Search Results
Results 1 - 50 of 1144
1 2 3 4 5 6 7 8 9 10 >
Keith Scott Alexander SA Department of Pediatrics, University of Pittsburgh School of Medicine, 7129 Rangos Research Centre, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA - - 2014
A wealth of knowledge on the genetic mechanisms that govern aging has emerged from the study of mutants that exhibit enhanced longevity and exceptional resilience to adverse environmental conditions. In these studies, lifespan has been an excellent proxy for establishing the rate of aging, but it is not always correlated ...
Gautier Jean-Charles JC 1Sanofi R&D, Paris, - - 2014
Differences were examined between male and female Sprague-Dawley rats in basal levels of a wide range of urinary biomarkers, including 7 recently qualified biomarkers. The data were generated from urine samples collected on 3 occasions from untreated rats included in a study of the effect of gentamicin nephrotoxicity on urinary ...
Zhang Zheng - - 2014
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been proposed as an ideal autologous stem cell source for cell-based therapy for myocardial infarction (MI). However, decreased viability and impaired function of aged MSCs hampered the therapeutic efficacy of engrafted MSCs, and the underlying mechanisms remain unclarified. Here, we investigated the role ...
Polanowski Andrea M AM Australian Antarctic Division, 203 Channel Highway, Kingston, TAS, 7050, - - 2014
Age is a fundamental aspect of animal ecology, but is difficult to determine in many species. Humpback whales exemplify this as they have a lifespan comparable to humans, mature sexually as early as four years and have no reliable visual age indicators after their first year. Current methods for estimating ...
Bidlingmaier Martin M Endocrine Research Laboratories (M.Bid., M.Bie.), Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, 80336 Munich, Germany; Metabolic Center (N.F., H.W.), Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Germany; Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH) (R.T.E., A.D.), Institute of Epidemiology II, Neuherberg, Germany; Department of Endocrinology, Diabetes, and Nutrition (J.S., A.F.H.P., A.M.A.), Charité-University Medicine Berlin, Berlin, Germany; Experimental and Clinical Research Center (J.S.), Charité-University Medicine Berlin and Max-Delbrück Centre Berlin-Buch, Berlin, Germany; Center for Cardiovascular Research (J.S., A.M.A.), Charité-University Medicine Berlin, Berlin, Germany; Children's Clinic Randers (O.D.W.), Randers, Denmark; Göteborg Pediatric Growth Research Center (J.R.), The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden; Center for Pediatric Research (A.K.), Hospital for Children and Adolescents, Department of Women's and Child Health, University of Leipzig, Leipzig, Germany; Klinische Abteilung und Labor für Endokrinologie und Stoffwechsel (B.O.-P.), Universitätsklinik für Innere Medizin, Medizinische Universität Graz, Austria; Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe-Grosshadern (C.H.), Klinikum der Universität München, Munich, Germany; Medical Research Laboratory (J.F.), Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark; Department of Endocrinology and Internal Medicine (J.F.), Aarhus University Hospital, Aarhus, Denmark; and Department of Clinical Nutrition (A.F.H.P., A.M.A.), German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, - - 2014
Context: Measurement of IGF-1 is a cornerstone in diagnosis and monitoring of GH-related diseases, but considerable discrepancies exist between analytical methods. A recent consensus conference defined criteria for validation of IGF-1 assays and for establishment of normative data. Objectives: Our objectives were development and validation of a novel automated IGF-1 ...
Stump Donald G DG Developmental and Reproductive Toxicology, WIL Research Laboratories, LLC, Ashland, - - 2014
The male and female pubertal assays, which are included in the U.S. Environmental Protection Agency's (EPA) Endocrine Disruptor Screening Program (EDSP) Tier 1 battery, can detect endocrine-active compounds operating by various modes of action. This article uses the collective experience of three laboratories to provide information on pubertal assay conduct, ...
