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Results 301 - 350 of 2028
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Ringer Lymor L Department of Oncology and Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA. Contributed equally to this - - 2014
The p53 tumor suppressor protein plays a crucial role in influencing cell fate decisions in response to cellular stress. As p53 elicits cell cycle arrest, senescence or apoptosis, the integrity of the p53 pathway is considered a key determinant of anti-tumor responses. p53 can also promote autophagy, however the role ...
Chen Dongdong - - 2014
Glioblastoma multiforme (GBM) is very difficult to treat with conventional anti-cancer/anti-apoptotic drugs. We tested the hypothesis that inhibition of Na+/K+-ATPase causess a mixed or hybrid form of concurrent apoptosis and necrosis and therefore should enhance anti-cancer effects of chemotherapy on glioblastoma cells. In human LN229 and drug-resistant T98G glioblastoma cell ...
Wang Chunlei - - 2014
BackgroundAmong glioma treatment strategies, arsenic trioxide (As2O3) has shown efficacy as a therapeutic agent against human gliomas. However, the exact antitumor mechanism of action of As2O3 is still unclear. Mitochondria are considered to be the major source of intracellular reactive oxygen species (ROS), which are known to be associated with ...
Choi Hyong Woo HW Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of - - 2014
Pepper (Capsicum annuum L.) provides a good experimental system for studying the molecular and functional genomics underlying the ability of plants to defend themselves against microbial pathogens. Cell death is a genetically programmed response that requires specific host cellular factors. Hypersensitive response (HR) is defined as rapid cell death in ...
Camley Brian A BA Departments of Physics and Center for Theoretical Biological Physics, University of California, San Diego, La Jolla, CA 92093; Departments - - 2014
Pairs of endothelial cells on adhesive micropatterns rotate persistently, but pairs of fibroblasts do not; coherent rotation is present in normal mammary acini and kidney cells but absent in cancerous cells. Why? To answer this question, we develop a computational model of pairs of mammalian cells on adhesive micropatterns using ...
Vashi Aditya V AV CSIRO Manufacturing Flagship, Clayton, 3169, - - 2014
The present study has evaluated a commercial pericardial material for its capacity to assist as a natural extracellular matrix patch for the delivery and retention of mesenchymal stem cells for cardiac repair. The repair of cardiac tissue with cells delivered by an appropriate bioscaffold is expected to offer a superior, ...
Zhou Jianya J Department of Respiratory Disease, Thoracic Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University,, Hangzhou, - - 2014
To evaluate the clinical value of cell block from malignant pleural effusion (MPE) as an alternative sample versus tumor tissue for anaplastic lymphoma kinase (ALK) detection in patients with advanced non small cell lung cancer (NSCLC). Fifty-two matched samples were eligible for analysis. ALK status was detected by Ventana IHC ...
Maggioni Daniele D a Department of Surgery and Translational Medicine , University of Milan-Bicocca , Monza , - - 2014
In this study the potential anticancer effect of 2 flavonoids, myiricetin (MYR) and naringenin (NAR) has been evaluated on an oral squamous cell carcinoma (OSCC) cell line, SCC-25, and HaCaT cells. Both the flavonoids inhibited SCC-25 cell growth, although NAR selectively affected cancer cells without impairing HaCaT cell growth. The ...
Li Min M Department of Ophthalmology, Shanghai Tenth People's Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai, 200072, P.R. - - 2014
The role of microRNA (miRNA) in proliferative vitreoretinopathy (PVR) progression has not been studied extensively, especially in retinal pigment epithelial-mesenchymal transition (EMT) which is the main reason for formation of PVR. In this study, we first investigated the miRNA expression profile in transforming growth factor beta 1 (TGF-β1) mediated EMT ...
Ma Ji J Central Laboratory, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. - - 2014
microRNAs (miRNAs), as gene expression regulators, have been identified to be closely associated with tumorigenesis. Thus a loss-of-function study is more likely to reveal the biological roles of endogenous miRNAs. Genetic knockout, antisense oligonucleotide inhibitors, and miRNA sponge (miR‑SP) are usually performed to inhibit the activities of miRNAs of interest. ...
