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Nakagawa Shinichi S RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan, - - 2015
In situ hybridization is a powerful method of examining the spatial distribution of RNA molecules at the subcellular level and serves as a basic technique in the fields of cell and developmental biology. In this technique, target RNAs are fixed in cells using formaldehyde and then hybridized to complementary probes ...
Hirose Tetsuro T Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo, 060-0815, Japan, - - 2015
A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified ...
Dickinson Daniel J DJ Program in Cancer Biology, Stanford University, Stanford, CA, USA, - - 2015
The discovery of polarized epithelial tissue in the social amoeba Dictyostelium discoideum establishes this classical model organism as a novel system for the study of epithelial polarity and morphogenesis. D. discoideum grows as single cells and is easily maintained in cell culture. Starvation of the cells triggers a multicellular developmental ...
Mancini Stéphane J C SJ Centre d'Immunologie de Marseille-Luminy, INSERM U1104, CNRS UMR7280, case 906, Aix-Marseille Université, UM2, 13288, Marseille cedex 09, - - 2015
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) ...
Arnoys Eric J EJ Department of Chemistry and Biochemistry, Calvin College, Grand Rapids, MI, - - 2015
A large number of observations on the nuclear versus cytoplasmic distribution of galectin-3 have been reported, correlating the presence or absence of the protein in a particular compartment of the cell to various parameters such as source of the cells under study, specific cell type, culture conditions, proliferation status of ...
Kragler Friedrich F Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Golm/Potsdam, Germany, - - 2015
Pressure microinjection can be used to introduce fluorescent dyes and labeled macromolecules into single cells. The method allows measuring transport activity of macromolecules such as proteins and RNA molecules within and between cells. Routinely, plant mesophyll cells are injected with fluorescent dextran molecules of specific sizes to measure an increase ...
Ostrup Olga O Department of Tumor Biology, Oslo University Hospital RH, Oslo, Norway, - - 2015
When a cell is reprogrammed to a new phenotype, the nucleolus undergoes more or less dramatic modulations, which can be used as a marker for the occurrence of the reprogramming. This phenomenon is most pronounced when differentiated cells are reprogrammed to totipotency when they are submitted to cloning by somatic ...
Dahl John Arne JA Institute of Clinical Medicine, Division of diagnostics and intervention, Department of Microbiology, Oslo university hospital, Rikshospitalet, NO-0027, Oslo, Norway, - - 2015
Chromatin immunoprecipitation (ChIP) is a powerful method for mapping protein-DNA interactions in vivo. Genomic localization of histone modifications, transcription factors, and other regulatory proteins can be revealed by ChIP. However, conventional ChIP protocols require the use of large numbers of cells, which prevents the application of ChIP to rare cell ...
Re Richard N RN Departments of Medicine and Physiology, Tulane University School of Medicine, New Orleans, LA, USA, - - 2015
Many extracellular signaling proteins act within their cells of synthesis and/or in target cells after internalization. This type of action is called intracrine and it plays a role in diverse biological processes. The mechanisms of intracrine intracellular action are becoming clear thanks to the application of modern techniques of molecular ...
Bidarimath Mallikarjun M Department of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON, Canada, K7L - - 2015
Laser capture microdissection (LCM) is an excellent and perhaps the only platform to isolate homogeneous cell populations from specific microscopic regions of heterogeneous tissue section, under direct microscopic visualization. The basic operations of the LCM system are based on (a) microscopic visualization of phenotypically identified cells of interest, (b) selective ...
Mariotti Francesca R FR Department of Experimental Neuroscience, IRCCS Fondazione Santa Lucia, Via Ardeatina 306, 00142, Roma, - - 2015
Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell ...
Long Kevin K EMD Millipore, 28820 Single Oak Drive, Temecula, CA, 92590, USA, - - 2015
Autophagy, a highly regulated homeostatic degradative process, allows cells to reallocate nutrients from less important to more essential processes under extreme conditions of starvation. Autophagy also prevents the buildup of damaged proteins and organelles that cause chronic tissue damage and disease. Although a topic of great interest with involvement of ...
Katholnig Karl K Institute for Medical Genetics, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, 1090, - - 2015
The isolation of nuclei from eukaryotic cells is essential for studying the composition and the dynamic changes of the nuclear proteome to gain insight into the mechanisms of gene expression and cell signalling. Primary cells are particularly challenging for standard nuclear isolation protocols due to low protein content, sample degradation, ...
