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McAlpine Jennifer A - - 2014
Many advanced human tumors, including hepatocellular carcinomas (HCC) are auxotrophic for arginine due to down-regulation of argininosuccinate synthetase (ASS1), the rate-limiting enzyme in arginine synthesis. The arginine-lowering agent PEGylated arginine deiminase (ADI-PEG 20) has shown efficacy as a monotherapy in clinical trials for treating arginine-auxotrophic tumors and is currently being ...
Lee Hangnoh - - 2014
Structural rearrangements of the genome resulting in genic imbalance due to copy number change are often deleterious at the organismal level, but are common in immortalized cell lines and tumors, where they may be an advantage to cells. In order to explore the biological consequences of copy number changes in ...
Mirigian Lynn S LS Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA - - 2014
Abstract Disruptions in procollagen synthesis, trafficking and secretion by cells occur in multiple connective tissue diseases. Traditionally, these disruptions are studied by pulse-chase labeling with radioisotopes. However, significant DNA damage, excessive accumulation of reactive oxygen species and formation of other free radicals have been well documented in the literature at ...
Mayrhofer Patrick P Department of Biotechnology/Vienna Institute of BioTechnology (BOKU-VIBT), University of Natural Resources and Life Sciences (Vienna), Muthgasse 18, A-1190, Vienna, - - 2014
Over the years, Chinese hamster ovary (CHO) cells have emerged as the major host for expressing biotherapeutic proteins. Traditional methods to generate high-producer cell lines rely on random integration(s) of the gene of interest but have thereby left the identification of bottlenecks as a challenging task. For comparison of different ...
Vazquez Hector M HM *Department of Biology, University of Fribourg, - - 2014
Temperature sensitive cdc1(ts) mutants are reported to stop the cell cycle upon shift to 30°C in early G2, i.e. as small budded cells having completed DNA replication but unable to duplicate the spindle pole body. A recent report showed that PGAP5, a human homologue of CDC1, acts as a phosphodiesterase ...
Kourakis Matthew J MJ Department of Molecular, Cell and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, 93106, - - 2014
Despite its importance in development and physiology the planar cell polarity (PCP) pathway remains one of the most enigmatic signaling mechanisms. The notochord of the ascidian Ciona provides a unique model for investigating the PCP pathway. Interestingly, the notochord appears to be the only embryonic structure in Ciona activating the ...
Park Eun-Jung EJ Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Korea, - - 2014
Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ...
Mikeš J J Institute of Biology and Ecology, Faculty of Sciences, P. J. Šafárik University in Košice, Košice, Slovakia. - - 2014
Labelling of DNA in replicating cells using 5-bromo-2´-deoxyuridine (BrdU) is widely used, however the rapid clearance and metabolisation of BrdU in the living organism is a critical issue. Although the pharmacokinetic of BrdU in experimental animals is empirically approximated, the exact time-curve remains unknown. Here we present novel method for ...
Jung In-Soo IS College of Life Sciences and Biotechnology, Korea University, Seoul, - - 2014
Extremely low-frequency magnetic fields (ELF-MFs) affect various cellular processes and systems, such as cell proliferation, differentiation and metabolic pathways. The present study investigated ELF-MFs effect on nerve growth factor (NGF) induced neuronal differentiation of PC12 cells using proteomic applications to understand its role in the enhancement of neuronal differentiation. After ...
Liang Quanfeng Q State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, - - 2014
Engineering microorganisms capable of accumulating multiple products are sometimes attractive because they yield several advantages in balancing the in vivo metabolic flux and restoring the optimal cell physiology. With the development of metabolic engineering and synthetic biology, numerous strategies for minimizing the substrate waste, optimizing the product portfolios, and maximizing ...
Teh J T JT Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, - - 2014
Isoprenylcysteine carboxylmethyltransferase (Icmt) catalyzes the last of the three-step posttranslational protein prenylation process for the so-called CaaX proteins, which includes many signaling proteins, such as most small GTPases. Despite extensive studies on Icmt and its regulation of cell functions, the mechanisms of much of the impact of Icmt on cellular ...
Piotrowska U U Department of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Warsaw, - - 2014
The endocrine activity of the thyroid gland is accomplished by its follicular and parafollicular cells. In these cells, numerous G proteins-dependent pathways are active and potentially could be regulated by a 33-kDa cytoplasmic protein phosducin, which interacts with the Gβ subunit and may compete with Gα or Gβγ dimer effectors. ...
Podlipec Rok - - 2014
Understanding biocompatibility of materials and scaffolds is one of the main challenges in the field of tissue engineering and regeneration. Complex nature of cell-biomaterial interaction requires extensive preclinical functionality testing by studying specific cell responses to different biomaterial properties, from morphology and mechanics to surface characteristics at the molecular level. ...
