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Results 351 - 400 of 608
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Jamieson G - - 1986
A method of preparing chromosomes from plant root tips for in situ hybridization with tritiated DNA is described. The technique relies on the enzymic hydrolysis of plant cell walls with a pectinase-cellulase mixture. It is shown that, despite the enzymic mixture possessing nuclease activity, there is no detectable degradation of ...
Buys C H - - 1986
Application of the fluorescent DNA-intercalator propidium iodide for stabilization of the mitotic chromosome structure during isolation of chromosomes from V79 Chinese hamster cells and subsequent staining with the fluorochromes 33258 Hoechst or DAPI allowed bivariate flow karyotyping of isolated chromosomes. Fluorescence of 33258 Hoechst bound to isolated chromosomes containing 5-bromodeoxyuridine ...
Perry P E - - 1986
A method is presented for the phenotypic identification of metaphase cells stained for chromosome aberration and SCE analysis. The cells are labeled in suspension with antibodies conjugated with colloidal gold, and then chromosome preparations are made using a cytocentrifuge. A silver development (IGSS) procedure is used to enhance the gold ...
Takayama S - - 1986
Chinese hamster cells were grown with 50 microM 5-bromodeoxyuridine (BrdU) during the penultimate S phase to obtain chromosomes with the TB-TT chromatid constitution. Chromosome preparations made by the airdrying method were used to study the sister chromatid differential staining (SCD) resulting from ultraviolet (UV) irradiation followed by Giemsa staining by ...
Ross M D - - 1985
Earlier students of the origin of Lotus corniculatus suggested that this tetraploid species arose as an autotetraploid of the closely related diploid species L. tenuis or L. alpinus. More recent studies suggested that L. alpinus and L. japonicus could be ancestral forms. The present study of tannin content, phenolic content, ...
Lee G M - - 1985
Various pretreatments of metaphase spreads were examined to obtain optimal DNA labelling patterns while maintaining chromosome integrity during in situ hybridization procedures. Preparations of African green monkey (AGM) chromosomes fixed in methanol-acetic acid (CV-1 cell line) were treated by coating with Denhardt's solution, dilute gelatin-chrome alum, nonfat instant dry milk ...
Martin A O - - 1985
Silver staining of human chromosomes at prometaphase or metaphase identifies variants in the stalk (nucleolar organizing) regions of acrocentric chromosomes (Nos. 13, 14, 15, 21, 22). Variants are defined by size, number, and morphology of silver staining areas. They are heritable polymorphisms and have not been associated with clinical abnormalities. ...
Thomsen J L - - 1985
The effect of various fixatives on Y-chromosome detection in blood smears was examined. Fixatives containing alcohol or acetic acid seemed to be more suitable. Formaldehyde, Bouin's fluid and CrO3 gave poor results. Various storage conditions were examined. When comparing fixation in Carnoy's solution. Clarke's solution and Metha-Clarke the Y-body index ...
Kindiger B - - 1985
Studies of postmeiotic chromosome behavior have been impeded by the thick exine and abundant starch grains of maize pollen. Staining pollen grain chromosomes with acetocarmine is tedious and gives inconsistent, often unsatisfactory results. A hematoxylin stain, used in conjunction with the clearing agent chloral hydrate, has been successfully used by ...
Motara M A - - 1985
Application of the silver-staining technique to air-dried chromosome preparations of the yellow fever mosquito, Aedes aegypti, revealed the following: 1) intensely stained pericentromeric regions in all chromosomes including the Y chromosome in spermatogonia, and during the primary and secondary spermatocytes; 2) the presence of prepachytene that were not reported earlier; ...
Tommerup N - - 1985
Combined staining of non-cultured interphase nuclei from hydatidiform moles with distamycin A and DAPI resulted in the presence of specific stained interphase bodies. A mean number of six interphase bodies was observed in moles with a diploid karyotype and a mean number of nine interphase bodies was observed in triploid ...
Jan K Y - - 1985
Opposite differential staining between sister chromatids was obtained by two silver-staining techniques on chromosomes replicated twice in medium containing 5-bromodeoxyuridine (BrdU) and pretreated with Hoechst plus black light. Both silver-nitrate and silver-carbonate staining were affected by chemical extraction and enzyme digestion of chromosomal proteins. Prestaining of silver nitrate or silver ...
