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Wu Elizabeth X EX Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, British Columbia, - - 2014
To investigate X-chromosome inactivation (XCI) skewing in female newborns conceived by intracytoplasmic sperm injection (ICSI), in vitro fertilization (IVF), and naturally. Case-control study. Research institution. A total of 185 female newborns, including 60 conceived by intracytoplasmic sperm injection (ICSI), 73 by in vitro fertilization (IVF), and 52 naturally conceived (NC). DNA was ...
Azevedo Ana Raquel AR 1Department of Microscopy, Laboratory of Cell Biology, Multidisciplinary Unit for Biomedical Research-UMIB, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira, Porto, - - 2014
The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at ...
Zheng Hong-Yun HY Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of - - 2014
To determine the frequencies and the characteristics of Y chromosome microdeletions (pl) in infertile men from central China to perform appropriate therapeutic choices by updated multiplex-PCR. In this study, we established a novel universal primer-multiplex-PCR (U-M-PCR) method to overcome the disadvantages of traditional multiplex PCR (M-PCR). We chose 15 sequence-tagged ...
Mierla Dana D Life Memorial Hospital, Bucharest, Romania ; Department of Genetics, Faculty of Biology, University of Bucharest, Bucharest, - - 2014
Chromosomal abnormalities and Y chromosome microdeletions are regarded as two most frequent genetic causes associated with failure of spermatogenesis in the Caucasian population. To investigate the distribution of genetic defects in the Romanian population with azoospermia or severe oligozoospermia, karyotype analysis by G-banding was carried out in 850 idiopathic infertile ...
Younan D D Clinical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, - - 2014
Chromosome anomalies were suggested to be more frequent in infertile males so our case-control study aimed at evaluating the incidence of spermatic aneuploidies in forty males with severe oligoasthenoteratozoospermia (OAT) and comparing it with that in another forty males having normal semen parameters. Semen samples were collected and analysed in ...
Youssef Helmy M HM Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, - - 2014
The recessive labile locus mapped on chromosome 5HL causes irregular spikelet fertility and controls floret development as well as row-type in barley. The labile-barley displays a variable number of fertile spikelets at each rachis internode (0-3 fertile spikelets/rachis internode) which is intermediate between that observed in two- or six-rowed types. Previous re-sequencing ...
Hussein A A AA Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, - - 2014
Azoospermia factor region (AZF) deletions (AZFa, AZFb, AZFc and AZFd) in the Y chromosome were analysed in male infertility subjects in various populations with conflicting results. This study comprised of 54 infertile males and 63 fertile controls, and the frequency of AZFa, AZFb, AZFc and AZFd deletions were determined using ...
Campbell Polly P Oklahoma State University; University of - - 2014
The genetic basis of hybrid male sterility in house mice is complex, highly polygenic, and strongly X-linked. Previous work suggested that there might be interactions between the Mus musculus musculus X and the Mus musculus domesticus Y with a large negative effect on sperm head morphology in hybrid males with ...
Turner Leslie M LM Max Planck Institute for Evolutionary Biology, Evolutionary Genetics, Ploen, Germany ; University of Wisconsin, Laboratory of Genetics, Madison, Wisconsin, United States of - - 2014
Hybrid dysfunction, a common feature of reproductive barriers between species, is often caused by negative epistasis between loci ("Dobzhansky-Muller incompatibilities"). The nature and complexity of hybrid incompatibilities remain poorly understood because identifying interacting loci that affect complex phenotypes is difficult. With subspecies in the early stages of speciation, an array ...
Morán Tomás T the Grup de Biologia Evolutiva, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, - - 2014
To date, different studies about the genetic basis of hybrid male sterility (HMS), a postzygotic reproductive barrier thoroughly investigated using Drosophila species, have demonstrated that no single major gene can produce hybrid sterility without the cooperation of several genetic factors. Early work using hybrids between Drosophila koepferae (Dk) and Drosophila ...
Mauro Marco M Novaetech S.r.l., Via J. F. Kennedy n.5, 80125, Napoli, Italy. - - 2014
Flow cytometry is to date the only commercially viable technique for sex preselection of mammalian spermatozoa, measuring the different DNA content in X- and Y-chromosome bearing spermatozoa. Here we present experimental evidence of a measurable difference between bovine spermatozoa bearing X- and Y-chromosomes based on their buoyant mass. Single cells ...
Pylyp L L Laboratory of Cytogenetics, Clinic of Reproductive Medicine 'Nadiya', Kiev, - - 2014
Balanced chromosomal translocations do not normally have phenotypic manifestation, but lead to increased risk of infertility, miscarriage and live-birth of chromosomally unbalanced offspring in carriers. The risk assessment of such outcomes in carriers of rare chromosomal abnormalities is complicated since limited information is available on the frequencies of unbalanced sperm ...
