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Results 451 - 500 of 2026
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Zhang Huan H State Key Laboratory of Integrated Management of Pest Insects and Rodents, Chinese Academy of Sciences, Institute of Zoology, Beichenxilu 1-5, Beijing, 100101, - - 2014
The cell lines provided excellent tools to understand the mechanism of biological phenomenon at the cellular and molecular levels. The continuous development of new cell culture technology is both of interest for use in biochemical, immunology, and virological studies. The transformation of cells of the primary culture is a key ...
Prpar Mihevc Sonja S Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230, Domzale, - - 2014
The objective of this study was morphological and functional characterization of cells from the primary cell culture developed from lactating goat mammary gland, focusing on distribution of lineage-specific markers. Primary cells were grown on a thin layer of basement membrane matrix, a growth surface that resembles in vivo conditions. The ...
Hu Yuan Y Department of Clinical Pharmacology, Pharmacy Care Center, Chinese PLA General Hospital, No.28 FuXing Road, Beijing, 100853, China, - - 2014
This study was aimed to elucidate the roles of inhibition of related JAK/STAT pathways in regulating cytotoxicity induced by cisplatin in non-small-cell lung cancer (NSCLC) cell. We treated five non-small-cell lung cancer cell lines with cisplatin alone or with cisplatin and Jak2 inhibitor (ruxolitinib) and assessed cell viability, expression of ...
Zhang Y Y Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, People's Republic of - - 2014
This study provides novel evidence that sex determining region Y (SRY)-box (SOX6) and runt-related transcription factor 2 (RUNX2) play essential roles in the communication of chondrocyte and osteoblast. Our findings open a new avenue to the limited understanding of the coordination effect between chondrogenesis and osteogenesis. Sox6 and Runx2 are ...
Ohigashi Izumi I Division of Experimental Immunology, Institute for Genomic Research, University of Tokushima, Tokushima, - - 2014
Cortical thymic epithelial cells (cTECs) and medullary thymic epithelial cells (mTECs), which play essential roles in the establishment of a functionally competent and self-tolerant repertoire of T cells, are derived from common thymic epithelial progenitor cells (pTECs). Recent findings indicate that mTECs are derived from cells that express molecules that ...
Zhang Li L Department of Gynecologic Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. - - 2014
Tumor metastasis is a prominent cause of treatment failure in cervical carcinoma. Phenethyl isothiocyanate (PEITC) is an active component extracted from cruciferous plants that has exhibited anticancer activity in various types of human cancer; however, its effect on the inhibition of metastasis remains unclear. The current study aimed to explore ...
Bonzano Sara S Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123, Torino, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano, - - 2014
The olfactory bulb (OB) is the first brain region involved in the processing of olfactory information. In adult mice, the OB is highly plastic, undergoing cellular/molecular dynamic changes that are modulated by sensory experience. Odour deprivation induces down-regulation of tyrosine hydroxylase (TH) expression in OB dopaminergic interneurons located in the ...
Zhang Yi Y Department of Gastrointestinal Surgery, Shandong Tumor Hospital, Jinan, 250017, - - 2014
RLIP76, a multidomain protein which is a downstream effector of the small GTP ases RalA and RalB, is known to play a role in biological activities in a variety of malignant cancer cells. However, little study has been done on the role of RLIP76 in CRC. In this study, a ...
Jiang Li-Cheng LC Shandong Province Key Laboratory of Oral and Maxillofacial-Head and Neck Medicine (SPKLOMHNM), Zhong Yuan Academy of Biological Medicine, Liaocheng University, and Liaocheng People's Hospital, Medical School of Liaocheng, Taishan Medical University, Liaocheng, Shandong, - - 2014
There has been little research conducted regarding autophagy in oral squamous cell carcinoma (OSCC). Given the prevalence of oral cancers which are OSCC and the severe side effects of current treatments, there is a pressing need to develop effective alternative therapies. In this study, we have endeavored to explore the ...
Garcia-Quevedo L L Unitat de Biologia Cel·lular, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), - - 2014
It has been described that aneuploidies trigger cell cycle checkpoints leading to apoptosis. The aim of this study was to assess the relationship between the presence of chromosomal abnormalities and apoptosis in germ cells and in Sertoli cells. Fourteen diagnostic testicular biopsies from infertile patients were processed following a sequential ...
