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Costa E M EM CBQF-Escola Superior de Biotecnologia, Universidade Católica Portuguesa, R. Dr. António Bernardino de Almeida, P-4200-072 Porto, - - 2014
A previous study showed that a chitosan mouthwash would be a valid alternative to current mouthwashes as it demonstrated, in vitro, significantly higher antibiofilm activity than two commercial mouthwashes. As such, the aim of this work was to verify the safety of the developed product and to validate, in vivo, ...
Statland Jeffrey J Department of Neurology, University of Rochester Medical Center, 265 Crittenden Boulevard, CU 420669, Rochester, NY 14642-0669, USA. Electronic address: - - 2014
Facioscapulohumeral muscular dystrophy (FSHD) is a common type of adult muscular dystrophy and is divided into types 1 and 2 based on genetic mutation. Clinically, both FSHD types often show asymmetric and progressive muscle weakness affecting initially the face, shoulder, and arms followed by the distal then proximal lower extremities. ...
Aitken Karen S - - 2014
BackgroundThe understanding of sugarcane genetics has lagged behind that of other members of the Poaceae family such as wheat, rice, barley and sorghum mainly due to the complexity, size and polyploidization of the genome. We have used the genetic map of a sugarcane cultivar to generate a consensus genetic map ...
Kusaba Motoaki M Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Saga, Saga, 840-8502, Japan, - - 2014
A small and extra chromosome of 1.6 Mb was previously identified in a Pyricularia oryzae strain, 84R-62B. To understand a role of the 1.6 Mb chromosome in the pathogenic changeability of P. oryzae, we performed experiments designed to characterize the 1.6 Mb chromosome in the present study. A gene family encoding secreted protein ...
Koubová Martina M Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, Praha 2, Prague, Czech - - 2014
Among amniote vertebrates, geckos represent a clade with exceptional variability in sex determination; however, only a minority of species of this highly diverse group has been studied in this respect. Here, we describe for the first time a female heterogamety in the genus Paroedura, the group radiated in Madagascar and ...
Grabowska-Joachimiak Aleksandra A Department of Plant Breeding and Seed Science, University of Agriculture in Cracow, Łobzowska 24, Cracow, 31-140, Poland, - - 2014
Phleum echinatum Host (2n = 2x = 10) is an annual Mediterranean species which differs from other representatives of the genus Phleum by reduced chromosome number, asymmetric karyotype and unusually high amount of DNA in the genome. Chromosomes of this plant were studied using conventional acetic-orcein staining and fluorescence in situ hybridization (FISH). FISH ...
Towle Rebecca R Department of Integrative Oncology, British Columbia Cancer Research Centre, Vancouver, BC, - - 2014
Genomic alteration at chromosome 9p has been previously reported as a frequent and critical event in oral premalignancy. While this alteration is typically reported as a loss driven by selection for CDKN2A deactivation (at 9p21.3), we detect a recurrent DNA copy number gain of ~2.49 Mbp at chromosome 9p13 in oral ...
Du Xiaoyong - - 2014
The domestic goat (Capra hircus), an important livestock species, belongs to a clade of Ruminantia, Bovidae, together with cattle, buffalo and sheep. The history of genome evolution and chromosomal rearrangements on a small scale in ruminants remain speculative. Recently completed goat genome sequence was released but is still in a ...
Nath Sayantani S Centre of Advanced study, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700 019, West Bengal, - - 2014
The genus Drimia (syn. Urginea), commonly called squill, represents a species complex, infrageneric delimitation being ill-defined due to morphological variability, population variation within species and polyploidy. In the present study, fluorescent chromosome banding and measurements of nuclear DNA content by flow cytometry were performed in five Indian species of Drimia: ...
Garagna Silvia S Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani" and Centro di Ingegneria Tissutale, Dipartimento di Informatica e Sistemistica, Universita' degli Studi di Pavia, Via A. Ferrata, 9, 27100, Pavia, Italy, - - 2014
Many different chromosomal races with reduced chromosome number due to the presence of Robertsonian fusion metacentrics have been described in western Europe and northern Africa, within the distribution area of the western house mouse Mus musculus domesticus. This subspecies of house mouse has become the ideal model for studies to ...
