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Zhang Zhuoyuan Z State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China ; Department of Head and Neck Cancer Surgery, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to compare the method of ultracentrifugation and density gradient separation for isolating Tca8113 human tongue squamous cell carcinoma cell line-derived exosomes. The exosomes were obtained from the culture supernatant of cultured Tca8113 cells, respectively, followed by identification with transmission electron microscopy observation and ...
Mun Bora B Center for Marine Natural Products and Drug Discovery, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 151-747, Republic of - - 2014
Three new sterols, 5α,8α-epidioxy-24-norcholesta-6,9(11),22-trien-3β-ol (1), 5α,8α-epidioxy-cholesta-6,9(11),24-trien-3β-ol (2), and 5α,8α-epidioxy-cholesta-6,23-dien-3β,25-diol (3), with four known sterols (4-7) were isolated from a marine sponge Monanchora sp. Their chemical structures were elucidated by extensive spectroscopic analysis. Compounds 1 and 3-7 showed moderate cytotoxicity against several human carcinoma cell lines including renal (A-498), pancreatic (PANC-1 ...
Wile Brian M BM 1] Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. - - 2014
Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing approaches that are limited by available surface markers or selectable metabolic characteristics, the ...
Gallo Luciana I LI *Departments of Medicine, University of Pittsburgh, Pittsburgh, PA, - - 2014
Rab11a is a key modulator of vesicular trafficking processes, but there is limited information about the guanine nucleotide-exchange factors and GTPase-activating proteins (GAPs) that regulate its GTP-GDP cycle. We observed that in the presence of Mg(2+) (2.5 mM) TBC1D9B interacted via its TBC (Tre2-Bub2-Cdc16) domain with Rab11a, Rab11b, and Rab4a ...
Huang Z Josh ZJ Cold Spring Harbor Laboratory, New York, NY 11724, USA. Electronic address: - - 2014
The mammalian neocortex gives rise to a wide range of mental activities and consists of a constellation of interconnected areas that are built from a set of basic circuit templates. Major obstacles to understanding cortical architecture include the diversity of cell types, their highly recurrent local and global connectivity, dynamic ...
Seong B K A BK 1] Departments of Pediatrics and Medical Biophysics, University of Toronto, Toronto, Ontario, Canada [2] Cell Biology Program, Hospital for Sick Children Research Institute, Peter Gilgan Centre for Research and Learning, Toronto, Ontario, - - 2014
Osteosarcoma (OS) is the most common malignant bone tumor and the majority of recurrences are due to metastasis. However, the molecular mechanisms that regulate OS metastatic spread are largely unknown. In this study, we report that special AT-rich-binding protein 2 (SATB2) is highly expressed in OS cells and tumors. Short ...
Ballikaya Seda S University Hospital Heidelberg, - - 2014
Activin receptor type II (ACVR2) is a member of the transforming growth factor type II receptor (TGFBR2) family and controls cell growth and differentiation thereby acting as a tumor suppressor. ACVR2 inactivation is known to drive colorectal tumorigenesis. We used an ACVR2-deficient microsatellite unstable colon cancer cell line (HCT116) to ...
Chang Ko-Tung KT National Pingtung University of Science and Technology - Department of Vehicle Engineering, 1, Shuefu Road, Neipu Pingtung 912, - - 2014
A low-cost platform is proposed for the growth and real-time monitoring of biological cells. The main components of the platform include a polymethyl methacrylate (PMMA) cell culture microchip and a multi-channel lens-free CMOS (complementary metal-oxide-semiconductor) / light-emitting diode (LED) imaging system. The PMMA microchip comprises a three-layer structure and is ...
Kondrashina Alina V AV School of Biochemistry and Cell Biology, University College Cork, Cork, - - 2014
We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed micro-cuvettes, relative OCRs measured in 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96 well plate. Using mouse embryonic fibroblasts cell line, phosphorescent intracellular O2 probe MitoXpress(®)-Intra ...
