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Ihlefeld Katja K Klinikum der Goethe-Universitaet, - - 2014
Sphingosine 1-phosphate (S1P) is an extra- and intracellular mediator that regulates cell growth, survival, migration and adhesion in many cell types. S1P lyase is the enzyme which irreversibly cleaves S1P and thereby constitutes the ultimate step in sphingolipid catabolism. It has been reported previously that embryonic fibroblasts from S1P lyase-deficient ...
Schinnerling Katina K Medizinische Klinik I, Charité-Universitätsmedizin Berlin, CBF, Berlin, - - 2014
Accumulation of Tropheryma (T.) whipplei-stuffed macrophages in the duodenum, impaired T. whipplei-specific Th1 responses and weak secretion of IL-12 are hallmarks of classical Whipple's disease (CWD). This study addresses dendritic cell (DC) functionality during CWD. We documented composition, distribution and functionality of DC ex vivo or after in vitro maturation ...
Nakagawa Akihisa A Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado, - - 2014
Inactivation of cell-survival factors is a crucial step in apoptosis. The phosphoinositide 3-kinase (PI3K)-AKT signaling pathway promotes cell growth, proliferation and survival, and its deregulation causes cancer. How this pathway is suppressed to promote apoptosis is poorly understood. Here we report the identification of a CED-3 caspase substrate in Caenorhabditis ...
Yuan Jing J Department of Hematology, Peking University First Hospital, 8 Xishiku Street, Xicheng District, 100034, Beijing, - - 2014
Blockade of CC chemokine receptor 5 (CCR5) by maraviroc may induce immunological changes independent of its antiviral effects and may have immunoregulation properties. This study was designed to determine the effects of blocking CCR5 on human activated T cells in vitro and investigate the potential immunological mechanisms. Human CD3+ T ...
Lee Sue Hyun SH Department of Biomedical Engineering, Vanderbilt University, Nashville TN 37235, USA. Tel: - - 2014
A reactive oxygen species (ROS)-degradable scaffold is fabricated by crosslinking biocompatible, hydrolytically-degradable poly(ε-caprolactone) (PCL) with a ROS-degradable oligoproline peptide, KP7K. The ROS-mediated degradability triggers favorable host responses of the scaffold including improved cell infiltration and angiogenesis in vivo, indicating its unique advantages for tissue engineering applications.
Sauter Alexander A Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5021, Bergen, - - 2014
Ex vivo generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell-culture dishes. In order to elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with 4 different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 ...
Liu Bin B Laboratory of Hepatobiliary Surgery, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. - - 2014
Dihydromyricetin (DHM) is a flavonoid compound which possesses potent antitumor activity. In the present study, it was demonstrated that DHM significantly inhibited proliferation and induced apoptosis in mouse hepatocellular carcinoma Hepal‑6 cells. Transforming growth factor β (TGF‑β) is recognized as a major profibrogenic cytokine and is therefore a common target ...
Chao Pin-Zhir PZ Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Department of Otolaryngology, Shuang-Ho Hospital, New Taipei, - - 2014
Dendritic cells (DCs) play an essential role in immunity and are used in cancer immunotherapy. However, these cells can be tuned by tumors with immunosuppressive responses. DC-specific intercellular adhesion molecule 3-Grabbing Nonintegrin (DC-SIGN), a C-type lectin expressed on DCs, recognizes certain carbohydrate structures which can be found on cancer cells. ...
Bai Hualong H Vascular Biology and Therapeutics Program and the Department of Surgery, Yale University School of Medicine, New Haven, Conn; Basic Medical College of Zhengzhou University, Henan, China; Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, - - 2014
Pretreatment with antibiotics is commonly performed before surgical implantation of prosthetic materials. We previously showed that pericardial patches are infiltrated by macrophages and arterial stem cells after implantation into an artery. We hypothesized that antibiotic pretreatment would diminish the number of cells infiltrating into the patch, potentially affecting early neointimal ...
