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Results 201 - 250 of 3052
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Zhao Shouye S Hepatobiliary Surgery Department, Affiliated Hospital of Jining Medical College, Guhuai Road 79#, Jining, 272029, Shandong, China, - - 2014
To investigate the effects of GelsemiumElegansBenth (GEB) alkaloids extract on tumor growth in vivo and confirm the effects on cell proliferation, cell apoptosis and cell cycle in human liver cancer cell in vitro. Nude mice xenograft model of human liver cancer were constructed to analyze the effects of GEB alkaloids ...
Pandian Sureshbabu Ram Kumar SR Department of Biotechnology, Kalasalingam University, Krishnankoil, 626126, Tamil Nadu, - - 2014
In this study, we demonstrate that L-glutaminase, a marine bacterial enzyme with a molecular weight of 37 kDa, inhibits cancer cell proliferation in vitro through glutamine deprivation. The concentration of the enzyme reducing the viability of HeLa cells to 50% was determined to be 12.5 μg/mL; the function of L-glutaminase in controlling ...
Henderson Ying C YC Departments of Head and Neck Surgery (Y.C.H., Y.C., S.Y.L., G.L.C.), Pathology (M.D.W., A.K.E-N.), Veterinary Medicine and Surgery (M.G.), Molecular and Cellular Oncology (S.Y.L.), and Cancer Biology (G.L.C.), The University of Texas MD Anderson Cancer Center, Houston, TX 77030; Department of Otolaryngology-Head and Neck Surgery (S.H.A), College of Medicine, Seoul National University Bundang Hospital, Kyunggi-do, Korea; Center for Thyroid Cancer (J.R), National Cancer Center, Goyang-si, Gyeonggi-do, Korea; Department of Endocrinology (R.S., B.H.), University of Colorado at Denver Anschutz Medical Campus, Aurora, CO - - 2014
Context: Cell lines are a widely used tool in cancer research. However, despite the relatively high incidence of papillary thyroid carcinoma (PTC), there are only four PTC cell lines available for international research audience. Objective: and Design: The objective of this study was to establish and characterize new PTC cell ...
Nakamura Masataka M Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, - - 2014
Notch signaling is an important regulator for the development and function of both αβ and γδ T cells, whereas roles of Notch signaling in T cell maintenance remain unclear. We reported previously that the Notch-Hes1 pathway was involved in the intrathymic development of naturally occurring IL-17-producing (IL-17(+)) γδ T cells. ...
Wong Chunshu C Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC 20010; Immunology Graduate Program, Program in Biomedical Sciences, University of Michigan, Ann Arbor, MI - - 2014
Wnt signaling is involved in T cell development, activation, and differentiation. However, the role for Wnt signaling in mature naive T cells has not been investigated. In this article, we report that activation of Wnt signaling in T cell lineages by deletion of the Apc (adenomatous polyposis coli) gene causes ...
Gauvin Timothy J TJ Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover NH - - 2014
FMNL3 is a vertebrate-specific formin protein that has previously been shown to play a role in angiogenesis and cell migration. Here, we define the cellular localization of endogenous FMNL3, the dynamics of GFP-tagged FMNL3 during cell migration, and the effects of FMNL3 suppression in mammalian culture cells. The majority of ...
Shirakawa Makoto M Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, - - 2014
Myrosin cells, which accumulate myrosinase to produce toxic compounds when they are ruptured by herbivores, form specifically along leaf veins in Arabidopsis thaliana. However, the mechanism underlying this pattern formation is unknown. Here, we show that myrosin cell development requires the endocytosis-mediated polar localization of the auxin-efflux carrier PIN1 in ...
Rasmussen Izabela I Department of Cellular and Molecular Medicine, Faculty of Health Sciences, Copenhagen University, - - 2014
The entry mechanism of murine amphotropic retrovirus (A-MLV) has not been unambiguously determined. We show here that A-MLV does not internalize by caveolae or other pinocytic mechanism, but by macropinocytosis. Thus A-MLV infection of mouse embryonic fibroblasts deficient for caveolin or dynamin, or NIH-3T3 cells knocked-down for caveolin expression, was ...
