Search Results
Results 501 - 550 of 721
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Anniballo R - - 2000
In patients with non-obstructive azoospermia, testicular sperm extraction (TESE) is a method of choice to recover spermatozoa as a male therapeutic approach in intracytoplasmic sperm injection (ICSI) programmes. However, the efficacy of TESE in this indication is burdened by a frequent failure of sperm recovery, which renders useless both the ...
Brogliatti G M - - 2000
Ultrasound-guided transvaginal follicle aspiration has been described as a noninvasive and repeatable procedure for oocyte collection in several species, but its use has not been described for any of the members of the family, Camelidae. A study was designed to determine the feasibility of an ultrasound-guided transvaginal approach for oocyte ...
Yap C - - 2000
The physiology of oocyte development, maturation, and selection forms the basis for understanding ovarian follicle dynamics, reproductive function and ovulation induction. The number of ovarian follicles with which women are invested from birth and the rate of decline determine their reproductive lifespan. The processes of atresia and apoptosis constantly deplete ...
- - 2000
This multicentre, double-blind, double-dummy, randomized, parallel-group study compared the efficacy and safety of recombinant human chorionic gonadotrophin (rHCG) (Ovidrel((R))) and urinary HCG (uHCG) (Profasi((R))) for inducing final follicular maturation and early luteinization in women undergoing ovulation induction for assisted reproduction treatment. Following long down-regulation and stimulation with recombinant human FSH ...
Corvo M L - - 2000
PURPOSE: We are exploring liposomal delivery with the aim to change the pharmacokinetics and biodistribution of SOD to increase its therapeutic activity. From a practical point of view, a convenient route of administration would be the subcutaneous (s.c.) route. Liposomal size has been shown to be the most important factor ...
Wennerholm U B - - 2000
The aim of this study was to report the outcome of all clinical pregnancies obtained after intracytoplasmic sperm injection (ICSI) performed during a 5 year period at two fertility clinics, with special reference to delivery outcome associated with different sperm origin and quality and the transfer of fresh or frozen-thawed ...
Dewerchin M - - 2000
Mice with a total deficiency in blood coagulation Factor X (FX) were generated by targeted replacement of an 18-kb fragment of the FX gene, comprising all exons encoding the mature FX protein, with a neo(r) cassette. The genotype distribution among the offspring from heterozygous breeding pairs suggested that FX deficiency ...
Chang S P - - 2000
Poor responders to controlled ovarian hyperstimulation (COH) present a clinical challenge for in vitro fertilization (IVF) and embryo transfer. The failure of IVF for the treatment of severe male-factor infertility can now be overcome by intracytoplasmic sperm injection (ICSI). The infertile couple documented in this case report came to our ...
Seror P - - 1999
OBJECTIVES: The principal aims of this study were to determine the frequency of sepsis after local corticosteroid injection (SALCSI), to compare the results with those of the literature and to determine the main factors leading to a decrease in the frequency of SALCSI. METHODS: A retrospective study was conducted among ...
Gardner R L - - 1999
Although overall polarity is discernable morphologically in both the growing and mature oocyte in mammals, it is typically relatively inconspicuous in the zygote. Furthermore, the conceptus exhibits an essentially radial organization during cleavage which was long held to persist until the primitive streak forms at the onset of gastrulation. This ...
Ménézo Y J - - 1999
OBJECTIVE: To analyze the birth weights and sex ratio of infants born after blastocyst transfer. DESIGN: Retrospective analysis. SETTING: Three infertility clinics. PATIENT(S): Patients admitted for IVF with blastocyst transfer. INTERVENTION: None. MAIN OUTCOME MEASURE(S): Birth weights and sex ratio of infants born after blastocyst transfer. RESULT(S): Statistically significantly more ...
Loft A - - 1999
This national cohort study included all clinical pregnancies obtained after intracytoplasmic sperm injection (ICSI) registered in Denmark between January 1994 and July 1997 at five public and eight private fertility clinics. Laboratory and clinical data were obtained from the fertility clinics. The couples answered a questionnaire regarding the pregnancy and ...
