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Results 301 - 350 of 594
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Weiss J S - - 1988
To determine the diagnostic usefulness of routine staining of corneal buttons with alcian blue, we evaluated 150 consecutive corneal specimens with hematoxylin and eosin and alcian blue stains. Overall, 67% of specimens demonstrated deposition of acid mucopolysaccharide. More specifically, positive alcian blue staining was observed in 88% of specimens with ...
Dhaliwal M K - - 1988
Silver staining of cells in metaphase and interphase nuclei of both sexes of the Bennett wallaby, Macropus rufogriseus, has shown that (1) the nucleolus organizer region (NOR) is located only on the X chromosome (single Ag-NOR); (2) both X chromosomes in the female cells stain with silver; (3) the amounts ...
Gutiérrez-Gonzálvez M G - - 1988
We studied the effectiveness of harmine as a substitute for 33258 Hoechst in the fluorescence-plus-Giemsa technique, using Allium cepa chromosomes after 5-bromo-2'-deoxyuridine (BrdU) incorporation. Harmine showed a photosensitizing capacity which was somewhat higher than 33258 Hoechst and used half of the time established for the usual treatment.
Bowling A T - - 1988
A fourth allele at the horse erythrocyte phosphohexose isomerase (Phi) locus was proposed to account for phenotypes observed after starch gel electrophoresis and enzymatic staining of red cell lysates from American Saddlebred and Tennessee Walking Horse breeds. The gene was rare, having an estimated frequency of 0.009 in 949 Saddlebreds ...
Cerdán E - - 1988
Denaturation and posterior renaturation of metaphase chromosomes after different treatments were tested by acridine orange staining. A relationship between conservation of ultrastructure and the capacity to renature was discovered. Amyl acetate, commonly used in electron microscopy to fix chromosomes, avoids the action of denaturing agents and this effect is not ...
Kopp E - - 1988
The expression of nucleolus organizer region (NOR) activity in diploid cells was investigated in a model mammalian hybrid system, the hinny (female ass x male horse), by sequential Ag-NOR and chromomycin A3/distamycin A/DAPI (CDD) staining ion lectin-stimulated peripheral blood lymphocytes. In the majority of cases we found non-expression of the ...
Mayr B - - 1987
Ag-NOR staining and a counterstain-enhanced fluorescence technique (chromomycin A3/distamycin A/DAPI staining = CDD-method) have been applied to ibex (Capra ibex L.), chamois (Rupicapra rupicapra L.) and bison (Bison bison L.) chromosomes. Chromomycin A3 visualization led to a well defined R-banding pattern along the chromosome arms and to a clear demonstration ...
Pittman S - - 1987
A method is described for visualising chromosome-mediated gene transfer (CMGT) by detecting chromosomes labelled with bromodeoxyuridine (BrdU) using a monoclonal antibody to BrdU. In this experiment, the CCRF-CEM T cell line was grown in the presence of BrdU and the labelled chromosomes were isolated and transfected into human embryonic fibroblasts. ...
Conia J - - 1987
A method to obtain a high metaphase index and thereafter a plant chromosome suspension is described for Petunia hybrida (2n = 14). Mesophyll protoplast cultures have been used, giving easily disrupted cell walls and a high percentage of dividing cells after 42 h. On 2.5 mM colchicine-treated cells, metaphase indexes ...
Cherry L M - - 1987
Antikinetochore immunofluorescence staining has been used in several studies to determine whether a second kinetochore is present, active, or both, in multicentric chromosomes. All of these studies have used tissue culture cells, and contended with the problem of obtaining well spread, banded metaphase chromosomes without affecting the kinetochore staining. We ...
Chen R - - 1987
The efficiency of each of two methods for monitoring the occurrence of a rare event, the cusum, carried out monthly, and the sets techniques, was assessed by use of simulated datasets. The monthly cusum parameters used in this study were those recommended for monitoring of birth defects in a monthly ...
Jongedijk E - - 1987
A simple enzyme squash technique that enables detailed studies of meiosis in potato ovules has been developed. Fixation of ovules in iron-propionic-ethanol followed by enzymatic maceration and squashing in acetocarmine yielded numerous well preserved megasporocytes with nicely spread chromosomes. Resolution was sufficient, allowing detailed analysis of chromosome pairing and chiasma ...
