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Results 251 - 300 of 1613
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Gerlach Jörg C JC Departments of Surgery and Bioengineering, University of Pittsburgh, - - 2014
The transcription factor CCAAT/enhancer binding protein alpha (C/EBPα) has been shown to play an important role in liver development, cell proliferation and differentiation. It is, however, largely unknown, if C/EBPα regulates cell differentiation and proliferation differently in the diverse cell types of the human liver. We investigated the role of ...
He Yong Y School of pharmacy, Anhui key laboratory of bioactivity of natural products, Anhui Medical University, Hefei 230032, China; The Key Laboratory of Anti-inflammatory and Immune medicine (Anhui Medical University), Ministry of Education, Hefei 230032, P.R. China; Institute for Liver Diseases of Anhui Medical University(ILD-AMU). Anhui Medical University, Hefei 230032, - - 2014
Long noncoding RNAs (lncRNAs) are being increasingly recognized as major players in governing fundamental biological processes through diverse mechanisms. Maternally expressed gene 3 (MEG3) is an imprinted gene located at 14q32 that encodes a lncRNA correlated with several human cancers. Recently, the methylation-dependent downregulation of MEG3 has been described in ...
Xie Xinyou X Clinical Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, Hangzhou 310016, - - 2014
Nicotinamide N-methyltransferase (NNMT), an enzyme involved in the biotransformation and detoxification of many drugs and xenobiotic compounds, has been found to be overexpressed in several malignancies, including colorectal cancer. However, the biological function of NNMT and the related mechanisms in colorectal cancer have not been elucidated. In the present study, ...
Bunse C E CE Institute for Transfusion Medicine, Hannover Medical School, Germany; Integrated Research and Treatment Centre Transplantation - IFB-Tx, Hannover Medical School, - - 2014
Heme oxygenase (HO)-1, the inducible isoform of HO, has immunomodulatory functions and is considered a target for therapeutic interventions. In the present study, we investigated whether modulation of HO-1 might have regulatory effects on in vitro T-cell activation. The study examined whether: (1) HO-1 induction by cobalt-protoporphyrin (CoPP) or inhibition ...
Yoritsune Ken-Ichi KI Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka, 812-8581, - - 2014
The cell surface of Schizosaccharomyces pombe is negatively charged due to the presence of pyruvylated oligosaccharides, which is important for cell-cell recognition. However, the mechanism of pyruvate supply to oligosaccharides is not clearly understood. Here, we analyzed three putative phosphoenolpyruvate (PEP) transporter genes (pet1(+) , pet2(+) and pet3(+) ) in ...
Zhou Lei L Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiao tong University, Xi'an - PR - - 2014
PSF1 is a subunit of the GINS complex which is essential for establishment of DNA replication forks, and the progression of the replisome. Previous studies have shown a close relationship between PSF1 and cell cycle in the proliferation of immature cells as well as tumors. The purpose of this study ...
Farrell Jeffrey A JA Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138; email: - - 2014
Many, if not most, embryos begin development with extremely short cell cycles that exhibit unusually rapid DNA replication and no gap phases. The commitment to the cell cycle in the early embryo appears to preclude many other cellular processes that only emerge as the cell cycle slows just prior to ...
Perica Karlo K Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA; Institute of Cell Engineering, Johns Hopkins School of Medicine, Baltimore, MD, - - 2014
Artificial Antigen Presenting Cells (aAPC) are engineered platforms for T cell activation and expansion, synthesized by coupling T cell activating proteins to the surface of cell lines or biocompatible particles. They can serve both as model systems to study basic aspects of T cell signaling and translationally as novel approaches ...
Coperchini Francesca F Unit of Internal Medicine and Endocrinology, Fondazione Salvatore Maugeri I.R.C.C.S., Laboratory for Endocrine Disruptors and Chair of Endocrinology University of Pavia, Via S. Maugeri 10, 27100, Pavia, - - 2014
Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are widely used perfluorinated chemicals (PFCs). Previous studies detected PFOA and PFOS in human tissues including the thyroid gland. There are no studies on the in vitro effects of PFOA and PFOS on thyroid cells. Our study was aimed at evaluating the effect ...
