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Spits C C Research Group Reproduction and Genetics, Vrije Universiteit Brussel, 1090 Brussels, Belgium Centre for Reproductive Medicine, UZ Brussel, 1090 Brussels, - - 2014
Do cleavage-stage embryos obtained from oocytes matured in vitro after pre-incubation with a phosphodiesterase inhibitor (IBMX) carry more chromosomal abnormalities than those generated from oocytes matured in vivo? The rate and type of chromosomal abnormalities in normally developing cleavage-stage embryos generated with an in vitro maturation (IVM) system including pre-incubation ...
Fujii T T Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka, Japan; Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, - - 2014
The giant egg (Ge) locus is a Z-linked mutation that leads to the production of large eggs. Cytological observations suggest that an unusual translocation of a large fragment of the W chromosome bearing a putative egg size-determining gene, Esd, gave rise to giant egg mutants. However, there is currently no ...
Arcarons N N Universitat Autònoma de Barcelona, Bellaterra, - - 2014
It has been previously described that a simple treatment with medium containing elevated NaCl or sucrose concentrations increases the cryotolerance and developmental competence of in vitro-matured porcine oocytes after vitrification and parthenogenetic activation (Lin et al. 2009 Reprod. Fertil. Dev. 21, 338-344). This work was designed to study whether the ...
Peyrás S D SD Department of Genetics, University of Córdoba, Cordoba, - - 2014
Chromosomal abnormalities were related to embryo developmental failures in cattle, being highly increased in IVF-produced compared with multiple-ovulation embryo transfer-produced embryos. The origin of this difference remains unclear, but they were related to the suboptimal environmental culture laboratory conditions. We studied the influence of 3 different maturation media in the ...
Pruvost Nicolas B M NB Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, - - 2014
The scarcity of parthenogenetic vertebrates is often attributed to their 'inferior' mode of clonal reproduction, which restricts them to self-reproduce their own genotype lineage and leaves little evolutionary potential with regard to speciation and evolution of sexual reproduction. Here, we show that for some taxa such uniformity does not hold. ...
Jo Jun Woo JW Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, - - 2014
Necrostatin 1 (Nec1) is widely used in disease models to examine the contribution of receptor-interacting protein kinase 1 in cell death. The biological actions of Nec1 are blocking necrotic cell death. The purpose of this study was to investigate whether adding Nec1 into in vitro maturation (IVM) media, followed by ...
Karamysheva Tatyana T Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics , Jena , Germany - - 2014
Abstract Small supernumerary marker chromosomes (sSMC) are found about four times more frequently in subfertile compared to the general population. The reason for this finding is still unclear. However, a connection of interphase architecture and genome function is suggested. And as we found in a previous study the presence of ...
Garmendia Alfonso A Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Valencia, - - 2014
A new contact zone between Centaurea aspera and Centaurea seridis was found in Morocco. Chromosome counts and flow cytometry showed that both taxa were tetraploid (4x = 44). A literature review and morphometric analysis established that C. aspera corresponds to the autopolyploid C. aspera subsp. gentilii and C. seridis corresponds ...
Hering D M DM University of Warmia and Mazury in Olsztyn, Department of Animal Genetics, 10-719 Olsztyn, - - 2014
In artificial insemination industry bulls producing high volume of semen with relatively high concentration of sperm are very desirable since they ensure stable production of commercial straws especially in case of top bulls. The aim of the study was to screen the entire bull genome to identify markers and candidate ...
Chatziparasidou A A Embryolab SA, IVF Unit, Kalamaria, Thessaloniki, Greece; Embryolab Academy, Kalamaria, Thessaloniki, - - 2014
Males with abnormal karyotypes and subgroups of fertile and infertile males with normal karyotypes may be at risk of producing unbalanced or aneuploid spermatozoa. Biological, clinical, environmental and other factors may also cause additional sperm aneuploidy. However, increased risk of sperm aneuploidy is directly related to chromosomally abnormal embryo production ...
Graham Erin R - - 2014
It was recently discovered that symbiotic algae in the eggs of the salamander Ambystoma maculatum translocate fixed carbon from photosynthesis to developing embryos. Fixed carbon translocation was shown in embryos at one time point during development however, it was unknown if fixed carbon translocation occurs throughout all developmental stages. In ...
