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Nakagawa Shinichi S RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan, - - 2015
In situ hybridization is a powerful method of examining the spatial distribution of RNA molecules at the subcellular level and serves as a basic technique in the fields of cell and developmental biology. In this technique, target RNAs are fixed in cells using formaldehyde and then hybridized to complementary probes ...
Hirose Tetsuro T Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo, 060-0815, Japan, - - 2015
A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified ...
Zhang Zhuoyuan Z State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China ; Department of Head and Neck Cancer Surgery, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to compare the method of ultracentrifugation and density gradient separation for isolating Tca8113 human tongue squamous cell carcinoma cell line-derived exosomes. The exosomes were obtained from the culture supernatant of cultured Tca8113 cells, respectively, followed by identification with transmission electron microscopy observation and ...
Yi Sha S Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, People's Republic of - - 2014
Nucleophosmin (NPM1) is a multifunctional protein that functions as a molecular chaperone, shuttling between the nucleolus and the cytoplasm. In up to one third of patients with acute myeloid leukemia, mutation of NPM1 results in the aberrant cytoplasmic accumulation of mutant protein and is thought to be responsible for leukemogenesis. ...
Kim Hyungdo - - 2014
Ternary hybrid solar cells based on zinc oxide with wide bandgap poly(3-hexylthiophene) (P3HT) and narrow bandgap poly[2,3-bis-(3-octyloxyphenyl)quinoxaline-5,8-diyl-alt-thiophene-2,5-diyl] (PTQ1) exhibit improved photovoltaic performance compared to individual binary hybrid solar cells. The increase in the photocurrent is partly due to the complementary absorption bands, which can extend the light-harvesting range from visible ...
Mun Bora B Center for Marine Natural Products and Drug Discovery, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 151-747, Republic of - - 2014
Three new sterols, 5α,8α-epidioxy-24-norcholesta-6,9(11),22-trien-3β-ol (1), 5α,8α-epidioxy-cholesta-6,9(11),24-trien-3β-ol (2), and 5α,8α-epidioxy-cholesta-6,23-dien-3β,25-diol (3), with four known sterols (4-7) were isolated from a marine sponge Monanchora sp. Their chemical structures were elucidated by extensive spectroscopic analysis. Compounds 1 and 3-7 showed moderate cytotoxicity against several human carcinoma cell lines including renal (A-498), pancreatic (PANC-1 ...
Mariani Diana D Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, - - 2014
There is substantial interest in developing alternative strategies for cancer chemotherapy aiming to increase drug specificity and prevent tumor resistance. Calorie restriction (CR) has been shown to render human cancer cells more susceptible to drugs than normal cells. Indeed deficiency of nutrient signaling proteins mimics CR, which is sufficient to ...
Wile Brian M BM 1] Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. - - 2014
Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing approaches that are limited by available surface markers or selectable metabolic characteristics, the ...
DeBoer Jason J Department of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, - - 2014
Virus infection of a cell involves the appropriation of host factors and the innate defensive response of the cell. The identification of proteins critical for virus replication may lead to the development of novel, cell-based inhibitors. In this study we mapped the changes in T-cell nuclei during human immunodeficiency virus ...
Duarte Campos Daniela F DF RWTH Aachen University, Department of Dental Materials and Biomaterial Research, Aachen, Germany ; - - 2014
The mechanical and physicochemical effects of 3D printable hydrogels on cell behaviour are paramount features to consider before manufacturing functional tissues. We hypothesise that besides good printability and cytocompatibility of a supporting hydrogel for the manufacture of individual tissues, it is equally essential to consider beforehand the desired tissue (bone, ...
Bene László L Department of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. Electronic address: - - 2014
A new method for the simultaneous detection of rotational mobility and proximity of cell surface receptors is presented based on cell-by-cell basis measurement of polarized fluorescence intensity components of the donor and acceptor of a FRET system. In addition to the FRET efficiency and the donor and acceptor concentrations, the ...