Rajkumar Anto P AP Department of Biomedicine, Institute of Human Genetics, Aarhus University, Aarhus, Denmark; Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark; The Lundbeck Foundation Initiative for Integrative Psychiatric Research, iPSYCH, Aarhus, Denmark; Centre for Integrative Sequencing, iSEQ, Aarhus University, Aarhus, - - 2014
Breakpoints of chromosomal abnormalities facilitate identification of novel candidate genes for psychiatric disorders. Genome-wide significant evidence supports the linkage between chromosome 17q25.3 and bipolar disorder (BD). Co-segregation of translocation t(9;17)(q33.2;q25.3) with psychiatric disorders has been reported. We aimed to narrow down these chromosomal breakpoint regions and to investigate the associations ...
Zheng Fan F *Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China†HKU-Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, - - 2014
Accurate chromosome segregation requires timely bipolar spindle formation during mitosis. The transforming acidic coiled-coil (TACC) family proteins and the ch-TOG family proteins are key players in bipolar spindle formation. They form a complex to stabilize spindle microtubules, mainly dependent of their localization to the centrosome (the spindle pole body/SPB in ...
Vicente Juan-Jesus JJ Department of Molecular & Cell Biology, 345 Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, - - 2014
The binucleate pathogen Giardia intestinalis is a highly divergent eukaryote with a semiopen mitosis, lacking an Anaphase Promoting Complex/Cyclosome (APC/C) and many of the Mitotic Checkpoint Complex (MCC) proteins. However, Giardia has some MCC components (Bub3, Mad2 and Mps1) and proteins from the Cohesin system (Smc1 and Smc3). Mad2 localizes ...
Thévenin Annelyse A Genome Informatics, Faculty of Technology and Institute for Bioinformatics, Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld 33615, Germany IBM Research-Haifa, Mount Carmel, Haifa 3498825, - - 2014
Genomes undergo changes in organization as a result of gene duplications, chromosomal rearrangements and local mutations, among other mechanisms. In contrast to prokaryotes, in which genes of a common function are often organized in operons and reside contiguously along the genome, most eukaryotes show much weaker clustering of genes by ...
Jovicevic Dejana D Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, - - 2014
A team of US researchers recently reported the design, assembly and in vivo functionality of a synthetic chromosome III (SynIII) for the yeast Saccharomyces cerevisiae. The synthetic chromosome was assembled bottom-up from DNA oligomers by teams of students working over several years with researchers as the first part of an ...
Trippe Heike H Department of Neuropediatrics, Developmental Neurology and Social Pediatrics, University of Essen, Essen, - - 2014
Clinically manifesting carriers of Duchenne muscular dystrophy (DMD) are rare among the pediatric population. A standardized diagnostic procedure in supposed DMD carriers entails performing a Multiplex Ligation-dependent Probe Amplification analysis of the DMD gene first, then taking a muscle biopsy to confirm reduced dystrophin levels and/or finally a complete sequencing ...
Ivanauskiene Kristina K University of - - 2014
Histone variant H3.3 is deposited in chromatin at active sites, telomeres and pericentric heterochromatin by distinct chaperone, but the mechanisms of regulation and coordination of chaperone-mediated H3.3 loading remain largely unknown. We show here that the chromatin-associated oncoprotein DEK regulates differential HIRA- and DAAX/ATRX-dependent distribution of H3.3 on chromosomes in ...
Su Albert A *Department of Pathology and Laboratory Medicine, UCLA Medical Center, Los Angeles, CA; and Departments of †Pathology and Laboratory Medicine, and ‡Pediatrics, Division of Hematology/Oncology, Children's Hospital Los Angeles, Keck School of Medicine of University of Southern California, Los Angeles, - - 2014
: Congenital melanoma is extraordinarily rare, and 3 types have been described: transplacental metastases from the mother, de novo congenital melanoma, and melanoma occurring in association with a congenital melanocytic nevus. We describe 2 reports of array comparative genomic hybridization analysis of de novo congenital melanoma. The first patient was ...
Kodama Miyako M University of - - 2014
Whole genome duplication has been implicated in evolutionary innovation and rapid diversification. In salmonid fishes, however, whole genome duplication significantly pre-dates major transitions across the family, and re-diploidization has been a gradual process between genomes that have remained essentially collinear. Nevertheless, pairs of duplicated chromosome arms have diverged at different ...