Gonçalves Cristine C Centre de Biophysique Moléculaire, CNRS UPR4301, Orléans, 45071, cedex 02, - - 2014
Bioproduction of recombinant proteins (r-proteins) and recombinant lentiviral particles (r-lentiviral particles) requires robust transfections, efficient protocols easy to implement, with good reproducibility for a maximum production of proteins and lentiviral particles in a short time with low cytotoxicity. The study aims to evaluate the capacity of histidinylated lPEI (PTG1) to ...
Heasley Lydia R LR Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, - - 2014
The septins are a family of GTP-binding proteins that form cytoskeletal filaments. Septins are highly conserved and evolutionarily ancient, but absent in land plants. The synthetic plant cytokinin forchlorfenuron (FCF) was previously shown to inhibit budding yeast cell division and induce ectopic septin structures. Subsequent studies in a wide range ...
Good Miranda E ME University of Arizona, United - - 2014
Connexin (Cx) 37 suppresses vascular and cancer cell proliferation. The carboxyl terminus (CT) and a channel able to function are necessary, and neither by itself is sufficient, for Cx37 to mediate growth suppression. Cx37 supports transmembrane and intercellular signaling by forming functional hemichannels (HCs) and gap junction channels (GJCs), respectively. ...
Mufazalov Ilgiz A IA Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University of Mainz, Obere Zahlbacher Str. 67, 55131, Mainz, Germany, - - 2014
Leukocyte infiltration of the central nervous system (CNS) occurs under certain pathogenic conditions and most often results in severe disorders. Therefore, the isolation and analysis of such infiltrating cell populations is necessary for elucidating the underlying pathogenic mechanisms. Here we describe a simple and straightforward protocol for cell isolation from ...
Piacentini Mauro M Department of Biology, University of Rome 'Tor Vergata', Rome, - - 2014
Transglutaminase type II (TG2) is a pleiotropic enzyme that exhibits various activities unrelated to its originally identified functions. Apart from post-translational modifications of proteins (peculiar to the transglutaminase family enzymes), TG2 is involved in diverse biological functions, including cell death, signaling, cytoskeleton rearrangements, displaying enzymatic activities, G-protein and non-enzymatic biological ...
Negi Lalit Mohan LM Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, - - 2014
In present investigation, we developed a novel hyaluronated cationic nanostructured lipid carrier (CNLCs) which contains CPT-11/irinotecan to target CD44 biomarker which commonly overexpresses on colon cancer. The cationic core NLCs was developed by using Capmule MCM and Compritol as mix lipid phase with 150± 2.5nm particles size and 93.98±2.5% entrapment ...
Liu Xiaomei - - 2014
Stable human cell lines have gradually become the preferred system for large scale production of recombinant proteins for clinical applications because of their capacity of proper protein post-translational modification and low immunogenicity. However, human cell line development technologies are commonly based on random genome integration of protein expressing genes. It ...
Ding Guo-Bin - - 2014
Organic/inorganic nanohybrids, which integrate advantages of the biocompatibility of organic polymers and diversified functionalities of inorganic nanoparticles, have been extensively investigated in recent years. Herein, we report the construction of arginine-glycine-aspartic acid-cysteine (RGDC) tetrapeptide functionalized and 10-hydroxycamptothecin (HCPT)-encapsulated magnetic nanohybrids (RFHEMNs) for integrin αVβ3-targeted drug delivery. The obtained RFHEMNs were ...
Houben Roland R Department of Dermatology, University Hospital Würzburg, - - 2014
Merkel cell polyomavirus (MCPyV)-positive Merkel cell carcinoma (MCC) tumor cell growth is dependent on the expression of a viral Large T antigen (LT) with an intact retinoblastoma protein (RB)-binding site. This RB-binding domain in MCPyV-LT is - in contrast to other polyomavirus LTs (e.g. SV40) - embedded between two large ...
Abe Takuya T IFOM, The FIRC Institute for Molecular Oncology Foundation, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy. Electronic address: - - 2014
Transient induction or suppression of target genes is useful to study the function of toxic or essential genes in cells. Here we apply a Tet-On 3G system to DT40 lymphoma B cell lines, validating it for three different genes. Using this tool, we then show that overexpression of the chicken ...
