Search Results
Results 301 - 350 of 2093
< 2 3 4 5 6 7 8 9 10 11 12 >
Andarawewa Kumari L KL Department of Radiation Oncology; Robert M. Berne Cardiovascular Research - - 2014
We observed that in human M21 melanoma cells, chemotherapy was effective with adherent cells yet suspended cells were highly resistant [1]. Moreover, in 4/5 randomly chosen primary melanoma isolates, cell detachment induced chemoresistance. These results are of particular relevance because melanomas typically grow in densely packed cellular "nests" that contain ...
Liang Jun J Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huai-hai Road, Xuzhou, Jiangsu, 221002, PR China; Lab of Neurosurgery, Xuzhou Medical College, Xuzhou, Jiangsu, - - 2014
Bmi-1 is involved in the development of several human cancers, however, the its significance in glioma progression remains largely unknown. We report that downregulation of Bmi-1 clearly reduces glioma cell migration and invasion. Downregulation of Bmi-1 promotes the expression of the tumor suppressor p16, which is important in glioma cell ...
Hefei Wang W National Research Center for Animal Transgenic Bio-technology, Inner Mongolia University, 24 Zhao Jun Road, Hohhot, 010070, - - 2014
Diabetes is a significant public health problem that can be treated with insulin therapy; however, therapies designed to cure diabetes are limited. The goal of the current study was to assess the potential for curative treatment of diabetes using adipose-derived stem cells (ADSCs). To achieve this goal, the differentiation of ...
He Yongchun Y Laboratory of Oral Biomedical Science and Translational Medicine, Department of Orthodontics, School of Stomatology, Tongji University, Shanghai, 200072, - - 2014
Tumor protein D52-like 2 (TPD52L2) and its family members form homo- and heteromeric complexes essential for cell proliferation in multiple human cancers. TPD52L2 is involved in cell migration and attachment in oral squamous cell carcinoma (OSCC). To confirm the role of TPD52L2 in OSCC, we employed the lentivirusdelivered small interfering ...
Wu Qiuge Q Department of respiratory and cwritical care medicine, the first affiliated hospital of Zhengzhou - - 2014
Hispolon is isolated from P. igniarius and exhibits antitumor activity. Here, we explored the effects of hispolon on the lung cancer A549 and H661 cells. Cells were incubated with various concentrations of hispolon (0, 5, 10, 20, 40, 80 or 160 μM) for 12, 24, 48 or 72 h. Cell ...
Bellas Evangelia E Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States; The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, United - - 2014
Cells change their shape and mechanics dramatically during development and tissue healing in response to morphogens, cell-cell contact, adhesion to extracellular matrix, and more. Several regulatory links have been described between cell shape, cytoskeletal tension, matrix adhesiveness and stiffness, and recent studies have begun to uncover how these mechanotransduction pathways ...
Naumenko Ekaterina A EA Bionanotechnology Lab, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russian - - 2014
Nanotechnology offers an unprecedented number of opportunities for biomedical research, utilizing the unusual functionalities of nanosized materials. Here we describe the recent advances in fabrication and utilization of nanoparticle-labelled cells. We present a brief overview of the most promising techniques, namely layer-by-layer polyelectrolyte assembly on cells and intracellular and extracellular ...
Hashimoto Noriaki - - 2014
Cancer stem cells (CSCs) are thought to play important roles in therapy-resistance. In this study, we induced cancer stem-like cells from hepatocellular carcinoma (HCC) cell lines using a unique medium, and examined their potential for resistance to anti-cancer drugs. The human HCC cell lines SK-HEP-1 (SK), HLE, Hep 3B, and ...
Kim Kyong K Research Group of Food Functionality, Division of Creative Food Science for Health, Korea Food Research Institute , Sungnam-si, - - 2014
Abstract Spergularia marina Griseb. (SM) is a halophyte that grows in mud flats. The aerial portions of SM have been eaten as vegetables and traditionally used to prevent chronic diseases in Korea. However, there has been no scientific report that demonstrates the pharmacological effects of SM. Glucagon-like peptide-1 (GLP-1) is ...