Wu Leitao - - 2014
Sertoli cells (SCs) function as 'nurse cells', which play crucial roles in supporting spermatogenesis through establishing a unique and essential environment in the male reproductive tract. Given the important roles of SCs in male fertility, the study was designed to evaluate the effect of Diosgenin, an aglycone of the steroidal ...
Wang Peng P The Second Department of Urology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of - - 2014
Astrocyte elevated gene-1 (AEG-1) is a recently discovered oncogene that has been reported to be highly expressed in various types of malignant tumors, including renal cell carcinoma. However, the precise role of AEG-1 in renal cancer cell proliferation and apoptosis has not been clarified. In this study, we transfected the ...
Adachi Tetsuo T Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 501-1196, Gifu, Japan. Electronic address: - - 2014
Plasma medicine is a rapidly expanding new field of interdisciplinary research that combines physics, chemistry, biology, and medicine. Non-thermal atmospheric pressure plasma can be applied to living cells and tissues and has emerged as a novel technology for cancer therapy. Plasma has recently been shown to affect cells not only ...
Bujok Krystyna K Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fahrstr. 17, 91054 Erlangen, Germany. Electronic address: - - 2014
Prostaglandins such as prostaglandin E2 (PGE2) play a pivotal role in physiological and pathophysiological pathways in gastric mucosa. Little is known about the interrelation of the prostaglandin E (EP) receptors with the prostaglandin transporter OATP2A1 in the gastric mucosa and gastric carcinoma. Therefore, we first investigated the expression of OATP2A1 ...
Habib Christine N CN Department of Pharmacology & Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, - - 2014
The present study was designed to investigate the effect of leptin on estrogen metabolism in prostatic cells. Malignant (PC-3) and benign (BPH-1) human prostate cells were treated with 17-β-hydroxyestradiol (1uM) alone or in combination with leptin (0.4, 4, 40 ng/ml) for 72 h. Cell proliferation assay, immunocytochemical staining of estrogen ...
Li Yongjian Y Department of Oncology, Suzhou Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu 215009, P.R. - - 2014
Diosgenin is a major compound of Dioscoreaceae plants such as yam, which is used as a drug in Traditional Chinese Medicine, and a common vegetable worldwide. The anticancer effect of diosgenin has been reported in various tumor cells, including leukemia, gastric, colorectal, and breast cancer. However, the activity of diosgenin ...
Yang Hai-Bo HB Department of Biological Science, School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan 467044, P.R. - - 2014
The aim of the present study was to investigate the effects of deoxycholic acid (DCA) on BGC‑823 human gastric carcinoma cells and to explore the possible mechanisms underlying any such effects. Cell proliferation was detected using a 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide assay, cell morphology was observed by inverted microscopy, and cell ...
Rahmani Waleed W Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, - - 2014
The dermal papilla (DP) provide instructive signals required to activate epithelial progenitors and initiate hair follicle regeneration. DP cell numbers fluctuate over the hair cycle, and hair loss is associated with gradual depletion/atrophy of DP cells. How DP cell numbers are maintained in healthy follicles remains unclear. We performed in vivo ...
Jagger Daniel J DJ http://orcid.org/0000-0003-2796-5526 Ear Institute, University College London, London WC1X 8EE, United Kingdom - - 2014
The loss of auditory hair cells triggers repair responses within the population of nonsensory supporting cells. When hair cells are irreversibly lost from the mammalian cochlea, supporting cells expand to fill the resulting lesions in the sensory epithelium, an initial repair process that is dependent on gap junctional intercellular communication ...
Liu Yan Y Department of General Surgery, Wujiang No.1 People's Hospital, Suzhou, 215200, - - 2014
Lupeol, a dietary triterpene, present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including anti-cancer activities both in vitro and in vivo. However, the precise mechanism involved remains largely unknown. The present study is conducted to investigate the anti-cancer activity and the underlying mechanisms ...
Khan Rukaiyya Sirajuddin RS a Department of Pharmacy , BITS-Pilani Hyderabad Campus, Jawahar Nagar, Shamirpet Mandal, Hyderabad 500 078 , Telangana , - - 2014
The study was aimed to identify cytotoxic leads from Abutilon indicum leaves for treating glioblastoma. The petroleum ether extract, methanol extract (AIM), chloroform and ethyl acetate sub-fractions (AIM-C and AIM-E, respectively) prepared from AIM were tested for cytotoxicity on U87MG human glioblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. These extracts exhibited ...
