Search Results
Results 351 - 400 of 1649
< 3 4 5 6 7 8 9 10 11 12 13 >
Kim Si Hyoung SH Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University, Chuncheon, Republic of - - 2014
The aim of the present study was to evaluate the effect of radicicol, an inhibitor of heat shock protein (hsp) 90, alone or in combination with hsp70 inhibition on survival of anaplastic thyroid carcinoma (ATC) cells. Antitumor activity of radicicol-alone or in combination with the hsp70 inhibitor VER155008 was investigated ...
Yamauchi Takahiro T Department of Hematology and Oncology, Faculty of Medical Sciences, University of Fukui, Matsuoka, Fukui, Japan - - 2014
Forodesine inhibits purine nucleoside phosphorylase, resulting in an accumulation of intracellular dGTP and consequently cell death. 9-β-D-Arabinofuranosylguanine (ara-G) is an active compound of nelarabine that is intracellularly phosphorylated to a triphosphate form, which inhibits DNA synthesis. Both agents show cytotoxicity toward T-cell malignancies. In the present study, we investigated the ...
Huang Weiyuan W Department of Physiology, Medical College, Jinan University, Guangzhou, - - 2014
We previously demonstrated that the growth of the poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z) was more dependent on the activities of volume-activated chloride channels than that of the normal nasopharyngeal epithelial cells (NP69-SV40T). However, the activities and roles of such volume-activated chloride channels in highly differentiated nasopharyngeal carcinoma cells (CNE-1) ...
Balsam Joshua J Division of Biology, Office of Science and Engineering, FDA, Silver Spring, MD 20993, United States; University of Maryland, College Park, MD 20742, United - - 2014
Detection of rare cells, such as circulating tumor cells, have many clinical applications. To measure rare cells with increased sensitivity and improved data managements, we developed an imaging flow cytometer with a streak imaging mode capability. The new streak mode imaging mode utilizes low speed video to capture moving fluorescently ...
Del Vecchio Catherine A CA Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of - - 2014
Malignant carcinomas that recur following therapy are typically de-differentiated and multidrug resistant (MDR). De-differentiated cancer cells acquire MDR by up-regulating reactive oxygen species (ROS)-scavenging enzymes and drug efflux pumps, but how these genes are up-regulated in response to de-differentiation is not known. Here, we examine this question by using global ...
Getchell Rodman G RG a Aquatic Animal Health Program, Department of Microbiology and Immunology, College of Veterinary Medicine , Cornell University , 930 Campus Road, Ithaca , New York 14853 , - - 2014
Abstract The development and characterization of a new cell line, derived from the ovary of Largemouth Bass Micropterus salmoides, is described. Gonad tissue was collected from Largemouth Bass that were electrofished from Oneida Lake, New York. The tissue was processed and grown in culture flasks at approximately 22°C for more ...
Zhang Xiaojun X a Aquatic Microbiology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences , Auburn University , Auburn , Alabama 36849 , - - 2014
Abstract The survival of Aeromonas hydrophila under low-nutrient conditions was investigated in this study. The behavior of three strains isolated from Common Carp Cyprinus carpio (China) and Channel Catfish Ictalurus punctatus (USA) was compared when cells were starved at different temperatures (4, 15, 25, and 35°C) over a 4-week period. ...
Fujikura Ushio U Institut für Biochemie und Biologie, Universität Potsdam, Potsdam-Golm, - - 2014
Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in ...
Libby Eric E Santa Fe Institute, Santa Fe, New Mexico, United States of - - 2014
Organisms have increased in complexity through a series of major evolutionary transitions, in which formerly autonomous entities become parts of a novel higher-level entity. One intriguing feature of the higher-level entity after some major transitions is a division of reproductive labor among its lower-level units in which reproduction is the ...
