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Niles Andrew L AL Promega Corporation, 2800 Woods Hollow Road, Madison, WI, 53711, USA, - - 2015
Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice is not only more cost efficient, but also provides more information about a compound or treatment. The ability to combine the activity profiles within the same sample provides a level of normalization ...
Yu Guozheng G Department of General Surgery (GY, YB, SZ, ZL), Research Center of Digestive Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China; Department of Head and Neck Surgery (WL), Hubei Cancer Hospital, Wuhan, China; Department of Internal Medicine Oncology (LX), Huangshi Central Hospital, Huangshi, China; and Department of Orthopedic (YL), Taihe Hospital, Hubei University of Medicine, Shiyan, - - 2014
: Interleukin 34 (IL-34) is a newly recognized cytokine that functions similarly to macrophage colony-stimulating factor. This study investigated the mechanism by which IL-34 is produced in response to exogenous pathogen infections in humans. The results showed that the IL-34 levels were higher in the serum and peripheral blood mononuclear ...
Li Jia J Department of Respiratory Medicine, the Chinese PLA General Hospital, NO.28 Fuxing Road, Haidian District, 100853, Beijing, - - 2014
Circulating lymphocyte number was significantly decreased in patients with sepsis. However, it remains unknown which severity phase (sepsis, severe sepsis, and septic shock) does it develop and what happen on each subpopulation. Eight patients with differing severities of sepsis (31 sepses, 33 severe sepses, and 16 septic shocks) were enrolled. ...
Yan Qi Q The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedical Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of - - 2014
This study aimed to evaluate the effect of all-trans retinoic acid (atRA) on suppressing the inflammatory response and promoting the osteoblastic differentiation of bone marrow stromal cells (BMSCs) on titanium in a lipopolysaccharide (LPS)-induced microenvironment. BMSCs were divided into four groups and treated with LPS (1 μg/mL), atRA (1 nmol/L), LPS + atRA, or ...
Bolkun Lukasz L Department of Haematology, Medical University of Bialystok, - - 2014
Tumour necrosis factor-alfa (TNF-α) is an inflammatory cytokine with a wide spectrum of biological activity, including angiogenesis. Tumour necrosis factor-related apoptosis inducing ligand (TRAIL), which belongs to the TNF family of proteins, plays a role in the regulation of vascular responses, but its effect on the formation of new blood ...
Ma Xiaojuan X Xinjiang National Clinical Research Base of Traditional Chinese Medicine, Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China ; Department of Immunology, College of Basic Medicine, Ürümqi, Xinjiang 830011, P.R. - - 2014
The Uygur herb, Hyssopus officinalis L., has been demonstrated to affect the levels of a number of cytokines in asthmatic mice, including interleukin-4, -6 and -17 and interferon-γ. In the present study, the effect of Hyssopus officinalis L. on airway immune regulation and airway inflammation was investigated in a mouse ...
Ali Daoud D Department of Zoology, College of Science, King Saud University, P.O. Box 2454, Riyadh, 11451, Saudi Arabia, - - 2014
Silver is one of the most toxic metals to freshwater aquatic organisms. Limited efforts have been made to study apoptosis and genotoxic potential of silver nanoparticles (AgNPs) in freshwater snail Lymnea luteola L. (L. luteola). Therefore, the present investigation was aimed to study the induction of apoptosis and DNA damage ...
Pardee Angela D AD Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA - - 2014
Several tumor-derived factors have been implicated in dendritic cell (DC) dysfunction in cancer patients. α-fetoprotein (AFP) is an oncofetal Ag that is highly expressed in abnormalities of prenatal development and several epithelial cancers, including hepatocellular carcinoma (HCC). In HCC patients exhibiting high levels of serum AFP, we observed a lower ...
Yoshida Shigeo S Department of Ophthalmology, Kyushu University Graduate School of Medical Sciences, Fukuoka, - - 2014
We recently demonstrated that M2 macrophages were involved in the development of fibrovascular membranes (FVM) associated with proliferative diabetic retinopathy (PDR) possibly through the induction of periostin. The purpose of this study was to determine whether macrophage colony-stimulating factor (M-CSF) and interleukin (IL)-13, inducers of the M2 polarisation of macrophages ...
Chiewchengchol Direkrit D Center of Excellence in Immunology and Immune-mediated Disease, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, - - 2014
Juvenile systemic lupus erythematosus (JSLE) is one of the most common autoimmune diseases in children and can affect multiple organs and systems. The etiology remains unclear, and current management only suppresses rather than eliminates the disease. The pathogenesis is triggered by autoantigens that induce autoantibody production. Apoptotic neutrophils may be ...
