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Results 301 - 350 of 1466
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Huijskens Mirelle J A J MJ Department of Internal Medicine, Division of Hematology, Maastricht University Medical Center, and Department of Toxicogenomics, School for Oncology and Developmental Biology, Maastricht University, Maastricht, the Netherlands - - 2014
The efficacy of donor HSCT is partly reduced as a result of slow post-transplantation immune recovery. In particular, T cell regeneration is generally delayed, resulting in high infection-related mortality in the first years post-transplantation. Adoptive transfer of in vitro-generated human T cell progenitors seems a promising approach to accelerate T ...
Bird Sara Whitney SW Departments of Microbiology and Immunology - - 2014
The cell-to-cell spread of cytoplasmic constituents such as nonenveloped viruses and aggregated proteins is usually thought to require cell lysis. However, mechanisms of unconventional secretion have been described that bypass the secretory pathway for the extracellular delivery of cytoplasmic molecules. Components of the autophagy pathway, an intracellular recycling process, have ...
Fouchard Jonathan J Laboratoire Matière et Systèmes Complexes, Unité Mixte de Recherche 7057, Centre National de la Recherche Scientifique and Université Paris-Diderot, 75205 Paris, - - 2014
Cell shape affects proliferation and differentiation, which are processes known to depend on integrin-based focal adhesion (FA) signaling. Because shape results from force balance and FAs are mechanosensitive complexes transmitting tension from the cell structure to its mechanical environment, we investigated the interplay between 3D cell shape, traction forces generated ...
Gibellini Lara L Department of Surgery, Medicine, Dentistry, and Morphological Sciences, and Department of Life Sciences, University of Modena and Reggio Emilia, Modena, - - 2014
Lon is a nuclear-encoded, mitochondrial protease that assists protein folding, degrades oxidized/damaged proteins, and participates in maintaining mtDNA levels. Here we show that Lon is up-regulated in several human cancers and that its silencing in RKO colon cancer cells causes profound alterations of mitochondrial proteome and function, and cell death. ...
de Oliveira Alane Cabral AC Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceio, Al, 57072-970 (Brazil); Faculdade de Nutrição, Universidade Federal de Alagoas - - 2014
The aim of this work was to investigate the mechanism of action of 2-ferrocenyl-1,1-diphenylbut-1-ene (1) on HL-60 human leukemia cells. While inactive against noncancerous cells, 1 provoked a concentration-dependent decrease in viable tumor cells, primarily via apoptosis, as evidenced by analysis of cell morphology, activation of caspases 3 and 7, increased ...
Shalhout Sophia S Department of Chemistry, Wayne State University, Detroit, MI 48202, - - 2014
AID converts DNA-cytosines to uracils in immunoglobulin genes, creating antibody diversification. It also causes mutations and translocations that promote cancer. We examined the interplay between uracil creation by AID and its removal by UNG2 glycosylase in splenocytes undergoing maturation, and in B-cell cancers. The genomic uracil levels remain unchanged in ...
Keeley Patrick W PW Neuroscience Research Institute and Department of Psychological and Brain Sciences, University of California at Santa Barbara, Santa Barbara, CA - - 2014
Programmed cell death contributes to the histogenesis of the nervous system, and is believed to be modulated through the sustaining effects of afferents and targets during the period of synaptogenesis. Cone bipolar cells undergo programmed cell death during development, and we confirm that the numbers of three different types are ...
Walia Rupali R Faculty of Veterinary Medicine, University of Calgary, Alberta, - - 2014
Studies have shown that Porcine circovirus (PCV)-2 induces apoptosis in PK15 cells. Here we report that cell death is induced in PCV2b-infected PK15 cells that express Capsid (Cap) protein and this effect is enhanced in interferon gamma (IFN-γ)-treated cells. We further show that transient PCV2a and 2b-Cap protein expression induces ...
O'Brien Shalana S Department of Surgical Oncology; Roswell Park Cancer Institute; Buffalo, NY. University at Buffalo/State University of New York; Buffalo, - - 2014
Focal adhesion kinase (FAK) is up-regulated in thyroid cancer and small molecule FAK scaffolding inhibitor, Y15, was shown to decrease cancer growth in vitro and in vivo. We sought to test the effectiveness of Y15 in thyroid cancer cell lines, profile gene expression with Y15 compared with clinical trial FAK ...