Hinrichs K - - 1999
A nonovulating, hormone-treated mare was used successfully as an oocyte recipient. The mare's ovarian activity was suppressed using progesterone and estrogen treatment. This treatment was stopped, then estrogen was administered for 3 d prior to the transfer. An oocyte was recovered from the follicle of a donor mare and was ...
Keen J E - - 1999
OBJECTIVE: To evaluate the breeding soundness examination procedure in plains bison bulls. DESIGN: Multiyear (1993 through 1997) cross-sectional clinical procedure evaluation. ANIMALS: Two hundred thirty-four 28- to 30-month-old bison bulls at Custer State Park. PROCEDURE: Breeding soundness examinations were performed on all bison bulls using 1992 Society for Theriogenology guidelines ...
Ezeh U I - - 1999
Although testicular biopsy for sperm extraction is a procedure with a potential for complications, sperm retrieval is successful in 30-70% of patients with non-obstructive azoospermia. In order to predict the probability of retrieving at least one testicular spermatozoon we conducted a prospective study of a set of variables in 40 ...
Boone W R - - 1999
In the near future, 6 of 8 bear species will face extinction mainly because of loss of their natural habitat. This loss of habitat will ultimately require some of these bears to be maintained in zoos and wildlife preserves in the hope of conserving genetic diversity. If the giant panda ...
- - 1999
Kim J K - - 1999
This study deals with the morphological degenerations of normal and atretic follicles based on artificially induced radiation apoptosis. ICR strain of 3 week-old female mice were whole body irradiated with 8.3 Gy and sacrificed by cervical dislocation. Ovaries were collected at 0 h, 6 h, 12 h, 1 d, 2 ...
Silva P D - - 1998
OBJECTIVE: To investigate whether gamete intrafallopian transfer (GIFT) may be a cost-effective alternative to donor oocyte procedures in women 40 and older with good ovarian reserve. STUDY DESIGN: Retrospective review of records at an infertility clinic in a large multispecialty group practice. Twenty two consecutive women aged 40 and older ...
Lang I M - - 1998
The objectives of this study were to identify and to characterize the pharyngoesophageal inhibitory reflex (PEIR) in an animal model. Thirty-one cats (2.4-5.0 kg) were anesthetized using alpha-chloralose (45 mg/kg ip), and esophageal peristalsis was recorded manometrically. Secondary peristalsis was activated by rapid air injection (8-20 ml) at midesophagus or ...
Kara A - - 1998
The acute onset of cyanosis in an infant is an alarming development. We report an infant who became cyanotic after injection of prilocaine. Prilocaine is a local anesthetic that is widely used in clinical practice, but usage in infancy easily causes methemoglobinemia. Acquired methemoglobinemia occurs in individuals exposed to an ...
Aytoz A - - 1998
OBJECTIVE: To describe the outcome of pregnancies obtained after intracytoplasmic sperm injection (ICSI) and the impact of the origin and quality of sperm used on this outcome. DESIGN: Retrospective analysis. SETTING: A tertiary referral center for assisted reproduction. PATIENT(S): Pregnant patients conceived after microinjection of ejaculated sperm (n=1,427), epididymal sperm ...
Robert L L - - 1998
The effect of Bacillus sphaericus, at various concentrations, on hatching of phlebotomine sand fly eggs was examined using laboratory bioassays. Aqueous suspensions of B. sphaericus, strain 2362, inhibited hatching of eggs of Phlebotomus duboscqi and Sergentomyia schwetzi by 95% at concentrations as low as 0.05 and 0.11 mg/cm2, respectively. In ...
Quiroga R - - 1998
This study evaluated the performance, acceptability, and appropriateness of a new, single-use, prefilled injection device called UniJect for an outreach immunization application. Between April and June 1995, UniJect devices were used by 36 traditional birth attendants to administer tetanus toxoid injections to 2,240 pregnant women during routine, antenatal home visits ...
Roepstorff A - - 1998
Paired samples of faeces and blood were collected from weaners (W), fatteners (F), lactating sows (S) and piglets (P) in 20 Danish sow herds. The samples were examined by a McMaster technique for Ascaris suum eggs and an indirect ELISA for anti-A. suum IgG. The coprological and serological results were ...