Martorell M R MR Unitat de Reproducció Humana i Diagnòstic Genètic, Clínica Girona, Girona, - - 2014
Two fragile sites, FRA16B and FRA16C, are located in the chromosome band 16q22.1. Neither of them is associated with any specific clinical condition. We report the development and outcome of a clinically applied PGD cycle in a couple who had difficulty in achieving pregnancy. The woman was a carrier of ...
Havelka Milo - - 2014
Evolution of sturgeons and paddlefishes (order Acipenseriformes) is inherently connected with polyploidization events which resulted in differentiation of ploidy levels and chromosome numbers of present acipenseriform species. Moreover, allopolyploidization as well as autopolyploidization seems to be an ongoing process in these fishes and individuals with abnormal ploidy levels were occasionally ...
Lyons Russell E - - 2014
Previous genome-wide association studies have identified significant regions of the X chromosome associated with reproductive traits in two Bos indicus-influenced breeds: Brahman cattle and Tropical Composites. Two QTL regions on this chromosome were identified in both breeds as strongly associated with scrotal circumference measurements, a reproductive trait previously shown to ...
Chinone Ayako A Department of Biosciences and Informatics, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522, - - 2014
Although polyploids are common among plants and some animals, polyploidization often causes reproductive failure. Triploids, in particular, are characterized by the problems of chromosomal pairing and segregation during meiosis, which may cause aneuploid gametes and results in sterility. Thus, they are generally considered to reproduce only asexually. In the case ...
Piomboni Paola - - 2014
Chromosomal abnormalities are relevant causes of human infertility, affecting 2 -14 % of infertile males. Patients with seminal anomalies could be affected by improper meiotic recombination and increased sperm chromosome aneuploidy. Since the transmission of a haploid chromosomal asset is fundamental for embryo vitality and development, the study of sperm ...
Utsunomiya Yuri T YT Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Faculdade de Ciências Agrárias e Veterinárias, UNESP - Univ Estadual Paulista, Jaboticabal, São Paulo, - - 2014
The reproductive performance of bulls has a high impact on the beef cattle industry. Scrotal circumference (SC) is the most recorded reproductive trait in beef herds, and is used as a major selection criterion to improve precocity and fertility. The characterization of genomic regions affecting SC can contribute to the ...
Rouen Alexandre A Medical Genetics and Embryology Department, AP-HP, Trousseau Hospital, 28 avenue du Docteur Arnold Netter, 75012 Paris, - - 2014
Introduction. Balanced chromosomal carriers, though usually healthy, are confronted with recurrent spontaneous abortions and malformations in the offspring. Those are related to the transmission of an abnormal, chromosomally unbalanced genotype. We evidenced that the proportion of unbalanced spermatozoa can be significantly decreased through a sperm preparation process called discontinuous gradient ...
Wiland Ewa - - 2014
Whole arm t(9;13)(p11;p12) translocations are rare and have been described only a few times; all of the previously reported cases were familial. We present here an infertile male carrier with a whole-arm reciprocal translocation dic(9;13)(p11.2;p12) revealed by GTG-, C-, and NOR-banding karyotypes with no mature sperm cells in his ejaculate. ...
Khabour Omar F OF Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology Irbid 22110, - - 2014
The azoospermia factor (AZF) region of the human Y chromosome contains essential genes for spermatogenesis. Microdeletions in AZF region has been shown to cause male infertility. The aim of this investigation was to determine the frequency of AZF microdeletions in Jordanian infertile males. A sample of 100 infertile males (36 ...
Gustafsson A Lovisa S AL National Centre for Biosystematics, Natural History Museum, University of Oslo, Oslo, - - 2014
Crossing experiments indicate that hybrid sterility barriers frequently have developed within diploid, circumpolar plant species of the genus Draba. To gain insight into the rapid evolution of postzygotic reproductive isolation in this system, we augmented the linkage map of one of these species, D. nivalis, and searched for quantitative trait ...
Olszewska Marta M Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, - - 2014
Complex chromosome rearrangements (CCRs) are structurally balanced or unbalanced aberrations involving more than two breakpoints on two or more chromosomes. CCRs can be a potential reason for genomic imbalance in gametes, which leads to a drastic reduction in fertility. In this study, the meiotic segregation pattern, aneuploidy of seven chromosomes ...