Tong Feifei - - 2014
A new multi-channel series piezoelectric quartz crystal (MSPQC) cell sensor for real time monitoring of living cells in vitro was reported in this paper. The constructed sensor was used successfully to monitor adhesion, spreading, proliferation, and apoptosis of MG63 osteosarcoma cells and investigate the effects of different concentrations of cobalt ...
Nascimento Ana Vanessa AV CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, IINFACTS, Rua Central de Gandra 1317, 4585-116 Gandra PRD, - - 2014
RNA interference has emerged as a powerful strategy in cancer therapy because it allows silencing of specific genes associated with tumor progression and resistance. Mad2 is an essential mitotic checkpoint component required for accurate chromosome segregation during mitosis, and its complete abolition leads to cell death. We have developed an ...
Liu Boyin B Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, - - 2014
We report a novel approach to probe the interior of single bacterial cells at nanometre resolution by combining focused ion beam (FIB) and atomic force microscopy (AFM). After removing layers of pre-defined thickness in the order of 100 nm on the target bacterial cells with FIB milling, AFM of different ...
Kanafi Mohammad Mahboob MM School of Regenerative Medicine, Manipal University, Bangalore, 560065, - - 2014
To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single ...
Silva Carolina Amália Barcellos CA Department of Oral Pathology - - 2014
Abstract Myoepithelial cells play a central role in glandular tumors, regulating the progression of in situ to invasive neoplasias, with the tumor microenvironment being shown to be involved in both initiation and progression. This study aimed to analyze the in vitro effects of fibroblast growth factor-2 (FGF-2) and hepatocyte growth ...
Ravid-Hermesh Orit O The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Department of Physics Building 214, Bar-Ilan University, Ramat Gan, 5290002, - - 2014
Cell populations represent intrinsically heterogeneous systems with a high level of spatiotemporal complexity. Monitoring and understanding cell-to-cell diversity is essential for the research and application of intra- and interpopulation variations. Optical analysis of live cells is challenging since both adherent and nonadherent cells change their spatial location. However, most currently ...
Liu Y Y 1] Center for Autophagy Research, University of Texas Southwestern Medical Center, Dallas, TX 75390-9113, USA [2] Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9113, - - 2014
It is controversial whether cells truly die via autophagy or whether - in dying cells - autophagy is merely an innocent bystander or a well-intentioned 'Good Samaritan' trying to prevent inevitable cellular demise. However, there is increasing evidence that the genetic machinery of autophagy may be essential for cell death ...
Lee Sooncheol S Center for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA - - 2014
Proliferation arrest and distinct developmental stages alter and decrease general translation yet maintain ongoing translation. The factors that support translation in these conditions remain to be characterized. We investigated an altered translation factor in three cell states considered to have reduced general translation: immature Xenopus laevis oocytes, mouse ES cells, ...
Ishida Takashi T Graduate School of Science and Technology, Kumamoto University, Kumamoto, - - 2014
Cell-to-cell communication is a fundamental mechanism for coordinating developmental and physiological events in multicellular organisms. Heterotrimeric G proteins are key molecules that transmit extracellular signals; similarly, CLAVATA signaling is a crucial regulator in plant development. Here, we show that Arabidopsis thaliana Gβ mutants exhibit an enlarged stem cell region, which ...
Matsumura Taku T Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, - - 2014
The microenvironment of cells, which includes basement proteins, shear stress, and extracellular stimuli, should be taken into consideration when examining physiological cell behavior. Although microfluidic devices allow cellular responses to be analyzed with ease at the single-cell level, few have been designed to recover cells. We herein demonstrated that a ...
Lan Thanh T Ha TT Departments of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, 48109, - - 2014
Cutaneous spindle cell malignancies such as sarcomatoid squamous cell carcinoma (SCC), leiomyosarcoma, desmoplastic melanoma (DM), and atypical fibroxanthoma (AFX) may be morphologically indistinguishable, yet accurate diagnosis is important for appropriate clinical management. The distinction amongst these entities relies on immunohistochemical evaluation for epidermal, muscle, or melanocytic differentiation. Epidermal differentiation markers ...
