A high-performance liquid chromatographic method was optimized and validated for the determination of desacetyl nitazoxanide (tizoxanide), the main active metabolite of nitazoxanide in human plasma, urine and breast milk. The proposed method used a CN column with mobile phase consisting of acetonitrile-12mM ammonium acetate-diethylamine in the ratio of 30:70:0.1 (v/v/v) ...

A series of polymeric ionic liquids (PILs) possessing varied chemical makeup and composition were applied as selective solid-phase microextraction (SPME) sorbent coatings for the analysis of genotoxic impurities (GTIs) and related structurally alerting compounds, namely, alkyl halides and aromatics. In addition to exploiting two previously synthesized PILs as selective coatings, ...

A molecularly imprinted polymer with trinitrotoluene as the template molecule was synthesized and used as the novel coating for solid-phase microextraction of the nitroaromatic explosive 2,4,6-trinitrotoluene for its selective determination. The fiber was characterized in terms of coating thickness, morphology, intra- and inter-batch repeatability and extraction efficiency. An average thickness ...

Preparations formulated as coated or film-coated tablets, containing levonorgestrel and the combination ethinylestradiol/levonorgestrel, were evaluated in a comparative study. This study comprised in vitro dissolution, assay and content uniformity. The analytical methods were previously validated according to international guidelines. All examined products complied with the postulated requirements.

We demonstrated a conformal phosphor coating method for phosphor-converted white light-emitting diodes (LEDs) using capillary microchannel formed by a fixture. The mixture of the phosphor particles and the silicone spontaneously flows in capillary microchannel and was pinned at the edge of the fixture due to the surface tension effect. The ...

Metabolomic studies by NMR spectroscopy are increasingly employed for a variety of biomedical applications. A very standardized 1D proton NMR protocol is generally employed for data acquisition, associated with multivariate statistical tests. Even if targeted approaches have been proposed to quantify metabolites from such experiments, quantification is often made difficult ...

Zinc oxide (ZnO) nanorods based solid-phase microextraction (SPME) coating was directly prepared on stainless steel wires using in situ hydrothermal growth method. This coating has high surface-to-volume ratio with a diameter in the range of 300-500nm and a thickness of about 3-5μm. A guiding tube was introduced into the laboratory-made ...

This case report involves four dairies in the Willamette Valley, Oregon, which experienced reproductive problems associated with the presence of a large, previously unidentified, peak eluting at 5 min in a standard ergovaline high-performance liquid chromatography assay of perennial ryegrass silage fed to those animals. Mycotoxin analysis of the silage was ...

Leaves of Palicourea marcgravii were extracted successively with hexane, ethyl acetate and ethanol in order to evaluate their acaricidal activity on larvae and adult stages of the cattle tick Rhipicephalus (Boophilus) microplus. The ethyl acetate extract showed the highest bioactivity of the tested extracts, which contained 0.12% monofluoroacetic acid. On ...

Data on the occurrence of T-2 and HT-2 toxins in commodities and feed in Croatia and in Europe are very limited. In the present study, 50 feed and commodity samples tested for the presence of these mycotoxins yielded highest concentrations in cattle feed (67.68 ng/g) and oat samples (32.94 ng/g). In the ...

Abstract Leptospirosis is an important bacterial zoonotic disease globally and one of the notifiable diseases in Sri Lanka. Other than human leptospirosis, little information is available on leptospirosis in domestic and feral animals in Sri Lanka. Thus, this study attempted to determine the prevalence and carrier status of leptospirosis in ...

A square-wave anodic-stripping voltammetric method for the analysis of lead and cadmium in chicken muscle and liver was developed and validated, and the results of a monitoring study relative to chicken and pigeon meat are reported. The voltammetric method allows the analysis of lead and cadmium at the same time ...

Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five ...

Microbial activity acts as primer in the self combustion process of solid recovered fuels (SRF) during their storage or transport. Thus, EU gave mandate to the European Committee for Standardization (CEN) to develop biological methods, (i.e. respirometric method) able to assess the risk of potential self combustion of SRF. Real ...

A new axis-marking method, the mapping method, for toric intraocular lens (IOL) implantation is described, and its accuracy is compared with that of conventional methods. For toric IOL implantation, the steepest corneal axis was determined by IOLMaster optical biometer measurement (Carl Zeiss Meditec). Three marking methods for accurate alignment of ...

The erythrocyte sedimentation rate (ESR) is still a widely used parameter for acute phase inflammation. Recently, new methods based on direct undiluted measurement of ESR in a standard EDTA tube have been developed. We evaluated the analytic performance of one of these new methods, the Ves-Matic Cube 200 (Diesse Diagnostica ...