Peck George W GW Vector Control Department, Entomology Branch, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring,MD 20910, - - 2014
We tested an immunomarking system that used egg white as marker and enzyme-linked immunosorbent assay as a detection assay to characterize face fly (Musca autumnalis DeGeer) dispersal from cow pats in a pastured beef cattle operation. In microcage assays, adult flies acquired marker after contact with cow pats that were ...
Sisterson Mark S - - 2014
A method to improve an assay relating adult feeding to egg maturation by the glassy-winged sharpshooter (Hemiptera: Cicadellidae) was evaluated. The assay consisted on confining females to cowpea stems and quantifying feeding and egg maturation. Feeding was quantified by measuring excreta production. The number of eggs matured during the assay ...
Friedrich Nele N Metabolic Center, Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, - - 2014
Context: Measurement of IGFBP-3 can aid the diagnosis of growth hormone related diseases. Furthermore, epidemiological studies suggest that IGFBP-3 and the molar IGF-I/IGFBP-3 ratio are associated with clinical endpoints like cancer or cardiovascular disease. However, their clinical use is limited by the lack of validated reference intervals. Objectives: Establishment of ...
Gore M Odette MO Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, - - 2014
To determine the 99(th) percentile upper reference limit for the highly sensitive cardiac troponin T assay (hs-cTnT) in three large independent cohorts. The presently recommended 14 ng/L cutpoint for the diagnosis of myocardial infarction using the hs-cTnT assay was derived from small studies of presumably healthy individuals, with relatively little ...
Wang Xinfeng X 1Shandong Provincial Chest Hospital, Lab Medicine , Jinan , - - 2014
This study was performed aiming to evaluate the sensitivity of T-SPOT.TB assay in diagnosis of pediatric TB and investigate the association between age and results of T-SPOT.TB assay. Between March 2012 and September 2013, 102 patients, who aged ≤15 years old, were enrolled in this retrospective study and then were grouped ...
Wang Yuling Y Faculty of Chemistry and Center for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitaetsstr. 5, D-45141 Essen, Germany. - - 2014
Rapid parallel detection of two cytokines (IL-6 and IL-8) with femtogram sensitivity in a simple direct dot-blot assay is demonstrated. The microspectroscopic SERS acquisition scheme employs rationally designed, hydrophilically stabilized Au-Ag nanoshells as SERS labels, which are optimized for signal enhancement upon red laser excitation.
Fan Lie Ying LY Department of Clinical Laboratory, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, - - 2013
High-sensitivity cardiac troponin T (hs-cTnT) assay is used in the diagnosis and risk assessment of patients with symptoms of myocardial infarction. This study was undertaken to establish an age-specific 99th percentile cutoff value for hs-cTnT in Chinese population, and to evaluate its potential for early prediction of non-ST-segment elevation myocardial ...
Laffon Blanca B Toxicology Unit, Department of Psychobiology, University of A Coruña, Edificio de Servicios Centrales de Investigación, Campus Elviña s/n, 15071- A Coruña, Spain. Electronic address: - - 2013
The accident with the oil tanker Prestige in November 2002 resulted in a major spill of about 63,000 tons of heavy fuel oil. More than 300,000 people participated in the clean-up activities, which lasted for up to 10 months. Previous studies reported increases in genotoxicity endpoints in individuals exposed to ...
Nelson Scott M - - 2013
To develop an optimal model and age-specific centiles for the decline in antimüllerian hormone (AMH) as measured by the new Beckman Coulter AMH Generation II (Gen II) assay and compare this to the previous nomogram derived for the Diagnostics Systems Laboratory (DSL) assay. Multicenter retrospective population study, with validation of ...
Veldhuis Johannes D - - 2013
Context.SHBG concentrations correlate inconsistently with metabolic parameters.Hypothesis.SHBG assay platforms contribute to nonuniformities in the literature.Design.Noninterventional quantification of SHBG by 2 immuno- and 2 mass-spectrometric assays, and abdominal visceral fat (AVF) by CT scan.Setting.Center for Translational Science Activities (CTSA).Participants.Healthy men (N=120) ages 18-80 yr with BMI 20-43 kg/m(2).Outcomes.Correlation of log SHBG ...