Manshian Bella B BB MoSAIC/Biomedical NMR Unit, Department of Medicine, Catholic University of Leuven, B3000 Leuven, - - 2014
The effects of nanoparticle (NP)-related parameters on cellular interactions are currently uncertain as analysis is complicated by the combinatorial diversity arising from the array of size, shape and surface properties. Here, we present a validated multiparametric high-content imaging method, with the utility of this approach demonstrated by in-depth analysis of ...
Jain Neeraj - - 2014
BackgroundMutation in the Wiskott-Aldrich syndrome Protein (WASP) causes Wiskott-Aldrich syndrome (WAS), X-linked thrombocytopenia (XLT) and X-linked congenital neutropenia (XLN). The majority of missense mutations causing WAS and XLT are found in the WH1 (WASP Homology) domain of WASP, known to mediate interaction with WIP (WASP Interacting Protein) and CIB1 (Calcium ...
Kim Si Hyun SH Department of Chemical Engineering, Hongik University, Seoul, Korea. - - 2014
Current in vitro cell culture models do not reflect human physiology, and various efforts have been made to enhance existing models. Reconstitution of three-dimensional (3D) tissue structure has been one of the strategies, since 3D tissue structure provides essential cellular environmental cues for cell functions. Previously, we developed a novel ...
Yamakami Yoshimi Y a Graduate School of Nanobioscience , Yokohama City University , Yokohama , - - 2014
Sublethal doses of surfactants as exemplified by NP-40 clearly induce premature senescence in normal human cells. To understand molecular basis for this phenomenon, we tried to suppress it with use of various inhibitors. An inhibitor of p38 of the MAPK family almost completely suppressed growth arrest and morphological changes induced ...
Hyun Byung-Ryool BR Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 - - 2014
Dynamically regulated changes in chromatin states are vital for normal development and can produce disease when they go awry. Accordingly, much effort has been devoted to characterizing these states under normal and pathological conditions. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the most widely used method to characterize where in ...
Jo W W Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea. - - 2014
Exosomes are enclosed compartments that are released from cells and that can transport biological contents for the purpose of intercellular communications. Research into exosomes is hindered by their rarity. In this article, we introduce a device that uses centrifugal force and a filter with micro-sized pores to generate a large ...
Hornburg Daniel D Max Planck Institute of Biochemistry, - - 2014
The fatal neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) are the most common motoneuron disease and genetic cause of infant death, respectively. Various in vitro model systems have been established to investigate motoneuron disease mechanisms - in particular immortalized cell lines and primary neurons. By quantitative ...
Lee Seung-Yon SY Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of - - 2014
Metformin, a diabetes drug, has been reported to inhibit the growth of prostate cancer cells. In this study, we investigated the effect and action mechanism of metformin on the function of androgen receptor (AR), a key molecule in the proliferation of prostate cancer cells. Metformin was found to reduce androgen-dependent ...
Isozaki Yuka Y Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, - - 2014
Objective: The aim of this study was to identify alternative compounds to the tumor suppressor miR-375 using the connectivity map (CMAP) and to validate the antitumor effects of the identified drugs in esophageal squamous cell carcinoma (ESCC). Methods: Gene profiling of miR-375-treated TE2 and T.Tn cells was applied in order ...
Qiu Liping - - 2014
Cell membrane-anchored biochemical sensors that allow real-time monitoring of the interactions of cells with their microenvironment would be powerful tools for studying the mechanisms underlying various biological processes, such as cell metabolism and cell signaling. Despite the significance of these techniques, unfortunately, their development has lagged far behind due to ...
Edgar Landon J LJ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6 - - 2014
Mass cytometry (MC) offers unparalleled potential for the development of highly parameterized assays for characterization of single cells within heterogeneous populations. Current reagents compatible with MC analysis employ antibody-metal-chelating polymer conjugates to report on the presence of biomarkers. Here, we expand the utility of MC by developing the first activity-based ...
Antunes Duarte D Control Systems Technology, Department of Mechanical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands, - - 2014
The level of a given mRNA or protein exhibits significant variations from cell-to-cell across a homogeneous population of living cells. Much work has focused on understanding the different sources of noise in the gene-expression process that drive this stochastic variability in gene-expression. Recent experiments tracking growth and division of individual ...