Kuang Yi Y Brandeis University, United - - 2014
Emerging evidence reveals that prion-like structures play important roles to maintain the well-being of cells. While self-assembly of small molecules also afford prion-like nanofibrils (PriSM), little is known on the functions and mechanisms of PriSM. Previous works demonstrated that PriSM formed by a dipeptide derivative selectively inhibit the growth of ...
Balish Mitchell F MF Department of Microbiology, Miami University, Oxford, OH 45056, USA - - 2014
In recent decades, bacterial cell biology has seen great advances, and numerous model systems have been developed to study a wide variety of cellular processes, including cell division, motility, assembly of macromolecular structures, and biogenesis of cell polarity. Considerable attention has been given to these model organisms, which include Escherichia ...
Marie Dominique D Sorbonne Universités UPMC (PARIS 06), UMR 7144 and FR2424, Station Biologique de Roscoff, Place Georges Teissier, 29688, Roscoff, France; CNRS, UMR 7144 and FR2424, Station Biologique de Roscoff, Place Georges Teissier, 29688, Roscoff, - - 2014
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of ...
Ding Xiaoyun X Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA - - 2014
Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and efficiency of currently available ...
Luo Baiwen B School of Materials Science and Engineering, Nanyang Technological University, Singapore, - - 2014
Natural materials are promising alternatives to synthetic materials used in tissue engineering applications as they have superior biocompatibility and promote better cell attachment and proliferation. Ovalbumin, a natural polymer found in avian egg white, is an example of a nature-derived material. Despite the availability and reported biocompatibility of ovalbumin, limited ...
Chung Jinyang J Center for Environment, Health and Welfare Research, Department of Energy and Environmental Engineering, Korea University of Science and Technology (UST), Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of - - 2014
Fast and accurate pathogen detection in aquatic environments is challenging in many biomedical studies and microbial diagnostic applications. In this study, we developed a real-time, continuous, and non-destructive single cell detection method using target specific aptamer-conjugated fluorescent nanoparticles (A-FNPs) and an optofluidic particle-sensor platform. A-FNPs selectively bound to the surfaces ...
Dunlop E A EA Institute of Cancer and Genetics, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: - - 2014
Mechanistic target of rapamycin (mTOR) functions as a key homeostatic regulator of cell growth and orchestrates whether anabolic or catabolic reactions are favoured. mTOR complex 1 (mTORC1) manages multiple biosynthetic pathways and promotes cell growth when nutrients are in plentiful supply. Many advances have been made over the last decade ...
Maza Itay I The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, - - 2014
Xenopus oocytes can epigenetically reprogram mouse somatic cells toward totipotency. In this issue, Jullien et al. (2014) now describe rapid, interdependent molecular events that facilitate this reprogramming.
Zou Shu-Ping SP Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of - - 2014
The enantioselective resolution of (R,S)-2,3-dichloro-1-propanol ((R,S)-DCP) to (S)-DCP by whole cells of a recombinant Escherichia coli expressing halohydrin dehalogenase (HHDH) activity was limited by product inhibition. To solve this problem to improve the productivity of (S)-DCP, an n-heptane-aqueous biphasic system was adopted in this work. The influential operational parameters including ...
Shinagawa Masaaki M Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan; Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, - - 2014
The precise identities of the virulence factors of Pseudomonas aeruginosa after bacteriolysis are still unknown. In the present study, we identified PA0423 protein, which was isolated from the Pseudomonas PAO-1 strain, as the factor responsible for cytotoxicity in lung epithelial cells. Whole bacterial cell lysate of P. aeruginosa PAO-1 caused ...
Wu Pengxiang - - 2014
Water permeability of the plasma membrane plays an important role in making optimal cryopreservation protocols for different types of cells. To quantify water permeability effectively, automated cell volume segmentation during freezing is necessary. Unfortunately, there exists so far no efficient and accurate segmentation method to handle this kind of image ...
Stahl John S JS Louis Stokes Cleveland Dept. of Veterans Affairs Medical Center - - 2014
Mutation of the Cacna1a gene for the P/Q (CaV2.1) calcium channel invariably leads to cerebellar dysfunction. The dysfunction has been attributed to disrupted rhythmicity of cerebellar Purkinje cells, but the hypothesis remains unproven. If irregular firing rates cause cerebellar dysfunction, then the irregularity and behavioral deficits should co-vary in a ...
Nowotarski Stephanie H SH *Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC - - 2014
Actin-based protrusions are important for signaling and migration during development and homeostasis. Defining how different tissues in vivo craft diverse protrusive behaviors using the same genomic toolkit of actin regulators is a current challenge. The actin elongation factors Diaphanous and Enabled both promote barbed-end actin polymerization, and can stimulate filopodia ...
