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Wichmann Henri H Department of Radiotherapy, Martin-Luther-University Halle-Wittenberg, Halle, Germany, - - 2014
The epidermal growth factor receptors, EGFR (HER1) and HER2, have proven prognostic relevance in a variety of human malignancies and both are functionally involved in the molecular pathogenesis of malignant gliomas. We selectively inhibited EGFR and HER2 in glioblastoma cell lines via EGFR- and HER2-specific siRNAs and through the binding ...
Yin Ke K Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara, 630-0192, - - 2014
Visualization of the spatiotemporal pattern of cell division is crucial to understanding how multicellular organisms develop and how they modify their growth in response to varying environmental conditions. The mitotic cell cycle consists of four phases: S (DNA replication), M (mitosis and cytokinesis), and the intervening G1 and G2 phases; ...
Li Nianyu N Department of Comparative Biology and Safety Sciences, Amgen, Amgen Court West 1201, Seattle, WA - - 2014
Mitochondrial perturbation has been recognized as a contributing factor to various drug-induced organ toxicities. To address this issue, we developed a high-throughput flow cytometry-based mitochondrial signaling assay (CMSA) to systematically investigate mitochondrial/cellular parameters known to be directly impacted by mitochondrial dysfunction: mitochondrial membrane potential (MMP), mitochondrial reactive oxygen species (ROS), ...
Yoo Sally - - 2014
Aberrational epigenetic marks are believed to play a major role in establishing the abnormal features of cancer cells. Rational use and development of drugs aimed at epigenetic processes requires an understanding of the range, extent, and roles of epigenetic reprogramming in cancer cells. Using ChIP-chip and MeDIP-chip approaches, we localized ...
Kesarwani Pravin P Department of Surgery, Medical University of South - - 2014
Ex vivo-expanded CD8+ T cells used for adoptive immunotherapy generally acquire an effector memory-like phenotype (TEM cells). With regard to therapeutic applications, two undesired features of this phenotype in vivo are limited persistence and reduced anti-tumor efficacy, relative to CD8+ T cells with a central memory-like phenotype (TCM cells). Further, ...
Lu Jing J Department of Radiation Oncology, the First Affiliated Hospital of Nanjing Medical University, - - 2014
IAP antagonists increased the antitumor efficacy of X-irradiation in some types of cancers, but their effects on hypoxic cancer cells remain unclarified. We aims to investigate the radiosensitizing effect of an IAP inhibitor AT-406 on cervical cancer cell lines under both normoxia and hypoxia conditions. Hela and Siha cells were ...
Veneault-Fourrey Claire C INRA, Interactions Arbres - Microorganismes, UMR1136, F-54280 Champenoux, France; Université de Lorraine, Interactions Arbres - Microorganismes, UMR1136, F-54500 Vandoeuvre-lès-Nancy, France. Electronic address: - - 2014
Ectomycorrhizal fungi, living in soil forests, are required microorganisms to sustain tree growth and productivity. The establishment of mutualistic interaction with roots to form ectomycorrhiza (ECM) is not well known at the molecular level. In particular, how fungal and plant cell walls are rearranged to establish a fully functional ectomycorrhiza ...
Guo Xuesong X Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, - - 2014
Extracellular polymeric substances (EPS) are susceptible to contamination by intracellular substances released during the extraction of EPS owing to the damage caused to microbial cell structures. The damage to cell walls and cell membranes in nine EPS extraction processes of activated sludge was evaluated in this study. The extraction of ...
Wang Zhu A ZA Departments of Medicine, Genetics and Development, Urology, and Systems Biology, Columbia Stem Cell Initiative, Herbert Irving Comprehensive Cancer Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, - - 2014
The identification of cell types of origin for cancer has important implications for tumor stratification and personalized treatment. For prostate cancer, the cell of origin has been intensively studied, but it has remained unclear whether basal or luminal epithelial cells, or both, represent cells of origin under physiological conditions in vivo. ...
Kwolek-Mirek Magdalena M Department of Biochemistry and Cell Biology, University of Rzeszow, - - 2014
Determination of cell viability is the most commonly used method for assessing the impact of various types of stressors in toxicity research and in industrial microbiology studies. Viability is defined as a percentage of live cells in a whole population. Although cell death is one of the consequences of toxicity, ...
