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Barnes P W - - 2010
We recently conducted a series of evaluations of the new UniCel DxH 800 Coulter Cellular Analysis System at Barnes-Jewish Hospital in St. Louis, Missouri. This report addresses issues relating to the flagging performance of the DxH 800, especially as it relates to the detection of blast cells. The DxH 800 ...
Kavanagh D M - - 2010
Intense research has been carried out in recent years into methods that aim to harvest fetal genetic material from maternal blood as substitutes to amniocentesis and chorionic villus sampling. Just over 30 years have past since the first fetal cells were separated from maternal blood using flow cytometry highlighting the ...
Sampimon Otlis - - 2010
The effect was quantified of coagulase-negative staphylococci (CNS) intramammary infections on quarter- and cow-level somatic cell count (SCC) and on bulk milk somatic cell count (BMSCC) in different BMSCC cohorts in Dutch dairy herds. Two datasets were used for this purpose. In the first dataset, on 49 randomly selected dairy ...
Batista Urska - - 2010
Cell detachment procedures can cause severe damage to cells. Many studies require cells to be detached before measurements; therefore, research on cells that have been grown attached to the bottom of the culture dish and later detached represents a special problem with respect to the experimental results when the properties ...
Dalbeth Nicola - - 2010
OBJECTIVE: To characterize the cellular architecture of the tophus and to determine the presence of cytokines implicated in the initiation and resolution of gouty inflammation. METHODS: Sixteen fixed, paraffin-embedded, uninfected tophus samples were surgically obtained from 12 patients with microscopically proven gout and were analyzed by quantitative immunohistochemistry. The number ...
Janssen William E - - 2010
BACKGROUND AIMS: Multiple cell-therapy products require density separation as a part of manufacturing. The traditional method for Ficoll separation, layering cell suspensions over Ficoll in tubes, followed by centrifugation and collection of cells from the interface, is too cumbersome and poses too high a risk of contamination for clinical-scale use. ...
Rerknimitr Rungsun - - 2010
BACKGROUND: Currently, decision to give antibiotics in spontaneous bacterial peritonitis (SBP) suspected patient depends mainly on the result of manual cell count, which requires significant waiting period. Recently, many reports on the efficacies of reagent strips and a few reports of automated cell count are available but there has been ...
de Jonge Robert - - 2010
BACKGROUND: We evaluated the body fluid (BF) mode on the new Sysmex XE-5000 analyzer. METHODS: Red (RBC) and white blood cell (WBC) (differential) counts of BFs (139 patient samples and 87 normal samples) were measured and compared to the Fuchs-Rosenthal chamber and stained cytospin slides. RESULTS: Extended cell counting using ...
Henderson Rita K - - 2010
The aim of this study was to compare the coagulation and flotation of different algae species with varying morphology and algogenic organic matter (AOM) composition in order to link physical and chemical algae characteristics to treatment. Microcystis aeruginosa (cyanobacteria), Chlorella vulgaris (green algae), Asterionella formosa and Melosira sp. (diatoms) were ...
Xu Baojun - - 2010
The aims of this work were to compare antiproliferation, antioxidant activities and total phytochemicals and individual isoflavone profiles in soy milk processed by various methods including traditional stove cooking, direct steam injection, direct ultrahigh temperature (UHT), indirect UHT, and a two-stage simulated industry method, and a selected commercial soy milk ...
Wang Nan - - 2010
A shotgun proteome analysis method and its performance for protein identification from 500 to 5000 cells are described. Sample preparation, which was done in one tube, involved the use of a surfactant (NP-40) for cell lysis, followed by acetone precipitation of the proteins. The resulting protein pellet was washed with ...
Wouters An - - 2010
The clonogenic assay is the method of choice to determine cell reproductive death after in vitro irradiation treatment. Traditionally, colony quantification has been performed by manual counting, a very laborious, time-consuming and rather subjective task. In this study, we compared manual counting by two skilled investigators with automated counting using ...
