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Results 451 - 500 of 659
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Duan X S - - 1983
The technique of cell fusion, the method of sister chromatid differentiation (SCD), and the silver staining method modified by us that can show nucleolus organizing region (NOR) and sister chromatid differentiation at the same time have been used in the study of red muntjac chromosomes. Differential staining of sister chromatid ...
Burkholder G D - - 1983
To investigate a possible relationship between the core-like structures seen in silver-stained chromosomes (prepared by standard cytogenetic methods) and the scaffolds observed in histone-depleted chromosomes, the ability of the scaffold to stain with silver has been examined. Isolated chromosomes were histone-depleted by washing in ammonium acetate or by spreading the ...
Bainbridge D R - - 1983
Counterstaining with the chromosomal dye Hoechst 33258 is a simple procedure that provides an excellent general purpose nuclear counterstain for immunofluorescent work. Benefits from its use include the ready identification and orientation of structures, differentiation of lymphoid and non-lymphoid cells and easy assessment of frequency in specifically labelled cellular subpopulations. ...
Handsfield H H - - 1983
Treponema pallidum was demonstrated in a cutaneous, tertiary syphilitic lesion by indirect immunofluorescence microscopy, but not by darkfield microscopy illumination or silver stain. The numerous organisms observed by this method may help explain the histologically vigorous tissue reaction in tertiary syphilis, despite the scarcity of organisms demonstrable by other methods.
Fantes J A - - 1983
A method of producing an enriched sample of human Y chromosomes from peripheral blood lymphocytes is described. Metaphase chromosomes were prepared from peripheral blood lymphocytes donated by 17 normal male individuals. A suspension of chromosomes in a polyamine buffer was produced from each sample, stained with the fluorescent dye Hoechst ...
Southern J A - - 1983
A method is described for the preparation of foetal calf serum from blood drawn by heart puncture from calf foetuses obtained from a local municipal abattoir. This method differs from that previously described in that the Seitz Supra EK filter pads used in preliminary purification steps have been abandoned. These ...
Lichtenberger M J - - 1983
Staining G-bands of chromosomes can be carried out in 2 1/2 minutes using a mixture of Giemsa solution with a special diluting preparation. The staining is completely reversible without any damage to the chromosomes. Moreover, an improved method for staining Q-bands with atebrin and mounting the preparation in sucrose is ...
McEwen B F - - 1983
Zinc-induced tubulin sheets without microtubule-associated proteins (MAPs) were assembled from tubulin purified by phosphocellulose chromatography. Large, open sheets were obtained in five-minute incubations at pH 5.7. Electron micrographs of negatively stained sheets showed a protofilament arrangement similar to that observed for zinc-induced sheets with MAPs but with altered lattice parameters. ...
Morin-Faure J - - 1983
The reaction of cis-dichlorodiamino platinum (cis-Pt) with the human metaphase chromosome was studied using both Giemsa staining and the action of anti-cis-Pt antibody. Two kinds of experiment were performed: (1) cis-Pt was placed in contact with cells in culture (in vivo tests), (2) cis-Pt was placed in contact with chromosomes ...
Morton C C - - 1983
The stain intensity of the nucleolus organizer regions (NORs) of acrocentric chromosomes was correlated positively with incorporation of [3H]uridine into 18S rRNA and 28S rRNA from cultured diploid human skin fibroblasts. An analysis of these data from twins by a path model indicated that no other common genetic or environmental ...
Mezzanotte R - - 1983
Human cytological preparations have been digested with Alu I restriction endonuclease (Endo R Alu I) and subsequently stained with acridine orange. Metaphase chromosomes treated in this way revealed a clear-cut bright/ dull longitudinal differentiation. It has been postulated that these cytological findings could be related to the selective digestion, by ...
Bijman J T - - 1983
Metaphase chromosomes from Chinese hamster V-79 cells have been stained with propidium iodide and their DNA content was measured by flow cytometry. The chromosomes in suspension must be stabilized during the preparation to preserve their morphological integrity. In order to obtain high resolution in the flow karyotype, several modifications were ...