Romagnano A - - 1985
A technique is described for the preparation of banded chromosomes from early equine embryos cultured for less than 10 h in a medium containing bromodeoxyuridine. In addition to standard Giemsa staining and C-banding, chromosomes thus prepared can also be R-banded by either the RBA or the RB-FPG methods. This technique ...
Amenomori T - - 1985
We report a micromethod for chromosome preparation from individual hematopoietic colonies cultured in methylcellulose. The entire process was carried out on poly-Lysine (PL)-coated slides. Individual colonies were transferred into 10 microliter of 0.075 M KCl and placed on PL-coated slides. After hypotonic treatment of the colony cells and their attachment ...
Takayama S - - 1985
Vero cells (African green monkey kidney in origin) were prepared by the conventional air-drying method and then processed for SEM by a modification of the conductive method based on thiocarbohydrazide-osmium binding. Under SEM, not only metaphase chromosomes but also resting nuclei showed distinct fibres 30 nm in diameter. A few ...
Jan K Y - - 1985
A differential Giemsa staining between sister chromatids was obtained by treating chromosomes replicated twice in medium containing 5-bromodeoxyuridine (BrdU) with Hoechst 33258 plus black light at 55 degrees C (HB pretreatment) and deoxyribonuclease (DNase) I, II, or micrococcal nuclease. In this staining pattern the BrdU bifilarly substituted chromatids were darkly ...
Almássy Z - - 1985
Human lymphocytes were cultured for two cell cycles in the presence of bromodeoxyuridine (BrdU), and the resulting metaphase chromosomes were first stained with quinacrine mustard (QM) and then, immediately afterwards, with Hoechst 33258, without any intermediate destaining. Both Q-banding patterns and sister chromatid differential staining were photographed subsequently on the ...
van den Engh G J - - 1985
Chromosomes were isolated from a variety of human cell types using a HEPES-buffered hypotonic solution (pH 8.0) containing KCl, MgSO4, dithioerythritol, and RNase. The chromosomes isolated by this procedure could be stained with a variety of fluorescent stains including propidium iodide, chromomycin A3, and Hoechst 33258. Addition of sodium citrate ...
van Duijn P - - 1985
A new hypothesis is proposed on the involvement of nucleosomes in Giemsa banding of chromosomes. Giemsa staining as well as the concomitant swelling can be explained as an insertion of the triple charged hydrophobic dye complex between the negatively-charged super-coiled helical DNA and the denatured histone cores of the nucleosomes ...
Otto F - - 1985
A comparison of four DNA stains considered to be AT-specific with chromosomes from a clonal Chinese hamster cell line B14F28-C5 have been made. The flow karyotype histograms indicate that DAPI, DIPI, and Hoechst 33258 and 33342 do stain similarly in the same preparation. DAPI staining is specific and highly reproducible ...
Lalande M - - 1985
A dual laser FACS IV cell sorter has been used to obtain bivariate flow histograms of human metaphase chromosomes stained with the DNA-specific dyes, 33258 Hoechst and chromomycin A3. Approximately twenty distinct chromosomal fluorescence populations can be resolved using this double staining technique and the flow cytometer which has been ...
Kroisel P M - - 1985
The dye triplet chromomycin/methyl green/DAPI and some related dye combinations were applied to bromodeoxyuridine (BrdU)-substituted and non-substituted chromosomes. In both kinds of chromosome preparations, tri-staining followed by observation at pH 7 resulted in well defined R-bands (excitation wavelength 436 nm) and a mixed DA-DAPI/Q-banding pattern (360 nm). Two approaches have ...
Zenker H J - - 1985
Two types of cyclitis posterior had to be differentiated owing to the fluorescein angiographic pattern observed. At first, a diffuse, belt-shaped staining of the whole pars plana, and secondly, a circumscribed staining of the inflammatory foci. Both types were combined with the permeation of dye into the cystoid spaces as ...
Pukkila P J - - 1985
We have taken advantage of the synchronous meiotic process in the basidiomycete Coprinus cinereus to develop a simple and rapid method to selectively stain meiotic chromosomes and nucleoli in this fungus without prior removal of the cell wall. Electron microscopic examination of these silver-stained chromosomes indicated that the lateral elements ...
