Search Results
Results 201 - 250 of 2638
< 1 2 3 4 5 6 7 8 9 10 >
Sui Chengjun C Department of Special Medical Care and Liver Transplantation, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, - - 2014
Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world with poor prognosis. Here, we investigated the role of microRNA 218 (miR-218) in regulating human HCC development. Quantitative PCR (qPCR) was used to compare the expression levels of miR-218 between eight HCC and a normal liver cell lines, ...
Hu Yufeng Y Department of Environmental Sciences & Engineering, College of Resources and Environmental Sciences, China Agricultural University , Beijing, People's Republic of China , - - 2014
Studies on methanotrophs utilizing methane as sole source of carbon and energy are meaningful for governing global warming; although, the isolation of methanotrophs from nature is challenging. Here, surface imprinted polyurethane films were fabricated to selectively capture living methanotrophs from paddy soil. Two tracks of molecularly imprinted film based on ...
Chao Yuewen Y The Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu, - - 2014
Mammalian sterile 20-like 1 (Mst1), an upstream serine/threonine-specific protein kinase of the Hippo pathway, is reported to play important roles in tumor suppression and organ size regulation in mammals via regulating cell proliferation and survival. However, whether it is involved in the pathogenesis of malignant gliomas remains poorly understood. Therefore, ...
Taura Akiko - - 2014
BackgroundHair cells are important for maintaining our sense of hearing and balance. However, they are difficult to regenerate in mammals once they are lost. Clarification of the molecular mechanisms underlying inner ear disorders is also impeded by the anatomical limitation of experimental access to the human inner ear. Therefore, the ...
Chan Michelle T - - 2014
Pancreatic adenocarcinoma is one of the most lethal cancers, yet it remains understudied and poorly understood. Hyperinsulinemia has been reported to be a risk factor of pancreatic cancer, and the rapid rise of hyperinsulinemia associated with obesity and type 2 diabetes foreshadows a rise in cancer incidence. However, the actions ...
Hou Jianwen - - 2014
Detection of dysfunctional and apoptotic cells plays an important role in clinical diagnosis and therapy. To develop a portable and user-friendly platform for dysfunctional and ageing cell detection, we present a facile method to construct 3D patterns on the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) with poly(ethylene glycol) brushes. Normal red ...
Liao Huihua H Department of Urinary Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. - - 2014
V‑myc avian myelocytomatosis viral oncogene homolog (Myc) regulates cell proliferation, differentiation and apoptosis in several types of cancer. N‑myc downstream‑regulated gene 2 (NDRG2) is known to exhibit reduced expression in renal cell carcinoma (RCC) tissues compared with adjacent non‑neoplastic tissues and is an independent poor prognostic factor predicting survival in RCC. ...
Takagi Mutsumi M Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita-ku, Sapporo, 060-8628, Japan, - - 2014
: The cell processing engineering including automatic cell processing and noninvasive cell quality estimation of adherent mammalian cells for regenerative medicine was reviewed. Automatic cell processing necessary for the industrialization of regenerative medicine was introduced. The cell quality such as cell heterogeneity should be noninvasively estimated before transplantation to patient, ...
Balliu Manjola M Department of Experimental and Clinical Medicine, University of Florence, Firenze, - - 2014
Histone deacetylase inhibitors (HDACi) are agents capable of inducing growth arrest and apoptosis in different tumour cell types. Previously, we reported a series of novel HDACi obtained by hybridizing SAHA or oxamflatin with 1,4-benzodiazepines. Some of these hybrids proved effective against haematological and solid cancer cells and, above all, compound ...
Fu X X College of Animal Science and Technology, Northwest A&F University, Shanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Guangzhou 510380, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, - - 2014
In this study, a continuous cell line (named as CPB) was established from Siniperca chuatsi brain and has been subcultured >140 times. CPB cell line predominantly consisted of fibroblast-like cells that could grow better in Leibovitz's L-15 supplemented with 10% foetal bovine serum at 28° C. Polymerase chain reaction amplification ...