Semmler Judith J Institute of Neurophysiology, University of Cologne, Cologne, - - 2014
Background/Aims: In vitro reprogramming of somatic cells holds great potential to serve as an autologous source of cells for tissue repair. However, major difficulties in achieving this potential include obtaining homogeneous and stable cells for transplantation. High electrical activity of cells such as cardiomyocytes (CMs) is crucial for both, safety ...
Cheng Xinlai - - 2014
Cancer cells in the advanced stage show aberrant antioxidant capacity to detoxify excessive ROS resulting in the compensation for intrinsic oxidative stress and therapeutic resistance. PDAC is one of the most lethal cancers and often associated with a high accumulation of ROS. Recent studies identified gold(I) NHC complexes as potent ...
Hill Jacqueline E - - 2014
We have examined a series of selenorhodamine derivatives with amide and thioamide functionality (N,N-diethyl and N-piperidyl) at the 5-position of a 9-(2-thienyl) substituent on the selenorhodamine core for their potential as photosensitizers for photodynamic therapy (PDT) in P-glycoprotein (P-gp)-expressing cells. These compounds were examined for their photophysical properties (absorption, fluorescence, ...
Son Jang-Ho JH Department of Oral and Maxillofacial Surgery, Ulsan University Hospital, College of Medicine, Ulsan University, Ulsan, South - - 2014
Osteoblastic differentiation and bone forming capacity are known to be suppressed under hypoxic conditions. Melatonin has been shown to influence cell differentiation. A number of in vitro and in vivo studies have suggested that melatonin also has an anabolic effect on bone, by promoting osteoblastic differentiation. However, the precise mechanisms ...
Maeda Junko J a  Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, 80523; - - 2014
Potentially lethal damage (PLD) repair has been defined as that property conferring the ability of cells to recover from DNA damage depending on the postirradiation environment. Using a novel cyclin dependent kinase 1 inhibitor RO-3306 to arrest cells in the G2 phase of the cell cycle, examined PLD repair in ...
Wei Zewen - - 2014
Microfluidics based continuous cell electroporation is an appealing approach for high-throughput cell transfection, but cell viability of existing methods as such is usually compromised by adverse electrical or hydrodynamic effects. Here we present the validation of a flow-through cell electroporation microchip, in which dielectrophoretic force was employed to sort viable ...
Cai Erica P EP Toronto General Research Institute, University Health Network, 101 College Street, MaRS Centre/TMDT, Room 10-363, Toronto, ON, M5G 1L7, - - 2014
Diabetes mellitus is characterised by beta cell loss and alpha cell expansion. Analogues of glucagon-like peptide-1 (GLP-1) are used therapeutically to antagonise these processes; thus, we hypothesised that the related cell cycle regulators retinoblastoma protein (Rb) and p107 were involved in GLP-1 action. We used small interfering RNA and adenoviruses ...
Dełeńko Konrad K Department of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland, - - 2014
The mechanisms of plant cell dedifferentiation and the acquisition of totipotency are poorly understood. One of the methods to induce the dedifferentiation process in plant cells is simple and requires the removal of the cell wall. After cell wall removal in protoplasts, large-scale chromatin decondensation is observed (Tessadori et al. ...
Zhao Jing-Ya JY Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University, Wuhan, 430072, P. R. China. - - 2014
Gold nanoclusters (AuNCs) with ultra small sizes and unique fluorescence properties have shown promising potential for imaging the nuclei of living cells. However, little is known regarding the potential cytotoxicity of AuNCs after they enter the cell nucleus. The aim of this study is to investigate whether and how nucleus-targeting ...
Quattrocolo Giulia G Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois - - 2014
Cajal-Retzius cells orchestrate the development of cortical circuits by secreting the glycoprotein reelin. However, their computational functions are still unknown. In fact, the nature of their postsynaptic targets, major neurotransmitter released, as well as the class of postsynaptic receptors activated by their firing remain unclear. Here, we have addressed these ...
