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Hou Chunfeng C Department of Rheumatology, Jining First people's Hospital, Jining, Shandong 272111, PR - - 2014
MicroRNA let-7i is up-regulated in T cells from patients with Ankylosing Spondylitis (AS). In this study, we investigated the role of let-7i in T cells survival. Our results demonstrated down-regulation of insulin-like growth factor-1 receptor (IGF1R) in T cells from patients with AS. Luciferase reporter assay suggested IGF1R as direct ...
Nelson Charles C Department of Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, - - 2014
Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-14 as being both necessary ...
Kim In Gyu IG Department of Radiation Biology, Environment Radiation Research Group, Korea Atomic Energy Research Institute, Yuseong, Daejeon 305‑600, Republic of - - 2014
Various mechanisms have been proposed to underlie the cellular activity of genistein, based on biological experiments and epidemiological studies. The present study demonstrated that genistein inhibited the expression of cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP)‑dependent isocitrate dehydrogenase (cICDH), thus increasing levels of intracellular reactive oxygen species (ROS) in human promyeloid ...
Poukkula Minna M Institute of Biotechnology, University of Helsinki, P.O. Box 56, 00014 Helsinki, - - 2014
Lamellipodia are dynamic actin-rich cellular extensions that drive advancement of the leading edge during cell migration [1-3]. Lamellipodia undergo periodic extension and retraction cycles [4-8], but the molecular mechanisms underlying these dynamics and their role in cell migration have remained obscure. We show that glia-maturation factor (GMF), which is an ...
Li Xuefeng X Institute of Bioengineering, Zhejiang University of Technology, Chaowang Road 18, P. R. China 310014. zhuq@zjut.edu.cn - - 2014
Novel FRET-based small molecule probes targeting monoamine oxidases (MAOs) were designed and synthesized. These "Turn-ON" fluorescent probes were proved to be capable of directly reporting MAO activities in live mammalian cells.
Bronietzki Alisha W AW 1] Systems-Oriented Immunology and Inflammation Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany [2] Institute for Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, - - 2014
Autophagy is a vital catabolic process for degrading bulky cytosolic contents, which cannot be resorbed via the proteasome. First described as a survival mechanism during nutrient starvation conditions, recent reports have demonstrated that autophagy supports metabolic functions of T cells at various stages of maturation and effector function. Autophagy is ...
Weng Wen-Chin WC Department of Pediatrics, College of Medicine, National Taiwan University Hospital and National Taiwan University, Taipei, - - 2014
Calreticulin (CRT) has been previously correlated with the differentiation of neuroblastoma (NB), implying a favorable prognostic factor. Vascular endothelial growth factor (VEGF) has been reported to participate in the behavior of NB. This study investigated the association of CRT and VEGF-A in NB cells. The expressions of VEGF-A and HIF-1α, ...
Fu Yuejun Y Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China, - - 2014
Deleterious invasiveness of glioma cells into the normal brain tissue is endorsed by its inherent ability to regulate the receptor-mediated adhesive properties, extracellular matrix degradation and remodeling and elevated secretory ability of metalloproteinase (MMPs) such as MMP-2. By doing so, it will create an intercellular space for the invasion of ...
Li Bo B Department of Mechanical Engineering, Biomedical Engineering and Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, - - 2014
Cell migration plays a pivotal role in many physiologically important processes such as embryogenesis, wound-healing, immune defense, and cancer metastasis. Although much effort has been directed toward motility of individual cells, the mechanisms underpinning collective cell migration remain poorly understood. Here we develop a collective motility model that incorporates cell ...
Shin Yeon-Kyun YK Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA. Electronic address: - - 2014
The helical protein MLKL inserts into cell membranes and forms a permeation pore therein, resulting in cell death. In this issue of Structure, the article by Su and colleagues reports that helix 6 regulates the opening of the pore formed by preceding core helices.
Zhang Jiwei J Wuxi Oncology Institute, the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China. Contributed equally to this - - 2014
The expression of miR-638 was found downregulated in colorectal carcinoma (CRC) in our previous study. However, the role of miR-638 in CRC remains unknown. The aim of this study was to determine the function and mechanism of miR-638 in CRC. Here, we verified that miR-638 was frequently downregulated in CRC ...
