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Jung Yong-Gyun YG Quantamatrix Inc, 104-213, Seoul National University, Seoul 151-742, Republic of - - 2014
A variety of systems have been developed to study biofilm formation. However, most of the systems are based on the surface-attached growth of microbes under shear stress. In this study, we designed a microfluidic channel device, called an MAC (microfluidic agarose channel), and found that microbial cells in the MAC ...
Shannahan Jonathan H JH * Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045, - - 2014
Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming ...
Madeo Marianna M Sanford Research, United - - 2014
SV2A is a synaptic vesicle membrane protein expressed in neurons and endocrine cells and involved in the regulation of neurotransmitter release. Although the exact function of SV2A still remains elusive, it was identified as the specific binding site for levetiracetam, a second generation antiepileptic drug. Our sequence analysis demonstrates that ...
Chen Jin J Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. - - 2014
The aim of the present study was to evaluate the association between DNA methylation and multidrug resistance (MDR) in glioma and identify novel effectors responsible for MDR in human gliomas. An MDR glioma cell line, SGH‑44/ADM, was developed using adriamycin (ADM) impulse treatment. Cryopreservation, recovery and withdrawal were performed to ...
Wang Huan H Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Heping, Shenyang, Liaoning 110004, - - 2014
Available evidence on the proliferation-promoting effect of HE4 remains controversial, and few studies have been carried out on the molecular mechanism of chemoresistance mediated by HE4. The aim of the present study was to investigate the influence of exogenous recombinant HE4 protein on proliferation and resistance to carboplatin in ovarian ...
Li Yin Y Laboratory of Reproductive and Developmental Toxicology - - 2014
Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines in circulation, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. ...
Sang Xiao-Jing XJ Key Laboratory of Polyoxometalate Science of Ministry of Education, Department of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China. wangeb889@nenu.edu.cn - - 2014
Two novel open-chain carboxyethyltin decorated sandwich-type germanotungstates have been successfully synthesized. They could markedly increase the electrocatalytic activity of single-walled carbon nanotubes toward triiodide reduction when assembled into composite electrodes, which have shown a conversion efficiency of 6.32% that is comparable to that of Pt electrodes (6.29%) when used as ...
Berger Gregory G Department of Infectious Diseases, Faculty of Life Sciences and Medicine, King's College London, Guy's Hospital, London SE1 - - 2014
The accessory gene vpr, common to all primate lentiviruses, induces a potent G2/M arrest in cycling cells. A recent study showed that HIV-1 Vpr mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi Anemia DNA repair pathway. To confirm these observations, we have examined ...
Ortogero Nicole N University of Nevada, Reno, United - - 2014
PIWI-interacting RNAs (piRNAs) are small noncoding RNAs that bind PIWI family proteins exclusively expressed in the germ cells of mammalian gonads. MIWI2-associated piRNAs are essential for silencing transposons during primordial germ cell development, and MIWI-bound piRNAs are required for normal spermatogenesis during adulthood in mice. Although piRNAs have long been ...
Hwang Kevin K Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 - - 2014
DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, ...
Xu Xun X Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustraße 3, 14195, Berlin, - - 2014
Directing stem cells towards a desired location and function by utilizing the structural cues of biomaterials is a promising approach for inducing effective tissue regeneration. Here, the cellular response of human adipose-derived mesenchymal stem cells (hADSCs) to structural signals from microstructured substrates comprising arrays of square-shaped or round-shaped microwells is ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, Council of Science and Industrial Research, Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007 (India); Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad-500 037 (India). - - 2014
A series of chalcone conjugates featuring the imidazo[2,1-b]thiazole scaffold was designed, synthesized, and evaluated for their cytotoxic activity against five human cancer cell lines (MCF-7, A549, HeLa, DU-145 and HT-29). These new hybrid molecules have shown promising cytotoxic activity with IC50 values ranging from 0.64 to 30.9 μM. Among them, (E)-3-(6-(4-fluorophenyl)-2,3-bis(4-methoxyphenyl)imidazo[2,1-b]thiazol-5-yl)-1-(pyridin-2-yl)prop-2-en-1-one ...
Zhang Shaolian S Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. typh@jnu.edu.cn - - 2014
A highly sensitive and recyclable quartz crystal microbalance (QCM) biosensor was developed using chitosan (CS) and folic acid (FA), generating conjugates that are selectively recognized by MCF-7 cancer cell over-expressed folic acid receptors. The prepared CS-FA conjugate was characterized by UV-vis spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy ...
Gallagher Kimberly L KL Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104; email: gallagkl@sas.upenn.edu , - - 2014
Development in multicellular organisms requires the coordinated production of a large number of specialized cell types through sophisticated signaling mechanisms. Non-cell-autonomous signals are one of the key mechanisms by which organisms coordinate development. In plants, intercellular movement of transcription factors and other mobile signals, such as hormones and peptides, is ...