McCarron Mark J - - 2014
T follicular helper (Tfh) cells contribute to the establishment of humoral immunity by controlling the delivery of helper signals to activated B cells; however, Tfh development must be restrained, as aberrant accumulation of these cells is associated with positive selection of self-reactive germinal center B cells and autoimmunity in both ...
Pupovac Aleta A 1] School of Biological Sciences, University of Wollongong, Wollongong, New South Wales, Australia [2] Illawarra Health and Medical Research Institute, Wollongong, New South Wales, - - 2014
Activation of the P2X7 receptor by the extracellular damage-associated molecular pattern, adenosine 5'-triphosphate (ATP), induces the shedding of cell surface molecules including the low-affinity IgE receptor, CD23, from human leukocytes. A disintegrin and metalloprotease (ADAM) 10 mediates P2X7-induced shedding of CD23 from multiple myeloma RPMI 8226 B cells; however, whether ...
Lin Tzu-Huai TH National Yang-Ming - - 2014
The Hippo pathway is a key signaling cascade in controlling organ size. The core components of this pathway are two kinases, Hippo (Hpo) and Warts (Wts), and a transcriptional coactivator Yorkie (Yki). YAP (a Yki homolog in mammals) promotes epithelial-mesenchymal transition and cell migration in vitro. Here, we use border ...
Belatto Maria Fernanda da Silva MF Postgraduate Program in Dentistry, Universidade Federal de Santa Catarina, Florianópolis, SC, - - 2014
The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to ...
Oei Gezina T M L GT Department of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A.), Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands - - 2014
Helium, a noble gas, has been safely used in humans. In animal models of regional myocardial ischemia/reperfusion it was shown that helium conditioning reduces infarct size. Currently it is not known how helium exerts its cytoprotective effects and which cell death/survival pathways are affected. The objective of this study was ...
Bellenberg Sören S Universität Duisburg-Essen, Biofilm Centre, Universitätsstraße. 5, 45141 Essen, Germany. Electronic address: - - 2014
Bioleaching of metal sulfides is an interfacial process where biofilm formation is considered to be important in the initial steps of this process. Among the factors regulating biofilm formation, molecular cell-to-cell communication such as quorum sensing is involved. A functional LuxIR-type I quorum sensing system is present in Acidithiobacillus ferrooxidans. ...
Anderson Angela S AS Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, - - 2014
An altered metabolism during ovarian cancer progression allows for increased macromolecular synthesis and unrestrained growth. However, the metabolic phenotype of cancer stem or tumor-initiating cells, small tumor cell populations that are able to recapitulate the original tumor, has not been well characterized. In the present study, we compared the metabolic ...
Li Yao-Yin YY Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, PR - - 2014
The transcriptional factor Snail has been reported to possess properties related to cancer progression; however, the mechanism for it is not fully understood. Our data showed that Snail knockdown by small interfering RNA in two OSCC cell lines, WSU-HN6 and CAL27, significantly inhibited cell migration and invasion which also resulted ...
Jackson Simon A SA Department of Biochemistry, University of Otago, Dunedin 9016, New - - 2014
The Photosystem II (PS II) assembly factors Psb27 and Ycf48 are transiently associated with PS II during its biogenesis and repair pathways. We investigated the function of these proteins by constructing knockout mutants in Synechocystis sp. PCC 6803. In ΔYcf48 cells, PS II electron transfer and stable oxygen evolution were ...
Son Young-Ok YO University of Kentucky, United - - 2014
The cadmium-transformed human lung bronchial epithelial BEAS-2B cells exhibit a property of apoptosis resistance as compared to normal non-transformed BEAS-2B cells. The level of basal reactive oxygen species (ROS) is extremely low in transformed cells in correlation with elevated expressions of both anti-oxidant enzymes (catalase, SOD1, and SOD2) and anti-apoptotic ...
Harvey Cameron W CW Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, Notre Dame, IN, 46556; USA Department of Physics, University of Notre Dame, Notre Dame, IN, 46556; - - 2014
Links between cell division and other cellular processes are poorly understood. It is difficult to simultaneously examine division and function for most cell types. Most research probing aspects of cell division has experimented with stationary or immobilized cells, or distinctly asymmetrical cells. Here we take an alternative approach by examining ...
