the mitochondrial-apoptotic pathway has been suggested in PBDEs-induced apoptosis, the role of lysosomes is yet to be understood. In ...
Kim Soo Jung SJ College of Medicine, Inha University, Sinheung-dong, Jung-gu, Incheon Republic of - - 2014
Chronic myeloid leukemia (CML) is characterized by a constitutive activation of Bcr-Abl tyrosine kinase. Bcr-Abl/T315I is the predominant mutation that causes resistance to Imatinib. In the present study, we synthesized a novel Bcr-Abl inhibitor, HS-543, and investigated its effect on cell survival or apoptosis in CML cells bearing Bcr-Abl/T315I (BaF3/T315I) ...
Szeto H H HH Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, - - 2014
Mitochondrial medicine is an evolving discipline whose importance derives from the central function of mitochondria in adenosine triphosphate (ATP) production, generation of reactive oxygen species, and cell death by necrosis or apoptosis. Consequently, mitochondrial dysfunction plays an important role in the progression of aging and the pathophysiology of many common ...
Glebov Konstantin K Department of Neurology, University of Bonn, Bonn, - - 2014
Microglia are resident immune cells in the brain and exert important functions in the regulation of inflammatory processes during infection or cellular damage. Upon activation, microglia undergo complex morphological and functional transitions, including increased motility, phagocytosis and cytokine secretion. Recent findings indicate that exosomes, small vesicles that derive from fusion ...
Ma Shan-Feng SF Central Laboratory, The First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, PR China; Department of Physiology, Bengbu Medical College, Anhui, 233030, PR - - 2014
Classically activated pro-inflammatory (M1) and alternatively activated anti-inflammatory (M2) macrophages populate the local microenvironment after spinal cord injury (SCI). The former type is neurotoxic while the latter has positive effects on neuroregeneration and is less toxic. In addition, while the M1 macrophage response is rapidly induced and sustained, M2 induction ...
Lu Jin-Ping JP Department of Internal Medicine & Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China, - - 2014
The aim of this study was to examine the effects of endoplasmic reticulum (ER) stress on aldosterone (Aldo)-induced apoptosis of endothelial cells. Glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP, a hallmark of ER-associated apoptosis) were used to evaluate ER stress. Western blotting and real-time PCR were used to ...
Shi Xin X *Department of Internal Medicine, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine (TCM), Zhengzhou, China; †Department of Cardiology, Zhengzhou Hospital of Traditional Chinese Medicine, Zhengzhou, China; and ‡Department of Functional Examination, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine (TCM), Zhengzhou, - - 2014
: Ischemia/hypoxia-induced oxidative stress is detrimental for the survival of cardiomyocytes and cardiac function. Stanniocalcin-1 (STC-1), a glycoprotein, has been found to play an inhibitory role in the production of reactive oxygen species (ROS). Here, we speculated that the overexpression of STC-1 might alleviate oxidative damage in cardiomyocytes under conditions ...
Fu Xiao-Yan XY School of Basic Medicine, Taishan Medical University, Taian, 271000, Shandong, - - 2014
Oxidative damage plays a key role in causation and progression of neurodegenerative diseases. Inhibition of oxidative stress represents one of the most effective ways in treating human neurologic diseases. Herein, we evaluated the protective effect of curcumin on PC12 cells against H2O2-induced neurotoxicity and investigated its underlying mechanism. The results ...
Kamal Ahmed A Medicinal Chemistry and Pharmacology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India. - - 2014
Podophyllotoxin, a lignan extracted from rhizomes of Podophyllum species, is a well established lead in the development of new chemical agents for the treatment of cancer. Its semi-synthetic variant, etoposide is an anticancer drug which inhibits DNA topoisomerase II causing cell cycle arrest in the S the phase. Its clinical ...
Forli Stefano S Molecular Graphics Laboratory, Dept. of Integrative Structural and Computational Biology, MB-112F The Scripps Research Institute, 10550 North Torrey Pines Road La Jolla, CA 92037-1000, USA. - - 2014
Epothilones are natural compounds isolated from a myxobacterium at the beginning of the 1990s, and showed a remarkable anti-neoplastic activity. They act through the same mechanism of action of paclitaxel, by stabilizing microtubules and inducing apoptosis. Although, their chemical structure, simpler than taxanes, makes them more suitable for derivatization. Their ...
Cui Yanmei Y State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, - - 2014
High levels of angiogenesis and resistance to apoptosis are major clinical features of hepatocellular carcinoma (HCC), a lethal disease with a high incidence worldwide. However, the precise mechanisms underlying these malignant properties remain unclear. Here, we demonstrated that acylglycerol kinase (AGK) is markedly overexpressed in HCC cell lines and clinical ...
Goswami Poonam P Toxicology Division, CSIR- Central Drug Research Institute, Lucknow, 226031, Uttar Pradesh, - - 2014
Rotenone, a pesticide, causes neurotoxicity via the mitochondrial complex-I inhibition. The present study was conducted to evaluate the role of endoplasmic reticulum (ER) stress in rotenone-induced neuronal death. Cell viability, cytotoxicity, reactive oxygen species (ROS) generation, nitrite level, mitochondrial membrane potential (MMP), and DNA damage were assessed in rotenone-treated neuro-2A ...
Yin Yuan Y The Second Hospital of Jilin University , Changchun, Jilin , - - 2014
Abstract Exposure to reactive oxygen species (ROS) leads to the development and progression of retinal degenerative diseases. However, the exact mechanisms are not fully understood. In this article, the role of angiotensin II type 1 receptor (AT1R) signaling in H2O2-induced retinal damage was examined. Mouse photoreceptor-derived 661 W cells were treated ...
Chattopadhyay Sourav S Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, - - 2014
The aim of this study was to find out the intracellular signaling transduction pathways involved in cobalt oxide nanoparticles (CoO NPs) mediated oxidative stress in vitro and in vivo system. Cobalt oxide nanoparticles released excess Co(++) ions which could activated the NADPH oxidase and helps in generating the reactive oxygen ...
Petrick Lauren L The Lipid Research Laboratory, Rappaport Faculty of Medicine and Research Institute, Technion, Haifa, 31096, Israel; The Technion Center of Excellence in Exposure Science and Environmental Health (TCEEH), Technion, Haifa, 32000, - - 2014
Nanoparticle research has focused on their toxicity in general, while increasing evidence points to additional specific adverse effects on atherosclerosis development. Arterial macrophage cholesterol and triglyceride (TG) accumulation and foam cell formation are the hallmark of early atherogenesis, leading to cardiovascular events. To investigate the in vitro atherogenic effects of ...
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