Becker Yvonne Y Massey University, Institute of Fundamental Sciences, Palmerston North, New Zealand ; - - 2014
Epichloё festucae is a mutualistic symbiont that systemically colonises the intercellular spaces of Lolium perenne leaves to form a highly structured and interconnected hyphal network. In an Agrobacterium tumefaciens T-DNA forward genetic screen we identified a mutant TM1066 that had a severe host interaction phenotype, causing stunting and premature senescence ...
Havrylov Serhiy S Goodman Cancer Research Centre, McGill University, H3A1A3, Montreal, QC, Canada; Department of Medicine, McGill University, H3A1A3, Montreal, QC, - - 2014
Acquired capacity of cancer cells to penetrate through the extracellular matrix (ECM) of surrounding tissues is a prerequisite for tumour metastatic spread - the main source of cancer-associated mortality. Through combined efforts of many research groups we are beginning to understand that the ability of cells to invade through the ...
Waki Michihiko M Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, - - 2014
Lipids comprise the primary component of cell membranes. Imaging mass spectrometry is increasingly being used to visualize membranous lipids in clinical specimens, and it has revealed that abnormal lipid metabolism is related to the development of diseases. To characterize cell populations which are rare and sparsely localized in tissues, we ...
Belyaev Victor V - - 2014
The phase retardation difference, ΔΦ, is calculated for hybrid liquid crystal (LC) cells as a function of LC pretilt angles, θ0(1), θ0(2), on the opposite substrates of the cell for the case of an arbitrary angle of light incidence in the range from 0 to 90°. An LC director configuration ...
Goswami Dibakar - - 2014
Iron overload causes progressive and sometimes irreversible damages due to accelerated production of reactive oxygen species. Desferrioxamine (DFO), a siderophore, has been used clinically to remove excess iron. However, the applications of DFO are limited because of its inability to access intracellular pools of labile iron. Cell penetrating peptides (CPPs) ...
Schomaker Markus M Biomedical Optics Department, Laser Zentrum Hannover e. V., Hollerithallee 8, 30419, Hannover, Germany. - - 2014
Gold nanoparticle mediated (GNOME) laser transfection is a powerful technique to deliver small biologically relevant molecules into cells. However, the transfection of larger and especially negatively charged DNA remains challenging. The efficiency for pDNA was 0.57% using parameter that does not influence the endo- and exogenous DNA. In order to ...
Occhetta Paola P Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, - - 2014
The ability to replicate in vitro the native extracellular matrix (ECM) features and to control the three-dimensional (3D) cell organization plays a fundamental role in obtaining functional engineered bio-constructs. In tissue engineering (TE) applications, hydrogels have been successfully implied as biomatrices for 3D cell embedding, exhibiting high similarities to the ...
Gough Mallory M Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YQ, U.K.. Electronic address: - - 2014
Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of ...
Oh Seung Soo SS Materials Department, ‡Department of Chemistry and Biochemistry, §Department of Mechanical Engineering, ∥Materials Research Laboratory, University of California , Santa Barbara, California 93106, United - - 2014
Viruses have evolved specialized mechanisms to efficiently transport nucleic acids and other biomolecules into specific host cells. They achieve this by performing a coordinated series of complex functions, resulting in delivery that is far more efficient than existing synthetic delivery mechanisms. Inspired by these natural systems, we describe a process ...
Fontana Gianluca - - 2014
Intradiscal injection of growth factors or cells has been shown to attenuate symptoms of intervertebral disc degeneration. However, different approaches are needed to overcome limitations such as short-term efficacy and leakage of the injected solutions. The current study aims at creating a platform for the realization of functional cell factories ...
Kawikova Ivana I Section of Clinical Immunology, Yale University School of Medicine, TAC S-217, 300 Cedar St., New Haven, CT 06511, USA. Electronic address: - - 2014
A newly discovered cell-to-cell communication system involves small, membrane-enveloped nanovesicles, called exosomes. We describe here how these extracellular nanoparticles were discovered and how it became gradually apparent that they play fundamental roles in regulation of physiological functions and pathological processes. Exosomes enable intercellular communication by transporting genetic material, proteins and ...
Safarik Ivo I Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Ceske Budejovice, Czech - - 2014
Magnetically modified yeast cells represent an interesting type of biocomposite materials, applicable in various areas of bioanalysis, biotechnology and environmental technology. In this review, typical examples of magnetic modifications of yeast cells of the Saccharomyces, Kluyveromyces, Rhodotorula and Yarrowia genera are presented, as well as their possible applications as biocatalysts, ...