Saier Milton H MH Jr Department of Molecular Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116 - - 2014
Holins form pores in the cytoplasmic membranes of bacteria for the primary purpose of releasing endolysins that hydrolyze the cell wall and induce cell death. Holins are encoded within bacteriophage genomes where they promote cell lysis for virion release and within bacterial genomes where they serve a diversity of potential ...
Schuetz Mathias M Department of Botany, University of British Columbia, Vancouver, - - 2014
Plants precisely control lignin deposition in spiral or annular secondary cell wall domains during protoxylem tracheary element (TE) development. Because protoxylem TEs function to transport water within rapidly elongating tissues, it is important that lignin deposition is restricted to the secondary cell walls, in order to preserve the plasticity of ...
Huijskens Mirelle J A J MJ Department of Internal Medicine, Division of Hematology, Maastricht University Medical Center, and Department of Toxicogenomics, School for Oncology and Developmental Biology, Maastricht University, Maastricht, the Netherlands - - 2014
The efficacy of donor HSCT is partly reduced as a result of slow post-transplantation immune recovery. In particular, T cell regeneration is generally delayed, resulting in high infection-related mortality in the first years post-transplantation. Adoptive transfer of in vitro-generated human T cell progenitors seems a promising approach to accelerate T ...
Bird Sara Whitney SW Departments of Microbiology and Immunology - - 2014
The cell-to-cell spread of cytoplasmic constituents such as nonenveloped viruses and aggregated proteins is usually thought to require cell lysis. However, mechanisms of unconventional secretion have been described that bypass the secretory pathway for the extracellular delivery of cytoplasmic molecules. Components of the autophagy pathway, an intracellular recycling process, have ...
Fouchard Jonathan J Laboratoire Matière et Systèmes Complexes, Unité Mixte de Recherche 7057, Centre National de la Recherche Scientifique and Université Paris-Diderot, 75205 Paris, - - 2014
Cell shape affects proliferation and differentiation, which are processes known to depend on integrin-based focal adhesion (FA) signaling. Because shape results from force balance and FAs are mechanosensitive complexes transmitting tension from the cell structure to its mechanical environment, we investigated the interplay between 3D cell shape, traction forces generated ...
Gibellini Lara L Department of Surgery, Medicine, Dentistry, and Morphological Sciences, and Department of Life Sciences, University of Modena and Reggio Emilia, Modena, - - 2014
Lon is a nuclear-encoded, mitochondrial protease that assists protein folding, degrades oxidized/damaged proteins, and participates in maintaining mtDNA levels. Here we show that Lon is up-regulated in several human cancers and that its silencing in RKO colon cancer cells causes profound alterations of mitochondrial proteome and function, and cell death. ...
de Oliveira Alane Cabral AC Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceio, Al, 57072-970 (Brazil); Faculdade de Nutrição, Universidade Federal de Alagoas - - 2014
The aim of this work was to investigate the mechanism of action of 2-ferrocenyl-1,1-diphenylbut-1-ene (1) on HL-60 human leukemia cells. While inactive against noncancerous cells, 1 provoked a concentration-dependent decrease in viable tumor cells, primarily via apoptosis, as evidenced by analysis of cell morphology, activation of caspases 3 and 7, increased ...
Shalhout Sophia S Department of Chemistry, Wayne State University, Detroit, MI 48202, - - 2014
AID converts DNA-cytosines to uracils in immunoglobulin genes, creating antibody diversification. It also causes mutations and translocations that promote cancer. We examined the interplay between uracil creation by AID and its removal by UNG2 glycosylase in splenocytes undergoing maturation, and in B-cell cancers. The genomic uracil levels remain unchanged in ...
Keeley Patrick W PW Neuroscience Research Institute and Department of Psychological and Brain Sciences, University of California at Santa Barbara, Santa Barbara, CA - - 2014
Programmed cell death contributes to the histogenesis of the nervous system, and is believed to be modulated through the sustaining effects of afferents and targets during the period of synaptogenesis. Cone bipolar cells undergo programmed cell death during development, and we confirm that the numbers of three different types are ...
