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Hsu Daniel K DK Department of Dermatology, University of California, Davis, Davis, - - 2015
Galectins are a family of animal lectins with conserved carbohydrate-recognition domains that recognize β-galactosides. Despite structural similarities, these proteins have diverse functions in a variety of cellular processes. While a large number of extracellular functions have been demonstrated for galectins, the existence of intracellular functions has been clearly shown for ...
Woodham Andrew W AW Department of Molecular Microbiology and Immunology, Keck School of Medicine/Norris Comprehensive Cancer Center, University of Southern California, ZNI Room 245, 1501 San Pablo Street, Los Angeles, CA, 90089, - - 2015
Langerhans cells (LC) are the resident antigen-presenting cells in human epithelium, and are therefore responsible for initiating immune responses against human papillomaviruses (HPV) entering the epithelial and mucosal layers in vivo. Upon proper pathogenic stimulation, LC become activated causing an internal signaling cascade that results in the up-regulation of co-stimulatory ...
Westerberg Christine Möller CM Clinical Immunology and Allergy Unit, Department of Medicine, Karolinska University Hospital Solna L2:04, Stockholm, 171 76, - - 2015
One key characteristic of certain mast cell populations is their longevity. Mast cell survival can also be promoted by Fc-receptor activation. Regulation of cell survival and apoptosis is regulated by the Bcl-2 family that consists of pro- and anti-apoptotic proteins. Depending on their relative cellular expression levels, the cells are ...
Dingding An A 1Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, 2Harvard Medical School, Boston, Massachusetts, 3Harvard Medical School, Boston, - - 2014
The role microbes play in shaping host immune system and intestinal health has been increasingly appreciated. However, the majority of the evidences are from epidemiological studies with only limited insights on molecular mechanisms. We have previously uncovered that symbiotic microbes regulate the host invariant natural killer T (iNKT) cell homeostasis ...
Fowler Benjamin J BJ Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, KY 40536, USA. Department of Physiology, University of Kentucky, Lexington, KY 40536, - - 2014
Nucleoside reverse transcriptase inhibitors (NRTIs) are mainstay therapeutics for HIV that block retrovirus replication. Alu (an endogenous retroelement that also requires reverse transcriptase for its life cycle)-derived RNAs activate P2X7 and the NLRP3 inflammasome to cause cell death of the retinal pigment epithelium in geographic atrophy, a type of age-related ...
Fossum Even E K.G.Jebsen Center for Influenza Vaccine Research, Institute of Immunology, University of Oslo and Oslo University Hospital, Oslo, - - 2014
Targeting antigens to cross-presenting dendritic cells (DCs) is a promising method for enhancing CD8(+) T-cell responses. However, expression patterns of surface receptors often vary between species, making it difficult to relate observations in mice to other animals. Recent studies have indicated that the chemokine receptor Xcr1 is selectively expressed on ...
Geissler Klaus K 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria; Ludwig Boltzmann Institute for Clinical Oncology, Vienna, - - 2014
The spontaneous formation of colony-forming units granulocyte/macrophage (CFU-GM) in semisolid cultures has been shown to be due to the endogenous release of cytokines and/or to the hypersensitivity of cells against growth factors. We have reported that increased autonomous CFU-GM growth is an in vitro characteristic of myelofibrosis (MF) which may ...
Alidadiyani Neda N Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , - - 2014
In this study, the ability of methotrexate (MTX)-loaded stimuli-responsive novel silica nanocomposites (MSNs) (with mean diameter of ± 60 nm) in the induction of apoptosis, and change in the Bax/Bcl-2 mRNA levels, were investigated. MTT assay and RT -PCR analysis were performed on MDA-MB-231 breast cancer cells, to evaluate their ...
Jiang C M CM State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chendu, Sichuan, - - 2014
The environment of bone marrow mesenchymal stem cells (MSCs) is hypoxic, which plays an important role in maintaining their self-renewal potential and undifferentiated state. MSCs have been proven to possess immunomodulatory properties and have been used clinically to treat autoimmune diseases. Here, we tested the effects of hypoxia on the ...