Kim Jong Soo - - 2014
Direct reprogramming of somatic cells to pluripotent stem cells entails the obliteration of somatic cell memory and the reestablishment of epigenetic events. Induced pluripotent stem (iPS) cells have been created by reprogramming somatic cells through the transduction of reprogramming factors. During cell reprogramming, female somatic cells must overcome at least ...
Zhang Jie J Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, Hunan, - - 2014
Lactotransferrin (LTF) has been confirmed to act as a tumor suppressor in multiple cancers; however, its roles in oral squamous cell carcinoma (OSCC), one of malignant head and neck carcinomas, has not been explored. Here, the expression of LTF in OSCC tissues and TCA8113 cells was detected with RT-PCR, qPCR, ...
Hao Zhen-Feng ZF Institute of Skin Damage and Repair, General Hospital of Beijing Region of PLA, Beijing, 100700, - - 2014
Inhibition of histone deacetylase (HDAC) activity by HDAC inhibitors (HDACis) results in cancer cell growth inhibition, and HDACis have been revealed as potential anti-skin cancer agents. p21 is a cyclin-dependent kinase inhibitor and an essential regulator of growth inhibition. Recently, we reported that activating transcription factor 3 (ATF3) could significantly ...
Zaldumbide Laura L Department of Pathology, Cruces University Hospital, University of the Basque Country (UPV/EHU), Barakaldo, Bizkaia, - - 2014
Heterogeneity is an inherent event to tumour development that is lately receiving much attention in oncologic research. The topic is being addressed primarily at the molecular level, and results are promising. However, translation to practical medicine is still pending. Our intention in this study is to approach the problem in ...
Huang Jin J State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. - - 2014
Here, we report an intracellular probe termed i-motif-based nano-flares. They could sense pH change in a live cell since they combine cellular transfection, enzymatic protection, and pH detection.
Iriyama Noriyoshi - - 2014
Abstract We investigated the role of ETV6/ARG fusion genes by exposing the HT93A cell line to nilotinib. HT93A cells were cultured with or without nilotinib ± 50 ng/ml of granulocyte colony-stimulating factor (G-CSF). Nilotinib treatment inhibited cell growth by increasing the percentage of cells in the G0/G1 phase through the ...
Johnson-Farley N - - 2014
ABSTRACT Double hit lymphoma (DHL) is recently recognized lymphoma with a survival of less than two years. Both ABT-737, a Bcl-2/Bcl-XL inhibitor and ABT-199, which selectively targets Bcl-2, were potently cytotoxic against DHL cell lines Sc-1 and OcI-LY18, the RL cell line, and primary human DHL cells, but not the ...
Liang Ning N Division of Oncology, Department of Graduate, Weifang Medical College, Jinan, Shandong 261053, P.R. - - 2014
Galectin-3, a β-galactoside-binding lectin, is a cell adhesion molecule involved in the regulation of tumor progression. However, the importance of galectin-3 in Eca-109 human esophageal cancer cells has not yet been elucidated. In the present study, a lentiviral vector was designed for overexpression of galectin-3 in Eca-109 cells following plasmid‑mediated ...
Adusumilli Prasad S PS Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. adusumip@mskcc.org - - 2014
Translating the recent success of chimeric antigen receptor (CAR) T cell therapy for hematological malignancies to solid tumors will necessitate overcoming several obstacles, including inefficient T cell tumor infiltration and insufficient functional persistence. Taking advantage of an orthotopic model that faithfully mimics human pleural malignancy, we evaluated two routes of ...
Sommer Michael G MG Institut für Chemie und Biochemie, Anorganische Chemie, Freie Universität Berlin, Fabeckstraße 34-36, 14195, Berlin - - 2014
Azocarboxamide (azcH) has been combined for the first time with [Ru-Cym] to generate metal complexes with N,N- and N,O-coordination mode, [(Cym)Ru(azc)Cl] and [(Cym)Ru(azcH)Cl](+) [PF6 ](-) . Geometric and electronic structures of the complexes are reported along with their in vitro activities against different tumour cell lines and preliminary results on ...