Martin M D MD Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, - - 2013
In this study, female preferences and behavioural isolation were estimated in a pair of allopatric sister species, Etheostoma duryi and Etheostoma flavum. Dichotomous mate preference trials were conducted to determine whether females prefer to associate with conspecific over heterospecific males and free-spawning assays were conducted to determine whether those preferences ...
Ajitha B - - 2013
In this paper we report the green synthesis of silver nanoparticles (Ag NPs) using Tephrosia purpurea leaf extract. The biomolecules present in the leaf extract are responsible for the formation of Ag NPs and they found to play dual role of both reducing as well as capping agents. The high ...
Chung Tae-Wook - - 2013
Advanced glycation end-products (AGEs) play key roles in the development of diabetic vascular complications by activating the proliferation and migration of vascular smooth muscle cells. Here, we identified an increase of the migratory properties of human aortic smooth muscle cells (HASMC) through AGE-induced expression of lipocalin-2 (LCN2). Because the AGE-elicited ...
Knudsen Cindy Soendersoe - - 2013
Abstract Background: Recently, measurement of amino terminal propeptide of type III procollagen (PIIINP) was introduced as a part of the hepatic cirrhotic marker enhanced liver fibrosis™ test on the automated ADVIA Centaur® immunoassay platform (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). In this article, we show that the Centaur PIIINP ...
Morovat Alireza - - 2013
Abstract Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen-I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference intervals for PINP in both adults and children. Methods: Assay imprecision, recovery and interference were studied. Serum and plasma values were compared, and ...
Ayukekbong J A - - 2013
SUMMARY We studied the seasonal fluctuation of norovirus and other enteric viruses in Cameroon. Two hundred participants aged between 1 and 69 years were prospectively followed up. Each participant provided monthly faecal samples over a 12-month period. A total of 2484 samples were tested using multiplex real-time PCR assay for ...
Stufano F - - 2013
von Willebrand disease (VWD) is caused by a quantitative and/or qualitative deficiency of the von Willebrand factor (VWF). The laboratory diagnosis of VWD is dependent on the measurement of VWF antigen (VWF:Ag) and ristocetin cofactor activity (VWF:RCo). The aim of this study was to undertake a two-centre evaluation of two ...
Koerbin Gus - - 2013
Using objective laboratory and clinical criteria to more accurately determine the 99th percentile values for cardiac troponin I and T. We measured cardiac troponin T and cardiac troponin I with high-sensitiivty assays in a large cohort of apparently healthy community subjects and calculated 99th percentiles for different sexes and ages. ...
Hariri Fatemeh Zahra - - 2013
The objective of the current study was to translate and test psychometric properties of the Premenstrual Symptoms Screening Tool (PSST) in Iran. Using a standard "forward-backward" procedure, the English version of PSST was translated into Persian. A random sample of university students aged 18 years and over completed the questionnaire in ...
Nordfors Cecilia - - 2013
The rise in human papillomavirus (HPV) infection has been suggested to be responsible for the increased incidence of oropharyngeal cancer in the Western world. This has boosted interest in oral HPV prevalence and whether HPV vaccines can prevent oral HPV infection. In a previous study we showed oral HPV prevalence ...
Wethington Holly - - 2013
Among school-aged youth, we sought to identify characteristics associated with (1) exceeding screen time recommendations (ie, television/videos/video games more than 2 hours/weekday), and (2) exceeding screen time recommendations, the presence of a television in the bedroom, and obesity. Using 2007 National Survey of Children's Health data, we used multivariable logistic ...
