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Results 201 - 250 of 1470
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Di Stefano Bruno B Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation and Universitat Pompeu Fabra, Dr Aiguader 88, 08003 Barcelona, - - 2014
The induction of cell reprogramming by transcription factors into alternative cell fates opens new avenues for regenerative medicine. Thymic epithelial cells that were obtained from fibroblasts by Foxn1 overexpression support the formation of an ectopic thymus following transplantation.
Palusinska-Szysz Marta M Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033, Lublin, - - 2014
Binding of human apolipoprotein E (apoE) to Legionella pneumophila lipopolysaccharide (LPS) was analysed at the molecular level by Fourier-transform infrared spectroscopy, thereby providing biophysical evidence for apoE-L. pneumophila LPS interaction. Atomic force microscopy imaging of apoE-exposed L. pneumophila cells revealed alterations in the bacterial cell surface topography and nanomechanical properties ...
Rosario C O CO 1] Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada [2] Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, - - 2014
Polo family kinase 4 (Plk4) is required for mitotic progression, and is haploinsufficient for tumor suppression and timely hepatocyte polarization in regenerating liver. At the same time, recent evidence suggests that Plk4 expression may have a role in clinical cancer progression, although the mechanisms are not clear. Here we identify ...
Li Lin L 1] School of Ocean, Shandong University, Weihai 264209, China [2] School of Pharmaceutical Sciences, Shandong University, Ji-nan 250012, - - 2014
Aim:Telekin, isolated from the Chinese herb Carpesium divaricatum, has shown anti-proliferation effects against various cancer cells, including hepatocellular carcinoma cells. In this study, we investigated the anti-proliferation mechanisms of telekin in human hepatocellular carcinoma HepG2 cells in vitro.Methods:HepG2 cells were treated with telekin. Cell viability was evaluated using MTT assay. ...
Lee Ji Young JY School of Life Science and Biotechnology, Kyungpook National - - 2014
To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-E2), 2-MeO-E2-induced cell cycle changes and apoptotic events were compared between human colon carcinoma cell lines HCT116 (p53+/+) and HCT116 (p53-/-). When both cell types were exposed to 2-MeO-E2, a reduction in the cell viability ...
Gómez-Lamarca M Jesús - - 2014
Coordinating exit from the cell cycle with differentiation is critical for proper development and tissue homeostasis. Failure to do so can lead to aberrant organogenesis and tumorigenesis. However, little is known about the developmental signals that regulate the cell cycle exit-to-differentiation switch. Signals downstream of two key developmental pathways, Notch ...
Xiao Ting Ting TT Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen 6708 PB, The - - 2014
Legume root nodules are induced by N-fixing rhizobium bacteria that are hosted in an intracellular manner. These nodules are formed by reprogramming differentiated root cells. The model legume Medicago truncatula forms indeterminate nodules with a meristem at their apex. This organ grows by the activity of the meristem that adds ...
Kasahara Atsuko A Department of Pathology and Immunology, University of Geneva, 1211 Geneva, - - 2014
Most, if not all mitochondrial functions, including adenosine-5'-triphosphate (ATP) production and regulation of apoptosis and Ca(2+) homeostasis, are inextricably linked to mitochondrial morphology and dynamics, a process controlled by a family of GTP-dependent dynamin related 'mitochondria-shaping' proteins. Mitochondrial fusion and fission directly influence mitochondrial metabolism, apoptotic and necrotic cell death, ...
Han Seong-Su SS Division of Pediatric Hematology-Oncology, University of Iowa Carver College of Medicine, Iowa City, IA, USA. Electronic address: - - 2014
Piperlongumine (PL), a pepper plant alkaloid from Piper longum, has anti-inflammatory and anti-cancer properties. PL selectively kills both solid and hematologic cancer cells, but not normal counterparts. Here we evaluated the effect of PL on the proliferation and survival of B-cell acute lymphoblastic leukemia (B-ALL), including glucocorticoid (GC)-resistant B-ALL. Regardless ...
