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Savariz Franciele Cristina FC Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP: 87020-900 Maringá, PR, - - 2014
A series of novel 1-(substituted phenyl)-3-(2-oxo-1,3,4-oxadiazol-5-yl) β-carbolines (4a-e) and the corresponding Mannich bases 5-9(a-c) were synthesized and evaluated for their in vitro antitumor activity against seven human cancer cell lines. Compounds of 4a-e series showed a broad spectrum of antitumor activity, with GI50 values lower than 15μM for five cell ...
Ramirez Yulian P YP Biochemistry and Molecular Pharmacology, University of Massachusetts Medical - - 2014
The cellular responses to two new temozolomide (TMZ) analogues, DP68 and DP86, acting against glioblastoma multiforme (GBM) cell lines and primary culture models are reported. Dose-response analysis of cultured GBM cells revealed that DP68 is more potent than DP86 and TMZ and that DP68 was effective even in cell lines ...
Yanagihara Sae S Microbiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan. Electronic address: - - 2014
To determine the reason for the low levels of Surface layer protein A (SlpA) on CP23 cells, which might play a crucial role in the immunomodulatory effect of Lactobacillus acidophilus, the DNA sequence of the slpA gene of CP23 and L-92 strains, including the upstream region, were analyzed. Unexpectedly, there ...
Jang Dae-Ho DH School of Mechanical Engineering, Korea University, Seoul 136-701, Republic of Korea. Electronic address: - - 2014
A sensitive method for selectively detecting insulin-like growth factor-binding protein 7 (IGFBP7) over a wide range of concentrations based on the surface plasmon resonance (SPR) biosensing techniques is described. IGFBP7 has been shown to regulate cell proliferation, cell adhesion, cellular senescence, apoptosis, and angiogenesis in several different cancer cell lines. ...
Pan Ming - - 2014
This paper describes the design and synthesis of amphiphilic silica nanoparticles for the stabilization of aqueous drops in fluorinated oils for applications in droplet microfluidics. The success of droplet microfluidics has thus far relied on one type of surfactants for the stabilization of drops. Surfactants are known to have two ...
Atkuri Kondala R KR Pfizer - - 2014
Advanced single cell analysis technologies (e.g. Mass Cytometry) that help in multiplexing cellular measurements in limited volumes of primary cells are critical in bridging the discovery efforts to successful drug approval. Mass cytometry is the state-of-the-art technology in multi-parametric single-cell analysis. Mass cytometers (also known as CyTOF or Cytometry by ...
Zechel Sabrina - - 2014
BackgroundCortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventricular zone of the MGE, but whether the underlying subventricular and mantle regions of the MGE are spatially patterned has ...
Kondo Yuki Y Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan - - 2014
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular developmen in detail. Here, we establish a novel in vitro experimental system, in which vascular development is induced in ...
Wang Yurong Y Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China, - - 2014
Concerns about the risk of titanium dioxide nanoparticles (TiO2 NPs) to human health and environment are gradually increasing due to their wide range of applications. In this study, cytotoxicity, DNA damage, and apoptosis induced by TiO2 NPs (5 nm) in A549 cells were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed the ...
Vanhoutteghem Amandine A Laboratoire de physiologie cérébrale, Centre National de la Recherche Scientifique, Université Paris Descartes, UMR 8118, Paris, - - 2014
Absence of mitosis and meiosis are distinguishing properties of male germ cells during late fetal and early neonatal periods. Repressors of male germ cell meiosis have been identified, but mitotic repressors are largely unknown, and no protein repressing both meiosis and mitosis is known. We demonstrate here that the zinc-finger ...
Norouzi Mohammad M Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, - - 2014
The novel strategies of skin regenerative treatment are aimed at the development of biologically responsive scaffolds capable of delivering multiple bioactive agents and cells to the target tissues. In this study, nanofibers of poly (lactic-co-glycolic acid) (PLGA) and gelatin were electrospun and the effect of parameters viz polymer concentration, acid ...
Takamiya Rina R Department of Biochemistry, Sapporo Medical University School of Medicine, Japan. Electronic address: - - 2014
It has been well documented that activation of the ErbB3-PI3K-Akt pathway is implicated in tumor survival and progression. We previously demonstrated that the single N-glycan deletion mutant of soluble ErbB3 protein (sErbB3 N418Q) attenuates heregulin β1-induced ErbB3 signaling. The active PI3K-Akt pathway augments the nuclear accumulation of hypoxia inducible factor ...
Ge Xiaojun X Department of Medical Oncology and Laboratory of Signal Transduction and Molecular Targeted Therapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to establish, characterize and elucidate the potential mechanisms of acquired gefitinb resistance, using the A549 human lung cancer cell line. A gefitinib‑resistant A549 sub‑clone was established by exposure to escalating gefitinib concentrations over a period of 16‑24 months. Half maximal inhibitory concentration (IC50) values were ...