Yu Pengfei P School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning, 110016, - - 2014
The purposes of this study are to investigate the antitumor activities of NSK-01105, a novel sorafenib derivative, in in vitro and in vivo models, and explore the potential mechanisms. The effects of NSK-01105 on proliferation and apoptosis of prostate cancer cells were established by cytotoxicity assays, apoptosis analysis, flow cytometry ...
Wynne James W - - 2014
BackgroundBats are a major reservoir of emerging infectious viruses. Many of these viruses are highly pathogenic to humans however bats remain asymptomatic. The mechanism by which bats control viral replication is unknown. Here we utilize an integrated approach of proteomics informed by transcriptomics to compare the response of immortalized bat ...
Jin Di D Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, - - 2014
Catalpol is an iridoid glucoside extracted from the traditional Chinese medicinal plant called Rehmannia glutinosa, and it is reported to possess a variety of pharmacological activities. The goal of this research was to explore the effect of catalpol on the human bladder cancer cells T24. The cells were treated for ...
Dai Xiao-Jing XJ Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, Jiangsu, 221002, - - 2014
Microglia play an important role in neuronal protection and damage. However, the molecular and cellular relationship between microglia and neurons is unclear. We carried out a prospective study to detect that activation of BV2 microglia induced PC12 cell apoptosis in vitro through the TLR4/adapter protein myeloid differentiation factor 88 (MyD88)/nuclear ...
Nakagawa Akihisa A Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado, - - 2014
Inactivation of cell-survival factors is a crucial step in apoptosis. The phosphoinositide 3-kinase (PI3K)-AKT signaling pathway promotes cell growth, proliferation and survival, and its deregulation causes cancer. How this pathway is suppressed to promote apoptosis is poorly understood. Here we report the identification of a CED-3 caspase substrate in Caenorhabditis ...
Ma Weiwei W School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing, People's Republic of China - - 2014
Abstract Genistein (GEN), a major soybean isoflavone (SIF), might possess neuroprotective properties through its anti-inflammatory activity. We hypothesized that GEN could prevent the inflammatory damage detected in C6 cells induced by β-amyloid peptides 25-35 (Aβ25-35). Accordingly, we evaluated the inflammatory damage induced by Aβ25-35 and the protective effect of GEN ...
Ihlefeld Katja K Klinikum der Goethe-Universitaet, - - 2014
Sphingosine 1-phosphate (S1P) is an extra- and intracellular mediator that regulates cell growth, survival, migration and adhesion in many cell types. S1P lyase is the enzyme which irreversibly cleaves S1P and thereby constitutes the ultimate step in sphingolipid catabolism. It has been reported previously that embryonic fibroblasts from S1P lyase-deficient ...
Schinnerling Katina K Medizinische Klinik I, Charité-Universitätsmedizin Berlin, CBF, Berlin, - - 2014
Accumulation of Tropheryma (T.) whipplei-stuffed macrophages in the duodenum, impaired T. whipplei-specific Th1 responses and weak secretion of IL-12 are hallmarks of classical Whipple's disease (CWD). This study addresses dendritic cell (DC) functionality during CWD. We documented composition, distribution and functionality of DC ex vivo or after in vitro maturation ...
Yuan Jing J Department of Hematology, Peking University First Hospital, 8 Xishiku Street, Xicheng District, 100034, Beijing, - - 2014
Blockade of CC chemokine receptor 5 (CCR5) by maraviroc may induce immunological changes independent of its antiviral effects and may have immunoregulation properties. This study was designed to determine the effects of blocking CCR5 on human activated T cells in vitro and investigate the potential immunological mechanisms. Human CD3+ T ...
Lee Sue Hyun SH Department of Biomedical Engineering, Vanderbilt University, Nashville TN 37235, USA. Tel: - - 2014
A reactive oxygen species (ROS)-degradable scaffold is fabricated by crosslinking biocompatible, hydrolytically-degradable poly(ε-caprolactone) (PCL) with a ROS-degradable oligoproline peptide, KP7K. The ROS-mediated degradability triggers favorable host responses of the scaffold including improved cell infiltration and angiogenesis in vivo, indicating its unique advantages for tissue engineering applications.
Sauter Alexander A Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5021, Bergen, - - 2014
Ex vivo generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell-culture dishes. In order to elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with 4 different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 ...
