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Taverna Francesca F Immunohematology and Transfusion Medicine Service, Fondazione IRCCS, Istituto Nazionale dei Tumori, Milan, - - 2014
Extracorporeal photochemotherapy (ECP) is a treatment approved by the FDA for cutaneous T-cell lymphoma, and it is currently used off-label for graft-versus-host disease (GvHD) and other conditions. In agreement with good practices for the therapeutic use of human cells, quality control has to be performed to validate the ECP procedure ...
Hsu Shu-Fen SF Department of Nursing, Shu-Zen Junior College of Medicine and Management, Kaohsiung, - - 2014
Cathepsin B is one of the major lysosomal cysteine proteases that plays an important role in apoptosis. Herein, we investigated whether Cathepsin B is involved in cardiomyocyte apoptosis caused by hyperthermic injury (HI) and heat shock protein (HSP)-70 protects these cells from HI-induced apoptosis mediated by Cathepsin. HI was produced ...
Won Shen-Jeu SJ Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, - - 2014
Our previous reports showed that justicidin A (JA), a novel and pure arylnaphthalide lignan isolated from Justicia procumbens, induces apoptosis of human colorectal cancer cells and hepatocellular carcinoma cells, leading to the suppression of both tumor cell growth in NOD-SCID mice. Here, we reveal that JA induces autophagy in human ...
Shin Ji-Ae JA Department of Oral Pathology, School of Dentistry, Institute of Biodegradable Material, Institute of Oral Bioscience, Chonbuk National University, Jeonju, - - 2014
The apoptotic activity of methanol extracts of Impatiens balsamina L. (MEIB) and related mechanisms in human oral squamous cell carcinoma (OSCC) cells have been systematically investigated. The effects of MEIB on human OSCC cell lines were investigated using trypan blue exclusion assay, MTS assay, Western blot, 4'-6-diamidino-2-phenylindole (DAPI) staining, Live/Dead ...
Kim Sung-Whan SW Institute for Bio-Medical Convergence, College of medicine, Catholic Kwandong University, Gangneung, Korea; Innovative Cell & Gene Therapy Center, International St. Mary's Hospital, Incheon, - - 2014
Flt3 ligand (FL), a potent hematopoietic cytokine, plays an important role in development and activation of dendritic cells (DCs) and natural killer cells (NK). Although some post-receptor signaling events of FL have been characterized, the role of FL on Flt3 expressing human peripheral blood monocyte is unclear. In the current ...
Koenigs Volker V Institut fuer Molekulare Zellbiologie, - - 2014
RhoA is thought to be essential for coordination of the membrane protrusions and retractions required for immune cell motility and directed migration. Whether the subfamily of Rho (Ras homolog) GTPases (RhoA, RhoB and RhoC) is actually required for the directed migration of primary cells is difficult to predict. Macrophages isolated ...
Obakan Pinar P Department of Molecular Biology and Genetics, Istanbul Kultur University, Atakoy Campus, Bakirkoy, Istanbul, - - 2014
Epibrassinolide (EBR), is a member of the brassinosteroids (BR), has been shown as an apoptotic inducer in different cancer cell lines. We previously showed that EBR induced apoptosis by activating polyamine catabolic pathway, which lead to the accumulation of cytotoxic compounds such as hydrogen peroxide and aldehydes in LNCaP and ...
Wolfmeier Heidi H Department of Cell Biology, Institute of Anatomy, University of Bern, Baltzerstrasse 2, 3000 Bern 9, - - 2014
Pneumolysin (PLY), a key virulence factor of S. pneumonia, permeabilizes eukaryotic cells by forming large trans-membrane pores. PLY imposes a puzzling multitude of diverse, often mutually excluding actions on eukaryotic cells. Whereas cytotoxicity of PLY can be directly attributed to the pore-mediated effects, mechanisms that are responsible for the PLY-induced ...
Rosenblat Mira - - 2014
Abstract In our in vitro study we analyzed the effects of incubation of J774A.1 macrophages with reduced glutathione (GSH) and quercetin on the extent of cellular cholesterol efflux by HDL or by apoA1. This combination was the most potent one among other exogenous and endogenous antioxidant combinations, since it significantly ...
Wachter Andreas A Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany. Electronic address: - - 2014
Initially described as an RNA surveillance pathway, nonsense-mediated decay (NMD) is also recognized to function in the regulation of host gene expression. In this issue of Cell Host & Microbe, three studies describe NMD-mediated defense strategies of plants and mammalian cells in response to pathogen infection.