Fredga K - - 1987
Chromosome preparations of high quality can be obtained from bone marrow cells of small mammals that have been dead for 20 hr or longer. The bone marrow is rinsed out of the femurs with RPMI medium supplemented with 15% fetal calf serum. Add 0.05-0.1 ml of a 0.01% colchicine solution ...
Wurster-Hill D H - - 1987
The G-banded karyotype of the Iriomote cat, Felis iriomotensis, was found to be indistinguishable from that of F. bengalensis, F. viverrinus, and F. concolor. The karyotype of these four cats is, however, distinguishable from karyotypes of all other species of cats whose chromosomes have been studied. Silver-staining showed nucleolar organizing ...
Lomholt B E - - 1987
The effect of pH on silver staining of the nucleolus organizer regions (NORs) of human chromosomes has been investigated between pH 6.5 and 12.0. Nonvolatile mixtures of ethanolamine and ethanolammonium nitrate replaced the ammonia of standard procedures. The optimal NOR staining obtained at pH 3.5 by the silver staining procedure ...
Lebo R V - - 1987
A high-resolution triple-laser sorter was designed and constructed to provide flexible switchover and high-resolution sorting of cells or chromosomes with any combination of one, two, or three lasers. These features provide a central facility instrument that currently serves multiple users and analyzes different stain combinations with minimal switchover effort between ...
Weier H U - - 1987
A data acquisition system is described for recording two independent signals simultaneously from a laser-based flow cytometer for rapid slit-scan chromosome analysis. High-aperture microscope optics allow recording of fluorescence distributions along the longest axis of metaphase chromosomes with a spatial resolution better than 1 micron. Fluorescence and small angle forward ...
Aten J A - - 1987
A number of structurally unrelated DNA intercalators have been studied as stabilizers of mitotic chromosomes during isolation from rodent and human metaphase cells. Seven out of the nine intercalators tested were found to be useful as chromosome stabilizing agents. Chromosome suspensions prepared in this way could be preserved for long ...
Ploton D - - 1987
The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis in five human and murine cancerous cell lines each characterized by a typical nucleolar morphology. During interphase the Ag-NOR proteins ...
Lebo R V - - 1986
Our custom dual laser chromosome sorter has been used to characterize and isolate metaphase human chromosomes rapidly for gene mapping purposes. Herein, we tested how well this system could detect unknown abnormal human chromosome constitutions. These results were compared to those of conventional cytogenetic analyses by banding and photomicrography. The ...
Mayr B - - 1986
Sequential staining with a counterstain-contrasted fluorescent banding technique (chromomycin A3-distamycin A-DAPI) revealed the occurrence of distamycin A-4,6-diamidino-2-phenylindole (DA-DAPI) staining heterochromatin in the centromeric regions of chromosomes 33, 36, 37, and 38 in the wolf (Canis lupus pallipes) and of chromosomes 13, 16, and 23 in the blue fox (Alopex lagopus). ...
Babu A - - 1986
We suggest that the short arms of human chromosome 15 (15p) exhibit intensity heteromorphisms by DA/DAPI technique. A method for classification of variable intensities is proposed. The different intensities can be classified into at least five classes. They are: negative, pale, medium, intense, and brilliant. Therefore we suggest that 15p ...
Dietrich A J - - 1986
It is well known that there is a strong influence of fixation, i.e., acetic methanol versus formaldehyde, on the chromosome morphology at stages of the first meiotic division. In this study the influence of both these types of fixation on the morphology of mitotic chromosomes was examined in human lymphocytes. ...
Finnon P - - 1986
The Cytoscan 110 metaphase finder has been tested with cultures of human peripheral blood lymphocytes prior to its introduction into routine use for the analysis of radiation-induced chromosomal damage. Cells of varying quality and density of distribution on slides, stained with orcein or Giemsa, were examined by the same technician ...
Babu A - - 1986
The staining property of pericentromeric heterochromatin of chromosome 18 is compared by C-banding and restriction endonuclease AluI digestion methods. Only a small distal fraction of C-band of chromosome 18 is observed to be resistant to AluI treatment, which positively stained with subsequent Giemsa staining. The resistant fraction is characteristic and ...
Verma R S - - 1986
A possible cause of non-disjunction of chromosome 21 in Down Syndromes has been cytogenetically evaluated by examining the parents by Ag-staining technique. In all the cases studied so far, the contributing parents have active ribosomal cistrons on both chromosomes 21 i.e. both chromosomes are stained positively by silver staining. These ...
