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Results 451 - 500 of 613
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Arrau J - - 1981
The onset and evolution of the meiotic prophase were assessed by histological and cytological techniques in fetal and neonatal ovaries obtained from day 13 postcoitum to day 11 postpartum in the golden hamster (Mesocricetus auratus). The histological technique based on the Feulgen stain and the cytological technique based on the ...
Denton T E - - 1981
Silver selectively stains nucleolar organizer regions (NORs) distributed on the D and G chromosomes of man. The number of NORs is fairly constant for a given individual but is highly variable within populations. When 2540 metaphases from lymphocytes were examined from 127 normal subjects, a mean NOR number of 7.3 ...
Schnedl W - - 1981
The utility of a newly synthesized chemical variation of DAPI (4'-6-diamidino-2-phenyl-indole), D 287/170, for differential staining of constitutive heterochromatin in man is demonstrated. Direct staining of human chromosomes with D 287/170 results in brilliant fluorescence of the paracentromeric C-band of chromosome 9, of a proximal short-arm segment of chromosome 15, ...
Klasen M - - 1981
The fluorescence of Y-bodies and brightly fluorescing autosomal heterochromatin in quinacrine mustard stained interphase nuclei treated with distamycin A is more brilliant than in nuclei stained only with quinacrine and mustard. In the present study examinations with the aid of this method in several probands and different tissues are described. ...
Jørgensen A L - - 1981
The length of 44 silver-stained human autosomal pachytene complements was shown to vary from about 300 micrometer to at least 535 micrometer. The lengths of the individual 22 autosomal chromosomes of eight complements representing this interval was measured and the relative lengths calculated. For most of the chromosomes a 1:1 ...
Schnedl W - - 1981
R banding of porcine chromosomes by chromomycin A3 plus distamycin A and DAPI (DA-DAPI) revealed two distinct types of heterochromatin: The GC-rich centromeric heterochromatin of the biarmed autosomes (Nos. 1-12) and of the X chromosome exhibited bright chromomycin A3 fluorescence, while the heterochromatin of the acrocentric chromosomes (Nos. 13-18) stained ...
Yamasaki N - - 1981
The lampbrush loops of the Y chromosome in primary spermatocytes of Drosophila hydei can be stained in a site specific manner employing a modified Giemsa technique. In this communication it is shown that pronase pretreatment changes the staining properties of the various Y loops as well as of the X ...
Taylor E F - - 1981
The silver staining patterns of the nucleolus organizer regions (NORs), an indication of rDNA transcriptional activity, were studied in metaphases from lymphocyte cultures of 20 karyotypically normal members of three families selected for a large sibling number or a monozygotic twin pair. Quinacrine polymorphic markers and bands were used to ...
Buys C H - - 1981
Distamycin A/DAPI staining and sequential C-banding of human lymphocyte chromosomes reveals the regular occurrence of differentially staining subfractions of chromosome 9 constitutive heterochromatin. These subfractions are regionally organized as two subsegments: a distal one, which fluoresces brightly with DAPI after preincubation with distamycin A and a proximal one, which stains ...
Bonaccorsi S - - 1981
Prophase chromosomes of Drosophila hydei were stained with 0.5 microgram/ml Hoechst 33258 and examined under a fluorescence microscope. While autosomal and X chromosome heterochromatin are homogeneously fluorescent, the entirely heterochromatic Y chromosome exhibits an extremely fine longitudinal differentiation, being subdivided into 18 different regions defined by the degree of fluorescence ...
Newhouse J H - - 1981
Percutaneous nephrostomy can be accomplished by using a trocar-cannula assembly without resorting to serial dilations of the tube tract or utilizing stiff catheters. After puncture the trocar is removed and a catheter inserted through the cannula; then the cannula is removed. A large number of these procedures have been performed ...