Bellezza Ilaria I Experimental Medicine Department, Polo Unico S. Andrea delle Fratte, University of Perugia, 06124 Perugia, - - 2014
Cyclic dipeptides (CDPs) are a group of hormone-like molecules that are evolutionarily conserved from bacteria to humans. In bacteria, CDPs are used in quorum sensing (QS) to communicate information about population size and to regulate a behavioural switch from symbiosis with their host to virulence. In mammals, CDPs have been ...
Filippini Perla P Department of Systems Medicine, IRCCS San Raffaele Pisana, Rome, - - 2014
The efficacy of allogeneic hematopoietic stem cell transplantation is counterbalanced by the occurrence of life-threatening immune-mediated complications, such as graft-versus-host disease (GVHD), a multistep disease which is reportedly fatal to approximately 15% of transplant recipients. It is now established that T-cell-dendritic cell interactions, T-cell activation, release of proinflammatory cytokines and ...
Zang Wenqiao W College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, Zhengzhou, 450001, - - 2014
Myricetin, a common dietary flavonoid, is widely distributed in fruits and vegetables and is used as a health food supplement based on its anti-tumor properties. However, the effect and mechanisms of myricetin in esophageal carcinoma are not fully understood. Here, we demonstrated the effect of myricetin on the proliferation, apoptosis, ...
Tatsumi K K Department of Medicine, Division of Hematology and Oncology, UNC McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, - - 2014
Microparticles (MPs) are small membrane vesicles that are released by activated cells, such as monocytes, cells undergoing apoptosis and tumor cells [1]. Full-length tissue factor (flTF) is a transmembrane receptor for FVII/VIIa and is essentially undetectable in the blood of healthy individuals. However, intravascular levels of flTF are increased in ...
You Jijun J Department of Cardiothoracic Surgery, Taizhou Second People's Hospital, No. 27 Jiankang Road, Taizhou, 225599, - - 2014
Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. Previous studies have reported that loss of BRMS1 expression correlates with tumor progression, and poor prognosis in NSCLC. However, the role of BRMS1 in NSCLC is not fully understood. In this study, ...
Hind Laurel E LE Department of Bioengineering, University of Pennsylvania, Philadelphia, - - 2014
The ability of macrophages to migrate to sites of infection and inflammation is critical for their role in the innate immune response. Macrophage cell lines have made it possible to study the roles of individual proteins responsible for migration using molecular biology, but it has not been possible to reliably ...
Chao Ting-Ting TT - - 2014
Some patients with non-small cell lung cancer (NSCLC) without EGFR mutations still respond to gefitinib and erlotinib, suggesting that there may be mechanism(s) other than the EGFR-pathway that mediates the tumoricidal effects. In the current study, we tested the efficacy of TD-19, a novel compound chemically modified from erlotinib, which ...
Nakamura Morihiko M Department of Cooperative Medical Research, Collaboration Center, Shimane University , Izumo , - - 2014
Monoclonal non-specific suppressor factor β (MNSFβ) is a ubiquitously expressed member of the ubiquitin-like family that is involved in various biological functions. Previous studies have demonstrated that MNSFβ covalently binds to intracellular pro-apoptotic protein Bcl-G and regulates apoptosis in macrophages. In this study, we demonstrate that MNSFβ negatively regulates T ...
De Simone V V Department of Systems Medicine, University of Rome 'Tor Vergata', Rome, - - 2014
Colorectal cancers (CRCs) often show a dense infiltrate of cytokine-producing immune/inflammatory cells. The exact contribution of each immune cell subset and cytokine in the activation of the intracellular pathways sustaining CRC cell growth is not understood. Herein, we isolate tumor-infiltrating leukocytes (TILs) and lamina propria mononuclear cells (LPMCs) from the ...
Ashok Devika D Department of Biochemistry CIIL, University of Lausanne, 155 Chemin des Boveresses, 1066 Epalinges, - - 2014
Mouse models of Leishmania major infection have shown that a predominant CD4(+) T helper type 1 cell (Th1) response leads to protection, while T helper type 2 cell (Th2) predominance confers susceptibility. Dendritic cells (DCs) are antigen-presenting cells that orchestrate the T cell response. The immune response to L. major ...