Berloco Maria M Università degli studi di - - 2014
The dosage effect of Y-chromosome heterochromatin on suppression of position effect variegation (PEV) has long been well-known in Drosophila. The phenotypic effects of increasing the overall dosage of Y heterochromatin have also been demonstrated; hyperploidy of the Y chromosome produces male sterility and many somatic defects including variegation and abnormal ...
Fieremans Nathalie N Human Genome Laboratory, VIB Center for the Biology of Disease, Leuven, - - 2014
Xq28 microduplications of MECP2 are a prominent cause of a severe syndromic form of intellectual disability (ID) in males. Females are usually unaffected through near to complete X-inactivation of the aberrant X chromosome (skewing). In rare cases, affected females have been described due to random X-inactivation. Here, we report on ...
Charron Guillaume G Institut de Biologie Intégrative et des Systèmes, Département de Biologie, PROTEO, Pavillon Charles-Eugène-Marchand, 1030 avenue de la Médecine - Université Laval, Québec, QC, G1V 0A6, - - 2014
Reproductive isolation is a critical step in the process of speciation. Among the most important factors driving reproductive isolation are genetic incompatibilities. Whether these incompatibilities are already present before extrinsic factors prevent gene flow between incipient species remains largely unresolved in natural systems. This question is particularly challenging because it ...
Sahlin Ellika E Department of Molecular Medicine and Surgery and Center for Molecular Medicine, Karolinska Institutet, CMM L8:02, Karolinska University Hospital, Stockholm, - - 2014
Introduction: The underlying causes of stillbirth are heterogeneous and in many cases unexplained. Our aim was to conclude clinical results from karyotype and quantitative fluorescence-polymerase chain reaction (QF-PCR) analysis of all stillbirths occurring in Stockholm County between 2008 and 2012. By screening a subset of cases, we aimed to study ...
Choulet Frédéric F Institut National de la Recherche Agronomique (INRA) UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. University Blaise Pascal, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. - - 2014
We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome ...
Jugulam Mithila M Kansas State University - - 2014
Recent rapid evolution and spread of resistance to the most extensively used herbicide, glyphosate, is a major threat to global crop production. Genetic mechanisms by which weeds evolve resistance to herbicides largely determine the level of resistance, and the rate of evolution resistance. In a previous study, we determined that ...
Levine Michael M Division of Genetics, Genomics, and Development, Center for Integrative Genomics, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: - - 2014
Lucas et al. report the visualization of V(D)J recombination of the immunoglobulin heavy-chain gene (Igh) in living pro-B cells. Despite the huge distances separating V coding sequences from D-J sequences (∼2 Mb), the authors document an astonishingly rapid rate of remote associations. The key to speed is contraction of the Igh ...
L'Abbate Alberto A Department of Biology, University of Bari, Bari, - - 2014
The mechanism for generating double minutes chromosomes (dmin) and homogeneously staining regions (hsr) in cancer is still poorly understood. Through an integrated approach combining next-generation sequencing, single nucleotide polymorphism array, fluorescent in situ hybridization and polymerase chain reaction-based techniques, we inferred the fine structure of MYC-containing dmin/hsr amplicons harboring sequences ...
Vilar Miguel G MG Department of Anthropology, University of Pennsylvania, Philadelphia, PA, - - 2014
Puerto Rico and the surrounding islands rest on the eastern fringe of the Caribbean's Greater Antilles, located less than 100 miles northwest of the Lesser Antilles. Puerto Ricans are genetic descendants of pre-Columbian peoples, as well as peoples of European and African descent through 500 years of migration to the ...
Icsó Diána D Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, - - 2014
The established karyotype was generated by genomic in situ hybridization (GISH) using total barley genomic DNA as labelled probe on mitotic metaphase bread wheat chromosomes. GISH produced specific banding signals on 16 of the 21 chromosome pairs. The following chromosomes showed distinctive banding patterns: 2A, 3A, 4A, 5A, 6A, 7A, ...
Yeung Ks K Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, - - 2014
We report on a baby girl with multiple congenital abnormalities, including cleft palate, intrauterine growth restriction, and double outlet right ventricle (DORV) with ventricular septal defect. She had an unbalanced chromosome translocation t (X;15) resulting in monosomy 15pter → p10 and trisomy Xq13.1 → q28. All three copies of Xq ...
