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Results 451 - 500 of 1415
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Kumar Ashwath S AS Comparative Oncology and Epigenetics Laboratory, Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri, United States of - - 2014
In spite of chemotherapeutic and surgical advances, pancreatic cancer continues to have a dismal prognosis. Metastasis due to tumor cell migration remains the most critical challenge in treating pancreatic cancer, and conventional chemotherapy is rarely curative. In the quest for more novel molecules to fight this disease, we tested the ...
Franco Daiane Gil DG Laboratory of Chronopharmacology, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, - - 2014
The constitutive activation of nuclear factor-κB (NF-κB), a key transcription factor involved in neuroinflammation, is essential for the survival of neurons in situ and of cerebellar granule cells in culture. Melatonin is known to inhibit the activation of NF-κB and has a cytoprotective function. In this study, we evaluated whether ...
Liu Jia Ming JM Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, - - 2014
Accumulating studies revealed that the expression levels of several miRNAs are up or down-regulated in osteosarcoma (OS). The aim of this study was to investigate the functional significance and molecular of the let-7g in OS cells. The expression levels of let-7g was significantly down-regulated in OS cell lines U2-OS and ...
Tan Yingxia Y Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang 325000, - - 2014
Thioredoxin-1 (Trx-1), an important redox regulatory factor, plays a significant role in drug-induced apoptosis. Here we investigated the effects of the Trx-1 inhibitor 1-methylpropyl 2-imidazolyl disulfide (PX-12) on human acute myeloid leukemia cells (AML) and the sensitivity of cells to arsenic trioxide (As2O3, ATO). Treatment of cells with a different ...
Nitta Takeo T Department of Human Pathology, Kanazawa University Graduate School of Medicine Kanazawa, Japan ; Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine Sapporo, - - 2014
The role of autophagy in cholangiocarcinoma is poorly understood. This study investigated its involvement in cholangiocarcinoma, focusing on carcinoma cell invasion and prognostic significance using cholangiocarcinoma cell lines, CCKS1 and HuCCT1, and human tissues of hilar and extrahepatic cholangiocarcinoma. Nutrient starvation induced the expression of LC3-II and the formation of ...
Pine Sharon R SR Department of Medicine, Robert Wood Johnson Medical School, Rutgers Cancer Institute of New Jersey, Rutgers, The State University of New Jersey , New Brunswick, NJ , - - 2014
During tissue homeostasis, normal stem cells self-renew and repopulate the diverse cell types found within the tissue via a series of carefully controlled symmetric and asymmetric cell divisions (ACDs). The notion that solid tumors comprise a subset of cancer stem cells (CSCs) with dysregulated self-renewal and excessive symmetric cell divisions ...
Shen Ching-I CI Department of Chemistry, Agricultural Biotechnology Center, National Chung-Hsing University, - - 2014
Accelerating proliferation of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis in embryo, also involves in cell proliferation and tumorigenesis in adult tissues. Here the effects of Wnt signal on the growth of human interfollicular keratinocytes were investigated. We ...
Jorns Carl C Division of Transplantation Surgery, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, - - 2014
Hepatocyte transplantation is an upcoming treatment for patients with metabolic liver diseases. Repeated cell infusions over 1-2 days improve clinical outcome. Isolated hepatocytes are usually cold stored in preservation solutions between repeated infusions. However, during cold storage isolated hepatocytes undergo cell death. We investigated if tissue preservation and repeated isolations ...
Deb Swati Palit SP Department of Biochemistry and Molecular Biology, and the Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, 23298, USA, - - 2014
Frequent overexpression of MDM2 in human cancers suggests that the protein confers a survival advantage to cancer cells. However, overexpression of MDM2 in normal cells seems to restrict cell proliferation. This review discusses the cell growth regulatory functions of MDM2 in normal and genetically defective cells to assess how cancer ...
MacMillan Connor D CD 1] Department of Pathology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON [2] London Regional Cancer Program, London Health Sciences Centre, London, ON [3] Department of Surgery, Schulich School of Medicine and Dentistry, University of Western Ontario, London, - - 2014
Planar cell polarity (PCP) signaling has been shown in different studies to either promote or inhibit the malignancy of breast cancer. Using the 21T cell lines, which were derived from an individual patient and represent distinct stages of progression, we show that the prototypical PCP ligand, WNT5A, is expressed highest ...
