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Results 201 - 250 of 2050
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Taylor Brandon L BL Departments of Pediatrics and Cellular & Molecular Medicine, Pediatric Diabetes Research Center, University of California - San Diego, La Jolla, CA - - 2014
All forms of diabetes are characterized by a loss of functional beta cell mass, and strategies for expanding beta cell mass could have significant therapeutic benefit. We have recently identified the transcription factor Nkx6.1 as an essential maintenance factor of the functional beta cell state. In addition, Nkx6.1 has been ...
Bellini Márcia Z MZ School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil Integrated Adamantinenses Colleges (FAI), Adamantina, SP, - - 2014
The association between tridimensional scaffolds to cells of interest has provided excellent perspectives for obtaining viable complex tissues in vitro, such as skin, resulting in impressive advances in the field of tissue engineering applied to regenerative therapies. The use of multipotent mesenchymal stromal cells in the treatment of dermo-epidermal wounds is ...
Leng Gareth G Centre for Integrative Physiology, University of Edinburgh, Edinburgh, - - 2014
Here we present a detailed statistical analysis of the discharge characteristics of mitral cells of the main olfactory bulb of urethane-anesthetized rats. Neurons were recorded from the mitral cell layer, and antidromically identified by stimuli applied to the lateral olfactory tract. All mitral cells displayed repeated, prolonged bursts of action ...
Bheda Poonam P Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104/Inserm U964/Université de Strasbourg, 67400 Illkirch, - - 2014
Mechanistically, how epigenetic states are inherited through cellular divisions remains an important open question in the chromatin field and beyond. Defining the heritability of epigenetic states and the underlying chromatin-based mechanisms within a population of cells is complicated due to cell heterogeneity combined with varying levels of stability of these ...
Wu Yuan-Yuan YY Department of Gastroenterology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. - - 2014
The present study aimed to investigate the role of JWA gene in the proliferation, apoptosis, invasion and migration of PANC-1 pancreatic cancer cells and the effect on the MAPK signaling pathway. Human PANC-1 pancreatic cancer cells were cultured in vitro, and small interfering RNA (siRNA) was designed for the JWA ...
- - 2014
Many pesticides are increasingly used in combinations for crop protection. Their chemical stability ensures the presence of such mixtures, both in the workspaces of the operators involved in agricultural activities and in foodstuffs, thus making probable human exposure to such chemicals in the environment. We report an investigation, performed by ...
Liu Jie J Zhanjiang Key Laboratory of Hepatobiliary Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Gunagdong 524001, P.R. - - 2014
Hepatocellular carcinoma (HCC) is a life-threatening disease that is known to exhibit a poor prognosis. Therefore, it is important to identify an effective drug therapy for the treatment of HCC. Dihydromyricetin (DHM) is a flavonoid compound, isolated from the classical Chinese herb Ampelopsis grossedentata, which exhibits multiple pharmacological activities, including ...
Yu Guohua G Department of Pathology, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China ; Department of Pathology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, Shangdong 264000, P.R. - - 2014
Squamous cell carcinoma is a common malignant tumor of the uterine cervix. The present study reports the case of squamous cell carcinoma of the uterine cervix with osteoclast-like giant cells (OGCs) in an 84-year-old female who had suffered from irregular vaginal bleeding for one month. Colposcopy was performed and a ...
Tomizawa Minoru M Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, - - 2014
In the present study, Frizzled-2 (Fz2), a receptor of the Wnt ligand, was investigated as a potential target of molecular therapy for hepatocellular carcinoma (HCC). Quantitative polymerase chain reaction (PCR) was performed to determine the expression levels of Fz2. A surgical specimen of HCC was immunostained with an Fz2 antibody. ...
Hayashi Akinobu A Department of Oncologic Pathology, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, - - 2014
Transforming growth factor β1 (TGFβ1) regulates a variety of cellular functions, including cell growth, apoptosis and differentiation. The aim of the current study was to investigate the alterations of phenotypic events in the long-term exposure of prostate cancer (PCa) cells to TGFβ1 and its effect on macrophage-differentiated cells. The PCa ...
Sun Ying Y Department of Internal Medicine, Shandong Medical College, Jinan, Shandong 250002, P.R. - - 2014
Radiotherapy (RT) is a major modality of hepatoma treatment. However, liver tumors often acquire radioresistance, which contributes to RT failure. The exact mechanisms of the radioresistance in hepatoma cells are largely unknown. Glutathione S-transferase M3 (GSTM3) is a phase II transferase, however, recent studies have suggested that GSTM3 is a ...