Sonal Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, - - 2014
The epidermis is a stratified epithelium, which forms a barrier to maintain the internal milieu in metazoans. Being the outermost tissue, growth of the epidermis has to be strictly coordinated with the growth of the embryo. The key parameters that determine tissue growth are cell number and cell size. So ...
Gotovdorj Tuvshinjargal T 0000-0001-6174-2022 Molecular, Cellular and Developmental Biology, Division of Biomedical Science, Graduate School, Korea University, Seoul, Korea. ; Department of Preventive Medicine, College of Medicine, Korea University, Seoul, - - 2014
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can induce drug transporter genes such as the ATP-binding cassette G member 2 (ABCG2), which contributes to multidrug resistance. We investigated the effect of TCDD pretreatment on drug transporters induction from cancer cells of various origins. Cell viabilities after treatment of cisplatin were measured to evaluate acquiring cisplatin ...
Zhao Ya Ning YN Department of Pediatric Hematology and Immunology, West China Second University Hospital, Sichuan University. Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, Sichuan, China; Department of Phymatology, Baoji Municipal Central Hospital, Baoji 721008, Shaanxi, - - 2014
To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biological processes of BIM activation so as to reverse the chemoresistance of cancer cells. BIMS was specifically amplified from HL-60 cells by RT-PCR, confirmed to be correct by sequencing and cloned into shuttle vector pAdTrack-CMV carrying a green fluorescence ...
Roomi M W MW Dr. Rath Research Institute, Santa Clara CA-95035, - - 2014
Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metastasis. Biological agents that prevent extracellular matrix ...
Chekhun S V SV R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, - - 2014
The aim of our study is to investigate the disorders of ferritin functioning in breast cancer (BC) cells of different molecular subtype. The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A, and 184A1. Ferritin heavy chains (FTH) expression was studied by immunohistochemical method. "Free iron" content ...
Zhuravel O V OV R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, - - 2014
The study was aimed on analysis of human beta-defensin-1-4 (hBDs) mRNA expression in cultured thyroid cancer cells and evaluation of effects of recombinant hBD-2 (rec-hBD-2) on growth patterns, migration properties and expression of E-cadherin and vimentin in these cells. The study was performed on cultured follicular thyroid cancer WRO cells, ...
Tan J-K JK School of Medicine, International Medical University, Bukit Jalil, Kuala Lumpur 57000, - - 2014
Deregulation of microRNA has been associated with cancer progression and the modification of cancer phenotypes could be achieved by targeting microRNA expression. This study aimed to determine the effects of miR-372 on cell progression and gene expression in nasopharyngeal carcinoma cell line, TW01. NPC TW01 cells were transfected with the ...
Li Xiaofang X Department of Gastroenterology, Henan Provincial People's Hospital, Zhengzhou, - - 2014
The research protocol is MTT (Methyl ThiazolylTetrazolium) method, Hoechst33342 staining method and flow cytometry detection to observe the effect of minor bupleurum decoction on proliferation inhibition and apoptosis-inducing of esophageal cancer cell strain Eca-109 cell and its purpose is to discuss the effect. The result of MTT method shows that ...
Hui Wu W Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, P.R. - - 2014
Apply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hhsignaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and inductionof apoptosis. Utilizing SmosiRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and Western blot technology to detect Smo, Gli1 mRNA and protein expression in each group ...
Zhang Yong Y Department of Pathology, Medical School of Jinan University, Guangzhou, 510632, People's Republic of - - 2014
Rational: Combination therapy to inhibit cancer stem cells may have important clinical implications. Here, we examine the molecular mechanisms by which epigallocatechin gallate (EGCG), a bioactive polyphenol in green tea, inhibits the stem cell characteristics of glioma stem-like cells (GSLCs) and synergizes with temozolomide (TMZ), a DNA-methylating agent commonly used ...