Edwards Amy M AM School of Zoology, University of Tasmania, Private Bag 5, Hobart, Tasmania 7001, - - 2013
Sex allocation research in mammals has focussed almost exclusively on mothers under the assumption that the male contribution is genetically determined during meiosis and therefore not under adaptive control. Although early studies on sperm traits suggested that sex ratios were at parity, technological advances have made analysis more reliable and ...
Suganthi Ramaswamy R Department of Biotechnology, Dr.G.R. Damodaran College of Science, Coimbatore, - - 2014
Spermatogenesis is an essential stage in human male gamete development, which is regulated by many Y chromosome specific genes. Most of these genes are centred in a specific region located on the long arm of the human Y chromosome known as the azoospermia factor region (AZF). Deletion events are common ...
Young Heather A - - 2013
The role of environmental pesticide exposures, such as pyrethroids, and their relationship to sperm abnormalities are not well understood. This study investigated whether environmental exposure to pyrethroids was associated with altered frequency of sperm sex chromosome disomy in adult men. A sample of 75 subjects recruited through a Massachusetts infertility ...
Benkhalifa Moncef - - 2013
Male factors account for approximately 50% of reproductive pathology. Different disorders, including urogenital and endocrine system development abnormalities, lead to testicular and gametogenesis defects. Parallely, studies have reported that somatic and germ cell genome decay are a major cause of male infertility. It has been shown that in somatic karyotype, ...
Mohammadi Reza R Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO, Box 7777, Metro Manila, Philippines. - - 2013
Salinity tolerance in rice is critical at reproductive stage because it ultimately determines grain yield. An F2 mapping population derived from a Sadri/FL478 cross was exposed to saline field conditions (6-8 dS m(-1)) after the active tillering stage to identify reproductive stage specific QTLs for salinity tolerance. Genetic linkage map ...
Azizeddin A A Department of Biomedical Sciences, University of Guelph, Guelph, ON, - - 2014
With the advancement of assisted reproductive biotechnologies, preselecting the sex of offspring has become an important goal for cattle and other livestock breeding as well as for research. The aim of this study was to investigate the feasibility of producing enriched pools of X- or Y-chromosome-bearing sperm by vertical swim-up ...
Wünsch Lisa K - - 2013
Hybrid male sterility is a common reproductive isolating barrier between species. Yet, little is known about the actual developmental causes of this phenomenon, especially in naturally hybridizing species. We sought to evaluate the developmental causes of hybrid male sterility, using spadefoot toads as our study system. Plains spadefoot toads (Spea ...
Bosseboeuf Adrien - - 2013
In dogfish, spermatogenesis progresses from a restricted germinative zone which lines the dorsal testicular vessel. Single spermatogonia (As), including the spermatogonial stem cells (SSCs), produce successively paired (Ap), undifferentiated (Au4 to Au512), differentiated spermatogonia (Ad1 to Ad8), and preleptotene spermatocytes (PL) through 13 mitoses. Dogfish spermatogonial subpopulations present classical morphological ...
Kociucka B - - 2013
Reciprocal translocations pose a serious problem in pig breeding due to the reduced fertility of the carriers. This paper presents a new reciprocal translocation in a phenotypically normal, but hypoprolific (20% reduction) boar. Chromosome banding as well as the FISH technique with the use of BAC and telomeric probes was ...
Vozdova M - - 2013
Globozoospermia, characterised by the presence of round spermatozoa lacking acrosomes in an ejaculate, is a known cause of male infertility. Semen analysis, including sperm chromatin structure assay, toluidine blue, chromomycin A3 and aniline blue staining and fluorescence in situ hybridisation, was performed in an infertile globozoospermic patient to establish to ...
Medarde Nuria - - 2013
Modularity is an important feature in the evolvability of organisms, since it allows the occurrence of complex adaptations at every single level of biological systems. While at the cellular level the modular organization of molecular interactions has been analyzed in detail, the phenotypic modularity (or variational modularity) of cell shape ...
Siqueira Sérgio S Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil . ; Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Jequié, BA, Brazil - - 2013
The current taxonomy of most Atelopus species is based on morphological and color data only. Recent studies suggest that A. spumarius may represent a species complex assigned under the same name. Karyotypic data and description of sperm ultrastructure for 13 specimens of A. spumarius are presented here for the first ...
Godo Anna - - 2013
To find out the meiotic segregation behaviour of the t(1;8;2)(q42;p21;p15), to evaluate the occurrence of interchromosomal effects, and to determine whether there is an accumulation of unbalanced products in aneuploid/diploid gametes. A sequential FISH protocol based on two successive hybridization rounds over the same spermatozoa was performed to determine the ...
