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Results 201 - 250 of 2571
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Malki Ahmed A Department of Health Sciences, Biomedical Sciences Program, College of Arts and Sciences , Qatar University, Doha , Qatar - - 2014
Abstract In this study, novel thiosemicarbazides and 1,3,4-oxadiazoles were synthesized and evaluated for their anticancer effects on human MCF-7 breast cancer cell lines. Among the synthesized derivatives studied, compound 2-(3-(4-chlorophenyl)-3-hydroxybutanoyl)-N-phenylhydrazinecarbothioamide 4c showed the highest cytotoxicity against MCF-7 breast cancer cells as it reduced cell viability to approximately 15% compared to ...
Sato Ryuta R Department of Urology, Oita University Faculty of Medicine, Hasama, Yufu-city, Oita 879-5593, - - 2014
Angiopoietin-like proteins (ANGPTLs), which comprise 7 members (ANGPTL1-ANGPTL7), structurally resemble angiopoietins. We investigated the roles of ANGPTLs in the acquisition of androgen independence and the malignant behavior of human prostate cancer cells. Expression of ANGPTL messenger RNA (mRNA) and proteins were ascertained using RT-qPCR and western blot analysis in human prostate ...
Xiao Yu-Zhou YZ Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. - - 2014
The use of artificial nerves for the repair of peripheral nerve defects is restricted by the limited sources of Schwann cells (SCs). Human mesenchymal stem cell (MSC)‑derived Schwann‑like cells are considered an alternative and desirable cell source. The aim of the present study was to establish a method of inducing ...
Ding Yi Y State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, - - 2014
A polymerizable disulfide paclitaxel (PTX) prodrug was synthesized by the consequential esterification reactions of 3,3'-dithiodipropionic acid (DTPA), a disulfide compound containing two active carboxyl groups, with 2-hydroxyethyl methacrylate (HEMA) and PTX. The structure of the prodrug was confirmed by (1)H NMR characterization. Then, the polymerizable prodrug was copolymerized with poly(ethylene ...
Beke S S Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. Electronic address: - - 2014
The development of bioactive materials is in the premise of tissue engineering. For several years, surface functionalization of scaffolds has been one of the most promising approaches to stimulate cellular activity and finally improve implant success. Herein, we describe the development of a bioactive composite scaffold composed of a biodegradable ...
Wang Xiao Li XL Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, - - 2014
Human umbilical cord mesenchymal stromal cells (hUC-MSCs) hold great potential as a therapeutic candidate to treat diabetes, owing to their unlimited source and ready availability. In this study, we differentiated hUC-MSCs with in vitro-synthesized pancreatic-duodenal homebox 1 (PDX1) messenger (m)RNA into islet-like cell clusters. hUC-MSCs were confirmed by both biomarker ...
Liu Ronghua R Department of Immunology, Shanghai Medical College, Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University, Shanghai 200032, P.R. China ; Biotherapy Research Center of Fudan University, Shanghai 200032, P.R. - - 2014
Paclitaxel (PTX) is the front-line chemotherapeutic agent against human non-small cell lung cancer (NSCLC). However, its therapeutic efficacy is restricted by the increasing frequency of chemotherapeutic resistance in NSCLC. Accumulating evidence has shown the potential role of microRNAs (miRNAs) in the chemotherapeutic sensitivity of cancer cells. Previously it was reported ...
Tomizawa Minoru M Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, - - 2014
Resistance is one limitation of sorafenib in the treatment of hepatocellular carcinoma (HCC). Insulin-like growth factor-1 receptor (IGF-1R) is involved in cancer cell proliferation. To assess the potential synergistic antitumor effects of picropodophyllin (PPP), an IGF-1R inhibitor, HLF and PLC/PRL/5, HCC cells were treated with PPP alone or PPP in ...
Wang Yong Y Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. - - 2014
Long non-coding RNAs (lncRNAs) are novel regulators in cancer biology. BRAF-activated lncRNA (BANCR) is overexpressed in melanoma and has a potential functional role in melanoma cell migration. However, little is known about the role of BANCR in the development of papillary thyroid carcinoma (PTC). In the present study, BANCR expression ...