Cincin Zeynep Birsu ZB Department of Molecular Medicine, Istanbul University, Institute of Experimental Medical Research, Capa, Istanbul, - - 2014
Quercitrin (QR; quercetin-3-O-rhamnoside) has been used previously as an antibacterial agent and has been shown to inhibit the oxidation of low-density lipoproteins and prevent an allergic reaction. Furthermore, it was demonstrated that quercitrin exerts protective effects against H2O2-induced dysfunction in lung fibroblast cells. However, the mechanisms of quercitrin effects on ...
Prud'homme Gérald J GJ Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Ontario, Canada; Department of Laboratory Medicine, St. Michael's Hospital, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada. Electronic address: - - 2014
We have previously shown that GABA protects pancreatic islet cells against apoptosis and exerts anti-inflammatory effects. Notably, GABA inhibited the activation of NF-κB in both islet cells and lymphocytes. NF-κB activation is detrimental to beta cells by promoting apoptosis. However, the mechanisms by which GABA mediates these effects are unknown. ...
Delloye-Bourgeois Céline C Apoptosis, Cancer and Development Laboratory- Equipe labellisée 'La Ligue', Centre de Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008 Lyon, - - 2014
Cell-adhesion molecule-related/Downregulated by Oncogenes (CDO or CDON) was identified as a receptor for the classic morphogen Sonic Hedgehog (SHH). It has been shown that, in cell culture, CDO also behaves as a SHH dependence receptor: CDO actively triggers apoptosis in absence of SHH via a proteolytic cleavage in CDO intracellular ...
Jin Liliang L Laboratory of Lung Biology, Department of Pathobiological Sciences, Center for Experimental Infectious Disease Research, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA - - 2014
Severe bacterial sepsis leads to a proinflammatory condition that can manifest as septic shock, multiple organ failure, and death. Neutrophils are critical for the rapid elimination of bacteria; however, the role of neutrophil chemoattractant CXCL1 in bacterial clearance during sepsis remains elusive. To test the hypothesis that CXCL1 is critical ...
Bake Vanessa V Institute for Experimental Cancer Research in Pediatrics, Goethe-University Frankfurt, Komturstr. 3a, 60528 Frankfurt, - - 2014
Therapeutic targeting of Inhibitor of Apoptosis (IAP) proteins by small-molecule inhibitors such as Smac mimetic is considered as a promising anticancer strategy to elicit apoptosis. Recent advances have renewed the interest in exploiting the antileukemic activity of interferon (IFN)α for the treatment of acute myeloid leukemia (AML). Here, we identify ...
Cowley Siobhán C SC Division of Bacterial Parasitic and Allergenic Products, Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 1401 Rockville Pike HFM-431, Rockville, MD, 20852, USA, - - 2014
Mucosa-associated invariant T (MAIT) cells are a unique population of innate T cells that are abundant in humans. These cells possess an evolutionarily conserved invariant T cell receptor α chain restricted by the nonpolymorphic class Ib major histocompatibility (MHC) molecule, MHC class I-related protein (MR1). The recent discovery that MAIT ...
Reynolds Ben C BC MRC Centre for Inflammation Research, University of Edinburgh, Edinburgh, United - - 2014
Interest in manipulating the immunosuppressive powers of Foxp3-expressing T regulatory (Treg) cells as an immunotherapy has been tempered by their reported ability to produce pro-inflammatory cytokines when manipulated in vitro, or in vivo. Understanding processes that can limit this potentially deleterious effect of Treg cells in a therapeutic setting is ...
Shigeishi Hideo H Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK; Department of Oral and Maxillofacial Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, - - 2014
Cancer stem cells (CSCs) are involved in both tumourigenesis and in tumour recurrence after therapy. In head and neck squamous cell carcinoma (HNSCC), there are two biologically different CSC phenotypes both of which express high levels of CD44 but differ in their expression levels of epithelial-specific antigen (ESA). One phenotype ...
Soulie Priscilla P University of Geneva Medical - - 2014
Generation of branched tubes from an epithelial bud is a fundamental process in development. We hypothesized that induction of hyaluronan synthase (Has) and production of hyaluronan (HA) drives branching tubulogenesis in response to morphogenetic cytokines. Treatment of J3B1A mammary cells with TGF-β1 or renal MDCK and mCCD-N21 cells with HGF ...
Sun Zhengwen Z Department of Traumatology, Yantaishan Hospital, Yantai, 264025, Shandong Province, - - 2014
Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstrated that miR-202 expression ...