Liu Hongxu H Department of Thoracic Surgery, The First Hospital, China Medical University, Shenyang 110001, China. Electronic address: - - 2014
SCIN (scinderin) is a calcium-dependent actin severing and capping protein. Homologue in zebrafish has been found to be related with cell death. In the present study, we found that SCIN is highly expressed in human lung cancer specimens. However, the role of SCIN in lung cancer has not yet been ...
Rosendahl Ann H AH Lund University, Department of Clinical Sciences Lund, Division of Surgery, Lund, Sweden; Lund University and Skåne University Hospital, Department of Clinical Sciences Lund, Division of Oncology and Pathology, Lund, Sweden. Electronic address: - - 2014
Pancreatic stellate cells (PSCs) play a key role in the dense desmoplastic stroma associated with pancreatic ductal adenocarcinoma. Studies on human PSCs have been minimal due to difficulty in maintaining primary PSC in culture. We have generated the first conditionally immortalized human non-tumor (NPSC) and tumor-derived (TPSC) pancreatic stellate cells ...
Li Bo B College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA; Department of Tea Science, Zhejiang University, Hangzhou 310058, - - 2014
Adverse side effects and acquired resistance to conventional platinum based chemotherapy have become major impediments in ovarian cancer treatment, and drive the development of more selective anticancer drugs. Chaetoglobosin K (ChK) was shown to have a more potent growth inhibitory effect than cisplatin on two cisplatin-resistant ovarian cancer cell lines, ...
Ni Chao C Cancer Institute(Key Laboratory of Cancer Prevention & Intervention, National Ministry of Education, Provincial Key Laboratory of Molecular Biology in Medical Sciences), the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; Department of General surgery, Zhejiang Provincial People's Hospital, - - 2014
Increasing evidence showed invariant NKT cells (iNKT cell) are an attractive candidate for cancer immunotherapy, but its role in colorectal cancer treatment was still unclear. Here we reported iNKT cells exerted moderate cytotoxic effect against colorectal cancer cells (CRC cells) with the stimulation of α-Galcer, and the mutual recognition between ...
Cho Jin Hyoung JH Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Republic of - - 2014
Esculetin (6,7-dihydroxycoumarin), a coumarin compound, is known to inhibit proliferation and induce apoptosis in several types of human cancer cells and is regarded as a promising chemotherapeutic agent. The purpose of the present study was to investigate the anti-proliferative effects of esculetin on two oral squamous cell carcinoma (OSCC) cell ...
Li Yingchun Y Department of Pathology, University of California San Diego, La Jolla, California; Department of Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, - - 2014
Proper differentiation of placental epithelial cells, called trophoblast, is required for implantation. Early during placentation, trophoblast cell columns help anchor the developing embryo in the uterine wall. Although proximally continuous with villous cytotrophoblast (CTB) distally, these cells differentiate into invasive extravillous trophoblast. We previously reported that p63, a p53 family ...
Choedon Tenzin - - 2014
Yukyung karne (YK) is a traditional Tibetan formulation used for many centuries for the treatment of ovarian cancer. However, the pharmacological basis of its anticancer property is not well understood. In the present study, the anticancer property of YK was investigated in cell culture. The growth inhibitory property of YK ...
Abengózar-Vela Antonio A Instituto de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid , Valladolid , Spain , - - 2014
Abstract Purpose: To develop an in vitro method to determine the protective effect of UV-blocking contact lenses (CLs) in human corneal epithelial (HCE) cells exposed to UV-B radiation. Materials and Methods: SV-40-transformed HCE cells were covered with non-UV-blocking CL, UV-blocking CL or not covered, and exposed to UV-B radiation. As ...
Zhang Yuanqing - - 2014
Chemotaxis is the phenomenon by which the migration and invasion of cells is directed in response to an extracellular chemical gradient. Chemotaxis of tumor cells and tumor-associated inflammatory and stromal cells is mediated by chemokines, chemokine receptors, growth factors and growth factor receptors. Current techniques used to study chemotactic driven ...