Wang Ding D Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China ; Key Laboratory of Reproduction and Genetics, Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China ; Experimental Department, Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. - - 2014
The present study aimed to improve the characterization of amniotic fluid cells (AFCs) in order to optimize their use in chromosomal prenatal diagnosis and as seed or stem cells for tissue engineering. The AFCs used in the current study were obtained from three females in their second trimester of pregnancy. ...
DU Xue X Department of Obstetrics and Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. - - 2014
The aim of the present study was to investigate the effect of heat shock protein 90 (Hsp90)-specific inhibitor geldanamycin (GA) on the proliferation and apoptosis induced by vascular endothelial growth factor-C (VEGF-C) in cervical cancer cells. HeLa cells (1×10(6)/ml) in the logarithmic growth phase were incubated without serum for 24 ...
Zuo Yanzhen Y Department of Pharmacology, Chengde Medical University, Chengde, Hebei 067000, P.R. - - 2014
Transforming growth factor-β1 (TGF-β1) is involved in the regulation of trophoblast cell proliferation and invasion. However, the mechanism underlying this process remains unknown, which is predominantly due to the difficulty in obtaining and maintaining primary trophoblast cells in culture over a long period of time. The HTR-8/SVneo cell line is ...
Wang Bing B Department of General Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. - - 2014
Decitabine (DAC), an inhibitor of DNA methyltransferase, demonstrates antitumor activities in various types of cancer. However, its therapeutic potential for cholangiocarcinoma (CCA), one of the most aggressive gastrointestinal malignancies, remains to be explored. The present study investigated the antiproliferative effects of DAC on CCA cells in vitro and in vivo. ...
Dong Hanzhang H Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. - - 2014
The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression ...
Pelton John C JC Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, - - 2014
Blood vessel polarization in the apical-basal axis is important for directed secretion of proteins and lumen formation; yet, when and how polarization occurs in the context of angiogenic sprouting is not well understood. Here, we describe a novel topology for endothelial cells at the tip of angiogenic sprouts in several ...
Liu Chengcheng C St. Jude Children's Research Hospital, Memphis, TN, - - 2014
Diabetes is affecting more than 25.8 million people in the United States, causing huge burden on the health care system and economy. Insulin injection, which is the predominant treatment for diabetes, is incapable of replenishing the lost insulin-producing beta cell in patients. Restoring beta cell mass through replacement therapy such ...
Mineyuki Yoshinobu Y Graduate School of Life Science, University of Hyogo, Shosha 2167, Himeji 671-2280, - - 2014
Precise control of the cell division plane is a prerequisite for plant development. The division site (the position of the division plane insertion) in plant cells is the site along which the cell plate margin joins the parental cell walls. How this division site is determined and established during cell ...
Satir Peter P Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461 - - 2014
This essay records a voyage of discovery from the "cradle of cell biology" to the present, focused on the biology of the oldest known cell organelle, the cilium. In the "romper room" of cilia and microtubule (MT) biology, the sliding MT hypothesis of ciliary motility was born. From the "summer ...
Tanaka Misaki M Dept. of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology 680-4 Kawazu, Iizuka, Fukuoka, - - 2014
Most cells of multicellular organisms have "primary cilia", which are single, non-motile, and sensory cilia. They have been reported to detect mechanical stimulation and transform it into internal cell, but the mechanisms are not still well known. Dermal papilla (DP) cells, which locate in the skin and regulate hair follicle ...
Rouse Michael M Laboratory of Clinical InvestigationLaboratory of Cardiovascular ScienceNational Institute on Aging, Intramural Research Program, National Institutes of Health, 251 Bayview Blvd, Baltimore, Maryland 21224, - - 2014
Resveratrol (RES) and curcumin (CUR) are polyphenols that are found in fruits and turmeric, and possess medicinal properties that are beneficial in various diseases, such as heart disease, cancer, and type 2 diabetes mellitus (T2DM). Results from recent studies have indicated that their therapeutic properties can be attributed to their ...
Lachaier Emma E Laboratoire de Biochimie, Centre de Biologie Humaine (CBH), CHU Amiens Sud, Amiens Cedex, France EA4666, Université de Picardie Jules Verne (UPJV), Amiens Cedex, - - 2014
Ferroptosis is a recently identified form of regulated necrosis that can be experimentally induced in cancer cells with the chemical inducer erastin. Recently, we identified sorafenib, an inhibitor of oncogenic kinases, as an inducer of ferroptosis in hepatocellular carcinoma cells. Whether sorafenib is able to exert its ferroptotic activity in ...
