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Results 701 - 750 of 772
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Takata I - - 1985
Alveolar macrophages (AM), harvested from the lungs of untreated normal young rabbits (New Zealand White) 14 days to 8 weeks of age, exhibited a state of migration stimulation compared to AM from normal adult rabbits (5 to 6 months of age). Migration of AM from normal adult rabbits (New Zealand ...
Cathcart M K - - 1985
Free radicals are believed to be involved in leukocyte induced tissue injury. The present studies were performed to determine whether low density lipoprotein (LDL) might serve as a mediator of tissue injury after leukocyte induced free radical oxidation of LDL. Our results show that incubation of LDL with monocytes or ...
Liu D Y - - 1985
The role of carbohydrate in the interaction of human migration inhibitory factor (MIF) with human peripheral blood monocytes was investigated by studying the effects of different exoglycosidases on the cellular response to MIF. When monocytes were pretreated with neuraminidase, an exoglycosidase specific for sialic acid, they became unresponsive to MIF. ...
Prokić L M - - 1985
While the influence of 2-mercaptoethanol (2-ME) on the functions of lymphocytes has rather extensively been investigated, there are almost no data on the action of this thiol compound on macrophages. We examined the effect of 2-ME in vitro (10(-6)-10(-4) M) on migration from capillary tubes of oil elicited peritoneal macrophages ...
Schwartz C J - - 1985
With an increasing interest in the role of the monocyte-macrophage in the pathogenesis of atherosclerosis and as a progenitor of plaque intimal foam cells, a model for the study of foam-cell differentiation in an extravascular environment has been developed. Granulomas were induced in 25 normocholesterolemic (NC) and 28 hypercholesterolemic (HC) ...
Tabas I - - 1985
Cholesteryl ester (CE)-loaded macrophages (foam cells) are a prominent feature of atherosclerotic plaques. Previous studies have shown that human monocytes or resident mouse peritoneal macrophages accumulate CE in response to low density lipoprotein (LDL) only when the LDL has been appropriately chemically modified. By contrast, we report here that J774 ...
Higuchi M - - 1984
Human MAF-C (macrophage-activation factor for cytotoxicity)-producing hybridoma H2-E3-5 was prepared by somatic cell fusion of PHA-activated peripheral blood lymphocytes with emetine/actinomycin D-treated cloned human acute lymphatic leukemia cells (CEM). The following activities were assayed: (1) macrophage-migration-inhibitory factor (MIF), (2) macrophage-activation factor for glucose consumption (MAF-G), (3) macrophage-activation factor for O2-formation ...
Edwards R L - - 1984
Human monocytes generate the procoagulant tissue factor (MTF) following exposure to a variety of immune stimuli in vitro. The generation of MTF is modified by T cells, lymphokines, and immunoregulatory lipoproteins, and recent studies have shown that MTF can be activated in an immune-specific manner following exposure to antigen. We ...
Naukkarinen A - - 1984
The effects of rabbit anti-chicken macrophage serum (RACMS) on the immune response in chickens stimulated locally with sheep red-blood cells (SRBC; T cell-dependent antigen) were studied, and the morphological alterations in the diffusely infiltrated area (DIA; T cell region of the bursa) were measured morphometrically. The response to SRBC was ...
Hotez P J - - 1984
Lysates of the haematoprotozoa Trypanosoma brucei or Plasmodium berghei stimulated murine peritoneal exudate cells to release a mediator, which suppressed lipoprotein lipase activity in differentiating 3T3-L1 preadipocytes. The parasite-induced mediator suppressed the activity of cell surface lipoprotein lipase up to 39% in a dose dependent manner. By impairing the activity ...
Wolf R - - 1984
Drug induced allergic reactions in treated patients are sometimes accompanied by laboratory evidence of a simultaneous drug-specific humoral and cellular immunity. The relationship between the two types of immune response was studied in an experimental model. In the present studies ICR mice were sensitized with horse serum to induce an ...
Boswell J M - - 1984
Mononuclear phagocytes comprise populations of both stationary and wandering cells in vivo, and play many important roles, including major ones in host defence and the regulation of the immune response. They also interact with fibroblasts in the production and degradation of collagen and are therefore responsible in part for the ...
