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Nakagawa Shinichi S RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan, - - 2015
In situ hybridization is a powerful method of examining the spatial distribution of RNA molecules at the subcellular level and serves as a basic technique in the fields of cell and developmental biology. In this technique, target RNAs are fixed in cells using formaldehyde and then hybridized to complementary probes ...
Hirose Tetsuro T Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo, 060-0815, Japan, - - 2015
A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified ...
Dickinson Daniel J DJ Program in Cancer Biology, Stanford University, Stanford, CA, USA, - - 2015
The discovery of polarized epithelial tissue in the social amoeba Dictyostelium discoideum establishes this classical model organism as a novel system for the study of epithelial polarity and morphogenesis. D. discoideum grows as single cells and is easily maintained in cell culture. Starvation of the cells triggers a multicellular developmental ...
Mancini Stéphane J C SJ Centre d'Immunologie de Marseille-Luminy, INSERM U1104, CNRS UMR7280, case 906, Aix-Marseille Université, UM2, 13288, Marseille cedex 09, - - 2015
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) ...
Arnoys Eric J EJ Department of Chemistry and Biochemistry, Calvin College, Grand Rapids, MI, - - 2015
A large number of observations on the nuclear versus cytoplasmic distribution of galectin-3 have been reported, correlating the presence or absence of the protein in a particular compartment of the cell to various parameters such as source of the cells under study, specific cell type, culture conditions, proliferation status of ...
Kragler Friedrich F Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Golm/Potsdam, Germany, - - 2015
Pressure microinjection can be used to introduce fluorescent dyes and labeled macromolecules into single cells. The method allows measuring transport activity of macromolecules such as proteins and RNA molecules within and between cells. Routinely, plant mesophyll cells are injected with fluorescent dextran molecules of specific sizes to measure an increase ...
Ostrup Olga O Department of Tumor Biology, Oslo University Hospital RH, Oslo, Norway, - - 2015
When a cell is reprogrammed to a new phenotype, the nucleolus undergoes more or less dramatic modulations, which can be used as a marker for the occurrence of the reprogramming. This phenomenon is most pronounced when differentiated cells are reprogrammed to totipotency when they are submitted to cloning by somatic ...
Dahl John Arne JA Institute of Clinical Medicine, Division of diagnostics and intervention, Department of Microbiology, Oslo university hospital, Rikshospitalet, NO-0027, Oslo, Norway, - - 2015
Chromatin immunoprecipitation (ChIP) is a powerful method for mapping protein-DNA interactions in vivo. Genomic localization of histone modifications, transcription factors, and other regulatory proteins can be revealed by ChIP. However, conventional ChIP protocols require the use of large numbers of cells, which prevents the application of ChIP to rare cell ...
Re Richard N RN Departments of Medicine and Physiology, Tulane University School of Medicine, New Orleans, LA, USA, - - 2015
Many extracellular signaling proteins act within their cells of synthesis and/or in target cells after internalization. This type of action is called intracrine and it plays a role in diverse biological processes. The mechanisms of intracrine intracellular action are becoming clear thanks to the application of modern techniques of molecular ...
Bidarimath Mallikarjun M Department of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON, Canada, K7L - - 2015
Laser capture microdissection (LCM) is an excellent and perhaps the only platform to isolate homogeneous cell populations from specific microscopic regions of heterogeneous tissue section, under direct microscopic visualization. The basic operations of the LCM system are based on (a) microscopic visualization of phenotypically identified cells of interest, (b) selective ...
Mariotti Francesca R FR Department of Experimental Neuroscience, IRCCS Fondazione Santa Lucia, Via Ardeatina 306, 00142, Roma, - - 2015
Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell ...
Long Kevin K EMD Millipore, 28820 Single Oak Drive, Temecula, CA, 92590, USA, - - 2015
Autophagy, a highly regulated homeostatic degradative process, allows cells to reallocate nutrients from less important to more essential processes under extreme conditions of starvation. Autophagy also prevents the buildup of damaged proteins and organelles that cause chronic tissue damage and disease. Although a topic of great interest with involvement of ...
Katholnig Karl K Institute for Medical Genetics, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, 1090, - - 2015
The isolation of nuclei from eukaryotic cells is essential for studying the composition and the dynamic changes of the nuclear proteome to gain insight into the mechanisms of gene expression and cell signalling. Primary cells are particularly challenging for standard nuclear isolation protocols due to low protein content, sample degradation, ...
Li Zhou Fang ZF Department of Biology, South University of Science and Technology of China, 1088 Xueyuan Blvd., Nanshan, Shenzhen, Guangdong, P.R. China, - - 2015
The nucleolus was one of the first subcellular organelles to be isolated from the cell. The advent of modern proteomic techniques has resulted in the identification of thousands of proteins in this organelle, and live cell imaging technology has allowed the study of the dynamics of these proteins. However, the ...