Jarząb Agata A Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, - - 2014
The aim of the present study was to determine the effects of osthole on cell proliferation and viability, cell-cycle progression and induction of apoptosis in human laryngeal cancer RK33 and human medulloblastoma TE671 cell lines. Cell viability was measured by means of the MTT method and cell proliferation by the ...
Sakai Chizuko C Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, - - 2014
The effects of inorganic and organic arsenicals on proliferation, cell-cycle distribution, and apoptosis of all-transretinoic acid (ATRA)-resistant human promyelocytic leukemia HL-60 (HL-60-R2) cells were herein investigated. Cell proliferation was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell-cycle distribution and apoptotic cells were analyzed by flow cytometry. The 50% inhibitory concentrations (IC50 values) ...
Lee Chia-Jen CJ Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan, - - 2014
Aim/Materials and Methods: In order to develop better drugs against non-small cell lung cancer (NSCLC), we screened a variety of compounds and treated the human lung adenocarcinoma cell line A549 with different drug concentrations. We then examined the cell viability using the MTT assay. Data show that a new candidate ...
Nagahara Yukitoshi Y Division of Life Science and Engineering, College of Science and Engineering, Tokyo Denki University, Hatoyama, Hiki-gun, Saitama, Japan - - 2014
Amidoxime derivatives have been previously reported to have potent anti-microbial and anti-tumor activity. Little is known about the tumor cell growth-inhibition mechanism of amidoximes, especially benzamidoxime derivatives. Herein we determined the effects of N-(2-amino-5-chlorobenzoyl)benzamidoxime analogs on mammalian cancer cells. We synthesized four chloride-substituted benzamidoxime analogs from the original benzamidoxime to ...
Rashid Nurshamimi Nor NN Section of Virology, Department of Medicine, Imperial College London, London, U.K. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia - - 2014
It is well-established that HPV E7 proteins, encoded by human papillomavirus (HPV) genes, frequently associated with cervical cancers bind avidly to the retinoblastoma (RB) family of pocket proteins and disrupt their association with members of the E2F transcription factor family. Our previous study showed that the repressive p130-dimerization partner, RB-like, ...
- - 2014
A T-cell transfected with Gag-GFP and CD81-mCherry produced nanotubes contacting three untransfected cells. Gag is present at the tube tip that is connected to the target cell.
Schlegel Natalie C NC Department of Biomedicine, University of Basel, Mattenstrasse 28, 4056, Basel, - - 2014
Epithelial to mesenchymal transition (EMT) is a program defined in epithelial cells and recognized as playing a critical role in cancer progression. Although melanoma is not a cancer of epithelial cells, hallmarks of EMT have been described to play a critical role in melanoma progression. Here we demonstrate that long ...
Zhang Jing - - 2014
BackgroundSOX4 is a transcription factor required for tissue development and differentiation in vertebrates. Overexpression of SOX4 has been reported in many cancers including glioblastoma multiforme (GBM), however, the underlying mechanism of actions has not been studied. In this study, we investigated the role of SOX4 in GBM.MethodsKaplan-Meier analysis was performed ...
Pratt Erica D - - 2014
Limited access to tumor tissue makes repeated sampling and real-time tracking of cancer progression infeasible. Circulating tumor cells (CTCs) provide the capacity for real-time genetic characterization of a disseminating tumor cell population via simple blood draw. However, there is no straightforward method to analyze broadscale genetic rearrangements in this heterogeneous ...
Parry Jean M JM Department of Genetics, University of Pennsylvania Perelman School of Medicine, 415 Curie Boulevard, Philadelphia, PA 19104, USA Department of Biology, Georgian Court University, 900 Lakewood Avenue, Lakewood, NJ 08701, - - 2014
Receptor tyrosine kinase (RTK) signaling through Ras influences many aspects of normal cell behavior, including epithelial-to-mesenchymal transition, and aberrant signaling promotes both tumorigenesis and metastasis. Although many such effects are cell-autonomous, here we show a non-cell-autonomous role for RTK-Ras signaling in the delamination of a neuroblast from an epithelial organ. ...