Antunes-Ricardo Marilena M Centro de Biotecnología-FEMSA., Tecnológico de Monterrey-, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., - - 2014
(OFI) contains health-promoting compounds like flavonoids, being the isorhamnetin glycosides the most abundant. We evaluated the effect of OFI extracts with different isorhamnetin glycosides against two different human colon cancer cells (HT-29 and Caco2). The extracts were obtained by alkaline hydrolysis with NaOH at 40 °C during 15, 30 or 60 min. ...
Schelch Karin K Medical University of Vienna, Department of Medicine I, Vienna, Austria ; - - 2014
Rationale: Malignant pleural mesothelioma is an aggressive malignancy characterized by frequent resistance to chemo- and radiotherapy, poor outcome and limited therapeutic options. Fibroblast growth factors and their receptors are potential targets for cancer therapy but their significance in mesothelioma has remained largely undefined. Objectives: To investigate the anti-mesothelioma potential of ...
Kishimoto Masanobu M Division of Gastroenterology and Hepatology, The Third Department of Internal Medicine, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka, 573-1010, Japan, - - 2014
Quiescent (slow-cycling) and active (rapid-cycling) stem cells are demonstrated in small intestines. We have identified significant expression of Smad2/3, phosphorylated at specific linker threonine residues (pSmad2/3L-Thr), in murine stomach, and suggested these cells are epithelial stem cells. Here, we explore whether pSmad2/3L-Thr could serve as a biomarker for small intestine ...
Hattar Katja K Department of Internal Medicine IV/V, University of Giessen and Marburg Lung Center (UGMLC), Klinikstrasse 33, Giessen, - - 2014
The inflammatory tumor microenvironment plays a crucial role in tumor progression. In lung cancer, both bacterial infections and neutrophilia are associated with a poor prognosis. In this study, we characterized the effect of isolated human neutrophils on proliferation of the non-small cell lung cancer (NSCLC) cell line A549 and analyzed ...
Leigh Noel J NJ School of Dentistry, The University of Utah, Salt Lake City, - - 2014
Freshly isolated salivary cells can be plated on an extracellular matrix, such as growth factor-reduced Matrigel (GFR-MG), to induce the formation of three-dimensional (3D) structures. Cells grown on GFR-MG are able to form round structures with hollow lumina, capable of sustaining amylase expression. In contrast, cells grown on plastic do ...
Huang Z-J ZJ Department of Oncology, The Second Affiliated Hospital of Fujian Medical University, Fujian, - - 2014
Esophageal carcinoma is one of the most prevalent cancers with high rate of mortality worldwide. Intravenous and oral-based chemotherapeutic regimens were employed; however all the current treatment modalities are largely unsuccessful. Therefore the main aim of this work was to demonstrate the antitumor efficacy of docetaxel (DTX)-loaded trimethyl chitosan (TMC-DTX) ...
Lee Jeong Seok JS Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul, 130-701, South - - 2014
Human periodontal ligament-derived stem cells (PDLSCs) demonstrate self-renewal capacity and multilineage differentiation potential. In this study, we investigated the transdifferentiation potential of human PDLSCs into pancreatic islet cells. To form three-dimensional (3D) clusters, PDLSCs were cultured in Matrigel with media containing differentiation-inducing agents. We found that after 6 days in culture, ...
Kuroda Kengo K Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Aoba-ku, Sendai, 981-8555, - - 2014
Nonhuman primates are useful animal models for the study of human diseases. However, the number of established cell lines from nonhuman primates is quite limited compared with the number established from other experimental animals. The establishment of nonhuman primate cell lines would allow drug testing on those cell lines before ...
Suetens Annelies A Radiobiology Unit, Expert Group for Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre, Boeretang 200, 2400 Mol, Belgium Radiation Oncology Department and Center for Molecular Imaging, Radiotherapy and Oncology, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain (UCL), B1.5407 Avenue Hippocrate, No. 54-55, 1200 Bruxelles, - - 2014
Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness (RBE). This high ballistic accuracy of particle ...
Yu Zuhu Z Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. - - 2014
miR‑886‑3p has been discovered to be involved in the oncogenesis, progression and metastasis of several types of human cancer. The aim of the present study was to identify the biological function of miR‑886‑3p in clear cell renal cell carcinoma (ccRCC) and to determine its possible molecular mechanisms. miR‑886‑3p was found to ...