Gbormittah Francisca O - - 2014
Clear cell renal cell carcinoma is the most prevalent of all reported kidney cancer cases and currently there are no markers for early diagnosis. This has stimulated great research interest recently because early detection of the disease can significantly improve the low survival rate. Combining the proteome, glycoproteome and N-glycome ...
Belair David G DG Department of Biomedical Engineering, University of Wisconsin, Madison, WI, - - 2014
Growth factors (GFs) are major regulatory proteins that can govern cell fate, migration, and organization. Numerous aspects of the cell milieu can modulate cell responses to GFs, and GF regulation is often achieved by the native extracellular matrix (ECM). For example, the ECM can sequester GFs and thereby control GF ...
Elhadidy Mohamed M 1 Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University , Mansoura, Egypt - - 2014
Abstract Shiga toxin-producing Escherichia coli (STEC) is defined by the ability to produce one or more types of Shiga toxins. In an attempt to better understand the mechanisms that underlie pathogenicity among STEC foodborne infection, we compared different STEC serotypes recovered from food sources (O26:H11, O103:H2, and O157:H7) for their ...
Zhang Ye Y Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125, zhangye@stanford.edu - - 2014
The major cell classes of the brain differ in their developmental processes, metabolism, signaling, and function. To better understand the functions and interactions of the cell types that comprise these classes, we acutely purified representative populations of neurons, astrocytes, oligodendrocyte precursor cells, newly formed oligodendrocytes, myelinating oligodendrocytes, microglia, endothelial cells, ...
Garcia de la Garma Jesus J Department of Abiotic Stress and Plant Pathology, CEBAS-CSIC, PO Box 164, Murcia, - - 2014
In this study, we investigated the cellular and molecular mechanisms that regulate salt acclimation. The main objective was to obtain new insights into the molecular mechanisms that control salt acclimation. Therefore, we carried out a multidisciplinary study using proteomic, transcriptomic, subcellular and physiological techniques. We obtained a Nicotiana tabacum BY-2 ...
Melzer Susanne S LIFE - Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, Germany; Department of Pediatric Cardiology, Heart Center Leipzig, University of Leipzig, Leipzig, - - 2014
Bacterial growth is often difficult to estimate beyond classical cultivation approaches. Low cell numbers, particles or coloured and dense media may disturb reliable growth assessment. Further difficulties appear when cells are attached to surfaces and detachment is incomplete. Therefore, flow cytometry was tested and used for analysis of bacterial growth ...
Hung Joy M JM School of Chemical Sciences, The University of Auckland, Auckland, New - - 2014
Forty seven thieno[2,3-b]pyridines-2-carboxamides, furo[2,3-b]pyridines-2-carboxamides and tetrahydrothieno[2,3-b]quinolones-2-carboxamides derivatives were synthesized and tested for their antiproliferative activity against the NCI-60 cell lines. The 5-keto-tetrahydrothieno[2,3-b]quinolones-2-carboxamides (series 17) were found to have the greatest activity, with the compound containing a 3-methoxyphenylcarboxamide (compound 17d) being the most active, with GI50 values in the low nanomolar range ...
Bugay Aleksandr N AN Laboratory of Radiation Biology, Joint Institute for Nuclear Research, 6 Joliot-Curie Street, 141980 Dubna, Moscow Region, Russia. Electronic address: - - 2014
A model of the UV-induced mutation process in Escherichia coli bacteria has been developed taking into account the whole sequence of molecular events starting from initial photo-damage and finishing with the fixation of point mutations. The wild-type phenotype bacterial cells are compared with UV-sensitive repair-deficient mutant cells. Attention is mainly ...
Möll Andrea A Division of Infectious Diseases, Brigham and Women's Hospital, Boston, MA, USA. Department of Microbiology and Immunobiology, Harvard Medical School and HHMI, Boston, MA, - - 2014
Synthesis and hydrolysis of septal peptidoglycan (PG) are critical processes at the conclusion of cell division that enable separation of daughter cells. Cleavage of septal PG is mediated by PG amidases, hydrolytic enzymes that release peptide side chains from the glycan strand. Most γ-proteobacteria, including Escherichia coli, encode several functionally ...