Liu Bin B Laboratory of Hepatobiliary Surgery, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. - - 2014
Dihydromyricetin (DHM) is a flavonoid compound which possesses potent antitumor activity. In the present study, it was demonstrated that DHM significantly inhibited proliferation and induced apoptosis in mouse hepatocellular carcinoma Hepal‑6 cells. Transforming growth factor β (TGF‑β) is recognized as a major profibrogenic cytokine and is therefore a common target ...
Chao Pin-Zhir PZ Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Department of Otolaryngology, Shuang-Ho Hospital, New Taipei, - - 2014
Dendritic cells (DCs) play an essential role in immunity and are used in cancer immunotherapy. However, these cells can be tuned by tumors with immunosuppressive responses. DC-specific intercellular adhesion molecule 3-Grabbing Nonintegrin (DC-SIGN), a C-type lectin expressed on DCs, recognizes certain carbohydrate structures which can be found on cancer cells. ...
Xu Gong-Ping GP Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, 148 Baojian Road, Harbin, 150081, - - 2014
Matrine, a natural product, has been demonstrated to be a promising chemotherapeutic drug for some cancers. Using flow cytometric analysis of the cell cycle and apoptosis, we found that matrine inhibited the proliferation and induced apoptosis in the human osteosarcoma (OS) cell lines MG63, HOS, U2OS, and SAOS2 in vitro ...
Karumuthil-Melethil Subha S Department of Surgery, University of Illinois at Chicago, Chicago, - - 2014
The progression of autoimmune diseases is dictated by deviations in the fine balance between pro-inflammatory versus regulatory responses and pathogen recognition receptors (PRRs) play a key role in maintaining the balance. Previously, we have reported that ligation of TLR2 and Dectin 1 on APCs by zymosan results in a regulatory ...
Audrito Valentina V Department of Medical Sciences, University of Torino, - - 2014
Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in NAD biosynthesis. In the extracellular compartment it exhibits cytokine/adipokine-like properties, suggesting that it stands at the crossroad between metabolism and inflammation. Here we show that both intracellular and extracellular NAMPT levels are increased cells and plasma of chronic lymphocytic leukemia (CLL) patients. ...
El-Mesery Mohamed M Division of Molecular Internal Medicine, Department of Internal Medicine II, University Hospital Würzburg; Department of Biochemistry, Faculty of Pharmacy, Mansoura University, - - 2014
MLN4924 prevents the formation of active cullin-RING ubiquitin ligase complexes and thus inhibits NFκB signaling. Here, we evaluated the effects of this compound on monocytes and dendritic cells (DCs). Monocytes and DCs were challenged with TNF or LPS in the presence and absence of MLN4924. The effects of MLN4924 on ...
Dutta Partha P Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, - - 2014
Monocytes are part of the vertebrate innate immune system. Blood monocytes are produced by bone marrow and splenic progenitors that derive from hematopoietic stem cells (HSCs). In cardiovascular disease, such as atherosclerosis and myocardial infarction, HSCs proliferate at higher levels that in turn increase production of hematopoietic cells, including monocytes. ...
Wang John J Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University Langone Medical Center, New York, NY 10016, - - 2014
Chemokines are a group of small, secreted molecules that signal through G protein-coupled receptors to promote cell survival and proliferation and to provide directional guidance to migrating cells. CXCL12 is one of the most evolutionary conserved chemokines and signals through the chemokine receptor CXCR4 to guide cell migration during embryogenesis, ...
Gao Xue Juan XJ Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, Guangdong, - - 2014
The protozoan Toxoplasma gondii expresses large amounts of a 37 kDa Type 2C serine-threonine phosphatase, the so-called TgPP2C which has been suggested to contribute to parasite growth regulation. Ectopic expression in mammalian cells also indicated that the enzyme could regulate growth and survival. In this study, we aimed to investigate ...
Gianni Tatiana T Department of Experimental, Diagnostic and Specialty Medicine, Alma Mater Studiorum-University of Bologna, Bologna, - - 2014
TLR2 is a cell surface receptor which elicits an immediate response to a wide repertoire of bacteria and viruses. Its response is usually thought to be proinflammatory rather than an antiviral. In monocytic cells TLR2 cooperates with coreceptors, e.g. CD14, CD36 and αMβ2-integrin. In an earlier work we showed that ...