Xu Wei-Guo WG Department of Orthopedics, Shanxian County Dongda Hospital, Shanxian, Heze, Shandong 274300, P.R. - - 2014
MicroRNA-135b (miR‑135b) functions as an oncogene in malignant tumors suggesting that it might be a target for malignant tumor therapy, but its role in osteosarcoma has not been investigated to date. In this study, we found that myocardin expression is specifically attenuated in MG63 osteosarcoma cells. Silencing miR‑135b restores the ...
Henderson Kiersten A KA Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United - - 2014
Replicative aging in yeast is asymmetric - mother cells age but their daughter cells are rejuvenated. Here we identify an asymmetry in pH between mother and daughter cells that underlies aging and rejuvenation. Cytosolic pH increases in aging mother cells, but is more acidic in daughter cells. This is due ...
Weis Victoria G VG Vanderbilt - - 2014
Oxyntic atrophy in the stomach leads to chief cell transdifferentiation into spasmolytic polypeptide expressing metaplasia (SPEM). Investigations of preneoplastic metaplasias in the stomach are limited by the sole reliance on in vivo mouse models, due to the lack of in vitro models for distinct normal mucosal lineages and metaplasias. Utilizing ...
Frisco-Cabanos Heidie L HL Institute for Integrated Cell-Material Sciences (WPI-iCeMS) and Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan); Graduate School of Medicine Kyoto University - - 2014
One of the major problems encountered in cell transplantation is the low level of survival of transplanted cells due to detachment-induced apoptosis, called anoikis. The present study reports on the chemical synthesis and biological evaluation of water-soluble molecules that protect suspended cells from anoikis. The synthetic molecules bind to and ...
George Isabelle I Univ Paris Diderot, Sorbonne Paris Cité, Unit of Functional and Adaptive Biology (BFA) (BFA) UMR 8251 CNRS, F-75205, Paris, France. Electronic address: - - 2014
Inhalation is the most frequent route of unintentional exposure to nanoparticles (NPs). Our aim was to compare different in vitro models of human lung epithelial monolayers for their suitability to assess the translocation of 50 nm fluorescently labelled silica NPs (50 nm-SiO2-FITC-NPs). Human bronchial epithelial cell lines NCI-H292 and Calu-3 ...
Kawanishi Kunio K Department of Medicine, Kidney Center, Tokyo, - - 2014
Mesothelial cells are an integral part of the peritoneum and play an important role in maintaining its structural and functional properties. In recent years a number of studies on mesothelial cells have been performed to evaluate the localization, secretional properties and the ability of regeneration and transdifferentiation of these cells. ...
McDonald Courtney A - - 2014
Preterm newborns often, require mechanical respiratory support that can result in ventilation-induced lung injury (VILI), despite exogenous surfactant treatment. Human amnion epithelial cells (hAECs) reduce lung inflammation and resultant abnormal lung development in preterm animals; co-administration with surfactant is a potential therapeutic strategy. We aimed to determine whether hAECs remain ...
Herce Henry D HD Department of Biology; Technische Universität Darmstadt; Darmstadt, - - 2014
Proliferating Cell Nuclear Antigen (PCNA) is a key protein in DNA replication and repair. The dynamics of replication and repair in live cells is usually studied introducing translational fusions of PCNA. To obviate the need for transfection and bypass the problem of difficult to transfect and/or short lived cells, we ...
Riihilä Pilvi P 1] Department of Dermatology, University of Turku and Turku University Hospital, Turku, Finland [2] MediCity Research Laboratory, University of Turku, Turku, - - 2014
The incidence of cutaneous squamous cell carcinoma (cSCC) is rising worldwide. We have examined the role of complement components in the progression of cSCC. Analysis of cSCC cell lines (n=8) and normal human epidermal keratinocytes (n=11) with whole transcriptome profiling (SOLiD), quantitative RT-PCR, and western blotting revealed marked overexpression of ...
Zou Xiao-Yan XY Department of Dermatology, Hubei Maternity and Child Health Hospital, Wuhan, - - 2014
Glyoxalase I (GLO1) is a methylglyoxal detoxification enzyme being implicated in the progression of multiple malignancies. However, currently, the role of GLO1 in human nonmelanoma skin tumors remains unclear. To explore the expression of GLO1 in cutaneous neoplasms and its role in the pathogenesis of skin cancers, we determined the ...