Auernheimer Vera V Department of Physics, Biophysics Group, Friedrich-Alexander-University of Erlangen-Nuremberg, - - 2014
We investigated the effect of the point mutation E29R on vinculin under cell mechanical aspects. MEFvcl KO cells were transfected with intact eGFP-vinculin (rescue) or mutant E29R vinculin. Cellular stiffness and adhesion strength of mutant E29R vinculin were considerably higher compared to rescue and MEFvcl KO cells. 2D traction microscopy ...
Liu Mingli M Neuroscience Institute, Morehouse School of Medicine, 720 Westview Drive Southwest, Atlanta, GA 30310, USA; Department of Microbiology, Biochemistry & Immunology, Morehouse School of Medicine, 720 Westview Drive Southwest, Atlanta, GA 30310, USA. Electronic address: - - 2014
Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor in adults with median survival time of 14.6months. A small fraction of cancer stem cells (CSC) initiate and maintain tumors thus driving glioma tumorigenesis and being responsible for resistance to classical chemo- and radio-therapies. It is desirable to ...
Lee Jong Min JM Department of Biological Sciences, College of Biological Science and Biotechnology, Cancer and Metabolism Institute, Konkuk University, Seoul 143-701, Republic of - - 2014
Methoxylated chalcones exert antitumor activities. In the present study, we characterized the cytotoxicity of methylated chalcone derivatives against human colon cancer cells. We synthesized a group of methoxychalcones and explored the molecular mechanisms underlying inhibition of tumor growth by these materials. A new synthetic methoxychalcone, 2'-hydroxy-2,4,6-trimethoxy-5',6'-naphthochalcone (named HMNC-74), most effectively ...
Gibot Laure L Centre de recherche en organogenese experimentale de l1Universite Laval/LOEX, Division of Regenerative Medicine, CHU de Quebec research center/FRQS, Faculty of Medicine, Universite Laval, Quebec city, - - 2014
Chitosan, a natural macromolecule, is widely used in medical and pharmaceutical fields because of its distinctive properties such as bactericide, fungicide and above all its antitumor effects. Although its antitumor activity against different types of cancer had been previously described, its mechanism of action was not fully understood. Coating of ...
Yerokun Tokunbo T Department of Biology, Spelman College, Atlanta, GA, - - 2014
The proliferation in human prostate carcinomas, PC3 and MDA-PCa-2b, was analyzed for cells treated with LLW-3-6 and celecoxib in the presence and absence of sulfasalazine. LLW-3-6 was more potent than celecoxib at mediating a dose-dependent reduction of viable PC3 cells. Co-treatment with a non-lethal dose of sulfasalazine diminished the potency ...
Jacquot Catherine C Equipe IICIMed EA 1155, Laboratoire CPCM, Faculté de Pharmacie, Université de Nantes, Nantes, France - - 2014
Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product cucurbitacin D (CucD) - ...
Minami Kentaro K Department of Molecular Oncology, Kagoshima University, Kagoshima, Japan Department of Clinical Pharmacy and Pharmacology, Kagoshima University, Kagoshima, - - 2014
A previously established arsenite-resistant cell line, KAS, is also resistant to a variety of anticancer drugs. In order to understand responsible molecules for the multidrug resistance phenotype of KAS cells, we examined the expressions of ATP-binding cassette (ABC) transporters and found that the ABCB6 and ABCC1/ multidrug resistance protein 1 ...
Kim Si Hyoung SH Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University, Chuncheon, Republic of - - 2014
The aim of the present study was to evaluate the effect of radicicol, an inhibitor of heat shock protein (hsp) 90, alone or in combination with hsp70 inhibition on survival of anaplastic thyroid carcinoma (ATC) cells. Antitumor activity of radicicol-alone or in combination with the hsp70 inhibitor VER155008 was investigated ...
Yamauchi Takahiro T Department of Hematology and Oncology, Faculty of Medical Sciences, University of Fukui, Matsuoka, Fukui, Japan - - 2014
Forodesine inhibits purine nucleoside phosphorylase, resulting in an accumulation of intracellular dGTP and consequently cell death. 9-β-D-Arabinofuranosylguanine (ara-G) is an active compound of nelarabine that is intracellularly phosphorylated to a triphosphate form, which inhibits DNA synthesis. Both agents show cytotoxicity toward T-cell malignancies. In the present study, we investigated the ...