Escudero-Pérez Beatriz B Molecular Basis of Viral Pathogenicity, CIRI, INSERM U1111- CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon, - - 2014
During Ebola virus (EBOV) infection a significant amount of surface glycoprotein GP is shed from infected cells in a soluble form due to cleavage by cellular metalloprotease TACE. Shed GP and non-structural secreted glycoprotein sGP, both expressed from the same GP gene, have been detected in the blood of human ...
Suzuki-Yamamoto Toshiko T Department of Nutritional Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, - - 2014
Prostaglandin E2 plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. Prostaglandin E2 is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin E synthase. In some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin E synthase-1 are transiently induced, leading to prostaglandin ...
Tripathi Sudipta - - 2014
Tolerance of the fetus by the maternal immune system is regulated through various mechanisms involving the different immune cells, both in the periphery and locally at the feto-maternal interface. The maternal T lymphocytes are aware of the paternal fetal antigens and a state of dynamic T cell homeostasis is maintained ...
Epanchintsev Alexey A Institute of Genetics and Molecular and Cellular Biology, Department of Functional Genomics and Cancer Biology, Centre National de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale Illkirch Cedex, - - 2014
Fanconi anemia (FA) is a rare autosomal recessive genetic disorder associated with a bone-marrow failure, genome instability, hypersensitivity to DNA crosslinking agents and a predisposition to cancer. Mutations have been documented in 16 FA genes that participate in the FA-BRCA DNA repair pathway, a fundamental pathway in the development of ...
Schögler Aline A Division of Paediatric Respiratory Medicine, University Children's Hospital, Berne, Switzerland Dept of Clinical Research, University of Berne, Berne, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Berne, Berne, - - 2014
Virus-associated pulmonary exacerbations, often associated with rhinoviruses (RVs), contribute to cystic fibrosis (CF) morbidity. Currently, there are only a few therapeutic options to treat virus-induced CF pulmonary exacerbations. The macrolide antibiotic azithromycin has antiviral properties in human bronchial epithelial cells. We investigated the potential of azithromycin to induce antiviral mechanisms ...
Hao Hua H Department of Pathology, The Second Affiliated Hospital of Nanchang University, No. 1 MinDe Road, Jiangxi Province, Nanchang City, 330006, China, - - 2014
Uterine cervical carcinoma (UCC) is one of the most common malignant tumors in females, and UCC has a close relationship with chronic cervicitis. As the endogenous "braking signal," lipoxins can regulate anti-inflammation and the resolution of inflammation. We investigated the effect of lipoxin A4 (LXA4) on the proliferation, apoptosis, and ...
Park Sang Yoon SY NICHD/NIH, United - - 2014
Vpu is an accessory protein encoded by HIV-1 that interferes with multiple host-cell functions. Herein we report that expression of Vpu by transfection into 293T cells causes partial proteolytic cleavage of interferon regulatory factor 3 (IRF3), a key transcription factor in the innate anti-viral response. Vpu-induced IRF3 cleavage is mediated ...
Ansary Md Mesbah Uddin MM *Department of Internal Medicine II, School of Medicine, Shimane University, Izumo, Japan; †Department of Gastrointestinal Endoscopy, Shimane University Hospital, Izumo, Japan; and ‡Research Division, JIMRO Co. Ltd., Takasaki, - - 2014
: Apoptosis is a programmed physiological death of unwanted cells, and handling of apoptotic cells (ACs) is thought to have profound effects on immune-mediated disorders. However, there is scant information regarding the role of ACs in intestinal inflammation, in which immune homeostasis is a major concern. To investigate this, we ...
Ni Pan - - 2014
Cisplatin is one of the most commonly used in the treatment of gastric cancer. However, drug resistance is a major obstacle for effective treatment and originates in multiple mechanisms such as enhanced DNA repair and anti-apoptosis. Our previous results demonstrated that XRCC1 was a key regulator of cisplatin induced DNA ...
Jung Joo-Yong JY Department of Biology, Briar Cliff - - 2014
Dendritic cells (DCs) are potent antigen presentation cells necessary to establish effective adaptive immune responses. The cytokine environment that exists at the time of DC differentiation may be an important but often ignored determinant in the phenotypic and functional properties of DCs. Interleukin (IL)-27 is a unique cytokine that has ...
