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Sentein P - - 1976
A combination of methods for fixation (sublimate, cobalt nitrate, formaldehyde, acetic acid in water), inclusion (celloidin dissolved in methyl salicylate, paraffin-paraplast) and staining was used to make serial sections easy, to avoid clefts and to give a good picture of segmentation mitoses, as well as a good contrast of yolk ...
Goodpasture C - - 1976
A silver-staining technique specific for demonstrating nucleolus organizer regions (NOR) showed that the achromatic stalks of the 10 acrocentric autosomes of the human complement represent the NORs. Some variability in number of stained stalks is observed from cell-to-cell and from individual-to-individual. The silver-stained masses may extend beyond the stalks and ...
Bloom S E - - 1976
A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal identification of chromosome pairs bearing NOR's can be achieved ...
Jordan E G - - 1976
Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L ...
Schwarzacher H G - - 1976
Metaphase chromosomes of mouse strain L cells show strikingly uncondensed pericentric heterochromatic regions after treatment of living cells with the benzimidazol-derivative 33 258 Hoechst. In electron micrographs of total preparations after G-band staining the chromosomes are seen to be made up of irregularly folded fibrils of 200-400 A in diameter. ...
Howell W M - - 1976
Human metaphase chromosomes were stained with silver following a pretreatment with a heated alkaline solution. The most conspicuous feature of the stained metaphases was the omission of silver staining in the secondary constrictions of chromosomes 1,9 and 16, and on the distal Yq. Our evidence indicates that the negative silver ...
Cowden R R - - 1976
A number of acidic dyes, including various fluorochromes, were substituted for biebrich scarlet in the modified Guard (1959) procedure, a regressive staining method which appears to demonstrate basic chromosomal proteins. These substitutions were made to test the possibility that dyes other than biebrich scarlet might provide advantages in sensitivity and/or ...
Pera F - - 1976
A method of labelling DNA in vivo with 5-bromodeoxyuridine (BrdU) is described. After 6 h permanent subcutaneous infusion of BrdU in rodents (adult Microtus agrestis, pregnant NMRI-mice), cell nuclei which have undergone DNA synthesis during the BrdU treatment can be differentiated from the nuclei of other cycle stages by means ...
Dancey J T - - 1976
Preparation of human marrow sections has been studied systematically in order to facilitate accurate identification of marrow cells. Both of the methods developed involve embedding marrow cores in methyl methacrylate. In one, acrolein fixation is followed by staining of deplasticized sections with eosine-y followed by azure II; in the other, ...
Tantravahi R - - 1976
Nucleolus organizer regions were detected by the Ag-AS silver method in fixed metaphase chromosomes from human and primates. In the human, silver was deposited in the secondary constriction of a maximum of five pairs of acrocentric chromosomes: 13, 14, 15, 21 and 22. The chimpanzee also had five pairs of ...
Tsenghi C - - 1976
Karyotypes were prepared from peripheral blood leukocytes in 77 couples in whom there was no apparent cause for recurrent spontaneous abortion. In addition to conventional staining, chromosomes were stained by the new technics for Q-, G-, or C-banding. Translocations were found in 5 of 154 persons (3.25% or 1:31 individuals). ...
Kanda N - - 1976
A successful modification of the BSG technique to reveal C and R bands simultaneously in human chromosomes is described. Conventional air dried preparations were treated first with 0.1 N HCl for 30 min at room temperature, then denatured in freshly prepared 3% aqueous solution of Ba(OH)2SH2O for 10 min at ...
Scheres J M - - 1976
A technique is described for staining centromeric areas and reverse, mainly telomeric bands in human chromosomes. With this "CT" technique karyotyping of C-banded metaphases is possible without previous or subsequent use of other banding methods. The method consists of an alkaline pretreatment at 60 degrees C with Ba(OH)2, followed by ...
Léonard A - - 1976
Densitometric analysis has been performed on mouse chromosomes from "in vivo" preparations stained with Giemsa after trypsinisation. The characteristic curves obtained by analysing the same chromosome after different durations of trypsinisation suggest that a trypsinisation step is not always necessary to get an analysable banding pattern. The best results are ...