Peluso John J - - 2014
Progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 are expressed in rat granulosa cells and spontaneously immortalized granulosa cells (SIGCs) but their biological roles are not well defined. The present studies demonstrate that depleting either Pgrmc1 or Pgrmc2 in SIGCs increases entry into the cell cycle but does not increase ...
Rao Divya K DK University of Michigan, United - - 2014
This paper introduces a strategy to kill selectively multidrug resistant cells that express the ABCG2 transporter (also called breast cancer resistance protein, BCRP). The approach is based on specific stimulation of ATP hydrolysis by ABCG2 transporters with sub-toxic doses of curcumin combined with stimulation of ATP hydrolysis by the Na+ ...
Takahashi Saori S Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, - - 2014
The recent successful establishment of mouse parthenogenetic haploid embryonic stem cells (phESCs) and androgenetic haploid ESCs (ahESCs) has stimulated genetic research not only in vitro but also in vivo because of the germline competence of these cell lines. However, it is difficult to maintain the haploid status over time without ...
Riesco Marta F - - 2014
The possibility of generating Primordial Germ Cells (PGCs) in vitro from non-committed embryonic cells represents an extremely useful tool in current research. PGC in vitro differentiation has been successfully reported in mammals. However, contrary to fish, PGC specification in mammals is an inductive mechanism. This study is the first to ...
Shrestha Gajendra G Department of Biology, Brigham Young University, Provo, UT, USA; M. L. Bean Life Science Museum, Brigham Young University, Provo, UT, - - 2014
Cancer is the second leading cause of human deaths in the USA. Despite continuous efforts to treat cancer over the past 50 years, human mortality rates have not decreased significantly. Natural products, such as lichens, have been good sources of anticancer drugs. This study reports the cytotoxic activity of crude extracts ...
Aniket Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina, - - 2014
Pro-osteogenic stimulation of bone cells by bioactive ceramic-coated orthopedic implants is influenced by both surface roughness and material chemistry; however, their concomitant impact on osteoblast behavior is not well understood. The aim of this study is to investigate the effects of nano-scale roughness and chemistry of bioactive silica-calcium phosphate nanocomposite ...
Chen Chih-Lung CL Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan; Genomics Research Center, Academic Sinica, Taipei 115, - - 2014
A short G1 phase is a characteristic feature of the cell cycle structure of pluripotent cells, and is reestablished during Yamanaka factor-mediated pluripotent reprogramming. How cell cycle control is adjusted to meet the requirements of pluripotent cell fate commitment during reprogramming is less well understood. Elevated levels of cyclin D1 ...
Cao Liang - - 2014
BackgroundThe omentum is one of the initial sites for peritoneal metastasis because it possesses milky spots that provide a microenvironment for cancer cells to readily migrate and grow into micrometastases. This study investigated the role of the CCL22-CCR4 axis in gastric cancer cells selectively infiltrating into milky spots.MethodsGastric cancer MFC ...
Bar-Nur Ori O 1] Massachusetts General Hospital Cancer Center, Boston, Massachusetts, USA. [2] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [3] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA. [4] Department of Stem Cell and Regenerative Biology, Cambridge, Massachusetts, - - 2014
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) upon overexpression of OCT4, KLF4, SOX2 and c-MYC (OKSM) provides a powerful system to interrogate basic mechanisms of cell fate change. However, iPSC formation with standard methods is typically protracted and inefficient, resulting in heterogeneous cell populations. We show ...
Amcheslavsky Alla A Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, - - 2014
Intestinal stem cells in the adult Drosophila midgut are regulated by growth factors produced from the surrounding niche cells including enterocytes and visceral muscle. The role of the other major cell type, the secretory enteroendocrine cells, in regulating intestinal stem cells remains unclear. We show here that newly eclosed scute ...