Murata M - - 1983
The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poor stained by common stains, and difficult to ...
Madura J A - - 1983
A new technique has been described that permits easy location of the rectal stump at the time of colostomy closure for the Hartmann procedure. The routine use of a polypropylene (Prolene) suture to define the location of the rectal stump eliminates tedious pelvis dissection, which could injure the ureter or ...
Gold J R - - 1983
Refinements to a simple, one-step silver staining technique for nucleolar organizing regions are described. These include fixation of silver stained material with sodium thiosulfate and standardization of silver development conditions for different groups of vertebrates. The central advantages to the method are that it is rapid, reliable, simple, and inexpensive. ...
Mezzanotte R - - 1983
Human metaphase chromosomes were treated with HaeIII, HindIII, EcoRI, and AluI restriction endonucleases and subsequently stained with either Giemsa or ethidium bromide. The results obtained seemed to suggest that the structural organization of specific chromosome regions can play a primary role in determining the cytological effect after digestion with specific ...
Howard-Peebles P N - - 1983
Silver-stained preparations of cultured lymphocytes obtained from 12 pure-bred dogs revealed the presence of nucleolus organizer regions (NORs) on four to seven chromosomes in females and five to eight chromosomes in males. All seven males had a NOR on the Y chromosome. The telomeric location of the NORs on the ...
Cornforth M N - - 1983
A relatively simple method has been developed to improve the resolution for measuring breaks produced in interphase chromosomes by X rays or other agents following the induction of premature chromosome condensation (PCC). Mitotic HeLa cells, which induce PCC when fused with interphase cells, were obtained from cultures grown for several ...
Buhariwalla F - - 1983
In a study requiring rapid diagnosis of the chromosomal sex of mice, standard staining methods for mouse chromosomes were evaluated for speed, simplicity, and the quality of staining. None of the techniques previously used for mouse chromosomes were adequate. However, a DAPI-distamycin A method for human karyotyping was successfully adapted ...
Li Y S - - 1983
A case of erythroleukaemia with homogeneously staining regions on chromosomes is described. The number of chromosomes in bone marrow cells varied from 41 to 46 with a mode of 43. A variety of structural and numerical chromosomal abnormalities was observed. Two marker chromosomes with homogeneously staining regions were found in ...
Verma R S - - 1983
The morphology of nucleolus organizer regions (NORs) of human acrocentric chromosomes has been studied in a family by QFQ, RFA, and NSG banding techniques. The pale green colour seen by RFA was found darkly stained by NSG. However, in some instances, NOR regions were expressed by NSG technique but the ...
Donlon T A - - 1983
The DA/DAPI technique has been found to be useful in the identification of specific chromosomal regions on human chromosomes. The realization that distamycin A (DA) is no longer commercially available has necessitated the development of an alternative technique. We describe a technique, MG/DAPI, which substitutes the AT-specific dye methyl green ...
Goyanes V J - - 1983
BrdU-substituted chromosomes of Don cells were differentially stained with ammoniacal silver carbonate and analyzed by electron microscopy. The results obtained led us to the following conclusions: (1) the differential impregnation of BrdU-substituted chromatids with silver is mainly due to a preferential extraction of non-histone proteins caused by UV-irradiation and incubation ...
Langlois R G - - 1982
Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in ...
Lebo R V - - 1982
We describe the design and operation of a dual laser fluorescence activated chromosome sorter that can analyze and sort chromosome suspensions stained with two complementary DNA stains. We used two 18-watt argon-ion lasers to provide maximal excitation of the DNA-specific stains Hoechst 33258 and chromomycin A3. The lasers are focused ...
McClure J - - 1982
In patients with chronic renal failure estimations of the extent of calcification fronts delineated by in vivo tetracycline labelling correlated well with estimates obtained after staining the fronts with haematoxylin dye (Raina method). Similarly there was a good correlation between the Raina method and the demonstration of calcification fronts with ...