Paris A - - 2010
We evaluated the performance of the automated body fluid mode of the Sysmex XE-5000 series automated haematology analyzer and compared the performance of the automated method for obtaining white blood cell (WBC), red blood cell (RBC) counts and WBC differential counts with microscopic method. One hundred and seventy-four samples were ...
Harabajsa Suzana - - 2010
Bronchoalveolar lavage (BAL) fluid cells count provides information about presence or absence of interstitial lung diseases. BAL fluid samples were taken from 50 patients hospitalized in University Hospital for Lung Diseases "Jordanovac" in Zagreb, Croatia. The samples of BAL fluid were prepared by cytocentrifuge. From each sample two cytospin were ...
Huang Li-Chun - - 2010
A method using Cedex automatic cell counter (Innovatis) to determine the cell density and viability of a whole cell-based immunotherapy product has been developed and validated for the assay performance characteristics including specificity, accuracy, precision, linearity, range, and robustness. Instrument-to-instrument variation due to intrinsic differences in handmade flow cells was ...
Gao Ning - - 2010
In this paper, a cell separation technique has been explored using antibody-functionalized Ni nanowires. An antibody (anti-CD31) against mouse endothelial cells (MS1) was conjugated to the Ni nanowire surface through self-assembled monolayers (SAMs) and chemical covalent reactions. The measured cytotoxicity was negligible on the CD-31 antibody-functionalized nanowires by the tetrazolium ...
Lenders L M - - 2010
BACKGROUND/OBJECTIVE: Flexibility in sample processing may improve test utility of the quantitative antigen-specific T cell assay (T-SPOT.TB). We investigated whether delayed sample processing with and without the use of T-Cell Xtend, a proprietary reagent, impacted upon test accuracy. METHODS: Blood samples obtained from 363 sequentially recruited tuberculosis suspects or treated ...
De Smet Dieter - - 2010
The enumeration and identification of blood cells in body fluids offers important information for the diagnosis and treatment of various medical conditions. Manual microscopic methods (hemacytometer total cell count and cytocentrifuged differential count) have inherent analytic and economic disadvantages but are still considered the "gold standard" methods. We evaluated the ...
Omori Atsuko - - 2010
Placental/umbilical cord blood (CB) has been used increasingly not only for transplantations, but also in the field of life science research. However, little information is available on the biological characteristics of CB units collected in rural areas because no medical facilities are affiliated with CB banks. Little attention has been ...
Bruce Andrew T - - 2010
Suspensions of viable urothelial cells (UC) isolated from patient bladder biopsies often contain considerable amounts of extraneous materials comprised of cellular debris, dead and dying UC, and red blood cells. We have consistently observed an inversely proportional relationship between UC attachment efficiency and the amount of extraneous materials in the ...
Black David A - - 2010
Coordinate expression of CTGF and TGF-ss plays an important role in tissue fibrosis. Expression of these factors is increased under hypoxic conditions. Treatment of cells under hypoxic conditions with fructose-1,6-bisphosphate has been previously shown to decrease production of CTGF [1]. Mannose-6-phosphate (M6P) may function to down regulate fibrosis by bypassing ...
Guarner Jeannette - - 2010
BACKGROUND: Each institution sets specific parameters obtained by automated hematology analyzers to trigger manual counts. We designed a process to decrease the number of manual differential cell counts without impacting patient care. METHODS: We selected new criteria that prompt manual counts and studied the impact these changes had in 2 ...
Ren Xiaomin - - 2010
We introduce here a method for continuous intact cell detection and viability determination of individual trypan blue stained cells by CE with ultraviolet-visible dual-wavelength detection. To avoid cell aggregation or damage during electrophoresis, cells after staining were fixed with 4% formaldehyde and were continuously introduced into the capillary by EOF. ...
Pimentel J A - - 2010
In this work, we describe a segmentation cell method oriented to deal with experimental data obtained from 3D+t microscopical volumes. The proposed segmentation technique takes advantage of the pattern of appearances exhibited by the objects (cells) from different focal planes, as a result of the object translucent properties and its ...