Wang Kun K Department of Anesthesiology, The Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. - - 2014
Surgical trauma leads to a severe deterioration of the immune system. Electroacupuncture (EA) may improve the immunodeficiency that occurs following surgery; however, the underlying signaling mechanisms require further study. In the present study, 40 rats were equally randomized into four groups: Control; Control + EA; Trauma; Trauma + EA. EA was applied at the 'Zusanli' (ST36) ...
Ji Guang-Quan GQ College of Light Industry and Food Sciences, South China University of Technology , Guangzhou , China - - 2014
Abstract Context: Atractylodes macrocephala Koidz is a traditional herb. Atractylodes macrocephalaon polysaccharides (AMP) have been found to enhance immunity and improve heart function. However, the mechanisms of the immunomodulatory effect have not been investigated. Objective: We examined whether AMP activated macrophages and explored the mechanisms of activation. Materials and methods: ...
Praschberger Monika M Department of Medical Chemistry, Medical University of Vienna, Waehringerstr. 10, 1090, Vienna, - - 2014
Intravenous iron preparations, like iron sucrose (IS) and ferric carboxymaltose (FCM) differ in their physicochemical stability. Thus differences in storage and utilization can be expected and were investigated in a non-clinical study in liver parenchyma HepG2-cells and THP-1 macrophages as models for toxicological and pharmacological target cells. HepG2-cells incorporated significant ...
Baigildina Asia A AA Bashkir State Medical University, Ufa, Republic of Bashkortostan, Russian - - 2014
Aims: Nephropathia epidemica (NE) is a form of hemorrhagic fever with renal syndrome associated with the Puumala virus species of Hantavirus. The pathogenesis of NE is not well understood; therefore, investigating the inflammatory cytokine response to infection may provide useful knowledge in deciphering the pathophysiology of NE. Materials & methods: ...
Domènech A A Department of Ruminant Production, IRTA, Institute of Research in Agriculture and Technology. Torre Marimon, km 12,1 C-59, Caldes de Montbui, 08140, Barcelona, - - 2014
The dry period is a nonlactating phase in which senescent mammary cells are regenerated, which is thought to optimize milk production in the subsequent lactation. In bovines, the dry period normally coexists with pregnancy and the lactogenic hormones delay mammary gland involution and impair the activation of immune system to ...
Bhattacharya Sourav S Department of Physiology, University of Calcutta, West Bengal, - - 2014
Soup prepared from the foot of fresh water edible snail, Bellamya bengalensis, is traditionally consumed by the tribes of Jharkhand against rheumatism like bone and joint inflammation. As rheumatism have underlying involvement of cell mediated hypersensitivity, in vivo delayed-type hypersensitivity (DTH) model and in vitro LPS-induced macrophage signaling were studied ...
Engberg Anna E AE Linnæus Center of Biomaterials Chemistry, Linnæus University, SE-391 82 Kalmar, Sweden; Department of Clinical Chemistry, University and Regional Laboratories, Region Skåne, - - 2014
Inappropriate complement activation is often responsible for incompatibility reactions that occur when biomaterials are used. Complement activation is therefore a criterion included in legislation regarding biomaterials testing. However, no consensus is yet available regarding appropriate complement-activation-related test parameters. We examined protein adsorption in plasma and complement activation/cytokine release in whole ...
Coutinho Leonam G - - 2014
BackgroundBacterial meningitis (BM) is characterized by an intense host inflammatory reaction, which contributes to the development of brain damage and neuronal sequelae. Activation of the kynurenine (KYN) pathway (KP) has been reported in various neurological diseases as a consequence of inflammation. Previously, the KP was shown to be activated in ...
Czepiel J J Department of Infectious Diseases, Jagiellonian University Medical College, Cracow, Poland. jacek.czepiel@uj.edu.pl; - - 2014
It is widely accepted that the pathogenesis of Clostridium difficile infection (CDI) is multifactorial, dependent on pathogen virulence factors produced by the organism as well as disorders of the gastrointestinal tract, the alteration in intestinal flora and the immune response of the host. In particular, the immune response in the ...
Kumar Lokender L Department of Microbiology, BMS Block, Panjab University, Chandigarh 160014, - - 2014
Excessive use of antibiotics has led to evolutionary adaptation resulting in emergence of multidrug resistance in P. aeruginosa. The aim of the present study was oriented towards exploiting zingerone (active component of ginger) in making P. aeruginosa more susceptible to killing with antibiotics, humoral/cellular defences and studying its underlying mechanism. ...