Morton C C - - 1981
Patterns of NOR activity in 640 metaphase spreads from twelve monozygotic (MZ) and eight dizygotic (DZ) twin pairs were studied to evaluate the heritability of this chromosomal heteromorphism. NORs were stained by a modification of the Ag-AS technique and counterstained with quinacrine mustard dihydrochloride to facilitate chromosome identification and assess ...
Stoll M - - 1981
The method described here has the advantage of presenting a clear image of both chromosomes and silver grains. Chromosomes are stained through the emulsion with Hoechst 33258. As two different sources of light are employed-epi-ultraviolet illumination and transmitted visible light-a separate photographic record of each optical plane can be obtained ...
Satya-Prakash K L - - 1981
A silver-staining technique revealed the core morphology of metaphase chromosomes of irradiated CHO cells with chromatid lesions (breaks, gaps). These cells were photographed before and after silver staining. As a rule, the core was not continuous in chromatid gaps, suggesting that the chromatid is broken in many so-called gaps. Ten ...
Sumner A T - - 1981
The distribution of quinacrine in relation to Q-banding on CHO chromosomes has been investigated using X-ray microanalysis. Technical problems involved in this type of experiment were studied in detail. It was necessary to use a solution of quinacrine acetate in acetic acid to ensure that the only chlorine detectable in ...
Foresti F - - 1981
An analysis of the nucleolar organizer region(NOR) by silver staining of five species of the order Gymnotiformes, Gymnotus carapo, Apteronotus albifrons, Sternopygus macrurus, Eigenmannia virescens, and Eigenmannia sp., is reported. The five species presented only one pair of homologs bearing NORs. In G. carapo, A. albifrons, and S. macrurus the ...
Wang H C - - 1981
The axial element of sex chromosomes in the sex vesicle of rat and mouse spermatocytes has been visualized under the light microscope by the dye Coomassie brilliant blue (CBB). After staining in the CBB solution for 3-10 minutes, the axial elements appeared as darkly stained threads in the sex vesicles, ...
Schmid M - - 1981
Lymphocyte cultures from the gorilla, chimpanzee, and orangutan were treated with the oligopeptide antibiotic distamycin A. This AT-specific DNA-ligand induces a distinct undercondensation in the quinacrine-bright heterochromatin of the gorilla and chimpanzee. This is also the case in human lymphocyte cultures. Distamycin A further causes an undercondensation in the nonheterochromatic ...
Sillar R - - 1981
A new method for the preparation of metaphase chromosomes for flow analysis has been evaluated. It has been shown that this method, which involves detergent lysis of metaphase cells and polyamines to stabilize the DNA, yields lower coefficients of variation and background levels in the DNA histograms than is currently ...
Okada T A - - 1980
A protein chromosome scaffold structure has been proposed that acts as a structural framework for attachment of chromosomal DNA. There are several troubling aspects of this concept: (1) such structures have not been seen in many previous thin-section and whole-mount electron microscopy studies of metaphase chromosomes, while they are readily ...
Thomsen J L - - 1980
The possible significance of some factors with regard to their effect on Y-chromosome detection in blood stains was investigated. (1) It was found that when using centrifugal force instead of a pipette for the extraction of stains on cotton cloth a much higher number of leukocytes was achieved during the ...
Thomsen J L - - 1980
The present investigation is an extension of an earlier work on the effect of various temperatures on Y-chromosome detection. The deteriorating effect of storage at 53 degrees C was again demonstrated. Liquid blood samples stored at 5 degrees C were better preserved with regard to Y-chromosomes than those stored at ...
Latt S A - - 1980
A number of DNA-binding dyes, with spectral properties making them suitable as components of energy donor-acceptor pairs, are described. If such pairs are used to stain metaphase chromosomes, and if the energy acceptor (e.g., actinomycin D or methyl green) has a binding specificity opposite to the binding or fluorescence specificity ...
Chadov B F - - 1980
Herein is described an attempts to establish chromosome pairing-interchange relationships in Drosophila melanogaster female. For this purpose, the formation of half-translocations was studied in XXY and XX females bearing compounds of the second pair of autosomes. With respect to XXY females, it was expected that the free Y chromosome would ...