Jia Kun - - 2013
The effects of carbofuran toxicity on a genetically modified bacterial strain E. coli DPD2794 were enhanced using a new bioluminescent protocol which consisted of three consecutive steps: incubation, washing and luminescence reading. Specifically, in the first step, several concentrations of carbofuran aqueous solutions were incubated with different bacterial suspensions at ...
Siangcham Tanapan - - 2013
Neurotransmitters and neurohormones are agents that control gonad maturation in decapod crustaceans. Of these, serotonin (5-HT) and dopamine (DA) are neurotransmitters with known antagonist roles in female reproduction, whilst gonadotropin-releasing hormones (GnRHs) and corazonin (Crz) are neurohormones that exercise both positive and negative controls in some invertebrates. However, the effects ...
Petersmann Astrid - - 2013
Abstract Background: The IFCC Task Force on Clinical Applications of Cardiac Biomarkers suggests comparing several contemporary sensitive troponin assays in the same, sufficiently large reference population. Methods: Three contemporary sensitive assays were used to measure troponin I concentration in samples from a uniquely large healthy population (2404 individuals) and in ...
Gannagé-Yared Marie-Hélène - - 2013
OBJECTIVE: Third generation parathyroid hormone (PTH) assays are new generation assays that do not recognize the PTH7-84 fragment whereas second generation assays detect both PTH1-84 and PTH7-84 fragments. Despite the excellent correlation between both assays in chronic renal failure (CRF) subjects, the mean PTH levels are typically 50% lower with ...
Regan Jamie - - 2013
Objective: To assess the properties of a novel body wash containing the mild surfactant glycinate. Design: Biochemical and clinical assays. Setting: Research laboratories and clinical sites in the United States and Canada. Participants: Women 18 to 65 years of age (cleansing efficacy); male and female subjects 26 to 63 years ...
Ginjupalli Gautam K - - 2013
Pyriproxyfen is an insecticidal juvenile hormone analog that perturbs insect and tick development. Pyriproxyfen also alters parthenogenic reproduction in non-target cladoceran species as it induces male production that can lead to a decrease in fecundity, a reduction in population density, and subsequent ecological effects. In this study, we investigate the ...
Anderson Joseph C - - 2013
BACKGROUND: & Aims: The adenoma detection rate (ADR) is an important quality indicator originally developed for screening colonoscopies. However, it is unclear whether the ADR should be calculated using data from screening and surveillance examinations. The recommended benchmark ADR for screening examinations is 20% (15% for women and 25% for ...
Delves Michael J - - 2013
It is the mature gametocytes of Plasmodium that are solely responsible for parasite transmission from the mammalian host to the mosquito. They are therefore a logical target for transmission blocking antimalarial interventions which aim to break the cycle of re-infection and reduce the prevalence of malaria cases. Gametocytes however are ...
McKie Paul M - - 2013
BACKGROUND: High-sensitivity cardiac troponin (hs-cTn) assays are now available that can detect measurable troponin in significantly more individuals in the general population than conventional assays. The clinical use of these hs-cTn assays depends on the development of proper reference values. Therefore, our objective was to define hs-cTnI reference values and ...
Aw Tar-Choon - - 2013
BACKGROUND: As new high-sensitivity (hs) cardiac troponin (cTn) assays are introduced decision limits, based on the 99th percentile upper reference limit (99 percentile URL), for each method must be determined and from a sufficiently large cohort to mitigate against distortionary effects of high-end outliers. There is a paucity of studies ...
Verfaillie C J - - 2013
INTRODUCTION: The diagnosis of von Willebrand disease (VWD) largely depends on the results of von Willebrand factor (VWF) antigen and activity. Recently, a new automated VWF:RCo assay on Acustar was developed. This assay panel for VWD also contains a new antigen (VWF:Ag) test. In this study, both chemiluminescence tests (HemosIL ...
McGurk Paula - - 2013
This study examined an electronic nutritional self-screening procedure for feasibility and for reliability, rapidity, and ease of use by hospital outpatients. One hundred sixty consecutive patients (ages 18-87 y) attending a gastroenterology clinic measured their weight and height using a modified digital weight and height machine, which transmitted results to ...