Vidarsson G - - 1998
Streptococcus pneumoniae is a major respiratory pathogen of infants, children, and the elderly. Polysaccharide vaccines have been useful in adult populations but do not elicit protective immunity in infants and young children. To enhance their immunogenicity, vaccines of pneumococcal polysaccharides conjugated to proteins are being developed. In this study antibody ...
Suda T - - 1998
Thirty fertilized chicken eggs preincubated for 0, 7, and 10 days on earth (10 eggs each) were flown in the space shuttle "Endeavour" and further incubated for 7 days under microgravity. Twenty out of 30 eggs (nine out of ten 10-day-old eggs; 10 out of ten 7-day-old eggs; 1 out ...
Aslam I - - 1998
During 1995 and 1996 the first spermatid pregnancies were announced with both round spermatid (ROSI) and elongated spermatid (ELSI) injections. These publications were flanked by live births from ROSI in a number of animal species, with resulting offspring appearing normal, healthy and fertile. However, the live births in humans heralded ...
Cody M M - - 1998
Research is ongoing to develop improved birth control methods and additional contraceptive methods to increase reproductive choices for both men and women. To date, limited male contraceptive choices are available. Immunocontraceptives, injectables, implants, and pills are in developmental stages to become future male contraceptive options. Researchers are pursuing several new ...
Hearn C M - - 1998
The effects of the prolactin inhibiting drug, cabergoline, on pregnant and lactating marsupials were investigated in four species from three diverse families: the tammar wallaby, Macropus eugenii, the quokka, Setonix brachyurus, the brushtail possum, Trichosurus vulpecula, and the fat-tailed dunnart, Sminthopsis crassicaudata. In tammar wallabies, 20 micrograms cabergoline kg-1 injected ...
Latham V H - - 1998
For decades it has been assumed that in order to insert macromolecules into the embryo blastocoel for numerous experimental purposes, microinjection was required. Microinjection, however, can be only performed on a few embryos at a time, thus precluding many studies that could involve large populations of embryos. Laser scanning confocal ...
Lagos R - - 1998
BACKGROUND: In recent years additional parenteral vaccines have been recommended for routine immunization of infants in the US and elsewhere. The ability to administer multiple vaccines as a single injection without unacceptably increasing reactogenicity or decreasing immunogenicity of any component would offer many practical advantages. METHODS: A randomized, open, controlled ...
Songsasen N - - 1998
The objective of this study was to determine the viability and fertility of mouse spermatozoa obtained at various postmortem intervals. Male mice were euthanized, and the bodies were kept at approximately 22 degrees C for up to 24 hr. The epididymides were removed and spermatozoa were allowed to swim out ...
Yang X - - 1998
Since bovine in vitro fertilization became possible in the early 80s, a lot of effort has been done to clarify the mechanisms of what seems more and more one of the crucial steps in this procedure, being oocyte maturation. Undoubtedly, many biological factors act together to prepare the immature oocyte ...
Fanchin R - - 1998
OBJECTIVE: Our purpose was to assess the possible relationship between human embryo growth rates and sexual differentiation. STUDY DESIGN: We analyzed 142 conceptional in vitro fertilization and embryo transfer cycles undertaken in 137 women (151 embryos transferred) in which each embryo transferred led to a gestational sac. Embryos were sorted ...
Zwaan J - - 1997
A 4-month-old infant received an accidental intraocular injection of methylprednisolone while undergoing penetrating keratoplasty for congenital, hereditary endothelial dystrophy. This was not discovered until 4 months later, at which time the intraocular pressure (IOP) was increasing and an early cataract was forming. A lensectomy and vitrectomy were performed when the ...
Kurinczuk J J - - 1997
OBJECTIVE: To test the hypothesis that liveborn infants conceived by intracytoplasmic sperm injection are at an increased risk of having a major birth defect. DESIGN: Reclassification of the birth defects reported in infants born after intracytoplasmic sperm injection in Belgium and comparison with prevalence estimated in Western Australian population by ...
Fox H - - 1997
The clinicopathological features of hydatidiform moles are described. Criteria are given for distinguishing between partial and complete moles and it is stressed that this distinction can be difficult when complete moles are detected, by ultrasound, at an early stage of their development: the diagnostic value of cytometry is emphasized. The ...