Roubinet Chantal C Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, - - 2014
Asymmetric cell division (ACD) is a mechanism to generate cellular diversity and used by prokaryotes and eukaryotes alike. Stem cells in particular rely on ACD to self-renew the stem cell while simultaneously generating a differentiating sibling. It is well established that the differential partitioning of cell fate determinants in the ...
Lee Sora S Department of Pediatric Surgery and Cancer Biology, Vanderbilt University Medical Center, Nashville, TN - - 2014
Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft ...
Qi Xin-Hui XH Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. - - 2014
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death in the world. The gene glypican-3 (GPC3) is reported to be a potential therapeutic target for HCC. In this study, we use RNA interference with lentiviral vectors to explore the effect of GPC3 silencing on the biological behavior of ...
Zhang Jihong J Faculty of Medicine, Kunming University of Science and Technology, Kunming, - - 2014
Glioblastoma multiforme (GBM) treatment includes temozolomide (TMZ) chemotherapy. O6-Methylguanine lesions are repaired by methylguanine-DNA methyltransferase (MGMT). Response to TMZ requires low MGMT and functional mismatch repair (MMR); resistance, conferred by MGMT or MMR deficiency, represents a barrier to successful treatment. TMZ analogs were synthesized, substituting N3-methyl with propargyl ( 1 ...
Nowacki Laetitia L FRE CNRS 3580, Génie Enzymatique et Cellulaire, Université de Technologie de Compiègne, Centre de Recherches de Royallieu, CS 60319, 60203 Compiègne, France; UMR CNRS 7338, BioMécanique et BioIngénierie, Université de Technologie de Compiègne, Centre de Recherches de Royallieu, CS 60319, 60203 Compiègne, - - 2014
Since a few years, the acoustic sensing of whole cell is the focus of increasing interest for monitoring the cytoskeletal cellular response to morphological modulators. We aimed at illustrating the potentialities of the quartz crystal microbalance with dissipation (QCM-D) technique for the real-time detection of the earliest morphological changes that ...
Ringer Lymor L Department of Oncology and Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA. Contributed equally to this - - 2014
The p53 tumor suppressor protein plays a crucial role in influencing cell fate decisions in response to cellular stress. As p53 elicits cell cycle arrest, senescence or apoptosis, the integrity of the p53 pathway is considered a key determinant of anti-tumor responses. p53 can also promote autophagy, however the role ...
Chen Dongdong - - 2014
Glioblastoma multiforme (GBM) is very difficult to treat with conventional anti-cancer/anti-apoptotic drugs. We tested the hypothesis that inhibition of Na+/K+-ATPase causess a mixed or hybrid form of concurrent apoptosis and necrosis and therefore should enhance anti-cancer effects of chemotherapy on glioblastoma cells. In human LN229 and drug-resistant T98G glioblastoma cell ...
Wang Chunlei - - 2014
BackgroundAmong glioma treatment strategies, arsenic trioxide (As2O3) has shown efficacy as a therapeutic agent against human gliomas. However, the exact antitumor mechanism of action of As2O3 is still unclear. Mitochondria are considered to be the major source of intracellular reactive oxygen species (ROS), which are known to be associated with ...
Choi Hyong Woo HW Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of - - 2014
Pepper (Capsicum annuum L.) provides a good experimental system for studying the molecular and functional genomics underlying the ability of plants to defend themselves against microbial pathogens. Cell death is a genetically programmed response that requires specific host cellular factors. Hypersensitive response (HR) is defined as rapid cell death in ...
Camley Brian A BA Departments of Physics and Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, CA 92093; Departments - - 2014
Pairs of endothelial cells on adhesive micropatterns rotate persistently, but pairs of fibroblasts do not; coherent rotation is present in normal mammary acini and kidney cells but absent in cancerous cells. Why? To answer this question, we develop a computational model of pairs of mammalian cells on adhesive micropatterns using ...