Li Simon - - 2010
The increasing amounts of microscopy data generated in cell biology requires the development of automated tools for the quantitative analysis of images. Clumps of cells are difficult to segment due to the frequent lack of clear boundaries between cells and are often ignored, but communication between cells is an intrinsic ...
Schmidt Marcin T - - 2010
Microbial colonization is an important step in establishing pathogenic or probiotic relations to host cells and in biofilm formation on industrial or medical devices. The aim of this work was to verify the applicability of quantitative PCR (Real-Time PCR) to measure bacterial colonization of epithelial cells. Salmonella enterica and Caco-2 ...
Altug G - - 2010
AIMS: To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater. METHODS AND RESULTS: Hundred ...
Nikolaidis Ilias - - 2010
A case of a 69-year-old woman with myelofibrosis presenting with non-small cell lung cancer was reported at Sotiria General Hospital in Athens. She was administered bevacizumab infusions biweekly and a great decrease in the number of her white blood cells was observed. Such low levels of white blood cells had ...
Kachouie Nezamoddin N - - 2010
Methods of manual cell localization and outlining are so onerous that automated tracking methods would seem mandatory for handling huge image sequences, nevertheless manual tracking is, astonishingly, still widely practiced in areas such as cell biology which are outside the influence of most image processing research. The goal of our ...
Forero Manuel Guillermo MG Neurodevelopment Group, School of Biosciences, University of Birmingham, Birmingham, United - - 2010
Cell number changes during normal development, and in disease (e.g., neurodegeneration, cancer). Many genes affect cell number, thus functional genetic analysis frequently requires analysis of cell number alterations upon loss of function mutations or in gain of function experiments. Drosophila is a most powerful model organism to investigate the function ...
Hector A - - 2010
Induced sputum is the most commonly used method to analyze airway inflammation in cystic fibrosis (CF) patients ex vivo. Due to the complex matrix of the sample material, precise and reliable analysis of sputum constituents depends critically on preanalytical issues. Here we compared the commonly used method for sputum processing ...
Itzhaki-Alfia Ayelet - - 2009
BACKGROUND: The identification and isolation of human cardiac progenitor cells (hCPCs) offer new approaches for myocardial regeneration and repair. Still, the optimal source of human cardiac progenitor cells and the influence of patient characteristics on their number remain unclear. Using a novel method to isolate human cardiac progenitor cells, we ...
Riggio V - - 2009
The objectives of this study were to evaluate the effect of somatic cell count (SCC) on functional longevity and to estimate the heritability of functional longevity using survival analysis in Valle del Belice dairy sheep. A total of 4,880 lactations of 2,190 ewes from 11 flocks were used. In this ...
Moehlenbrock Michael J - - 2009
Enzymatic biofuel cells, which replace expensive metal catalysts with enzymes, are still in an early stage of development. This article details the analytical techniques that are often employed for evaluating and characterizing enzymatic biofuel cells and their corresponding bioanodes and biocathodes. (To listen to a podcast about this feature, please ...
Zaman Ferhana - - 2009
Ultra-scale down (USD) methodology developed by University College London for cell broth clarification with industrial centrifuges was applied to two common cell lines (NS0 and GS-CHO) expressing various therapeutic monoclonal antibodies. A number of centrifuges at various scales were used with shear devices operating either by high speed rotation or ...
Tahar Aissa Jamel - - 2009
This study showed that macrophages, B cells, and T cells occurred at different frequencies, localizations, and times after acute laser tympanic membrane perforation. Immunological cells were most prevalent in the proliferative mass adjacent to the annulus region. The cellular immunological response in freshly perforated eardrums was unexpectedly aggressive. To study ...
Reimann Sebastian - - 2010
Viability of probiotic bacteria is traditionally assessed by plate counting which has several limitations, including underestimation of cells in aggregates or chains morphology. We describe a quantitative PCR (qPCR)-based method for an accurate enumeration of viable cells of Bifidobacterium longum NCC2705 exhibiting different morphologies by measuring the mRNA levels of ...