Yang Xiaojing X Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu, 226001, People's Republic of - - 2014
Chromosome region maintenance 1 (CRM1) has been related to several malignancies. The predictive value of CRM1 in the malignance and prognosis of esophageal squamous cell carcinoma (ESCC), however, is not clear yet. In this study, we displayed that CRM1 expression was up-regulated in ESCC using immunohistochemistry and Western blot. Statistical ...
de Souza Ludmilla - - 2014
BackgroundSelol is an oily mixture of selenitetriacylglycerides that was obtained as a semi-synthetic compound containing selenite. Selol is effective against cancerous cells and less toxic to normal cells compared with inorganic forms of selenite. However, Selol¿s hydrophobicity hinders its administration in vivo. Therefore, the present study aimed to produce a ...
Pastorek M - - 2014
The aim of the present study was to compare the effect of realgar nanoparticles and arsenic trioxide (ATO) on viability, DNA damage, proliferation, autophagy and apoptosis in the human melanoma cell lines BOWES and A375. The application of various flow cytometric methods for measurements cell viability, DNA cell cycle, mitochondrial ...
Gao C - - 2014
Clear-cell renal cell carcinoma is a highly treatment-resistant tumor type. Heme oxygenase-1 plays an anti-apoptotic role in cancer chemotherapeutic inducing tumor-progression. The miR-200 family was involved in the process of mesenchymal-epithelial transition (MET) during renal development. In the present study, we demonstrated the regulatory relationship between miR-200c and HO-1. We provided ...
Pradhan Arun Kumar AK Academy of Scientific and Innovative Research, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India, - - 2014
Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The ...
Radojevic Vesna V Department of Biomedicine, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland, - - 2014
Somatostatin (SST) is a peptide hormone that exerts inhibitory effects mediated through binding to specific cell surface G protein-coupled receptors, of which five distinct subtypes (SSTR1-SSTR5) have been characterized. Our study performed on mouse cochlear hair cells shows the expression and localization of the three receptors (SSTR3-SSTR5) in wild-type (WT), ...
Aumsuwan Pranapda P National Center for Natural Product Research, School of Pharmacy, University of Mississippi, University, MS, 38677, - - 2014
The present study was designed to evaluate the efficacy of wild yam root extract (WYRE) as a potential demethylating agent using two breast cancer cell lines, MCF-7 (estrogen receptor positive; ER(+)) and MDA-MB-231 (Estrogen receptor negative; ER(-)), and a methylated gene, GATA3, as a potential marker of breast cancer development. ...
Wu Yuanhao Y Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, 200040, - - 2014
Although long non-coding RNAs (lncRNAs) are known to play an important role in cell regulation in several cancers, the regulatory mechanisms in renal carcinoma cells remain unclear. HOX transcript antisense RNA (HOTAIR), an lncRNA, coordinates with chromatin-modifying enzymes to regulate gene silencing. HOTAIR is over-expressed in several types of carcinoma ...
Martin Ciara A CA UCLA Interdepartmental Program in Molecular Toxicology. Electronic address: - - 2014
The neurodegenerative effects of Parkinson's disease (PD) are marked by a selective loss of dopaminergic (DA) neurons. Epidemiological studies suggest that chronic exposure to the pesticide paraquat may increase the risk for PD and DA cell loss. However, combined exposure with additional fungicide(s) including maneb and/or ziram may be required ...
Lee Chi-Chiu CC Department of Food Science and Center of Excellence for the Ocean, National Taiwan Ocean University. 2 Pei-Ning Road, Keelung 202, Taiwan, - - 2014
The crown-of-thorns starfish (Acanthaster planci) is a venomous starfish. In this study, the extraction of A. planci spine venom (ASV) was performed by phosphate saline buffer, followed by assaying the cytotoxicity on human normal and tumor cells. It was found that human melanoma cells (A375.S2) were the most sensitive to ...
