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Smutny Michael M Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, - - 2015
Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess ...
Tsai Chih-Ming CM Genomics Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang District, Taipei 115, - - 2015
Plasma cells are terminally differentiated B cells that develop via the stimulation of mature B cells with various agents such as antigens and mitogens. Recently, we found that plasma cell differentiation can be modulated by galectin-1 and galectin-8; these galectins appear to play additive and redundant roles in promoting the ...
Liesche Johannes J Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark, - - 2015
Movement of nutrients and signaling compounds from cell to cell is an essential process for plant growth and development. To understand processes such as carbon allocation, cell communication, and reaction to pathogen attack it is important to know a specific molecule's capacity to pass a specific cell wall interface. Transport ...
White Rosemary G RG Black Mountain Laboratories, CSIRO Plant Industry, GPO Box 1600, Clunies Ross Street, Black Mountain, ACT, 2601, Australia, - - 2015
To investigate plasmodesmata (PD) function, a useful technique is to monitor the effect on cell-to-cell transport of applying an inhibitor of a physiological process, protein, or other cell component of interest. Changes in PD transport can then be monitored in one of several ways, most commonly by measuring the cell-to-cell ...
Kurome Mayuko M Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377, Munich, - - 2015
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, ...
Sumer Huseyin H Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, VIC, 3168, Australia, - - 2015
Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes ...
Diaz David D CNB-CSIC R&D Associated Unit, Department of Medicine, University of Alcalá, Carretera Madrid-Barcelona, Km 33.600, 28871, Alcalá de Henares, Madrid, Spain, - - 2015
Most authors currently quantify the frequency of apoptotic cells in a given phenotypically defined population after calculating the apoptotic index (AI), i.e., the percentage of apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this ...
Wieckowski Mariusz R MR Laboratory of Bioenergetics and Biomembranes, Department of Biochemistry, Nencki Institute of Experimental Biology, Pasteur 3 St., 02-093, Warsaw, Poland, - - 2015
Mitochondria are intracellular organelles where most fundamental processes of energy transformation within the cell are located. They also play important roles in programmed cell death (apoptosis), free radical formation, and signal transduction. In addition, mitochondria host genes encoding several important proteins. Studying isolated mitochondria is therefore crucial for better understanding ...
Ahmed Omar O Charité Campus Mitte, Institute of Pathology, Charitéplatz 1, Berlin, 10117, - - 2015
RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the ...
Mullen C A CA Centre for Biomechanics Research (BMEC), Department of Biomedical Engineering, NUI Galway, Galway, Republic of Ireland National Centre for Biomedical Engineering Science (NCBES), NUI Galway, Galway, Republic of - - 2014
Extracellular mechanical cues have been shown to have a profound effect on osteogenic cell behaviour. However, it is not known precisely how these cues alter intracellular mechanics to initiate changes in cell behaviour. In this study, a combination of in vitro culture of MC3T3-E1 cells and finite-element modelling was used ...
Wang Chenlu C Biophysics Graduate Program, University of Maryland, College Park, MD 20742, USA Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, 37 Convent Drive, Bethesda, MD 20892, USA - - 2014
Collective cell migration often involves notable cell-cell and cell-substrate adhesions and highly coordinated motion of touching cells. We focus on the interplay between cell-substrate adhesion and cell-cell adhesion. We show that the loss of cell-surface contact does not significantly alter the dynamic pattern of protrusions and retractions of fast migrating ...
Norton Kerri-Ann KA Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA - - 2014
It is very important to understand the onset and growth pattern of breast primary tumours as well as their metastatic dissemination. In most cases, it is the metastatic disease that ultimately kills the patient. There is increasing evidence that cancer stem cells are closely linked to the progression of the ...