Chang Ching-Wen CW Institute of Oral Biology, National Yang-Ming - - 2014
Head and neck squamous cell carcinoma (HNSCC) is a lethal cancer. Emerging evidence supports cancer-initiating cells (CICs) are responsible for tumor growth, chemoresistance, etc. However, the physiological mechanisms by which to maintain the characteristics of CICs in HNSCC (HN-CICs) remain elusive. For hematopoietic stem cells, low intracellular reactive oxygen species ...
Yin Zheng Z Department of Systems Medicine and Bioengineering, Houston Methodist Research Institute, Weill Cornell Medical College, Houston, TX, - - 2014
Through statistical analysis of datasets describing single cell shape following systematic gene depletion, we have found that the morphological landscapes explored by cells are composed of a small number of attractor states. We propose that the topology of these landscapes is in large part determined by cell-intrinsic factors, such as ...
Sathyamurthy Madhavi M Department of Biological Sciences, KAIST, 335 Gwahak-ro, Yuseong-gu, Daejeon, 305-701, Republic of - - 2014
Bone morphogenetic protein-7 (BMP-7) is synthesized as a precursor that requires proteolytic cleavage of the propeptide by proprotein convertases (PCs) for its functional activity. A high-level expression of BMP-7 in CHO cells (CHO-BMP-7) resulted in secretion of a mixture of inactive precursor and active BMP-7. In an effort to achieve ...
Park Jae Woo JW Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, - - 2014
This paper describes a novel surface immobilization method for live-cell imaging of Chlamydomonas reinhardtii for continuous monitoring of lipid droplet accumulation. Microfluidics allows high-throughput manipulation and analysis of single cells in precisely controlled microenvironment. Fluorescence imaging based quantitative measurement of lipid droplet accumulation in microalgae had been difficult due to ...
Stamer W Daniel WD Departments of Ophthalmology and Biomedical Engineering, Duke University, DUMC 3802, Durham, North Carolina, NC 27710, USA. Electronic address: - - 2014
Ocular hypertension in glaucoma develops due to age-related cellular dysfunction in the conventional outflow tract, resulting in increased resistance to aqueous humor outflow. Two cell types, trabecular meshwork (TM) and Schlemm's canal (SC) endothelia, interact in the juxtacanalicular tissue (JCT) region of the conventional outflow tract to regulate outflow resistance. ...
Sangan Caroline B CB Centre for Regenerative Medicine, University of Bath, Bath, - - 2014
There is currently a shortage of organ donors available for pancreatic beta cell transplantation into diabetic patients. An alternative source of beta cells is pre-existing pancreatic cells. While we know that beta cells can arise directly from alpha cells during pancreatic regeneration we do not understand the molecular basis for ...
Theodoraki Aikaterini A Centre for Neuroendocrinology, Royal Free Campus, UCL, London, NW3 2QG. Electronic address: - - 2014
Heparan sulfate proteoglycans (HSPGs) exist in pancreatic beta cells, and HS seems to modulate important interactions in the islet microenvironment. However, the intra-islet structures of HS in health or altered glucose homeostasis are currently unknown. Here we show that distinct spatial distribution of HS motifs is present in islets in ...
Haack Timm T Max Planck Institute for Developmental Biology, Spemannstr. 35, 72074 Tübingen, - - 2014
The Drosophila heart is a linear organ formed by the movement of bilaterally specified progenitor cells to the midline and adherence of contralateral heart cells. This movement occurs through the attachment of heart cells to the overlying ectoderm which is undergoing dorsal closure. Therefore heart cells are thought to move ...
Honkisz Ewelina E Department of Animal Biotechnology, University of Agriculture, Rędzina 1B, 30-248 Cracow, - - 2014
The goal of the present study was to investigate the impact of tetrabromobisphenol A (TBBPA) on human choriocarcinoma-derived placental JEG-3 cells in vitro. We determined the effect of this compound on estradiol secretion, aromatase protein expression and activity in vitro in the JEG-3 cell line. We assessed the ability of ...
Wang Xin-Tong XT Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, - - 2014
BIIB021 is a novel, orally available inhibitor of heat shock protein 90 (Hsp90) that is currently in phase I/II clinical trials. BIIB021 induces the apoptosis of various types of tumor cells in vitro and in vivo. The aim of this study is to investigate the effect of BIIB021 on the ...