Li Zhou Fang ZF Department of Biology, South University of Science and Technology of China, 1088 Xueyuan Blvd., Nanshan, Shenzhen, Guangdong, P.R. China, - - 2015
The nucleolus was one of the first subcellular organelles to be isolated from the cell. The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins. However, the ...
Kang Mijeong M Department of Chemistry, KAIST, 291 Daehak-ro (373-1 Guseong-dong), Daejeon, 305-701, - - 2015
Intracellular delivery of exogenous materials is an essential technique required for many fundamental biological researches and medical treatments. As our understanding of cell structure and function has been improved and diverse therapeutic agents with a subcellular site of action have been continuously developed, there is a demand to enhance the ...
Sebestyén Magdolna G MG Arrowhead Research Corporation, Arrowhead Madison, Inc., 465 Science Drive, Madison, WI, 53711, - - 2015
The discoveries of RNA interference (RNAi) and short interfering RNAs (siRNAs) have provided the opportunity to treat diseases in a fundamentally new way: by co-opting a natural process to inhibit gene expression at the mRNA level. Given that siRNAs must interact with the cells' natural RNAi machinery in order to ...
Thiel William H WH Department of Internal Medicine, University of Iowa, 5202 MERF, 200 Hawkins Drive, Iowa City, IA, 52242, - - 2015
After a decade of work to address cellular uptake, the principal obstacle to RNAi-based therapeutics, there is now well-deserved, renewed optimism about RNAi-based drugs. Phase I and II studies have shown safe, strong, and durable-gene knockdown (80-90 %, lasting for a month after a single injection) and/or clinical benefit in ...
Petrini Stefania S Research Laboratories, Bambino Gesu' Pediatric Hospital IRCCS, P.zza S. Onofrio 4, 00165, Rome, Italy, - - 2015
S-glutathionylation is a mechanism of signal transduction by which cells respond effectively and reversibly to redox inputs. The glutathionylation regulates most cellular pathways. It is involved in oxidative cellular response to insult by modulating the transcription factor Nrf2 and inducing the expression of antioxidant genes (ARE); it contributes to cell ...
Viotti Manuel M Genentech Incorporated, South San Francisco, CA 94080, - - 2014
Despite the importance of the gut and its accessory organs, our understanding of early endoderm development is still incomplete. Traditionally, endoderm has been difficult to study because of its small size and relative fragility. However, recent advances in live cell imaging technologies have dramatically expanded our understanding of this tissue, ...
Marconett Crystal N CN Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America ; Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America ; Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of - - 2014
Cell-type specific gene regulation is key to gaining a full understanding of how the distinct phenotypes of differentiated cells are achieved and maintained. Here we examined how changes in transcriptional activation during alveolar epithelial cell (AEC) differentiation determine phenotype. We performed transcriptomic profiling using in vitro differentiation of human and ...
Wang Qing Q State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, College of Biology, Hunan University, Changsha, 410082, - - 2014
Because cell-specific aptamers have high potential for biomedical applications, investigation of the interaction between cell and its aptamers may be of key importance for an improved understanding of biochemical processes. Herein, the interaction between human lung adenocarcinoma A549 cell and its four aptamers was explored using single-molecule force spectroscopy (SMFS). ...
Pan Ming - - 2014
This paper describes the design and synthesis of amphiphilic silica nanoparticles for the stabilization of aqueous drops in fluorinated oils for applications in droplet microfluidics. The success of droplet microfluidics has thus far relied on one type of surfactants for the stabilization of drops. Surfactants are known to have two ...
Atkuri Kondala R KR Pfizer - - 2014
Advanced single cell analysis technologies (e.g. Mass Cytometry) that help in multiplexing cellular measurements in limited volumes of primary cells are critical in bridging the discovery efforts to successful drug approval. Mass cytometry is the state-of-the-art technology in multi-parametric single-cell analysis. Mass cytometers (also known as CyTOF or Cytometry by ...
Zechel Sabrina - - 2014
BackgroundCortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventricular zone of the MGE, but whether the underlying subventricular and mantle regions of the MGE are spatially patterned has ...
Kondo Yuki Y Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan - - 2014
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular developmen in detail. Here, we establish a novel in vitro experimental system, in which vascular development is induced in ...
