A study was carried out with four healthy young adult men, consuming a self-selected diet, to investigate quantitative aspects of the gastrointestinal absorption, urinary excretion, and body retention of egg selenium in comparison with selenite. The approach involved simultaneous consumption of egg biologically labeled (intrinsic) with the stable isotope 74Se ...

The effect of egg yolk consumption on the composition of LDL and on the concentration of HDL subclasses was studied in healthy subjects. Six volunteers consumed a diet low in cholesterol for 10 days and then daily added 6 egg yolks to their diet for another 10 days; the experiment ...

Increasing the fat content of the diet increases the proportion of large triglyceride-rich (TGR) lipoproteins (portomicrons) in laying-hen plasma, but has no effect on the size distribution of yolk TGR-lipoproteins. Electromicrographs of the ovarian follicle walls of hens fed a high-fat diet show the presence of numerous portomicron-like particles in ...

Cardiomyopathy associated with vitamin E deficiency was diagnosed in more than 100 ruminants and primates and in 106 embryos and newly hatched chicks and ducklings. Affected bird embryos failed to pip the eggshell and died inside. Newly hatched chicks and ducklings and neonatal ruminants were weak, had difficulty standing or ...

Fertile white Leghorn chicken eggs were exposed via intravitelline injections to dosages of 5.0, 10.0, or 20 mg 1,1-bis(p-chlorophenyl)-2,2,2-trichloroethane (DDT) in olive oil prior to incubation. Control embryos received only the olive oil vehicle. Eggs were placed in a forced-draft incubator for either 5 or 12 days. Embryos were removed ...

Yolk sacs (YS) were examined by gross, histochemical, and electron microscopic techniques from incubation day 12 to 10 days of age. Weights were maximal at 22 days of incubation and declined markedly thereafter; by 7 days of age, the yolk was completely absorbed. Endodermal cells of YS epithelium were highly ...

A linear relationship (r greater than .98) was found between the level of dietary biotin and plasma and egg-yolk biotin in White Leghorn hens after feeding the experimental diets for 2 weeks. This shows high reliability of these parameters as indicators of biotin availability. When plasma and egg yolk biotin ...

Two experiments were conducted to determine the effect of dietary fiber in rations of varying nutrient density on egg yolk cholesterol, energy balance and reproductive efficiency of laying hens. Initially, four diets arranged in a Latin square design were tested: low, medium and high nutrient density diets and a control ...

The effect of precoital vaginal placement of three spermicidal formulations on postcoitally recovered vaginal and cervical spermatozoa was compared. Periovulatory stumptailed macaque monkeys were administered intravaginally 1 ml of Ramses Contraceptive Vaginal Jelly (C. Schmid Products Company, Little Fall, NJ), or a pluronic gel formulation containing 0, 0.1, or 1.0% ...

Chick embryos were grown on a rotating centrifuge for periods of 2 wk to study the effects of increased gravity on the relative growth of their otoliths and femurs. Embryos, 20-21 days of age, centrifuged at 2 g showed no difference in utricular otoconial CaCO3 weights and femur CaPO4 weights ...

An estimate has been made of the time required for a population of spermatozoa capable of fertilizing the egg to be established in the oviducts of heifers mated early in oestrus. During a general or local anaesthetic, the caudal isthmus of the oviduct was ligated and sectioned 6, 8, 10 ...

Progression of ram spermatozoa within the sheep oviduct has been studied in relation to the time of ovulation. Ewes were mated at the onset of estrus and, at increasing intervals thereafter, the oviduct isthmus was transected 1.5-2.0 cm proximal to the utero-tubal junction. The incidence of fertilization and number of ...

Laying female quail of five strains (R-1, randombred control; HP and LP, high or low concentration of egg yolk very low density lipoprotein (VLDL) precursor in laying females: HW and LW, high or low 4-week body weight) were sampled after 120 days of egg production for body weight, liver weight, ...

From a randombred control population of Coturnix coturnix japonica, strains were divergently selected for high (HE) and low (LE) 120-day egg production based on individual egg records. Based on five generations of selection, realized heritabilities for HE and LE were .063 +/- .055 and .353 +/- .020, respectively. The total ...

Polyamine contents in the regenerates were determined at various stages after amputation of the forelimbs of the adult female Xenopus laevis. Putrescine, spermidine, spermine, and sym-homospermidine were detected in all the specimens examined. Cadaverine was detected only in a limited number of samples. At 5 days after amputation of forelimbs, ...

The amount of nuclear sulphydryl and disulphide groups was determined by cytofluorometry on single mouse spermatozoa from the caput, corpus and cauda epididymidis, and the vas deferens. N-(7-Dimethylamino-4-methylcoumarinyl) maleimide was used as a specific and quantitative fluorescent reagent for thiols. The sperm nuclear content of free sulphydryl groups decreased sharply ...

The relative biological value (RBV) of iron in egg yolk was compared with that of iron in ferrous sulfate in prophylactic assays with weanling rats. Egg yolk cooked by one of three different methods was used in each experiment: (1) yolk of eggs boiled in the shell, lyophilized, and mixed ...

