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Mohammadi Hamid H Matrix Dynamics Group, University of Toronto, Toronto, Ontario, Canada - - 2015
The mechanical properties of extracellular matrix proteins strongly influence cell-induced tension in the matrix, which in turn influences cell function. Despite progress on the impact of elastic behaviour of matrix proteins on cell-matrix interactions, little is known about the influence of inelastic behaviour, especially at the large and slow deformations ...
Smutny Michael M Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, - - 2015
Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess ...
Tsai Chih-Ming CM Genomics Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang District, Taipei 115, - - 2015
Plasma cells are terminally differentiated B cells that develop via the stimulation of mature B cells with various agents such as antigens and mitogens. Recently, we found that plasma cell differentiation can be modulated by galectin-1 and galectin-8; these galectins appear to play additive and redundant roles in promoting the ...
Liesche Johannes J Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark, - - 2015
Movement of nutrients and signaling compounds from cell to cell is an essential process for plant growth and development. To understand processes such as carbon allocation, cell communication, and reaction to pathogen attack it is important to know a specific molecule's capacity to pass a specific cell wall interface. Transport ...
White Rosemary G RG Black Mountain Laboratories, CSIRO Plant Industry, GPO Box 1600, Clunies Ross Street, Black Mountain, ACT, 2601, Australia, - - 2015
To investigate plasmodesmata (PD) function, a useful technique is to monitor the effect on cell-to-cell transport of applying an inhibitor of a physiological process, protein, or other cell component of interest. Changes in PD transport can then be monitored in one of several ways, most commonly by measuring the cell-to-cell ...
Kurome Mayuko M Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377, Munich, - - 2015
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, ...
Sumer Huseyin H Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, VIC, 3168, Australia, - - 2015
Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes ...
Diaz David D CNB-CSIC R&D Associated Unit, Department of Medicine, University of Alcalá, Carretera Madrid-Barcelona, Km 33.600, 28871, Alcalá de Henares, Madrid, Spain, - - 2015
Most authors currently quantify the frequency of apoptotic cells in a given phenotypically defined population after calculating the apoptotic index (AI), i.e., the percentage of apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this ...
Wieckowski Mariusz R MR Laboratory of Bioenergetics and Biomembranes, Department of Biochemistry, Nencki Institute of Experimental Biology, Pasteur 3 St., 02-093, Warsaw, Poland, - - 2015
Mitochondria are intracellular organelles where most fundamental processes of energy transformation within the cell are located. They also play important roles in programmed cell death (apoptosis), free radical formation, and signal transduction. In addition, mitochondria host genes encoding several important proteins. Studying isolated mitochondria is therefore crucial for better understanding ...
Ahmed Omar O Charité Campus Mitte, Institute of Pathology, Charitéplatz 1, Berlin, 10117, - - 2015
RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the ...
Rocheteau Pierre P Human histopathology and animal models, Institut Pasteur, 28 rue du Dr. Roux, 75724, Paris Cedex 15, - - 2015
The skeletal muscle of vertebrates has a huge regenerative capacity. When destroyed after different types of injury, this organ can regenerate very quickly (less than 20 days following myotoxin injection in the mouse) ad integrum and repeatedly. The cell responsible for this regeneration is the so-called satellite cell, the muscle ...
Bose Mayil Vahanan MV Department of Molecular Oncology, Cancer Institute (WIA), 38, Sardar Patel Road, Chennai, 600036, - - 2015
The aim of radiotherapy is to kill tumor cells in a primary tumor, in draining lymph nodes, and/or in small metastatic lesions. The response of tumor cells to radiation depends on the dose, an individual's radiosensitivity, the duration of radiation exposure (i.e., the timing), the fraction size, and the presence ...
Massie Isobel I Department of Ocular Biology and Therapeutics, Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, - - 2015
The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors. The use of advanced imaging techniques is described to visualize the niche in three dimensions in native human corneal tissue. ...
Rydahl Maja G MG Department of Plant and Environmental Sciences, Faculty ofScience, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Copenhagen, - - 2015
The growth of a plant cell encompasses a complex set of subcellular components interacting in a highly coordinated fashion. Ultimately, these activities create specific cell wall structural domains that regulate the prime force of expansion, internally generated turgor pressure. The precise organization of the polymeric networks of the cell wall ...
Kacprzyk Joanna J School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, - - 2015
Programmed cell death can be defined as an organized cellular destruction and can be activated throughout plant development, as a defense response against invading pathogens or during environmental stress. The root hair assay presented herein enables in vivo quantitative measurements of programmed cell death based on the morphological changes of ...