Hlywka J J - - 1997
Chicken embryos and brine shrimp naulpii were utilized in short-term toxicity bioassays to assess their sensitivity to the mycotoxin fumonisin B1 (FB1). Fertile chicken eggs (Cobb x) were dosed with FB1 on day 2 of incubation by the injection of 100 microliters of aqueous solution into the air space of ...
Strong C - - 1997
Some have argued that embryos and fetuses have the moral status of personhood because of certain criteria that are satisfied during gestation. However, these attempts to base personhood during gestation on intrinsic characteristics have uniformly been unsuccessful. Within a secular framework, another approach to establishing a moral standing for embryos ...
Porcu E - - 1997
OBJECTIVE: To describe the first birth achieved after intracytoplasmic sperm injection (ICSI) of cryopreserved human oocytes. DESIGN: Case report. SETTING: University of Bologna Hospital, Department of Obstetrics and Gynecology, Reproductive Endocrinology Unit, IVF and Infertility Center. PATIENT(S): One patient undergoing IVF. INTERVENTION(S): Transvaginal ultrasound-guided oocyte retrieval followed by oocyte freezing. ...
Jimenez C - - 1997
OBJECTIVE: To assess whether sperm recovered from postejaculatory urine and cryopreserved can be used successfully for intracytoplasmic sperm injection (ICSI). DESIGN: Case report. SETTING: Laboratory of Developmental and Reproductive Biology, University Hospital. PATIENT(S): A couple with male infertility resulting from complete retrograde ejaculation. INTERVENTION(S): Freezing of sperm recovered from urine ...
Castellotti D S - - 1997
OBJECTIVE: To present a successful transfer of microhatched embryos to the fallopian tubes via microlaparoscopy. DESIGN: Case report. SETTING: Private practice affiliated with a medical university. PATIENT: A 40-year-old woman with primary infertility, mildly elevated baseline FSH levels, and a history of poor ovarian response to ovulation induction. Her husband ...
Meng L - - 1997
Genetically identical nonhuman primates can provide a powerful animal model for gene therapy and research activities where the physiological parameters directly or indirectly under study are heritable. Here we demonstrate that nuclear transfer is a viable technology for the production of identical rhesus macaques. Oocytes recovered from gonadotropin-treated females were ...
Bedford J M - - 1997
The gametes of the least shrew, Cryptotis parva, were studied in regard to their maturation and structure, and with particular emphasis on their behavior in the fallopian tube, from the time of ovulation until the appearance of two-cell embryos beginning some 9 h after ovulation. Cryptotis spermatozoa are organized according ...
Godfrey R W - - 1997
Two trials were conducted to evaluate the efficacy of oestrous synchronisation procedures in St. Croix White, Barbados Blackbelly hair and Florida Native wool ewes. In Trial 1 (conducted in June), 27 ewes were treated with controlled internal drug release (CIDR) devices for 12 days (CIDR1) and 29 untreated ewes served ...
Edirisinghe W R - - 1997
This case report describes the birth of a baby following the transfer of cryopreserved embryos generated from intracytoplasmic sperm injection (ICSI) carried out on the second day after oocyte pick-up of in-vitro-matured metaphase I and germinal vesicle stage oocytes. The couple had a history of three failed intrauterine insemination attempts ...
Soley J T - - 1997
Nuclear condensation during spermiogenesis in the ostrich follows the basic pattern established in other vertebrates. The fine granular nuclear substance of early spermatids is gradually replaced by numbers of coarse dense granules which appear to arise by aggregation of smaller dispersed elements of the chromatin. The granules increase in size ...
Dominko T - - 1997
The effect of maturational state of bovine oocytes at the time of insemination on early embryo development and the sex ratio of developing embryos was evaluated. Early maturing oocytes were inseminated either immediately after the first polar body extrusion or insemination was delayed for 8 h. Most of the zygotes ...
Nurmahmudi - - 1997
In this study, different methods of 0.2 M or 0.3 M CaCl2 delivery into hot-boned spent Leghorn breast fillets followed by tumbling were evaluated. The CaCl2 was delivered by adding 10% (wt/wt) solution into the tumbler, injection 10% (wt/wt) into the fillet, or soaking at room temperature for 15 min ...
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