3,3',4,4'-Tetrachlorobiphenyl (3TCB) was injected into the yolk of embryonated hens' eggs in doses of 4, 20 or 100 micrograms/kg egg. Twenty and 100 micrograms/kg resulted in the death of all the embryos. The hatching rate of the eggs treated with 4 micrograms/kg was 60% while that of the control eggs ...

After a single dose of [14C]hexachlorobenzene (HCB) via gavage into the crop, the accumulation of [14C]HCB in female bobwhite (Colinus virginianus) tissues occurred to the greatest extent in adipose tissue followed by skin, liver, brain, heart and kidney, whole blood, and muscle. There was a general relation between increasing HCB ...

The present paper shows the effect of egg yolk on chick kidney ornithine transcarbamylase (OTC) activity. A 90% egg yolk diet resulted in an increase of about 2.5-fold in kidney OTC activity. Enzyme activity increased gradually during incubation and after hatching and then decreased in accordance with disappearance of egg ...

The contribution of subcellular organelles in egg yolk-induced fatty liver was investigated in liver tissue sections and fractionated liver homogenates. When 2.0 ml. of 12.5 per cent egg yolk were administered to Wistar male rats (body weight, 250 to 350 gm.), a fatty change occurred in the liver after a ...

The response of various strains of chickens to brooding temperatures of 32.2 C and 26.7 C was compared. Variation in response was assessed by mortality, weight gain, feed consumption and feed conversion (FCR), rectal temperature, relative yolk sac and liver weights, packed cell volume (PCV), plasma glucose, and plasma cholesterol. ...

Tri-o-tolyl phosphate (TOTP) was injected into the yolk sac of 120-h chicken embryos at concentrations equivalent to 200,100, 50, 25, or 0 (vehicle control) microgram TOTP/g egg. On d 22 of incubation, all live hatchlings were wing-banded, weighed, and housed in a brooder battery according to treatment level. Birds were ...

Egg production type chickens were given the drug Probucol in their diets to determine its effect on egg production characteristics, liver lipid metabolism, egg yolk lipid synthesis, and egg yolk cholesterol concentration. In none of the three trials conducted did Probucol feeding affect egg production or body weight. The drug ...

White Leghorn hens, housed in cages were fed commercial-type corn-soy diets containing one of three levels of 3-Nitro-10 (0, .0025, and .005%) and either of two levels of xanthophyll (high and low) supplied by yellow corn. Egg yolks were collected and the yolks analyzed for color using reflectance colorimetry. The ...

Erthrocytes from African blacks with primary hepatoma were incubated with physiological amounts (1.64 microM) of nicotinamide-14C (NM-14C) and it was found that these erythrocytes could synthesize NAD from NM. After 3-hr incubation with NM-14C, a large percentage of the 14C was found in NMN, nicotinamide riboside (NR) and NAD, but ...

1. The effect of Dieldrin on phospholipid content and composition was compared in adult frog liver and in early embryos. 2. Male frogs are more resistant to acute Dieldrin treatment than females. Total phospholipids content increases in the liver of male animals while in females decreases. 3. Early embryo development ...

The possible selection of spermatozoa for fertilization by the female genital tract was investigated using genetically homogeneous, numerically adjusted 'cervix-selected' and 'unselected' rabbit spermatozoa. Samples of spermatozoa were marked by 15 min exposure to 1.3 mg TEPA/ml and then washed for 15 min. TEPA-treated and control samples were inseminated alternately ...

In vivo, t6/t6 embryos are developmentally arrested between gestation days 5.5 (short-egg-cylinder stage) and 6.75 (long-egg-cylinder stage). In the present series of studies we used both in vivo and in vitro blastocyst delay followed by in vitro outgrowth to determine whether the t6/t6 lethality is time- or stage-specific. The results ...

The relationship between cardiovascular disturbances and the formation of hematomas in trypan blue-treated chick embryos was investigated. Hematocrits from Hamburger-Hamilton stage 15 and 16 embryos (8-10 hours after treatment at stage 13) were obtained. The hematocrit value from the treated embryos was significantly lower (P less than 0.001) than the ...

Accumulation of benzo[a]pyrene (BaP) by sexually mature flatfish gonad, its transfer to developing gametes, and its subsequent effects on developing embryos were studied. Thin-layer chromatography revealed both unmetabolized BaP and polar metabolites in the ovary, wolffian ducts, oocytes, and semen of English sole 24 h after ip injection with 200 ...

Rainbow trout eggs, yolk sac fry and juveniles were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in different concentrations for 96 h. Exposure of eggs to the lowest concentration, used in this study, 0.1 ppt (10-13 glg), resulted in a significant growth retardation for 72 days. At higher concentrations significant numbers of the ...

Ligation of the ovarian end of the oviduct was used to induce hydrosalpinges in rabbits and monkeys. This procedure resulted in an accumulation of clear serous fluid within the lumen and distention of the ampullary wall. The ampullary endosalpinx responded with a general flattening of the surface architecture, especially a ...