Lasalvia Maria M Università degli studi di Foggia, Dipartimento di Medicina Clinica e Sperimentale, Viale Pinto, I-71122 Foggia, - - 2014
Many pesticides are increasingly used in combinations for crop protection. Their chemical stability ensures the presence of such mixtures, both in the workspaces of the operators involved in agricultural activities and in foodstuffs, thus making probable human exposure to such chemicals in the environment. This investigation, performed by means of ...
Miura Hirohito H Department of Oral Physiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima, - - 2014
Taste buds contain ∼60 elongate cells and several basal cells. Elongate cells comprise three functional taste cell types: I, glial cells; II, bitter/sweet/umami receptor cells; and III, sour detectors. Although taste cells are continuously renewed, lineage relationships among cell types are ill-defined. Basal cells have been proposed as taste bud ...
Pletneva Maria A MA From the Departments of Pathology (Drs Pletneva, Andea, Palanisamy, Betz, Wang, Patel, Fullen, and Harms, and Ms Carskadon) and Dermatology (Drs Andea Patel, Fullen, and Harms), and the Michigan Center for Translational Pathology (Drs Palanisamy and Harms, and Ms Carskadon), University of Michigan Medical Center, Ann - - 2014
Clear cell melanoma is a rare clear cell malignancy. Accurate diagnosis of clear cell melanoma requires integration of immunohistochemical and morphologic findings, with molecular studies to rule out clear cell sarcoma. The differential diagnosis includes melanoma, carcinoma, perivascular epithelioid cell tumor, and epidermotropic clear cell sarcoma. We use a case ...
Verhagen I I Department of Animal Sciences, Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands; Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, - - 2014
In higher primates, development of the adult population of Leydig cells has received little attention. Here, the emergence of 3β-hydroxysteroid dehydrogenase (HSD3B) positive cells in the testis of the rhesus monkey was examined during spontaneous puberty, and correlated with S-phase labeling in the interstitium at this critical stage of development. ...
Kurioka A A Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, - - 2014
Mucosal-associated invariant T (MAIT) cells are an innate-like T-cell population restricted by the non-polymorphic, major histocompatibility complex class I-related protein 1, MR1. MAIT cells are activated by a broad range of bacteria through detection of riboflavin metabolites bound by MR1, but their direct cytolytic capacity upon recognition of cognate target ...
Stecklum Maria M Max Delbrück Center for Molecular Medicine, Berlin-Buch, Robert-Rössle-Str. 10, 13125, Berlin, Germany, - - 2014
In the present study, purified human cord blood stem cells were co-cultivated with murine hepatic alpha mouse liver 12 (AML12) cells to compare the effect on endodermal stem cell differentiation by either direct cell-cell interaction or by soluble factors in conditioned hepatic cell medium. With that approach, we want to ...
Liu Shanna S College of Food Science and Bioengineering, Tianjin Agricultural University, 22 Jinjing Road, 300384, Tianjin, People's Republic of - - 2014
Listeria phage endolysin cell wall-binding domain (CBD) from the Listeria phage A500 was fused with flagellar subunit FliC in Escherichia coli, aiming at binding of E. coli cells to Listeria cells, followed by enhanced killing of Listeria by pediocin production. FliC::CBD chimeric flagella were expressed and detected by Western blot. ...
Liu Zanhua Z Department of Neurology, The First Hospital Affiliated to Dalian Medical University, 222 Zhongshan Road, Dalian, 116011, - - 2014
CDKL3 has an important role in regulating cell growth and/or differentiation, and its inactivation is recently reported to be related to non-syndromic mild mental retardation (MR). MR is a common neurological disorder, predominantly characterized by impaired cognitive function. Though genetic factors play a very important role in the pathogenesis of ...
Badisa Ramesh B RB College of Pharmacy and Pharmaceutical Sciences, Florida Agricultural and Mechanical University, Tallahassee, FL, U.S.A. - - 2014
Lung cancer is the second leading cause of mortality among men and women in the U.S. Among different varieties of lung cancer, the non-small cell lung cancer (NSCLC) has the highest frequency comprising about 85% of cases. We evaluated 1-methyl-4-phenylpyridinium ion (MPP(+)) for cytotoxicity against human lung adenocarcinoma A549, human ...