Francou Alexandre A Aix Marseille Université, CNRS, IBDM UMR 7288, Marseille 13288, - - 2014
Elongation of the vertebrate heart occurs by progressive addition of second heart field (SHF) cardiac progenitor cells from pharyngeal mesoderm to the poles of the heart tube. The importance of these cells in the etiology of congenital heart defects has led to extensive research into the regulation of SHF deployment ...
Tran Cindy S CS Department of Pediatrics, University of California San Francisco, San Francisco, California, United States of - - 2014
Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known about biofilm formation at the epithelial barrier. We have previously shown that ...
Roiko Marijo S MS Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of - - 2014
Pathogenic microbes rely on environmental cues to initiate key events during infection such as differentiation, motility, egress and invasion of cells or tissues. Earlier investigations showed that an acidic environment activates motility of the protozoan parasite T. gondii. Conversely, potassium ions, which are abundant in the intracellular milieu that bathes ...
Yang Chen C 1 Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University , Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China - - 2014
Abstract In this study, the authors evaluate the biological effects of irradiation of hepatocellular carcinoma cells by internal exposure with (125)I-labeled 5-iodo-2'-deoxyuridine ((125)I-UdR)-chitosan drug loading nanoparticles ((125)I-UdR-CS-DLN). The authors observed that accumulation of nanoparticles was significantly (p<0.05) higher in hepatocellular carcinoma cells HepG2 than normal liver cells HL-7702 after treated ...
Lin Xuechi X 1 Department of Medical Laboratory, Changsha Medical University , Changsha, China - - 2014
Abstract Purpose: Taxol is an effective chemotherapeutic agent against epithelial-derived carcinomas, and resistance of carcinoma cells to taxol has developed with the wide prescription of the drug. In this study, five different epithelial carcinoma cell lines were randomly employed to screen the resistant cell line to taxol, and to explore ...
Koch Britta B Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstraße 20, Dresden, D-01069, - - 2014
Biofunctionalized glass microtube structures serve as transparent cell culture scaffolds and are fabricated to impose spatial confinement on single cells on page 1753. S. Sanchez, C. K. Schmidt, and colleagues demonstrate the influence of the resulting cell deformation on the integrity of the nucleus of human osteosarcoma cells and on ...
Pruitt Beth L BL Department of Mechanical Engineering, Stanford University, Stanford, California, United States of America; Cardiovascular Institute, Stanford University, Stanford, California, United States of - - 2014
External forces play complex roles in cell organization, fate, and homeostasis. Changes in these forces, or how cells respond to them, can result in abnormal embryonic development and diseases in adults. How cells sense and respond to these mechanical stimuli requires an understanding of the biophysical principles that underlie changes ...
Lee Yoon-Jin YJ 0000-0002-0911-2485 Soonchunhyung Environmental Health Center for Asbestos, Soonchunhyang University Cheonan Hospital, Cheonan, Korea. ; Division of Molecular Cancer Research, Soonchunhyang Medical Research Institute, Cheonan, - - 2014
Mcl-1 and Bcl-xL, key anti-apoptotic proteins of the Bcl-2 family, have attracted attention as important molecules in the cell survival and drug resistance. In this study, we investigated whether inhibition of Bcl-xL influences cell growth and apoptosis against simultaneous treatment of resveratrol and clofarabine in the human malignant mesothelioma H-2452 ...
Ichwan Solachuddin J A SJ Department of Basic Medical Science, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan 25710, Pahang, Malaysia Human Molecular Cellular Biology Research Unit, Integrated Centre of Research and Animal Care and Use (HMCB-ICRACU), International Islamic University, Kuantan, Pahang, - - 2014
Thymoquinone (TQ) is the main constituent of black seed (Nigella sativa, spp) essential oil which shows promising in vitro and in vivo anti-neoplastic activities in different tumor cell lines. However, to date there are only a few reports regarding the apoptotic effects of TQ on cervical cancer cells. Here, we ...