Dunnick Katherine M KM a National Institute for Occupational Safety and Health, HELD , Morgantown , West Virginia , - - 2014
Due to their unique size, surface area, and chemical characteristics, nanoparticles' use in consumer products has increased. However, the toxicity of nanoparticle (NP) exposure during the manufacturing process has not been fully assessed. Tungstate NP are used in numerous products, including but not limited to scintillator detectors and fluorescent lighting. ...
Yusuf Mohammed M London Centre for Nanotechnology, University College London, London, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Oxon, United - - 2014
Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished ...
Abenza Juan F JF The Gurdon Institute, University of Cambridge, Cambridge, United - - 2014
Every cell has a characteristic shape key to its fate and function. That shape is not only the product of genetic design and of the physical and biochemical environment, but it is also subject to inheritance. However, the nature and contribution of cell shape inheritance to morphogenetic control is mostly ...
Kapere Ochieng Joshua J Department of Pediatric Surgery of the Erasmus MC-Sophia Children's Hospital, Rotterdam, the - - 2014
The adult lung contains several distinct stem cells, although their properties and full potential are still being sorted out. We previously showed that ectopic Sox2 expression in the developing lung manipulated the fate of differentiating cells. Here, we addressed the question whether fully differentiated cells could be redirected towards another ...
Pemberton Lucy F LF Department of Microbiology, Immunology and Cancer Biology, Center for Cell Signaling, University of Virginia, MSB (Hospital West), Room 7201, 800577, Charlottesville, VA, 22908, USA, - - 2014
In spite of their small size, the cellular morphology, structure, and protein localization of yeast cells can be successfully imaged. A detailed protocol for preparing yeast cells for live-cell imaging is described, including techniques to immobilize yeast for time-lapse microscopy. Protocols for indirect immunofluorescence are outlined, including strategies for fixation, ...
Meng Erhong E University of South Alabama Mitchell Cancer Institute, Mobile, Alabama, United States of - - 2014
Aldehyde dehydrogenase (ALDH) expressing cells have been characterized as possessing stem cell-like properties. We evaluated ALDH+ ovarian cancer stem cell-like properties and their role in platinum resistance. Isogenic ovarian cancer cell lines for platinum sensitivity (A2780) and platinum resistant (A2780/CP70) as well as ascites from ovarian cancer patients were analyzed ...
Kwong Lina L Department of Molecular and Cell Biology, University of California, 16 Barker Hall, 3204, Berkeley, California 94720, - - 2014
The distribution of Sonic Hedgehog (Shh) is a highly regulated and critical process for development. Several negative feedback mechanisms are in place, including the Shh-induced upregulation of Hedgehog-interacting protein (Hhip). Hhip sequesters Shh, leading to a non-cell autonomous inhibition of the pathway. Hhip overexpression has a severe effect on neural ...
Wang Ying Y 2. Department of Pharmacology (Key Laboratory of Cardiovascular Medicine Research, Ministry of Education; State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang 150081, P. R. - - 2014
Preeclampsia is a serious complication in pregnancy. Dysregulation of trophoblast cell proliferation and invasion is a major pathological alteration observed in preeclampsia. Recently, microRNAs were shown to participate in the pathogenesis of preeclampsia. In this study we explored the effect of miR-20a on the proliferation and invasion of trophoblast cells ...
Brudzynski Katrina K Bee-Biomedicals Inc., Drug Discovery and Development Department, St. Catharines, Ontario, - - 2014
Honeys show a desirable broad spectrum activity against Gram-positive and negative bacteria making antibacterial activity an intrinsic property of honey and a desirable source for new drug development. The cellular targets and underlying mechanism of action of honey antibacterial compounds remain largely unknown. To facilitate the target discovery, we employed ...
Möller Yvonne Y Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, - - 2014
TRAIL is a death receptor ligand that induces cell death preferentially in tumor cells. Recombinant soluble TRAIL, however, performs poorly as an anti-cancer therapeutic because oligomerization is required for potent biological activity. We previously generated a diabody format of tumor-targeted TRAIL termed DbαEGFR-scTRAIL, comprising single-stranded TRAIL molecules (scTRAIL) and the ...