Obata Yuuki Y Division of Mucosal Barriology, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan Department of Immune Regulation, Graduate School of Medical and Pharmaceutical Sciences Chiba University, Chiba, Japan Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Yokohama, - - 2014
Intrinsic Notch signaling in intestinal epithelial cells restricts secretory cell differentiation. In gut-associated lymphoid tissue (GALT), stromal cells located beneath the follicle-associated epithelium (FAE) abundantly express the Notch ligand delta-like 1 (Dll1). Here, we show that mice lacking Rbpj-a gene encoding a transcription factor implicated in Notch signaling-in intestinal epithelial ...
Wigglesworth Karen - - 2014
Cumulus cells and mural granulosa cells (MGCs) have functionally distinct roles in antral follicles, and comparison of their transcriptomes at a global and systems level can propel future studies on mechanisms underlying their functional diversity. These cells were isolated from small and large antral follicles before and after stimulation of ...
McCauley Heather A HA Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnett Avenue, Cincinnati, OH 45229, - - 2014
The ocular surface epithelia, including the stratified but non-keratinized corneal, limbal and conjunctival epithelium, in concert with the epidermal keratinized eyelid epithelium, function together to maintain eye health and vision. Abnormalities in cellular proliferation or differentiation in any of these surface epithelia are central in the pathogenesis of many ocular ...
Chang Ki-Eun KE Laboratory of Cell Biology, National Cancer Institute, - - 2014
Despite early positive response to platinum-based chemotherapy, the majority of ovarian carcinomas develop resistance and progress to fatal disease. Protein phosphatase 2A (PP2A) is a ubiquitous phosphatase involved in the regulation of DNA damage response and cell cycle checkpoint pathways. Recent studies have shown that LB100, a small molecule inhibitor ...
Zhao Yi Y Microbiology and Immunology Department, Guangdong Medical College, Dongguan, - - 2014
Pokemon has been showed to directly suppress p14(ARF) expression and also to overexpress in multiple cancers. However, p14(ARF)-MDM2-p53 pathway is usually aberrant in colorectal cancer (CRC). The aim is to confirm whether Pokemon plays a role in CRC and explore whether Pokemon works through p14(ARF)-MDM2-p53 pathway in CRC. Immunohistochemistry for ...
Wanasekara Nandula D ND Department of Physics and Astronomy, University of Exeter, Exeter, Devon, UK, EX4 - - 2014
The objective of this study was to develop a predictive model for cell seeding depth in electrospun scaffold as a function of fiber stiffness. Electrospun scaffolds (micron and submicron) and 3T3 fibroblasts are used as scaffold-cell systems under vacuum seeding conditions. Atomic force microscopy is used to determine the Young's ...
Syed Nazia N Institute of Bioinformatics, International Technology Park, Bangalore, 560066, India; Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, 605014, - - 2014
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in Asia. Currently, surgical resection of early stage tumor is the best available treatment. However, most patients present late when surgery is not an option. Data suggests that chemotherapy regimens are inadequate for clinical management of advanced cancer. ...
Yokoo Rayka R Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, - - 2014
Cyanobacteria are a diverse bacterial phylum whose members possess a high degree of ultrastructural organization and unique gene regulatory mechanisms. Unraveling this complexity will require the use of live-cell fluorescence microscopy, but is impeded by the inherent fluorescent background associated with light-harvesting pigments and the need to feed photosynthetic cells ...
Kliková Katarína K Department of Medical Biochemistry, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak - - 2014
Bortezomib (PS-341, or Velcade), reversible inhibitor of 20S proteasome approved for the treatment of multiple myeloma and mantle cell lymphoma, exhibited a cytotoxic effect toward other malignancies including leukaemia. In this study, we have documented that incubation of both HL-60 and K562 leukaemia cells with nanomolar concentrations of bortezomib is ...
Firouzi Fariba F Radiology Technology Department, School of Paramedicine, Shahid Beheshti University of Medical Sciences, Tehran, - - 2014
The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated with ...
Trehan Ashutosh - - 2014
BackgroundG protein-coupled receptors (GPCRs) represent a physiologically and pharmacologically important family of receptors that upon coupling to G¿S stimulate cAMP production catalyzed by adenylyl cyclase. Thus, developing assays to monitor cAMP production is crucial to screen for ligands in studies of GPCR signaling. Primary cell cultures represent a more robust ...