Liu Ting-Ting TT 1] Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine (Anhui Medical University), Ministry of Education, Hefei 230032, China [2] Department of Liver Diseases, 123th Hospital of PLA of China, Bengbu 233015, - - 2014
Aim:To explore the effects of noradrenaline (NA) on hepatic stellate cells (HSCs) in vitro and to determine the adrenoceptor (AR) subtypes and underlying mechanisms.Methods:The distribution and expressions of α1A-, α1B-, and α1D-ARs in HSC-T6 cells were analyzed using immunocytochemistry and RT-PCR. Cell proliferation was evaluated with MTT assay. The expression ...
Marble Hetal D - - 2014
Mesenchymal stem cells have been increasingly used for cell-based therapies. Adipose-derived stem/stromal cells (ASCs) from the stromal vascular fraction (SVF) of fat tissue are a particularly attractive option for cell based therapy given their accessibility and relative abundance. However, their application in both clinical and basic science investigations is complicated ...
Carotta Sebastian S The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia Department of Medical Biology, Department of Pathology, Department of Microbiology and Immunology, and Department of Computing and Information Systems, University of Melbourne, Parkville, Victoria 3010, - - 2014
Activated B cells undergo immunoglobulin class-switch recombination (CSR) and differentiate into antibody-secreting plasma cells. The distinct transcriptomes of B cells and plasma cells are maintained by the antagonistic influences of two groups of transcription factors: those that maintain the B cell program, including BCL6 and PAX5, and plasma cell-promoting factors, ...
Grötsch Bettina B Division of Molecular Immunology, Nikolaus Fiebiger Center, Department of Internal Medicine III, Department of Radiation Oncology, Division of Ophthalmology, Department Kopfklinik, University of Erlangen-Nuremberg, D91054 Erlangen, - - 2014
The cornerstone of humoral immunity is the differentiation of B cells into antibody-secreting plasma cells. This process is tightly controlled by a regulatory gene network centered on the transcriptional repressor B lymphocyte-induced maturation protein 1 (Blimp1). Proliferation of activated B cells is required to foster Blimp1 expression but needs to ...
Maul Robert W RW Laboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD - - 2014
Variable (V) genes of immunoglobulins undergo somatic hypermutation by activation-induced deaminase (AID) to generate amino acid substitutions that encode antibodies with increased affinity for antigen. Hypermutation is restricted to germinal center B cells and cannot be recapitulated in ex vivo-activated splenic cells, even though the latter express high levels of ...
Brooks Eric R ER Department of Molecular Biosciences and the Institute for Cell and Molecular Biology, the University of Texas at Austin, Patterson Labs, 2401 Speedway, Austin, TX 78712, USA. Electronic address: - - 2014
Cilia are microtubule-based projections that serve a wide variety of essential functions in animal cells. Defects in cilia structure or function have recently been found to underlie diverse human diseases. While many eukaryotic cells possess only one or two cilia, some cells, including those of many unicellular organisms, exhibit many ...
Shin Nah-Young NY Department of Medicine, Harvard Medical School, Boston, MA 02115 Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA - - 2014
Cell-cell fusion is an evolutionarily conserved process that leads to the formation of multinucleated myofibers, syncytiotrophoblasts and osteoclasts, allowing their respective functions. Although cell-cell fusion requires the presence of fusogenic membrane proteins and actin-dependent cytoskeletal reorganization, the precise machinery allowing cells to fuse is still poorly understood. Using an inducible ...
Quan Yudong - - 2014
Attempts to eradicate HIV from cellular reservoirs are vital but depend on a clear understanding of how viral variants are transmitted and survive in the different cell types that constitute such reservoirs. Mutations in the env gene of HIV may be able to exert a differential influence on viral transmission ...
Li Jing-Ting JT College of Resource and Environment Science, Pingdingshan University, Pingdingshan 467000, China. Electronic address: - - 2014
Mesenchymal stem cells (MSCs) have been reported as an attractive source for the generation of transplantable surrogate β cells. The objective of this study was to investigate a new method to induce the differentiation of hMSCs into insulin secretion cells and to explore its molecular mechanisms. In this study, we ...