Tonew M S - - 1985
Chick embryo cell monolayers (CEC) treated by acidified thiazine dyes (pH 5 to 3) arrived into a mitogenic state. The diffuse dispersed chromatin of the nuclei clustered on the nuclear membranes and on the nucleoli in chromosome-like figures is usually estimated by electron microscopy. The clustering process could better be ...
Holden J J - - 1985
Homogeneously staining regions (HSRs) in the human melanoma cell line, MeWo, are located on an X and a der(15) chromosome. These regions are homogeneously stained with quinacrine fluorescence, but stain differentially with conventional Giemsa, G-banding, C-banding, and distamycin A/4',6-diamidino-2-phenylindole. There are five and six blocks of positively staining material on ...
Meyne J - - 1984
Isolated human metaphase chromosomes stained with the fluorochromes 4'-6-diamidino-2-phenylindole (DAPI) and chromomycin A3(CA3), and counterstained with nonfluorescent netropsin (NTR), have been analyzed by dual-laser flow cytometry. Counterstaining with NTR reduces DAPI fluorescence except at regions on chromosomes 1,9,15,16, and Y, corresponding to C-band heterochromatin. Bivariate flow karyology of human chromosomes ...
Mudd J L - - 1984
The determination of sex from forcibly removed hairs in forensic science laboratories has, in the past, been based almost entirely on the presence or absence of the Y chromosome in the cells of the hair root sheath. Since the human male genotype is XY and the female is XX, a ...
Paoletti L - - 1984
Talc powders from national and international markets were analyzed in order to assess their fiber contents and the proportion of asbestos in the fibrous material. Samples of talc powders used as excipients in pharmaceutical and cosmetic preparations demonstrated fiber contents up to 30% of total particles. About a half of ...
Antohi S - - 1984
As evaluated by light scattering at 90 degrees, natural organic oligocations such as putrescine, spermidine and spermine interfered with myxovirus aggregates which were induced by the histone H2A and strongly amplified by shaking during incubation. In contrast, the synthetic oligocation 1.7-diamino heptane itself aggregated the virus particles, its action being ...
Tuck-Muller C M - - 1984
A reliable technique for combined C-banding and silver staining of metaphase chromosomes which uses trypsinization is described. Slides are first immersed in dilute HCl to remove residual cytoplasm from around the chromosomes. They are then treated with saturated barium hydroxide and incubated overnight in saline sodium citrate (0.30 M NaCl, ...
Mayall B H - - 1984
Image cytometry and computer analysis are used to determine the relative DNA content and the DNA-based centromeric index of the 24 chromosomes of the human karyotype. A two-step procedure is used. Chromosomes of cells in metaphase first are stained with quinacrine and identified visually by their fluorescent Q-band patterns. They ...
Simmons M C - - 1984
The human melanoma cell line MeWo was found to contain two populations of cells, one containing 83 chromosomes (hypotetraploid) and the other containing 43 chromosomes (hypodiploid). All of the hypodiploid cells, but none of the hypotetraploid cells, contained chromosomes with long homogeneously staining regions (HSR's) when examined with quinacrine fluorescence. ...
van den Engh G - - 1984
Certain variables in the preparation of chromosome suspensions for flow cytometric analysis have been investigated. The optimal conditions have been determined. The results of this series of experiments have been incorporated to yield a preparation protocol that gives chromosome profiles with a low amount of small particle debris and few ...
SkawiƄski W - - 1984
The length of the Y chromosome with its fluorescent and non-fluorescent portion was measured in Q-banded metaphases. The same preparations were stained by Feulgen method. The DNA content and size of the Y chromosome were assessed. The length of Y chromosome was normalized by taking into account differences in contraction ...
Mayr B - - 1984
The karyotype of the European wild pig (Sus scrofa scrofa L.) was analysed by means of silver-staining and the chromomycin A3/distamycin A/DAPI fluorescent banding technique to locate active NORs and to differentiate types of C-bands. The ribosomal RNA genes are localized at the secondary constrictions of chromosomes 8 and 10. ...