Several methods of flip angle mapping for magnetic resonance imaging have been proposed. We evaluated the accuracy of five methods of flip angle measurement in the presence of measurement noise. Our analysis was performed in a closed form by propagation of probability density functions (PDFs). The flip angle mapping methods ...

An efficient one-pot method for bromination-elimination of allyl alcohol derivatives and sequential Sonogashira coupling has been developed. A highlight of the method is chemoselective DBU-promoted elimination of vicinal dibromoalkanes having an adjacent O-functional group.

The authors have indicated no significant interest with commercial supporters.

AIMS AND OBJECTIVES: This study was designed to determine the effectiveness of the auscultatory and pH methods in predicting feeding tube location in critically ill patients. BACKGROUND: There is confusion about how nurses should asses feeding tubes location at the bedside. The most common method for determining tube location is ...

The purpose of this study was to evaluate and validate an offline, automated scalp EEG-based seizure detection system and to compare its performance to commercially available seizure detection software. The test seizure detection system, IdentEvent™, was developed to enhance the efficiency of post-hoc long-term EEG review in epilepsy monitoring units. ...

Several methods are used to measure lumbar lordosis. In adult scoliosis patients, the measurement is difficult due to degenerative changes in the vertebral endplate as well as the coronal and sagittal deformity. We did the observational study with three examiners to determine the reliability of six methods for measuring the ...

BACKGROUND: The cytotoxicity of dermal substitutes may be increased by the very processes used to deplete the cells. The present research aimed to investigate the method for monitoring the cytotoxicity of cell-free dermal substitutes using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. METHODS: The cytotoxicity of four dermal substitutes was evaluated using ...

BACKGROUND: In China, two common methods are used to detect serum glucose, glucose oxidase (GOD) and hexokinase (HK) methods. We evaluated five commercially available glucose reagent kits with the Center of Disease Control (CDC) HK reference method. METHODS: Five routine methods were compared with the HK reference method according to ...

OBJECTIVE: To use multivariate statistical analysis of EEG data in order to separate EEGs of patients with Alzheimer's disease (AD) from controls. A group of individuals with mild cognitive impairment (MCI) was evaluated using the same methodology. Additionally, the effects of scopolamine on this separation are studied. METHODS: Statistical pattern ...

Although previous intravascular ultrasound (IVUS) radiofrequency-based analysis data showed acceptable reproducibility for plaque composition, measurements are not easily obtained, particularly that of lumen contour, because of the limited IVUS resolution. The purpose of this study was to compare a new measurement method (Shin's method) and the conventional measurement method for ...

Most of the automatic seizure detection schemes reported in the literature are complex for detecting seizures that are of (a) short duration, (b) minimal amplitude evolution, or (c) non-rhythmic mixed frequency epileptic activity. We present a novel morphology-based classifier to detect epileptic seizures for intracranial EEG recording. The method characterizes ...

The aim of the study was to evaluate if pH indicator strips could be used for measurements of plaque pH acidogenicity in situ. Interproximal plaque pH was measured before and up to 60 min after a 10% sucrose rinse in 30 healthy volunteers using pH indicator strips and the microtouch ...

The Japanese Society for Medical Mycology (JSMM) method used for testing the antifungal susceptibility of yeast, the MIC end point for azole antifungal agents, is currently set at IC(80). It was recently shown, however that there is an inconsistency in the MIC value between the JSMM method and the CLSI ...

The aim of this study is to represent the corneal anterior surface by utilizing radius and height data extracted from a TMS-2N topographic system with three different mathematical approaches and to simulate the visual performance. An iteratively re-weighted bi-cubic spline method is introduced for the local representation of the corneal ...

PURPOSE: To investigate the reproducibility of 3 different tracing methods to determine a reliable method to define the proper anatomical position of the mandibular canal based on cone beam computed tomography (CBCT) data. MATERIALS AND METHODS: Five dentate and 5 edentate patients were selected at random from the CBCT database. ...

The authors propose a manual measurement method for wear in total hip arthroplasty (PowerPoint method) based on the well-known Microsoft PowerPoint software (Microsoft Corporation, Redmond, Wash). In addition, the accuracy and reproducibility of the devised method were quantified and compared with two methods previously described by Livermore and Dorr, and ...

Availability of standard, continuous electroencephalography (cEEG) monitoring in ICU is very limited, although commercially available 4-channel modules are present in many ICUs. We investigated the sensitivity of such modules compared with the more complete monitoring with a standard EEG system. Seventy patients at high risk of seizures in the medical-surgical ...