Xu Gong-Ping GP Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, 148 Baojian Road, Harbin, 150081, - - 2014
Matrine, a natural product, has been demonstrated to be a promising chemotherapeutic drug for some cancers. Using flow cytometric analysis of the cell cycle and apoptosis, we found that matrine inhibited the proliferation and induced apoptosis in the human osteosarcoma (OS) cell lines MG63, HOS, U2OS, and SAOS2 in vitro ...
Karumuthil-Melethil Subha S Department of Surgery, University of Illinois at Chicago, Chicago, - - 2014
The progression of autoimmune diseases is dictated by deviations in the fine balance between pro-inflammatory versus regulatory responses and pathogen recognition receptors (PRRs) play a key role in maintaining the balance. Previously, we have reported that ligation of TLR2 and Dectin 1 on APCs by zymosan results in a regulatory ...
Audrito Valentina V Department of Medical Sciences, University of Torino, - - 2014
Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in NAD biosynthesis. In the extracellular compartment it exhibits cytokine/adipokine-like properties, suggesting that it stands at the crossroad between metabolism and inflammation. Here we show that both intracellular and extracellular NAMPT levels are increased cells and plasma of chronic lymphocytic leukemia (CLL) patients. ...
El-Mesery Mohamed M Division of Molecular Internal Medicine, Department of Internal Medicine II, University Hospital Würzburg; Department of Biochemistry, Faculty of Pharmacy, Mansoura University, - - 2014
MLN4924 prevents the formation of active cullin-RING ubiquitin ligase complexes and thus inhibits NFκB signaling. Here, we evaluated the effects of this compound on monocytes and dendritic cells (DCs). Monocytes and DCs were challenged with TNF or LPS in the presence and absence of MLN4924. The effects of MLN4924 on ...
Nam Kyung-Soo KS Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju, Gyeongsangbuk-do 780-714, Republic of - - 2015
Colorectal adenocarcinoma is the most common type of gastrointestinal cancer. Colon adenocarcinoma is a major health problem worldwide due to the high prevalence and mortality rates associated with the disease. The majority of colorectal carcinomas are adenocarcinomas, which originate from the epithelial cells of the colorectal mucosa. HT-29 cells, which ...
Dutta Partha P Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, - - 2014
Monocytes are part of the vertebrate innate immune system. Blood monocytes are produced by bone marrow and splenic progenitors that derive from hematopoietic stem cells (HSCs). In cardiovascular disease, such as atherosclerosis and myocardial infarction, HSCs proliferate at higher levels that in turn increase production of hematopoietic cells, including monocytes. ...
Wang John J Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University Langone Medical Center, New York, NY 10016, - - 2014
Chemokines are a group of small, secreted molecules that signal through G protein-coupled receptors to promote cell survival and proliferation and to provide directional guidance to migrating cells. CXCL12 is one of the most evolutionary conserved chemokines and signals through the chemokine receptor CXCR4 to guide cell migration during embryogenesis, ...
Gao Xue Juan XJ Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, Guangdong, - - 2014
The protozoan Toxoplasma gondii expresses large amounts of a 37 kDa Type 2C serine-threonine phosphatase, the so-called TgPP2C which has been suggested to contribute to parasite growth regulation. Ectopic expression in mammalian cells also indicated that the enzyme could regulate growth and survival. In this study, we aimed to investigate ...
Gianni Tatiana T Department of Experimental, Diagnostic and Specialty Medicine, Alma Mater Studiorum-University of Bologna, Bologna, - - 2014
TLR2 is a cell surface receptor which elicits an immediate response to a wide repertoire of bacteria and viruses. Its response is usually thought to be proinflammatory rather than an antiviral. In monocytic cells TLR2 cooperates with coreceptors, e.g. CD14, CD36 and αMβ2-integrin. In an earlier work we showed that ...
Escudero-Pérez Beatriz B Molecular Basis of Viral Pathogenicity, CIRI, INSERM U1111- CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon, - - 2014
During Ebola virus (EBOV) infection a significant amount of surface glycoprotein GP is shed from infected cells in a soluble form due to cleavage by cellular metalloprotease TACE. Shed GP and non-structural secreted glycoprotein sGP, both expressed from the same GP gene, have been detected in the blood of human ...