Tamari Keisuke K Department of Radiation Oncology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, - - 2014
Osteosarcoma is the most common primary bone malignancy in pediatric and adolescent populations. Recurrence and metastatic potential can be due to a subpopulation of cells with stem cell-like characteristics, such as tumor-initiating cells (TICs), which maintain the capacity to regenerate entire tumors. Targeting the TICs in osteosarcoma is a promising ...
Hu Juan J Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong 518055, China. - - 2014
We develop a simple and sensitive method for multiplex detection of lung cancer cells at the single-molecule level, with a detection limit of 15 cells per mL for A549 cells and 4 cells per mL for H23 cells, without the involvement of any sequence-based amplification. This method holds great potential ...
Burdak-Rothkamm Susanne S Centre for Cancer Research & Cell Biology, Queen's University Belfast, Belfast, UK, - - 2014
Radioprotective agents are of interest for application in radiotherapy for cancer and in public health medicine in the context of accidental radiation exposure. Methylproamine is the lead compound of a class of radioprotectors which act as DNA binding anti-oxidants, enabling the repair of transient radiation-induced oxidative DNA lesions. This study ...
Wu Lei L Department of Neurosurgery, The Second Affiliated Hospital, Nanchang University, 1 Minde Road, Nanchang, 330006, Jiangxi, People's Republic of - - 2014
Glioblastoma multiforme (GBM) is the prevalent and most fatal brain tumor in adults. Invasion and a high rate of recurrence largely contribute to the poor prognosis of GBM. The current standard therapy for GBM includes surgery with maximum feasible resection, radiotherapy, and treatment with chemotherapeutic agent temozolomide. Annexin A5 reportedly ...
Hernández-Coronado C G CG 1Departamento Producción Agrícola y Animal,Universidad Autónoma Metropolitana-Xochimilco,Calzada del Hueso 1100,CP 04960,México - - 2014
The follicle destiny towards ovulation or atresia is multi-factorial in nature and involves outcries, paracrine and endocrine factors that promote cell proliferation and survival (development) or unchain apoptosis as part of the atresia process. In several types of cells, sphingosine-1-phospate (S1P) promotes cellular proliferation and survival, whereas ceramide (CER) triggers ...
Yu Gan G Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and - - 2014
PTENP1 is a pseudogene of the PTEN tumor suppression gene (TSG). The functions of PTENP1 in clear cell renal cell carcinoma (ccRCC) have not yet been studied. We found that PTENP1 is down-regulated in ccRCC tissues and cells due to methylation. PTENP1 and PTEN are direct targets of microRNA miR-21 ...
English Diana P DP Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, - - 2014
Solitomab is a novel, bispecific, single-chain antibody that targets epithelial cell adhesion molecule (EpCAM) on tumor cells and also contains a cluster of differentiation 3 (CD3) (T-cell coreceptor) binding region. The authors evaluated the in vitro activity of solitomab against primary chemotherapy-resistant epithelial ovarian carcinoma cell lines as well as ...
Dou Yunling Y Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. - - 2014
Human pharyngeal squamous cell carcinoma (HNSCC) are highly invasive and proliferative and exhibit a poor five-year survival rate, mainly due to poor understanding of HNSCC pathogenesis mechanisms, preventing efficient treatment. Ubiquitin‑specific peptidase 22 (USP22) is an important component of cell cycle regulation, as it indirectly affects chromatin structure via histone ubiquitination ...
Li Jin J Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. - - 2014
In order to determine whether human parthenogenetic embryonic stem (hpES) cells have the potential to differentiate into functional cells, a modified four‑step protocol was used to induce the hpES cells into islet‑like clusters (ILCs) in vitro. Growth factors activin A, retinoic acid, nicotinamide, Exendin‑4 and betacellulin were added sequentially to the hpES cells at ...
Jiang Hao H Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, - - 2014
1-Methylpyrene (1-MP) is a widespread pollutant that is carcinogenic in animals following metabolic activation. Previous studies have shown that benzylic hydroxylation of 1-MP, catalyzed by multiple CYP isoforms, gives rise to 1-hydroxymethylpyrene (1-HMP), which becomes bioreactive following further metabolism by various sulfotransferase (SULT) isoforms. However, the mutagenic and chromosome damaging ...