Hirota Yusuke - - 2010
The separation characteristics of a wire-wire type dielectrophoretic (DEP) filter were evaluated using animal cells. The separation of cells with different activities was examined using a DEP filter. The specific growth rate of the cells in retention liquid was larger than that in permeation liquid. From the culture results of ...
Amato Domenico - - 2010
To compare two different techniques for preparation of pre-cut lamellar corneal tissue for Descemet stripping automated keratoplasty. Eight donor lenticules were created with a full pass of the microkeratome blade, which resulted in a posterior lamella and a free cap (anterior lamella off group (AL-off)). Contralateral donor lenticules were created ...
King D S - - 2010
To (i) develop a protocol that would eliminate or greatly reduce sporulation within Bacillus anthracis vegetative cells, and (ii) harvest an adequate number of cells and sufficient DNA suitable for molecular methods including Riboprint analysis and pulse field gel electrophoresis (PFGE). Seven strains of B. anthracis (Ames, French B2, Heluky, ...
Tait A S - - 2009
This article describes how a combination of an ultra scale-down (USD) shear device feeding a microwell centrifugation plate may be used to provide a prediction of how mammalian cell broth will clarify at scale. In particular a method is described that is inherently adaptable to a robotic platform and may ...
Alyassin Mohamad A MA Bio-Acoustic MEMS in Medicine Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, - - 2009
Lab-chip device analysis often requires high throughput quantification of fluorescent cell images, obtained under different conditions of fluorescent intensity, illumination, focal depth, and optical magnification. Many laboratories still use manual counting--a tedious, expensive process prone to inter-observer variability. The manual counting process can be automated for fast and precise data ...
Lievaart Jan - - 2009
Bulk milk somatic cell count (BMSCC) is a frequently used parameter to estimate the subclinical mastitis prevalence in a dairy herd, but it often differs considerably from the average SCC of all individual cows in milk. In this study, first the sampling variation was determined on 53 dairy farms with ...
Fong Chui-Yee - - 2009
Discontinuous density gradients have been shown to be very efficient in sperm enrichment in assisted reproductive technologies (ART). Percoll [polyvinylpyrrolidone (PVP)-coated silica] which is a colloidal agent commonly used in such gradients is now confined to only research purposes in ART because its PVP component was considered toxic for clinical ...
Fong Chui-Yee - - 2009
Discontinuous density gradients have been shown to be very efficient in sperm enrichment in assisted reproductive technologies (ART). Percoll [polyvinylpyrrolidone (PVP)-coated silica] which is a colloidal agent commonly used in such gradients is now confined to only research purposes in ART because its PVP component was considered toxic for clinical ...
Lee Jung-Lim - - 2009
The effect of refrigerated and frozen storage on the viability of Vibrio vulnificus was evaluated using cell suspensions (1x10(8) CFU/ml). Ethidium bromide monoazide (EMA) was utilized to selectively allow real-time (Rti) PCR amplification of target DNA from viable but not dead cells. Bacterial survivors from the EMA Rti-PCR were evaluated ...
van Straten M - - 2009
The objective of this study was to determine associations between body weight (BW) and body condition score (BCS) variables indicating a more severe negative energy balance in early lactation and events of somatic cell counts (SCC) >250,000 cells/mL and SCC >400,000 cells/mL in dairy cows. We studied lactations from 634 ...
Ordóñez-Gutiérrez L - - 2009
Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by ...
Martinez Viedma Pilar - - 2009
Enterocin AS-48 was tested against Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes in different kinds of desserts. The highest activity against S. aureus was detected in baker cream. However, in yogurt-type soy-based desserts and in gelatin pudding, AS-48 (175 arbitrary units [AU]/g) reduced viable cell counts of S. aureus by ...
Schmidt Michael - - 2009
BACKGROUND: According to German regulations and guidelines, residual red blood cells (rRBCs) and residual white blood cells (rWBCs) must number fewer than 3 x 10(9) cells/unit and 1 x 10(6) cells/unit in platelet concentrates (PCs), respectively. Due to low levels of residual cells in final products, there is still a ...
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