Vogel W - - 1986
We have shown that a commercially available antibody allows the demonstration of bromodeoxyuridine (BrdU) incorporation into mammalian chromosomes by the typical immunologic staining techniques. The replication patterns obtained are comparable to those after BrdU-incorporation and FPG-staining, though with reversed staining specificity. The quality of the patterns equals that of commonly ...
Kovacs G - - 1986
Cytogenetical analysis of bone marrow and peripheral blood cells in a case of erythroleukaemia revealed a complex karyotype with a stemline of 44 chromosomes. 1 marker chromosome bearing a homogeneously staining region (hsr) was found in each of the aneuploid cells examined. The hsr was localised to the chromosome regions ...
Nielsen P B - - 1986
An assessment of the Gram, the Methylene blue, and the Ziehl-Neelsen procedures in diagnosing Pneumocystis carinii has been performed. By using heat and methanol as fixatives and phase-contrast microscopy we found the procedures valuable in detecting the pneumocysts, especially in the Gram staining. The diagnosis should, however, be confirmed by ...
Kopp E - - 1986
The expression of nucleolus organizer activity in diploid cells was investigated in a model system for mammalian hybrids, the horse-ass cross (mule), by means of sequential Ag-NOR and chromomycin A3/distamycin A-DAPI (CDD) staining in lectin-stimulated peripheral blood lymphocytes (PBL). As a rule we found species-specific non-expression of the horse-derived NOR ...
Babu A - - 1986
The constitutive heterochromatin of the Indian muntjac (Muntiacus muntjak) was examined following digestion with various restriction endonucleases (AluI, HaeIII, HinfI, and MboI), as well as by selective fluorescence staining with distamycin A plus 4'-6-diamidino-2-phenylindole. Distinct areas within the C-bands were found to have characteristic staining patterns which were more conspicuous ...
Vanni R - - 1986
Sequential staining with Giemsa and quinacrine mustard on direct cytogenetic preparations was employed to characterize the karyotype of five bladder tumors of different stage and grade (TNM classification). The use of QFQ-banding allowed: the characterization of 2 euploid and 1 pseudodiploid cases, and the identification, in the two hyperdiploid cases, ...
Ferrucci L - - 1986
DA-DAPI counterstaining is a technique which reveals the heterochromatic areas of chromosomes 1, 9, 15, 16 and Y in humans; a result which may be related to the peculiar characteristics of these chromosomal regions, particularly regarding their DNA content. The present report describes a different DA-DAPI stain reaction. Obtained under ...
Kido A - - 1986
The polymorphism of alpha-L-fucosidase (Fu) was investigated in a Japanese population from samples of placentae and semen, using isoelectric focusing. The gene frequencies of placental types were Fu1 = 0.748 and Fu2 = 0.252, and those of seminal types were Fu1 = 0.739 and Fu2 = 0.261. The coincidence in ...
van den Engh G J - - 1986
The interactions and binding characteristics of DNA dyes used in the flow cytometric analysis of chromatin were studied using human chromosomes and mouse thymocyte nuclei. The kinetics of dye binding and the relationship between fluorescence intensity and dye concentration are presented. Under the conditions used, Hoechst 33258, propidium iodide and ...
Schmid M - - 1986
The chromosomes of the gorilla were extensively studied with various staining techniques labeling the different classes of heterochromatin. The chromosomal distribution of distamycin A/DAPI-, D287/170-, quinacrine-, and chromomycin A3-positive heterochromatic regions, as well as the nucleolus organizer regions, is described and compared with the karyotypes of other hominoid species. Lymphocyte ...
Jamieson G - - 1986
A method of preparing chromosomes from plant root tips for in situ hybridization with tritiated DNA is described. The technique relies on the enzymic hydrolysis of plant cell walls with a pectinase-cellulase mixture. It is shown that, despite the enzymic mixture possessing nuclease activity, there is no detectable degradation of ...
Buys C H - - 1986
Application of the fluorescent DNA-intercalator propidium iodide for stabilization of the mitotic chromosome structure during isolation of chromosomes from V79 Chinese hamster cells and subsequent staining with the fluorochromes 33258 Hoechst or DAPI allowed bivariate flow karyotyping of isolated chromosomes. Fluorescence of 33258 Hoechst bound to isolated chromosomes containing 5-bromodeoxyuridine ...