Yamauchi Takahiro T Department of Hematology and Oncology, University of Fukui, Eiheiji, Fukui, Japan - - 2014
Gemtuzumab ozogamicin (GO) consists of the cluster of differentiation 33 (CD33) antibody linked to calicheamicin. The binding of GO to the CD33 antigen on leukemic cells results in internalization and subsequent release of calicheamicin, thereby inducing DNA strand breaks. We hypothesized that the poly (ADP-ribose) polymerase inhibitor olaparib might inhibit ...
Zenkel Matthias M Department of Ophthalmology, University Erlangen-Nürnberg, Erlangen, - - 2014
The hallmark of exfoliation syndrome is the pathologic production and stable accumulation of an abnormal, fibrillar, extracellular material (XFM). Although the exact biochemical composition of the pathologic matrix product is still not known, immunohistochemical and biochemical approaches have shown XFM to represent a highly glycosylated, cross-linked, and enzymatically resistant glycoprotein/proteoglycan ...
Lane Amelia A Institute of Ophthalmology and Advanced Centre for Biochemical Engineering, University College London, London, United Kingdom - - 2014
Human embryonic stem cells (hESCs) are a promising source of retinal pigment epithelium (RPE) cells: cells that can be used for the treatment of common and incurable forms of blindness, such as age-related macular degeneration. Although most hESC lines will produce a number of clusters of pigmented RPE cells within ...
Cheng Aixin A Faculty of Life Sciences and Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, United Kingdom; Institute of Orthopaedics, Chinese People's Liberation Army General Hospital, Beijing, China aixin.cheng@manchester.ac.uk - - 2014
In initial work, we developed a 14-day culture protocol under potential GMP, chemically defined conditions to generate chondroprogenitors from human embryonic stem cells (hESCs). The present study was undertaken to investigate the cartilage repair capacity of these cells. The chondrogenic protocol was optimized and validated with gene expression profiling. The ...
Servin Alain L AL CNRS UMR 8076 BioCIS, Faculty of Pharmacy, Châtenay-Malabry, France, and University Paris-Sud, Faculty of Pharmacy, Châtenay-Malabry, France - - 2014
The pathogenicity and clinical pertinence of diffusely adhering Escherichia coli expressing the Afa/Dr adhesins (Afa/Dr DAEC) in urinary tract infections (UTIs) and pregnancy complications are well established. In contrast, the implication of intestinal Afa/Dr DAEC in diarrhea is still under debate. These strains are age dependently involved in diarrhea in ...
Woo Sang Hyeok SH Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul, Republic of - - 2014
Cyclin A1 is an A-type cyclin that directly binds to CDK2 to regulate cell-cycle progression. In the present study, we found that doxorubicin decreased the expression of cyclin A1 at the protein level in A549 lung cancer cells, while markedly downregulating its mRNA levels. Interestingly, doxorubicin upregulated caspase-1 in a ...
Ghosh Srimoyee S Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125. Electronic address: - - 2014
The Caenorhabditis elegans uterine seam cell (utse) is an H-shaped syncytium that connects the uterus to the body wall. Comprising nine nuclei that move outward in a bidirectional manner, this synctium undergoes remarkable shape change during development. Using cell ablation experiments, we show that three surrounding cell types affect utse ...
Kono Hajime H Department of Internal Medicine, Teikyo University School of Medicine, Tokyo 173-8605, Japan. Electronic address: - - 2014
Cell death cannot go unnoticed. It demands that the surrounding cells clear away the corpses in a manner appropriate to the type of cell death. Dying cells represent a threat to the body that should be eliminated by the host immune response. Inflammasome activation followed by IL-1alpha release and IL-1beta ...
Deep Gagan G Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences ; University of Colorado Cancer Center; University of Colorado Denver, Aurora, - - 2014
Prostate cancer (PCA) is the 2(nd) leading cause of cancer-related deaths among men in the United States. Preventing or inhibiting metastasis-related events through non-toxic agents could be a useful approach for lowering high mortality among PCA patients. We have earlier reported that natural flavonoid silibinin possesses strong anti-metastatic efficacy against ...