Vashi Aditya V AV CSIRO Manufacturing Flagship, Clayton, 3169, - - 2014
The present study has evaluated a commercial pericardial material for its capacity to assist as a natural extracellular matrix patch for the delivery and retention of mesenchymal stem cells for cardiac repair. The repair of cardiac tissue with cells delivered by an appropriate bioscaffold is expected to offer a superior, ...
Zhou Jianya J Department of Respiratory Disease, Thoracic Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University,, Hangzhou, - - 2014
To evaluate the clinical value of cell block from malignant pleural effusion (MPE) as an alternative sample versus tumor tissue for anaplastic lymphoma kinase (ALK) detection in patients with advanced non small cell lung cancer (NSCLC). Fifty-two matched samples were eligible for analysis. ALK status was detected by Ventana IHC ...
Maggioni Daniele D a Department of Surgery and Translational Medicine , University of Milan-Bicocca , Monza , - - 2014
In this study the potential anticancer effect of 2 flavonoids, myiricetin (MYR) and naringenin (NAR) has been evaluated on an oral squamous cell carcinoma (OSCC) cell line, SCC-25, and HaCaT cells. Both the flavonoids inhibited SCC-25 cell growth, although NAR selectively affected cancer cells without impairing HaCaT cell growth. The ...
Li Min M Department of Ophthalmology, Shanghai Tenth People's Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai, 200072, P.R. - - 2014
The role of microRNA (miRNA) in proliferative vitreoretinopathy (PVR) progression has not been studied extensively, especially in retinal pigment epithelial-mesenchymal transition (EMT) which is the main reason for formation of PVR. In this study, we first investigated the miRNA expression profile in transforming growth factor beta 1 (TGF-β1) mediated EMT ...
Ma Ji J Central Laboratory, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. - - 2014
microRNAs (miRNAs), as gene expression regulators, have been identified to be closely associated with tumorigenesis. Thus a loss-of-function study is more likely to reveal the biological roles of endogenous miRNAs. Genetic knockout, antisense oligonucleotide inhibitors, and miRNA sponge (miR‑SP) are usually performed to inhibit the activities of miRNAs of interest. ...
Semmler Judith J Institute of Neurophysiology, University of Cologne, Cologne, - - 2014
Background/Aims: In vitro reprogramming of somatic cells holds great potential to serve as an autologous source of cells for tissue repair. However, major difficulties in achieving this potential include obtaining homogeneous and stable cells for transplantation. High electrical activity of cells such as cardiomyocytes (CMs) is crucial for both, safety ...
Cheng Xinlai - - 2014
Cancer cells in the advanced stage show aberrant antioxidant capacity to detoxify excessive ROS resulting in the compensation for intrinsic oxidative stress and therapeutic resistance. PDAC is one of the most lethal cancers and often associated with a high accumulation of ROS. Recent studies identified gold(I) NHC complexes as potent ...
Hill Jacqueline E - - 2014
We have examined a series of selenorhodamine derivatives with amide and thioamide functionality (N,N-diethyl and N-piperidyl) at the 5-position of a 9-(2-thienyl) substituent on the selenorhodamine core for their potential as photosensitizers for photodynamic therapy (PDT) in P-glycoprotein (P-gp)-expressing cells. These compounds were examined for their photophysical properties (absorption, fluorescence, ...
Son Jang-Ho JH Department of Oral and Maxillofacial Surgery, Ulsan University Hospital, College of Medicine, Ulsan University, Ulsan, South - - 2014
Osteoblastic differentiation and bone forming capacity are known to be suppressed under hypoxic conditions. Melatonin has been shown to influence cell differentiation. A number of in vitro and in vivo studies have suggested that melatonin also has an anabolic effect on bone, by promoting osteoblastic differentiation. However, the precise mechanisms ...