Traut W - - 1984
An extra segment in chromosome 1 between bands C5 and D has been found in wild mouse populations. Its size varies between 6.1% and 30.1% of the length of a standard chromosome 1. It differs among individuals and populations but is constant in a genetically homogeneous line. According to its ...
Green D K - - 1984
Frequency distributions of fluorescence intensity of ethidium bromide stained human chromosomes from nine phenotypically normal males are cross correlated and autocorrelated following repeated flow cytometric measurements. It is shown that each individual donor produces a fluorescence profile which is both visually and numerically different from those of other individuals in ...
Verma R S - - 1984
The expression of ribosomal cistrons in the nucleolar organizer regions (NORs) has been studied with high resolution banding in the acrocentric chromosomes of 10 normal individuals. It was found that if a particular chromosome did not stain with silver nitrate at metaphase, then it did not stain at prophase either. ...
Fernandez-Piqueras J - - 1984
A simple method is presented by which synaptonemal complexes (SCs) can be visualized under bright field microscopy. Staining was accomplished with a silver nitrate solution adjusted to pH 3-3.5 with formic acid, after an acidic fixation. This method is suitable for the demonstration of SCs, centromeres and NOR regions in ...
Earnshaw W C - - 1984
Cytological silver-staining procedures reveal the presence of a "core" running along the chromatid axes of isolated HeLa mitotic chromosomes. In this communication we examine the relationship between this "core" and the nonhistone chromosome scaffolding, isolated and characterized in previous publications from this laboratory. When chromosomes on coverslips were subjected to ...
Lavezzi A M - - 1984
Cultures of bone marrow obtained from patients with various forms of leukemia have been stained by the Giemsa-11 technique. The results suggest that this method can be utilized for the constant monitoring of numerical and structural anomalies in chromosome 9. Furthermore, by varying the pH, this technique can be applied ...
Young B D - - 1984
The latest developments in the application of flow cytometry to the analysis and fractionation of metaphase chromosomes is reviewed. This includes the preparative techniques, staining and machine technology used in flow karyotype production. In particular, the application of this technology to the study of chromosomal changes and to the construction ...
Buys C H - - 1984
Oxidizing Methylene Blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin. This staining can be combined in a sequential procedure with a fluorescent banding technique allowing the exact ...
Lezhava T A - - 1984
Ag-positive nucleolar organizer regions (NORs) and associations of silver-stained acrocentric chromosomes were studied in 400 metaphases from 20 male and female subjects aged 20-50 and 80 or over. The number of Ag-positive NORs and the frequency of chromosome associations were found to be decreased significantly at 80 and thereafter in ...
Tuck-Muller C M - - 1984
Associations between nucleolus organizer regions (NORs) and non-acrocentric chromosomes were scored in 2,800 metaphase spreads from PHA-stimulated lymphocyte cultures (48 h) from 14 individuals. The preparations were both silver stained and C-banded. In order to calculate the expected values for associations, the ratio of heterochromatin length to euchromatin length was ...
Viegas-Pequignot E - - 1984
A large amount of heterochromatin is observed in two species of the genus Gerbillus, G. nigeriae and G. hesperinus. The C-band material represents about one-half of the total karyotype length in the former species, and about one-third in the latter. Several banding techniques and various 5-bromodeoxyuridine (BrdU) treatments were used ...
Coyle P K - - 1983
We applied a sensitive silver nitrate stain to SDS-polyacrylamide gels to detect oligoclonal bands in unconcentrated CSF. Silver staining took less than 3 hours and gave easily readable bands in 93% of MS patients. In contrast, standard Coomassie Blue stain was positive in only 53%. Maximum resolution and numbers of ...
Loidl J - - 1983
Microsporogenesis of Allium flavum was investigated by light microscopy using a silver impregnation technique. Ag-positive structures were present at the nucleolus organizing regions (NORs) in all stages of the meiotic cycle. During prophase I the nucleoli were found to be composed of a strongly impregnated central and a weakly impregnated ...
Duan X S - - 1983
The technique of cell fusion, the method of sister chromatid differentiation (SCD), and the silver staining method modified by us that can show nucleolus organizing region (NOR) and sister chromatid differentiation at the same time have been used in the study of red muntjac chromosomes. Differential staining of sister chromatid ...
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