Kang Mijeong M Department of Chemistry, KAIST, 291 Daehak-ro (373-1 Guseong-dong), Daejeon, 305-701, - - 2015
Intracellular delivery of exogenous materials is an essential technique required for many fundamental biological researches and medical treatments. As our understanding of cell structure and function has been improved and diverse therapeutic agents with a subcellular site of action have been continuously developed, there is a demand to enhance the ...
Sebestyén Magdolna G MG Arrowhead Research Corporation, Arrowhead Madison, Inc., 465 Science Drive, Madison, WI, 53711, - - 2015
The discoveries of RNA interference (RNAi) and short interfering RNAs (siRNAs) have provided the opportunity to treat diseases in a fundamentally new way: by co-opting a natural process to inhibit gene expression at the mRNA level. Given that siRNAs must interact with the cells' natural RNAi machinery in order to ...
Thiel William H WH Department of Internal Medicine, University of Iowa, 5202 MERF, 200 Hawkins Drive, Iowa City, IA, 52242, - - 2015
After a decade of work to address cellular uptake, the principal obstacle to RNAi-based therapeutics, there is now well-deserved, renewed optimism about RNAi-based drugs. Phase I and II studies have shown safe, strong, and durable-gene knockdown (80-90 %, lasting for a month after a single injection) and/or clinical benefit in ...
Petrini Stefania S Research Laboratories, Bambino Gesu' Pediatric Hospital IRCCS, P.zza S. Onofrio 4, 00165, Rome, Italy, - - 2015
S-glutathionylation is a mechanism of signal transduction by which cells respond effectively and reversibly to redox inputs. The glutathionylation regulates most cellular pathways. It is involved in oxidative cellular response to insult by modulating the transcription factor Nrf2 and inducing the expression of antioxidant genes (ARE); it contributes to cell ...
Wang Qing Q State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, College of Biology, Hunan University, Changsha, 410082, - - 2014
Because cell-specific aptamers have high potential for biomedical applications, investigation of the interaction between cell and its aptamers may be of key importance for an improved understanding of biochemical processes. Herein, the interaction between human lung adenocarcinoma A549 cell and its four aptamers was explored using single-molecule force spectroscopy (SMFS). ...
Panikkanvalappil Sajanlal R - - 2014
Owing to the dynamic and complex nature of mitosis, precise and timely executions of biomolecular events are critical for high fidelity cell division. In this context, visualization of such complex events at the molecular level can provide vital information on the biomolecular processes in abnormal cells. Here, we explored the ...
Jung Yong-Gyun YG Quantamatrix Inc, 104-213, Seoul National University, Seoul 151-742, Republic of - - 2014
A variety of systems have been developed to study biofilm formation. However, most of the systems are based on the surface-attached growth of microbes under shear stress. In this study, we designed a microfluidic channel device, called an MAC (microfluidic agarose channel), and found that microbial cells in the MAC ...
Shannahan Jonathan H JH * Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045, - - 2014
Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming ...
Madeo Marianna M Sanford Research, United - - 2014
SV2A is a synaptic vesicle membrane protein expressed in neurons and endocrine cells and involved in the regulation of neurotransmitter release. Although the exact function of SV2A still remains elusive, it was identified as the specific binding site for levetiracetam, a second generation antiepileptic drug. Our sequence analysis demonstrates that ...
Li Yin Y Laboratory of Reproductive and Developmental Toxicology - - 2014
Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines in circulation, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. ...
Sang Xiao-Jing XJ Key Laboratory of Polyoxometalate Science of Ministry of Education, Department of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China. wangeb889@nenu.edu.cn - - 2014
Two novel open-chain carboxyethyltin decorated sandwich-type germanotungstates have been successfully synthesized. They could markedly increase the electrocatalytic activity of single-walled carbon nanotubes toward triiodide reduction when assembled into composite electrodes, which have shown a conversion efficiency of 6.32% that is comparable to that of Pt electrodes (6.29%) when used as ...
Berger Gregory G Department of Infectious Diseases, Faculty of Life Sciences and Medicine, King's College London, Guy's Hospital, London SE1 - - 2014
The accessory gene vpr, common to all primate lentiviruses, induces a potent G2/M arrest in cycling cells. A recent study showed that HIV-1 Vpr mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi Anemia DNA repair pathway. To confirm these observations, we have examined ...