Huang Weiyuan W Department of Physiology, Medical College, Jinan University, Guangzhou, - - 2014
We previously demonstrated that the growth of the poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z) was more dependent on the activities of volume-activated chloride channels than that of the normal nasopharyngeal epithelial cells (NP69-SV40T). However, the activities and roles of such volume-activated chloride channels in highly differentiated nasopharyngeal carcinoma cells (CNE-1) ...
Balsam Joshua J Division of Biology, Office of Science and Engineering, FDA, Silver Spring, MD 20993, United States; University of Maryland, College Park, MD 20742, United - - 2014
Detection of rare cells, such as circulating tumor cells, have many clinical applications. To measure rare cells with increased sensitivity and improved data managements, we developed an imaging flow cytometer with a streak imaging mode capability. The new streak mode imaging mode utilizes low speed video to capture moving fluorescently ...
Del Vecchio Catherine A CA Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of - - 2014
Malignant carcinomas that recur following therapy are typically de-differentiated and multidrug resistant (MDR). De-differentiated cancer cells acquire MDR by up-regulating reactive oxygen species (ROS)-scavenging enzymes and drug efflux pumps, but how these genes are up-regulated in response to de-differentiation is not known. Here, we examine this question by using global ...
Getchell Rodman G RG a Aquatic Animal Health Program, Department of Microbiology and Immunology, College of Veterinary Medicine , Cornell University , 930 Campus Road, Ithaca , New York 14853 , - - 2014
Abstract The development and characterization of a new cell line, derived from the ovary of Largemouth Bass Micropterus salmoides, is described. Gonad tissue was collected from Largemouth Bass that were electrofished from Oneida Lake, New York. The tissue was processed and grown in culture flasks at approximately 22°C for more ...
Zhang Xiaojun X a Aquatic Microbiology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences , Auburn University , Auburn , Alabama 36849 , - - 2014
Abstract The survival of Aeromonas hydrophila under low-nutrient conditions was investigated in this study. The behavior of three strains isolated from Common Carp Cyprinus carpio (China) and Channel Catfish Ictalurus punctatus (USA) was compared when cells were starved at different temperatures (4, 15, 25, and 35°C) over a 4-week period. ...
Fujikura Ushio U Institut für Biochemie und Biologie, Universität Potsdam, Potsdam-Golm, - - 2014
Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate here that the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in ...
Libby Eric E Santa Fe Institute, Santa Fe, New Mexico, United States of - - 2014
Organisms have increased in complexity through a series of major evolutionary transitions, in which formerly autonomous entities become parts of a novel higher-level entity. One intriguing feature of the higher-level entity after some major transitions is a division of reproductive labor among its lower-level units in which reproduction is the ...
Sonal Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, - - 2014
The epidermis is a stratified epithelium, which forms a barrier to maintain the internal milieu in metazoans. Being the outermost tissue, growth of the epidermis has to be strictly coordinated with the growth of the embryo. The key parameters that determine tissue growth are cell number and cell size. So ...
Zhang Yong Y Department of Pathology, Medical School of Jinan University, Guangzhou, 510632, People's Republic of - - 2014
Rational: Combination therapy to inhibit cancer stem cells may have important clinical implications. Here, we examine the molecular mechanisms by which epigallocatechin gallate (EGCG), a bioactive polyphenol in green tea, inhibits the stem cell characteristics of glioma stem-like cells (GSLCs) and synergizes with temozolomide (TMZ), a DNA-methylating agent commonly used ...
Tsuruya Makoto M Tohoku University Graduate School of Dentistry, 4-1 Seiryo-Machi, Aoba-ku, Sendai, 980-8575, - - 2014
Under the hypothesis that photo-irradiated proanthocyanidin could accelerate wound healing through reactive oxygen species (ROS) formation, we examined the effect of proanthocyanidin on 3T3-L1 mouse fibroblasts with or without photo-irradiation. As a result, irrespective of presence or absence of photo-irradiation, only 1 min exposure of the cells to proanthocyanidin resulted in ...