Tsuruya Makoto M Tohoku University Graduate School of Dentistry, 4-1 Seiryo-Machi, Aoba-ku, Sendai, 980-8575, - - 2014
Under the hypothesis that photo-irradiated proanthocyanidin could accelerate wound healing through reactive oxygen species (ROS) formation, we examined the effect of proanthocyanidin on 3T3-L1 mouse fibroblasts with or without photo-irradiation. As a result, irrespective of presence or absence of photo-irradiation, only 1 min exposure of the cells to proanthocyanidin resulted in ...
Prater Michael D MD Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, - - 2014
Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin-myosin interactions, and on average, 65% ...
Oh Bea Jun BJ Division of Endocrinology and Metabolism, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of - - 2014
Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature ...
Gen-Huan Yang Y Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing - - 2014
Objective To establish a stable cell line overexpression heme oxygenase-1(HO-1) mediated by a modified lentivirus system and identify its function. Methods The HO-1 gene was amplified by polymerase chain reaction and cloned into the modified pLentiLox3.7 expression vectors. The recombinant plasmids were transfected into HEK293T cells and the HO-1 was ...
Vaid Mudit M Department of Dermatology, University of Alabama at Birmingham, Birmingham, - - 2014
Cutaneous malignant melanoma is the leading cause of death from skin diseases and is often associated with activating mutations of the proto-oncogene BRAF. To develop more effective strategies for the prevention or treatment of melanoma, we have examined the inhibitory effects of silymarin, a flavanoid from Silybum marianum, on melanoma ...
Higuchi Masaya M Division of Virology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, - - 2014
Human T-cell leukemia virus type 1 (HTLV-1), an etiological agent of adult T-cell leukemia, immortalizes and transforms primary human T cells in vitro in both an interleukin (IL)-2-dependent and IL-2-independent manner. Expression of the HTLV-1 oncoprotein Tax transforms the growth of the mouse T-cell line CTLL-2 from being IL-2-dependent to ...
Wan Shanshan S Department of Surgery, University of Michigan, Ann Arbor, - - 2014
& Aims: Cancer stem cells (CSCs) can contribute to hepatocellular carcinoma (HCC) progression and recurrence following therapy. The presence of tumor-associated macrophages (TAMs) in patients with HCC is associated with poor outcomes. It is not clear whether TAMs interact with CSCs during HCC development. We investigated whether TAMs affect the ...
Fofaria Neel M NM Department of Biomedical Sciences & Cancer Biology Center, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, - - 2014
Anoikis is an anchorage-independent cell death. Resistance to anoikis is one of the key features of metastatic cells. Here, we analyzed the role of STAT3 in anoikis resistance in melanoma cells leading to metastasis. When grown under anchorage-independent conditions, significant proportion of cells resisted anoikis and these resistant cells had ...
Bisteau Xavier X - - 2014
Comment on: Lubanska D and Porter LA. The atypical cell cycle regulator Spy1 suppresses differentiation of the neuroblastoma stem cell population. Oncoscience. 2014; 1: 336-348.
Shulman Ziv Z Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, - - 2014
T follicular helper (T(FH)) cells select high-affinity, antibody-producing B cells for clonal expansion in germinal centers (GCs), but the nature of their interaction is not well defined. Using intravital imaging, we found that selection is mediated by large but transient contacts between T(FH) and GC B cells presenting the highest ...
Greife Annemarie - - 2014
Notch signalling regulates cell fate in most tissues, promoting precursor cell proliferation in some, but differentiation in others. Accordingly, downregulation or overactivity variously contributes to cancer development. So far, little is known about Notch pathway activity and function in the normal urothelium and in urothelial carcinoma (UC). We have therefore ...
Takahashi Y Y The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Hirakata, - - 2014
The stem cell compartment in the esophageal epithelium is possibly located in the basal layer. We have identified significant expression of Smad2/3, phosphorylated at specific linker threonine residues (pSmad2/3L-Thr), in the epithelial cells of murine stomach and intestine, and have suggested that these cells are epithelial stem cells. In this ...