Alasia Silvia S Department of Veterinary Sciences, University of Turin, Via Leonardo da Vinci 44, Turin, Grugliasco, 10095, - - 2015
As apoptosisapoptosis occurs via a complex signaling cascade that is tightly regulated at multiple cell points, different methods exist to evaluate the activity of the proteins involved in the intracellular apoptotic pathwaysapoptotic pathways and the phenotypephenotype of apoptotic neuronsneurons . Detention of the activity of the enzyme caspase-3caspase-3 , the ...
Shin Hye Young HY Department of Neurosurgery, Seoul National University Hospital, Seoul, - - 2015
Angiographic findings suggest that central neurocytoma (CN) might originate from neuronal cells of the subventricular zone (SVZ) around the foramen of Monro rather than from the septum pellucidum. The majority of CN cells have neuroblast characteristics. Most importantly, CN-derived tumor spheres have a phenotype of transit-amplifying type C cells, implying ...
Li Qiu Q Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, - - 2014
The loss of or decreased functional pancreatic β-cell is a major cause of type 1 and type 2 diabetes. Previous studies have shown that adult β-cells can maintain their ability for a low level of turnover through replication and neogenesis. Thus, a strategy to prevent and treat diabetes would be ...
Carey Bryce W BW Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, New York 10065, - - 2014
The role of cellular metabolism in regulating cell proliferation and differentiation remains poorly understood. For example, most mammalian cells cannot proliferate without exogenous glutamine supplementation even though glutamine is a non-essential amino acid. Here we show that mouse embryonic stem (ES) cells grown under conditions that maintain naive pluripotency are ...
Chen Xin X Xin Chen, Jia Guo, Ru-Xing Xi, Yu-Wei Chang, Xiao-Zhi Zhang, Radiation Department, First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Provence, - - 2014
To investigate the expression of miR-210 and the role it plays in the cell cycle to regulate radioresistance in oesophageal squamous cell carcinoma (ESCC). MiR-210 expression was evaluated in 37 pairs of ESCC tissues and matched para-tumorous normal oesophageal tissues from surgical patients who had not received neoadjuvant therapy, and ...
Chang Chien-Hsin - - 2014
Hepatocellular carcinoma (HCC) has become one of most common malignancy and leading cause of cancer mortality worldwide. Previous study has shown that 4-acetylantroquinonol B (4AAQB) isolated from Antrodia cinnamomea (or niu-chang-chih) was observed to inhibit HepG2 cell proliferation via affecting cell cycle. However, the in vivo effects and anti-metastatic activity ...
Xu Xiao-Liang XL Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, P. R. China. xdyang@ynu.edu.cn - - 2014
A series of novel 2-substituted indoline imidazolium salt derivatives has been prepared and evaluated in vitro against a panel of human tumor cell lines. The results suggest that the existence of a substituted benzimidazole ring and substitution of the imidazolyl-3-position with a naphthylacyl or 2-naphthylmethyl group were vital for modulating ...
Zhuang Ming M Department of Oncology, Clinical Medical College of Yangzhou University, No. 98 Nantong Western Road, Yangzhou, 225001, People's Republic of - - 2014
We investigated the possible role of miR-143 in the development of cisplatin resistance in human gastric cancer cell line. miR-143 was detected by quantitative real-time PCR. Cisplatin resistance changes of cells was tested via MTT assay. Target genes of miR-143 were verified by dual-luciferase activity assay. Immunohistochemistry, immunofluorescence staining, Western ...
Zhang Qu Q Department of Radiotherapy, Hubei Cancer Hospital, Wuhan, - - 2014
The radioresistance of esophageal squamous cell carcinoma (ESCC) remains an obstacle for the effective radiotherapy of ESCC. This study aimed to investigate the radiosensitization of ESCC by signal transducer and activator of transcription 3 (STAT3) inhibitor stattic. ECA109, TE13, and KYSE150 cell lines were exposed to hypoxia and treated with ...
Qin Zhixiang - - 2014
BackgroundIn plants, the growth of an aerial organ to its characteristic size relies on the coordination of cell proliferation and expansion. These two different processes occur successively during organ development, with a period of overlap. However, the mechanism underlying the cooperative and coordinative regulation of cell proliferation and expansion during ...
Yu Jiashing - - 2014
Photodynamic therapy (PDT) involves the cellular uptake of a photosensitizer (PS) combined with oxygen molecules and light at a specific wavelength to be able to trigger cancer cell death via the apoptosis pathway, which is less harmful and has less inflammatory side effect than necrosis. However, the traditional PDT treatment ...
Zhang Wei W Departments of Comparative Medicine, Stanford University, Stanford, CA, - - 2014
Numerous hypotheses of temporal lobe epileptogenesis have been proposed, and several involve hippocampal mossy cells. Building on previous hypotheses we sought to test the possibility that after epileptogenic injuries surviving mossy cells develop into super-connected seizure-generating hub cells. If so, they might require more cellular machinery and consequently have larger ...