Macaulay Angus D - - 2014
Even after several decades of quiescent storage in the ovary, the female germ cell is capable of reinitiating transcription to build its reserves that are essential to support early embryonic development. In the current model of mammalian oogenesis, there exists bilateral communication between the gamete and the surrounding cells that ...
Cambré Alexander A Laboratory of Gene Technology, Department Biosystems, KU Leuven, B-3001 Heverlee, Belgium; Laboratory of Food Microbiology, Department of Microbial and Molecular Systems, KU Leuven, B-3001 Heverlee, - - 2014
Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the ...
Elliott Thomas S TS Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United - - 2014
The site specific, co-translational introduction of unnatural amino acids into proteins produced in cells has been facilitated by the development of the pyrrolysyl-tRNA synthetase/tRNACUA pair. This pair can now be used to direct the site-specific incorporation of designer amino acids in E. coli, yeast, mammalian cells, and animals (the worm, ...
Oikawa Shino S Department of Physiology, Nippon Medical School Graduate School of Medicine, Tokyo, - - 2014
Background/Aims: We previously suggested that a non-neuronal cholinergic system modulates energy metabolism through the mitochondria. However, the mechanisms responsible for making this system crucial remained undetermined. Methods: In this study, we developed a fusion protein expression vector containing a luciferase gene fused to the folic acid receptor-α gene. Results: This ...
Fu Yun Y Department of Molecular Biology and Biochemistry, Xinxiang Medical University, Xinxiang, Henan, P.R. - - 2014
The Mannich base containing ciprofloxacin and kojic acid structural units was prepared and evaluated in antitumor activity. The enhancement in antitumor activity was observed both from the Mannich base (IC50: 103.3±5.0 µM for HepG2, 87.9±8.0 µM for HCT-116 cell) and its copper complex (IC50: 11.5±1.8 µM for HepG2, 44.4±2.5 µM for HCT-116 cell) compared ...
Xu Jian J Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR - - 2014
A novel probe incorporating quaternized 4-pyridinium group into a BODIPY molecule was synthesized and studied for the selective detection of fluoride ions (F(-)) in aqueous solution. The design was based on a fluoride-specific desilylation reaction and the "Turn-On" fluorescent response of probe 1 to F(-) was ascribed to the inhibition ...
Ungvarsky Jan J Department of Organic Chemistry, Moyzesova 11, 04001 Kosice, Slovak - - 2014
A novel series of trisubstituted acridines were synthesized with the aim of mimicking the effects of BRACO19. These compounds were synthesized by modifying the molecular structure of BRACO19 at positions 3 and 6 with heteroacyclic moieties. All of the derivatives presented in the study exhibited stabilizing effects on the human ...
Pinzón Plinio Jesús - - 2014
We present an experimental approach for the fast measurement of twisted nematic (TN) liquid crystal (LC) cells' parameters. It is based on the spectral measurements of the light transmitted by the system polarizer-reference wave plate-LC cell-analyzer. The cell parameters are obtained by fitting the theoretical model to the experimental data. ...
Nakkala Jayachandra Reddy JR Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605 014, - - 2014
Nanomedicine utilize biocompatible nanomaterials for diagnostic and therapeutic purposes. This study reports the synthesis of silver nanoparticles using aqueous rhizome extract of Acorus calamus (ACRE) and evaluation of antioxidant, antibacterial as well as anticancer effects of synthesized A. calamus silver nanoparticles (ACAgNPs). The formation of ACAgNPs was confirmed by UV-visible ...
García Esther E Department of Cellular and Molecular Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus UAM Cantoblanco, Darwin 3, 28049 Madrid, Spain. Electronic address: - - 2014
Actin filament assembly and reorganisation during cell migration and invasion into extracellular matrices is a well-documented phenomenon. Among actin-binding proteins regulating its polymerisation, the members of the WASP (Wiskott Aldrich Syndrome Protein) family are generally thought to play the most significant role in supporting cell invasiveness. In situ, cytosolic N-WASP ...
Re Angela A Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Via delle Regole 101, I38123 Trento, Italy; Center for Biological Sequence Analysis, Technical University of Denmark, Kemitorvet, DK2800 Lyngby, - - 2014
The shortage of molecular information on cell cycle changes along embryonic stem cell (ESC) differentiation prompts an in silico approach, which may provide a novel way to identify candidate genes or mechanisms acting in coordinating the two programs. We analyzed germ layer specific gene expression changes during the cell cycle ...
Kota Swathi S Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400 084, India. - - 2014
PprA, a pleiotropic protein involved in radioresistance of Deinococcus radiodurans was detected in multiprotein DNA processing complex identified from this bacterium. pprA mutant expressing GFP-PprA could restore its wild type resistance of γ radiation. Under normal conditions, GFP-PprA expressing cells showed PprA localization on both septum trapped nucleoids (STN) and ...