Schmidt Martin M Institute of Biochemistry II, Jena University Hospital, Friedrich Schiller University Jena, Jena, - - 2014
The tissue availability of active glucocorticoids (cortisol in humans) depends on their rate of synthesis from cholesterol, downstream metabolism, excretion and interconversion. The latter is mediated by the 11β-hydroxysteroid dehydrogenases (11βHSDs). In this review, we summarize the features of the two isoenzymes, 11βHSD1 and 11βHSD2, and current available experimental data ...
Rodin Sergey S Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institute, Stockholm, - - 2014
A robust method for culturing human pluripotent stem (hPS) cells under chemically defined and xeno-free conditions is an important tool for stem cell research and for the development of regenerative medicine. Here, we describe a protocol for monolayer culturing of Oct-4-positive hPS cells on a specific laminin-521 (LN-521) isoform, under ...
Deng Xiaojie X Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, China Central Normal University, Wuhan, 430079, People's Republic of - - 2014
A scaffold provides a framework and initial support for the cells to attach, proliferate and differentiate, and form an extracellular matrix (ECM) in tissue engineering. Here, xyloglucan (XG) was used as a new synthetic ECM for HepG2 cell attachment in alginate capsules. The effects of XG on HepG2 cells on ...
Maffei Gabriella G Department of Biochemistry and Biophysics, Second University of Naples, via De Crecchio 7, Naples, 80138, - - 2014
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia and is currently incurable. To expand the therapeutic armamentarium, we investigated antitumor activity of pyrrolo[1,2-b][1,2,5]benzothiadiazepine (PBTDs) in MEC1 cells. We found that PBTD (RS2778) treatment enhanced the activation of pro-apoptotic members such as caspase-9, 3, poly (ADP-ribose) polymerase (PARP) and ...
Duan Hong-Gang HG Department of Otolaryngology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, - - 2014
Nasal complications after radiotherapy severely affect the quality of life of nasopharyngeal carcinoma patients, and there is a compelling need to find novel therapies for nasal epithelial cell radiation damage. Therefore, we investigated the therapeutic effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) in guinea pig model of nasal ...
Tripathi Rakshamani R Cell Death and Differentiation Research, National Institute of Immunology, New Delhi, - - 2014
Emerging interest on the interrelationship between the apoptotic and autophagy pathways in the context of cancer chemotherapy is providing exciting discoveries. Complexes formed between molecules from both pathways present potential targets for chemotherapeutics design as disruption of such complexes could alter cell survival. This study demonstrates an important role of ...
Berkholz Janine J Charité - University Medicine Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, - - 2014
Skeletal and heart muscle-specific variant of the alpha subunit of nascent polypeptide complex (skNAC) is exclusively present in striated muscle cells. During skeletal muscle cell differentiation, skNAC expression is strongly induced, suggesting that the protein might be a regulator of the differentiation process. Rhabdomyosarcoma is a tumor of skeletal muscle ...
Pirat Jean-Luc - - 2014
This paper reports the design and synthesis of C-glycoside mimetics (D-glycero-D-talo- and D-glycero-D-galactopyranose analogues) subset of the recently published phostines, belonging to the [1,2]-oxaphosphinane core. Eighteen new compounds have been tested against eleven cancer cell types belonging to six categories of tumoral tissues and three different species. The hit compound ...
Soner Burak Cem BC Necmettin Erbakan University, Meram Faculty of Medicine, Department of Medical Pharmacology, 42100, Konya, - - 2014
Flavopiridol is a flavone that inhibits several cyclin‑dependent kinases and exhibits potent growth‑inhibitory activity, apoptosis and G1‑phase arrest in a number of human tumor cell lines. Flavopiridol is currently undergoing investigation in human clinical trials. The present study focused on the effect of flavopiridol in cell proliferation, cell cycle progression ...
Zou Meijuan M Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, - - 2014
Autophagy is a tightly-regulated catabolic pathway involving degradation of cellular proteins, cytoplasm and organelles. Recent evidence suggests that autophagy plays a potential role in cell death as a tumor suppressor and that its induction especially in combination with apoptosis could be beneficial. It remains unclear if all cancer cells behave ...