Curtis D - - 1982
Metaphase chromosomes were stained in a routine G-banding procedure with 39 basic dyes of varied structures substituted for the Giemsa stain. Staining outcomes were categorized as: overstained, differentially stained, trivially or unstained. Certain structural features of the dyes were described numerically, namely, largest conjugated fragment (LCF), conjugated bond number (CBN) ...
Zankl H - - 1982
The association pattern and NOR silver staining of the acrocentric chromosomes was studied in normal and hypodiploid cell lines of four meningioma patients. In three cases the hypodiploid cells showed a more increased association frequency or NOR silver staining or both than the normal ones, indicating a compensatory mechanism between ...
De Backer W - - 1982
A 32-year-old woman who ingested thallium sulfate was treated with combined hemoperfusion-hemodialysis. Classical therapy, including Prussian Blue, was also administered. The clearance rate of the hemoperfusion and hemodialysis were 139 and 47 mL/min, respectively. The efficiencies of these different methods were compared with each other and with the classical methods. ...
Tucci S M - - 1982
In previous immunohistochemistry studies, cyclic guanosine monophosphate (cGMP) has been found in polytene chromosomes of D. melanogaster, cGMP has not been found in mammalian metaphase chromosomes, but this could be due to loss of cGMP during staining. Thus the effect of different fixation techniques on the immunohistochemically detectable cGMP associated ...
van Prooijen-Knegt A C - - 1982
The properties of aminoalkylsilane-treated glass slides for the preparation of metaphase spreads and their staining quality have been studied and compared with those of slides which had only been cleaned in ethanol/ether. The parameters investigated were: (1) the average area of metaphases from cultures of blood from both healthy donors ...
Bosman F T - - 1982
A method for the preparation of ultrathin sections of metaphase chromosomes is described. This method was applied to human metaphase chromosomes, which were immunocytochemically stained with anti-DNA and anti-ribonucleoprotein antibodies, derived from patients with auto-immune disease. Conventionally prepared metaphase spreads as well as cytocentrifuge preparations of chromosome suspensions were studied. ...
Stoehr M - - 1982
A reproducible, simple and rapid protocol was developed to prepare mono-dispersed chromosome suspensions for flow cytometric analysis. The procedure basically employes: 1. twice rinsing of monolayer cultures to eliminate dead cells and cellular debris, 2. mild fixation of mitotic cells with 1% acetic acid, and 3. ultra sonic treatment to ...
Singh B K - - 1982
Heterochromatin distribution in metaphase chromosomes of three morphologically and karyologically similar members of Drosophila, D. s.sulfurigaster, D. s.bilimbata and D. pallidifrons was studied by the Hoechst 33258 staining method. Comparison showed remarkable uniformity of H-heterochromatin among the three members except for in their Y-chromosomes. On the basis of these observations ...
González-Gil G - - 1982
This paper analyses the effect of acid hydrolysis on the differential Giemsa staining of 5-bromo-2'deoxyuridine (BrdU) substituted chromatids in human and plant chromosomes, after treatment with a fluorochrome and light. Human lymphocytes and Allium cepa L. root tips were grown in BrdU for two or three cell cycles. Lymphocytes spreadings ...
Johnson P W - - 1982
Electron micrographs of acetic acid-alcohol fixed, unstained human metaphase chromosomes have been analyzed with an image system. Electron micrographs were first digitized from over a million picture elements into a range of 64 gray levels which correspond to mass distribution. The obtained numerical data were then processed by programs which ...
Burkholder G D - - 1982
Chinese hamster metaphase chromosomes, stained with ammoniacal silver and examined by electron microscopy, were covered with fine silver grains of variable size. In addition, many chromosomes contained linear aggregates of silver grains running continuously from one end of each chromatid to the other, forming a core-like structure. Extensive deposits of ...