Ohinata Yasuhide Y PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan; Life Science Experimental Facility, Department of Biotechnology, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan; Laboratory for Pluripotent Stem Cell Studies, Center for Developmental Biology, RIKEN Kobe institute, Kobe Chuo-ku, Hyogo, - - 2014
The inner cell mass (ICM) and trophoblast cell lineages duet early embryonic development in mammals. After implantation, the ICM forms the embryo proper as well as some extraembryonic tissues, whereas the trophoectoderm (TE) exclusively forms the fetal portion of the placenta and the trophoblast giant cells. Although embryonic stem (ES) ...
Brooks Michael D MD Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri, United States of America; Department of Genetics and Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, Missouri, United States of - - 2014
Cell-cell interactions between tumor cells and constituents of their microenvironment are critical determinants of tumor tissue biology and therapeutic responses. Interactions between glioblastoma (GBM) cells and endothelial cells (ECs) establish a purported cancer stem cell niche. We hypothesized that genes regulated by these interactions would be important, particularly as therapeutic ...
Koehler Bruno Christian BC National Center for Tumor Diseases, Department of Medical Oncology, Internal Medicine VI, Heidelberg University Hospital, Heidelberg, - - 2014
Despite the fact that new treatment regimes have improved overall survival of patients challenged by colorectal cancer (CRC), prognosis in the metastatic situation is still restricted. The Bcl-2 family of proteins has been identified as promising anti cancer drug target. Even though small molecules targeting Bcl-2 proteins are in clinical ...
Zheng Bo'an B Department of Colorectal Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, - - 2014
Due to changes in lifestyle, particularly changes in dietary habits, colorectal cancer (CRC) increased in recent years despite advances in treatment. Nearly one million new cases diagnosed worldwide and half a million deaths make CRC a leading cause of cancer mortality. In the present study, we aimed to investigate the ...
Sarkar Purbasha P Energy Biosciences Institute, University of California, Berkeley, California, United States of America; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of - - 2014
Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The precise spatial three-dimensional (3D) organization of cellulose, hemicellulose, pectin and lignin ...
Ansari I A IA Department of Pathology, Meenakshi Medical College and Research Institute, Enathur, Kanchipuram, Tamilnadu, - - 2014
Hilus cells are ovarian counterpart of testicular leydig cells seen at hilar region of ovary. We present an interesting case of hilus cell hyperplasia in fallopian tube associated with multiple leiomyomata of uterus in a postmenopausal woman. To our knowledge very few cases reported the existence of hilus cells outside ...
Howie Duncan D Sir William Dunn School of Pathology, University of Oxford , Oxford , - - 2014
We have proposed that tolerance can be maintained through the induction, by Treg cells, of a tolerogenic microenvironment within tolerated tissues that inhibits effector cell activity but which supports the generation of further Treg cells by "infectious tolerance." Two important components of this tolerogenic microenvironment depend on metabolism and nutrient ...
Kim Hyonchol H Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, - - 2014
We propose a new method of size separation of cells exploiting precisely size-controlled hemispherical superparamagnetic microparticles. A three-layered structure of a 2-nm nickel layer inserted between 15-nm silicon dioxide layers was formed on polystyrene cast spheres by vapor deposition. The polystyrene was then removed by burning and the hemispherical superparamagnetic ...
Falck Miniotis Maria M Department of Biomedical Laboratory Science, Health and Society, Malmö University and Malmö University Hospital, Malmö, - - 2014
Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study ...
Dyshlovoy Sergey A SA 0000-0002-7155-9245 Laboratory of Marine Natural Products Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, Vladivostok 690022, Russia ; Department of Oncology, Haematology and Bone Marrow Transplantation with Section Pneumology, Hubertus Wald-Tumorzentrum, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany ; School of Natural Sciences, Far East Federal University, Sukhanova Street 8, Vladivostok 690950, - - 2014
Aaptamine (8,9-dimethoxy-1H-benzo[de][1,6]naphthyridine) is a marine natural compound possessing antioxidative, antimicrobial, antifungal, and antiretroviral activity. Earlier, we have found that aaptamine and its derivatives demonstrate equal anticancer effects against the human germ cell cancer cell lines NT2 and NT2-R and cause some changes in the proteome of these cells. In order ...