You Yan Y Department of Dermatology, Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. - - 2014
Increasing evidence has demonstrated that cell adhesion molecule 1/tumor suppressor in lung cancer 1 (CADM1/TSLC1) is crucially implicated in various biological processes, including proliferation, apoptosis, and invasion during tumorigenesis and development. However, the mechanism underlying its suppression of tumor growth and metastasis in melanoma remains elusive. The aim of the present study ...
Stewart Katherine K Goodman Cancer Research Centre and Department of Biochemistry, McGill University, Montreal, 1160 Pine Avenue W., Montreal, QC, Canada, H3A - - 2014
The elaboration of functional kidneys during embryonic development proceeds in a stepwise manner, starting with the formation of the embryonic pro- and mesonephros, followed by the induction and growth of the final metanephric kidney. These early stages of urinary tract development are critical for the embryo as a failure in ...
Garcia-Contreras Rene R Dental and Advanced Studies Research Center, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan sakagami@dent.meikai.ac.jp - - 2014
Background/Aim: Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles. ...
Yoon Tae Mi TM Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwanju, South - - 2014
Aim: To evaluate the expression of Livin in human laryngohypopharyngeal squamous cell carcinoma (LHSCC) and investigate whether Livin-knockdown using small interfering-RNA (siRNA) affects tumor aggressiveness in LHSCC cells. Materials and Methods: Immunohistochemistry, reverse transcription-polymerase chain reaction, western blotting, cell invasion assay, cell migration assay, and cell apoptosis assays were performed ...
Zhu Wen W Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190, China. - - 2014
Bamboo leaf shaped poly(ethylene oxide)-b-poly(ϵ-caprolactone) (PEO-b-PCL) sheets were prepared via crystallization-driven self-assembly. By selecting an appropriate mixed solvent, the polymer sheets, with a PCL single-crystal layer sandwiched by two PEO layers, were obtained efficiently. The morphology and structure of the sheets were characterized by microscopes and diffraction techniques. As a ...
Gonsalvez D G DG Department of Anatomy and Neuroscience, University of Melbourne, 3010, - - 2014
The rate of proliferation of cells depends on the proportion of cycling cells and the frequency of cell division. Here, we describe in detail, methods for quantifying the proliferative behavior of specific cell types in situ, and use the method to examine cell cycle dynamics in two neural crest derivatives ...
Rong Sing S a Global Biologics Research , Veterinary Medicine Research & Development , Kalamazoo , MI 49007 , - - 2014
Marek's Disease virus (MDV, also known as Gallid herpesvirus 2, GaHV-2, MDV-1) causes oncogenic disease in chickens producing clinical symptoms that include lymphomas, visceral tumors, nerve lesions, and immunosuppression. MDV vaccines are widely used and mostly produced using primary cells: CEF (chicken embryo fibroblast), DEF (duck embryo fibroblast), CEK (chicken ...
Leporini Lidia L a Department of Pharmacy , University G. d'Annunzio , Chieti , - - 2014
Ethanol extracts of Stachys glutinosa L. (Lamiaceae) were investigated for antioxidative properties, as well as antiproliferative action on various cell lines. The antioxidant activities were investigated by ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) assay, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, β-carotene/linoleic acid assay, scavenging of hydrogen peroxide (horseradish peroxidase test), superoxide anion scavenging, and hypochlorous ...
Kang Wonmo W Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA. - - 2014
New techniques to deliver nucleic acids and other molecules for gene editing and gene expression profiling, which can be performed with minimal perturbation to cell growth or differentiation, are essential for advancing biological research. Studying cells in their natural state, with temporal control, is particularly important for primary cells that ...
Kim Tae-Hyung - - 2014
Nanoprobe-based techniques have emerged as an efficient tool for the manipulation and analysis of single cells. Here, we report a powerful whole-electrical single cell manipulation tool that enables rapid and controllable delivery of cargo into single neural stem cells with precision monitoring of the cell penetration process using a conductive ...
Chen Shuang S Division of Hematology/Oncology, Department of Medicine, Virginia Commonwealth University and the Massey Cancer Center, Richmond, VA, United - - 2014
Bim perturbations contribute to resistance to various standard and novel agents. Here we demonstrate that Bim plays an important functional role in bortezomib (btz) resistance in multiple myeloma (MM) cells, and that targeting Bim by combining histone deacetylase inhibitors (HDACIs e.g., SBHA) with BH3-mimetics (e.g., ABT-737) overcomes btz-resistance. BH3-only protein ...