Ortogero Nicole N University of Nevada, Reno, United - - 2014
PIWI-interacting RNAs (piRNAs) are small noncoding RNAs that bind PIWI family proteins exclusively expressed in the germ cells of mammalian gonads. MIWI2-associated piRNAs are essential for silencing transposons during primordial germ cell development, and MIWI-bound piRNAs are required for normal spermatogenesis during adulthood in mice. Although piRNAs have long been ...
Hwang Kevin K Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 - - 2014
DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, ...
Xu Xun X Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195, Berlin, - - 2014
Directing stem cells towards a desired location and function by utilizing the structural cues of biomaterials is a promising approach for inducing effective tissue regeneration. Here, the cellular response of human adipose-derived mesenchymal stem cells (hADSCs) to structural signals from microstructured substrates comprising arrays of square-shaped or round-shaped microwells is ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, Council of Science and Industrial Research, Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007 (India); Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad-500 037 (India). - - 2014
A series of chalcone conjugates featuring the imidazo[2,1-b]thiazole scaffold was designed, synthesized, and evaluated for their cytotoxic activity against five human cancer cell lines (MCF-7, A549, HeLa, DU-145 and HT-29). These new hybrid molecules have shown promising cytotoxic activity with IC50 values ranging from 0.64 to 30.9 μM. Among them, (E)-3-(6-(4-fluorophenyl)-2,3-bis(4-methoxyphenyl)imidazo[2,1-b]thiazol-5-yl)-1-(pyridin-2-yl)prop-2-en-1-one ...
Zhang Shaolian S Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. typh@jnu.edu.cn - - 2014
A highly sensitive and recyclable quartz crystal microbalance (QCM) biosensor was developed using chitosan (CS) and folic acid (FA), generating conjugates that are selectively recognized by MCF-7 cancer cell over-expressed folic acid receptors. The prepared CS-FA conjugate was characterized by UV-vis spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy ...
Gallagher Kimberly L KL Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104; email: gallagkl@sas.upenn.edu , - - 2014
Development in multicellular organisms requires the coordinated production of a large number of specialized cell types through sophisticated signaling mechanisms. Non-cell-autonomous signals are one of the key mechanisms by which organisms coordinate development. In plants, intercellular movement of transcription factors and other mobile signals, such as hormones and peptides, is ...
Becker Yvonne Y Massey University, Institute of Fundamental Sciences, Palmerston North, New Zealand ; - - 2014
Epichloё festucae is a mutualistic symbiont that systemically colonises the intercellular spaces of Lolium perenne leaves to form a highly structured and interconnected hyphal network. In an Agrobacterium tumefaciens T-DNA forward genetic screen we identified a mutant TM1066 that had a severe host interaction phenotype, causing stunting and premature senescence ...
Havrylov Serhiy S Goodman Cancer Research Centre, McGill University, H3A1A3, Montreal, QC, Canada; Department of Medicine, McGill University, H3A1A3, Montreal, QC, - - 2014
Acquired capacity of cancer cells to penetrate through the extracellular matrix (ECM) of surrounding tissues is a prerequisite for tumour metastatic spread - the main source of cancer-associated mortality. Through combined efforts of many research groups we are beginning to understand that the ability of cells to invade through the ...
Waki Michihiko M Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, - - 2014
Lipids comprise the primary component of cell membranes. Imaging mass spectrometry is increasingly being used to visualize membranous lipids in clinical specimens, and it has revealed that abnormal lipid metabolism is related to the development of diseases. To characterize cell populations which are rare and sparsely localized in tissues, we ...
Belyaev Victor V - - 2014
The phase retardation difference, ΔΦ, is calculated for hybrid liquid crystal (LC) cells as a function of LC pretilt angles, θ0(1), θ0(2), on the opposite substrates of the cell for the case of an arbitrary angle of light incidence in the range from 0 to 90°. An LC director configuration ...
Goswami Dibakar - - 2014
Iron overload causes progressive and sometimes irreversible damages due to accelerated production of reactive oxygen species. Desferrioxamine (DFO), a siderophore, has been used clinically to remove excess iron. However, the applications of DFO are limited because of its inability to access intracellular pools of labile iron. Cell penetrating peptides (CPPs) ...
Schomaker Markus M Biomedical Optics Department, Laser Zentrum Hannover e. V., Hollerithallee 8, 30419, Hannover, Germany. - - 2014
Gold nanoparticle mediated (GNOME) laser transfection is a powerful technique to deliver small biologically relevant molecules into cells. However, the transfection of larger and especially negatively charged DNA remains challenging. The efficiency for pDNA was 0.57% using parameter that does not influence the endo- and exogenous DNA. In order to ...
Occhetta Paola P Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, - - 2014
The ability to replicate in vitro the native extracellular matrix (ECM) features and to control the three-dimensional (3D) cell organization plays a fundamental role in obtaining functional engineered bio-constructs. In tissue engineering (TE) applications, hydrogels have been successfully implied as biomatrices for 3D cell embedding, exhibiting high similarities to the ...