Sengupta Aritra A School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, - - 2014
Efficient intracellular delivery of molecules is needed to modulate cellular behavior for laboratory and medical applications, but is often limited by trade-offs between achieving high intracellular delivery and maintaining high cell viability. Here, we studied photoacoustic delivery of molecules into cells by exposing DU145 human prostrate carcinoma cells to nanosecond ...
Lv Xiaoyan X Department of Dermatology, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, - - 2014
Increasingly recognized importance has been assumed for microRNA (miRNA) in the regulation of the delicate balance of gene expression. In our study, we aimed to explore the regulation role of miR181c towards Six2 in metanephric mesenchyme (MM) cells. Bioinformatics analysis, luciferase assay and semi-quantitative real-time (RT) PCR, subsequently RT PCR, ...
Mizunoya Wataru W Department of Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, - - 2014
In this study, we examined the effects of several egg white proteins (ovalbumin, ovomucoid, ovotransferrin and lysozyme) on proliferation and myotube growth in C2C12 murine myoblast cells. Cell proliferation was measured using a water-soluble tetrazolium salt (WST-8)-based assay and then validated using Giemsa staining. Significant proliferative activities of C2C12 cells ...
Khasabov Sergey G SG University of - - 2014
The rostral ventromedial medulla (RVM) projects to the medullary and spinal dorsal horns and is a major source of descending modulation of nociceptive transmission. Traditionally, neurons in the RVM are classified functionally as ON, OFF and NEUTRAL cells based on responses to noxious cutaneous stimulation of the tail or hind ...
Zhang Lianghui L University of Illinois at Chicago, College of Medicine, Chicago, - - 2014
"These results show that a nucleus can promote the formation of a differentiated intestine cell and at the same time contain the genetic information necessary for the formation of all other types of differentiated somatic cell in a normal feeding tadpole. It is concluded that the differentiation of a cell ...
Zeppernick Felix F Departments of *Pathology and Gynecology and Obstetrics #Oncology, Johns Hopkins Medical Institutions, Baltimore, MD ¶The Wistar Institute, Philadelphia, PA †Department of Gynecology and Obstetrics, University Hospital Aachen, Aachen, Germany ‡Unit of Virus, Lifestyle and Genes, Danish Cancer Society Research Center ∥Juliane Marie Centre, Rigshospitalet, University of Copenhagen, Copenhagen §Department of Pathology, Hvidovre Hospital, Denmark Gynecologic Clinic, Hvidovre, - - 2014
Serous borderline tumor also known as atypical proliferative serous tumor (APST) is the precursor of ovarian low-grade serous carcinoma (LGSC). In this study, we correlated the morphologic and immunohistochemical phenotypes of 71 APSTs and 18 LGSCs with the mutational status of KRAS and BRAF, the most common molecular genetic changes ...
Prasad Vibhu V Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland Molecular Life Sciences Graduate School, ETH and University of Zurich, - - 2014
Cancer cells are susceptible to oncolytic viruses, albeit variably. Human adenoviruses (HAdVs) are widely used oncolytic agents, engineered to produce progeny within the tumor, and elicit bystander effects. We searched for host factors enhancing bystander effects, and conducted a targeted RNA-interference screen against guanine-nucleotide exchange factors (GEFs) of small GTPases. ...
Hur Yoon-Sun YS Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, - - 2014
Arabidopsis thaliana homeobox 12 (ATHB12), a homeodomain-leucine zipper class I (HD-Zip I) gene, is highly expressed in leaves and stems, and induced by abiotic stresses, but its role in development remains obscure. To understand its function during plant development, we studied the effects of loss and gain of function. Expression ...
Wang Linbo L Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, - - 2014
Fucoxanthin, a natural carotenoid, has been reported to have anti-cancer activity in human colon cancer cells, human prostate cancer cells, human leukemia cells, and human epithelial cervical cancer cells. This study was undertaken to evaluate the molecular mechanisms of fucoxanthin against human bladder cancer T24 cell line. MTT analysis results ...
Khoury Nathalie N Department of Natural Sciences, Lebanese American University, Beirut 1102 2801, - - 2014
Kefir, a fermented milk product, exhibits anti‑tumoral activity in vivo; yet its mechanism of action remains elusive. Recent studies have focused on the mechanism of action of kefir on cancer cells in vitro. The current study aims at examining the effect of kefir on cell survival, proliferation, and motility of colorectal cancer (CRC) cells. Kefir's ...