Lin Chin-Yin CY Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan, - - 2014
Koelreuteria formosana ethanolic extract (KFEE) is obtained from natural plants that are endemic to Taiwan. In a previous study, it was demonstrated that KFEE inhibited low-density lipoprotein (LDL) and prevented oxidized LDL‑induced apoptosis in endothelial cells. In the present study, KFEE was shown to inhibit the invasion and migration of 786‑O‑SI3 renal cell carcinoma (RCC) cells while not ...
Lee Dong Hoon DH Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of - - 2014
Livin is one of the most important members of the inhibitor protein (IAP) family. It is overexpressed in several types of tumors and may have prognostic significance. The purpose of this study was to investigate Livin expression in human oral squamous cell carcinomas (OSCCs) and to determine whether Livin affects ...
Kaku Yoshiko - - 2014
The newly synthesized naftopidil analogue HUHS1015 reduced viability of MKN28 and MKN45 human gastric cancer cells in a concentration (0.3-100 µM)-dependent manner, with the potential greater than that for naftopidil. In the cell cycle analysis, HUHS1015 significantly increased the proportion at the subG1 phase of cell cycling in MKN28 cells. ...
Jonas Kristina K LOEWE Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, 35043 Marburg, Germany. Electronic address: - - 2014
A recent study shows that prolonged inhibition of bacterial cell division causes a block of DNA replication, which is followed by an irreversible cell cycle arrest. The finding indicates a tight coupling between cell division and DNA replication in bacteria.
Kong Dong D Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD - - 2014
Newly formed centrioles in cycling cells undergo a maturation process that is almost two cell cycles long before they become competent to function as microtubule-organizing centers and basal bodies. As a result, each cell contains three generations of centrioles, only one of which is able to form cilia. It is ...
Daum Gabor G Department of Stem Cell Biology, Centre for Organismal Studies, University of Heidelberg, D-69120 Heidelberg, - - 2014
Cell-cell communication is essential for multicellular development and, consequently, evolution has brought about an array of distinct mechanisms serving this purpose. Consistently, induction and maintenance of stem cell fate by noncell autonomous signals is a feature shared by many organisms and may depend on secreted factors, direct cell-cell contact, matrix ...
Ono Yoshihiro J YJ Y Ono, Osaka Medical College, Obstetrics and Gynecology, Takatsuki, - - 2014
Dienogest, a synthetic progestin, was shown to be effective against endometriosis, although it is still unclear how it affects the ectopic endometrial cells. Decorin was shown to be a powerful endogenous tumor repressor acting in a paracrine fashion to limit tumor growth. Our objectives were to examine the direct effects ...
Rahman Atiqur A Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia 7003, Bangladesh. - - 2014
We examined the anticancer effect of limonin and imperatorin on various human cancer cells by MTT assay, and the results showed that imperatorin inhibited the cell growth of SNU 449 (liver cancer) and HCT-15 (colon cancer) cells in a dose-dependent manner, while limonin had less effect. Exposure of different concentrations ...
Xu Wenchao W Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, P.R. China; The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, P.R. - - 2014
Lung cancer is the leading cause of cancer mortality worldwide. A common interest in lung cancer research is the identification of biomarkers for early diagnosis and accurate prognosis. There is increasing evidence that microRNAs (miRNAs) are involved in lung cancer. To explore new biomarkers of chemical exposure in risk assessment ...
Li Qifei Q Department of Biological Engineering, Utah State University, Logan, UT, - - 2014
Traditional approaches to characterize stem cell differentiation are time-consuming, lengthy and invasive. Here, Raman microspectroscopy (RM) and atomic force microscopy (AFM) - both considered as non-invasive techniques - are applied to detect the biochemical and biophysical properties of trophoblast derived stem-like cells incubated up to 10 days under conditions designed ...
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