He Min - - 2014
To describe the tissue distribution, density, and the morphological characteristics of 4 kinds of endocrine cells in the digestive tract of the Chinese yellow quail (Coturnix japonica). The streptavidin-biotin-peroxidase complex immunohistochemical method was used to identify the distribution of somatostatin (SS), serotonin (5-HT), gastrin and neuropeptide Y (NPY) in digestive ...
Meisenberg Cornelia C The Wellcome Trust DNA Repair Group, Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, - - 2014
Small cell lung cancer (SCLC) is one of the most challenging tumors to treat due to high proliferation rate, early metastatic dissemination and rapid development of chemotherapy resistance. The current treatment protocols involve the use of topoisomerase 1 (TOP1) poisons such as irinotecan and topotecan in combination with platinum-based compounds. ...
Kashiba Misato M School of Health Sciences, Tokyo University of Technology, 1404-1 Katakura-cho, Hachioji, Tokyo 192-0982, - - 2014
Coenzyme Q10 (CoQ10) is a key component of the mitochondrial electron transfer chain and is one of the most important cellular antioxidants. We previously reported that glycoprotein saposin B (SapB) binds CoQ10 in human cells. To elucidate the physiological role of SapB and its precursor, prosaposin (Psap), we prepared stable ...
Qin Jun - - 2014
The cells continuously secrete a large number of different types of microvesicles including macro-and micro-molecules into the extracellular fluids. One of them are exosomes, which are nano-sized vesicles capable of transferring the DNAs, microRNAs, non-coding RNAs and lipids with or without direct cell to cell contact, so representing the novel ...
Chandrasekaran Dhivya D Post Graduate and Research Department of Chemistry, Seethalakshmi Ramaswami College, Tiruchirappalli-620 002, - - 2014
Novel bicyclo[3.3.1]nonane derivatives were synthesized by an efficient methodology from acetoacetanilide, 2-methoxy and 4-methoxyacetoacetanilides, 1,3,5-trinitrobenzene and triethylamine. The structures of the compounds were characterized by UV/Visible, FTIR, (1)H NMR and 2D-correlation spectroscopy analysis. The in vitro cytotoxic studies were performed using Ehrlich Ascites Carcinoma cell line by Trypan blue dye ...
Lumetti S S Department of Biomedical, Biotechnological, and Translational Sciences, University of Parma, - - 2014
Rough titanium surfaces enhance the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of GSK3b-inhibitor (2'Z,3'E)- 6-Bromoindirubin-3'-oxime (BIO) on osteoblastic differentiation on titanium surfaces with different topography and wettability. C2C12 cells were plated on pickled, acid-etched/sand-blasted (SLA), modified hydrophilic SLA titanium ...
Alvarez-González Begoña B Mechanical and Aerospace Engineering Department, University of California, San Diego, La Jolla, CA, - - 2014
Migrating cells exert traction forces when moving. Amoeboid cell migration is a common type of cell migration that appears in many physiological and pathological processes and is performed by a wide variety of cell types. Understanding the coupling of the biochemistry and mechanics underlying the process of migration has the ...
Khanna Rajnish R Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA 94305, - - 2014
Reorganization of the cortical microtubule cytoskeleton is critical for guard cell function. Here, we investigate how environmental and hormonal signals cause these rearrangements and find that COP1, a RING-finger-type ubiquitin E3 ligase, is required for degradation of tubulin, likely by the 26S proteasome. This degradation is required for stomatal closing. ...
Vickers Dwayne A L DA The Rowland Institute, Harvard - - 2014
Electroporation has received increasing attention in the past years, because it is a very powerful technique for physically introducing non-permeant exogenous molecular probes into cells. This work reports a microfluidic electroporation platform capable of performing multiple molecule delivery to mammalian cells with precise and molecular-dependent parameter control. The system's ability ...
Marinova Zoya Z University Clinics for Child and Adolescent Psychiatry, University of Zürich; - - 2014
Many brain-related disorders have neuronal cell death involved in their pathophysiology. Improved in vitro models to study neuroprotective or neurotoxic effects of drugs and downstream pathways involved would help gain insight into the molecular mechanisms of neuroprotection/neurotoxicity and could potentially facilitate drug development. However, many existing in vitro toxicity assays ...
O'Brien Jason M JM Environmental Health Science and Research Bureau, Health Canada, Environmental Health - - 2014
De novo mutations arise mostly in the male germline and may contribute to adverse health outcomes in subsequent generations. Traditional methods for assessing the induction of germ cell mutations require the use of large numbers of animals, making them impractical. As such, germ cell mutagenicity is rarely assessed during chemical ...
Newell Evan W EW Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), 8A Biomedical Grove, Immunos #03-06, Singapore, 138648, Singapore, - - 2014
Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, ...
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