Hosseini S M SM Department of Reproduction and Development, Reproductive Biomedicine Centre, Royan Institute for Biotechnology, ACECR, Isfahan, - - 2014
Despite many advances in the field of oocyte cryopreservation, the adverse effects of cryopreservation on oocyte competence are still an open challenge in most mammalian species. Using ovine in vitro-matured oocytes, the differential effects of vitrification steps, embryo production methods, and parental origin of pronuclei were systemically investigated to unravel (1) ...
Ramasamy Ranjith R Scott Department of Urology, Baylor College of Medicine, Houston, Texas; Center for Reproductive Medicine, Baylor College of Medicine, Houston, - - 2014
Male factor infertility is a relatively common condition, affecting at least 6% of men of reproductive age. Typically, men with unknown genetic abnormalities resort to using assisted reproductive techniques (ART) to achieve their reproductive goals. Infertile men who father biological children using ART could have a higher incidence of aneuploidy, ...
Mason Annaliese S AS School of Agriculture and Food Sciences, The University of Queensland, Brisbane 4072, Australia; Centre for Integrative Legume Research, The University of Queensland, Brisbane 4072, Australia. Electronic address: - - 2014
Unreduced gametes (gametes with the somatic chromosome number) are known to facilitate polyploid formation. Unreduced gametes result from a plethora of different mechanisms across different taxa, suggesting that the ability to produce unreduced gametes has evolutionary utility. Heritable genetic variation for unreduced gamete production has been observed, thereby providing an ...
Wang Yanyun Y Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, - - 2014
Males absent on the first (MOF) was originally identified as an essential component of the X chromosome dosage compensation system in Drosophila melanogaster, and is also a member of the MYST family of histone acetyltransferases. MOF has been extensively studied in D. melanogaster and mammals. However, whether MOF is involved ...
Castillo Almudena A Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, Córdoba E-14080, - - 2014
Cytoplasmic male sterility (CMS) results from incompatibility between nuclear and cytoplasmic genomes, and is characterized by the inability to produce viable pollen. The restoration of male fertility generally involves the introgression of nuclear genes, termed restorers of fertility (Rf). CMS has been widely used for hybrid seed production in many ...
Xiao Jun J Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 36 Lu Shan Road, Yue Lu District, Changsha 410081, Hunan, People's Republic of - - 2014
Distant hybridization can combine together the genomes of different species, which leads to changes of the offspring in phenotypes and genotypes. In this study, we successfully establish a fertile hybrid lineage by intergeneric hybridization of female blunt snout bream (BSB, Megalobrama amblycephala)×male topmouth culter (TC, Culter alburnus) and investigate some ...
Satou Yutaka Y Department of Zoology, Graduate School of Science, Kyoto University, Sakyo, Kyoto, 606-8502, Japan. - - 2014
Because self-incompatibility loci are maintained heterozygous and recombination within self-incompatibility loci would be disadvantageous, self-incompatibility loci are thought to contribute to structural and functional differentiation of chromosomes. Although the hermaphrodite chordate, Ciona intestinalis, has two self-incompatibility genes, this incompatibility system is incomplete and self-fertilization occurs under laboratory conditions. Here, we ...
Buechel Séverine D SD Department of Ecology and Evolution, University of Lausanne, Sorge, Le Biophore, CH-1015, Lausanne, Switzerland; Institute of Integrative Biology (IBZ), ETH Zürich, Universitätsstrasse 16, CH-8092, Zürich, - - 2014
Intraspecific variation in social organization is common, yet the underlying causes are rarely known. An exception is the fire ant Solenopsis invicta in which the existence of two distinct forms of social colony organization is under the control of the two variants of a pair of social chromosomes, SB and ...
Weng Katherine A KA Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of - - 2014
Chromosome segregation errors in human oocytes are the leading cause of birth defects, and the risk of aneuploid pregnancy increases dramatically as women age. Accurate segregation demands that sister chromatid cohesion remain intact for decades in human oocytes, and gradual loss of the original cohesive linkages established in fetal oocytes ...