Taylor Douglas D DD Exosome Sciences, Inc., 11 Deer Park Drive, Suite 103, Monmouth Junction, NJ 08852, USA - - 2014
We have previously demonstrated the release of membranous structures by cells into their extracellular environment, which are termed exosomes, microvesicles or extracellular vesicles depending on specific characteristics, including size, composition and biogenesis pathway. With activation, injury, stress, transformation or infection, cells express proteins and RNAs associated with the cellular responses ...
Kumari A A 1] Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Regents University Cancer Center, Augusta, GA 30912, USA [2] Molecular Signaling Program, Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, - - 2014
The transcription factor adenovirus E2 promoter-binding factor (E2F)-1 normally enhances cell-cycle progression, but it also induces apoptosis under certain conditions, including DNA damage and serum deprivation. Although DNA damage facilitates the phosphorylation and stabilization of E2F1 to trigger apoptosis, how serum starvation renders cells vulnerable to E2F1-induced apoptosis remains unclear. ...
Meana Clara C Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas, Universidad de Valladolid, 47003 Valladolid, Spain; and Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas, 28029 Madrid, - - 2014
Lipin-1 is a Mg(2+)-dependent phosphatidic acid phosphatase involved in the de novo synthesis of phospholipids and triglycerides. Using macrophages from lipin-1-deficient animals and human macrophages deficient in the enzyme, we show in this work that this phosphatase acts as a proinflammatory mediator during TLR signaling and during the development of ...
Kung Chia-Te CT Department of Emergency Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, - - 2014
Cell apoptosis in critically-ill patients plays a pivotal role in the pathogenesis of sepsis. This study aimed to determine the prognostic value of leukocyte apoptosis in patients with severe sepsis. Leukocyte apoptosis was determined by flow cytometry. The values of annexin V, APO2.7, and 7-amino-actinomycin D (7AAD) for each subtype ...
Hel Zdenek Z Department of Pathology and Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, - - 2014
Common variable immunodeficiency (CVID) is the most frequent form of primary symptomatic hypogammaglobulinemia. CVID patients display a number of abnormalities in lymphocyte subpopulations including chronic T-cell activation and decreased numbers of circulating CD4(+) T cells and NK cells. We and others have recently shown that CVID is associated with increased ...
Wang P W PW Department of Pediatrics, Taipei City Hospital, Women and Children's Campus - - - 2014
We investigated the effect of short--term pure oxygen treatment on pulmonary function, serum cytokine levels, and apoptosis in the lungs of healthy newborn piglets. Twelve newborn piglets were randomly assigned to receive pure oxygen (OXY) (n=6) or room air (AIR: 21% oxygen) (n=6) for 4 hours. Cardiopulmonary function serum levels ...
Wang Fang F State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, - - 2014
Golgi protein 73-kD (GP73), a type II Golgi transmembrane glycoprotein, is highly expressed in a variety of human diseases, but its physiology and pathology remain unknown. In examining the function of GP73 in the immune system, treatment of human PBMC with ConA significantly increased the intracellular expression of GP73 and ...
Yang Si-Dong SD Department of Spinal Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, - - 2014
Abstract Levofloxacin, a fluoroquinolone, is a widely-used and effective antibiotic. However, various adverse side effects are associated with levofloxacin. The purpose of this study was to further explore the effects of levofloxacin on rat nucleus pulposus cells. Inverted phase-contrast microscopy, flow cytometry, and caspase-3 activity assays were used, and revealed ...
Zhang Pei P Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China. Electronic address: - - 2014
The in vivo and in vitro immunostimulating properties of chitosan oligosaccharide (COS) prepared by enzymatic hydrolysis of chitosan and the mechanisms mediating the effects were investigated. Our data showed that the highly active chitosanase isolated could hydrolyze chitosan to the polymerization degree of 3-8. The resulting COS was an efficient ...
Díaz-Zúñiga Jaime J Periodontal Biology Laboratory, Department of Conservative Dentistry, Dental School, Universidad de Chile, Santiago de Chile, - - 2014
Background: Different serotypes of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis have shown to induce differential dendritic cell (DC) response. This study aimed to investigate whether the cytokine and CC-chemokine receptor (CCR) production by DCs stimulated with the different serotypes of A. actinomycetemcomitans or P. gingivalis was toll-like receptor (TLR)2 and/or TLR4-dependent. ...
Matsushita A A Department of Food and Nutrition Science, Sagami Women's Junior College, Sagamihara, - - 2014
Repeated exposure to allergens induces chronic allergic lesions in the skin and a shift in the cutaneous cytokine milieu to T helper (Th)2. To assess the relationships between Th17 and Th2 response during allergic contact dermatitis (ACD) in mice. ACD was induced in C57BL/6 mice by single or repeated epicutaneous ...