Bessonnard Sylvain S Clermont Université, Université d'Auvergne, Laboratoire GReD, Clermont-Ferrand F-63000, France Inserm, UMR1103, Clermont-Ferrand F-63001, France CNRS, UMR6293, Clermont-Ferrand F-63001, - - 2014
During blastocyst formation, inner cell mass (ICM) cells differentiate into either epiblast (Epi) or primitive endoderm (PrE) cells, labeled by Nanog and Gata6, respectively, and organized in a salt-and-pepper pattern. Previous work in the mouse has shown that, in absence of Nanog, all ICM cells adopt a PrE identity. Moreover, ...
Salameh Ahlam Ibrahim AI Case Western Reserve - - 2014
Metabolic acidosis (MAc), a decrease in extracellular pH (pHo) caused by a decrease in [HCO3 (-)]o at a fixed [CO2]o, is a common clinical condition and causes intracellular pH (pHi) to fall. Although previous work suggests that MAc-induced decreases in pHi (ΔpHi) differ among cell types, what is not clear ...
Röhrborn Diana D Paul-Langerhans-Group for Integrative Physiology, German Diabetes Center, Düsseldorf, - - 2014
Dipeptidyl peptidase 4 is an important drug target for diabetes and a novel adipokine. However, it is unknown how soluble DPP4 (sDPP4) is cleaved from the cell membrane and released into the circulation. We show here that MMP1, MMP2 and MMP14 are involved in DPP4 shedding from human vascular smooth ...
Cheng Xudong X College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu 210046, China; Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu 210028, - - 2014
The migration and invasion characteristics that are related to inflammatory response play important roles in the development of lung cancer. Astagaloside IV (AS-IV), an effective saponin component isolated from Astragali Radix, has been reported to inhibit metastasis of tumor cells. However, little is known about the underlying mechanism of AS-IV ...
Sathish Kumar B B CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Kukrail Picnic Spot Road, P.O. CIMAP, Lucknow 226015, - - 2014
2-Methoxyestradiol (2ME2) is an investigational anticancer drug. In the present study, 2-alkoxyesters/acid and 2-benzyloxy analogues of estradiol have been synthesized as analogues of 2ME2. Three of the derivatives exhibited significant anticancer activity against human breast cancer cell lines. The best analogue of the series i.e. 24 showed stabilization of tubulin ...
Fan Yue-Chao YC Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical College, 99 Huaihai Road West, Xuzhou, 221002, Jiangsu Province, People's Republic of China, - - 2014
This study was designed to explore the role of Cullin1 (Cul1) in the pathogenesis of human glioma and to investigate the role of Cul1 in the growth, migration and invasion of glioma cells. Expression of Cul1 in 191 glioma tissues, 8 normal brain tissues and 8 tumor adjacent normal brain ...
Tang Ying Y Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. - - 2014
Overexpression of ABCG2 is considered a major mechanism of cancer drug resistance. Recent studies have shown that ABCG2 can regulate the switch between symmetric and asymmetric cell division in adult stem cells; however, the relationship between ABCG2 and cell division in drug-resistant cancer cells remains to be determined. In the ...
Guo Hua H Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of - - 2014
Survivin has multiple functions in the progression of cancer. The aim of the present study was to investigate the influence of YM155, a specific survivin inhibitor, on the biological behavior of U87 glioblastoma cells. The proliferative activity and growth rate of U87 cells were determined by colony formation assay and ...
Fanos Vassilios V Department of Surgery, Neonatal Intensive Care Unit, Puericulture Institute and Neonatal Section, Policlinico Monserrato, Azienda Ospedaliera Universitaria di Cagliari, University of Cagliari, 09042, Monserrato, CA, Italy, - - 2014
The development of the mammalian kidney is a complex and in part unknown process which requires interactions between pluripotential/stem cells, undifferentiated mesenchymal cells, epithelial and mesenchymal components, eventually leading to the coordinate development of multiple different specialized epithelial, endothelial and stromal cell types within the kidney architectural complexity. We will ...
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