Lakshmikuttyamma A - - 2014
siRNA is currently the most widely studied form of RNAi, and has a promising therapeutic potential in not just cancer but in other diseases such as autoimmune and infectious diseases. However, efficient delivery of siRNA to target cells is being limited by lack of an effective delivery system that ensures ...
Petri Marcelo H MH Experimental Cardiovascular Research - - 2014
The formyl peptide receptor (FPR) subtype FPR2/ALX transduces pro-inflammatory responses and participates in the resolution of inflammation depending on activation. The aim of the present study was to unravel the role of FPR2/ALX signaling in atherosclerosis. Expression of FPR2/ALX was analyzed in 127 human carotid atherosclerotic lesions and revealed that ...
Mori Masaki M Department of Orthopaedic Surgery, Kagawa University School of Medicine, Miki‑cho, Kagawa 761‑0793, - - 2014
Heat shock protein 90 (Hsp90) is constitutively expressed at 2‑10‑fold higher levels in tumor cells compared to normal cells, suggesting that it may be critically important for tumor cell growth and survival. These features make Hsp90 a potential target for anticancer drug development. Inhibition of Hsp90 activity not only results in rapid ...
Zhou Yan Y Life Sciences Institute and Innovation Center for Cell Biology, Zhejiang University, Hangzhou, Zhejiang 310058, - - 2014
Ubiquitination is generally considered as a eukaryotic protein modification, which is catalyzed by a three-enzyme cascade and is reversed by deubiquitinating enzymes. Ubiquitination directs protein degradation and regulates cell signaling, thereby plays key roles in many cellular processes including immune response, vesicle trafficking and cell cycle. Bacterial pathogens inject a ...
Xu Hao H Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee, WI, United - - 2014
High Mobility Group Box 1 (HMGB1) is a chromatin binding protein that maintains DNA structure. Upon cellular activation or injury, HMGB1 is released from activated immune cells or necrotic tissues and acts as a Damage-Associated Molecular Pattern (DAMP) to activate Toll-Like Receptor 4 (TLR4). Very little is known concerning HMGB1 ...
Luan Zhou Z Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. - - 2014
The enhancement of apoptosis is a therapeutic strategy used in the treatment of cancer. Tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) is a promising antitumor agent. However, hepatocellular carcinoma (HCC) cells exhibit marked resistance to the induction of cell death by TRAIL. The present study investigated whether rocaglamide, a naturally occurring ...
Rosser Elizabeth C EC Centre for Rheumatology, Division of Medicine, University College London, London, - - 2014
Regulatory B cells (Breg cells) differentiate in response to inflammation and subsequently restrain excessive immune responses via the release of interleukin-10 (IL-10). However, the precise inflammatory signals governing their differentiation remain to be elucidated. Here we show that the gut microbiota promotes the differentiation of Breg cells in the spleen ...
Kim Dae Sung DS Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, - - 2014
Plants use a variety of innate immune regulators to trigger cell death and defense responses against pathogen attack. We identified pepper (Capsicum annuum) GLYCINE-RICH RNA-BINDING PROTEIN1 (CaGRP1) as a RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 (CaPIK1)-interacting partner, based on bimolecular fluorescence complementation and coimmunoprecipitation analyses as well as gene silencing and transient ...
Xu Ye Y Medical Research Laboratory, Jilin Medical College, Changchun, Jilin 130021, P.R. - - 2014
Cisplatin is commonly used as a therapeutic agent, despite its known adverse side effects and the occurrence of drug resistance. The development of novel methods for combination therapy with cisplatin is required in order to circumvent these limitations of cisplatin alone. The proteasome inhibitor lactacystin (LAC) has been indicated to ...
Hu Ruozhen R Department of Pathology, Division of Microbiology and Immunology, University of Utah, 4280 JMRB, 15 North Medical Dr. East, Salt Lake City, UT 84112, - - 2014
Chronic inflammation is a contributing factor to most life-shortening human diseases. However, the molecular and cellular mechanisms that sustain chronic inflammatory responses remain poorly understood, making it difficult to treat this deleterious condition. Using a mouse model of age-dependent inflammation that results from a deficiency in miR-146a, we demonstrate that ...
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