Gasperini Luca L http://orcid.org/0000-0003-2133-4379 3B's, Department of Polymer Engineering, University of Minho, 4806-909 Caldas das Taipas, Portugal ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, - - 2014
The encapsulation of living mammalian cells within a semi-permeable hydrogel matrix is an attractive procedure for many biomedical and biotechnological applications, such as xenotransplantation, maintenance of stem cell phenotype and bioprinting of three-dimensional scaffolds for tissue engineering and regenerative medicine. In this review, we focus on naturally derived polymers that ...
Ding Yi Y State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, - - 2014
A polymerizable disulfide paclitaxel (PTX) prodrug was synthesized by the consequential esterification reactions of 3,3'-dithiodipropionic acid (DTPA), a disulfide compound containing two active carboxyl groups, with 2-hydroxyethyl methacrylate (HEMA) and PTX. The structure of the prodrug was confirmed by (1)H NMR characterization. Then, the polymerizable prodrug was copolymerized with poly(ethylene ...
Beke S S Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. Electronic address: - - 2014
The development of bioactive materials is in the premise of tissue engineering. For several years, surface functionalization of scaffolds has been one of the most promising approaches to stimulate cellular activity and finally improve implant success. Herein, we describe the development of a bioactive composite scaffold composed of a biodegradable ...
Wang Xiao Li XL Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, - - 2014
Human umbilical cord mesenchymal stromal cells (hUC-MSCs) hold great potential as a therapeutic candidate to treat diabetes, owing to their unlimited source and ready availability. In this study, we differentiated hUC-MSCs with in vitro-synthesized pancreatic-duodenal homebox 1 (PDX1) messenger (m)RNA into islet-like cell clusters. hUC-MSCs were confirmed by both biomarker ...
Liu Ronghua R Department of Immunology, Shanghai Medical College, Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University, Shanghai 200032, P.R. China ; Biotherapy Research Center of Fudan University, Shanghai 200032, P.R. - - 2014
Paclitaxel (PTX) is the front-line chemotherapeutic agent against human non-small cell lung cancer (NSCLC). However, its therapeutic efficacy is restricted by the increasing frequency of chemotherapeutic resistance in NSCLC. Accumulating evidence has shown the potential role of microRNAs (miRNAs) in the chemotherapeutic sensitivity of cancer cells. Previously it was reported ...
Tomizawa Minoru M Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, - - 2014
Resistance is one limitation of sorafenib in the treatment of hepatocellular carcinoma (HCC). Insulin-like growth factor-1 receptor (IGF-1R) is involved in cancer cell proliferation. To assess the potential synergistic antitumor effects of picropodophyllin (PPP), an IGF-1R inhibitor, HLF and PLC/PRL/5, HCC cells were treated with PPP alone or PPP in ...
Wang Yong Y Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. - - 2014
Long non-coding RNAs (lncRNAs) are novel regulators in cancer biology. BRAF-activated lncRNA (BANCR) is overexpressed in melanoma and has a potential functional role in melanoma cell migration. However, little is known about the role of BANCR in the development of papillary thyroid carcinoma (PTC). In the present study, BANCR expression ...
Wang Ding D Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China ; Key Laboratory of Reproduction and Genetics, Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China ; Experimental Department, Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. - - 2014
The present study aimed to improve the characterization of amniotic fluid cells (AFCs) in order to optimize their use in chromosomal prenatal diagnosis and as seed or stem cells for tissue engineering. The AFCs used in the current study were obtained from three females in their second trimester of pregnancy. ...
DU Xue X Department of Obstetrics and Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. - - 2014
The aim of the present study was to investigate the effect of heat shock protein 90 (Hsp90)-specific inhibitor geldanamycin (GA) on the proliferation and apoptosis induced by vascular endothelial growth factor-C (VEGF-C) in cervical cancer cells. HeLa cells (1×10(6)/ml) in the logarithmic growth phase were incubated without serum for 24 ...
