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Wilson Thomas C TC Department of Pathology, University of Iowa, 200 Hawkins Drive, Iowa City, IA, 52242, - - 2014
Basal cell adenoma and basal cell adenocarcinoma represent uncommon basaloid salivary gland neoplasms that show marked morphologic similarity. We wished to compare clinical outcome and morphologic features as well as growth and proliferation associated markers for both neoplasms. We reviewed the pathologic features of 70 neoplasms diagnosed as basal cell ...
Uchida Makoto M Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, - - 2014
Corynebacterium glutamicum is known to perform a unique form of cell division called post-fission snapping division. In order to investigate the mechanism of cell division of this bacterium, we isolated temperature-sensitive mutants from C. glutamicum wild-type strain ATCC 31831, and found that one of them, M45, produced high frequencies of ...
Gudarzi Hoda H a Department of Biology , Faculty of Science, Kharazmi University , Tehran , - - 2014
Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of ...
Singh Purva - - 2014
Mesenchymal cell condensation is the initiating event in endochondral bone formation. Cell condensation is followed by differentiation into chondrocytes accompanied by induction of chondrogenic gene expression. Gene mutations involved in chondrogenesis cause chondrodysplasias and other skeletal defects. Using mesenchymal stem cells (MSCs) in an in vitro chondrogenesis assay, we found ...
D'Souza Sonia S The Institute for Complex Engineered Systems, Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, PA 15213, - - 2014
The field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago. One remaining obstacle, especially for conjugation of polymers to cells, has been that exquisite control over polymer structure and functionality has not been used ...
Song Yongxin Y Department of Marine Engineering, Dalian Maritime University, Dalian, 116026, P. R. - - 2014
An electrokinetic microfluidic chip is developed to detect and sort target cells by size from human blood samples. Target Cell detection is achieved by a differential resistive pulse sensor (RPS) based on the size difference between the target cell and the other cells. Once a target cell is detected, the ...
LaLonde Alexandra A Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY, - - 2014
The effects of insulator-based dielectrophoresis (iDEP) manipulation on cell viability were investigated by varying operating conditions and the shape of the insulating structures. Experiments were conducted with E. coli, B. subtilis and S. cerevisiae cells by varying the applied potential (300-1000 V), exposure time (1-4 min) and composition of the ...
Tang Liying L Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei, Beijing 100700, China; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, - - 2014
Six spirostanol glycosides (1-6) and 12 known compounds (7-18) were isolated from the underground parts of Dracaena thalioides (Agavaceae). Their structures were determined by spectroscopic analysis, including 2D NMR spectroscopic data, and chemical transformations. The isolated compounds were evaluated for cytotoxic activity against HL-60 human leukemia cells. Compounds 1, 3-6, ...
Dehne Tilo T Berlin-Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Berlin, - - 2014
The negligible self-repair potential of the myocardium has led to cell-based tissue engineering approaches to restore heart function. There is more and more consensus that, in addition to cell development, paracrine effects in particular play a pivotal role in the repair of heart tissue. Here, we present two complementary murine ...
Xiang Fenfen F Department of Laboratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. - - 2014
The purpose of the present study was to investigate the relationship between myeloid differentiation primary response gene 88 (MyD88) expression and the resistance to paclitaxel of A549 lung cancer cells. In order to achieve MyD88 gene overexpression or knockdown in the A549 cell line, the cells were infected with lentivirus carrying ...
Gotoh Shimpei S Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. Electronic address: - - 2014
No methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been reported. Based on a study of the stepwise induction of alveolar epithelial cells (AECs), we identified carboxypeptidase M (CPM) as a surface marker of NKX2-1(+) "ventralized" ...
Piekarski A L AL Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville - - 2014
The major objectives of this study were to compare cell bioenergetics in 2 avian liver cell lines under control conditions and in response to oxidative stress imposed by 4-hydroxy 2-nonenal (4-HNE). Cells in this study were from a chemically immortalized Leghorn male hepatoma (LMH) cell line and a spontaneously immortalized ...
Allaman-Pillet Nathalie N IRO, Institute for Research in Ophthalmology - - 2014
Retinoblastoma is the most common pediatric intraocular neoplasm. While retinoblastoma development requires the inactivation of both alleles of the RB tumor-suppressor gene (RB1) in the developing retina, additional genomic changes are involved in tumor progression, which progressively lead to resistance of tumor cells to death. Therapeutics acting at very downstream ...
Panopoulos Andreas A *Tumor Initiation and Maintenance Program, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037 - - 2014
Tetraploidy can arise from various mitotic or cleavage defects in mammalian cells, and inheritance of multiple centrosomes induces aneuploidy when tetraploid cells continue to cycle. Arrest of the tetraploid cell cycle is therefore potentially a critical cellular control. We report here that primary rat embryo fibroblasts (REF52) and human foreskin ...