Perry P E - - 1986
A method is presented for the phenotypic identification of metaphase cells stained for chromosome aberration and SCE analysis. The cells are labeled in suspension with antibodies conjugated with colloidal gold, and then chromosome preparations are made using a cytocentrifuge. A silver development (IGSS) procedure is used to enhance the gold ...
Takayama S - - 1986
Chinese hamster cells were grown with 50 microM 5-bromodeoxyuridine (BrdU) during the penultimate S phase to obtain chromosomes with the TB-TT chromatid constitution. Chromosome preparations made by the airdrying method were used to study the sister chromatid differential staining (SCD) resulting from ultraviolet (UV) irradiation followed by Giemsa staining by ...
Ross M D - - 1985
Earlier students of the origin of Lotus corniculatus suggested that this tetraploid species arose as an autotetraploid of the closely related diploid species L. tenuis or L. alpinus. More recent studies suggested that L. alpinus and L. japonicus could be ancestral forms. The present study of tannin content, phenolic content, ...
Lee G M - - 1985
Various pretreatments of metaphase spreads were examined to obtain optimal DNA labelling patterns while maintaining chromosome integrity during in situ hybridization procedures. Preparations of African green monkey (AGM) chromosomes fixed in methanol-acetic acid (CV-1 cell line) were treated by coating with Denhardt's solution, dilute gelatin-chrome alum, nonfat instant dry milk ...
Martin A O - - 1985
Silver staining of human chromosomes at prometaphase or metaphase identifies variants in the stalk (nucleolar organizing) regions of acrocentric chromosomes (Nos. 13, 14, 15, 21, 22). Variants are defined by size, number, and morphology of silver staining areas. They are heritable polymorphisms and have not been associated with clinical abnormalities. ...
Thomsen J L - - 1985
The effect of various fixatives on Y-chromosome detection in blood smears was examined. Fixatives containing alcohol or acetic acid seemed to be more suitable. Formaldehyde, Bouin's fluid and CrO3 gave poor results. Various storage conditions were examined. When comparing fixation in Carnoy's solution. Clarke's solution and Metha-Clarke the Y-body index ...
Kindiger B - - 1985
Studies of postmeiotic chromosome behavior have been impeded by the thick exine and abundant starch grains of maize pollen. Staining pollen grain chromosomes with acetocarmine is tedious and gives inconsistent, often unsatisfactory results. A hematoxylin stain, used in conjunction with the clearing agent chloral hydrate, has been successfully used by ...
Motara M A - - 1985
Application of the silver-staining technique to air-dried chromosome preparations of the yellow fever mosquito, Aedes aegypti, revealed the following: 1) intensely stained pericentromeric regions in all chromosomes including the Y chromosome in spermatogonia, and during the primary and secondary spermatocytes; 2) the presence of prepachytene that were not reported earlier; ...
Tommerup N - - 1985
Combined staining of non-cultured interphase nuclei from hydatidiform moles with distamycin A and DAPI resulted in the presence of specific stained interphase bodies. A mean number of six interphase bodies was observed in moles with a diploid karyotype and a mean number of nine interphase bodies was observed in triploid ...
Jan K Y - - 1985
Opposite differential staining between sister chromatids was obtained by two silver-staining techniques on chromosomes replicated twice in medium containing 5-bromodeoxyuridine (BrdU) and pretreated with Hoechst plus black light. Both silver-nitrate and silver-carbonate staining were affected by chemical extraction and enzyme digestion of chromosomal proteins. Prestaining of silver nitrate or silver ...
Romagnano A - - 1985
A technique is described for the preparation of banded chromosomes from early equine embryos cultured for less than 10 h in a medium containing bromodeoxyuridine. In addition to standard Giemsa staining and C-banding, chromosomes thus prepared can also be R-banded by either the RBA or the RB-FPG methods. This technique ...
Amenomori T - - 1985
We report a micromethod for chromosome preparation from individual hematopoietic colonies cultured in methylcellulose. The entire process was carried out on poly-Lysine (PL)-coated slides. Individual colonies were transferred into 10 microliter of 0.075 M KCl and placed on PL-coated slides. After hypotonic treatment of the colony cells and their attachment ...
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