Sun Chen - - 2014
Quantum dots (QDs) have found a wide range of biological applications as fluorophores due to their extraordinary brightness and high photostability that are far superior to conventional organic dyes. These traits are particularly appealing for studying cell biology under cellular autofluorescence background and with long observation period. However, it remains ...
Grecco Simone Dos S SD Centro de Ciências Naturais e Humanas, Universidade Federal do ABC/SP , Brazil - - 2014
Abstract Context: Nectandra (Lauraceae) species have been used in folk medicine as an antidiarrheal, analgesic, antifungal, etc., and have many pharmacological proprieties. Objective: Investigation of the chemical composition and cytotoxicity of essential oil from Nectandra leucantha Nees & Mart. leaves. This is the first study involving N. leucantha reported in ...
Tabbaa Suzanne S Department of Bioengineering and Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, - - 2014
The major limitation of large tissue-engineered constructs used for bone regeneration is the lack of vasculature and, therefore, lack of transport of essential nutrients, chemical factors and progenitor cells. Research approaches to improve the transport properties of large scaffolds focus on using angiogenic factors and vasculogenic cells to create new ...
Khattar Vinayak V Southern Research Institute, Birmingham, AL, - - 2014
Constitutive levels of Cks1 protein are very high in mammary carcinoma tissue and in breast tumor cell lines. However, despite being transcribed at relatively high levels, Cks1 protein is very low in normal mammary tissue. Also, basal Cks1 is barely detectable in primary human mammary epithelial cells (HMECs). Epoximicin, a ...
Bongard Dirk D Institute of Chemistry of New Materials, University of Osnabrück, Barbarastr. 7, D-49076 Osnabrück, Germany. - - 2014
The synthesis of two types of viologen dendrimers with peripheral carboxyl groups is described. Their interaction with plasmid DNA and CT-DNA and the influence of time evolution and electrolyte on dendriplex formation have been electrochemically investigated. A negative potential shift appearing in the cyclic voltammograms of the dendrimers indicates dendriplex ...
Xu Yanping Y Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA, - - 2014
A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the ...
Shankaraiah Nagula N Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad 500 037, India. Electronic address: - - 2014
A series of new tetrahydro-β-carboline-hydantoin hybrids have been designed and synthesized based on the structure of the known Eg5 inhibitor HR22C16. These compounds have been evaluated for their anticancer activity against lung (A549), cervical (ME180, HeLa), prostate (PC-3) and breast (MCF-7) cancer cell lines by MTT assay. These hybrids have ...
Ramírez G A GA Unit of Histology and Veterinary Pathology, Institute for Animal Health, Veterinary College, University of Las Palmas de Gran Canaria, Campus Universitario Cardones, 45413 Arucas, Las Palmas, Spain. Electronic address: - - 2014
Merkel cells (MCs) are specialized skin receptors characterized by their particular location and close association with nerve terminals. They also are cells with a presumptive neuroendocrine function and are considered as part of the diffuse neuroendocrine system. By using commercially available monoclonal and polyclonal antibodies in samples of dog skin, ...
Pegeot Mathieu M Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France CNRS, IBS, F-38027 Grenoble, France CEA, DSV, IBS, F-38027 Grenoble, - - 2014
Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding depends on the level of HS sulfation and epimerization which are cell specific and dynamically regulated. Characterization of this molecule, however, has been restricted to oligosaccharide fragments available in large amount for ...
Schwanbeck Ralf R Institute of Biochemistry, Medical Faculty, University of Kiel, Olshausenstr. 40, 24098, Kiel, - - 2014
The Notch pathway is a highly conserved cell-cell communication pathway in metazoan involved in numerous processes during embryogenesis, development, and adult organisms. Ligand-receptor interaction of Notch components on adjacent cells facilitates controlled sequential proteolytic cleavage resulting in the nuclear translocation of the intracellular domain of Notch (NICD). There it binds ...
Edwald Elin E Program in Chemical Biology, University of Michigan, Ann Arbor, - - 2014
Many cell types undergo a hypoxic response in the presence of low oxygen, which can lead to transcriptional, metabolic, and structural changes within the cell. Many biophysical studies to probe the localization and dynamics of single fluorescently labeled molecules in live cells either require or benefit from low-oxygen conditions. In ...
Panikkanvalappil Sajanlal R - - 2014
Owing to the dynamic and complex nature of mitosis, precise and timely executions of biomolecular events are critical for high fidelity cell division. In this context, visualization of such complex events at the molecular level can provide vital information on the biomolecular processes in abnormal cells. Here, we explored the ...