Leong Ka Wai KW Department of Biological Sciences, National University of Singapore , Singapore , - - 2014
The roles of human serum IgA, in contrast to that of mucosal IgA, are relatively unexplored. Previous studies have shown that IgA mediates either pro- or anti-inflammatory effects in innate immune cells. Serum IgA has been shown to interact with many proteins and glycoproteins of which the functions and mechanisms ...
Hand Laura E LE Faculty of Life Sciences, University of Manchester, Manchester, - - 2014
Obesity is a major risk factor for metabolic disease, with white adipose tissue (WAT) inflammation emerging as a key underlying pathology. We detail that mice lacking Reverbα exhibit enhanced fat storage without the predicted increased WAT inflammation or loss of insulin sensitivity. In contrast to most animal models of obesity ...
Fogarty Christopher L CL Folkhälsan Research Center, Biomedicum Helsinki (Room C317b), Haartmaninkatu 8, 00290, Helsinki, - - 2014
Dietary fats have been shown to promote the translocation of bacterial endotoxins from the gut into circulation, which may induce systemic inflammation and modulate the inflammatory response of circulating immune cells. The aim of this study was to determine the effect of the postprandial milieu on inflammation and the inflammatory ...
Solberg Rigmor - - 2014
Abstract Macrophages express several lysosomal cysteine proteases such as cathepsins and legumain. In this study, we assessed the expression, activity and secretion of legumain in cellular models of monocytes/macrophages. Macrophages were derived from M-CSF- or GM-CSF/IFNγ-stimulated human primary monocytes (M2 and M1, respectively), PMA-treated human THP-1 cells, or murine RAW264.7 ...
Ma Liangliang - - 2014
Previous studies have indicated that autoimmune diseases might be caused by an imbalance of T helper cells (Th), cytokines, and regulatory T cells (Treg) cytokines. We measured the plasma concentrations of Th1-associated cytokines (IFN-gamma, IL-2), Th2 -associated cytokines (IL-4, IL-10), Th17-associated cytokine (IL-17) and Treg -associated cytokine (TGF-beta1) in adult ...
Hostanska Katarina K Institute for Complementary Medicine, University Hospital Zurich, Zurich, - - 2014
OBJECTIVES : Extracts of the tubers of Harpagophytum procumbens (devil's claw, DC) inhibit different proinflammatory mediators important in the pathophysiology of osteoarthritis. Many plant-derived preparations interfere with cytochrome P450 liver enzymes, which influence their different biological activities. Therefore, the present study was designed to investigate the influence of an external metabolic ...
Zhang Hangjun H Department of Environmental Sciences, Hangzhou Normal University, Xuelin Road 16#, Hangzhou, Zhejiang Province 310036, PR China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou, Zhejiang Province 310036, PR China. Electronic address: - - 2014
Perfluorooctanoic acid (PFOA), a prominent perfluorinated compound (PFC), has been widely detected in natural water bodies worldwide. In this study, zebrafish (Danio rerio) was exposed to nominal concentrations of PFOA (0.05, 0.1, 0.5, and 1 mg/L) for 21 d. After exposure, each fish was decapitated, and the spleen was removed to detect ...
Stoffels Monique M Department of Internal Medicine, Radboud University Medical Center and Nijmegen Institute for Infection, Inflammation and Immunity (N4i), Nijmegen, The Netherlands. Department of Internal Medicine, Radboud University Medical Center and Nijmegen Institute for Infection, Inflammation and Immunity (N4i), Nijmegen, The - - 2014
The autoinflammatory hyperimmunoglobulinaemia D and periodic fever syndrome (HIDS) is characterized by recurrent episodes of fever and inflammation. As part of the mevalonate kinase deficiency spectrum, it is caused by MVK mutations, resulting in decreased mevalonate kinase activity in the isoprenoid pathway. Although IL-1β is considered a major cytokine in ...
Stewart Adam Michael AM ZENEREI Institute, 309 Palmer Court, Slidell 70458, USA; Neuroscience Program, Tulane University Medical School, 1430 Tulane Avenue, New Orleans, LA 70112, - - 2014
Mounting clinical and experimental evidence implicates various cytokines in stress-related affective brain disorders. Here, we analyze behavioral phenotypes in C57BL/6J male mice following the chronic social defeat stress paradigm, and examine their serum cytokines and corticosterone levels. Loser mice experiencing 20 days of daily 15-min social confrontations demonstrate elevated levels ...