Lechuga Susana S Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA 23298, - - 2014
Trans-differentiation of epithelial cells into mesenchymal cells and myofibroblasts plays an important role in tumor progression and tissue fibrosis. Such epithelial plasticity is accompanied by dramatic reorganizations of the actin cytoskeleton, although mechanisms underlying cytoskeletal effects on epithelial trans-differentiation remain poorly understood. In the present study, we observed that selective ...
Osborne Lukas D LD *Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC, - - 2014
Recent studies implicate a role for cell mechanics in cancer progression. The epithelial to mesenchymal transition (EMT) regulates the detachment of cancer cells from the epithelium and facilitates their invasion into stromal tissue. While classic EMT hallmarks include loss of cell-cell adhesions, morphology changes, and increased invasion capacity, little is ...
Ishii Hiroki H University of Yamanashi, - - 2014
Epithelial splicing regulatory protein 1 (ESRP1) and 2 (ESRP2) regulate alternative splicing events associated with epithelial phenotypes of cells, and both are down-regulated during the epithelial-mesenchymal transition (EMT). However, little is known about their expression and functions during carcinogenesis. In this study, we found that expression of both ESRP1 and ...
Weinberg Marc S MS Gene Therapy - - 2014
Understanding the entry and trafficking mechanism(s) of rAAV into host cells can lead to evolution in capsid and vector design and delivery methods, resulting in enhanced transduction and therapeutic gene expression. Variability of findings regarding the early entry pathway of rAAV supports the possibility that rAAV, like other viruses, can ...
Cavodeassi Florencia F Centro de Biología Molecular "Severo Ochoa", - - 2014
Melanin synthesis constitutes a fundamental aspect of the terminal differentiation program of pigment cells. In vertebrates, there are two major pigment cell types: melanocytes, derived from the neural crest and cells of the pigmented layer of the retina, known as retinal pigmented epithelium (RPE), which are generated from neuroepithelial cells ...
Levin Gromiko Uliana U Department of Physiology and Cell Biology, Ben-Gurion University of the Negev, Beer-Sheva, - - 2014
Nuclear factors of activated T-cells (NFAT) are critical modulators of cancer cell growth and survival. However, the mechanisms of their oncogenic dysregulation and strategies for targeting in tumors remain elusive. Here, we report coupling of anti-apoptotic NFAT (NFAT2) activation to cholesterol-enriched lipid raft microdomains of malignant melanoma cells and interruption ...
Lima Nathália Bastos NB Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida AlbertoLamego, 2000 - Parque Califórnia, CEP: 28013-602, Campos dos Goytacazes, Rio de Janeiro, - - 2014
The seed coat develops primarily from maternal tissues and comprises multiple cell layers at maturity, providing a metabolically dynamic interface between the developing embryo and the environment during embryogenesis, dormancy and germination of seeds. Seed coat development involves dramatic cellular changes, and the aim of this research is to investigate ...
Ogura Naoki N Central Pharmaceutical Research Institute, Japan Tobacco Inc., Osaka, Japan. Electronic address: - - 2014
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) plays a central role in chronic HBV infection. However, analysis of the molecular mechanism of cccDNA formation is difficult because of the low efficiency in tissue cultured cells. In this study, we developed a more efficient cccDNA expression cell, Hep38.7-Tet, by ...
Ferrari Silvia Martina SM Department of Clinical and Experimental Medicine, University of Pisa, Pisa, - - 2014
We report the antineoplastic and anti-angiogenic activity of the pyrazolo[3,4-d]pyrimidine derivative CLM3 and the cyclic amide CLM94, both multiple tyrosine kinase inhibitors (TKIs), in human primary medullary thyroid cancer (P-MTC) cells, and in vitro in the medullary thyroid cancer (MTC) cell lines TT (harboring a RET C634W activating mutation) and MZ-CRC-1 ...
Chang Boyang B State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, 510060 Guangzhou, Guangdong, P.R. China; Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. - - 2014
Bmi-1 had been found to involve in self renewal of stem cells and tumorigenesis in various malignancies. In this study, we investigated the role of Bmi-1 in the development of salivary adenoid cystic carcinoma (SACC). At first, we confirmed that the deregulation of Bmi-1 was a frequent event in SACC; ...
Squadrito Mario Leonardo ML Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, - - 2014
MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of miRNA transfer to acceptor cells are unclear. We used macrophages and endothelial cells (ECs) as a model ...
Zhang Jin J Department of Obstetrics and Gynecology, Beijing Shijitan Hospital, Capital Medical University, Haidian, Beijing 100038, P.R. - - 2014
The objective of the present study was to investigate the association between paclitaxel resistance, gene copy number, and gene expression in ovarian carcinoma, and to restore paclitaxel sensitivity in a paclitaxel-resistant ovarian carcinoma cell line by using hMSH2-targeting siRNA. Paclitaxel-resistant ovarian carcinoma cell lines OC3/TAX300 and OC3/TAX50 and their parental ...