Heim Sverre S Medical Faculty, University of Oslo and Section for Cancer Cytogenetics, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, - - 2014
Microscopic studies of chromosomes in cells cultured from leukaemias and solid tumours have helped confirm the central tenet of Boveri's somatic mutation theory of cancer, namely that acquired chromosomal aberrations of susceptible target cells may cause their neoplastic transformation. Cancer cytogenetics - especially when used together with appropriate molecular genetic ...
Mustafa Ali Moaath K MK Division of Hematology and Oncology, Department of Internal Medicine, King Hussein Cancer Center , Amman , Jordan , - - 2014
Abstract Dasatinib, which is an inhibitor of BCR-ABL and SRC family tyrosine kinases, is used for the treatment of patients with Philadelphia chromosome (Ph) positive leukemia, especially for those who develop resistance or who are intolerant to imatinib. The most common adverse effects attributed to its use are: myelosuppression, nausea, ...
Pérez-García Concepción - - 2014
Mussels of the genus Mytilus present morphologically similar karyotypes that are presumably conserved. The absence of chromosome painting probes in bivalves makes difficult verifying this hypothesis. In this context, we comparatively mapped ribosomal RNA and histone gene families on the chromosomes of Mytilus edulis, M. galloprovincialis, M. trossulus and M. ...
Kim Seon Young SY Seoul National University College of Medicine, Department of Laboratory Medicine, Seoul, Korea, Republic of ; - - 2014
Cytogenetic testing is important to ensure patient safety before therapeutic application of mesenchymal stromal cells (MSCs). However, the standardized methods and criteria for the screening of chromosomal abnormalities of MSCs have not yet been determined. We investigated the frequency of cytogenetic aberrations in MSCs using G-banding and fluorescence in situ ...
Elting Mary Williard MW Department of Cell and Tissue Biology, Biomedical Sciences Graduate Program, and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA - - 2014
The spindle is a dynamic self-assembling machine that coordinates mitosis. The spindle's function depends on its ability to organize microtubules into poles and maintain pole structure despite mechanical challenges and component turnover. Although we know that dynein and NuMA mediate pole formation, our understanding of the forces dynamically maintaining poles ...
Sikirzhytski Vitali V Wadsworth Center, New York State Department of Health, Albany, NY - - 2014
Segregation of genetic material occurs when chromosomes move to opposite spindle poles during mitosis. This movement depends on K-fibers, specialized microtubule (MT) bundles attached to the chromosomes' kinetochores. A long-standing assumption is that continuous K-fibers connect every kinetochore to a spindle pole and the force for chromosome movement is produced ...
Chishti Hafsah Muhammad HM Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan; Institute of Biomedical and Genetic Engineering, Islamabad, - - 2014
Short Tandem Repeat (STR) genetic markers hold great potential in forensic investigations, molecular diagnostics and molecular genetics research. AmpFlSTR® Identifiler™ PCR amplification kit is a multiplex system for co-amplification of 15 STR markers used worldwide in forensic investigations. This study attempts to assess forensic validity of these STRs in Pakistani ...
Tada Sawaki S National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, - - 2014
We truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in Aspergillus oryzae. The predicted deletion was confirmed by Southern blot analyses. This telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in A. oryzae.
Lyozin George T GT Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, - - 2014
Current methods to isolate rare (1:10,000-1:100,000) bacterial artificial chromosome (BAC) recombinants require selectable markers. For seamless BAC mutagenesis, selectable markers need to be removed after isolation of recombinants through counterselection. Here we illustrate founder principle-driven enrichment (FPE), a simple method to rapidly isolate rare recombinants without using selectable markers, allowing ...
Simhadri Srilatha S Rutgers Cancer Institute of New Jersey, United - - 2014
PALB2 links BRCA1 and BRCA2 in homologous recombinational repair of DNA double strand breaks (DSBs). Mono-allelic mutations in PALB2 increase the risk of breast, pancreatic and other cancers, and biallelic mutations cause Fanconi anemia (FA). Like Brca1 and Brca2, systemic knockout of Palb2 in mice results in embryonic lethality. In ...