Yasin S R - - 2013
The long arm of the Y chromosome contains nonoverlapping regions termed azoospermia factor (AZF) with great influence on male fertility. Microdeletions at these regions minimise the males' ability to father offsprings. In this preliminary study, we attempted to screen the presence or absence of twenty Y chromosome's sequence-tagged sites (STS) ...
Orsztynowicz M - - 2013
The standard procedure of artificial insemination with fresh equine spermatozoa involves short-term storage (to 48 h at 5°C). This procedure is accompanied by a gradual loss of sperm viability. The aim of this study was to investigate whether the X/Y ratio of equine spermatozoa is affected by short-term storage and the ...
Park Se Hwan - - 2013
We assessed the frequency of azoospermia factor a (AZFa), AZFb, and AZFc deletions and examined correlations between the deletion sites and the success rates of sperm presence within the ejaculate and surgical sperm retrieval in Korean men. A total of 1,919 azoospermic and severely oligozoospermic men were assessed for Y ...
Pylyp Larysa Y - - 2013
To study meiotic segregation patterns of Robertsonian translocations in sperm of male carriers and to assess the frequencies of unbalanced sperm formation. FISH with combination of probes to detect all the variants of meiotic segregation was performed on decondensed sperm nuclei of 5 carriers of der(13;14), 3 carriers of der(14;21) ...
Akbari Omar S - - 2013
A widespread phenomenon in nature is sex ratio distortion of arthropod populations caused by microbial and genetic parasites. Currently little is known about how these agents alter host developmental processes in order to favor one sex or the other. The Paternal Sex Ratio (PSR) chromosome is a non-essential, paternally transmitted ...
Olszewska Marta - - 2013
The presence of reciprocal chromosome translocations (RCTs), as well as sperm chromatin disturbances, is known to exert negative influence on male fertility. The aim of this study was to identify an association between chromosome structural rearrangements in male RCT carriers and sperm seminological parameters (concentration, motility, morphology), chromatin status (fragmentation ...
Fortes Marina R S - - 2013
A current challenge in genetic improvement of cattle is to identify genomic selection strategies that could work across breeds. Breed differences, scarcity of data, and lack of quantitative trait loci (QTL) validation contribute to this challenge. We conducted a review of the literature to identify QTL, markers, and candidate genes ...
Sakr Saber Abdelruhman - - 2013
To evaluate the ameliorative role of grapefruit juice on the cytogenetic and testicular damage induced by the antiarrythmic drug amiodarone in albino rats. Animals were divided into four groups. Group I was considered as control. Group II was given grapefruit juice at a dose level of 27 mL/kg body weight. ...
Farah Omar Ibrahim - - 2013
Sperm is produced by the testis and mature in the epididymis. For having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. The effects of genetic and environmental factors result in sperm vulnerability to damage in the process of ...
Zhang Changjun C The Reproductive Research Center, Renmin Hospital, Hubei university of Medicine, Shiyan, - - 2013
A 24-year-old man was diagnosed with klinefelter's syndrome (KS) and his wife was found to have an inversion on chromosome 9-46, XX, inv (9) (p11q21)- because of infertility. Intracytoplasmic sperm injection (ICSI) was performed for fertilization after fluorescence in-situ hybridization (FISH) was used to analyze the aneuploidy rate of the ...
Levron Jacob - - 2013
Eight infertile men with various degrees of oligoasthenoteratozoospermia and repeated implantation failure were selected for this study due to exceptionally high rates of sperm aneupoidy in their ejaculates. All subjects had normal physical examination, karyotype and serum FSH concentration. Prior to IVF treatment, spermatozoa was collected, processed, micromanipulated and tested ...
Stenberg P - - 2013
We review the different modes of meiosis and its deviations encountered in polyploid animals. Bisexual reproduction involving normal meiosis occurs in some allopolyploid frogs with variable degrees of polyploidy. Aberrant modes of bisexual reproduction include gynogenesis, where a sperm stimulates the egg to develop. The sperm may enter the egg ...
Zhang Yong-Sheng - - 2013
OBJECTIVE: To determine the frequencies and the characteristics of Y chromosome microdeletion in infertile men from northeastern China to perform appropriate therapeutic choices. MATERIALS AND METHODS: The study included 1738 infertile men. Sperm concentration was measured according to standard methods and karyotype analysis was performed on peripheral blood lymphocytes with ...
Zaimy Mohammad Ali MA Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ; Department of Genetics, Faculty of Medical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, - - 2013
Background: About 15% of couples have infertility problems which 40% of them are related to the male factors. Genetic factors are candidate for about 10% of male infertility conditions. Among these, AZFa, AZFb, AZFc and AZFd regions on the Yq are considered most important for spermatogenesis. Microdeletions of these regions ...
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