Gallo Luciana I LI *Departments of Medicine, University of Pittsburgh, Pittsburgh, PA, - - 2014
Rab11a is a key modulator of vesicular trafficking processes, but there is limited information about the guanine nucleotide-exchange factors and GTPase-activating proteins (GAPs) that regulate its GTP-GDP cycle. We observed that in the presence of Mg(2+) (2.5 mM) TBC1D9B interacted via its TBC (Tre2-Bub2-Cdc16) domain with Rab11a, Rab11b, and Rab4a ...
Huang Z Josh ZJ Cold Spring Harbor Laboratory, New York, NY 11724, USA. Electronic address: - - 2014
The mammalian neocortex gives rise to a wide range of mental activities and consists of a constellation of interconnected areas that are built from a set of basic circuit templates. Major obstacles to understanding cortical architecture include the diversity of cell types, their highly recurrent local and global connectivity, dynamic ...
Koppers-Lalic Danijela D Department of Pathology, VU University Medical Center, 1007MB Amsterdam, the Netherlands; Exosomes Research Group, VU University Medical Center, 1007MB Amsterdam, the Netherlands. Electronic address: - - 2014
Functional biomolecules, including small noncoding RNAs (ncRNAs), are released and transmitted between mammalian cells via extracellular vesicles (EVs), including endosome-derived exosomes. The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been established. We generated small RNA profiles by RNA sequencing (RNA-seq) from a panel of ...
Tanaka Nobuyuki N Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, - - 2014
Cell-sheet-engineering based regenerative medicine is successfully applied to clinical studies, though cell sheets contain uniformly distributed cells. For the further application to complex tissues/organs, cell sheets with a multi-cellular pattern were highly demanded. Micro-contact printing is a quite useful technique for patterning proteins contained in extracellular matrix (ECM). Because ECM ...
Zhang Kai K Tianjin Key Laboratory of Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; Department of Biochemistry and Molecular Biology, Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou 515041, China; State Key Laboratory of Medicinal Chemical Biology, Department of Chemistry, Nankai University, Tianjin 300071, China. Electronic address: - - 2014
Eukaryotic DNA is packaged into a chromatin with the help of four core histones (H2A, H2B, H3, and H4). Diverse histone post-translational modifications (PTMs) are hence involved in the regulation of gene transcription. However, how this regulation does work is still poorly understood and lacks details. Here we used the ...
Seong B K A BK 1] Departments of Pediatrics and Medical Biophysics, University of Toronto, Toronto, Ontario, Canada [2] Cell Biology Program, Hospital for Sick Children Research Institute, Peter Gilgan Centre for Research and Learning, Toronto, Ontario, - - 2014
Osteosarcoma (OS) is the most common malignant bone tumor and the majority of recurrences are due to metastasis. However, the molecular mechanisms that regulate OS metastatic spread are largely unknown. In this study, we report that special AT-rich-binding protein 2 (SATB2) is highly expressed in OS cells and tumors. Short ...
Ballikaya Seda S University Hospital Heidelberg, - - 2014
Activin receptor type II (ACVR2) is a member of the transforming growth factor type II receptor (TGFBR2) family and controls cell growth and differentiation thereby acting as a tumor suppressor. ACVR2 inactivation is known to drive colorectal tumorigenesis. We used an ACVR2-deficient microsatellite unstable colon cancer cell line (HCT116) to ...
Chang Ko-Tung KT National Pingtung University of Science and Technology - Department of Vehicle Engineering, 1, Shuefu Road, Neipu Pingtung 912, - - 2014
A low-cost platform is proposed for the growth and real-time monitoring of biological cells. The main components of the platform include a polymethyl methacrylate (PMMA) cell culture microchip and a multi-channel lens-free CMOS (complementary metal-oxide-semiconductor) / light-emitting diode (LED) imaging system. The PMMA microchip comprises a three-layer structure and is ...