He Zhifeng Z Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, - - 2014
This study was performed to examine whether lipopolysaccharide can influence cell migration and epithelial-mesenchymal transition of oral squamous cell carcinoma. Three oral squamous cell carcinoma cell lines (HSC3, CAL27, and SCC4) were obtained for the study. TLR4 expression in three cell lines was analyzed by Q-PCR and Western blot. After ...
Chen N-J NJ Department of Orthopaedics, Qilu Hospital, Shandong University, Jinan, - - 2014
The aim of the present study was to assess the anticancer activity of capillarisin against human osteosarcoma (HOS) cancer cells in vitro. Cell viability after capillarisin drug treatment and evaluated by MTT assay. The extent of cell death induced by capillarisin was estimated by using lactate dehydrogenase (LDH) assay. The ...
Shen Yang Y Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, P. R. - - 2014
Uptake of nanoparticles (NPs) affects cell migration but the mechanism remains poorly understood. In this study, the amphiphilic block PCL-PEG nano-micelles with well-controlled hydrophilic/hydrophobic chains were used to investigate the effect of internalized nano-micelles on cancer cell migration. Our results indicated that the nano-micelles with medium PCL and PEG chains ...
Yang B B Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing, - - 2014
Background: Over expression of ATP-binding cassette (ABC) transporter protein (ABCG2) in side population cells plays a crucial role in the chemotherapeutic drug efflux. Constitutive expression of redox sensing transcritption factor Nrf2 was shown to be involved in regulation of ABCG2 expression and chemoresistance. In the current study we made an ...
Zhang You Cheng YC Department of General Surgery, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, P.R. - - 2014
Death receptor 3 (DR3) belongs to the tumor necrosis factor (TNF) receptor superfamily, primarily found in lymphoid tissues. Reports have determined that DR3 may also be distributed in numerous types of tumors. Therefore, it is thought that DR3 may have an important role in the process of tumorigenesis. The aim of the present ...
Burckel Hélène H EA 3430, University of Strasbourg, Centre Paul Strauss, Strasbourg, - - 2014
The present in vitro study aimed to assess the effects of combining the mTOR inhibitor RAD001 and temozolomide (TMZ) together with irradiation by either low-linear energy transfer (LET) radiation (γ-rays) or high-LET radiation (fast neutrons) on the growth and cell survival of the human glioblastoma cell line U-87. We observed a strong decrease in ...
Ma Qunfeng Q Department of Thoracic Surgery, Affiliated Hospital of the Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. - - 2014
Based on the three-dimensional modeling structure of human interleukin-24 (hIL-24) and its most likely active position predicted by solvent accessibility and apparent electrostatic properties, a novel hIL-24 peptide M1 was created by computer-guided molecular design. The cytotoxicity and cell selectivity of M1 were examined in three human carcinoma cell lines ...
Zhou Xiaoshuang X Department of Nephrology, Provincial People's Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. - - 2014
The present study investigated the effects of immunosuppressive agents [tacrolimus (Tac), cyclosporine A (CsA), mycophenolic acid (MMF) and methylprednisone (MP)] on the proliferation, cell cycle progression and apoptotic rate of human mesangial cells. Cultured human mesangial cells were treated with several concentrations of the immunosuppressive agents for 24, 48 or ...
Jin Honglei H Zhejiang Provincial Key Laboratory for Technology & Application of Model Organisms, School of Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China. Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, - - 2014
Previous studies on cancer cell invasion were primarily focused on its migration because these two events were often considered biologically equivalent. Here we found that T24T cells exhibited higher invasion but lower migration abilities than T24 cells. Expression of Rho-GDPases was much lower and expression of SOD2 was much higher ...
Silva Jonathan R JR Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri; Department of Biomedical Engineering, Washington University School of Medicine, St. Louis, Missouri; Department of Center for Investigation of Membrane Excitability Diseases, Washington University School of Medicine, St. Louis, - - 2014
In pancreatic ?-cells, K,ATP channels respond to changes in glucose to regulate cell excitability and insulin release. Confirming a high sensitivity of electrical activity to K,ATP activity, mutations that cause gain of K,ATP function cause neonatal diabetes. Our aim was to quantitatively assess the contribution of K,ATP current to the ...