Cai Kemin K Department of Otorhinolaryngology Head and Neck Surgery, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. - - 2014
The anaphylatoxin C5a is a chemoattractant that can induce various inflammatory responses in vivo via the C5a receptor (C5aR). There is emerging evidence that C5a is generated in the cancer microenvironment. However, the role of C5a in human nasopharyngeal carcinoma (NPC) remains largely unclear. Thus, the present study aimed to examine the direct ...
Mishra Dhruva K DK Department of Surgery, Houston Methodist Research Institute, Houston, - - 2014
Currently, there is no in vitro or ex vivo model that can isolate circulating tumor cells (CTCs). Recently, we developed a four-dimensional (4D) lung cancer model that allows for the isolation of CTCs. We postulated that these cells have different properties than parental (2D) cells. We obtained CTCs by growing A549, H1299, ...
Moniot Brigitte B Genetic and Development department, Institute of Human Genetics, CNRS UPR1142, Montpellier 34094, Cedex 05, - - 2014
Through intercellular signalling, the somatic compartment of the foetal testis is able to program primordial germ cells to undergo spermatogenesis. Fibroblast growth factor 9 and several members of the transforming growth factor β superfamily are involved in this process in the foetal testis, counteracting the induction of meiosis by retinoic ...
Djuzenova Cholpon S CS Department of Radiation Oncology, University Hospital, Josef-Schneider-Strasse 11, D-97080 Würzburg, Germany. Electronic address: - - 2014
Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, actin cytoskeleton organization and ...
Chiang Kun-Chun KC General Surgery Department, Chang Gung Memorial Hospital, Keelung, Taiwan, - - 2014
Pancreatic cancer (PDA) is a devastating disease and there is no effective treatment available at present. To develop new regiments against PDA is urgently needed. Previously we have shown that vitamin D analog, MART-10 (19-nor-2α-(3-hydroxypropyl)-1α,25(OH)2D3), exerted potent antiproliferative effect on PDA in vitro and in vivo without causing hypercalcemia. Since ...
Ling Hui H Key Laboratory of Tumor Cellular and Molecular Pathology (University of South China), College of Hunan Province, Cancer Research Institute, Hengyang, Hunan 421001, P.R. - - 2014
Previous studies have shown that diallyl disulfide (DADS), a naturally occurring anticancer agent in garlic, arrested human gastric cancer cells (MGC803) in the G2/M phase of the cell cycle. Due to the importance of cell cycle redistribution in DADS-mediated anticarcinogenic effects, we investigated the role of checkpoint kinases (Chk1 and ...
Chen Yingyu Y Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fuzhou 350001, Fujian, P.R. - - 2014
Emodin, an extracted natural compound from the root and rhizome of Rheum palmatum L, has been shown to have multiple biological activities including anticancer functions in previous studies. In this study, we investigated the anti-leukemic activity of emodin alone or emodin in the presence all-trans retinoic acid (ATRA) in acute ...
Jackstadt Rene R Experimental and Molecular Pathology, Institute of Pathology, Ludwig-Maximilians-Universität München, Munich, - - 2014
AP4 represents a c-MYC-inducible bHLH-LZ transcription factor, which displays elevated expression in many types of tumors. We found that serum-starved AP4-deficient mouse embryo fibroblasts (MEFs) were unable to resume proliferation and showed a delayed S-phase entry after restimulation. Furthermore, they accumulated as tetraploid cells due to a cytokinesis defect. In ...
Cao Wen-Jia - - 2014
As a newly discovered tumor-associated gene, p42.3 was originally ascertained in gastric cancer cell line BGC823 and has been confirmed as a cell cycle-dependent gene that is overexpressed in many human tumor cell lines and embryonic tissues. p42.3 can regulate the level of relative cycle-dependent proteins and promote malignant transformation ...
Schell C C Renal Division, University Hospital Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), Albert Ludwigs University Freiburg, Germany; Faculty of Biology, Albert Ludwigs University - - 2014
The glomerulus represents a highly structured filtration unit, composed of glomerular endothelial cells, mesangial cells, podocytes and parietal epithelial cells. During glomerulogenesis an intricate network of signaling pathways involving transcription factors, secreted factors and cell-cell communication is required to guarantee accurate evolvement of a functional, complex 3-dimensional glomerular architecture. Here, ...
Chen Yi-Chen YC Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan, - - 2014
Caffeate derivatives have been reported to exhibit antioxidant, anti-inflammatory, and anticancer activities. To reveal the cytotoxic and apoptotic effects of caffeate derivatives, we studied the effects of octyl, phenylpropyl, and decyl caffeates on cell growth and apoptosis in A549 human lung carcinoma cells. A549 human lung carcinoma cells were treated ...