Kondrashina Alina V AV School of Biochemistry and Cell Biology, University College Cork, Cork, - - 2014
We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed micro-cuvettes, relative OCRs measured in 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96 well plate. Using mouse embryonic fibroblasts cell line, phosphorescent intracellular O2 probe MitoXpress(®)-Intra ...
Fontaine Laetitia L Institut des Sciences de la Vie, Université catholique de Louvain, Place Croix du Sud 5, B-1348 Louvain-la-Neuve, Belgium. Electronic address: - - 2014
Natural DNA transformation is a lateral gene transfer mechanism during which bacteria take up naked DNA from their environment and stably integrate it in their genome. The proteins required for this process are conserved between species and are produced during a specific physiological state known as competence. Although natural transformation ...
Gonçalves Cristine C Centre de Biophysique Moléculaire, CNRS UPR4301, Orléans, 45071, cedex 02, - - 2014
Bioproduction of recombinant proteins (r-proteins) and recombinant lentiviral particles (r-lentiviral particles) requires robust transfections, efficient protocols easy to implement, with good reproducibility for a maximum production of proteins and lentiviral particles in a short time with low cytotoxicity. The study aims to evaluate the capacity of histidinylated lPEI (PTG1) to ...
Heasley Lydia R LR Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, - - 2014
The septins are a family of GTP-binding proteins that form cytoskeletal filaments. Septins are highly conserved and evolutionarily ancient, but absent in land plants. The synthetic plant cytokinin forchlorfenuron (FCF) was previously shown to inhibit budding yeast cell division and induce ectopic septin structures. Subsequent studies in a wide range ...
Good Miranda E ME University of Arizona, United - - 2014
Connexin (Cx) 37 suppresses vascular and cancer cell proliferation. The carboxyl terminus (CT) and a channel able to function are necessary, and neither by itself is sufficient, for Cx37 to mediate growth suppression. Cx37 supports transmembrane and intercellular signaling by forming functional hemichannels (HCs) and gap junction channels (GJCs), respectively. ...
Mufazalov Ilgiz A IA Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University of Mainz, Obere Zahlbacher Str. 67, 55131, Mainz, Germany, - - 2014
Leukocyte infiltration of the central nervous system (CNS) occurs under certain pathogenic conditions and most often results in severe disorders. Therefore, the isolation and analysis of such infiltrating cell populations is necessary for elucidating the underlying pathogenic mechanisms. Here we describe a simple and straightforward protocol for cell isolation from ...
Piacentini Mauro M Department of Biology, University of Rome 'Tor Vergata', Rome, - - 2014
Transglutaminase type II (TG2) is a pleiotropic enzyme that exhibits various activities unrelated to its originally identified functions. Apart from post-translational modifications of proteins (peculiar to the transglutaminase family enzymes), TG2 is involved in diverse biological functions, including cell death, signaling, cytoskeleton rearrangements, displaying enzymatic activities, G-protein and non-enzymatic biological ...
Negi Lalit Mohan LM Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, - - 2014
In present investigation, we developed a novel hyaluronated cationic nanostructured lipid carrier (CNLCs) which contains CPT-11/irinotecan to target CD44 biomarker which commonly overexpresses on colon cancer. The cationic core NLCs was developed by using Capmule MCM and Compritol as mix lipid phase with 150± 2.5nm particles size and 93.98±2.5% entrapment ...
Ding Guo-Bin - - 2014
Organic/inorganic nanohybrids, which integrate advantages of the biocompatibility of organic polymers and diversified functionalities of inorganic nanoparticles, have been extensively investigated in recent years. Herein, we report the construction of arginine-glycine-aspartic acid-cysteine (RGDC) tetrapeptide functionalized and 10-hydroxycamptothecin (HCPT)-encapsulated magnetic nanohybrids (RFHEMNs) for integrin αVβ3-targeted drug delivery. The obtained RFHEMNs were ...