Garipov Azat R AR Department of Microbiology, Kazan Federal (Volga-Region) University, Kremlevskaya str. 18, Kazan, Russia - - 2014
The cytotoxic effects of Bacillus intermedius RNase (binase) towards ovarian cancer cells (SKOV3 and OVCAR5) were studied in comparison to normal ovarian epithelial cells (HOSE1 and HOSE2). Binase decreased viability and induced the selective apoptosis of ovarian cancer cells. The apoptosis rate was 50% in SKOV3 and 48% in OVCAR5 ...
Pelipenko Jan J University of Ljubljana, Faculty of Pharmacy, Ljubljana, - - 2014
Electrospun polymer nanofibers have opened new opportunities in the rapidly evolving field of tissue engineering, particularly due to their topography and variability of available biomaterials. In order to better understand nanofiber influence on cell growth, the impact of their diameter was systematically examined. In this study homogenous, randomly oriented poly(vinyl ...
Xie Qi-Chao - - 2014
Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-beta-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines. In our present study, ...
Berod Luciana L 0000000296773483 Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover, - - 2014
Interleukin-17 (IL-17)-secreting T cells of the T helper 17 (TH17) lineage play a pathogenic role in multiple inflammatory and autoimmune conditions and thus represent a highly attractive target for therapeutic intervention. We report that inhibition of acetyl-CoA carboxylase 1 (ACC1) restrains the formation of human and mouse TH17 cells and ...
Liu Runjing R Duke University, Durham, NC, - - 2014
The primary sensory feature represented within the rodent barrel cortex is the velocity with which a whisker has been deflected. Whisker deflection velocity is encoded within the thalamus via population synchrony (higher deflection velocities entail greater synchrony among the corresponding thalamic population). Thalamic (TC) cells project to regular spiking (RS) ...
Tang Bo B Department of Hepatobiliary Surgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, Guangxi, People's Republic of - - 2014
To investigate the impact of histone methyltransferase enhancer of zeste homolog 2 (EZH2) on the proliferation and apoptosis of human cholangiocarcinoma cells as well as its related mechanisms. Immunohistochemistry and Western blot analyses were used to examine the expression of EZH2 in 40 cases of human cholangiocarcinoma tissues and four ...
Wei Gaijie G Department of Urology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, - - 2014
To prepare polycaprolactone (PCL)/silk fibroin/collagen electrospun nanofiber scaffold and test its effects on growth and proliferation of oral mucosal epithelial cells. Regenerated silk fibroin film, water-soluble collagen powder, and PCL, at mass ratios of 1:1:4, 1:1:8, and 1:1:10, were dissolved in hexafluoroisopropanol, and electrostatic spinning method was adopted to prepare ...
Kimira Yoshifumi Y Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. Electronic address: - - 2014
Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly ...
Zhang Xi X Toxicology Institute, College of Preventive Medicine, Third Military Medical University, No. 30, Gaotanyan Road, Shapingba District, Chongqing 400038, People's Republic of China. Electronic address: - - 2014
Dibutyl phthalate (DBP) is a peroxisome proliferator which can lead to germ cell loss from Sertoli cells. Collapse of vimentin filaments occur in Sertoli cells after DBP exposure. Peroxisome proliferator activated receptor α (PPARα) is a key receptor which could be activated by DBP. The role of PPARα in this ...
Noguchi S S Laboratory of Molecular Diagnostic and Therapeutics, Joint faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, - - 2014
MicroRNA (miR)-203 is downregulated and acts as an anti-oncomir in melanoma cells. Here, using human and canine melanoma cells, we elucidated the effects of miR-203 on cyclic adenosine monophosphate response element binding protein (CREB)/microphthalmia-associated transcription factor (MITF)/RAB27a pathway, which is known to be important for the development and progression of ...
Weaver Jessica R JR Department of Microbiology and Molecular Cell Biology, LH 2128, Eastern Virginia Medical School, 700 W. Olney Road, Norfolk, VA, 23507, - - 2014
Upregulation of the reactive oxygen species (ROS)-producing enzyme NADPH oxidase (NOX)-1 in islets and beta cells follows acute exposure to inflammatory cytokines and is concomitant with beta cell dysfunction. NOX-1 is a candidate mediator of inflammation-induced beta cell dysfunction. This study aimed to determine whether selective inhibition of NADPH oxidase-1 ...