Ou Yun-Wei YW 1] Department of Neurosurgery, Chinese PLA General Hospital, Beijing 100853, China [2] State Key Laboratory of Molecular Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, - - 2014
Aim:Mig-2 (also known as Kindlin-2 and FERMT2) is an important regulator of integrin activation and cell-extracellular matrix adhesion, and involved in carcinogenesis and tumor progression. The aim of this study was to investigate the role of mig-2 in cisplatin-induced apoptosis of human glioma cells in vitro.Methods:The expression of mig-2 was ...
Leplina Olga Yu OY Institute of Clinical Immunology SB RAMS, 14 Yadrintsevskaya Str., 630099 Novosibirsk, - - 2014
Dendritic cell-based vaccines are considered as a new and promising immunotherapeutic approach for cancer treatment. However, the choice of optimal protocol of dendritic cell generation in vitro represents the major challenge. Here, we compared phenotype and functional characteristics of human monocyte-derived dendritic cells (DCs) generated in the presence of IL-4/GM-CSF ...
Jovanović Ivan I University of Niš, Medical Faculty, Department of Anatomy, Dr Zoran Djindjić 81 Blvd., 18000 Niš, Serbia. Electronic address: - - 2014
Some evidence has suggested that, with age, the hypothalamic-pituitary-adrenal (HPA) axis becomes less resilient, leading to higher glucocorticoids nocturnal levels and a flattening of the circadian profiles. Such age-related changes in the activity of the HPA axis has overexposed the brain and peripheral organs to the effects of the glucocorticoids, ...
Humphries F F Department of Biology, Institute of Immunology, National University of Ireland Maynooth, Maynooth, County Kildare, - - 2014
Innate immunity represents the first line of defence against invading pathogens. It consists of an initial inflammatory response that recruits white blood cells to the site of infection in an effort to destroy and eliminate the pathogen. Some pathogens replicate within host cells, and cell death by apoptosis is an ...
Shen Yue Y Department of Orthopedic Surgery, School of Medicine, The Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China, - - 2014
In previous studies, selenium (Se) was reported to play critical roles in anti-inflammatory activities. Nevertheless, limited information could be obtained during inflammation about selenomethionine (SeMet) in U937 human macrophage cells. The purpose of this study was to investigate the effects of SeMet on the inflammatory responses to lipopolysaccharide (LPS)-induced U937 ...
Wang Haiying H Department of Spinal Surgery, Hebei Provincial Key Laboratory of Orthopaedic Biomechanics, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. - - 2014
Interleukin‑1β (IL‑1β) is a pleiotropic cytokine that mediates inflammatory and cell death activities. IL‑1β has been previously reported to induce apoptosis of intervertebral disc (IVD) cells in IVD degeneration. Accumulating data have suggested that post‑menopausal women have a high incidence of IVD degeneration. It has therefore been proposed that estrogen ...
Zhang Yubao Y Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong, P.R. - - 2014
Honokiol [3,5-di-(2-propenyl)-1,1-biphenyl-2,2-diol; HNK], a natural bioactive molecular compound isolated from the Magnolia officinalis, exhibits potent antitumor activity against a variety of human cancer cell lines. However, few studies have reported the antineoplastic effects of HNK on glioblastoma cells. It remains unknown how apoptosis is induced by HNK in glioblastoma cells ...
Liu Ying Y Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, - - 2014
Inflammatory cytokines are secreted by immune cells in response to infection or injury. Quantification of multiple cytokines in parallel may help with disease diagnosis by illuminating inflammatory pathways related to disease onset and progression. This paper describes development of an electrochemical aptasensor for simultaneous detection of two important inflammatory cytokines, ...
Zhu Xinhui X Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong, People's Republic of China, 226001. Electronic address: - - 2014
Chemotherapy has significantly improved the prognosis of high-grade osteosarcoma (OS), but over 30% of OS patients can still not be cured. Pemetrexed, the newly-developed anti-folate chemotherapy drug, exerted lower efficacy against OS cells. Here, we aimed to increase pemetrexed efficiency, and found that the cell-permeable short-chain ceramide (C6) significantly enhanced ...
Su Chang C School of Veterinary Medicine, Liaoning Medical University, Jinzhou 121000, PR - - 2014
Currently, clinical operation treatments, chemotherapy and radiotherapy just could eliminate local tumor cells. However, chemotherapy and radiotherapy also injury normal cells lead to serious side effects and toxicities. So, it is necessary to find an effective target cancer carrier that delivers the anticancer agents into tumor cells and reduces normal ...