Gillani Tina B - - 2014
The multi-kinase inhibitor sorafenib (SRF) is approved for the treatment of renal and hepatic carcinomas and is also undergoing evaluation in therapeutic combinations with other anticancer agents. SRF is generally well tolerated but produces severe toxicities in a significant proportion of patients by mechanisms that are largely unknown. It has ...
Zhao Yi - - 2014
The presence of AT2 receptors in mitochondria and their role in NO generation and cell aging were recently demonstrated in various human and mouse non-tumour cells. We investigated the intracellular distribution of AT2 receptors including their presence in mitochondria and the role in the induction of apoptosis and cell death ...
Villegas Josefina M JM Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, 4000, San Miguel de Tucumán, - - 2014
The cell envelope-associated proteinase (CEP) of Lactobacillus delbrueckii subsp. lactis CRL 581 (PrtL) has an essential role in bacterial growth, contributes to the flavor and texture development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. The genome of L. delbrueckii subsp. lactis CRL 581 possesses only ...
Ye Ju Xiang JX Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, - - 2014
The presence of CD117 positive cells in esophageal leiomyoma may lead to a misdiagnosis of GIST. We reviewed 46 esophageal tumors which were smooth muscle tumors or GIST. Based on morphology, immunohistochemistry and mutation analysis, there were 44 (95.6%) leiomyomas, 1 (2.2%) leiomyosarcoma, and 1 (2.2%) GIST. Variable numbers of ...
Xu Jen Xin JX Brigham and Women's - - 2014
Interaction of polycystin-1 (PC1) and Gα12 is important for development of kidney cysts in autosomal dominant polycystic kidney disease (ADPKD). The integrity of cell polarity and cell-cell adhesions (mainly E-cadherin-mediated adherens junction) is altered in the renal epithelial cells of ADPKD. However, the key signaling pathway for this alteration is ...
Shao Xin X Department of Pathology, Changzhou Hospital of Traditional Chinese Medicine, Changzhou, 213003, Jiangsu Province, - - 2014
G protein-coupled receptors (GPCRs) are important signal transduction mediators and pharmacological therapeutic targets. G protein-coupled receptor 137 (GPR137) was initially reported as a novel orphan GPCR around 10 years ago. Some orphan GPCRs have been implicated in cancer cell proliferation and migration. The aim of this study is to investigate ...
McGrail Daniel J DJ School of Chemical & Biomolecular - - 2014
A growing body of evidence suggests that the developmental process of epithelial-to-mesenchymal transition (EMT) is co-opted by cancer cells to metastasize to distant sites. This transition is associated with morphologic elongation and loss of cell-cell adhesions, though little is known about how it alters cell biophysical properties critical for migration. ...
Gundara Justin S JS Cancer Genetics, Kolling Institute of Medical Research, St. Leonards, Sydney, New South Wales, Australia; Endocrine Surgical Unit, Royal North Shore Hospital, St. Leonards, Sydney, New South Wales, - - 2014
Micro-RNAs are dysregulated in medullary thyroid carcinoma (MTC) and preliminary studies have shown that miRNAs may enact a therapeutic effect through changes in autophagic flux. Our aim was to study the in vitro effect of miR-9-3p on MTC cell viability, autophagy and to investigate the mRNA autophagy gene profile of ...
Tseng Ruo-Chia RC *Department of Molecular Biology and Human Genetics, College of Life Science, Tzu Chi University, Hualien, Taiwan; †Department of Surgery, Chia-Yi Christian Hospital, Chiayi, Taiwan; ‡Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; §Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; ‖Department of Biomedical Engineering, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan; ¶Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; #Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; and **Department of Surgery, College of Medicine, National Cheng Kung University, Tainan, - - 2014
SLIT2, a secreted protein, has been found to inactivate Cdc42 GTPase to modulate neural cell migration. However, alteration of SLIT2-mediated Cdc42 in terms of migration regulation remains undefined in esophageal squamous cell carcinoma (ESCC). We report here in ESCC cell, animal, and clinical models that SLIT2 acts as a migration ...
Sabherwal Nitin N Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK. Electronic address: - - 2014
During the development of the nervous system, apicobasally polarized stem cells are characterized by a shorter cell cycle than nonpolar progenitors, leading to a lower differentiation potential of these cells. However, how polarization might be directly linked to the kinetics of the cell cycle is not understood. Here, we report that ...
Shin Yong-Hwan YH Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute, Seoul, - - 2014
Muscarinic receptors, particularly the type 3 subtype (M3R), have an important role in exocrine secretion. M3R normally function in HSG cells originated from human submandibular gland ducts, but not in A253 and SGT cells, derived from human submandibular carcinoma and salivary gland adenocarcinoma. However, the underlying mechanism of this suppression ...