Reichl Maren R - - 2014
The complexity of biology requires that measurements of biomolecular interactions have to be performed inside the living cell. While electrophoresis is prohibited by the cell membrane inside cells, the movement of molecules along a temperature gradient appears feasible. This thermophoresis could recently quantify binding affinities in vitro at picomolar levels ...
Lin Li-Chiung LC Department of Life Sciences, National Chung Hsing University, Taichung, - - 2014
Signaling pathway(s) responsible for transforming growth factor β (TGFβ)-induced epithelial mesenchymal transition (EMT), invasion and migration of H460 cells (non-small cell lung cancer/NSCLC) was identified in the study. The results showed that TGFβ-induced p(38)/β-catenin/PPARγ signaling pathway played a critical role in the promotion of EMT, invasion and migration of H460 ...
Xiao Li L Department of Pharmacology, The Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-0071, - - 2014
During embryonic development, cell death transforms the solid embryonic cell mass into a hollow structure (cavitation) which allows the surviving cells to differentiate into varied tissues and organs around the cavity. This process can be partly reproduced with embryonic stem cells. However, it is unknown if adult stem cell masses ...
Stoddart Martin J MJ AO Research Institute Davos, 7270 Davos Platz, Switzerland. Electronic address: - - 2014
Regenerative medicine approaches to cartilage tissue repair have mainly been concerned with the implantation of a scaffold material containing monolayer expanded cells into the defect, with the aim to differentiate the cells into chondrocytes. While this may be a valid approach, the secretome of the implanted cells and its effects ...
Penzo-Méndez Alfredo I AI Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, - - 2014
The study of animal organ size determination has provided evidence of the existence of organ-intrinsic mechanisms that 'sense' and adjust organ growth. Cell competition, a form of cell interaction that equalizes cell population growth, has been proposed to play a role in organ size regulation. Cell competition involves a cell-context ...
Li Jianying J Department of Respiratory Disease, Affiliated Xi'an Central Hospital, Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710003, China; Center of Cancer Research, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710003, - - 2014
β-Elemene, a novel antitumor plant drug extracted from the traditional Chinese medicinal herb Zedoary, has been shown to be effective against a wide variety of tumors. Recent studies have indicated that β-elemene can inhibit the growth of lung cancer cells; however, the exact mechanism of β-element's action in lung cancer ...
Valsechi Marina Curado - - 2014
Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute ...
Calles Antonio A Lowe Center for Thoracic Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, - - 2014
MET targeted therapies are under clinical evaluation for non-small-cell lung cancer (NSCLC) patients. Tyrosine kinase inhibitors (TKI) against MET have varying degrees of specificity. Tivantinib (ARQ 197) is reported to be a non-ATP competitive selective MET inhibitor. We aimed to compare the activity of tivantinib to established MET TKIs in ...
Sauter D R P DR Section for Cell and Developmental Biology, Department of Biology, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Ø, Denmark, - - 2014
Pancreatic ductal adenocarcinoma (PDAC) has one of the worst survival rates of all cancers. ANO1 (TMEM16A) is a recently identified Ca(2+)-activated Cl(-) channel (CaCC) that is upregulated in several tumors. Although ANO1 was subject to extensive studies in the recent years, its pathophysiological function has only been poorly understood. The ...
Takemoto Kenji K Department of Applied Biological Science, Tokyo University of Science , Noda, Chiba 278-8510, - - 2014
New dihydronaphthoquinone derivatives, karuquinone A (1), karuquinone B (2), and karuquinone C (3), were isolated from a fungal culture broth of Fusarium solani. The structures were determined by interpretation of spectroscopic data (1D/2D NMR, MS, and IR). Three known compounds, javanicin (4), 2,3-dihydro-5-hydroxy-8-methoxy-2,4-dimethylnaphtho[1,2-b]furan-6,9-dione (5), and 5-hydroxydihydrofusarubin C (6), were also ...
