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Results 251 - 300 of 1844
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Zhang Rong - - 2011
Purpose:  To examine the effect of intraocularly produced glucagon-like peptide-1 (GLP-1) on the survival rate of retinal ganglion cells in an optic nerve crush model. Methods:  Forty-one Sprague-Dawley rats were divided into a study group (21 animals) in which 4 beads with 3000 genetically modified cells to produce GLP-1 were ...
Villa-Forte Alexandra - - 2011
Giant cell arteritis is the most common form of vasculitis affecting older people, and the diagnosis should be considered in older patients who present with the new onset of headache, visual dysfunction, polymyalgia rheumatica, or systemic inflammatory symptoms. Physicians should be familiar with its variety of clinical presentations and the ...
Hiebl B - - 2011
Soft hydrophobic poly(n-butyl acrylate) networks (cPnBA) were developed as entropy elastic substrates for passive mechanical stimulation of cells, where the elastic modulus of the cPnBAs could be systematically adjusted by variation of the cross-link density. The networks were synthesized by thermally-induced radical polymerization from n-butyl acrylate, with poly(propylene glycol) dimethacrylate ...
Li Chen - - 2011
OBJECTIVE: This study was performed to determine whether T(H)17 cells are involved in the development and metastasis of head and neck squamous cell carcinomas (HNSCCs). STUDY DESIGN: T(H)17 cells frequencies in 67 HNSCC patients and 21 healthy volunteers were examined by flow cytometric analysis. T(H)17 cell-related cytokines in serum (interleukin ...
Shiga Tania M - - 2011
BACKGROUND: Fruit softening is generally attributed to cell wall degradation in the majority of fruits. However, unripe bananas contain a large amount of starch, and different banana cultivars vary in the amount of starch remaining in ripe fruits. Since studies on changes in pulp firmness carried out with bananas are ...
Gutschi T - - 2011
The objective of the present retrospective study was to report our experience concerning the effects of cell phone usage on semen parameters. We examined 2110 men attending our infertility clinic from 1993 to October 2007. Semen analysis was performed in all patients. Serum free testosterone (T), follicle stimulating hormone (FSH), ...
Srivastava Girish K - - 2011
The aim of this study is to investigate the use of elastin-like recombinamers (ELRs) as a substrate that can maintain the growth, phenotype, and functional characteristics of retinal pigment epithelial (RPE) cells efficiently and as a suitable carrier for the transplantation of autologous RPE cells for treatment of age-related macular ...
Sanchez-Siles M - - 2011
BACKGROUND: The most important complication of oral lichen planus is malignancy transformation. OBJECTIVE: The aim of this study was to assess cellular and nuclear morphology in a group of patients with oral lichen planus measured by means of buccal micronucleus cytome assay. STUDY DESIGN: This study included thirty patients with ...
Guo Feng - - 2011
To investigate the inducements of increase of cell hydrophobicity from aerobic biofloc (ABF) and granular sludge (AGS), in this study, as the first time the hydrophilic and hydrophobic bacterial communities were analyzed independently. Meanwhile, the effect of extracellular polymers (EPS) on the cell hydrophobicity is also studied. Few Bacteroidetes were ...
Kim Seong-Gon - - 2011
Resistance to chemotherapy is very important in the prognosis of tumors. Transglutaminase-2 (TG-2) mediated chemotherapy resistance has been widely reported. The objective of this study was to demonstrate the effect of 4-hexylresorcinol (4-HR) on TG-2 activity in nasopharyngeal squamous cell carcinoma cells (KB cells). Treatment with a mixture of 4-HR ...
González-Morán María Genoveva - - 2011
The aim of this study was to evaluate the sequence of the histological and stereological changes that occur in diverse components of left growing and right regressing ovaries of Gallus domesticus as well as the variations in the number and size of the different cell subpopulations from 8-day-old chicken embryo ...
Jorge Carla D - - 2011
Protein misfolding and deposition in the brain are implicated in the etiology of numerous neurodegenerative disorders. Here, organic solutes characteristic of microorganisms adapted to hot environments, were tested on experimental cell models of Huntington's and Parkinson's diseases. Diglycerol phosphate, di-myo-inositol phosphate, mannosylglycerate, and mannosylglyceramide were not toxic to the cells, ...