Wang Yurong Y Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China, - - 2014
Concerns about the risk of titanium dioxide nanoparticles (TiO2 NPs) to human health and environment are gradually increasing due to their wide range of applications. In this study, cytotoxicity, DNA damage, and apoptosis induced by TiO2 NPs (5 nm) in A549 cells were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed the ...
Vanhoutteghem Amandine A Laboratoire de physiologie cérébrale, Centre National de la Recherche Scientifique, Université Paris Descartes, UMR 8118, Paris, - - 2014
Absence of mitosis and meiosis are distinguishing properties of male germ cells during late fetal and early neonatal periods. Repressors of male germ cell meiosis have been identified, but mitotic repressors are largely unknown, and no protein repressing both meiosis and mitosis is known. We demonstrate here that the zinc-finger ...
Norouzi Mohammad M Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, - - 2014
The novel strategies of skin regenerative treatment are aimed at the development of biologically responsive scaffolds capable of delivering multiple bioactive agents and cells to the target tissues. In this study, nanofibers of poly (lactic-co-glycolic acid) (PLGA) and gelatin were electrospun and the effect of parameters viz polymer concentration, acid ...
Ge Xiaojun X Department of Medical Oncology and Laboratory of Signal Transduction and Molecular Targeted Therapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to establish, characterize and elucidate the potential mechanisms of acquired gefitinb resistance, using the A549 human lung cancer cell line. A gefitinib‑resistant A549 sub‑clone was established by exposure to escalating gefitinib concentrations over a period of 16‑24 months. Half maximal inhibitory concentration (IC50) values were ...
Sun Chen - - 2014
Quantum dots (QDs) have found a wide range of biological applications as fluorophores due to their extraordinary brightness and high photostability that are far superior to conventional organic dyes. These traits are particularly appealing for studying cell biology under cellular autofluorescence background and with long observation period. However, it remains ...
Grecco Simone Dos S SD Centro de Ciências Naturais e Humanas, Universidade Federal do ABC/SP , Brazil - - 2014
Abstract Context: Nectandra (Lauraceae) species have been used in folk medicine as an antidiarrheal, analgesic, antifungal, etc., and have many pharmacological proprieties. Objective: Investigation of the chemical composition and cytotoxicity of essential oil from Nectandra leucantha Nees & Mart. leaves. This is the first study involving N. leucantha reported in ...
Tabbaa Suzanne S Department of Bioengineering and Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, - - 2014
The major limitation of large tissue-engineered constructs used for bone regeneration is the lack of vasculature and, therefore, lack of transport of essential nutrients, chemical factors and progenitor cells. Research approaches to improve the transport properties of large scaffolds focus on using angiogenic factors and vasculogenic cells to create new ...
Bongard Dirk D Institute of Chemistry of New Materials, University of Osnabrück, Barbarastr. 7, D-49076 Osnabrück, Germany. - - 2014
The synthesis of two types of viologen dendrimers with peripheral carboxyl groups is described. Their interaction with plasmid DNA and CT-DNA and the influence of time evolution and electrolyte on dendriplex formation have been electrochemically investigated. A negative potential shift appearing in the cyclic voltammograms of the dendrimers indicates dendriplex ...
Xu Yanping Y Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA, - - 2014
A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the ...
Pegeot Mathieu M Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France CNRS, IBS, F-38027 Grenoble, France CEA, DSV, IBS, F-38027 Grenoble, - - 2014
Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding depends on the level of HS sulfation and epimerization which are cell specific and dynamically regulated. Characterization of this molecule, however, has been restricted to oligosaccharide fragments available in large amount for ...
Schwanbeck Ralf R Institute of Biochemistry, Medical Faculty, University of Kiel, Olshausenstr. 40, 24098, Kiel, - - 2014
The Notch pathway is a highly conserved cell-cell communication pathway in metazoan involved in numerous processes during embryogenesis, development, and adult organisms. Ligand-receptor interaction of Notch components on adjacent cells facilitates controlled sequential proteolytic cleavage resulting in the nuclear translocation of the intracellular domain of Notch (NICD). There it binds ...
Panikkanvalappil Sajanlal R - - 2014
Owing to the dynamic and complex nature of mitosis, precise and timely executions of biomolecular events are critical for high fidelity cell division. In this context, visualization of such complex events at the molecular level can provide vital information on the biomolecular processes in abnormal cells. Here, we explored the ...