Makumi Angela A Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, - - 2014
Flp mediated site specific recombination of frt-sites is frequently used in genetic engineering to excise, insert or invert DNA-cassettes in the chromosome. While constructs flanked by frt-sites are generally considered to be stable in the absence of the Flp enzyme, we observed that P22 chromosomes exceeding wild-type length tend to ...
Liu Zhaohui Z Department of Plant Pathology, North Dakota State University, Fargo, ND - - 2014
Barley net form net blotch (NFNB), caused by the necrotrophic fungus Pyrenophora teres f. teres, is a destructive foliar disease in barley-growing regions worldwide. Little is known about the genetic and molecular basis of this pathosystem. Here, we identified a small secreted proteinaceous necrotrophic effector (NE), designated PttNE1 from intercellular ...
Zhao Xiaoli X Department of Hematology, Huashan Hospital, Fudan University, 12 Wulumuqi Road Central, Shanghai, 200040, - - 2014
The mixed lineage leukemia (MLL) gene on chromosome region 11q23 is frequently involved in chromosomal translocations associated with various human hematologic malignant neoplasms. The aim of this study was to investigate the profile of 11q23 abnormalities in adult Chinese patients with hematological malignancies. In this study, 11q23 abnormalities were detected ...
Yamamoto Satoshi S Department of Biochemistry, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke, Tochigi, 329-0498, - - 2014
We developed a transgenic mouse line with Y chromosome-linked green fluorescent protein expressing transgenes (Y-GFP) by the conventional microinjection into the pronucleus of C57BL/6J fertilized oocytes. Embryonic stem (ES) cells derived from Y-GFP mice enabled not only sexing but also the identification of 39, XO karyotype by the lack of ...
Sher Roger B RB University of Maine , Orono, - - 2014
Amyotrophic lateral sclerosis is a late-onset degenerative disease affecting motor neurons in the spinal cord, brainstem, and motor cortex. There is great variation in the expression of ALS symptoms even between siblings who both carry the same Cu/Zn superoxide dismutase (SOD1) mutations. One important use of transgenic mouse models of ...
Guo Mei-Hua MH Kunming Medical University, and Institute of Dermatology & Venereology of Yunnan Province, Kunming, - - 2014
Severe acne presents sexual dimorphism in its incidence in Chinese population. It is more prevalent in males. To assess the possible Y chromosomal contribution to severe acne risk in Han Chinese males, we analyzed 2041 Y chromosomal SNPs (Y-SNPs) in 725 severe acne cases and 651 controls retrieved from our ...
Hamano Tohru T 1Laboratory of Dynamic Cell Biology,Department of Biotechnology,Graduate School of Engineering,Osaka University,Yamadaoka,Suita,Osaka - - 2014
Attempts to elucidate chromosome structure have long remained elusive. Electron microscopy is useful for chromosome structure research because of its high resolution and magnification. However, biological samples such as chromosomes need to be subjected to various preparation steps, including dehydration, drying, and metal/carbon coating, which may induce shrinkage and artifacts. ...
Ferree Patrick M PM W. M. Keck Science Department; Claremont McKenna, Pitzer, and Scripps Colleges; Claremont, CA - - 2014
In eukaryotes, abnormally circularized chromosomes, known as 'rings,' can be mitotically unstable. Some rings derived from a compound X-Y chromosome induce mitotic abnormalities during the embryonic cleavage divisions and early death in Drosophila melanogaster, but the underlying basis is poorly understood. We recently demonstrated that a large region of 359-bp ...
Wang Kexi K University of Toledo, United - - 2014
The mitotic checkpoint (or spindle assembly checkpoint) is a fail-safe mechanism to prevent chromosome missegregation by delaying anaphase onset in the presence of defective kinetochore-microtubule attachment. The target of the checkpoint is the E3 ubiquitin ligase anaphase promoting complex/cyclosome (APC/C). Once all chromosomes are properly attached and bi-oriented at the ...
Takagi Masatoshi M RIKEN, - - 2014
Ki67 is a protein widely used as cell -proliferation marker, with its cellular functions being hardly unveiled. In this paper, we present the direct interaction between Ki67 and PP1γ, a protein phosphatase showing characteristic accumulation on anaphase chromosomes via the canonical PP1-binding motif within Ki67. In cells depleted of Ki67, ...
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