Hizume M - - 1980
A method for the specific detection of the nucleolus organizing regions (NORs) of plant chromosomes has been developed employing enzymatic maceration and successive flame-drying for chromosome spreading and incubation with aqueous 50% AgNO3 at 55-60 C. When this method was applied to metaphase chromosomes the NORs were specifically discriminated as ...
Stöhr M - - 1980
Chromosomes from rat kangaroo (PTK) and chinese hamster (CHV 79) cells have been prepared for quantitative flow-cytometric analysis. The preparation time was otimized down to 30 (PTK) and 40 min (CHV 79). DAPI was used as a AT-sensitive fluorescent dye to stain for monoparameter DNA measurements. Simultaneous two-parameter DNA-protein analysis ...
Winking H - - 1980
Silver-NOR staining has been applied to mouse (Mus musculus) chromosomes of different feral populations from Italy, Yougoslavia, and Germany and to chromosomes of M. spretus, a closely related species, from Portugal. In addition to the known pattern of proximal sites on smaller autosomes, terminal sites with silver-NORs on chromosomes 4 ...
Schmid M - - 1980
The chromosomes of 26 species of Anura from variously highly envolved groups were analysed with the fluorescent GC-specific antibiotics mithramycin and chromomycin A3 as well as with the AT-specific quinacrine. The mithramycin- and chromomycin A3-stainings generally resulted in a pattern of the constitutive heterochromatin opposite to the one obtained with ...
Zankl H - - 1980
The pattern of acrocentric chromosomes was studied in 190 normal and 190 trisomic cells from a patient with trisomy 21 mosaicism. No significant differences were observed in the total numbers of associations, the numbers of mitoses with one, two, or three associations, or the numbers of associations in which more ...
Wiese D A - - 1980
Root tips were fixed in ethanol-acetic acid and in neutral formalin containing a mercaptide-forming agent to protect thiols from autoxidation. Serial paraffin sections 2 micrometers thick were labelled for thiols, disulfides and both together with the azogenic maleimide 'APM'. Nuclei were stained somewhat lighter for thiols, and nucleoli much darker, ...
Lau Y F - - 1980
The patterns of differential staining based on the effects of BrdU-substitution in chromosomal DNA have been examined in both metaphase chromosomes and prematurely condensed chromosomes (PCC) of interphase Chinese hamster cells. Results indicate that differential staining may be obtained in chromosomes from all stages of the cell cycle and correspond ...
Zankl H - - 1980
The intensity of the silver staining of nucleolus organizing regions (NORs) and the frequency of chromosomal associations were studied before and after antithyroid treatment in seven and eight hyperthyroid patients, respectively. For one patient, the NOR staining was significantly more intense after treatment, while for another the opposite result was ...
Satya-Prakash K L - - 1980
A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mitotic chromosomes in ...
Lawrie S S - - 1980
A method is described for producing fluorescent bands on human chromosomes by staining with quinacrine after hybridisation in situ. The advantages of the method include the eslimination of artefacts arising from staining before hybridisation, the fact that there is no reduction in sample number between staining and autoradiography, the ease ...
Curtis S K - - 1980
A series of experiments was undertaken in which cells dissociated from the abdominal lymph nodes of mice were lightly centrifuged into slides and fixed either wet or after drying in 70% ethanol, 1% glutaraldehyde, 1% formaldehyde, or neutral formalin. Three fluorescent cytochemical methods were evaluated: staining of DNA with mithramycin; ...
Sahar E - - 1980
The ability of electronic energy transfer and direct binding competition between pairs of dyes to enhance contrast in human or bovine metaphase chromosome staining patterns is illustrated, and the relative effectiveness of these two mechanism compared. The existence of energy transfer between quinacrine or 33258 Hoechst and 7-amino-actinomycin D in ...