Gomes Luis Alexandre Piteira - - 2013
Rodent scent-marking is often used for territorial defence and self-advertisement, and both functions often entail the continuous scent-marking of a large area with high costs. In species with highly-fragmented populations and low density, in which the likelihood of social encounters is low, the costs of continuous scent-marking might exceed the ...
Hashiyama Aoi - - 2013
Autographa nigrisigna (Lepidoptera: Noctuidae) is the target species of the mating disruptant for vegetables, Confuser V. However, emergence of A. nigrisigna does not decrease in the Confuser V treated vegetable fields. As one of the reasons for this phenomenon, migration of mated females into the Confuser V treated area were ...
James Gemma L - - 2013
In the highly melioidosis-endemic Darwin region of Australia only 11/354 (3%) healthy residents were seropositive by indirect hemagglutination assay, despite extensive exposure to Burkholderia pseudomallei. None developed melioidosis but some described a prior self-limiting illness. This seropositivity rate is much lower than that seen in similarly highly melioidosis-endemic northeast Thailand, ...
Yue Hua - - 2013
Aim:PRD1-BF-1-RIZ1 homologous domain containing protein-16 (PRDM16) is a cell-autonomous transcriptional component that stimulates the development of brown fat cells. The aim of this study was to investigate the contribution of genetic variants of PRDM16 to obesity-related phenotype variations in Chinese.Methods:A total of 3204 subjects (consisting of 400 male-offspring nuclear families, ...
Kolkmeier Mark A - - 2013
Behavioral bioassays with the model freshwater cladoceran Daphnia magna have the potential to serve as nontraditional but sensitive endpoints of sublethal stress. However, few studies have examined the comparative sensitivity of neonate phototaxis perturbations to standardized endpoints commonly employed in chronic toxicity testing protocols. Even less understood are the consequences ...
Eggers Kai M - - 2013
BACKGROUND: Cardiac troponin levels are often detectable in community-dwellers when sensitive assays are applied. However, information on the course of troponin levels over time is limited. OBJECTIVES: We assessed changes in troponin levels, underlying conditions and the prognostic implications thereof in elderly subjects from the community. METHODS: Cardiac troponin I ...
Xian Bo - - 2013
Caenorhabditis elegans is a leading model organism for studying the basic mechanisms of aging. Progress has been limited, however, by the lack of an automated system for quantitative analysis of longevity and mean life span. To address this barrier, we developed "WormFarm", an integrated microfluidic device for culturing nematodes. Cohorts ...
Burks Charles S - - 2013
The sampling range of pheromone traps for the navel orangeworm Amyelois transitella (Walker) (Lepidoptera: Pyralidae) and its association with abundance was investigated by examining mutual interference within cross-shaped arrays of nine wing traps baited with virgin females and placed at 400-m intervals in three 256-ha blocks of almonds (Prunus dulcis ...
Mossner Maximilian - - 2013
Investigation of X-chromosome inactivation patterns (XCIP) by determination of differential CpG-methylation has been widely applied for investigation of female cell clonality. Using this approach the clonal origin of various tumours has been corroborated. Controversially, strong age-related increase of peripheral blood (PB) cell clonality in haematologically healthy female subjects was reported. ...
Ran Xiang - - 2013
We demonstrate a novel, rapid and label-free assay for the detection of Ag(+) and biothiols with high sensitivity and selectivity by utilization of Ag nanoparticle-decorated graphene quantum dots.
Choi Jae Young - - 2013
To investigate the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), and Ureaplasma urealyticum (UU) in first-voided urine samples and to determine the factors associated with positivity for sexually transmissible microorganisms in healthy, middle-aged Korean men. Five hundred fifty-one men who came to the hospital for a ...
1 2 3 4 5 6 7 8 9 10 >