Domínguez-Nieto Aimée A Departamento de Inmunogenética y Alergia, Instituto Nacional de Enfermedades Respiratorias (INER), Ismael Cosío Villegas, Mexico City, - - 2014
Prior exposure to lipopolysaccharides (LPS) induces a state of cell resistance to subsequent LPS restimulation, known as endotoxin tolerance, mainly by repressing the expression of pro-inflammatory cytokines. We established an endotoxin tolerance model in human monocytes Endotoxin-tolerant cells showed a decrease in IκBα degradation and diminished expression of Tumor necrosis ...
Kroner Antje A Centre for Research in Neuroscience, The Research Institute of the McGill University Health Center, 1650 Cedar Avenue, Montreal, Quebec, H3G 1A4, - - 2014
Macrophages and microglia can be polarized along a continuum toward a detrimental (M1) or a beneficial (M2) state in the injured CNS. Although phagocytosis of myelin in vitro promotes M2 polarization, macrophage/microglia in the injured spinal cord retain a predominantly M1 state that is detrimental to recovery. We have identified two ...
Liu Yun Y Division of Spinal Surgery, 1st Affiliated Hospital of Guangxi Medical University, Nanning, - - 2014
Background: Previous studies have shown that some phytoestrogens inhibits proliferation and induces apoptosis in estrogen-dependent cancers via estrogen receptor (ER)-mediated signaling pathway. In view of the expression of ER in human osteosarcoma cells, the purpose of this study is to investigate whether formononetin and calycosin, two of the major isoflavones ...
Neumann Ariane A Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, - - 2014
The formation of neutrophil extracellular traps (NETs) as a host innate immune defence mechanism has been shown to be the result of a novel cell death process called NETosis. The objective of this study was to investigate the role of cholesterol in the formation of NETs. To this end, primary ...
Schwab Jan M JM Department of Neurology and Experimental Neurology, Clinical and Experimental Spinal Cord Injury Research (Neuroparaplegiology), Charite-Universitatsmedizin Berlin, D-10117 Berlin, Germany; Spinal Cord Injury Center, Trauma Hospital Berlin, D-12683 Berlin, Germany. Electronic address: - - 2014
During the transition from acute to chronic stages of recovery after spinal cord injury (SCI), there is an evolving state of immunologic dysfunction that exacerbates the problems associated with the more clinically obvious neurologic deficits. Since injury directly affects cells embedded within the "immune privileged/specialized" milieu of the spinal cord, ...
Jones T Bucky TB Department of Anatomy, Arizona College of Medicine, Midwestern University, Glendale, AZ, USA. Electronic address: - - 2014
Over the past 15years an immense amount of data has accumulated regarding the infiltration and activation of lymphocytes in the traumatized spinal cord. Although the impact of the intraspinal accumulation of lymphocytes is still unclear, modulation of the adaptive immune response via active and passive vaccination is being evaluated for ...
Bastien Dominic D Centre de recherche du Centre hospitalier universitaire de Québec-CHUL, Département de médecine moléculaire, Université Laval, Québec, QC, - - 2014
Injury to the nervous system causes the almost immediate release of cytokines by glial cells and neurons. These cytokines orchestrate a complex array of responses leading to microgliosis, immune cell recruitment, astrogliosis, scarring, and the clearance of cellular debris, all steps that affect neuronal survival and repair. This review will ...
Plemel Jason R JR Department of Clinical Neurosciences, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, - - 2014
Historically, the immune response after spinal cord injury was considered largely detrimental owing to the release of neurotoxic factors. While there is validity to this view, there is much greater heterogeneity of immune cells than was previously realized. Associated with this heterogeneity of immune cell subtypes, there is diversity of ...
Peterson Sheri L SL Sue & Bill Gross Stem Cell Center, University of California, Irvine, Irvine, CA 92697, USA; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92697, USA; Department of Anatomy & Neurobiology, University of California, Irvine, Irvine, CA 92697, - - 2014
The pathology associated with spinal cord injury (SCI) is caused not only by primary mechanical trauma, but also by secondary responses of the injured CNS. The inflammatory response to SCI is robust and plays an important but complex role in the progression of many secondary injury-associated pathways. Although recent studies ...
Schopohl P - - 2014
Monocytes play a major role in modulating inflammation and can be found as circulating blood cells or, after extravasation, as differentiated tissue specific macrophages. The activation of TLRs (Toll-like receptors) modulates their action and contributes considerably to the eradication of pathogens. Aim of this study was to investigate the influence ...
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