Wang Yi-Cheng - - 2011
Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. We systematically investigated how GNMT regulates methyl group kinetics and global DNA methylation. HepG2 cells (GNMT inactive, GNMT-) and cells transfected with GNMT expressed vector (GNMT+) were cultured in low (10 μmol/L), adequate (100 μmol/L), ...
Reynolds John R - - 2011
The effect of the macromolecular additive, polydimethylsiloxane (PDMS), on the performance of solution processed molecular bulk heterojunction solar cells is investigated, and the addition of PDMS is shown to improve device power conversion efficiency by ~70% and significantly reduce cell-to-cell variation, from a power conversion efficiency of 1.25 ± 0.37% ...
Rao S Madusudan - - 2011
Fluorosis is one of the manifestations of chronic poisoning from long-term exposure to high levels of fluoride. An estimated 62 million people in 17 states in India are affected with dental and skeletal fluorosis. To evaluate the cytological morphology of exfoliated oral mucosal cells among various stages of fluorosis patients ...
Cohen Arava Shatil - - 2011
The hydraulic conductivity of the leaf vascular system (K(leaf) ) is dynamic and decreases rapidly under drought stress, possibly in response to the stress phytohormone ABA which increases sharply in the xylem sap (ABA(xyl) ) during periods of drought. Vascular bundle-sheath cells (BSCs; a layer of parenchymatous cells tightly enwrapping ...
Duan Xuewu X Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China. Electronic address: - - 2011
The effects of reactive oxygen species (ROS), especially hydroxyl radical (OH), on cellular wall disassembly in vitro and in vivo, and aril breakdown in longan fruit, were investigated. OH caused the disassembly of cell wall material (CWM) in vitro, demonstrated by the increases of total sugars and uronic acid released, ...
Aly Hamdy A A - - 2011
TCDD, as an endocrine disruptor, is known to impair testicular functions and fertility. To elucidate the mechanism(s) underlying the testicular effects of TCDD, the potential toxicity of TCDD on Sertoli cells was investigated. Furthermore, the study aims to delineate whether mitochondrial fractions of Sertoli cells are involved in meditating the ...
Pulsfort Ann K - - 2011
Standard protocols for autologous fat transfer involve centrifugation of the lipoaspirate for better separation. The effect of the centrifugal forces on cell viability and possible induction of apoptosis is discussed controversially in current literature. We have examined the vitality of adipocytes in lipoaspirates after centrifugation by 8 different accelerations up ...
Louman-Gardiner K M - - 2011
Lower back pain due to intervertebral disc (IVD) degeneration is a prevalent problem which drastically affects the quality of life of millions of sufferers. Healthy IVDs begin with high populations of notochordal cells in the nucleus pulposus, while by the second stage of degeneration, these cells will be replaced by ...
Stroka Kimberly M - - 2011
Biological processes such as atherogenesis, wound healing, cancer cell metastasis, and immune cell transmigration rely on a delicate balance between Cell-Cell and cell-substrate adhesion. Cell mechanics have been shown to depend on substrate factors such as stiffness and ligand presentation, while the effects of Cell-Cell interactions on the mechanical properties ...
Wilmer Martijn J - - 2011
Recent evidence implies that impaired metabolism of glutathione has a role in the pathogenesis of nephropathic cystinosis. This recessive inherited disorder is characterized by lysosomal cystine accumulation and results in renal Fanconi syndrome progressing to end stage renal disease in the majority of patients. The most common treatment involves intracellular ...
Petrunkina A M - - 2011
Many research projects in cell biology now use flow cytometry for analysis or for isolation of specific cell types. In such studies, cell viability is obviously a crucial issue. However, many studies appear to rely upon light-scattering characteristics to identify and gate out non-viable cells, despite the fact that reliable ...
Terao Kyohei - - 2011
We propose a novel method of cell positioning using electroosmotic flow (EOF) to analyze cell-cell interactions. The EOF chip has an open-to-air configuration, is equipped with four electrodes to induce multi-directional EOF, and allows access of tools for liquid handling and of physical probes for cell measurements. Evaluation of the ...