Chandler J H JH School of Mechanical Engineering, University of Leeds, Leeds LS2 9JT, United Kingdom. Electronic address: - - 2014
Biogalvanic characterisation is a promising method for obtaining health-specific tissue information. However, there is a dearth of understanding in the literature regarding the underlying galvanic cell, electrode reactions and their controlling factors which limits the application of the technique. This work presents a parametric electrochemical investigation into a zinc-copper galvanic ...
Zhang Haifeng H Department of Pathology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: - - 2014
Cell volume regulation is a fundamental activity to maintain cell survival, and aquaporins and chloride channels play important roles in this process. However, the interactions between these channels are far from clear. In this study, the interactions between AQP-3 and ClC-3 were investigated in CNE-1 and CNE-2Z nasopharyngeal carcinoma cells, ...
Lauer Florian M FM Membrane Dynamics, Department of Biology, Technische Universität Darmstadt, Schnittspahnstrasse 3-5, 64287 Darmstadt, - - 2014
From the onset of the first microscopic visualization of single fluorescent molecules in living cells at the beginning of this century, to the present, almost routine application of single molecule microscopy, the method has well-proven its ability to contribute unmatched detailed insight into the heterogeneous and dynamic molecular world life ...
Saini Ravi Kanth Rao RK Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden; Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, - - 2014
Neuroblastoma develops through processes which include cellular dedifferentiation. Ability of tumors to form spheroids is one of the manifestations of dedifferentiation and carcinogenic transformation. To study mechanisms of dedifferentiation of neuroblastoma cells, we generated spheroids and performed a proteomics study to compare the spheroids with parental SK-N-BE2 cells. We observed ...
Jung Yong-Gyun YG Quantamatrix Inc, 104-213, Seoul National University, Seoul 151-742, Republic of - - 2014
A variety of systems have been developed to study biofilm formation. However, most of the systems are based on the surface-attached growth of microbes under shear stress. In this study, we designed a microfluidic channel device, called an MAC (microfluidic agarose channel), and found that microbial cells in the MAC ...
Shannahan Jonathan H JH * Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045, - - 2014
Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming ...
Chen Jin J Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. - - 2014
The aim of the present study was to evaluate the association between DNA methylation and multidrug resistance (MDR) in glioma and identify novel effectors responsible for MDR in human gliomas. An MDR glioma cell line, SGH‑44/ADM, was developed using adriamycin (ADM) impulse treatment. Cryopreservation, recovery and withdrawal were performed to ...
Wang Huan H Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Heping, Shenyang, Liaoning 110004, - - 2014
Available evidence on the proliferation-promoting effect of HE4 remains controversial, and few studies have been carried out on the molecular mechanism of chemoresistance mediated by HE4. The aim of the present study was to investigate the influence of exogenous recombinant HE4 protein on proliferation and resistance to carboplatin in ovarian ...
Adams David William DW Centre for Bacterial Cell Biology, Baddiley-Clark Building, Medical School, Newcastle University, Richardson Road, Newcastle Upon Tyne, NE2 4AX, United - - 2014
Division site selection presents a fundamental challenge to all organisms. Bacterial cells are small and the chromosome (nucleoid) often fills most of the cell volume. Thus, in order to maximise fitness and avoid damaging the genetic material, cell division must be tightly co-ordinated with chromosome replication and segregation. To achieve ...
Zabka Aneta A Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland. Electronic address: - - 2014
Long-term treatment of Allium cepa seedlings with low concentration of hydroxyurea (HU) results in a disruption of cell cycle checkpoints, leading root apex meristem (RAM) cells to an abnormal organization of nuclear structures forming interphase (I) and mitotic (M) domains of chromatin at opposite poles of the nucleus. Thus far, ...
Madeo Marianna M From the Sanford Children's Health Research Center, Sanford Research, Sioux Falls, South Dakota 57104 - - 2014
SV2A is a synaptic vesicle membrane protein expressed in neurons and endocrine cells and involved in the regulation of neurotransmitter release. Although the exact function of SV2A still remains elusive, it was identified as the specific binding site for levetiracetam, a second generation antiepileptic drug. Our sequence analysis demonstrates that ...
Li Yin Y Laboratory of Reproductive and Developmental Toxicology - - 2014
Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines in circulation, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. ...
Sang Xiao-Jing XJ Key Laboratory of Polyoxometalate Science of Ministry of Education, Department of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China. wangeb889@nenu.edu.cn - - 2014
Two novel open-chain carboxyethyltin decorated sandwich-type germanotungstates have been successfully synthesized. They could markedly increase the electrocatalytic activity of single-walled carbon nanotubes toward triiodide reduction when assembled into composite electrodes, which have shown a conversion efficiency of 6.32% that is comparable to that of Pt electrodes (6.29%) when used as ...