Rouse Michael M Laboratory of Clinical InvestigationLaboratory of Cardiovascular ScienceNational Institute on Aging, Intramural Research Program, National Institutes of Health, 251 Bayview Blvd, Baltimore, Maryland 21224, - - 2014
Resveratrol (RES) and curcumin (CUR) are polyphenols that are found in fruits and turmeric, and possess medicinal properties that are beneficial in various diseases, such as heart disease, cancer, and type 2 diabetes mellitus (T2DM). Results from recent studies have indicated that their therapeutic properties can be attributed to their ...
Zuo Yanzhen Y Department of Pharmacology, Chengde Medical University, Chengde, Hebei 067000, P.R. - - 2014
Transforming growth factor-β1 (TGF-β1) is involved in the regulation of trophoblast cell proliferation and invasion. However, the mechanism underlying this process remains unknown, which is predominantly due to the difficulty in obtaining and maintaining primary trophoblast cells in culture over a long period of time. The HTR-8/SVneo cell line is ...
Wang Bing B Department of General Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. - - 2014
Decitabine (DAC), an inhibitor of DNA methyltransferase, demonstrates antitumor activities in various types of cancer. However, its therapeutic potential for cholangiocarcinoma (CCA), one of the most aggressive gastrointestinal malignancies, remains to be explored. The present study investigated the antiproliferative effects of DAC on CCA cells in vitro and in vivo. ...
Dong Hanzhang H Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. - - 2014
The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression ...
Pelton John C JC Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, - - 2014
Blood vessel polarization in the apical-basal axis is important for directed secretion of proteins and lumen formation; yet, when and how polarization occurs in the context of angiogenic sprouting is not well understood. Here, we describe a novel topology for endothelial cells at the tip of angiogenic sprouts in several ...
Ichwan Solachuddin J A SJ Department of Basic Medical Science, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan 25710, Pahang, Malaysia Human Molecular Cellular Biology Research Unit, Integrated Centre of Research and Animal Care and Use (HMCB-ICRACU), International Islamic University, Kuantan, Pahang, - - 2014
Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil which shows promising in vitro and in vivo anti-neoplastic activities in different tumor cell lines. However, to date there are only a few reports regarding the apoptotic effects of TQ on cervical cancer cells. Here, we ...
Shajahan-Haq Ayesha N - - 2014
About 70% of all breast cancers are estrogen receptor alpha positive (ER+) and are treated with antiestrogens. However, 50% of ER + tumors develop resistance to these drugs (endocrine resistance). In endocrine resistant cells, an adaptive pathway called the unfolded protein response (UPR) is elevated that allows cells to tolerate ...
Chanasakulniyom Mayuree M The Division of Biomedical Engineering, School of Engineering, The University of Glasgow, G12 8LT Glasgow, UK. - - 2014
Cell proliferation and migration are fundamental processes in determining cell and tissue behaviour. In this study we show the design and fabrication of a new single cell microfluidic structure, called a "vertically integrated array" or "VIA" trap to explore quantitative functional assays including single cell attachment, proliferation and migration studies. ...
Zhu Xiao-San XS Department of Gastroenterology, Chenggong Hospital Affiliated to Xiamen University, Xiamen, Fujian, 361003, China, - - 2014
Enoyl coenzyme A hydratase short chain 1 (ECHS1) is an important part of the mitochondrial fatty acid β-oxidation pathway. Altered ECHS1 expression has been implicated in cancer cell proliferation. This study assessed ECHS1 expression in human gastric cancer cell lines and investigated the effects of ECHS1 knockdown on gastric cancer ...
Kim Jong Ah JA Centre for BioNano Interactions, School of Chemistry and Chemical Biology and Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. kenneth.a.dawson@cbni.ucd.ie - - 2014
Nanomaterials challenge paradigms of in vitro testing because unlike molecular species, biomolecules in the dispersion medium modulate their interactions with cells. Exposing cells to nanoparticles known to cause cell death, we observed cytotoxicity suppression by increasing the amount of serum in the dispersion medium towards in vivo-relevant conditions.