Houben Roland R Department of Dermatology, University Hospital Würzburg, - - 2014
Merkel cell polyomavirus (MCPyV)-positive Merkel cell carcinoma (MCC) tumor cell growth is dependent on the expression of a viral Large T antigen (LT) with an intact retinoblastoma protein (RB)-binding site. This RB-binding domain in MCPyV-LT is - in contrast to other polyomavirus LTs (e.g. SV40) - embedded between two large ...
Abe Takuya T IFOM, The FIRC Institute for Molecular Oncology Foundation, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy. Electronic address: - - 2014
Transient induction or suppression of target genes is useful to study the function of toxic or essential genes in cells. Here we apply a Tet-On 3G system to DT40 lymphoma B cell lines, validating it for three different genes. Using this tool, we then show that overexpression of the chicken ...
Manshian Bella B BB MoSAIC/Biomedical NMR Unit, Department of Medicine, Catholic University of Leuven, B3000 Leuven, - - 2014
The effects of nanoparticle (NP)-related parameters on cellular interactions are currently uncertain as analysis is complicated by the combinatorial diversity arising from the array of size, shape and surface properties. Here, we present a validated multiparametric high-content imaging method, with the utility of this approach demonstrated by in-depth analysis of ...
Liu Xiaomei - - 2014
Stable human cell lines have gradually become the preferred system for large scale production of recombinant proteins for clinical applications because of their capacity of proper protein post-translational modification and low immunogenicity. However, human cell line development technologies are commonly based on random genome integration of protein expressing genes. It ...
Jain Neeraj - - 2014
BackgroundMutation in the Wiskott-Aldrich syndrome Protein (WASP) causes Wiskott-Aldrich syndrome (WAS), X-linked thrombocytopenia (XLT) and X-linked congenital neutropenia (XLN). The majority of missense mutations causing WAS and XLT are found in the WH1 (WASP Homology) domain of WASP, known to mediate interaction with WIP (WASP Interacting Protein) and CIB1 (Calcium ...
Kim Si Hyun SH Department of Chemical Engineering, Hongik University, Seoul, Korea. - - 2014
Current in vitro cell culture models do not reflect human physiology, and various efforts have been made to enhance existing models. Reconstitution of three-dimensional (3D) tissue structure has been one of the strategies, since 3D tissue structure provides essential cellular environmental cues for cell functions. Previously, we developed a novel ...
Yamakami Yoshimi Y a Graduate School of Nanobioscience , Yokohama City University , Yokohama , - - 2014
Sublethal doses of surfactants as exemplified by NP-40 clearly induce premature senescence in normal human cells. To understand molecular basis for this phenomenon, we tried to suppress it with use of various inhibitors. An inhibitor of p38 of the MAPK family almost completely suppressed growth arrest and morphological changes induced ...
Hyun Byung-Ryool BR Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 - - 2014
Dynamically regulated changes in chromatin states are vital for normal development and can produce disease when they go awry. Accordingly, much effort has been devoted to characterizing these states under normal and pathological conditions. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the most widely used method to characterize where in ...
Jo W W Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea. - - 2014
Exosomes are enclosed compartments that are released from cells and that can transport biological contents for the purpose of intercellular communications. Research into exosomes is hindered by their rarity. In this article, we introduce a device that uses centrifugal force and a filter with micro-sized pores to generate a large ...
Hornburg Daniel D Max Planck Institute of Biochemistry, - - 2014
The fatal neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) are the most common motoneuron disease and genetic cause of infant death, respectively. Various in vitro model systems have been established to investigate motoneuron disease mechanisms - in particular immortalized cell lines and primary neurons. By quantitative ...
Lee Seung-Yon SY Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of - - 2014
Metformin, a diabetes drug, has been reported to inhibit the growth of prostate cancer cells. In this study, we investigated the effect and action mechanism of metformin on the function of androgen receptor (AR), a key molecule in the proliferation of prostate cancer cells. Metformin was found to reduce androgen-dependent ...
Isozaki Yuka Y Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, - - 2014
Objective: The aim of this study was to identify alternative compounds to the tumor suppressor miR-375 using the connectivity map (CMAP) and to validate the antitumor effects of the identified drugs in esophageal squamous cell carcinoma (ESCC). Methods: Gene profiling of miR-375-treated TE2 and T.Tn cells was applied in order ...