Oda Yoshihisa Y Center for Frontier Research, National Institute of Genetics Mishima, Japan ; The Graduate University For Advanced Studies Mishima, Japan ; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency Kawaguchi, - - 2014
Regulation of plant cell wall deposition and patterning is essential for the normal growth and development of plants. Small GTPases play pivotal roles in the modulation of primary cell wall formation by controlling cytoskeletal organization and membrane trafficking. However, the functions of small GTPases in secondary cell wall development are ...
Karnavas Theodoros T Chromatin Dynamics Unit, San Raffaele University and Research Institute Milan, Italy ; HMGBiotech Srl Milan, - - 2014
Mouse Embryonic Stem Cells (ESCs) are pluripotent mammalian cells derived from the Inner Cell Mass (ICM) of mouse blastocysts, which give rise to all three embryonic germ layers both in vivo and in vitro. Mouse ESCs have a distinct epigenetic landscape and a more decondensed chromatin compared to differentiated cells. ...
Hou Yue Y Department of Chemical and Biological Engineering, Iowa State University, - - 2014
Cell clustering and scattering play important roles in cancer progression and tissue engineering. While the extracellular matrix (ECM) is known to control cell clustering, much of the quantitative work has focused on the analysis of clustering between cells with strong cell-cell junctions. Much less is known about how the ECM ...
Rahman Amirah Abdul AA UKM Medical Molecular Biology Institute (UMBI), UKM Medical Center, Jalan Ya'acob Latiff, Bandar Tun Razak, Cheras 56000, Malaysia. - - 2014
Plant bioactives [6]-gingerol (GING), epigallocatechin gallate (EGCG) and asiaticoside (AS) and vitamin E, such as tocotrienol-rich fraction (TRF), have been reported to possess anticancer activity. In this study, we investigated the apoptotic properties of these bioactive compounds alone or in combination on glioma cancer cells. TRF, GING, EGCG and AS ...
Zhang Jun J State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; Institute of Life Sciences, Chongqing Medical University, Chongqing, - - 2014
We previously established and characterized two insect cell lines (BmN-SWU1 and BmN-SWU2) from Bombyx mori ovaries. Here, we examined their differential susceptibilities to Bombyx mori nucleopolyhedrovirus (BmNPV) despite having originated from the same tissue source. BmN-SWU1 cells were susceptible and supported high titers of BmNPV replication, while BmN-SWU2 cells were ...
Pende Nika N 1] Department of Ecogenomics and Systems Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria - - 2014
Two long-standing paradigms in biology are that cells belonging to the same population exhibit little deviation from their average size and that symmetric cell division is size limited. Here, ultrastructural, morphometric and immunocytochemical analyses reveal that two Gammaproteobacteria attached to the cuticle of the marine nematodes Eubostrichus fertilis and E. ...
Fukuda Kyohei K Department of Applied Biochemistry, Tokai - - 2014
This study sought to devise a way to assess the infiltration of cancer cells in model stromal tissues. Human breast carcinoma MDA-MB-231 cells were loaded on the surface of a type I collagen gel in the well of 8-well chamber slide and allowed to migrate into the gel. The gel ...
Choi Bo-Hyun BH College of pharmacy, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of - - 2014
Photodynamic therapy (PDT) has emerged as an effective treatment for various solid tumors. The transcription factor NRF2 is known to protect against oxidative and electrophilic stress; however, its constitutive activity in cancer confers resistance to anti-cancer drugs. In the present study, we investigated NRF2 signaling as a potential molecular determinant ...
Nefzger Christian M CM Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash - - 2014
Mature cells can be reprogrammed to a pluripotent state. These so called induced pluripotent stem (iPS) cells are able to give rise to all cell types of the body and consequently have vast potential for regenerative medicine applications. Traditionally iPS cells are generated by viral introduction of transcription factors Oct-4, ...