Suzuki-Yamamoto Toshiko T Department of Nutritional Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, - - 2014
Prostaglandin E2 plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. Prostaglandin E2 is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin E synthase. In some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin E synthase-1 are transiently induced, leading to prostaglandin ...
Rottenberg Martin E ME Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, S-171 77 Stockholm, Sweden. Electronic address: - - 2014
In our review, we address the role of signal transducer and activator of transcription-3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) in the outcome of Mycobacterium tuberculosis infection, focusing on functions of these molecules in regulating the biology of myeloid and lymphoid cells. The STAT3 transcription factor has paradoxical roles: ...
Lee Hyun Gyo HG Institute for Cancer Research, Keimyung University School of Medicine, Daegu, - - 2014
The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. ...
Tripathi Sudipta - - 2014
Tolerance of the fetus by the maternal immune system is regulated through various mechanisms involving the different immune cells, both in the periphery and locally at the feto-maternal interface. The maternal T lymphocytes are aware of the paternal fetal antigens and a state of dynamic T cell homeostasis is maintained ...
Epanchintsev Alexey A Institute of Genetics and Molecular and Cellular Biology, Department of Functional Genomics and Cancer Biology, Centre National de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale Illkirch Cedex, - - 2014
Fanconi anemia (FA) is a rare autosomal recessive genetic disorder associated with a bone-marrow failure, genome instability, hypersensitivity to DNA crosslinking agents and a predisposition to cancer. Mutations have been documented in 16 FA genes that participate in the FA-BRCA DNA repair pathway, a fundamental pathway in the development of ...
Schögler Aline A Division of Paediatric Respiratory Medicine, University Children's Hospital, Berne, Switzerland Dept of Clinical Research, University of Berne, Berne, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Berne, Berne, - - 2014
Virus-associated pulmonary exacerbations, often associated with rhinoviruses (RVs), contribute to cystic fibrosis (CF) morbidity. Currently, there are only a few therapeutic options to treat virus-induced CF pulmonary exacerbations. The macrolide antibiotic azithromycin has antiviral properties in human bronchial epithelial cells. We investigated the potential of azithromycin to induce antiviral mechanisms ...
Hao Hua H Department of Pathology, The Second Affiliated Hospital of Nanchang University, No. 1 MinDe Road, Jiangxi Province, Nanchang City, 330006, China, - - 2014
Uterine cervical carcinoma (UCC) is one of the most common malignant tumors in females, and UCC has a close relationship with chronic cervicitis. As the endogenous "braking signal," lipoxins can regulate anti-inflammation and the resolution of inflammation. We investigated the effect of lipoxin A4 (LXA4) on the proliferation, apoptosis, and ...
Park Sang Yoon SY NICHD/NIH, United - - 2014
Vpu is an accessory protein encoded by HIV-1 that interferes with multiple host-cell functions. Herein we report that expression of Vpu by transfection into 293T cells causes partial proteolytic cleavage of interferon regulatory factor 3 (IRF3), a key transcription factor in the innate anti-viral response. Vpu-induced IRF3 cleavage is mediated ...
Ansary Md Mesbah Uddin MM *Department of Internal Medicine II, School of Medicine, Shimane University, Izumo, Japan; †Department of Gastrointestinal Endoscopy, Shimane University Hospital, Izumo, Japan; and ‡Research Division, JIMRO Co. Ltd., Takasaki, - - 2014
: Apoptosis is a programmed physiological death of unwanted cells, and handling of apoptotic cells (ACs) is thought to have profound effects on immune-mediated disorders. However, there is scant information regarding the role of ACs in intestinal inflammation, in which immune homeostasis is a major concern. To investigate this, we ...
Ni Pan - - 2014
Cisplatin is one of the most commonly used in the treatment of gastric cancer. However, drug resistance is a major obstacle for effective treatment and originates in multiple mechanisms such as enhanced DNA repair and anti-apoptosis. Our previous results demonstrated that XRCC1 was a key regulator of cisplatin induced DNA ...