Gough Mallory M Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YQ, U.K.. Electronic address: - - 2014
Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of ...
Oh Seung Soo SS Materials Department, ‡Department of Chemistry and Biochemistry, §Department of Mechanical Engineering, ∥Materials Research Laboratory, University of California , Santa Barbara, California 93106, United - - 2014
Viruses have evolved specialized mechanisms to efficiently transport nucleic acids and other biomolecules into specific host cells. They achieve this by performing a coordinated series of complex functions, resulting in delivery that is far more efficient than existing synthetic delivery mechanisms. Inspired by these natural systems, we describe a process ...
Fontana Gianluca - - 2014
Intradiscal injection of growth factors or cells has been shown to attenuate symptoms of intervertebral disc degeneration. However, different approaches are needed to overcome limitations such as short-term efficacy and leakage of the injected solutions. The current study aims at creating a platform for the realization of functional cell factories ...
Kawikova Ivana I Section of Clinical Immunology, Yale University School of Medicine, TAC S-217, 300 Cedar St., New Haven, CT 06511, USA. Electronic address: - - 2014
A newly discovered cell-to-cell communication system involves small, membrane-enveloped nanovesicles, called exosomes. We describe here how these extracellular nanoparticles were discovered and how it became gradually apparent that they play fundamental roles in regulation of physiological functions and pathological processes. Exosomes enable intercellular communication by transporting genetic material, proteins and ...
Safarik Ivo I Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Ceske Budejovice, Czech - - 2014
Magnetically modified yeast cells represent an interesting type of biocomposite materials, applicable in various areas of bioanalysis, biotechnology and environmental technology. In this review, typical examples of magnetic modifications of yeast cells of the Saccharomyces, Kluyveromyces, Rhodotorula and Yarrowia genera are presented, as well as their possible applications as biocatalysts, ...
Simasi Jacinta J Fraunhofer-Institut für Zelltherapie und Immunologie, Leipzig, Germany; Institut für Pharmazie, Universität Leipzig, Leipzig, Germany. Electronic address: - - 2014
Tyrosine kinase inhibitors (TKI), erlotinib and gefitinib are small molecule inhibitors which are used for the treatment of lung cancer. But, the development of drug resistance has been reported as one of the major setbacks in oncology. This study focused on the mechanisms leading to secondary resistance by assessing the ...
Balaish Moran M The Nancy & Stephen Grand Technion Energy Program, Technion-Israel Institute of Technology, Haifa, 3200003 - - 2014
Non-aqueous lithium-oxygen batteries are considered as most advanced power sources, albeit they are facing numerous challenges concerning almost each cell component. Herein, we diverge from the conventional and traditional liquid-based non-aqueous Li-O2 batteries to a Li-O2 system based on a solid polymer electrolyte (SPE-) and operated at a temperature higher ...
Lyu Aifeng A Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research and School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China; Jiangsu Hansoh Pharmceutical Corporation, Lianyungang 222000, - - 2014
A series of Lapatinib derivatives were designed and prepared by changing the straight alkyl side chain of Lapatinib into a branched one. ELISA assay and western blot analysis showed that these derivatives can significantly inhibit HER1/HER2 as well as their downstream signal transduction proteins. In vitro cytotoxicity assay revealed that these ...
Guo Xiaoling X Department of Chemistry, Harbin Institute of Technology, Harbin, 150001, China; College of Pharmacy, Jiamusi University, Jiamusi, 154000, - - 2014
Developing a reliable and cost-effective miniaturized electroanalysis tool is of vital importance for cell electrochemical analysis. In this work, a novel mini-electrochemical system has been constructed for trace detection of cell samples. The mini-electrochemical system was constructed by integrating a pencil graphite modified by threonine (PT/PGE) as working electrode, an ...
Gurunathan Sangiliyandi - - 2014
BackgroundGraphene is the 2D form of carbon that exists as a single layer of atoms arranged in a honeycomb lattice and has attracted great interest in the last decade in view of its physical, chemical, electrical, elastic, thermal, and biocompatible properties. The objective of this study was to synthesize an ...
Chatron-Colliet Aurore A UMR CNRS 7369 MEDyC, SFR Cap-Santé, URCA, Reims, - - 2014
During melanoma tumour growth, cancerous cells are exposed to the immediate surrounding the micro- and macro environment, which is largely modified through the degradation of the extracellular matrix by fibroblast-derived metalloproteinases. Among the degradation products, (VGVAPG)3, an elastin peptide is known to stimulate the proliferation of both fibroblasts and cancerous ...
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