Vagner-Capodano A M - - 1976
Human meiotic and mitotic chromosomes were studied with N-N' diethyl pseudoisocyanine stain. Following methylation and oxydation, the staining allowed microscopic observation of slides with both monochromatic light and fluorescence. In addition, stained preparations can be permanently conserved. Preceeded by diverse methods of chromosome denaturation or 5-BUDR incorporation, PIC lends itself ...
Distèche C - - 1976
When comparing the densitometric profiles of corresponding chromosomes registered from different metaphases or homologous pairs, one is always faced with the variability of their length and overall height. This makes difficult the quantitative comparison of a given chromosome treated by various staining procedures.--A simple and rapid method has been developed ...
Jurand A - - 1976
Methyl green-pyronin staining has been used for localization of RNA and DNA in chick and mouse embryonic tissues and in insect larval salivary glands. Glutaraldehyde or tricholoracetic acid-lanthanum acetate (TCA-LA) was used as fixative and paraffin wax or Araldite was used as embedding medium. For good results the following are ...
Lau Y F - - 1976
Application of sister chromatid differential (SCD) procedure on G1, S and G2 prematurely condensed chromosomes (PCC) of cells in the second and third cycle of DNA replication in medium containing BrdU reveals differential staining patterns characteristic of their respective stages in the cell cycle. These findings also suggest a structural ...
Mukherjee A B - - 1976
The fluorescent properties of drumsticks, drumstick-like appendages, and other nuclear bodies in the polymorphonuclear leukocytes from six human males and females were studied with the aid of the quinacrine-mustard staining technique. Both brightly and weakly fluorescent drumsticks (in females) and drumstick-like bodies (in males) were observed, and they were readily ...
Geraedts J P - - 1975
The positions of chromosomes 1 and Y inside human spermatozoa were determined by differential staining techniques. In 85/100 cells the two chromosomes were in close contact and in association with a vacuole. This observation is in contrast to previous findings for chromosome No. 9 and the Y-chromosome whose positions do ...
Crossen P E - - 1975
Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells ...
Niikawa N - - 1975
A standardized Q- and acridine orange (AO)-fluorescence dual marker technique was described. It involved preservation of unstained chromosome slides in a vacuum desiccator up to 18 months, Q-staining, destaining, and treatment in Hanks' solution, pH 5.1, at 85 degrees C for 13 min, and acridine orange staining. Q-markers were found ...
Hatfield J M - - 1975
Human chromosome spreads were stained with 3H-quinacrine and their fluorescence observed. The exact location of specific spreads on each slide was noted and photographs taken. Autoradiographs were then prepared so that the quinacrine fluorescence of any specific chromosome could be compared directly with the distribution of grains over the same ...
Angell R R - - 1975
Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromosome ...
Tsou K C - - 1975
6-Chloro-9-amino-2-hydroxyacridine was found to be a metabolite of both quinacrine and the antimalarial alkylating agent quinacrine mustard. Its structure was confirmed by a one-step reaction of quinacrine with 48 percent hydrobromic acid. The presence of this compound as a metabolite of quinacrine mustard suggests a possible in vivo activation mechanism ...
Cobb S R - - 1975
The mode of inheritance of the Unique Green phenomenon is investigated by a new technique. This involves a spectrometer which generates the stimulus in such a manner that the exact position that is neither blue-green nor yellow-green can be compared with neighbouring positions in the spectrum. This technique is used ...
Culbertson C G - - 1975
This report describes the use of the immunoenzymatic (peroxidase) method to identify the species and to stain distinctively the amebas in formalin-fixed paraffin-mounted sections. This permits the use of hematoxylin and eosin counterstaining. The method, now well developed by others for many purposes, is an alternative to immunofluorescence and seems ...
Holmquist G - - 1975
Metaphase chromosomes of D. melanogaster, D. virilis and D. eopydei were sequentilly stained with quinacrine, 33258 Hoechst and Giemsa and photographed after each step. Hoechst stained chromosomes fluoresced much brighter and with different banding patterns than quinacrine stained ones. In contrast to mammalian chromosomes, Drosophia's quinacrine and Hoechst bright bands ...