Berger Gregory G Department of Infectious Diseases, Faculty of Life Sciences and Medicine, King's College London, Guy's Hospital, London SE1 - - 2014
The accessory gene vpr, common to all primate lentiviruses, induces a potent G2/M arrest in cycling cells. A recent study showed that HIV-1 Vpr mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi Anemia DNA repair pathway. To confirm these observations, we have examined ...
Ortogero Nicole N University of Nevada, Reno, United - - 2014
PIWI-interacting RNAs (piRNAs) are small noncoding RNAs that bind PIWI family proteins exclusively expressed in the germ cells of mammalian gonads. MIWI2-associated piRNAs are essential for silencing transposons during primordial germ cell development, and MIWI-bound piRNAs are required for normal spermatogenesis during adulthood in mice. Although piRNAs have long been ...
Hwang Kevin K Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 - - 2014
DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, ...
Xu Xun X Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195, Berlin, - - 2014
Directing stem cells towards a desired location and function by utilizing the structural cues of biomaterials is a promising approach for inducing effective tissue regeneration. Here, the cellular response of human adipose-derived mesenchymal stem cells (hADSCs) to structural signals from microstructured substrates comprising arrays of square-shaped or round-shaped microwells is ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, Council of Science and Industrial Research, Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007 (India); Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad-500 037 (India). - - 2014
A series of chalcone conjugates featuring the imidazo[2,1-b]thiazole scaffold was designed, synthesized, and evaluated for their cytotoxic activity against five human cancer cell lines (MCF-7, A549, HeLa, DU-145 and HT-29). These new hybrid molecules have shown promising cytotoxic activity with IC50 values ranging from 0.64 to 30.9 μM. Among them, (E)-3-(6-(4-fluorophenyl)-2,3-bis(4-methoxyphenyl)imidazo[2,1-b]thiazol-5-yl)-1-(pyridin-2-yl)prop-2-en-1-one ...
Zhang Shaolian S Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. typh@jnu.edu.cn - - 2014
A highly sensitive and recyclable quartz crystal microbalance (QCM) biosensor was developed using chitosan (CS) and folic acid (FA), generating conjugates that are selectively recognized by MCF-7 cancer cell over-expressed folic acid receptors. The prepared CS-FA conjugate was characterized by UV-vis spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy ...
Reyes de Mochel Nabora Soledad NS Department of Developmental & Cell Biology, University of California, Irvine, CA 92697-2300 - - 2014
The mechanisms regulating cell division during development of the mouse pre-implantation embryo are poorly understood. We have investigated whether bone morphogenetic protein (BMP) signaling is involved in controlling cell cycle during mouse pre-implantation development. We mapped and quantitated the dynamic activities of BMP signaling through high-resolution immunofluorescence imaging combined with ...
Wahab Rizwan R A.R.Al-Jeraisy, Chair for DNA Research, Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: - - 2014
Nanoscale gold particles (Au-NPs) with a diameter below 20nm are notably important candidates for various important applications because of their extraordinary quantum size effects. Their high surface area-to-volume ratio facilitates their very high reactivities; therefore, they can be utilised in different ways in biomedical applications. For example, these nanoparticles can ...
Gallagher Kimberly L KL Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104; email: gallagkl@sas.upenn.edu , - - 2014
Development in multicellular organisms requires the coordinated production of a large number of specialized cell types through sophisticated signaling mechanisms. Non-cell-autonomous signals are one of the key mechanisms by which organisms coordinate development. In plants, intercellular movement of transcription factors and other mobile signals, such as hormones and peptides, is ...
Becker Yvonne Y Massey University, Institute of Fundamental Sciences, Palmerston North, New Zealand ; - - 2014
Epichloё festucae is a mutualistic symbiont that systemically colonises the intercellular spaces of Lolium perenne leaves to form a highly structured and interconnected hyphal network. In an Agrobacterium tumefaciens T-DNA forward genetic screen we identified a mutant TM1066 that had a severe host interaction phenotype, causing stunting and premature senescence ...
Havrylov Serhiy S Goodman Cancer Research Centre, McGill University, H3A1A3, Montreal, QC, Canada; Department of Medicine, McGill University, H3A1A3, Montreal, QC, - - 2014
Acquired capacity of cancer cells to penetrate through the extracellular matrix (ECM) of surrounding tissues is a prerequisite for tumour metastatic spread - the main source of cancer-associated mortality. Through combined efforts of many research groups we are beginning to understand that the ability of cells to invade through the ...
Waki Michihiko M Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, - - 2014
Lipids comprise the primary component of cell membranes. Imaging mass spectrometry is increasingly being used to visualize membranous lipids in clinical specimens, and it has revealed that abnormal lipid metabolism is related to the development of diseases. To characterize cell populations which are rare and sparsely localized in tissues, we ...
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