Prater Michael D MD Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, - - 2014
Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin-myosin interactions, and on average, 65% ...
Oh Bea Jun BJ Division of Endocrinology and Metabolism, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of - - 2014
Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature ...
Gen-Huan Yang Y Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing - - 2014
Objective To establish a stable cell line overexpression heme oxygenase-1(HO-1) mediated by a modified lentivirus system and identify its function. Methods The HO-1 gene was amplified by polymerase chain reaction and cloned into the modified pLentiLox3.7 expression vectors. The recombinant plasmids were transfected into HEK293T cells and the HO-1 was ...
Vaid Mudit M Department of Dermatology, University of Alabama at Birmingham, Birmingham, - - 2014
Cutaneous malignant melanoma is the leading cause of death from skin diseases and is often associated with activating mutations of the proto-oncogene BRAF. To develop more effective strategies for the prevention or treatment of melanoma, we have examined the inhibitory effects of silymarin, a flavanoid from Silybum marianum, on melanoma ...
Higuchi Masaya M Division of Virology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, - - 2014
Human T-cell leukemia virus type 1 (HTLV-1), an etiological agent of adult T-cell leukemia, immortalizes and transforms primary human T cells in vitro in both an interleukin (IL)-2-dependent and IL-2-independent manner. Expression of the HTLV-1 oncoprotein Tax transforms the growth of the mouse T-cell line CTLL-2 from being IL-2-dependent to ...
Wan Shanshan S Department of Surgery, University of Michigan, Ann Arbor, - - 2014
& Aims: Cancer stem cells (CSCs) can contribute to hepatocellular carcinoma (HCC) progression and recurrence following therapy. The presence of tumor-associated macrophages (TAMs) in patients with HCC is associated with poor outcomes. It is not clear whether TAMs interact with CSCs during HCC development. We investigated whether TAMs affect the ...
Fofaria Neel M NM Department of Biomedical Sciences & Cancer Biology Center, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, - - 2014
Anoikis is an anchorage-independent cell death. Resistance to anoikis is one of the key features of metastatic cells. Here, we analyzed the role of STAT3 in anoikis resistance in melanoma cells leading to metastasis. When grown under anchorage-independent conditions, significant proportion of cells resisted anoikis and these resistant cells had ...
Bisteau Xavier X - - 2014
Comment on: Lubanska D and Porter LA. The atypical cell cycle regulator Spy1 suppresses differentiation of the neuroblastoma stem cell population. Oncoscience. 2014; 1: 336-348.
Shulman Ziv Z Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, - - 2014
T follicular helper (T(FH)) cells select high-affinity, antibody-producing B cells for clonal expansion in germinal centers (GCs), but the nature of their interaction is not well defined. Using intravital imaging, we found that selection is mediated by large but transient contacts between T(FH) and GC B cells presenting the highest ...
Greife Annemarie - - 2014
Notch signalling regulates cell fate in most tissues, promoting precursor cell proliferation in some, but differentiation in others. Accordingly, downregulation or overactivity variously contributes to cancer development. So far, little is known about Notch pathway activity and function in the normal urothelium and in urothelial carcinoma (UC). We have therefore ...
Takahashi Y Y The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Hirakata, - - 2014
The stem cell compartment in the esophageal epithelium is possibly located in the basal layer. We have identified significant expression of Smad2/3, phosphorylated at specific linker threonine residues (pSmad2/3L-Thr), in the epithelial cells of murine stomach and intestine, and have suggested that these cells are epithelial stem cells. In this ...
Reichl Maren R - - 2014
The complexity of biology requires that measurements of biomolecular interactions have to be performed inside the living cell. While electrophoresis is prohibited by the cell membrane inside cells, the movement of molecules along a temperature gradient appears feasible. This thermophoresis could recently quantify binding affinities in vitro at picomolar levels ...