Dutt M K - - 1975
The paper embodies results of the use of 51 synthetic dyes, belonging to different chemical groups for staining of animal chromosomes following iodine-dye procedure. It has been found that some of these dyes can replace gentian violet, crystal violet and safranin when used after this procedure. It has further been ...
Zakharov A F - - 1975
Patterns of differential longitudinal spiralization of chromosomes induced by the addition of 5-bromodexyuridine (200 mug/ml) for 5-7 h before fixation of the cells were compared with the differential staining of normally spiralized chromosomes with giemsa stain in chromosomes of lymphocytes in human blood cultures. The patterns of differentiation of both ...
Diamond J R - - 1975
Two new types of staining regions exist aftet treatment with a modified version of the ammoniacal-silver technique (Howell et al., 1975), in addition to findings by many other researchers working on the chromosomal complement of Bos taurus. Densely stained regions appear to be associated with the centromeres of many of ...
Dallapiccola B - - 1975
The banding techniques currently employed in human cytogenetics for the identification of the individual chromosomes have been used to stain PHA lymphocytes and circulating leucocytes. The capacity of these techniques to localize singular chromosomes or chromosomal regions has been investigated. It has been observed that among the four major categories ...
Wulf H C - - 1975
A machine, performing hypotonic treatment and fixation of blood samples for chromosome analysis under standard conditions, is described. The machine has now handled specimens from 4,100 persons. 12 tubes are shaken at a rate of approximately 2,800/min, and simultaneously solutions for hypotonic treatment and fixation are added during 30-45 sec ...
Dallapiccola B - - 1975
The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly to that observed with quinacrine fluorescence. The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, ...
Uchida I A - - 1971
With the development of fluorescent staining techniques and their application to human chromosomes, identification of the entire human karyotype is now possible. Of considerable value are the elimination of the guesswork in the pairing of the C group and sex chromosomes and the recognition and characterization of the translocation chromosomes ...
Patil S R - - 1971
Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the pattern is quite similar to that obtained with quinacrine mustard ...
Singh R B RB Division of Plant Sciences, Institute of Advanced Studies, Meerut University, Meerut, - - 1971
Cytogenetic studies were made with particular emphasis on the sex-determining mechanism in Rumex acetosella (6 x = 42) and its hybrids (F 1, F 2, BC 1 and BC 2) with R. hastatulus (synthetic 4 x = 16 = 4 A +4 X = ♀ and 4 x = 18 ...
Bartkowiak E E Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, - - 1971
The results presented indicate that the sex determination mechanism in the Texas race ofR. hastatulus ♀ 2n = 10 (XX + 8A); ♂ 2n = 10 (XX + 8A)] is intermediate between theX/Y andX/A systems. In this race, sex is determined to some extent by theX/A balance, but theY chromosome ...
Singh M P - - 1970
Seeds of hulled barley (Hordeum vulgare) were germinated and then treated with LSD. Preparations of squashed root tips stained with Feulgen revealed extensive chromosomal aberrations, most of which were chromosome breaks. Nearly half of the breaks occurred in the region of the primary constriction.
MacInnes J W - - 1967
The degree of polarization of fluorescence from stretched Chironomus thummi polytene chromosomes, stained with low concentrations of acridine orange (AO), decreases with increasing temperature. The "half temperature" of this decrease (T((1/2)R)) is lower than the expected DNA thermal denaturation temperature (T(m)) by about 20 degrees C. T((1/2)R) is lowered as ...
Barnicot N A - - 1967
A method is described for bursting single, selected mitotic cells on a fluid surface. Cells from cultures of newt heart tissue were burst on dilute solutions containing potassium and sodium with and without added calcium and also on dilute calcium chloride solution. The material was negatively stained with uranyl acetate ...
Miller L D - - 1966
Typical elongate, beaded chromosomes have been observed in squash preparations of testicular tissue of the butterfly Speyeria aphrodite (Fabricius), the first demonstration of relatively uncondensed chromosomes in the Lepidoptera.
ROBBINS E - - 1961
Fixation of cell cultures with 1 per cent OsO(4) at constant pH and tonicity but variable cationic valence and dielectric constant causes profound changes in metaphase chromosomes. It is possible to make them disappear, flocculate, or show little change from the living cell in the phase contrast microscope. Conventional fixation ...
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