OBJECTIVE: The aim of this study was to assess methods currently used for analyzing fluoridated salt in order to identify the most useful method for this type of analysis. BASIC RESEARCH DESIGN: Seventy-five fluoridated salt samples were obtained. Samples were analyzed for fluoride content, with and without pretreatment, using direct ...

A new method for early detection of extravasations during contrast-enhanced computed tomographic procedures is evaluated. The new method uses radio frequency permittivity-based sensing to detect the onset of an extravasation with high sensitivity and specificity. There were 66 subcutaneous injections performed at the antecubital fossa in 35 volunteers to evaluate ...

The recent recognition of the correlation of the hip-knee-ankle angle (HKA) with femur-tibia angle (FTA) on a standard knee radiograph has led to the increasing inclusion of FTA assessments in OA studies due to its clinical relevance, cost effectiveness and minimal radiation exposure. Our goal was to investigate the performance ...

Three cell lysis methods-freeze-thaw, osmosis, and a chemical detergent-based method-were evaluated as sample treatment procedures for platinum metallomic studies of in vitro grown human malignant cells exposed to cisplatin. The lysis methods are relatively mild, resemble those commonly used in proteomic studies, and were selected because of the proven reactivity ...

We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within ...

This study describes the modification methods of blank cartridge firing pistols in Turkey. We have examined cases submitted to the Aydin Regional Criminal Laboratory of Turkey in 2006. In total, 95 modified pistols and 300 modified cartridges were examined. The blank cartridge firing pistols are guns which look similar to ...

INTRODUCTION: Measuring the postpacing interval (PPI) and correcting for the tachycardia cycle length (TCL) is an important entrainment response (ER). However, it may be impossible to measure PPI due to electrical noise on the mapping catheter. To overcome this problem, 2 alternative methods for the assessment of ER have been ...

Graduation date: 1996

There is a current and developing need for rapid and accurate methods of barley varietal identification which go beyond traditional morphological analysis. Methods using DNA analysis have the capacity to fulfil this role with microsatellites being the current marker of choice. The majority of barley cultivars on the National List ...

BACKGROUND: Cardiac Output (CO) is an important parameter in the diagnosis and therapy of heart diseases. Inert gas rebreathing (IGR) and continuous wave Doppler ultrasound (CWD) are among the most promising newer techniques aiming at a non-invasive, point of care measurement of CO. A direct comparison of the two methods ...

Few studies have evaluated the efficiency of methods for sampling ants, especially in regions with highly variable vegetation physiognomies such as the Cerrado region of central Brazil. Here we compared three methods to collect ground-dwelling ants: pitfall traps, sardine baits, and the Winkler litter extractor. Our aim was to determine ...

This article compares several methods for performing robust principal component analysis, two of which have not been considered in previous articles. The criterion here, unlike that of extant articles aimed at comparing methods, is how well a method maximizes a robust version of the generalized variance of the projected data. ...

Soymilk and soyacake were produced by three methods: illinois, (A), cornel (B), and traditional oriental (C). Physiochemical, microbiological, and sensory properties were evaluated. Method C had the highest soymilk yield of 57.76% followed by B with 55.17% and A 52.85%. The protein contents of soymilk were 2.8, 3.5, and 3.8 ...

STUDY DESIGN: The reliabilities of and correlations among 5 standard methods of assessing cervical sagittal alignment were evaluated. OBJECTIVE: To investigate the reliabilities of and correlations among 5 standard methods of assessing cervical sagittal alignment. SUMMARY OF BACKGROUND DATA: Although various cervical sagittal alignment assessment methods are widely used, their ...

Two sampling methods (rectoanal swabs and rectal fecal grabs) were compared for their recovery of Escherichia coli O157:H7 from feedlot cattle. Samples were collected from 144 steers four times during the finishing period by swabbing the rectoanal mucosa with cotton swabs and immediately obtaining feces from the rectum of each ...

When humans acquire a conditional discrimination and are given a novel-sample-comparison choice, they often reject a comparison known to be associated with a different sample and choose the alternative comparison by default (or by exclusion). In Experiment 1, we found that if, following matching training, we replaced both of the ...

The contraceptive method chosen is an important determinant of contraceptive discontinuation. However, method choice is endogenous to contraceptive discontinuation. Using data from the 1997 Indonesia Demographic and Health Survey, we apply a multilevel multi-process model to examine the impact of method choice on three types of contraceptive discontinuation. We confirm ...