Utsumi K R - - 1982
Giemsa-stained chromosomes as prepared for light microscopy, and including G-banded, C-banded, and FPG-stained chromosomes, were examined by scanning electron microscopy. Although suitable for light microscopy, these chromosomes were too flat for a close examination of their fine structure by scanning electron microscopy. The surface of Giemsa-positive regions was rough and ...
Fernandez-Piqueras J - - 1982
Differential staining of the X chromosome of Orthoptera, which normally cannot be distinguished from the autosomes during the more condensed stages of meiosis, has been achieved by a silver impregnation procedure involving a pretreatment with 2 x SSC at 60 degrees C. The results suggest that the saline citrate solution ...
Zakharov A F - - 1982
Activity of nucleolar organizer regions (NORs) was studied in cultured blood lymphocytes from 20 monozygotic (MZ) and 20 dizygotic (DZ) twin pairs. The number of Ag-stained NORs, the degree of staining, and the frequency of acrocentric associations were used as criteria of the NOR activity, the acrocentric chromosomes being identified ...
Tymowska J - - 1982
A cytological analsis of the recently discovered tetraploid species Xenopus epitropicalis was carried out, using, in addition to the classical orcein method, silver staining and alkaline Giemsa banding techniques. The chromosome number of X. epitropicalis was found to be 40. The chromosomes can be grouped in to sets of four ...
Turner B M - - 1982
A mouse monoclonal IgM antibody against the core histone H2B has been shown, by indirect immunofluorescence, to stain metaphase chromosomes from a variety of cultured cell types. Experiments carried out with human HeLa cells showed that the intensity of staining varied along the length of chromosome arms giving in some ...
Zakharov A F - - 1982
Polymorphisms of the NORs as tested by Ag-staining of metaphase G-banded chromosomes were investigated in cultured blood lymphocytes of karyotypically normal individuals from the Moscow population. The study of cell-to-cell variability in the number of Ag-stained NORs carried out on 14 monozygotic twin pairs showed the phenomenon to have some ...
Mayr B - - 1981
Nucleolus organizer regions (NORs) of the peripheral blood lymphocytes (PBL) of 9 mammalian species were analyzed by means of a silver-staining procedure. Species-specific NOR patterns were demonstrated. The number of NOR chromosomes was positively correlated with the nucleolar coefficient, and negatively correlated with the relative frequencies of uninucleolar cells in ...
Young B D - - 1981
A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of ...
Dutt M K - - 1981
This paper presents a very simple and reliable procedure for the staining of animal chromosomes employing dyes, such as pyronin G, acridine red, rhodamine B, rhodamine 3GO, belonging to aminoxanthene group and brilliant cresyl blue and methylene violet 3RD, belonging to quinone-imine group. The procedure has been tried on sections ...
Arrau J - - 1981
The onset and evolution of the meiotic prophase were assessed by histological and cytological techniques in fetal and neonatal ovaries obtained from day 13 postcoitum to day 11 postpartum in the golden hamster (Mesocricetus auratus). The histological technique based on the Feulgen stain and the cytological technique based on the ...
Denton T E - - 1981
Silver selectively stains nucleolar organizer regions (NORs) distributed on the D and G chromosomes of man. The number of NORs is fairly constant for a given individual but is highly variable within populations. When 2540 metaphases from lymphocytes were examined from 127 normal subjects, a mean NOR number of 7.3 ...
Schnedl W - - 1981
The utility of a newly synthesized chemical variation of DAPI (4'-6-diamidino-2-phenyl-indole), D 287/170, for differential staining of constitutive heterochromatin in man is demonstrated. Direct staining of human chromosomes with D 287/170 results in brilliant fluorescence of the paracentromeric C-band of chromosome 9, of a proximal short-arm segment of chromosome 15, ...
Klasen M - - 1981
The fluorescence of Y-bodies and brightly fluorescing autosomal heterochromatin in quinacrine mustard stained interphase nuclei treated with distamycin A is more brilliant than in nuclei stained only with quinacrine and mustard. In the present study examinations with the aid of this method in several probands and different tissues are described. ...
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