Nakayama Tomofumi T Graduate School of Science and Technology, Kumamoto University, Kumamoto, - - 2014
The mechanism of neutrophil extracellular trap cell death (NETosis), a regulated cell death pathway relevant to infection, autoimmunity and sepsis, is not completely known. The reason for this, at least in part, is the lack of an in vitro system that recapitulates the NETosis pathway using established human cell lines. ...
Kimura S S 1] Laboratory of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan [2] Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Kanagawa, - - 2014
The microfold (M) cell residing in the follicle-associated epithelium is a specialized epithelial cell that initiates mucosal immune responses by sampling luminal antigens. The differentiation process of M cells remains unclear due to limitations of analytical methods. Here we found that M cells were classified into two functionally different subtypes ...
Choi Jong Min JM College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of - - 2014
Although various in vitro assays have been developed to evaluate the cytochrome P450 (CYP)-inducing potential of drug candidates, there is a continuing need for the development of a reliable model in drug discovery. The objective of the present study was to compare CYP induction by chemicals in HepG2 cells with ...
Wu Minjuan M Department of Histology and Embryology, Second Military Medical University, Shanghai, 200433, China; Burns Institute of People's Liberation Army, Changhai Hospital, The Second Military Medical University, Shanghai, 200433, - - 2014
The mesenchymal to epithelial transition (MET) occurs in organ development and anti-tumorigenesis. We have investigated the effects of calcium (Ca(2+) ) and epidermal growth factor (EGF) on human mesenchymal stem cell (hMSCs) differentiation into epithelial-like cells. hMSCs lost their biological characteristics after EGF transfection, and MET was achieved by adding ...
Yu Hui-Ping HP Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. - - 2014
Glioblastoma is the most common type of malignant brain tumor with a poor prognosis. The Notch signaling pathway is often aberrantly activated in glioma cells. In order to determine the expression of Notch 2 and to evaluate its possible prognostic value in malignant glioblastoma, specimens from 32 patients and 20 controls were ...
Kang Mi Ae MA Research Center for Radiotherapy, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of - - 2014
The tumor suppressor p53 plays a key role in regulation of the cell cycle, apoptosis and senescence in response to various stresses. We screened a library of 7920 chemical compounds for the p53 activator and identified N-[2-(dimethylamino)ethyl]-2,3-dimethyl-4-oxo-4H-pyrido[1,2-a]thieno[2,3-d]pyrimidine-9-carboxamide (PTP), which significantly increased p53-mediated reporter activity in colorectal cancer cells. PTP was ...
Kim Nak Hyun NH Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of - - 2014
Xanthomonas campestris pv. vesicatoria (Xcv) type III effector AvrBsT triggers programmed cell death (PCD) and activates the hypersensitive response (HR) in plants. Here, we isolated and identified the plasma-membrane localized pathogenesis-related (PR) protein 4c gene (CaPR4c) from pepper (Capsicum annuum) leaves undergoing AvrBsT-triggered HR cell death. CaPR4c encodes a protein ...
Rivers Orion S OS Department of Microbiology, University of Hawai'i at Mānoa, - - 2014
Formation and maintenance of a periodic pattern of nitrogen-fixing cells called heterocysts by the filamentous cyanobacterium Anabaena sp. strain PCC 7120 is dependent on regulators encoded by patS and hetN. In this study, genetic mosaic filaments that consisted of cells engineered to produce one of the developmental regulators flanked by ...
van der Geest Kornelis S M KS Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Department of Pathology and Medical Biology and Department of Laboratory Medicine, Section Medical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Department of Pathology and Medical Biology and Department of Laboratory Medicine, Section Medical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. - - 2014
The aim of this study was to investigate the turnover of Treg cells in the SF of RA patients. Treg cells were enumerated in peripheral blood and SF of RA patients and analysed by flow cytometry for expression of the proliferation marker Ki-67 and binding of the apoptosis marker annexin ...