Lin Li-Chiung LC Department of Life Sciences, National Chung Hsing University, Taichung, - - 2014
Signaling pathway(s) responsible for transforming growth factor β (TGFβ)-induced epithelial mesenchymal transition (EMT), invasion and migration of H460 cells (non-small cell lung cancer/NSCLC) was identified in the study. The results showed that TGFβ-induced p(38)/β-catenin/PPARγ signaling pathway played a critical role in the promotion of EMT, invasion and migration of H460 ...
Xiao Li L Department of Pharmacology, The Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-0071, - - 2014
During embryonic development, cell death transforms the solid embryonic cell mass into a hollow structure (cavitation) which allows the surviving cells to differentiate into varied tissues and organs around the cavity. This process can be partly reproduced with embryonic stem cells. However, it is unknown if adult stem cell masses ...
Stoddart Martin J MJ AO Research Institute Davos, 7270 Davos Platz, Switzerland. Electronic address: - - 2014
Regenerative medicine approaches to cartilage tissue repair have mainly been concerned with the implantation of a scaffold material containing monolayer expanded cells into the defect, with the aim to differentiate the cells into chondrocytes. While this may be a valid approach, the secretome of the implanted cells and its effects ...
Penzo-Méndez Alfredo I AI Gastroenterology Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, - - 2014
The study of animal organ size determination has provided evidence of the existence of organ-intrinsic mechanisms that 'sense' and adjust organ growth. Cell competition, a form of cell interaction that equalizes cell population growth, has been proposed to play a role in organ size regulation. Cell competition involves a cell-context ...
Li Jianying J Department of Respiratory Disease, Affiliated Xi'an Central Hospital, Medical school of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710003, China; Center of Cancer Research, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710003, - - 2014
β-Elemene, a novel antitumor plant drug extracted from the traditional Chinese medicinal herb Zedoary, has been shown to be effective against a wide variety of tumors. Recent studies have indicated that β-elemene can inhibit the growth of lung cancer cells; however, the exact mechanism of β-element's action in lung cancer ...
Valsechi Marina Curado - - 2014
Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute ...
Calles Antonio A Lowe Center for Thoracic Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, - - 2014
MET targeted therapies are under clinical evaluation for non-small-cell lung cancer (NSCLC) patients. Tyrosine kinase inhibitors (TKI) against MET have varying degrees of specificity. Tivantinib (ARQ 197) is reported to be a non-ATP competitive selective MET inhibitor. We aimed to compare the activity of tivantinib to established MET TKIs in ...
Sauter D R P DR Section for Cell and Developmental Biology, Department of Biology, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2100, Copenhagen Ø, Denmark, - - 2014
Pancreatic ductal adenocarcinoma (PDAC) has one of the worst survival rates of all cancers. ANO1 (TMEM16A) is a recently identified Ca(2+)-activated Cl(-) channel (CaCC) that is upregulated in several tumors. Although ANO1 was subject to extensive studies in the recent years, its pathophysiological function has only been poorly understood. The ...
Takemoto Kenji K Department of Applied Biological Science, Tokyo University of Science , Noda, Chiba 278-8510, - - 2014
New dihydronaphthoquinone derivatives, karuquinone A (1), karuquinone B (2), and karuquinone C (3), were isolated from a fungal culture broth of Fusarium solani. The structures were determined by interpretation of spectroscopic data (1D/2D NMR, MS, and IR). Three known compounds, javanicin (4), 2,3-dihydro-5-hydroxy-8-methoxy-2,4-dimethylnaphtho[1,2-b]furan-6,9-dione (5), and 5-hydroxydihydrofusarubin C (6), were also ...
Fernandes João Tiago S JT INESC Microsistemas e Nanotecnologias and IN - Institute of Nanoscience and Nanotechnology, R. Alves Redol, 9, 1000-029, Lisbon, - - 2014
Parkinson's disease (PD) is a common age-associated neurodegenerative disorder. The protein α-synuclein (aSyn) is a key factor in PD both due to its association with familial and sporadic cases and because it is the main component of the pathological protein aggregates known as Lewy bodies. However, the precise cellular effects ...
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