Hégaret Hélène - - 2011
Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog ...
Reeves R Keith RK Division of Immunology, New England Primate Research Center, Harvard Medical School, Southborough Campus, Pine Hill Drive, Southborough, MA 01772, United - - 2011
Since the vast majority of infections occur at mucosal surfaces, accurate characterization of mucosal immune cells is critically important for understanding transmission and control of infectious diseases. Standard flow cytometric analysis of cells obtained from mucosal tissues can provide valuable information on the phenotype of mucosal leukocytes and their relative ...
Yáñez M Adela - - 2011
One of the greatest challenges of implementing fast molecular detection methods as part of Legionella surveillance systems is to limit detection to live cells. In this work, a protocol for sample treatment with propidium monoazide (PMA) in combination with quantitative PCR (qPCR) has been optimized and validated for L. pneumophila ...
Jacobs Tom F - - 2011
PURPOSE: More and more orthopedic procedures are performed in an outpatient setting. A commonly used strategy in pain management is the intra-articular injection of local anesthetics. Recent attention has been drawn to their possible toxic effect on chondrocytes. Local anesthetics, and in particular Lidocaine, are also used for diagnostic joint ...
DuBois Steven G - - 2012
Circulating endothelial cells (CECs) have been detected at increased numbers in patients with solid cancers. CECs have not been systematically evaluated in patients with osteosarcoma. Patients 12 months to 30 years of age with newly diagnosed high-grade osteosarcoma were eligible for this prospective cohort study. Patients provided a single blood ...
McNeely Kelsey - - 2011
The nifJ gene codes for pyruvate:ferredoxin oxidoreductase (PFOR), which reduces ferredoxin during fermentative catabolism of pyruvate to acetyl-coenzyme A (acetyl-CoA). A nifJ knockout mutant was constructed that lacks one of two pathways for the oxidation of pyruvate in the cyanobacterium Synechococcus sp. strain PCC 7002. Remarkably, the photoautotrophic growth rate ...
Dhawan Alok - - 2011
The concern over the adverse effects of nanomaterials on humans has lead to an extensive research on the fate of nanoparticles in biological systems. However, unique properties of engineered nanomaterials yield many technical challenges which impede nanotoxicity studies. Some of these challenges are: interference of nanoparticles with in vitro toxicity ...
Anderson K M - - 2011
It has been reported that human prostate-derived PC-3 cells that are CD44- and CD 41 (a2 B1)-positive are enriched in cancer stem cells. This study compared the effect of PC-3 cell proliferation under normoxia or hypoxia on the initial and subsequent expression of this doubly-labeled side-fraction. Despite the numerical advantage ...
Moura Erly Catarina - - 2011
The study objective was to evaluate the feasibility of interviews by cell phone as a complement to interviews by landline to estimate risk and protection factors for chronic non-communicable diseases. Adult cell phone users were evaluated by random digit dialing. Questions asked were: age, sex, education, race, marital status, ownership ...
Leers M P G - - 2011
Two mid-range haematology analysers (Abbott CELL-DYN Ruby and Sysmex XT-2000i) were evaluated to determine their analytical performance and workflow efficiency in the haematology laboratory. In total 418 samples were processed for determining equivalence of complete blood count (CBC) measurements, and 100 for reticulocyte comparison. Blood smears served for assessing the ...
Brooks J Renée - - 2011
• Carbon sequestration has focused renewed interest in understanding how forest management affects forest carbon gain over timescales of decades, and yet details of the physiological mechanisms over decades are often lacking for understanding long-term growth responses to management. • Here, we examined tree-ring growth patterns and stable isotopes of ...
Lee Hyoun Wook - - 2011
Renal tumors consist of heterogeneous groups that frequently show complex and overlapping morphology, thus making it difficult to make a correct diagnosis. One of the most problematic differential diagnoses is to distinguish chromophobe renal cell carcinoma (RCC) from oncocytoma. These should be distinguished by differences in their behavior and clinical ...
Jang Yun-Ho YH Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, - - 2011
In this paper, we describe a microfluidic mechanism that combines microfluidic valves and deep wells for cell localization and storage. Cells are first introduced into the device via externally controlled flow. Activating on-chip valves was used to interrupt the flow and to sediment the cells floating above the wells. Thus, ...