Al-Jaru Ayman A Molecular Biology and Genetics Laboratory, Central Veterinary Research Laboratory, Dubai, United Arab - - 2014
Chromosome configurations and chiasma frequency during the metaphase I stage of spermatogenesis in the male horse are characterized in this work. The genome-wide frequency and distribution of chiasmata was detected as 49.45 ± 2.07 for 14 fertile stallions. All X and Y chromosomes shared a single chiasma at their pseudoautosomal ...
Wilkinson Gerald S GS University of Maryland; Wissenschaftskolleg zu Berlin; - - 2014
We use three allopatric populations of the stalk-eyed fly Teleopsis dalmanni from Southeast Asia to test two predictions made by the sex chromosome drive hypothesis for Haldane's rule. The first is that modifiers that suppress or enhance drive should evolve rapidly and independently in isolated populations. The second is that ...
Qin Qinbo - - 2014
The establishment of the tetraploid organism is difficult, however, is useful in genetics and breeding. In the present study, we have artificially established an autotetraploid fish line (F2-F8) derived from the distant hybridization of Carassius auratus red var. (RR, 2n=100) (female) × Megalobrama amblycephala (BB, 2n=48) (male). The autotetraploid line ...
Wells Dagan D Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, University of Oxford, Oxford, - - 2014
The majority of human embryos created using in vitro fertilisation (IVF) techniques are aneuploid. Comprehensive chromosome screening methods, applicable to single cells biopsied from preimplantation embryos, allow reliable identification and transfer of euploid embryos. Recently, randomised trials using such methods have indicated that aneuploidy screening improves IVF success rates. However, ...
Juchno Dorota D Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego 5 St., 10-718, Olsztyn, Poland, - - 2014
Crosses between 21 triploid hybrid Cobitis females and 19 C. taenia (2n = 48) males led to viable progeny; whereas no embryonic development was observed in crosses with tetraploid males (4n = 98). The ploidy status of 491 progenies randomly selected with flow cytometry (316) or chromosome analysis (175) revealed an average of 55.2 % ...
Molina-Luzón Maria Jesús MJ Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain, - - 2014
In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems related with sexual maturation, and ...
Hornak Miroslav M Central European Institute of Technology - Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic; Repromeda, Centre for Assisted Reproduction, Vinicni 235, 615 00 Brno, Czech Republic. Electronic address: - - 2014
Complex chromosomal rearrangements (CCR) represent rare structural chromosome abnormalities frequently associated with infertility. In this study, meiotic segregation in spermatozoa of an infertile normospermic carrier of a 4-breakpoint t(1;3;6) CCR was analysed. A newly developed array comparative genomic hybridization protocol was used, and all chromosomes in 50 single sperm cells ...
Schmid Michael M Department of Human Genetics, Biocenter, University of Würzburg, 97074, Würzburg, - - 2014
Chromosome aberrations (aneuploidies mostly) are the cause of the majority of spontaneous abortions in humans. However, little is known about defects in the underlying molecular mechanisms resulting in chromosome aberrations and following failure of preimplantation embryo development, initiation of implantation and postimplantation pregnancy loss. We suggest that defects of the ...
Kumar S - - 2014
The present paper deals with cytological studies on 31 populations covering 17 species belonging to 10 genera of Apiaceae from Western Himalayas. The chromosome numbers in the two species as Chaerophyllum capnoides (n = 11) and Heracleum brunonis (n = 11), along with additional cytotypes for Pimpinella acuminata (n = ...
Salimi Mahdieh M Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, - - 2014
Genome instability is a main cause of chromosomal alterations in both somatic and germ cells when exposed to environmental, physical and chemical genotoxicants. Germ cells especially spermatozoa are more vulnerable to suffering from DNA damaging agents during spermatogenesis and also more potent in transmitting genome instability to next generation. To ...
Wang Li - - 2014
Chromosome aneuploidies commonly arise in embryos produced by assisted reproductive technologies and represent a major cause of implantation failure and miscarriage. Currently, pre-implantation genetic diagnosis (PGD) is performed by array-based methods to identify euploid embryos for transfer to the patient. We speculated that a combination of next generation sequencing technologies ...