Kang Ya'an Y Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, - - 2014
Many commercially available cell lines have been in culture for ages, acquiring phenotypes that differ from the original cancers from which these cell lines were derived. Therefore, research on new cell lines could improve the success rates of translational research in cancer. We have developed methods for the isolation and ...
Lee Won Sup WS Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of - - 2014
CKD‑712, a newly synthesized tetrahydroisoquinoline (THI) and an enantiomer (S form) of YS 49 (a derivative of higenamine) has been reported to suppress nuclear factor-κB (NF-κB) activity in normal cells. In the present study, we investigated the anticancer effects of THI at a low concentration where CKD-712 did not induce cell death in ...
Zhao Bao-Xia BX Laboratory Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. - - 2014
Non‑small cell lung cancer (NSCLC) cells harboring mutations in the epidermal growth factor receptor (EGFR) gene initially respond well to EGFR tyrosine kinase inhibitors (TKI), including gefitinib. However the tumor cells will invariably develop acquired resistance to the drug. The EGFR T790M mutation is generally considered to be the molecular ...
Xu Zongquan Z Deparment of Hepatic Oncology, Jiangxi Provincial Cancer Hospital, Nanchang 330029, P.R. - - 2014
Omi/HtrA2 promotes cell apoptosis in human cancer cells. Early studies showed that primary hepatocellular carcinoma requires Omi/HtrA2 expression for cell apoptosis. Additionally, the Omi/HtrA2 pro-apoptotic marker demonstrated a difference in some cell types. However, how the Omi/HtrA2 pro-apoptotic marker reacts during the process of hepatocellular carcinoma cell apoptosis remains to ...
Morelli Maria Beatrice - - 2014
There is evidence that calcium (Ca2+) increases the proliferation of human advanced prostate cancer (PCa) cells but the ion channels involved are not fully understood. Here, we investigated the correlation between alpha1D-adrenergic receptor (alpha1D-AR) and the transient receptor potential vanilloid type 1 (TRPV1) expression levels in human PCa tissues and ...
Zhao Chun-Xiong CX Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, - - 2015
Accumulating evidences indicated that hypoxia-induced factors and COX-2 play a important role in tumorigenesis in various human cancer. Yet, the relationship between HIFs and COX-2 in human renal cancer remains unclear. The present study was to examine the role of HIFs and COX-2 in the invasiveness and the resistance to ...
Luo Yuhao Y The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, - - 2014
Lycorine, a natural alkaloid, has been widely reported to possess potential efficacy against cancer. However, the anti-multiple myeloma mechanism of lycorine is not fully understood. In this study, the results demonstrated that lycorine is effective against multiple myeloma cell line ARH-77 via inducing programmed necrosis. The mechanisms of lycorine on ...
Gallala Hichem H Department of Hematology/Oncology, University of Bonn, Bonn, - - 2014
We have recently shown that staurosporine mediates the conversion of small cell lung carcinoma (SCLC) cells into a neuron-like process-bearing phenotype. Here, we have extended these studies to the staurosporine analogs K252a, lestaurtinib, PKC412, stauprimide, and UCN-01 and analyzed their influence on process extension, cell cycle distribution, and induction of ...
Zheng Hui-Xia HX Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, - - 2014
To explore the inhibitory effects of dobutamine on gastric adenocarcinoma cells. Dobutamine was used to treat gastric adenocarcinoma cells (SGC-7901) and cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of dobutamine combined with cisplatin on cell viability were also analyzed. Cell migration was studied using the ...
Park Seyeon S Department of Applied Chemistry, Dongduk Women's University, Seoul 136-714, - - 2014
The identification of small vesicles released by many cell types as tools of intercellular communication is proposed. Here, we identify SH-SY5Y neuroblastoma-derived exosomes comprised of major histocompatibility complex II (MHC II), Hsp90 and flotillin-1. Our data also suggest that, when applied extracellularly, exosomes released from neuronal cells stimulate dendrite-like outgrowth ...
Mullen C A CA Centre for Biomechanics Research (BMEC), Department of Biomedical Engineering, NUI Galway, Galway, Republic of Ireland National Centre for Biomedical Engineering Science (NCBES), NUI Galway, Galway, Republic of - - 2014
Extracellular mechanical cues have been shown to have a profound effect on osteogenic cell behaviour. However, it is not known precisely how these cues alter intracellular mechanics to initiate changes in cell behaviour. In this study, a combination of in vitro culture of MC3T3-E1 cells and finite-element modelling was used ...
Zhang Ren R College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, Zhengzhou, 450001, Henan, - - 2014
Potential target genes of microRNA (miR)-494 have been reported in many types of cancers. However, the role of miR-494 in esophageal squamous cell carcinoma (ESCC) remains unknown. This study focused on the expression and biological function of miR-494 in ESCC. Using bioinformatics analyses, we found that cleft lip and palate ...
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