Walia Rupali R Faculty of Veterinary Medicine, University of Calgary, Alberta, - - 2014
Studies have shown that Porcine circovirus (PCV)-2 induces apoptosis in PK15 cells. Here we report that cell death is induced in PCV2b-infected PK15 cells that express Capsid (Cap) protein and this effect is enhanced in interferon gamma (IFN-γ)-treated cells. We further show that transient PCV2a and 2b-Cap protein expression induces ...
Yang Xiaojing X Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu, 226001, People's Republic of - - 2014
Chromosome region maintenance 1 (CRM1) has been related to several malignancies. The predictive value of CRM1 in the malignance and prognosis of esophageal squamous cell carcinoma (ESCC), however, is not clear yet. In this study, we displayed that CRM1 expression was up-regulated in ESCC using immunohistochemistry and Western blot. Statistical ...
de Souza Ludmilla - - 2014
BackgroundSelol is an oily mixture of selenitetriacylglycerides that was obtained as a semi-synthetic compound containing selenite. Selol is effective against cancerous cells and less toxic to normal cells compared with inorganic forms of selenite. However, Selol¿s hydrophobicity hinders its administration in vivo. Therefore, the present study aimed to produce a ...
Pastorek M - - 2014
The aim of the present study was to compare the effect of realgar nanoparticles and arsenic trioxide (ATO) on viability, DNA damage, proliferation, autophagy and apoptosis in the human melanoma cell lines BOWES and A375. The application of various flow cytometric methods for measurements cell viability, DNA cell cycle, mitochondrial ...
Gao C - - 2014
Clear-cell renal cell carcinoma is a highly treatment-resistant tumor type. Heme oxygenase-1 plays an anti-apoptotic role in cancer chemotherapeutic inducing tumor-progression. The miR-200 family was involved in the process of mesenchymal-epithelial transition (MET) during renal development. In the present study, we demonstrated the regulatory relationship between miR-200c and HO-1. We provided ...
Pradhan Arun Kumar AK Academy of Scientific and Innovative Research, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India, - - 2014
Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The ...
Radojevic Vesna V Department of Biomedicine, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland, - - 2014
Somatostatin (SST) is a peptide hormone that exerts inhibitory effects mediated through binding to specific cell surface G protein-coupled receptors, of which five distinct subtypes (SSTR1-SSTR5) have been characterized. Our study performed on mouse cochlear hair cells shows the expression and localization of the three receptors (SSTR3-SSTR5) in wild-type (WT), ...
Aumsuwan Pranapda P National Center for Natural Product Research, School of Pharmacy, University of Mississippi, University, MS, 38677, - - 2014
The present study was designed to evaluate the efficacy of wild yam root extract (WYRE) as a potential demethylating agent using two breast cancer cell lines, MCF-7 (estrogen receptor positive; ER(+)) and MDA-MB-231 (Estrogen receptor negative; ER(-)), and a methylated gene, GATA3, as a potential marker of breast cancer development. ...
Wu Yuanhao Y Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, 200040, - - 2014
Although long non-coding RNAs (lncRNAs) are known to play an important role in cell regulation in several cancers, the regulatory mechanisms in renal carcinoma cells remain unclear. HOX transcript antisense RNA (HOTAIR), an lncRNA, coordinates with chromatin-modifying enzymes to regulate gene silencing. HOTAIR is over-expressed in several types of carcinoma ...
Martin Ciara A CA UCLA Interdepartmental Program in Molecular Toxicology. Electronic address: - - 2014
The neurodegenerative effects of Parkinson's disease (PD) are marked by a selective loss of dopaminergic (DA) neurons. Epidemiological studies suggest that chronic exposure to the pesticide paraquat may increase the risk for PD and DA cell loss. However, combined exposure with additional fungicide(s) including maneb and/or ziram may be required ...
Lee Chi-Chiu CC Department of Food Science and Center of Excellence for the Ocean, National Taiwan Ocean University. 2 Pei-Ning Road, Keelung 202, Taiwan, - - 2014
The crown-of-thorns starfish (Acanthaster planci) is a venomous starfish. In this study, the extraction of A. planci spine venom (ASV) was performed by phosphate saline buffer, followed by assaying the cytotoxicity on human normal and tumor cells. It was found that human melanoma cells (A375.S2) were the most sensitive to ...