Lucas Thaís Fg TF Section of Experimental Endocrinology; Department of Pharmacology; Escola Paulista de Medicina; Universidade Federal de São Paulo; INFAR; Vila Clementino; São Paulo, SP - - 2014
The identification of the hormones and other factors regulating Sertoli cell survival, proliferation, and maturation in neonatal, peripubertal, and pubertal life remains one of the most critical questions in testicular biology. The regulation of Sertoli cell proliferation and differentiation is thought to be controlled by cell-cell junctions and a set ...
Hoelzle David J DJ Department of Integrative Biology and Physiology, University of California, Los Angeles; Department of Aerospace and Mechanical Engineering, University of Notre - - 2014
Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an efficient manner. Typically, data for ~10(2) cells can be acquired within a 1 hr experiment. An automated image analysis program enables efficient post-experiment analysis of ...
Dysart Marilyn M MM The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of - - 2014
Dysfunctional pulmonary homeostasis and repair, including diseases such as pulmonary fibrosis (PF), chronic obstructive pulmonary disease (COPD), and tumorigenesis have been increasing over the past decade, a fact that heavily implicates environmental influences. Several investigations have suggested that in response to increased transforming growth factor - beta (TGFβ) signaling, the ...
Giuliani Anna Lisa AL Department of Morphology, Surgery and Experimental Medicine, Section of Experimental Pathology, Oncology and Biology, University of Ferrara, Ferrara, - - 2014
The P2X7 receptor (P2X7R) is attracting increasing attention for its involvement in cancer. Several recent studies have shown a crucial role of P2X7R in tumour cell growth, angiogenesis and invasiveness. In this study, we investigated the role of the two known human P2X7R functional splice variants, the full length P2X7RA ...
Abd El-Rahman Atef Abd El-Mohsen AA Department of Agricultural chemistry, Faculty of Agriculture, Minia University, El-Minia, Egypt E-mail : - - 2014
Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, ...
Wang Yuzhen Y Department of Hematology, The Affiliated Hospital of Medical College Qingdao University, 16 Jiangsu Road, Qingdao, Shandong 266003, - - 2014
This study had investigated the possible toxicity of graphene oxide and its mechanisms on multiple myeloma cells (RPMI 8226 cells) using flow cytometry and a multifunctional microplate reader. RPMI 8226 cells were cultured with various concentrations of graphene oxide, then cell viability, malondialdehyde, glutathione and apoptosis were measured. We found ...
Yang Mei M Wuhan City Vocational College, 10 West Yezhi Lake Road, Mahu Village, Hongshan District, Wuhan 430064, - - 2014
To investigate the effect of downregulation of SIRT3 expression on cell proliferation and invasion in esophageal squamous cell carcinoma (ESCC) EC9706 cells, and to explore its possible molecular mechanisms, we transfected siRNA targeting SIRT3 into EC9706 cells, and then divided cells into three groups: untreated, control siRNA and SIRT3 siRNA ...
Su Wen-Ta WT Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1 Sec. 3, Chung-Hsiao E. Rd, Taipei 10608, - - 2014
Stem cells from human exfoliated deciduous teeth (SHEDs) are mesenchymal stem cells isolated from the exfoliated human deciduous incisor that can differentiate into a many cell types. In this study, we evaluated the effect of liquorice or angelica extracts on the hepatic differentiation potential of SHEDs cells. SHEDs cells cultured ...
Lin Shaogang S Hospital of Putian University, Putian 351100, Fujian Province, - - 2013
Wistar rats were intragastrically perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedii (Epimedium), Semen Cuscutae (Dodder Seed), or Herba Cistanches (Desertliving Cistanche), 0.04 mg/mL monoamine oxidase-B inhibitor selegiline, or distilled water for 14 consecutive days to prepare drug-containing serum or blank serum. ...
Jiang Hong H Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China ; Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, - - 2013
Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress co-listin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5-500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In ...
Hapieienko D D - - 2013
Under current conditions the probability of simultaneous effects of radiation and chemical factors on biological objects increases, and that's why the issue of combined influence of factore different by their nature becomes progressively important. The objective was to study the cytotoxicity of nickel ions in a test-system of cell culture ...
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