Wei Liuping L Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University-Daqing , Daqing , China - - 2014
Abstract Stromal cell-derived factor 1 (CXCL12) and its receptor CXC chemokine receptor 4 (CXCR4) are known to modulate hypoxia-induced pulmonary hypertension (PH) and vascular remodeling by mobilization and recruitment of progenitor cells to the pulmonary vasculature. However, little is known about CXCL12/CXCR4 regulating proliferation and cell cycle progression of pulmonary ...
Fan Qihui Q Department of Mechanical Engineering, University of Hawaii at Manoa, 2540 Dole Street, Holmes Hall 302, Honolulu, USA. - - 2014
Payloads including FITC-Dextran dye and plasmids were delivered into NIH/3T3 fibroblasts using microbubbles produced by microsecond laser pulses to induce pores in the cell membranes. Two different operational modes were used to achieve molecular delivery. Smaller molecules, such as the FITC-Dextran dye, were delivered via a scanning-laser mode. The poration ...
Son Kyung Jin KJ Department of Biomedical Engineering, University of California, Davis, USA. - - 2014
We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could ...
Gayral Marion M INSERM U1037, Toulouse, France, Université Paul Sabatier, Toulouse, France ; - - 2014
As many other cancers, pancreatic ductal adenocarcinoma (PDAC) progression is associated with a series of hallmark changes for cancer cells to secure their own growth success. Yet, these very changes render cancer cells highly sensitive to viral infection. A promising strategy may rely on and exploit viral replication for tumour ...
Sun Tao T Department of Internal Oncology, Liaoning Cancer Hospital and Institute, Shenyang, 110042, China, - - 2014
Ion channel expression and activity may be affected during tumor development and cancer growth. Activation of potassium (K(+)) channels in human breast cancer cells is reported to be involved in cell cycle progression. In this study, we investigated the effects of docetaxel on the delayed rectifier potassium current (I K) ...
Matsushita Yuki Y Department of Clinical Oral Oncology, Unit of Translational Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, - - 2014
Granular cell ameloblastoma is classified as a histological subtype of solid/multicystic ameloblastoma. Usual granular cell ameloblastoma is histologically characterized by granular changes of stellate-like cells located in the inner portion of the epithelial follicles. Here we report a case of another type of granular cell ameloblastoma, showing predominant anastomosing double-stranded ...
Zare Mehrak M Department of Clinical Biochemistry and Laboratory, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , - - 2014
Induced pluripotent stem (iPS) cells are generated from differentiated adult somatic cells by reprogramming them. Unlimited self-renewal, and the potential to differentiate into any cell type, make iPS cells very promising candidates for basic and clinical research. Furthermore, iPS cells can be genetically manipulated for use as therapeutic tools. DNA ...
Yamada Jun J Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, - - 2014
Perineuronal nets (PNNs) are closely associated with parvalbumin-positive (PV+) neurons, and play a major role in controlling developmental neural plasticity. Considering the recent advances in classification of PV+ neurons, here we aimed to clarify whether PNNs might be associated with specific subclasses of PV+ neurons in the hippocampus. In this ...
Wang Yongji Y Shaanxi University of - - 2014
It is well established that the mitochondria of proximal convoluted tubule cells of the kidney are the site of production of circulating 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3]. The production of 1,25(OH)2D3 at this site is tightly regulated. Parathyroid hormone markedly stimulates 1,25(OH)2D3 production, while 1,25(OH)2D3 itself suppresses production. The mechanism of suppression ...
Uen Wu-Ching WC aSchool of Medicine, FuJen Catholic University, New Taipei City bDepartment of Hematology and Oncology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei cNational Institute of Cancer Research, National Health Research Institutes, Miaoli, - - 2014
Metastasis of melanoma cells during the recurrence or the late stage of melanoma has been characterized as the dissemination of tumor cells under anchorage independency. The secreted interleukin-8 (IL-8) and its conical receptors from melanoma cells have been associated with melanoma malignancy. However, their correlations with melanoma cells under anchorage ...