Khoo J C - - 1984
High levels of neutral triglyceride lipase activity have been demonstrated in several types of macrophages (J774 cells, human monocyte/macrophages, rabbit alveolar macrophages, and resident mouse peritoneal macrophages). The pH optima ranged from 6.5 to 7.4 depending upon the buffer and the conditions of incubation. The addition of bovine serum albumin ...
Sorg C - - 1984
It has been described before that only certain types of macrophages are capable to respond to lymphokines and that only certain macrophage phenotypes were able to migrate and to respond to migration inhibitory factors (MIF). With respect to the dissociation of MIF activities from a series of other biological activities, ...
Peck R - - 1984
Guinea pig lymph node cells stimulated with concanavalin A (Con-A) were used as a source of lymphokines. Purifications by molecular sizing columns yielded sufficient quantities of macrophage activating factor (MAF)-active fractions to allow immunization of rabbits. An antiserum was produced which is capable of specifically inhibiting MAF activity, as well ...
Peck R - - 1983
A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus detected correlate well with the migration as measured ...
Mizushima A - - 1983
The macrophage migration inhibitory factor (MIF) fraction was prepared from the immunoadsorbent column by using anti-guinea pig MIF antiserum. Suppression of cutaneous delayed-type hypersensitivity was achieved by intraperitoneal injection of the MIF fraction into the animals bearing macrophage-rich peritoneal exudates. Skin reactions induced by phytohemagglutinin (PHA) were also suppressed in ...
Wannamaker L W - - 1983
Few of the cellular components of group A streptococci appear to be directly toxic for animals or humans. Some preparations of M protein produce an immunotoxic effect on human platelets and neutrophils. Cell wall fragments produce a chronic multinodular inflammatory lesion of dermal connective tissue. The peptidoglycan component of cell ...
Leu R W - - 1983
Guinea pig macrophage populations previously established to be either responsive or refractory to activation by migration inhibitory factor (MIF) and Lotus fucolectin in the macrophage migration inhibition (MMI) assay were further characterized for their MMI response to diverse effectors as correlated with their Fc and C3b receptor function. MIF-refractory populations ...
Asada M - - 1983
Human lymphotoxin (LT)-producing T-cell hybridomas were constructed by fusing concanavalin A-activated human peripheral blood lymphocytes with emetine-actinomycin D-pretreated human acute lymphatic leukemia cells. LT secretion from these hybridomas was considerably enhanced by stimulation with phorbol-12-myristate-13-acetate (PMA) and concanavalin A or PMA alone. A study using cloned hybrid lines revealed that ...
Onozaki K - - 1983
The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocytize latex particles by ...
Kimura Y - - 1983
Bradykinin, as well as histamine, one of the mediators in IgE mediated immediate type allergic reactions or acute inflammation, may affect the in vitro and in vivo cell-mediated immune reactions of the immunized animals. It was demonstrated in our present experiment that the appearance of delayed type hypersensitivity (DTH) skin ...
Kahlich R - - 1983
A prospective study involving 20 volunteers was designed to test relationships between the sensitivity to zymosan and the indicators of nonspecific immunity against streptococci (inhibitory activity of blood and production of mediators). The immunization with Mannozym produced a conversion of zymosan-induced MIF with a subsequent improvement of immunity indicators in ...
Mazzone T - - 1982
Regulation of the macrophage receptor for modified low density lipoprotein (LDL) was evaluated using human monocyte-derived macrophages and acetyl LDL. Factors that regulate native LDL receptor activity in other cell types, such as the cholesterol content of the incubation medium, insulin, and platelet-derived growth factor had no effect on acetyl ...
Evans C H - - 1982
Biphasic modulation of the frequency of 3-methylcholanthrene-induced tumors in relation to increasing immunocompetence of tumor-bearing mice is indicative of lymphoresponsive stimulatory and inhibitory phases of carcinogenesis; components and mechanisms contributing to the paradoxical ability of the native or natural immune system to stimulate as well as to inhibit the development ...