Chang Yuqiao Y Department of Human Anatomy and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, - - 2014
Fibroblasts are biologically dynamic and morphologically heterogeneous and are the most abundant connective tissue cells, with diverse structures depending on their location and activity. The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. Recent advances in our ...
Cao Hong-Yong HY Department of General Surgery, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. - - 2014
Aptamers have emerged as promising molecular probes for disease diagnosis and therapy. In the present study, the entire live cell-SELEX method was used to generate gastric cancer cell‑specific aptamers. Human gastric carcinoma AGS cells were used as target cells for positive selections and human normal gastric epithelial GES-1 cells as ...
Park Sungwoo S Global Research Laboratory; School of Biological Science/Bio-MAX Institute; Seoul National University; Seoul, - - 2014
CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and ...
van Doorn Wouter G WG Mann Laboratory, Department of Plant Sciences, University of California, Davis, CA 95616, USA. Electronic address: - - 2014
Epidermis cells in the outer tepals of Iris flowers (Iris×hollandica, cv. Blue Magic) start programmed cell death (PCD) prior to floral opening. The tepals show visible senescence symptoms three days after full opening. Visible senescence coincides with collapse (death) of the upper epidermis cells. In these cells, electron-dense particles (plastoglobuli), ...
Luo Ran R Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, - - 2014
The pathogenesis of glioma is unclear. The therapeutic results are very poor currently. This study aims to investigate the endothelial cell-derived plasminogen in the promotion of glioma cell proliferation. The results showed that after exposure to polyIC, the production of plasminogen by Huvecs was markedly increased. Plasmin cleaved PAR2 on ...
Harris Katharine G KG Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, United - - 2014
Enteroviruses (EVs) are the most common human viral pathogens. They cause a variety of pathologies, including myocarditis and meningoencephalopathies, and have been linked to the onset of type I diabetes. These pathologies result from the death of cells in the myocardium, central nervous system, and pancreas, respectively. Understanding the role ...
Rosseti Isabela Bueno IB Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba - UNIVAP, Av. Shishima Hifumi, 2911, CEP 12244-000, São José dos Campos, SP, - - 2014
The opportunistic fungal Candida albicans can produce superficial and systemic infections in immunocompromised patients. An essential stage to both colonization and virulence by C. albicans is the transition from budding yeast form to filamentous form, producing biofilms. In this work, we studied the effect of the organochalcogenide compound (PhSe)2 on ...
Hamada Taiji T Department of Molecular and Cellular Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, - - 2014
The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-positive MCF-7 and ER-negative SK-BR-3 cells expressed ...
Amrutha K K Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri Campus, Clappana P.O., Kollam 690 525, Kerala, - - 2014
Seventeen flavonoids with different substitutions were evaluated for inhibition of nuclear factor-κB (NF-κB) signaling in the invasive breast cancer cell line MDA-MB-231. They were screened using an engineered MDA-MB-231 cell line reporting NF-κB activation. The modulation of expression of two NF-κB regulated genes involved in tumorigenesis, matrix metalloproteinase-9 (MMP-9), and ...
Ashkenazi Avi A Genentech, Inc., South San Francisco, CA 94080; email: - - 2014
Cell turnover is a fundamental feature in metazoans. Cells can die passively, as a consequence of severe damage to their structural integrity, or actively, owing to a more confined biological disruption such as DNA damage. Passive cell death is uncontrolled and often harmful to the organism. In contrast, active cell ...
Wu Zheng-Rong ZR School of Pharmaceutics, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, People's Republic of - - 2014
In order to generate compounds with superior antitumor activity and reduced toxicity, twelve new hydroxycinnamic acid hydrazide derivatives were synthesized and evaluated for their antiproliferative activities against two cancer cell lines (H1299 lung carcinoma cells and MCF-7 breast cancer cells), and compared to two normal counterparts (NL-20 lung epithelial cells ...
Zhang Wenjing W MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, - - 2014
Thermo-sensitive microgel particles may exert a swelling force inside cells and influence on cell viability due to their volume transition in response to external temperature change. In this study, cross-linked poly(N-isopropylacrylamide) (PNIPAM) microgel particles with a thermo-responsive volume expansion ability were prepared by precipitation polymerization of NIPAM, poly(ethylene glycol) diacrylate ...
Jia Rui-Zhe RZ Department of Obstetrics, Nanjing Maternal and Child Health Hospital, Affiliated with Nanjing Medical University, Nanjing, - - 2014
Background/Aims: The invasion of trophoblast cells into the maternal uterine decidua is critical for normal placentation, establishment of pregnancy and maintenance of fetal growth in humans. Several growth factors and cytokines have been implicated in trophoblast invasion, but the underlying regulatory mechanisms of invasion are not fully understood. Our earlier ...
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