Simasi Jacinta J Fraunhofer-Institut für Zelltherapie und Immunologie, Leipzig, Germany; Institut für Pharmazie, Universität Leipzig, Leipzig, Germany. Electronic address: - - 2014
Tyrosine kinase inhibitors (TKI), erlotinib and gefitinib are small molecule inhibitors which are used for the treatment of lung cancer. But, the development of drug resistance has been reported as one of the major setbacks in oncology. This study focused on the mechanisms leading to secondary resistance by assessing the ...
Balaish Moran M The Nancy & Stephen Grand Technion Energy Program, Technion-Israel Institute of Technology, Haifa, 3200003 - - 2014
Non-aqueous lithium-oxygen batteries are considered as most advanced power sources, albeit they are facing numerous challenges concerning almost each cell component. Herein, we diverge from the conventional and traditional liquid-based non-aqueous Li-O2 batteries to a Li-O2 system based on a solid polymer electrolyte (SPE-) and operated at a temperature higher ...
Lyu Aifeng A Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research and School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China; Jiangsu Hansoh Pharmceutical Corporation, Lianyungang 222000, - - 2014
A series of Lapatinib derivatives were designed and prepared by changing the straight alkyl side chain of Lapatinib into a branched one. ELISA assay and western blot analysis showed that these derivatives can significantly inhibit HER1/HER2 as well as their downstream signal transduction proteins. In vitro cytotoxicity assay revealed that these ...
Guo Xiaoling X Department of Chemistry, Harbin Institute of Technology, Harbin, 150001, China; College of Pharmacy, Jiamusi University, Jiamusi, 154000, - - 2014
Developing a reliable and cost-effective miniaturized electroanalysis tool is of vital importance for cell electrochemical analysis. In this work, a novel mini-electrochemical system has been constructed for trace detection of cell samples. The mini-electrochemical system was constructed by integrating a pencil graphite modified by threonine (PT/PGE) as working electrode, an ...
Gurunathan Sangiliyandi - - 2014
BackgroundGraphene is the 2D form of carbon that exists as a single layer of atoms arranged in a honeycomb lattice and has attracted great interest in the last decade in view of its physical, chemical, electrical, elastic, thermal, and biocompatible properties. The objective of this study was to synthesize an ...
Chatron-Colliet Aurore A UMR CNRS 7369 MEDyC, SFR Cap-Santé, URCA, Reims, - - 2014
During melanoma tumour growth, cancerous cells are exposed to the immediate surrounding the micro- and macro environment, which is largely modified through the degradation of the extracellular matrix by fibroblast-derived metalloproteinases. Among the degradation products, (VGVAPG)3, an elastin peptide is known to stimulate the proliferation of both fibroblasts and cancerous ...
Sharifi Nafiseh N Institute of Nanoscience and Nanotechnology, University of Kashan, Ravand Blv., Kashan 87317 (Iran). - - 2014
The knowledge of dye-sensitized solar cells (DSCs) has expanded considerably in recent years. They are multiparameter and complex systems that work only if various parameters are tuned simultaneously. This makes it difficult to target to a single parameter to improve the efficiency. There is a wealth of knowledge concerning different ...
Whelan D D Centre for Research in Vascular Biology, Biosciences Institute, University College Cork, Cork, - - 2014
Fibrin is formed in the body upon initiation of the clotting cascade and is produced commercially for use as a tissue sealant and hemostasis device during surgical procedures. Experimentally fibrin is being increasingly used as a vector to deliver growth factors, cells, drugs and genes in tissue engineering applications mimicking ...
Zhang Zhuoyuan Z State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China ; Department of Head and Neck Cancer Surgery, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to compare the method of ultracentrifugation and density gradient separation for isolating Tca8113 human tongue squamous cell carcinoma cell line-derived exosomes. The exosomes were obtained from the culture supernatant of cultured Tca8113 cells, respectively, followed by identification with transmission electron microscopy observation and ...
Yoshida Eiko E Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, - - 2014
Methylmercury (MeHg) reacts readily with GSH, leading to the formation of a MeHg-SG adduct that is excreted into extracellular space through multidrug-resistance-associated protein (MRP), which is regulated by the transcription factor Nrf2. We previously reported that MeHg covalently modifies Keap1 and activates Nrf2 in human neuroblastoma SH-SY5Y cells. In the ...
Mueller Larissa L BAM Federal Institute for Materials Research and Testing, 12200, Berlin, Germany, - - 2014
The analysis of single cells is a growing research field in many disciplines such as toxicology, medical diagnosis, drug and cancer research or metallomics, and different methods based on microscopic, mass spectrometric, and spectroscopic techniques are under investigation. This review focuses on the most recent trends in which inductively coupled ...