Shields L L a  Medical Physics Department, St. Luke's Hospital, Highfield Road, Rathgar, Dublin 6, - - 2014
Interest in out-of-field radiation dose has been increasing with the introduction of new techniques, such as volumetric modulated arc therapy (VMAT). These new techniques offer superior conformity of high-dose regions to the target compared to conventional techniques, however more normal tissue is exposed to low-dose radiation with VMAT. There is ...
Zhang Jing J MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, - - 2014
A general view is that Schizosaccharomyces pombe undergoes symmetric cell division with two daughter cells inheriting equal shares of the content from the mother cell. Here we show that CTP synthase, a metabolic enzyme responsible for the de novo synthesis of the nucleotide CTP, can form filamentous cytoophidia in the ...
Lee Lap Man LM Department of Mechanical Engineering, University of Michigan, 2350 Hayward Street, Ann Arbor, MI 48105, USA. - - 2014
Micropipette aspiration measures the mechanical properties of single cells. A traditional micropipette aspiration system requires a bulky infrastructure and has a low throughput and limited potential for automation. We have developed a simple microfluidic device which is able to trap and apply pressure to single cells in designated aspiration arrays. ...
Swaminathan T Raja TR National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, Cochin, 682018, Kerala, India, - - 2014
A new epithelial cell line, Horabagrus brachysoma fin (HBF), was established from the caudal fin tissue of yellow catfish, H. brachysoma and characterized. This HBF cell line was maintained in Leibovitz's-15 medium supplemented with 15 % fetal bovine serum (FBS) and subcultured more than 62 times over a period of 20 months. ...
Hirakawa Yumi Y Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha Kashiwa, Chiba, 277-8562, - - 2014
Vacuoles are suggested to play crucial roles in plant defense-related cell death. During programmed cell death, previous live cell imaging studies have observed vacuoles to become simpler in structure and have implicated this simplification as a prelude to the vacuole's rupture and consequent lysis of the plasma membrane. Here, we ...
Meng Qing-Bin QB Department of General Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China; Department of Gastrointestinal Surgery,the First Hospital of Wuhan,Wuhan - - 2014
Objective To investigate the effects of eukaryotic translation initiation factor 5A2(EIF5A2)down-regulation by small interfering RNA(siRNA)on aggressiveness of human gastric cancer cell and its potential mechanisms. Methods The expressions of EIF5A2 in human gastric cancer cell lines(MKN28 and HGC27)and immortalized gastric mucosal epithelial cells(GES-1)were measured by real-time quantitative reverse transcription polymerase ...
Sun A A Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, - - 2014
A new ovarian cell line, CSO, was established from half-smooth tongue sole Cynoglossus semilaevis. Primary culture of CSO cells was initiated from digestion of ovarian tissues pieces by trypsin solution and cultured at 24° C in Dulbecco's modified Eagle's medium-F12 medium (DMEM-F12, 1:1) (pH 7·0), supplemented with 20% foetal bovine ...
Fang Su-Chiung SC Academia Sinica; - - 2014
We previously identified a mutation, smt15-1, that partially suppresses the cell cycle defects caused by loss of the retinoblastoma tumor suppressor related protein (RBR) encoded by the MAT3 gene in Chlamydomonas reinhardtii. smt15-1 single mutants were also found to have a cell cycle defect leading to a small-cell phenotype. SMT15 ...
Zhang Hong-Fei HF State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing - - 2014
Objective To investigate the role of TXNDC5 in serum starvation-induced proliferation inhibition of HeLa cell. Methods TXNDC5 was either over-expressed or knocked down by small interfering RNA(siRNA)in HeLa cells which were then cultured in conventional medium or serum starvation medium. The protein level of TXNDC5 was evaluated by Western blot ...
Wan Jiawei J School of Physics and Technology, Wuhan University, Wuhan, Hubei, 430072, P.R. China; CAS Laboratory for Nanosystem and Hierarchy Fabrication, National Center for Nanoscience and Technology, Beijing, 100190, P.R. - - 2014
Pt-Ni alloy nanoparticles are synthesized and used as counter electrodes in dye-sensitized solar cells (DSSCs) for the first time. A PCE of 9.15% is achieved with the Pt3 Ni counter electrode, displaying an evident improvement compared with the conventional pure Pt (8.33%). The cell stability is also obviously increased with ...
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