Jung Joo-Yong JY Department of Biology, Briar Cliff - - 2014
Dendritic cells (DCs) are potent antigen presentation cells necessary to establish effective adaptive immune responses. The cytokine environment that exists at the time of DC differentiation may be an important but often ignored determinant in the phenotypic and functional properties of DCs. Interleukin (IL)-27 is a unique cytokine that has ...
Qi Suqin S MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China. Electronic address: - - 2014
Bisphenol-A was examined for its effects on cultured Sertoli cells established from 18 to 22-day-old rat testes. Results indicated that exposure to BPA (0, 30, 50 and 70μM) decreased the cell viability in a concentration-dependent manner and induced cell apoptosis. Apoptosis-caused cell death was observed in cells exposed to 50 ...
Jayasooriya Rajapaksha Gedara Prasad Tharanga RG Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of - - 2014
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in various types of malignant cancer cells, but several cancers have acquired potent resistance to TRAIL-induced cell death by unknown mechanisms. Camptothecin (CPT) is a quinolone alkaloid that induces cytotoxicity in a variety of cancer cell lines. However, it is not known ...
Zhou Meng M Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing, Jiangsu Province, People׳s Republic of - - 2014
Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current study, we investigated the role of ...
Lakshmikuttyamma A - - 2014
siRNA is currently the most widely studied form of RNAi, and has a promising therapeutic potential in not just cancer but in other diseases such as autoimmune and infectious diseases. However, efficient delivery of siRNA to target cells is being limited by lack of an effective delivery system that ensures ...
Petri Marcelo H MH Experimental Cardiovascular Research - - 2014
The formyl peptide receptor (FPR) subtype FPR2/ALX transduces pro-inflammatory responses and participates in the resolution of inflammation depending on activation. The aim of the present study was to unravel the role of FPR2/ALX signaling in atherosclerosis. Expression of FPR2/ALX was analyzed in 127 human carotid atherosclerotic lesions and revealed that ...
Mori Masaki M Department of Orthopaedic Surgery, Kagawa University School of Medicine, Miki‑cho, Kagawa 761‑0793, - - 2014
Heat shock protein 90 (Hsp90) is constitutively expressed at 2‑10‑fold higher levels in tumor cells compared to normal cells, suggesting that it may be critically important for tumor cell growth and survival. These features make Hsp90 a potential target for anticancer drug development. Inhibition of Hsp90 activity not only results in rapid ...
Mauney Joshua R JR Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA; Department of Surgery, Harvard Medical School, Boston, MA 02115, - - 2014
Tissue engineering in urology has shown considerable promise. However, there is still much to understand, particularly regarding the interactions between scaffolds and their host environment, how these interactions regulate regeneration and how they may be enhanced for optimal tissue repair. In this review, we discuss the concept of dynamic reciprocity ...
Zhou Yan Y Life Sciences Institute and Innovation Center for Cell Biology, Zhejiang University, Hangzhou, Zhejiang 310058, - - 2014
Ubiquitination is generally considered as a eukaryotic protein modification, which is catalyzed by a three-enzyme cascade and is reversed by deubiquitinating enzymes. Ubiquitination directs protein degradation and regulates cell signaling, thereby plays key roles in many cellular processes including immune response, vesicle trafficking and cell cycle. Bacterial pathogens inject a ...
Xu Hao H Department of Surgery, Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee, WI, United - - 2014
High Mobility Group Box 1 (HMGB1) is a chromatin binding protein that maintains DNA structure. Upon cellular activation or injury, HMGB1 is released from activated immune cells or necrotic tissues and acts as a Damage-Associated Molecular Pattern (DAMP) to activate Toll-Like Receptor 4 (TLR4). Very little is known concerning HMGB1 ...
Luan Zhou Z Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. - - 2014
The enhancement of apoptosis is a therapeutic strategy used in the treatment of cancer. Tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) is a promising antitumor agent. However, hepatocellular carcinoma (HCC) cells exhibit marked resistance to the induction of cell death by TRAIL. The present study investigated whether rocaglamide, a naturally occurring ...