Sud I J - - 1982
In Staphylococcus aureus, using the imidazoles miconazole and ketoconazole, detailed studies of minimal inhibitory concentrations, kinetics of growth, viability, and release of intracellular K+ confirm that the two imidazoles work differently in this bacterium. Miconazole is bactericidal at low concentrations and causes release of cellular K+. Ketoconazole has no bactericidal ...
Polack F M - - 1982
Leukocyte migration inhibition factor (LMIF) assays have been used as a measure of cell-mediated immune responses. Direct assays of this factor were determined in 30 patients with recurrent herpes simplex stromal keratitis during the quiescent stage of the disease and in 10 of these patients during the acute stage as ...
Sorg C - - 1982
Macrophage migration inhibitory factors (MIFs) of mouse and guinea pig have been thoroughly characterized with regard to molecular weight and isoelectric points. Several molecular weight species have been identified. In a comparative study with purified MIFs it was found that these molecules were distinct from a series of other lymphokines, ...
Livni E - - 1982
Macrophage migration inhibition factor (MIF) towards autologous seminal fluid was studied in 29 infertile men, 16 of whom were also examined towards normal seminal fluid. In 10 patients, inhibition of macrophage migration was observed in the presence of 50 microgram of protein of autologous semen sonicates. Nine patients had enhanced ...
Kahlich R - - 1982
Exoproducts of incubated white blood elements from persons hypersensitive to zymosan were found to promote the phagocytic activity of human microphages (and guinea pig macrophages) under conditions of a combined model of "MIF-opsonophagocytic test". The stimulation resulted in a bacteriostatic effect, was non-type-specific and improved microphage potential to inactivate streptococci ...
Bendtzen K - - 1982
The mechanism of action of the immunosuppressive drug, cyclosporin A (CyA), was analyzed with respect to antigen-induced production of the human lymphokine, leukocyte migration inhibitory factor (LIF). Mononuclear cells as well as purified T-cells did not elaborate LIF in the presence of pharmacological concentrations of CyA. CyA-induced immunosuppression was competitively ...
MacSween J M - - 1982
While the phenomenon of macrophage migration inhibition is a useful indicator of lymphokine release, it may be caused by other substances, it is subject to considerable variability, and it may be masked by the concomitant presence of substances stimulating migration. We have investigated certain aspects of the lymphokine macrophage interaction ...
Sukhtankar A Y - - 1982
Evaluation of cellular immunity in burns was undertaken using the following parameters: T-cell count; blastoid transformation and macrophage migration inhibition factor index (MIF). Their influence upon bactericidal index was also measured as T-cell count per mm3 and in percentage was found to be depressed in parallel, probably due to some ...
Traber M G - - 1981
IC21 macrophages, a permanent culture of a line of cells derived from a single colony of mouse peritoneal macrophages transformed with simian virus 40, demonstrate most of the characteristics of lipoprotein metabolism that have been described for primary cultures of rodent or canine peritoneal macrophages. IC21 macrophages have low but ...
Edgington T S - - 1981
Consideration of the effects of nutrition and of lipids on the immune competence and the characteristics of host resistance to neoplasia requires consideration of the plasma lipoproteins. A wide variety of regulatory effects of lipoproteins on the immune response, immune mediation pathways, cellular metabolism of the immune system, and antigen-nonspecific ...
Chisari F V - - 1981
Epstein-Barr virus (EBV)-induced immortalization of adult human B lymphocytes is suppressed by physiologic concentrations of human plasma lipoproteins. Several inhibitory mechanisms appear to be operative. First, low density lipoproteins (LDL) directly reduce the ability of EBV to transform human B cells. Second, LDL as well as intermediate and very low ...
Keogh R W - - 1981
A tricyclic chromone, proxicromil (sodium 6,7,8,9-tetrahydro-5-hydroxy-4-oxo-10-propyl-naphtho (2,3-b) pyran-2-carboxylate), has been tested for activity against certain immunological and inflammatory reactions. When given parenterally it suppressed the development of delayed hypersensitivity reactions in sensitized mice and guinea-pigs but did not affect the rejection of skin allografts in mice. The compound had no ...