Piccolo Stefano S Department of Molecular Medicine, University of Padua School of Medicine, Padua, - - 2014
The transcriptional regulators YAP and TAZ are the focus of intense interest given their remarkable biological properties in development, tissue homeostasis and cancer. YAP and TAZ activity is key for the growth of whole organs, for amplification of tissue-specific progenitor cells during tissue renewal and regeneration, and for cell proliferation. ...
Wu Kaiyin K From the Medizinische Klinik mit Schwerpunkt Nephrologie, Berlin, - - 2014
Whether T-cell-mediated rejection Banff classification type Ib (severe tubulointerstitial rejection) and type IIa (mild vascular rejection) are associated with responses to antirejection therapy and long-term graft survival are unclear. One hundred ten patients were enrolled who had at least 1 episode of T-cell-mediated rejection and whose highest T-cell-mediated rejection severity ...
Lee Jaewon J Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, - - 2014
On page 6706, K. Cho and co-workers develop a facile approach to realize a polymer nanowire-embedded photoactive layer in bulk-heterojunction solar cells. The incorporation of self-assembled nanowires of a donor-acceptor alternating copolymer efficiently facilitates photon harvesting, allowing a significantly enhanced power conversion efficiency of the solar-cell device.
Xiang Tao T Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, - - 2014
To explore the effect of quercetin on the proliferation and apoptosis of HeLa cells, HeLa cells were incubated with quercetin at different concentrations. Cell viability was evaluated by MTT assay, cell apoptosis was detected by Annexin-V/PI double labeled cytometry and DNA ladder assay. Cell cycle was flow cytometrically determined and ...
Gong Tiancheng - - 2014
Modification of single-walled carbon nanotubes (SWNTs) on the surface of monocrystalline silicon solar cells was investigated. The modification was realized by dropping a well-distributed mixture of SWNTs and ethanol with different dosages on the surface of monocrystalline silicon solar cells in the same effective area. The experimental results showed that ...
Muinonen-Martin Andrew J AJ CRUK Beatson Institute, Glasgow, United Kingdom; York Teaching Hospital NHS Foundation Trust, York, United Kingdom; The Leeds Teaching Hospitals NHS Trust, Leeds, United - - 2014
The high mortality of melanoma is caused by rapid spread of cancer cells, which occurs unusually early in tumour evolution. Unlike most solid tumours, thickness rather than cytological markers or differentiation is the best guide to metastatic potential. Multiple stimuli that drive melanoma cell migration have been described, but it ...
Bertuzzi Margherita M Institute for Inflammation and Repair, University of Manchester, Manchester, United - - 2014
Destruction of the pulmonary epithelium is a major feature of lung diseases caused by the mould pathogen Aspergillus fumigatus. Although it is widely postulated that tissue invasion is governed by fungal proteases, A. fumigatus mutants lacking individual or multiple enzymes remain fully invasive, suggesting a concomitant requirement for other pathogenic ...
Zhu Xiao-Jing XJ Institute of Developmental and Regenerative Biology, College of Life and Environmental Science, Hangzhou Normal University, Zhejiang, China; Key Laboratory of Mammalian Organogenesis and Regeneration, Zhejiang, - - 2014
Epidermal stratification of the mammalian skin requires proliferative basal progenitors to generate intermediate cells that separate from the basal layer and are replaced by post-mitotic cells. Although Wnt signaling has been implicated in this developmental process, the mechanism underlying Wnt-mediated regulation of basal progenitors remains elusive. Here we show that ...
Yu Fu-Shun FS Department of Dentist, China Medical University, Taichung, 404, - - 2014
Tetrandrine is a bisbenzylisoquinoline alkaloid that was found in the Radix Stephania tetrandra S Moore. It had been reported to induce cytotoxic effects on many human cancer cells. In this study, we investigated the cytotoxic effects of tetrandrine on human oral cancer HSC-3 cells in vitro. Treatments of HSC-3 cells ...
Kajbafzadeh A-M AM Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences , Tehran , - - 2014
We used a rat model to decellularize and seed alveolar cells on a three-dimensional lung scaffold to preserve alveolar microarchitecture. We verified the preservation of terminal respiratory structure by casting and by scanning electron microscopy (SEM) of the casts after decellularization. Whole lungs were obtained from 12 healthy Sprague-Dawley rats, ...