Martins Alessandro F AF Grupo de Materiais Poliméricos e Compósitos, GMPC - Departamento de Química. Universidade Estadual de Maringá UEM - Av. Colombo 5790 - CEP 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR),-Estrada para Boa Esperança; CEP 85660-000, Dois Vizinhos, Paraná, Brasil. Electronic address: - - 2014
Polyelectrolyte complex beads based on N,N,N-trimethyl chitosan (TMC) and sodium alginate (ALG) were obtained. This biomaterial was characterised by FTIR, TGA/DTG, DSC and SEM analysis. The good properties of polyelectrolyte complex hydrogel beads were associated, for the first time, with gold nanoparticles (AuNPs). Through a straightforward methodology, AuNPs were encapsulated ...
Huang Chih-Ping CP Sex Hormone Research Center, Departments of Urology/Surgery and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, - - 2014
The urothelium is constantly rebuilt by normal urothelial cells to regenerate damaged tissues caused by stimuli in urine. However, the urothelial carcinoma cells expand the territory by aberrant growth of tumor cells, which migrate and occupy the damaged tissues to spread outside and disrupt the normal cells and organized tissues ...
Xue Zhongfeng Z The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 16, Jichang Road, 510405 Guangzhou, China; Sanya Traditional Chinese Medicine Hospital, 106, Fenghuang Road, 572000 Sanya, China. Electronic address: - - 2014
Alzheimer's disease (AD) is an age related and progressive neurodegenerative disease. Autophagy is a self-degradative process and plays a critical role in removing long-lived proteins and damaged organelles. Recent evidence suggests that autophagy might be involved in the pathogenesis of AD. β-asarone have various neuroprotective effects. However, the effect of ...
Pennington Elliot C EC Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, Mass., - - 2014
Purpose: The proportions of select stem cells in term amniotic fluid have been shown to correlate with the type and size of experimental neural tube defects (NTDs). We sought to determine the impact of gestational age upon this form of targeted amniotic cell profiling. Methods: Sprague-Dawley fetuses with retinoic acid-induced ...
Granier Celine J CJ Child Health Institute of New Jersey, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, NJ 08901, USA rabsonab@rwjms.rutgers.edu - - 2014
PDCD2 (programmed cell death domain 2) is a highly conserved, zinc finger MYND domain-containing protein essential for normal development in the fly, zebrafish and mouse. The molecular functions and cellular activities of PDCD2 remain unclear. In order to better understand the functions of PDCD2 in mammalian development, we have examined ...
Yang Nae-Cherng NC School of Nutrition, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung City, 40201, Taiwan, ROC, - - 2014
Calorie restriction (CR) extends lifespan in a remarkable range of organisms. However, the mechanisms of CR related to the longevity effects are not fully elucidated to date. Using human fibroblast Hs68 (Hs68) cells cultured at a lower level of medium glucose (i.e., glucose restriction; GR) to mimic CR, we investigated ...
Bi Fang-Cheng FC State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R. - - 2014
Arabidopsis thaliana plants that lack ceramide kinase, encoded by ACCELERATED CELL DEATH5 (ACD5), display spontaneous programmed cell death late in development and accumulate substrates of ACD5. Here, we compared ceramide accumulation kinetics, defense responses, ultrastructural features, and sites of reactive oxygen species (ROS) production in wild-type and acd5 plants during ...
Reuther C C Institut für Pharmakologie und Klinische Pharmazie, Fachbereich Pharmazie, Philipps-Universität Marburg, Karl-von-Frisch-Straße 1, 35032, Marburg, Germany, - - 2014
It is well-established that activation of proteases, such as caspases, calpains and cathepsins are essential components in signaling pathways of programmed cell death (PCD). Although these proteases have also been linked to mechanisms of neuronal cell death, they are dispensable in paradigms of intrinsic death pathways, e.g. induced by oxidative ...
Mihaly S R SR Department of Biological Sciences, North Carolina State University, Environmental and Molecular Toxicology, Raleigh, NC 27695-7633, - - 2014
Programmed cell death, a physiologic process for removing cells, is critically important in normal development and for elimination of damaged cells. Conversely, unattended cell death contributes to a variety of human disease pathogenesis. Thus, precise understanding of molecular mechanisms underlying control of cell death is important and relevant to public ...
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