Qiu Liping - - 2014
Cell membrane-anchored biochemical sensors that allow real-time monitoring of the interactions of cells with their microenvironment would be powerful tools for studying the mechanisms underlying various biological processes, such as cell metabolism and cell signaling. Despite the significance of these techniques, unfortunately, their development has lagged far behind due to ...
Edgar Landon J LJ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6 - - 2014
Mass cytometry (MC) offers unparalleled potential for the development of highly parameterized assays for characterization of single cells within heterogeneous populations. Current reagents compatible with MC analysis employ antibody-metal-chelating polymer conjugates to report on the presence of biomarkers. Here, we expand the utility of MC by developing the first activity-based ...
Zhang Rong-Rong RR Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR - - 2014
A new compound, N-(4-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)phenyl)-acrylamide (probe L), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting thiol from other amino acids. On being mixed with thiol in buffered DMSO:HEPES=1:1 solution at pH 7.4, the probe exhibited the blue emission at 474nm. This probe is ...
Antunes Duarte D Control Systems Technology, Department of Mechanical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands, - - 2014
The level of a given mRNA or protein exhibits significant variations from cell-to-cell across a homogeneous population of living cells. Much work has focused on understanding the different sources of noise in the gene-expression process that drive this stochastic variability in gene-expression. Recent experiments tracking growth and division of individual ...
Gbormittah Francisca O - - 2014
Clear cell renal cell carcinoma is the most prevalent of all reported kidney cancer cases and currently there are no markers for early diagnosis. This has stimulated great research interest recently because early detection of the disease can significantly improve the low survival rate. Combining the proteome, glycoproteome and N-glycome ...
Belair David G DG Department of Biomedical Engineering, University of Wisconsin, Madison, WI, - - 2014
Growth factors (GFs) are major regulatory proteins that can govern cell fate, migration, and organization. Numerous aspects of the cell milieu can modulate cell responses to GFs, and GF regulation is often achieved by the native extracellular matrix (ECM). For example, the ECM can sequester GFs and thereby control GF ...
Elhadidy Mohamed M 1 Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University , Mansoura, Egypt - - 2014
Abstract Shiga toxin-producing Escherichia coli (STEC) is defined by the ability to produce one or more types of Shiga toxins. In an attempt to better understand the mechanisms that underlie pathogenicity among STEC foodborne infection, we compared different STEC serotypes recovered from food sources (O26:H11, O103:H2, and O157:H7) for their ...
Zhang Ye Y Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125, zhangye@stanford.edu - - 2014
The major cell classes of the brain differ in their developmental processes, metabolism, signaling, and function. To better understand the functions and interactions of the cell types that comprise these classes, we acutely purified representative populations of neurons, astrocytes, oligodendrocyte precursor cells, newly formed oligodendrocytes, myelinating oligodendrocytes, microglia, endothelial cells, ...
Garcia de la Garma Jesus J Department of Abiotic Stress and Plant Pathology, CEBAS-CSIC, PO Box 164, Murcia, - - 2014
In this study, we investigated the cellular and molecular mechanisms that regulate salt acclimation. The main objective was to obtain new insights into the molecular mechanisms that control salt acclimation. Therefore, we carried out a multidisciplinary study using proteomic, transcriptomic, subcellular and physiological techniques. We obtained a Nicotiana tabacum BY-2 ...
Melzer Susanne S LIFE - Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, Germany; Department of Pediatric Cardiology, Heart Center Leipzig, University of Leipzig, Leipzig, - - 2014
Bacterial growth is often difficult to estimate beyond classical cultivation approaches. Low cell numbers, particles or coloured and dense media may disturb reliable growth assessment. Further difficulties appear when cells are attached to surfaces and detachment is incomplete. Therefore, flow cytometry was tested and used for analysis of bacterial growth ...
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