Motomura Eriko E Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Kagoshima, - - 2014
Wnt proteins are thought to bind to their receptors on the cell surfaces of neighboring cells. Wnt8 likely substitutes for the dorsal determinants in Xenopus embryos to dorsalize early embryos via the Wnt/β-catenin pathway. Here, we show that Wnt8 can dorsalize Xenopus embryos working cell autonomously. Wnt8 mRNA was injected ...
Tao Ling L Department of Food Science, The Pennsylvania State University, University Park, PA, 16802, - - 2014
We have previously reported that the green tea catechin, (-)-epigallocatechin-3-gallate (EGCG), can induce oxidative stress in oral cancer cells but exerts antioxidant effects in normal cells. Here, we report that these differential pro-oxidative effects are associated with sirtuin 3 (SIRT3), an important mitochondrial redox modulator. EGCG rapidly induced mitochondria-localized reactive ...
Busch Maike M Department of Neuroanatomy, Medical Faculty, Institute of Anatomy, University of Duisburg-Essen, Hufelandstr. 55, 45122, Essen, - - 2014
Retinoblastoma (RB) is the most common malignant intraocular childhood tumor. Forty years after their first description, in the present study, we re-characterized seven established retinoblastoma cell lines with regard to their RB1 mutation status, morphology, growth pattern, endogenous apoptosis levels, colony formation efficiency in soft agar and invasiveness and dissemination ...
Bannunah Azzah M - - 2014
This study investigated the effect of nanoparticle size and surface charge on their interaction with Caco-2 monolayers as a model of the intestinal epithelium, including cell internalization pathways and the level of transepithelial transport. Initially, toxicity assays showed that cell viability and cell membrane integrity were dependent on the surface ...
Orzechowska Emilia Joanna - - 2014
Low cellular level of BID is critical for viability of numerous cancer cells. Sensitization of cells to anticancer agents by BID overexpression from adenovirus or pcDNA vectors is a proposed strategy for cancer therapy; however it does not provide any stringent control of cellular level of BID. The aim of ...
Feng Liang L Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148 Baojian Road, Nangang District, Harbin, 150086, - - 2014
Ser/Thr protein phosphatase 5 (PPP5C) has been reported to participate in tumor progression. However, its functional role in hepatocellular carcinoma (HCC) remains unknown yet. In this study, we firstly evaluated the expression levels of PPP5C in six HCC cell lines by real-time PCR and found that PPP5C was widely expressed ...
Tsang Matthew M Departments of Dentistry and Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, Dental Sciences Bldg., London, ON, N6A 5C1, - - 2014
Connective tissue growth factor (CTGF/CCN2), a member of the CCN family of matricellular proteins is upregulated in both fibrosis as well as tissue repair. Recently, we showed that, in mice, CCN2 expression by fibroblasts was required for dermal fibrogenesis, but not for cutaneous tissue repair. Lineage tracing analysis linked the ...
Bai Haihua H Department of Chemistry and Institute for Nano-Chemistry, Jinan University, Guangzhou, - - 2014
Apigenin is a flavonoid, which has been proved to possess effective anti-cancer bioactivities against variety of cell lines. However, little is known about its effect on the cell-surface and the interaction between cell-surface and the reacting drug. In this study, human breast cancer line (MCF-7) was selected to be as ...
Stachowiak Radosław R Department of Applied Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland, - - 2014
Internalins comprise a class of Listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. In this paper, a possible role of Lmo0171-a new member of the internalin family was investigated. Disruption of the lmo0171 gene resulted in important cell ...
Thimmegowda N R NR World Class Institute (WCI), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 363-883, Republic of Korea; Department of chemistry, Govt.S K S J T Institute, 560001, Bangalore, Karnataka, - - 2014
In the present study we have synthesized novel water soluble derivatives of natural compound aloe emodin 4(a-j) by coupling with various amino acid esters and substituted aromatic amines, in an attempt to improve the anticancer activity and to explore the structure-activity relationships. The structures of the compounds were determined by ...
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