Jung Yong-Gyun YG Quantamatrix Inc, 104-213, Seoul National University, Seoul 151-742, Republic of - - 2014
A variety of systems have been developed to study biofilm formation. However, most of the systems are based on the surface-attached growth of microbes under shear stress. In this study, we designed a microfluidic channel device, called an MAC (microfluidic agarose channel), and found that microbial cells in the MAC ...
Shannahan Jonathan H JH * Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045, - - 2014
Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming ...
Madeo Marianna M Sanford Research, United - - 2014
SV2A is a synaptic vesicle membrane protein expressed in neurons and endocrine cells and involved in the regulation of neurotransmitter release. Although the exact function of SV2A still remains elusive, it was identified as the specific binding site for levetiracetam, a second generation antiepileptic drug. Our sequence analysis demonstrates that ...
Chen Jin J Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. - - 2014
The aim of the present study was to evaluate the association between DNA methylation and multidrug resistance (MDR) in glioma and identify novel effectors responsible for MDR in human gliomas. An MDR glioma cell line, SGH‑44/ADM, was developed using adriamycin (ADM) impulse treatment. Cryopreservation, recovery and withdrawal were performed to ...
Li Yin Y Laboratory of Reproductive and Developmental Toxicology - - 2014
Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines in circulation, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. ...
Sang Xiao-Jing XJ Key Laboratory of Polyoxometalate Science of Ministry of Education, Department of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China. wangeb889@nenu.edu.cn - - 2014
Two novel open-chain carboxyethyltin decorated sandwich-type germanotungstates have been successfully synthesized. They could markedly increase the electrocatalytic activity of single-walled carbon nanotubes toward triiodide reduction when assembled into composite electrodes, which have shown a conversion efficiency of 6.32% that is comparable to that of Pt electrodes (6.29%) when used as ...
Berger Gregory G Department of Infectious Diseases, Faculty of Life Sciences and Medicine, King's College London, Guy's Hospital, London SE1 - - 2014
The accessory gene vpr, common to all primate lentiviruses, induces a potent G2/M arrest in cycling cells. A recent study showed that HIV-1 Vpr mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi Anemia DNA repair pathway. To confirm these observations, we have examined ...
Ortogero Nicole N University of Nevada, Reno, United - - 2014
PIWI-interacting RNAs (piRNAs) are small noncoding RNAs that bind PIWI family proteins exclusively expressed in the germ cells of mammalian gonads. MIWI2-associated piRNAs are essential for silencing transposons during primordial germ cell development, and MIWI-bound piRNAs are required for normal spermatogenesis during adulthood in mice. Although piRNAs have long been ...
Hwang Kevin K Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 - - 2014
DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, ...
Xu Xun X Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195, Berlin, - - 2014
Directing stem cells towards a desired location and function by utilizing the structural cues of biomaterials is a promising approach for inducing effective tissue regeneration. Here, the cellular response of human adipose-derived mesenchymal stem cells (hADSCs) to structural signals from microstructured substrates comprising arrays of square-shaped or round-shaped microwells is ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, Council of Science and Industrial Research, Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007 (India); Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad-500 037 (India). - - 2014
A series of chalcone conjugates featuring the imidazo[2,1-b]thiazole scaffold was designed, synthesized, and evaluated for their cytotoxic activity against five human cancer cell lines (MCF-7, A549, HeLa, DU-145 and HT-29). These new hybrid molecules have shown promising cytotoxic activity with IC50 values ranging from 0.64 to 30.9 μM. Among them, (E)-3-(6-(4-fluorophenyl)-2,3-bis(4-methoxyphenyl)imidazo[2,1-b]thiazol-5-yl)-1-(pyridin-2-yl)prop-2-en-1-one ...
Zhang Shaolian S Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. typh@jnu.edu.cn - - 2014
A highly sensitive and recyclable quartz crystal microbalance (QCM) biosensor was developed using chitosan (CS) and folic acid (FA), generating conjugates that are selectively recognized by MCF-7 cancer cell over-expressed folic acid receptors. The prepared CS-FA conjugate was characterized by UV-vis spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy ...
Gallagher Kimberly L KL Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104; email: gallagkl@sas.upenn.edu , - - 2014
Development in multicellular organisms requires the coordinated production of a large number of specialized cell types through sophisticated signaling mechanisms. Non-cell-autonomous signals are one of the key mechanisms by which organisms coordinate development. In plants, intercellular movement of transcription factors and other mobile signals, such as hormones and peptides, is ...
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