Taylor William W Division of Mathematical Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW71AA, UK. - - 2011
A mechanical model of cell motion was developed that reproduced the behaviour of cells in 2-dimensional culture. Cell adhesion was modelled with inter-cellular cross-links that attached for different times giving a range of adhesion strength. Simulations revealed an adhesion threshold below which cell motion was almost unaffected and above which ...
Vijendravarma R K - - 2011
Both development and evolution under chronic malnutrition lead to reduced adult size in Drosophila. We studied the contribution of changes in size vs. number of epidermal cells to plastic and evolutionary reduction of wing size in response to poor larval food. We used flies from six populations selected for tolerance ...
Serpooshan Vahid - - 2011
Scaffold microstructure is hypothesized to influence physical and mechanical properties of collagen gels, as well as cell function within the matrix. Plastic compression under increasing load was conducted to produce scaffolds with increasing collagen fibrillar densities ranging from 0.3 to above 4.1 wt % with corresponding hydraulic permeability (k) values ...
Chen Hui - - 2011
Glycerol-3-phosphate dehydrogenase (G3pdh) is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate. In this study, the effects of salinity changes on variation of cell shape and single cell glycerol content of Dunaliella salina were observed, and the effects of salinity changes on ...
Lam Yuen T - - 2011
Increased reactive oxygen species (ROS) are a feature of aging cells, but little is known about when ROS generation begins as cells age. Here we show how ROS change in Saccharomyces cerevisiae cells throughout their early replicative lifespan using the fluorescent ROS indicator, dihydroethidium (DHE), which has some specificity for ...
Muthukumar Kannan - - 2011
The present study was undertaken to determine the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells. Wild-type (WT) cells showed an overall increase in phospholipids upon 50 μM cadmium (Cd)-treatment, whereas an untreated gpx3Δ strain showed a drastic reduction in overall phospholipids which was further reduced with 50 μM Cd. ...
Poomsawat Sopee - - 2011
J Oral Pathol Med (2011) 40: 294-299 Background:  Cell cycle arrest and increased cell proliferation have been demonstrated in oral lichen planus (OLP). This study evaluated the expression of cdk4, cdk6 and p16, important proteins in the G1 phase, in OLP and compared the expression of these proteins of OLP ...
Abdulahad W H - - 2011
OBJECTIVES:: To assess the phenotype of reconstituting B-cells after B-cell depletion, and to assess the effect of reconstitution on the balance of regulatory (T(Reg)) and effector (T(eff)) T-cells in patients with active primary Sjögren's syndrome (pSS). METHODS:: Twenty-eight patients with pSS were treated on days 1 and 15 with either ...
Rusha Laert L Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St., Worcester, MA 01605, - - 2011
Three esterase-labile, but chemically-stable sulfonate protecting groups have been designed and synthesized. One of these sulfonate esters allowed the cytoplasmic delivery and unmasking of a sulfonated dye in live cells.
Wierzba-Bobrowicz Teresa - - 2011
Three calcium-binding proteins (CaBPs), calbindin D28k, calretinin and parvalbumin, were immunohistochemically examined in the cerebellum of ten-day-old rat pups of ethanol-treated dams. Dams were treated with ethanol during pregnancy and/or lactation. In the cerebellar cortex of the pups from control groups, Purkinje cells with their processes and Golgi cells were ...
Sitton Greg - - 2011
We have developed an instrument based on a flow cytometer platform that is capable of tracking individual, suspended cells over extended time periods. The instrument repeatedly moves in a capillary the same volume segment of fluid containing tens to hundreds of suspended cells through the focal point of a laser. ...
Aydin Mehmet Dumlu - - 2011
The aim of this study was to investigate the effects of impulse noise on the epithelial cells of the choroid plexus. MATERIAL and Forty Sprague Dawley rats were used and divided into the 2 groups as control and study groups. In the control group, the rats did not suffer noise ...
Givan Alice L - - 2011
A flow cytometer is an instrument that illuminates cells (or other particles) as they flow individually in front of a light source and then detects and correlates the signals from those cells that result from the illumination. In this chapter, each of the aspects of that definition will be described: ...
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