Chen Yi-Fong YF The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, No.91 Hsueh-Shih Road, Taichung, Taiwan; School of Pharmacy, China Medical University, No.91 Hsueh-Shih Road, Taichung, - - 2014
4-Phenylquinolin-2(1H)-one (4-PQ) derivatives can induce cancer cell apoptosis. Additional new 4-PQ analogs were investigated as more effective, less toxic antitumor agents. Forty-five 6,7,8-substituted 4-substituted benzyloxyquinolin-2(1H)-one derivatives were synthesized. Anti-proliferative activities were evaluated using an MTT assay, and structure-activity relationship correlations were established. Compounds 9b, 9c, 9e and 11e were also ...
Zuo Bo - - 2014
Abstract The tyrosine 177 and Src homology 2 domains (SH2) play important roles in linking p185Bcr-Abl to downstream pathways critical for cell growth and survival. However, a mutant p185(Y177FR552L) (p185(YR)), in which the tyrosine 177 and arginine 552 in the SH2 domain were mutated, is still capable of transforming hematopoietic ...
Lu Xiaosheng X Renmin Hospital of Baise, Baise, 533000, - - 2014
Abstract Phytoestrogens are known to prevent tumor progression by inhibiting proliferation and inducing apoptosis in cancer cells. This study aimed to determine the effect of 5,7-dihydroxy-4'-methoxyisoflavone, a phytoestrogen, on proliferation and apoptosis in the human osteosarcoma cell line U2OS. 5,7-dihydroxy-4'-methoxyisoflavone dose-dependently inhibited proliferation in U2OS cells, which was accompanied by ...
Sheppard Donald C DC Departments of Microbiology and Immunology and Medicine, McGill University, Montreal, Quebec H3A 2B4, - - 2014
To infect the host and cause disease, many medically important fungi invade normally nonphagocytic host cells, such as endothelial cells and epithelial cells. Host cell invasion is a two-step process consisting of adherence followed by invasion. There are two general mechanisms of host cell invasion, induced endocytosis and active penetration. ...
Yan Sheng S School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong, NSW, 2522, - - 2014
This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and cells. The device consists of pre-focusing region and sorting region with great potential to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be focused in the pre-focusing region and then sorted in the sorting region. The DEP-active ...
Zhuang Lihong - - 2014
Microelectrode array has been applied for single cell analysis with relative high throughput; however, the cells were typically cultured on the microelectrodes under cell-size microwell traps leading to the difficulty in the functionalization of electrode surface for higher detection sensitivity. Here, nano-ring electrodes embedded under the microwell traps were fabricated ...
Yu Ling L Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China. lingyu12@swu.edu.cn - - 2014
The BRAF(V600E) inhibitor PLX4032 (Vemurafenib) is an FDA-approved new drug for the treatment of metastatic melanomas, which specifically inhibits the RAS/MEK/ERK signaling pathway to control cell proliferation and adhesion. However, no study has been carried out to investigate the role of intracellular oxidative balance in PLX4032-induced tumor growth inhibition. Herein, ...
Lee Mi-Ae MA From the Department of Life Science, Sogang University, Seoul, 121-742, Republic of - - 2014
A pathogenic bacterium, Vibrio vulnificus produces various extracellular proteases including the elastolytic metalloprotease, VvpE. In silico analysis of its genome revealed a VvpEhomologous protease, VvpM whose proteolytic activity was abolished by specific inhibitors against metalloproteases. To investigate whether this newly identified protease has pathogenic role in host interaction in addition ...
Malki Ahmed A Department of Health Sciences, Biomedical Sciences Program, College of Arts and Sciences , Qatar University, Doha , Qatar - - 2014
Abstract In this study, novel thiosemicarbazides and 1,3,4-oxadiazoles were synthesized and evaluated for their anticancer effects on human MCF-7 breast cancer cell lines. Among the synthesized derivatives studied, compound 2-(3-(4-chlorophenyl)-3-hydroxybutanoyl)-N-phenylhydrazinecarbothioamide 4c showed the highest cytotoxicity against MCF-7 breast cancer cells as it reduced cell viability to approximately 15% compared to ...
< 1 2 3 4 5 6 7 8 9 10 >