In a 16-day experiment involving 270 32-week-old laying hens, dehydrated turf grass (DTG) and corn gluten meal (CGM) were compared as xanthophyll sources for egg yolk pigmentation. Experimental diets were formulated to contain, 0, 5, 10, 15 and 20 mg total xanthophyll/kg diet, provided as either DTG or CGM. Neither ...

Random bred Schofield albino mice were unilaterally ovariectomized or sham-operated at 6 weeks of age and were killed 4, 8, 16 or 32 weeks later. Ovarian weights were recorded before quantitative histological analysis of the total number of oocytes per ovary, the number of follicles per size class, and the ...

Epidemiological, laboratory and experimental studies make it clear that dietary cholesterol (DC) and saturated fat, independently and together, are hypercholesterolaemic and atherogenic. Some work suggests that DC, usually given as egg yolk, is the more important, but often they are consumed together. There is a threshold, usually between 100 and ...

Comparative hypercholesterolemic properties of two dietary cholesterol sources, crystalline and egg yolk powder, were investigated by feeding 60 adult White Leghorn male chickens at various levels. High dietary levels of crystalline cholesterol had a significantly greater influence on raising the serum cholesterol concentration than an equal amount of dietary cholesterol ...

Hatching eggs from 48 and 55-week-old Small White turkey breeders were used to examine the yolk sac of these poults. Influences due to flock age, egg weight, poult sex, and a two day postplacement regimen of feeding or fasting were evaluated in a factorially designed experiment. Each variable was found ...

The effects of feeding an experimental diet consisting of 16.25% (w/w) dry powdered egg yolk and 30.8% total fat (20% from lard) was compared with an isocaloric amount of control (stock) regimen in male Hormel miniature pigs. The experimental diet was started at 30 weeks of age. The serum lipids ...

Studies were undertaken to determine the effect of inhibitors of cholesterol synthesis on deposition of cholesterol in eggs of Japanese quail. Results indicate that this bird responds similarly to the laying hen, making it a useful screening device for these types of compounds. Administration of either triparanol or 20,25 diazacholesterol ...

Two experiments were conducted using 14 mares. In Exp. 1, mares were inseminated with semen treated with TEPA, which, in other species, has been shown to lead to an arrest in ovum cleavage at 2--4 cells. The oviducts and/or uterus were then flushed 7--10 days after ovulation in 6 mares ...

In experiments to see whether, in the possible interests of human health, the polyunsaturated fatty acid (PUFA) content of the chicken's egg can be increased by nutritional means, three strains of hen, light, medium, and heavy, each at the peak of lay, were first fed a basal, commercial, low-fat diet. ...

Studies were conducted to establish the differences in yolk cholesterol concentrations in eggs from various avian species. Cholesterol was determined by a modification of the colorimetric procedure of Pearson et al. (1953). Species listed in increasing concentrations of cholesterol per gram of yolk, were guinea fowl, chicken, pheasant, quail, turkey, ...

1. It was found that the environment determined by the hen, namely protein, fat and moisture, had no effect on the growth of the embryo during the first 14 d of development. 2. The amount of protein available influenced growth during the last week of incubation but the amount of ...

140La accumulations in 20 tissues and tissue products of laying Japanese quail 1, 6 and 18 hr. after an intravenous dose of 15 mumol. La/100 g. body weight were greatest in the liver (43.1% at 18 hr.) and the growing oocytes (8.19% at 18 hr.). 47Ca accumulations 6 hr. after ...

Styrene and styrene oxide were injected into the air space of fertilized chicken eggs at different times during an incubation period of 14 days. The toxicity of styrene and styrene oxide when injected on the fourth day of incubation revealed an LD50 of 40 mumol/egg and 1.5 mumol/egg, respectively. Malformations ...

Effects of dietary lipid factors (saturated and unsaturated oil, cholesterol and plant sterols) on the serum and egg yolk cholesterol levels of the laying hen were investigated. Single Comb White Leghorn laying hens, at thirty weeks of age, were used in two trials by feeding two basal diets containing 8.0% ...

Thirty pregnant mice were undernourished by providing low protein diets (PM) or providing approximately one-third their normal diets (PCM) for 9 days from the 8th to the 16th day of gestation. Another 15 pregnant mice, which were fed a normal diet, served as the control. On the 16th day of ...

The effect of methallibure on ovary of H. fossilis was studied. Intraperitoneal administration of methalibure (100 microgram/g body weight) once a week for 4 weeks was very effective in suppressing the gametogenic and endocrine activities of ovary. The regressed condition of ovary was also apparent in GSI (2.84 to 1.09) ...

1. Lines were selected for divergence in yolk-cholesterol concentration for three and four generations in the Cornell Controll (Leghorn) and Athens-Canadian (AC) (meat-type) randombred populations respectively. Selection was based on both individual and family records. 2. Cholesterol values were obtained from two consecutively laid eggs when birds were 28 weeks ...