Hollenbaugh Joseph A - - 2014
BackgroundSAMHD1 degrades deoxyribonucleotides (dNTPs), suppressing viral DNA synthesis in macrophages. Recently, viral protein X (Vpx) of HIV-2/SIVsm was shown to target SAMHD1 for proteosomal degradation and led to elevation of dNTP levels, which in turn accelerated proviral DNA synthesis of lentiviruses in macrophages.ResultsWe investigated both time-dependent and quantitative interplays between ...
Li Defang D Department of Orthopedic Surgery, Jinshan Hospital, Fudan University, Shanghai, 201508 China. Electronic address: - - 2014
The apoptosis of cartilage endplates (CEPs), acting as an initiating factor, plays a vital role in the pathogenesis of intervertebral disc degenerative diseases, the underlying molecular mechanism of the apoptotic process in CEPs is still not clear. The present study aimed to investigate the mechanism of CEP cell apoptosis. We ...
Antonelli Alessandro A Department of Clinical and Experimental Medicine, University of Pisa, Via Savi 10, 56126, Pisa, Italy. Electronic address: - - 2014
To our knowledge, no study has evaluated the involvement of T helper (Th)1- and Th2-chemokines in extra-ocular muscle (EOM) myopathy in "patients with thyroid-associated ophthalmopathy" (TAO-p). We tested the effects of interferon (IFN)γ and tumor necrosis factor (TNF)α stimulation, and of increasing concentrations of peroxisome proliferator-activated receptor (PPAR)γ agonists (pioglitazone ...
Medrano-Engay B B Translational Research Unit, Miguel Servet University Hospital, P Isabel la Católica 1-3, 50009 Zaragoza, Spain; Instituto Aragonés de Ciencias de la Salud (IACS), Avda. San Juan Bosco, 13, 50009 Zaragoza, - - 2014
Gaucher disease induces some metabolic abnormalities so increased serum ferritin appears in more than 60% at diagnosis. The storage of glucosylceramide in macrophages produces an inflammatory response with iron recycling deregulation and release of cytokines. Iron homeostasis is controlled by the circulating peptide hepcidin and its production is influenced by ...
Zhang Xin X Pain Management Center, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, - - 2014
Background/Aims: Microglia, which represent the immune cells of the central nervous system (CNS), have long been a subject of study in CNS disease research. Substantial evidence indicates that microglial activation functions as a strong neuro-inflammatory response in neuropathic pain, promoting the release of pro-inflammatory cytokines, such as tumor necrosis factor ...
Sesso Maria Lucia Talarico ML Department of Pediatrics, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: - - 2014
Little is known about the ontogeny of the cytokines in saliva of newborn. Previous studies showed that levels of immunoglobulin A (IgA) in saliva could be influenced by prematurity. So, the aim of this study was to analyze the levels of interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12), ...
Ikeda Naho N Neonatal Center, Juntendo University Shizuoka Hospital, Shizuoka, - - 2014
Enterococcus faecalis is rarely involved in neonatal meningitis. Several studies have indicated that the cytokines related to bacterial infection may induce nerve cell damage; therefore, the cytokine levels in cerebrospinal fluid (CSF) could represent a valuable hallmark for rapid recognition of the disease and evaluation of the degree of neurological ...
Osikov M V MV Department of Pathological Physiology, Chelyabinsk State Medical Academy, Chelyabinsk, Russia, - - 2014
The effect of recombinant human epidermal growth factor (rhEGF) on innate immunity and cellular composition of the destruction focus in the third-degree (IIIA) burn (skin contact with an object heated to 100°C; 4% body surface) was studied in experiments on outbred albino rats. On days 7-28 after burn, blood count ...
Mencl Stine S Department of Neurology, University Hospital Würzburg, Würzburg, - - 2014
Inflammation is a pathological hallmark of traumatic brain injury (TBI). Recent evidence suggests that immune cells such as lymphocytes are of particular relevance for lesion development after TBI. FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, sequesters T lymphocytes in lymphoid organs and has been shown to improve outcome in a variety ...
Zhao Songfeng S Department of Pharmacology, the Provincial Key Lab of Clinical Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, - - 2014
Epidermal burn injury may trigger significant apoptosis of the spleen cells, which might be caused by a burn-induced systemic inflammatory reaction. Heparin has been shown to possess anti-inflammatory properties. Interleukin 1 (IL-1) is centrally important among pro-inflammatory cytokines. We hypothesized that heparin might inhibit burn-induced apoptosis in the spleen via ...
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