Schwartz B S - - 1981
In the process of analyzing the effects of lipoproteins on functions of lymphoid cells, it was observed that physiological concentrations of isolated human plasma lipoproteins possess varying capacities to rapidly enhance the expression of procoagulant activity of human peripheral blood mononuclear cells in vitro. In a strict dose-dependent fashion, very ...
Traber M G - - 1980
Human peripheral monocytes, isolated from a previously unused source (a leukocyte concentrate byproduct of the plateletphoresis procedure for platelet transfusion), transformed into macrophages while cultured with 5% human serum or isolated lipoprotein fractions. Used for the study of their cholesterol (Chol) metabolism, these human monocyte-derived macrophages had a high-affinity receptor ...
Block L H - - 1980
The new antilymphocytic agent Cyclosporin A was found to inhibit the production and/or secretion of migration inhibitory factor (MIF) in human lymphocytes stimulated by Concanavalin A. Preincubation for one hour with the compound, followed by 8 hr restoration period of the cells in absence of the drug, resulted in moderate ...
Chensue S W - - 1980
Host granulomatous inflammation in murine schistosomiasis mansoni is a T cell-mediated immune response, which, at the chronic stage of the disease, undergoes T suppressor lymphocyte-dependent modulation. In the present study this phenomenon was further analyzed in vitro. Spleen cells of mice undergoing modulation (20 wk of infection) when mixed with ...
Liu D Y - - 1980
Acidic glycolipids from guinea pig macrophages enhance the response of macrophages to migration inhibitory factor (MIF), suggesting a role of glycolipid receptors for this lymphocyte mediator. Neuraminidase treatment of these glycolipids results in the loss of their biologic activity. This activity remains intact after incubation of the glycolipids with beta-galactosidase. ...
Varesio L - - 1980
We directly compared the effects of macrophages from infiltrating MSV-induced regressing tumors (T-M0) or from the peritoneal cavity of mice previously injected with light mineral oil (LMO-M0) on two functions of alloimmune spleen cells (ISC), the cytotoxic activity and production of a lymphokine, macrophage inhibitory factor (MIF). Addition of T-M0 ...
Kozłowska K - - 1980
The influence of two kinds of transplantable melanomas on the content of fucose in the surface glycoproteins of hamster peritoneal macrophages was studied. An increase in the content of fucose, more prominent in surface material of macrophages from animals with melanotic melanoma was found. It has been suggested that this ...
Sandberg G - - 1980
Migration of guinea pig thymocytes in sealed glass capillaries was measured in 3 intervals during 24 h of incubation. Two factors present in guinea pig sera which stimulated migration were partly characterized. One factor, with a molecular weight between 10,000 and 100,000 (as judged by ultrafiltration), stimulated migration during the ...
Sorg C - - 1979
Activated lymphocytes release numerous products which are either synthesized de novo or in increased amounts; some of these products play a role in the regulation of the immune response and are designated as mediators of cellular immune reactions or lymphokines. The first lymphokine described was the macrophage migration inhibitory factor ...
Possanza G - - 1979
Macrophage migration inhibition factor (MIF) derived from human lymphoid cell lines was found to lose biologic activity on dialysis. Although activity was not recovered in the dialyzate, mixing experiments demonstrated that the components in the retentate and dialyzate could reassociate to restore activity. The fragment of larger molecular weight (less ...
Varesio L - - 1979
Immune spleen cells from mice injected with murine sarcoma virus (MSV) can produce migration inhibition factor (MIF) in vitro after stimulation with intact tumor cells. We have now found that this effector function can be regulated by suppressor macrophages present in the tumor. The cells from the tumor (CfT) exert ...
Igarashi I - - 1979
When migration inhibitory factor (MIF) assay in vitro was conducted on the lymphokines (LKs), it was observed that the percentage of MIF activity was greatly increased from the 3rd to the 4th week postinfection. On the succeeding weeks there was a noticeable decrease in the MIF activity noted on the ...
Masih N - - 1979
Granulomatous reactions were immunologically induced in guinea pigs by several procedures, including intravenous injections of Bacille Calmette Gúerin (BCG) into animals immunized with complete Freund's Adjuvant and an intravenous injection of agarose beads linked to a specific antigen (dinitrophenylated bovine serum albumin) into immune animals. The tissue extracts obtained from ...
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