Weinberger Florian F Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck, Martinistr. 52, 20246 Hamburg, Germany. Electronic address: - - 2014
Rationale: Islet-1 positive (Islet-1(+)) cardiac progenitor cells give rise to the right ventricle, atria and outflow tract during murine cardiac development. In the adult heart Islet-1 expression is limited to parasympathetic neurons, few cardiomyocytes, smooth muscle cells, within the proximal aorta and pulmonary artery and sinoatrial node cells. Its role ...
Voxeur Aline A INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, - - 2014
Lignins are cell wall phenolic polymers resulting from monolignol radical coupling. They have characteristically high diversity in their structures which is a direct consequence of the versatile character of the lignification mechanisms discussed in this review. We will relate the latest discoveries regarding the main participants involved in lignin deposition ...
Shang Zhiqun Z Sex Hormone Research Center, Tianjin Institute of Urology, the Second Hospital of Tianjin Medical University, Tianjin, - - 2014
Epithelial-mesenchymal transition (EMT) has been linked to cancer stem-like (CD44+) cell in the prostate cancer (PCa) metastasis. However, the molecular mechanism remains elusive. Here, we found EMT contributed to metastasis in PCa patients failed in androgen deprivation therapy (ADT). Castration TRAMP model also proved PCa treated with ADT promoted EMT ...
Li Henan H School of Chemistry and Chemical Engineering, Southeast University, Jiangning District, Nanjing, 211189, P. R. China. - - 2014
MicroRNA (miRNA) is found to be up-regulated in many kinds of cancer and therefore is classified as an oncomiR. Herein, we design a multifunctional fluorescent nanoprobe (FSiNP-AS/MB) with the AS1411 aptamer and a molecular beacon (MB) co-immobilized on the surface of the fluorescent dye-doped silica nanoparticles (FSiNPs) for target-cell-specific delivery ...
Li Ke K Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. - - 2014
Three new triarylmethane dyes (TAMs), MPCV, DPCV, and AEV, were synthesized and their photodynamic inactivation abilities against E. coli and human pulmonary carcinoma A549 cells were compared to two commercial TAMs, CV and EV. The enhanced hydrophilicity of MPCV and AEV decreases their cellular uptake to A549 cells dramatically. However, ...
Zismanov Victoria V Oncogenetic Laboratory, Meir Medical Center, Kfar Saba, - - 2014
Intensive protein synthesis is a unique and diffe-rential trait of the multiple myeloma (MM) cells. Previously we showed that tetraspanin overexpression in MM cell lines attenuated mTOR and PI3K cascades, induced protein synthesis, activated unfolded protein response (UPR), and caused autophagic death, all suggesting breach of proteosta-sis. Here we assessed the role ...
Scheffler Kathleen K Centre de Recherche, Institut Curie, F-75248 Paris, France; CNRS-UMR144, F-75248 Paris, - - 2014
Cellular morphogenesis relies partly on cell polarization by the cytoskeleton. In the fission yeast Schizosaccharomyces pombe, it is well established that microtubules (MTs) deliver the spatial cue Tea1, a kelch repeat protein, to the tip regions to direct the growth machinery at the cell tips driving the linear extension of ...
Wu C-Y CY Department of Biological Science, National Sun Yat-sen University, Kaohsiung, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung, - - 2014
Reorganization of the actin cytoskeleton is essential for cell motility and chemotaxis. Actin-binding proteins (ABPs) and membrane lipids, especially phosphoinositides PI(4,5)P2 and PI(3,4,5)P3 are involved in the regulation of this reorganization. At least 15 ABPs have been reported to interact with, or regulated by phosphoinositides (PIPs) whose synthesis is regulated ...
Carlson Maia M Division of Biology, Kansas State University, Manhattan KS, - - 2014
Fate mapping with single cell resolution has typically been confined to embryos with completely stereotyped development. The lineages giving rise to the 40 cells of the Ciona notochord are invariant, but the intercalation of those cells into a single-file column is not. Here we use genetic labeling methods to fate ...
Qian Yue Y University of Georgia, Department of Cellular Biology, Athens, GA 30602, - - 2014
Stem cells and their daughters are often associated with and depend on cues from their cellular microenvironment. In Drosophila testes, each Germline Stem Cell (GSC) contacts apical hub cells and is enclosed by cytoplasmic extensions from two Cyst Stem Cells (CySCs). Each GSC daughter becomes enclosed by cytoplasmic extensions from ...