Maeda Junko J a  Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, 80523; - - 2014
Potentially lethal damage (PLD) repair has been defined as that property conferring the ability of cells to recover from DNA damage depending on the postirradiation environment. Using a novel cyclin dependent kinase 1 inhibitor RO-3306 to arrest cells in the G2 phase of the cell cycle, examined PLD repair in ...
Wei Zewen - - 2014
Microfluidics based continuous cell electroporation is an appealing approach for high-throughput cell transfection, but cell viability of existing methods as such is usually compromised by adverse electrical or hydrodynamic effects. Here we present the validation of a flow-through cell electroporation microchip, in which dielectrophoretic force was employed to sort viable ...
Cai Erica P EP Toronto General Research Institute, University Health Network, 101 College Street, MaRS Centre/TMDT, Room 10-363, Toronto, ON, M5G 1L7, - - 2014
Diabetes mellitus is characterised by beta cell loss and alpha cell expansion. Analogues of glucagon-like peptide-1 (GLP-1) are used therapeutically to antagonise these processes; thus, we hypothesised that the related cell cycle regulators retinoblastoma protein (Rb) and p107 were involved in GLP-1 action. We used small interfering RNA and adenoviruses ...
Dełeńko Konrad K Department of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland, - - 2014
The mechanisms of plant cell dedifferentiation and the acquisition of totipotency are poorly understood. One of the methods to induce the dedifferentiation process in plant cells is simple and requires the removal of the cell wall. After cell wall removal in protoplasts, large-scale chromatin decondensation is observed (Tessadori et al. ...
Zhao Jing-Ya JY Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China. - - 2014
Gold nanoclusters (AuNCs) with ultra small sizes and unique fluorescence properties have shown promising potential for imaging the nuclei of living cells. However, little is known regarding the potential cytotoxicity of AuNCs after they enter the cell nucleus. The aim of this study is to investigate whether and how nucleus-targeting ...
Quattrocolo Giulia G Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois - - 2014
Cajal-Retzius cells orchestrate the development of cortical circuits by secreting the glycoprotein reelin. However, their computational functions are still unknown. In fact, the nature of their postsynaptic targets, major neurotransmitter released, as well as the class of postsynaptic receptors activated by their firing remain unclear. Here, we have addressed these ...
Peluso John J - - 2014
Progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 are expressed in rat granulosa cells and spontaneously immortalized granulosa cells (SIGCs) but their biological roles are not well defined. The present studies demonstrate that depleting either Pgrmc1 or Pgrmc2 in SIGCs increases entry into the cell cycle but does not increase ...
Rao Divya K DK University of Michigan, United - - 2014
This paper introduces a strategy to kill selectively multidrug resistant cells that express the ABCG2 transporter (also called breast cancer resistance protein, BCRP). The approach is based on specific stimulation of ATP hydrolysis by ABCG2 transporters with sub-toxic doses of curcumin combined with stimulation of ATP hydrolysis by the Na+ ...
Takahashi Saori S Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, - - 2014
The recent successful establishment of mouse parthenogenetic haploid embryonic stem cells (phESCs) and androgenetic haploid ESCs (ahESCs) has stimulated genetic research not only in vitro but also in vivo because of the germline competence of these cell lines. However, it is difficult to maintain the haploid status over time without ...
Riesco Marta F - - 2014
The possibility of generating Primordial Germ Cells (PGCs) in vitro from non-committed embryonic cells represents an extremely useful tool in current research. PGC in vitro differentiation has been successfully reported in mammals. However, contrary to fish, PGC specification in mammals is an inductive mechanism. This study is the first to ...
Shrestha Gajendra G Department of Biology, Brigham Young University, Provo, UT, USA; M. L. Bean Life Science Museum, Brigham Young University, Provo, UT, - - 2014
Cancer is the second leading cause of human deaths in the USA. Despite continuous efforts to treat cancer over the past 50 years, human mortality rates have not decreased significantly. Natural products, such as lichens, have been good sources of anticancer drugs. This study reports the cytotoxic activity of crude extracts ...
< 2 3 4 5 6 7 8 9 10 11 12 >