Cheng Hsin-Hsin HH Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 123, Ta-Pei Road, Niao-Sung Dist., Kaohsiung, 833, Taiwan, - - 2014
The objective of this study is to compare the chromosomal distribution of early miscarriages with or without embryonic poles. It was a retrospective study of 223 women who underwent dilation and curettage (D&C) between 1995 and 2013 for early miscarriages. The presence or absence of a fetal pole was evaluated ...
Vega Mario M Department of Obstetrics and Gynecology, Mount Sinai St. Luke's-Roosevelt, Icahn School of Medicine at Mount Sinai, New York. Electronic address: - - 2014
To test the hypothesis that the blastulation rate is higher in euploid embryos than in aneuploid embryos as assessed by cleavage-stage biopsy with array-comprehensive genomic hybridization (aCGH). Retrospective cohort study. University-affiliated institution. Forty-one patients with 48 in vitro fertilization (IVF) cycles and 385 embryos that underwent cleavage-stage preimplantation genetic screening (PGS) ...
Kilani Z Z The Farah Hospital, Amman, - - 2014
This study included 173 young couples of proven fertility who had previously undergone preimplantation genetic screening for chromosomes X and Y for family balancing. Several months later, when the outcome of the pregnancies was already known, the blastomeres from the corresponding embryos transferred were reanalysed by fluorescence in-situ hybridization (FISH) ...
Tobler Kyle J KJ Department of Gynecology and Obstetrics, Division of Reproductive Endocrinology and Infertility, Johns Hopkins Medical Institutions, Phipps 264, 600 N. Wolfe Street, 21287, Baltimore, MD, - - 2014
To compare single nucleotide polymorphism (SNP) and comparative genomic hybridization (aCGH) microarray platforms to evaluate embryos for parental translocation imbalances and aneuploidy. A retrospective review of preimplantation genetic diagnosis and screening (PGD/PGS) results of 498 embryos from 63 couples undergoing 75 in vitro fertilization cycles due to parental carriers of ...
He Yajun Y Cotton Molecular Breeding Laboratory, University of Georgia, Tifton, GA, 31793, - - 2014
We report a second major QTL for root-knot nematode resistance in the highly resistant Upland cotton line M-120RNR and show epistasis between two resistant QTLs with different mechanisms conferring resistance. In an earlier study, we identified a major QTL on Chromosome 11 associated with resistance to root-knot nematode in the ...
Du X Z XZ College of Life Science, Hubei University, Wuhan, - - 2014
In this paper, interspecific crosses among Crambe abyssinica, Crambe hispanica, and Crambe kralikii were reported. In the C. hispanica x C. abyssinica (H x A) cross, 118 F1 hybrids were produced without embryo rescue, while 5 F1 hybrids were obtained with embryo rescue, when C. hispanica was used as the ...
Liu Dandan D State Key Laboratory of Reproductive Biology; Institute of Zoology; Chinese Academy of Sciences; Beijing, China; University of Chinese Academy of Sciences; Beijing, China; Ottawa Hospital Research Institute; The Ottawa Hospital - General Campus; Ottawa, Ontario, - - 2014
The spindle assembly checkpoint, SAC, is a surveillance mechanism to control the onset of anaphase during cell division. SAC prevents anaphase initiation until all chromosome pairs have achieved bipolar attachment and aligned at the metaphase plate of the spindle. In doing so, SAC is thought to be the key mechanism ...
McPherson Marla C MC Department of Animal Science, University of California, One Shields Ave, Davis, CA, 95616, - - 2014
Well-characterized molecular and cytogenetic maps are yet to be established in Japanese quail (Coturnix japonica). The aim of the current study was to cytogenetically map and determine linkage of specific genes and gene complexes in Japanese quail through the use of chicken (Gallus gallus) and turkey (Meleagris gallopavo) genomic DNA ...
Moradian Hasan H Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, PB 76169-133, Kerman, Iran, - - 2014
The purpose of this study was to identify genomic regions, quantitative trait loci (QTL), affecting carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, two white and wild strains of Japanese quail (16 birds) were crossed reciprocally and F1 generation (34 birds) was created. ...