Vashi Aditya V AV CSIRO Manufacturing Flagship, Clayton, 3169, - - 2014
The present study has evaluated a commercial pericardial material for its capacity to assist as a natural extracellular matrix (ECM) patch for the delivery and retention of mesenchymal stem cells for cardiac repair. The repair of cardiac tissue with cells delivered by an appropriate bioscaffold is expected to offer a ...
Basso F F INSERM U1016, CNRS (UMR 8104), Institut Cochin, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France, Paris, - - 2014
The cyclic AMP/protein kinase A signaling cascade is one of the main pathways involved in the pathogenesis of adrenocortical tumors. The PKA R1A and R2B proteins are the most abundant regulatory subunits in endocrine tissues. Inactivating mutations of PRKAR1A are associated with Carney complex and a subset of sporadic tumors ...
Schwab C L CL Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, - - 2014
Background:Uterine serous carcinomas (USCs) are an aggressive form of uterine cancer that may rely on HER2/neu amplification as a driver of proliferation. The objective of this paper is to assess the sensitivity of USC cell lines with and without HER2/neu gene amplification to afatinib, an irreversible ErbB tyrosine kinase inhibitor, ...
Daud Sulinda S Dental Faculty, Segi University College, Petaling Jaya, - - 2014
The aim of this study was to determine the changes in cell density and morphology of selected cells of the ageing human dental pulp. Changes in cell density and morphology of dental pulp cells over time may affect their capability to respond to tooth injury. One hundred thirty-one extracted teeth ...
Lin Hui - - 2014
G. sulfurreducens PCA cells have been shown to reduce, sorb, and methylate Hg(II) species, but it is unclear whether this organism could oxidize and methylate dissolved elemental Hg(0) as shown for Desulfovibrio desulfuricans ND132. Using Hg(II) and Hg(0) separately as Hg sources in washed cell assays in phosphate buffered saline ...
Harris Leigh K LK Biophysics Program, Stanford University, Stanford, CA, USA; Department of Biochemistry and Howard Hughes Medical Institute, Stanford University, Stanford, CA, - - 2014
Rod-shaped bacteria typically elongate at a uniform width. To investigate the genetic and physiological determinants involved in this process, we studied a mutation in the morphogenetic protein MreB in Caulobacter crescentus that gives rise to cells with a variable-width phenotype, where cells have regions that are both thinner and wider ...
Lesniak A A Bionanotechnology Group, Conway Institute and School of Chemistry, UCD, Belfield, Dublin, Ireland. - - 2014
Multifunctional nanoparticles that actively target specific cells are promising tools for cancer diagnosis and therapy. In this article we review the synthesis and surface chemistry of Fe-Au nanorods and their characterization using microscopy. The diameter of the rods used in this study was selected to be 150-200 nm so that ...
Flachsbarth Kai K Department of Ophthalmology, University Medical Centre Hamburg-Eppendorf, Martinistr. 52, Hamburg, 20246, - - 2014
Purpose: To analyze the neuroprotective effect of intravitreally grafted neural stem (NS) cells genetically modified to secrete ciliary neurotrophic factor (CNTF) on intraorbitally lesioned retinal ganglion cells (RGCs) in adult mice. Methods: Adherently cultivated NS cells were genetically modified to express a secretable variant of mouse CNTF together with the ...
Chen Ke-Hong KH Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. - - 2014
Large tumor suppressor 1 (LATS1) gene is one of the key factors in Hippo signaling pathway. Inactivation of LATS1 by promoter methylation was found in colorectal cancer (CRC), head and neck squamous cell carcinoma (HNSCC), astrocytoma, breast cancer and it was proved to be a tumor suppressor. However, its role is unclear in renal ...
Farag A A Griffith Health Institute, Regenerative Medicine Center, School of Dentistry and Oral Health, Griffith University, Gold Coast, Australia Institute of Health and Biomedical Innovation, Kelvin Grove, Brisbane, - - 2014
The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with ...
Liu Yaming Y Department of Hepatology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. - - 2014
The role of annexin II in hepatitis B virus (HBV)‑associated hepatocellular carcinoma (HCC) remains to be elucidated. Intracellular hepatitis B surface antigen (HBsAg)‑retention contributes to the induction of hepatocarcinogenesis. The present study aimed to investigate the regulation of HBsAg secretion by annexin II expressed in HBV‑producing hepatoma cells. The expression of annexin II was analyzed ...
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