Fernandes João Tiago S JT INESC Microsistemas e Nanotecnologias and IN - Institute of Nanoscience and Nanotechnology, R. Alves Redol, 9, 1000-029, Lisbon, - - 2014
Parkinson's disease (PD) is a common age-associated neurodegenerative disorder. The protein α-synuclein (aSyn) is a key factor in PD both due to its association with familial and sporadic cases and because it is the main component of the pathological protein aggregates known as Lewy bodies. However, the precise cellular effects ...
Shahneh Fatemeh Zare - - 2014
Background: Prostate cancer is the second leading malignancy worldwide and the second prominent cause of cancer-related deaths among men. Therefore, there is a serious necessity for finding advanced alternative therapeutic measures against this lethal malignancy. In this article, we report the cytotoxicity and the mechanism of cell death of the ...
Hsiao Pei-Ching PC School of Medicine, Chung Shan Medical University, Taichung, - - 2014
Nobiletin, a compound isolated from citrus fruits, is a polymethoxylated flavone derivative that was shown to have anti-inflammatory and anticancer activities in various solid tumors. The anticancer effect of nobiletin on nonsolid tumor remains unclear. Herein, the molecular mechanisms by which nobiletin exerts its anticancer effects on acute myeloid leukemia ...
Szwed Marzena - - 2014
Abstract In this study we focused on the evaluation of the pro-oxidant properties of doxorubicin - transferrin (DOX-TRF) conjugate and its potency to damage macromolecules which are components of cellular compartments. Our experiments were performed on two human leukemia cell lines: K562 (chronic erythromyeloblastoid leukemia) and CCRF-CEM (acute lymphoblastic leukemia). ...
Su Huimin H Key Laboratory of Education Ministry of China for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, 010021, China, - - 2014
Sertoli cells have important functions in the testis for spermatogenesis. Thus, Sertoli cell culture systems have been established in many animals, such as rat, mouse, human, dog, cow, and pig, but a goat culture has not been reported. This study describes the isolation and culture of Sertoli cells from 3- ...
Jambhrunkar Siddharth - - 2014
Mesoporous silica nanoparticles (MCM-41) with different surface chemistry were used as carrier system to study its influence on drug delivery and anticancer activity of curcumin (CUR). CUR was encapsulated in pristine MCM-41 (hydrophilic and negatively charged), amino functionalized MCM-41 (MCM-41-NH2 which is hydrophilic and positively charged) and methyl functionalized MCM-41 ...
Zhang Yu-Long YL Yu-Long Zhang, You-Cheng Zhang, Wei Han, Yu-Min Li, Geng-Nian Wang, Shao Yuan, Feng-Xian Wei, Jia-Feng Wang, Jian-Jun Jiang, Ya-Wu Zhang, Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, - - 2014
To investigate the roles of Golgi protein (GP) 73 in the regulation of cell proliferation and apoptosis. Stealth RNAi targeting GP73 gene sequence was used to silence its expression in Hep G2 cells and Bel7402 cells. Stealth RNAi effects were assessed by reverse transcriptase polymerase chain reaction and ELISA. Cell ...
Jorge Patrícia Mendes PM Departamento de Biofísica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, - - 2014
The diphenyl ditelluride (DPDT) is a prototype for the development of new biologically active molecules. In previous studies, DPDT showed an elevated cytotoxicity in Chinese hamster fibroblast (V79) cells but the mechanisms for reduction of cell viability still remain unknown. DPDT showed mutagenic properties by induction of frameshift mutations in ...
Almeida Afonso R M AR Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisboa, - - 2014
T helper (Th) cells are critical players in the modulation of immune response outcomes. Activation of Th cells gives rise to various subsets of effector cells that are controlled via specialized regulatory T cells or through self-regulation via production of IL-10. However, the environmental factors that regulate IL-10 production by ...
< 3 4 5 6 7 8 9 10 11 12 13 >