Lin C C - - 1980
Metaphase chromosomes stained with acridine orange exhibit uniform yellow-green fluorescence. Chromosome preparations treated with the non-fluorescent A-T specific antibiotic distamycinA prior to acridine orange staining exhibit longitudinal fluorescent banding patterns similar to those produced by a number of fluorescent R-band techniques. Similarly, chromosome preparations treated with the non-fluorescent G-C specific ...
Kaiserman M Z - - 1980
Chinese hamster metaphase chromosomes, subjected to prolonged hypotonic pretreatment and subsequently stained with ammoniacal silver, contained a darkly-stained core-like structure in each chromatid, surrounded by a halo of dispersed chromatin which was pale yellow to brown in color. The core was variable in its appearance, ranging from a continuous linear ...
Langlois R G - - 1980
The cytochemical properties of metaphase chromosomes from Chinese hamster and human cells were studied by flow cytometry. This technique allows precise quantitation of the fluorescence properties of individual stained chromosome types. Chromosomes were stained with the following fluorescent DNA stains: Hoechst 33258, DAPI, chromomycin A3, ethidium bromide, and propidium iodide. ...
Wachtler F - - 1980
Chromosome preparations from 67 randomly selected healthy individuals were consecutively stained with silver, mithramycin, pentamidine-DAPI, and quinacrine mustard. In the short arm of the chromosome 15 up to six regions, which were highly polymorphic, apparently independently of each other, could be detected by the given methods. The frequency of occurrence ...
Ghosh P K - - 1979
Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing them to UV light, treating them with ...
Buys C H - - 1979
Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human chromosome 16 was indicated. ...
Dev V G - - 1979
Cytogenetic analysis of a balanced reciprocal translocation t(5;13) carrier and her unbalanced 5p- conceptus was carried out. This carrier mother had previously given birth to a child with cri-du-chat syndrome. Silver staining demonstrated the breakpoint on 13p within the nucleolus organizer region (NOR). In the carrier, the NORs both at ...
Takayama S - - 1979
The direct staining of BrdU-substituted Chinese hamster chromosomes in a Na2HPO4-Giemsa solution without any pretreatments resulted in a B-dark type SCD istituted (TB) chromatids stained light. Detailed examinations of the staining process suggested that the Na2HPO4 solution acts to collapse chromosomes whereas the Giemsa dye works to reconstruct the collapsed ...
Limon J - - 1979
Two differently stained regions of lateral asymmetry were observed in the long arm of the human Y chromosome, following FPG staining. The first asymmetry was confined to band q12 of the long arm. The second asymmetrically stained region was located at the junction between bands q11 and q12. In the ...
Hayashi K - - 1979
A rapid and simple disc electrophoretic technique for the determination of saliva esterase (Set) types is described. The frequency of Set types was F 16.4%, FS 49.8% and S 33.8%. The estimated gene frequency of Set-1F was 0.413 and of Set-1S was 0.587. An accurate determination of Set types was ...
Howell W M - - 1979
Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selectively reduced the silver and stained black while the surrounding ...
Tabuchi K - - 1979
A method is described for producing banding patterns with methyl green-pyronin (MGP) stain in chromosomes of fibrosarcoma cells. 1) The stain was made by mixing equal volumes of 2% aqueous pyronin G, 2% aqueous methyl green, distilled water, and 0.1 M acetate buffer (pH 5.7). 2) Treatment with colcemide and ...
Hammerman I S - - 1979
The simplest possible model of the sex determination process adding autosomal influence to a minimal number of sex chromosomes was developed to explain matings of Tilapia (Sarotherodon) species. Eighteen different genotypes, each having two autosomes (AA, Aa, or aa) and two sex chromosomes (WX, WY, WW, XY, XX or YY) ...
Lakhotia S C - - 1979
Larval brain ganglia of Drosophila nasuta were cultured in vitro in the presence of 5-bromodeoxyuridine for 1 or 5 h at 24 degrees C and the air-dried chromosome preparations stained by the Hoechst 33258-Giemsa technique to reveal bromodeoxyuridine induced sister chromatid differentiation. In 1 h as well as 5 h ...
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