Villa Del Campo Cristina C Departamento de Desarrollo y Reparación Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares (CNIC), c/ Melchor Fernández Almagro, 3, E-28029 Madrid, - - 2014
Heterogeneous anabolic capacity in cell populations can trigger a phenomenon known as cell competition, through which less active cells are eliminated. Cell competition has been induced experimentally in stem/precursor cell populations in insects and mammals and takes place endogenously in early mouse embryonic cells. Here, we show that cell competition ...
Karthaus Wouter R WR Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT, Utrecht, - - 2014
The prostate gland consists of basal and luminal cells arranged as pseudostratified epithelium. In tissue recombination models, only basal cells reconstitute a complete prostate gland, yet murine lineage-tracing experiments show that luminal cells generate basal cells. It has remained challenging to address the molecular details of these transitions and whether ...
Su Kai K CSIRO Manufacturing Flagship, Bayview Avenue, Clayton, VICTORIA 3169, - - 2014
Pelvic organ prolapse is a major hidden burden affecting almost 1 in 4 women. It is treated by reconstructive surgery often augmented with synthetic mesh. To overcome the growing concerns of using current synthetic meshes coupled with the high risk of re-operation, a tissue engineering strategy has been developed, adopting ...
Lee Phil Jun PJ College of Pharmacy, Ajou University, Suwon 443-749, Republic of - - 2014
Heme oxygenase-1 (HO-1) and ATPase inhibitory factor (ATPIF) 1 is often overexpressed in different types of cancer cells. Chrysin is a naturally-occurring flavonoid with antioxidant potentials, but also known to promote apoptosis. We have synthesized four chrysin derivatives and found compounds 1 and 4 remarkably upregulated the expression of HO-1, ...
Dao Pascal - - 2014
A series of triazinic inhibitors of FAK have been recently showed to exert anti-angiogenic activity against HUVEC cells and anti-cancer efficacy against several cancers cell lines. We report herein that we further explored the heterocyclic core of these inhibitors by a fused imidazole ring with the triazine to provide imidazo[1,2-a][1,3,5]triazines. ...
Sarna Michal M Department of Medical Physics and Biophysics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Mickiewicza 30, Krakow, 30-059, Poland; Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, - - 2014
The relationship between melanin pigmentation and metastatic phenotype of melanoma cells is an intricate issue, which needs to be unambiguously determined to fully understand the process of metastasis of malignant melanoma. Despite significant research efforts undertaken to solve this problem, the outcomes are far from being satisfying. Importantly, none of ...
Chen Liang-Yu LY Gamete Biology - - 2014
Peritubular myoid (PM) cells surround the seminiferous tubule and together with Sertoli cells form the cellular boundary of the spermatogonial stem cell (SSC) niche. However, it remains unclear what role PM cells have in determining the microenvironment in the niche required for maintenance of the ability of SSCs to undergo ...
Ohki Takeshi T Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, - - 2014
We have developed a technique for the endoscopic transplantation of cultured autologous oral mucosal epithelial cell sheets to an esophageal ulcer following endoscopic submucosal dissection. The epithelial cell sheets successfully prevented esophageal stricture after endoscopic submucosal dissection. Key technology: epithelial cell sheets cultured from oral mucosal tissue and attached proteins ...
Castellarnau M M Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, - - 2014
This study presents stochastic particle barcoding (SPB), a method for tracking cell identity across bioanalytical platforms. In this approach, single cells or small collections of cells are co-encapsulated within an enzymatically-degradable hydrogel block along with a random collection of fluorescent beads, whose number, color, and position encode the identity of ...
Morales-Cruz Moraima - - 2014
BackgroundCytochrome c is an essential mediator of apoptosis when it is released from the mitochondria to the cytoplasm. This process normally takes place in response to DNA damage, but in many cancer cells (i.e., cancer stem cells) it is disabled due to various mechanisms. However, it has been demonstrated that ...
Greene Carol Ann CA Department of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag, 92019, Auckland, New - - 2014
We previously reported that neural-crest-derived stromal cells from adult human and rat corneas can differentiate into neuron-like cells when treated with neuronal lineage specifying growth factors. However, it remains unclear whether this level of cell plasticity is unique to the corneal stromal cell population present in the eye. In this ...
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