Zhang Jie J College of Science and Technology, Nihon University, 1-8-14 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, - - 2014
The MeOH extract of defatted shea (Vitellaria paradoxa; Sapotaceae) kernels was investigated for its constituents, and fifteen oleanane-type triterpene acids and glycosides, two steroid glucosides, two pentane-2,4-diol glucosides, seven phenolic compounds, and three sugars, were isolated. The structures of five triterpene glycosides were elucidated on the basis of spectroscopic and ...
Rosser Elizabeth C EC Centre for Rheumatology, Division of Medicine, University College London, London, - - 2014
Regulatory B cells (Breg cells) differentiate in response to inflammation and subsequently restrain excessive immune responses via the release of interleukin-10 (IL-10). However, the precise inflammatory signals governing their differentiation remain to be elucidated. Here we show that the gut microbiota promotes the differentiation of Breg cells in the spleen ...
Chen Mao-Liang ML Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation, New Taipei City, - - 2014
Antipsychotic drugs (APDs) have been used to ease the symptoms of schizophrenia. APDs have recently been reported to regulate the immune response. Our previous studies revealed that the atypical APDs risperidone and clozapine and the typical APD haloperidol can inhibit the phagocytic ability of macrophages. Our research next determined the ...
Kim Dae Sung DS Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, - - 2014
Plants use a variety of innate immune regulators to trigger cell death and defense responses against pathogen attack. We identified pepper (Capsicum annuum) GLYCINE-RICH RNA-BINDING PROTEIN1 (CaGRP1) as a RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 (CaPIK1)-interacting partner, based on bimolecular fluorescence complementation and coimmunoprecipitation analyses as well as gene silencing and transient ...
Moraco Andrew H AH Department of Medicine, University of Massachusetts Medical School, Worcester, MA, - - 2014
Mycobacterium tuberculosis has succeeded in infecting one-third of the human race though inhibition or evasion of innate and adaptive immunity. The pathogen is a facultative intracellular parasite that uses the niche provided by mononuclear phagocytes for its advantage. Complex interactions determine whether the bacillus will or will not be delivered ...
Xu Ye Y Medical Research Laboratory, Jilin Medical College, Changchun, Jilin 130021, P.R. - - 2014
Cisplatin is commonly used as a therapeutic agent, despite its known adverse side effects and the occurrence of drug resistance. The development of novel methods for combination therapy with cisplatin is required in order to circumvent these limitations of cisplatin alone. The proteasome inhibitor lactacystin (LAC) has been indicated to ...
Hu Ruozhen R Department of Pathology, Division of Microbiology and Immunology, University of Utah, 4280 JMRB, 15 North Medical Dr. East, Salt Lake City, UT 84112, - - 2014
Chronic inflammation is a contributing factor to most life-shortening human diseases. However, the molecular and cellular mechanisms that sustain chronic inflammatory responses remain poorly understood, making it difficult to treat this deleterious condition. Using a mouse model of age-dependent inflammation that results from a deficiency in miR-146a, we demonstrate that ...
Lee Eunjung E Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, South - - 2014
Cecropin A is a novel 37-residue cecropin-like antimicrobial peptide isolated from the cecropia moth, Hyalophora cecropia. We have demonstrated that cecropin A is an antibacterial agent and have investigated its mode of action. In this study, we show that cecropin A has potent antimicrobial activity against 2 multidrug resistant organisms-Acinetobacter ...
Jin Zhishan Z Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. - - 2014
Ceramide is a bioactive lipid which functions as a tumor suppressor, mediating processes such as apoptosis, growth arrest, senescence and differentiation. The effects of ceramide in ovarian cancers have not been well established. The objective of the present study was to investigate the effects of C2‑ceramide treatment in A2780 ovarian ...
Chen Mao-Liang ML Department of Research, Taipei Tzuchi Hospital, The Buddhist Tzuchi Medical Foundation , New Taipei City, Taiwan , ROC - - 2014
Abstract Tournefortia sarmentosa, a Chinese herbal medicine, is considered an antioxidant or a detoxicating agent. Recently T. sarmentosa has received attention for its effects on the immune response. Here we provide evidence that aqueous extract of T. sarmentosa can induce increased phagocytic uptake of Escherichia coli by differentiated HL-60 cells ...
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