Kumar Ashok A AA School of Engineering and Applied - - 2014
Although effective low-cost interventions exist, child mortality attributable to sickle cell disease (SCD) remains high in low-resource areas due, in large part, to the lack of accessible diagnostic methods. The presence of dense (ρ > 1.120 g/cm(3)) cells is characteristic of SCD. The fluid, self-assembling step-gradients in density created by ...
Gürtler A A Federal Office for Radiation Protection, Department SG Radiation Protection and Health , Neuherberg , Germany - - 2014
Abstract Context: Radio-sensitivity in normal tissue is characterized by heterogeneity throughout the population and the absence of pre-diagnostic biomarkers. Objective: We conducted a proteomic approach to search for radiation characteristic protein regulation. Materials and methods: Cell lines were 10 Gy irradiated and analysed by 2D-DIGE after 24 h. Results were analysed intra- ...
Löfgren Maria M Section of Pathology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, - - 2014
Formation of synovial joints includes phenotypic changes of the chondrocytes and the organisation of their extracellular matrix is regulated by different factors and signalling pathways. Increased knowledge of the normal processes involved in joint development may be used to identify similar regulatory mechanisms during pathological conditions in the joint. Samples ...
Hua R R Dept of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, S119228; Dept of Obstetrics & Gynaecology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, - - 2014
To detect aneuploidy from single fetal nucleated red blood cells (FNRBCs) from placental villi using whole genome amplification (WGA) and next generation sequencing. Three single FNRBCs per sample were manually picked from villi collected from ten women undergoing elective first trimester termination of pregnancy and one or two cells were ...
Chen Chang-Wen CW Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu, 30013, - - 2014
A novel sequential layer-by-layer sub-100 °C vacuum-sublimation method to fabricate planar-type organometal halide perovskite solar cells is developed. Very uniform and highly crystalline perovskite thin films with 100% surface coverage are produced. The cells attain maximum and average efficiencies up to 15.4% and 14%, respectively. This low- temperature, all-vacuum process ...
Tung Nguyen Huu NH Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki 859-3298, - - 2014
The crude extract of Alnus japonica bark exhibited a strong effect on the growth of Trypanosoma brucei. Subsequent chromatographic separation resulted in the isolation of two novel diarylheptanoids, known as alnuside C (2) and alnuside D (3), and three known compounds, 1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)-heptan-3(R)-O-β-D-glucopyranoside (1), oregonin (4) and hirsutanone (5). The structures ...
Mi Hao-Yang HY The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715; Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, - - 2014
Soft and hard thermoplastic polyurethane (TPU) and their blends were electrospun to fabricate nanofibrous scaffolds with various properties in order to investigate the substrate property effects on cellular response. The scaffolds were characterized with Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, water contact angle tests, and protein absorption ...
Park Ha-Young HY a Department of Bioscience and Biotechnology , Graduate School of Kyushu University , Fukuoka - - 2014
We investigated the effect of theaflavins (TFs) on membrane barrier of Caco-2 cells. For fluorescein-transport experiments, the apparent permeability (Papp) of fluorescein in Caco-2 cells pretreated with 20 μM TFs were significantly decreased compared with that in untreated cells. Although the respective monomeric catechins did not show any Papp reduction, purpurogallin ...
Toyjanova Jennet J School of Engineering, Brown University, 182 Hope St. Box D, Providence, RI, USA. - - 2014
Native cell-material interactions occur on materials differing in their structural composition, chemistry, and physical compliance. While the last two decades have shown the importance of traction forces during cell-material interactions, they have been almost exclusively presented on purely elastic in vitro materials. Yet, most bodily tissue materials exhibit some level ...
Chapot-Chartier Marie-Pierre - - 2014
The cell wall of Gram-positive bacteria is a complex assemblage of glycopolymers and proteins. It consists of a thick peptidoglycan sacculus that surrounds the cytoplasmic membrane and that is decorated with teichoic acids, polysaccharides, and proteins. It plays a major role in bacterial physiology since it maintains cell shape and ...
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