Martins Alessandro F AF Grupo de Materiais Poliméricos e Compósitos, GMPC - Departamento de Química. Universidade Estadual de Maringá UEM - Av. Colombo 5790 - CEP 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR),-Estrada para Boa Esperança; CEP 85660-000, Dois Vizinhos, Paraná, Brasil. Electronic address: - - 2014
Polyelectrolyte complex beads based on N,N,N-trimethyl chitosan (TMC) and sodium alginate (ALG) were obtained. This biomaterial was characterised by FTIR, TGA/DTG, DSC and SEM analysis. The good properties of polyelectrolyte complex hydrogel beads were associated, for the first time, with gold nanoparticles (AuNPs). Through a straightforward methodology, AuNPs were encapsulated ...
Huang Chih-Ping CP Sex Hormone Research Center, Departments of Urology/Surgery and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, - - 2014
The urothelium is constantly rebuilt by normal urothelial cells to regenerate damaged tissues caused by stimuli in urine. However, the urothelial carcinoma cells expand the territory by aberrant growth of tumor cells, which migrate and occupy the damaged tissues to spread outside and disrupt the normal cells and organized tissues ...
Xue Zhongfeng Z The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 16, Jichang Road, 510405 Guangzhou, China; Sanya Traditional Chinese Medicine Hospital, 106, Fenghuang Road, 572000 Sanya, China. Electronic address: - - 2014
Alzheimer's disease (AD) is an age related and progressive neurodegenerative disease. Autophagy is a self-degradative process and plays a critical role in removing long-lived proteins and damaged organelles. Recent evidence suggests that autophagy might be involved in the pathogenesis of AD. β-asarone have various neuroprotective effects. However, the effect of ...
Pennington Elliot C EC Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, Mass., - - 2014
Purpose: The proportions of select stem cells in term amniotic fluid have been shown to correlate with the type and size of experimental neural tube defects (NTDs). We sought to determine the impact of gestational age upon this form of targeted amniotic cell profiling. Methods: Sprague-Dawley fetuses with retinoic acid-induced ...
Craviso Gale L GL Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557-0318, United States. Electronic address: - - 2014
High intensity, nanosecond duration electric pulses (NEPs) permeabilize plasma membranes causing osmotic cell swelling that can elicit a wide variety of cellular effects. This study examined the possibility that cell swelling is the mechanism by which 5ns NEPs trigger the release of catecholamines from neuroendocrine adrenal chromaffin cells. Swelling was ...
Granier Celine J CJ Child Health Institute of New Jersey, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, NJ 08901, USA rabsonab@rwjms.rutgers.edu - - 2014
PDCD2 (programmed cell death domain 2) is a highly conserved, zinc finger MYND domain-containing protein essential for normal development in the fly, zebrafish and mouse. The molecular functions and cellular activities of PDCD2 remain unclear. In order to better understand the functions of PDCD2 in mammalian development, we have examined ...
Yang Nae-Cherng NC School of Nutrition, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung City, 40201, Taiwan, ROC, - - 2014
Calorie restriction (CR) extends lifespan in a remarkable range of organisms. However, the mechanisms of CR related to the longevity effects are not fully elucidated to date. Using human fibroblast Hs68 (Hs68) cells cultured at a lower level of medium glucose (i.e., glucose restriction; GR) to mimic CR, we investigated ...
Bi Fang-Cheng FC State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R. - - 2014
Arabidopsis thaliana plants that lack ceramide kinase, encoded by ACCELERATED CELL DEATH5 (ACD5), display spontaneous programmed cell death late in development and accumulate substrates of ACD5. Here, we compared ceramide accumulation kinetics, defense responses, ultrastructural features, and sites of reactive oxygen species (ROS) production in wild-type and acd5 plants during ...
Reuther C C Institut für Pharmakologie und Klinische Pharmazie, Fachbereich Pharmazie, Philipps-Universität Marburg, Karl-von-Frisch-Straße 1, 35032, Marburg, Germany, - - 2014
It is well-established that activation of proteases, such as caspases, calpains and cathepsins are essential components in signaling pathways of programmed cell death (PCD). Although these proteases have also been linked to mechanisms of neuronal cell death, they are dispensable in paradigms of intrinsic death pathways, e.g. induced by oxidative ...