Wilkowska Agnieszka A Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Lodz, - - 2014
The aim of this research was to study how the yeast cell immobilization technique influences the growth and fermentation profiles of Kluyveromyces marxianus cultivated on apple/chokeberry and apple/cranberry pomaces. The encapsulation of cells was performed by droplet formation from a foamed alginate solution. The growth and metabolic profiles were evaluated ...
Vágnerová Lenka L Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Prague, Czech - - 2014
Cells belonging to the germ lineage segregate physically and molecularly from their somatic neighbors during embryogenesis. While germ line-specific chromatin modifications have been identified at later stages in the Caenorhabditis elegans nematode, none have been found in the single P4 germ line founder cell that arises at the beginning of ...
Zhang Xin X Research Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650224, Yunnan, - - 2014
The first continuous cell line from the neonate larval tissues of Blaps rhynchoptera, which has been used as a folk medicine in Yunnan Province, China, was established and designated RIRI-BR1. This cell line was serially subcultured in Schneider's medium supplemented with 15% fetal bovine serum (FBS). The cells grew adherent ...
Sung Sang Do SD Department of Chemistry and Chemical Engineering, Inha University, Incheon 402-751, Korea. - - 2014
Three novel carbazole-based molecules have been synthesized and successfully applied as hole-transporting materials (HTMs) of CH3NH3PbI3-based perovskite solar cells. In particular, the perovskite cell with SGT-405, having a three-arm-type structure, exhibited a remarkable photovoltaic conversion efficiency (PCE) of 14.79%.
Fattouh Ramzi R Cell Biology Program, Hospital for Sick Children, Toronto, ON, M5G0A4, - - 2014
The Gram-positive bacterium Listeria monocytogenes is a facultative intracellular pathogen whose virulence depends on its ability to spread from cell-to-cell within an infected host. Although Arp2/3 is necessary and sufficient for Listeria actin tail assembly, previous studies suggest that other actin polymerization factors, such as formins, may participate in protrusion ...
Suematsu Tomohisa T Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, - - 2014
Mitotic catastrophe, a form of cell death that occurs during mitosis and after mitotic slippage to a tetraploid state, plays an important role in the efficacy of cancer cell killing by microtubule inhibitors. Prolonged mitotic arrest at the spindle assembly checkpoint (SAC) is a well-known requirement for mitotic catastrophe and, ...
Yue Hongwei H Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, - - 2014
Sphingosylphosphorylcholine (SPC) is a bioactive lipid mediated popular cell apoptosis in cancer cells. As a cell-specific sphingolipid, its function in lung cancer cells is unknown. Here we showed that SPC treatment triggered necrosis and autophagy but inhibited apoptosis in two non-small cell lung cancer cell lines: A549 cell line and ...
Parzonko Andrzej A Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland. Electronic address: - - 2014
In this study, the growth-inhibitory effect of polysaccharide (1,3)(1,4)-β-D-glucan from oat, Avena sativa L. grains was explored on the human skin melanoma HTB-140 cells in vitro. The oat β-D-glucan (OBG) exerted cytotoxic action on HTB-140 cells. After 24h of incubation, LD50 (concentration at which 50% of the cells were found ...
Zineb Boumart B Institut National de la Recherche Agronomique, UMR1282 Infectiologie et Santé Publique, F-37380, Nouzilly, France; Université François Rabelais, UMR1282 Infectiologie et Santé Publique, F-37000, Tours, France; Agence Nationale de Sécurité Sanitaire de l'alimentation, de l'environnement et du travail, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Plouragan, - - 2014
Salmonella is a facultative intracellular bacterium found within a variety of phagocytic and non-phagocytic cells in vitro and in vivo. For decades, it has been accepted that Salmonella can enter cells only through a Trigger mechanism mediated by a type three secretion system, called T3SS-1. However, recent researches have shown ...
Knox J Paul JP Centre for Plant Sciences, School of Biology, University of Leeds, Leeds LS2 9JT, United - - 2014
In plants, the intercellular transport of simple and complex molecules can occur symplastically through plasmodesmata. These are membranous channels embedded in cell walls that connect neighbouring cells. The properties of the cell walls surrounding plasmodesmata determine their transport capacity and permeability. These cell wall micro-domains are enriched in callose and ...
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