Kojima Taisuke T Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, - - 2014
This paper describes a novel technique to fabricate spatially-defined cell-laden collagen hydrogels, using patterned, non-adhesive polyacrylamide-coated polydimethylsiloxane (PDMS) surfaces as a template. Precisely patterned embedded co-cultures of breast cancer cells and chemokine-producing cells generated with this technique, revealed matrix- and chemokine isoform-dependent migration of cancer cells. CXCL12 chemokine-secreting cells induce ...
Augustine Robin R International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, 686 560, Kerala, - - 2014
In the present study, we have fabricated electrospun poly(ε-caprolactone)-based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision ...
Galván José A - - 2014
The human COL11A1 gene has been shown to be up-regulated in stromal cells of colorectal tumours, but, so far, the immunodetection of procollagen 11A1, the primary protein product of COL11A1, has not been studied in detail in human colon adenocarcinomas. Some cancer-associated stromal cells seem to be derived from bone ...
Umeda Kahoko K Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Honjo 2-2-1, Chuo-ku, Kumamoto 860-0811, - - 2014
Here, we describe a procedure of human iPS cells differentiation into the definitive endoderm, further into albumin-expressing and albumin-secreting hepatocyte, using M15, a mesonephros- derived cell line. Approximately 90 % of human iPS cells differentiated into SOX17-positive definitive endoderm then approximately 50 % of cells became albumin-positive cells, and secreted ALB protein. ...
Reichman Sacha S Institut de la Vision, Institut National de la Santé et de la Recherche Médicale, U968 , 17 Rue Moreau, 75012, Paris, - - 2014
Human induced pluripotent stem (hiPS) cells could be used as an unlimited source of retinal cells for the treatment of retinal degenerative diseases. Although much progress has been made in the differentiation of pluripotent stem cells towards different retinal lineages, the production of retinal cells from hiPS cells for therapeutic ...
Falduto Guido H GH Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, - - 2014
The migratory stage of Trichinella spiralis, the newborn larva (NBL), travels along the pulmonary microvascular system on its way to the skeletal muscle cells. The present work studies the capability of lung cells to kill NBL. For this purpose, in vitro cytotoxicity assays were performed using NBL, lung cell suspensions ...
Pocha Shirin M SM Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California; 93106-9625; email: shirin.pocha@lifesci.ucsb.edu , - - 2014
The process of cell migration is essential throughout life, driving embryonic morphogenesis and ensuring homeostasis in adults. Defects in cell migration are a major cause of human disease, with excessive migration causing autoimmune diseases and cancer metastasis, whereas reduced capacity for migration leads to birth defects and immunodeficiencies. Myriad studies ...
Palmer Richard R Rothamsted Research, Harpenden, AL5 2JQ, - - 2014
Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre. Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain. Wheat and rice grain present near synchronous developmental processes and significantly different endosperm ...
Sun Chunlong C School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210096, China, - - 2014
IR-780, a novel near-infrared (NIR) fluorescent probe, was synthesized and applied to living cells. The probe exhibited good fluorescent characteristic, and cell experiments showed the probe had high affinity and without apparent cytotoxicity. Fluorescent image experiments in living A549 (Human lung adenocarcinoma epithelial cell line) and L929 (mouse fibroblast cell ...
Sowemimo Abimbola A Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria. Electronic address: - - 2014
Enterolobium cyclocarpum (Jacq.) Griseb. is a tropical tree that has folkloric implications against many ailments and diseases including cancer. To explore the ethnopharmacological claims against cancer, the cytotoxicity of the methanolic extract of the leaves, was investigated using the brine shrimp lethality assay, MTT assay using cervical (HeLa) and breast ...
Austin Lauren A LA Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA - - 2014
Nanoparticles (NPs), in particular noble metal nanoparticles, have been incorporated into many therapeutic and biodiagnostic applications. While these particles have many advantageous physical and optical properties, little is known about their intrinsic intracellular effects in biological environments. Here, we report the possible cell death mechanisms triggered in human oral squamous ...
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