Mihaly S R SR Department of Biological Sciences, North Carolina State University, Environmental and Molecular Toxicology, Raleigh, NC 27695-7633, - - 2014
Programmed cell death, a physiologic process for removing cells, is critically important in normal development and for elimination of damaged cells. Conversely, unattended cell death contributes to a variety of human disease pathogenesis. Thus, precise understanding of molecular mechanisms underlying control of cell death is important and relevant to public ...
Hu Wentao W Department of Space Radiobiology; Key Laboratory of Heavy Ion Radiation Biology and Medicine; Institute of Modern Physics; Chinese Academy of Sciences; Lanzhou, China; University of Chinese Academy of Sciences; Beijing, PR - - 2014
The mechanisms of radiation-induced bystander effects (RIBE) have been investigated intensively over the past two decades. Although quite a few reports demonstrated that cytokines such as TGF-β1 are induced within the directly irradiated cells and play critical roles in mediating the bystander effects, little is known about the signaling pathways ...
Rastetter Raphael H RH Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, - - 2014
The two main functions of the ovary are the production of oocytes, which allows the continuation of the species, and secretion of female sex hormones, which control many aspects of female development and physiology. Normal development of the ovaries during embryogenesis is critical for their function and the health of ...
Minina Elena A EA Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Box 7080, 75007 Uppsala, - - 2014
Autophagy plays multiple, often antagonistic roles in plants. In particular, cytoprotective functions of autophagy are well balanced by cell death functions to compensate for the absence of apoptosis culminating in phagocytic clearance of dead cells. If autophagy is indeed required for plant programmed cell death (PCD), then what place does ...
Jin Zhe Z Department of Joint Surgery and Sports Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. - - 2014
Chondrosarcomas rank as the third most common type of bone tumors. In the present study, we demonstrated that expression of the apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) was higher in cancer tissues when compared to that in normal tissues. In order to further investigate the effects of APOBEC3B expression, we ...
Iwahori Kota K 1] Center for Cell and Gene Therapy, Texas Children's Hospital, Houston Methodist, Baylor College of Medicine, Houston, Texas 77030 [2] Texas Children's Cancer Center, Texas Children's Hospital, Baylor College of Medicine, Houston, Texas - - 2014
Adoptive immunotherapy with antigen-specific T cells has shown promise for the treatment of malignancies. However, infused T cells are unable to redirect resident T cells, limiting potential benefit. While the infusion of bispecific T-cell engagers can redirect resident T cells to tumors, these molecules have a short half-life, and do ...
Yang Yi Y Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, Guangdong, 510080, China; Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, 510080, - - 2014
Multiple nodes in the one-carbon metabolism pathway play important regulatory roles in cancer cell growth and tumorigenesis. The specific biological functions of metabolic enzymes in regulating the signaling pathways that are associated with tumor cell growth and survival, however, remain unclear. Our current study found that phosphoserine aminotransferase 1 (PSAT1), ...
Wilson Thomas C TC Department of Pathology, University of Iowa, 200 Hawkins Drive, Iowa City, IA, 52242, - - 2014
Basal cell adenoma and basal cell adenocarcinoma represent uncommon basaloid salivary gland neoplasms that show marked morphologic similarity. We wished to compare clinical outcome and morphologic features as well as growth and proliferation associated markers for both neoplasms. We reviewed the pathologic features of 70 neoplasms diagnosed as basal cell ...
Uchida Makoto M Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, - - 2014
Corynebacterium glutamicum is known to perform a unique form of cell division called post-fission snapping division. In order to investigate the mechanism of cell division of this bacterium, we isolated temperature-sensitive mutants from C. glutamicum wild-type strain ATCC 31831, and found that one of them, M45, produced high frequencies of ...
Gudarzi Hoda H a Department of Biology , Faculty of Science, Kharazmi University , Tehran , - - 2014
Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of ...
Singh Purva - - 2014
Mesenchymal cell condensation is the initiating event in endochondral bone formation. Cell condensation is followed by differentiation into chondrocytes accompanied by induction of chondrogenic gene expression. Gene mutations involved in chondrogenesis cause chondrodysplasias and other skeletal defects. Using mesenchymal stem cells (MSCs) in an in vitro chondrogenesis assay, we found ...