Meral Ogunc O Faculty of Veterinary Medicine, Department of Biochemistry, Ankara University, 06110, Ankara, - - 2014
Comparative proteomic analysis of normal and cancer cell lines provides for a better understanding of the molecular mechanism of cancer development and is essential for developing more effective strategies for new biomarker or drug target discovery. The purpose of this study is to compare protein expression levels between fibrosarcoma and ...
Arzuman Laila L Discipline of Biomedical Science, Sydney Medical School, The University of Sydney, Lidcombe, NSW, - - 2014
In the present study, synergism in activity from the sequenced combinations of monofunctional platinum tris(benzimidazole)monochloroplatinum(II) chloride (coded as LH4) with capsaicin, quercetin, curcumin and cisplatin was investigated as a function of sequence of administration in a number of human ovarian tumor models. Cellular accumulations of platinum and the levels of ...
Nishida Hiroki H 1 Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, - - 2014
Comparison of features of the cell lineages and fate maps of early embryos between related species is useful in inferring developmental mechanisms and amenable to evolutionary considerations. We present cleavage patterns, cell lineage trees, and fate maps of ascidian and appendicularian embryos side by side to facilitate comparison. This revealed ...
Heller Philipp P Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, D-55128, Mainz, - - 2014
Front Cover: A series of well-defined polypeptide-polypeptoid block copolymers based on the body's own amino acids sarcosine and lysine are prepared by K. Fischer, M. Bros, M. Barz, and co-workers on page 1380 by ring opening polymerization of N-carboxyanhydrides. The obtained block ionomers are used to complex pDNA resulting in ...
Tiedemann Hendrik B HB Institute of Experimental Genetics, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, - - 2014
While it is known that a large fraction of vertebrate genes are under the control of a gene regulatory network (GRN) forming a clock with circadian periodicity, shorter period oscillatory genes like the Hairy-enhancer-of split (Hes) genes are discussed mostly in connection with the embryonic process of somitogenesis. They form ...
Kist Ralf R Institute of Genetic Medicine, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom; Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, United - - 2014
In mammals, taste buds develop in different regions of the oral cavity. Small epithelial protrusions form fungiform papillae on the ectoderm-derived dorsum of the tongue and contain one or few taste buds, while taste buds in the soft palate develop without distinct papilla structures. In contrast, the endoderm-derived circumvallate and ...
Bertaux François F INRIA Paris-Rocquencourt, Le Chesnay, - - 2014
Isogenic cells sensing identical external signals can take markedly different decisions. Such decisions often correlate with pre-existing cell-to-cell differences in protein levels. When not neglected in signal transduction models, these differences are accounted for in a static manner, by assuming randomly distributed initial protein levels. However, this approach ignores the ...
Rehberg Markus M Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, - - 2014
Due to its vital importance in the supply of cellular pathways with energy and precursors, glycolysis has been studied for several decades regarding its capacity and regulation. For a systems-level understanding of the Madin-Darby canine kidney (MDCK) cell metabolism, we couple a segregated cell growth model published earlier with a ...
Abdel Rahman Anas M AM Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Room #988, Toronto, ON, Canada - - 2014
Nutrient transporters are critical gate-keepers of extracellular metabolite entry into the cell. As integral membrane proteins, most transporters are N-glycosylated, and the N-glycans are remodeled in the Golgi apparatus. The Golgi branching enzymes N-acetylglucosaminyltransferases I, II, IV, V and avian VI (encoded by Mgat1, Mgat2, Mgat4a/b/c Mgat5 and Mgat6), each ...
Salehi-Reyhani Ali A Proxomics, Institute of Chemical Biology, Imperial College London, London, SW7 2AZ UK. - - 2014
Addressable droplet microarrays are potentially attractive as a way to achieve miniaturised, reduced volume, high sensitivity analyses without the need to fabricate microfluidic devices or small volume chambers. We report a practical method for producing oil-encapsulated addressable droplet microarrays which can be used for such analyses. To demonstrate their utility, ...
Melo André Luiz de Almeida AL Programa de Pós Graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná , Curitiba, Paraná , - - 2014
Abstract Since the first report by Ishiwata in 1902 of a Bombyx mori infection, followed by the description by Berliner, Bacillus thuringiensis (Bt) has become the main microorganism used in biological control. The application of Bt to combat invertebrates of human interest gained momentum with the growing demand for food ...
Yan Yong Y 1] Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA [2] Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, - - 2014
A concentration cell is composed of two equivalent half-cells made of the same material but differing in the concentration of reactants. As these concentrations equilibrate, the increase in entropy is converted into a flow of electricity with the voltage output determined by the Nernst equation and proportional to the logarithm ...
Osada Naoki N Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, - - 2014
Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals. Vero cells are susceptible to various types of microbes and toxins and have widely contributed to not only ...
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