Jurewicz Joanna J Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, Lodz, Poland. Electronic address: - - 2014
Different environmental and lifestyle factors may interfere with the normal disjunction of sister chromatids/chromosomes during meiosis and may cause aneuploidy. The aim of the study was to examine the association between lifestyle factors and sperm aneuploidy. The study population consisted of 212 healthy men under 45 years of age attending ...
Chen Chun-Kai CK Department of Obstetrics and Gynecology, Chang-Gung Memorial Hospital and Medical College, TaoYuan, - - 2014
The presence of reciprocal and Robertsonian chromosomal rearrangement is often related to recurrent miscarriage. Using preimplantation genetic diagnosis, the abortion rate can be decreased. Cases treated at our center were reviewed. A retrospective analysis for either Robertsonian or reciprocal translocations was performed on all completed cycles of preimplantation genetic diagnosis ...
Skinner Benjamin M BM School of Biosciences, University of Kent, Canterbury, UK, CT2 - - 2014
There is a growing interest in copy number variation (CNV) and the recognition of its importance in phenotype, disease, adaptation and speciation. CNV data is usually ascertained by array-CGH within-species, but similar inter-species comparisons have also been made in primates, mice and domestic mammals. Here, we conducted a broad appraisal ...
Kaser Daniel J DJ Division of Reproductive Medicine, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, - - 2014
Since report of the first live birth following preimplantation genetic screening (PGS) in 1995, the procedure and available technologies for aneuploidy detection have rapidly evolved. Through these efforts, the biology of meiotic and mitotic segregation errors has been partially elucidated. A process that began with polar body biopsy and four-color ...
Ohnesorg T T Murdoch Children's Research Institute and Department of Paediatrics, The University of Melbourne, Royal Children's Hospital, Melbourne, Vic., - - 2014
One of the defining events during human embryonic development with the most far-reaching effects for the individual is whether the embryo develops as male or female. The crucial step in this process is the differentiation of the bipotential embryonic gonads into either testes or ovaries. If the embryo inherits X ...
Hook Ernest B EB School of Public Health, University of California, Berkeley, CA, USA, - - 2014
We review the data pertinent to the hypothesis we proposed three decades ago, that all embryos that survive gestation as women with Turner syndrome and have an ostensibly non-mosaic 45,X karyotype, actually are cryptic mosaics for a "rescue line" that includes a viable karyotype. Reanalysis of the prevalence and frequency ...
Mania Anastasia A UCL Centre for PGD, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, United Kingdom; Centre for Reproductive and Genetic Health (CRGH), The New Wing Eastman Dental Hospital, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Electronic address: - - 2014
This is a retrospective study aiming to assess telomere length in human embryos 4days post fertilization and to determine whether it is correlated to chromosomal ploidy, embryo developmental rate and patient age. Embryos were donated from patients undergoing treatment in the assisted conception unit. Seven couples took part, generating 35 ...
Basile Natalia N IVI Madrid, Madrid, Spain. Electronic address: - - 2014
To study the differences in the cleavage time between chromosomally normal and abnormal embryos and to elaborate an algorithm to increase the probability of noninvasively selecting chromosomally normal embryos. Retrospective cohort study. University-affiliated infertility center. Preimplantation genetic screening patients (n = 125; n = 77 with ET), including cases of ...
Ori R Jabbari RJ Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, PB 76169-133, Kerman, - - 2014
The Japanese quail (Coturnix japonica) is an important agricultural species and is an animal model for genetic researches. This study was conducted to identify quantitative trait loci (QTL) affecting live weight and growth rate on chromosome 3 in quail. Two strains of Japanese quail including wild and white were crossed ...
Yun Yan Y Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, - - 2014
As women get older their oocytes become susceptible to chromosome mis-segregation. This generates aneuploid embryos, leading to increased infertility and birth defects. Here we examined the provenance of aneuploidy by tracking chromosomes and their kinetochores in oocytes from young and aged mice. Changes consistent with chromosome cohesion deterioration were found ...
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