D'Souza Sonia S The Institute for Complex Engineered Systems, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA 15213, - - 2014
The field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago. One remaining obstacle, especially for conjugation of polymers to cells, has been that exquisite control over polymer structure and functionality has not been used ...
Song Yongxin Y Department of Marine Engineering, Dalian Maritime University, Dalian, 116026, P. R. - - 2014
An electrokinetic microfluidic chip is developed to detect and sort target cells by size from human blood samples. Target Cell detection is achieved by a differential resistive pulse sensor (RPS) based on the size difference between the target cell and the other cells. Once a target cell is detected, the ...
LaLonde Alexandra A Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY, - - 2014
The effects of insulator-based dielectrophoresis (iDEP) manipulation on cell viability were investigated by varying operating conditions and the shape of the insulating structures. Experiments were conducted with E. coli, B. subtilis and S. cerevisiae cells by varying the applied potential (300-1000 V), exposure time (1-4 min) and composition of the ...
Tang Liying L Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei, Beijing 100700, China; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, - - 2014
Six spirostanol glycosides (1-6) and 12 known compounds (7-18) were isolated from the underground parts of Dracaena thalioides (Agavaceae). Their structures were determined by spectroscopic analysis, including 2D NMR spectroscopic data, and chemical transformations. The isolated compounds were evaluated for cytotoxic activity against HL-60 human leukemia cells. Compounds 1, 3-6, ...
Dehne Tilo T Berlin-Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Berlin, - - 2014
The negligible self-repair potential of the myocardium has led to cell-based tissue engineering approaches to restore heart function. There is more and more consensus that, in addition to cell development, paracrine effects in particular play a pivotal role in the repair of heart tissue. Here, we present two complementary murine ...
Xiang Fenfen F Department of Laboratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. - - 2014
The purpose of the present study was to investigate the relationship between myeloid differentiation primary response gene 88 (MyD88) expression and the resistance to paclitaxel of A549 lung cancer cells. In order to achieve MyD88 gene overexpression or knockdown in the A549 cell line, the cells were infected with lentivirus carrying ...
Piekarski A L AL Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville - - 2014
The major objectives of this study were to compare cell bioenergetics in 2 avian liver cell lines under control conditions and in response to oxidative stress imposed by 4-hydroxy 2-nonenal (4-HNE). Cells in this study were from a chemically immortalized Leghorn male hepatoma (LMH) cell line and a spontaneously immortalized ...
Allaman-Pillet Nathalie N IRO, Institute for Research in Ophthalmology - - 2014
Retinoblastoma is the most common pediatric intraocular neoplasm. While retinoblastoma development requires the inactivation of both alleles of the RB tumor-suppressor gene (RB1) in the developing retina, additional genomic changes are involved in tumor progression, which progressively lead to resistance of tumor cells to death. Therapeutics acting at very downstream ...
Panopoulos Andreas A *Tumor Initiation and Maintenance Program, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037 - - 2014
Tetraploidy can arise from various mitotic or cleavage defects in mammalian cells, and inheritance of multiple centrosomes induces aneuploidy when tetraploid cells continue to cycle. Arrest of the tetraploid cell cycle is therefore potentially a critical cellular control. We report here that primary rat embryo fibroblasts (REF52) and human foreskin ...
Lechuga Susana S Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA 23298, - - 2014
Trans-differentiation of epithelial cells into mesenchymal cells and myofibroblasts plays an important role in tumor progression and tissue fibrosis. Such epithelial plasticity is accompanied by dramatic reorganizations of the actin cytoskeleton, although mechanisms underlying cytoskeletal effects on epithelial trans-differentiation remain poorly understood. In the present study, we observed that selective ...
Osborne Lukas D LD *Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC, - - 2014
Recent studies implicate a role for cell mechanics in cancer progression. The epithelial to mesenchymal transition (EMT) regulates the detachment of cancer cells from the epithelium and facilitates their invasion into stromal tissue. While classic EMT hallmarks include loss of cell-cell adhesions, morphology changes, and increased invasion capacity, little is ...
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