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Vanajothi Ramar R Department of Bioinformatics, Alagappa University , Karaikudi, Tamilnadu , - - 2014
Abstract Luffa acutangula (Cucurbitaceae) is widely used as a traditional medicine in India and was reported to possess various pharmacological activities including its anti-proliferative effects. In this study, the bioactive compound of ethanolic extract of L. acutangula (LA) was isolated using bioassay-guided approach. Five major fractions were collected and evaluated ...
Burger R R Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Ireland. - - 2014
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene ...
Sharma Archana A Department of Biological Sciences, Birla Institute of Technology and Science, Pilani-K.K Birla Goa Campus, 403726 Goa, - - 2015
Three dimensional scaffolds synthesized using natural or synthetic polymers act as an artificial niche for cell adherence and proliferation. In this study, we have fabricated cryogels employing blend of poly (ethylene glycol) (PEG) and gelatin using two different crosslinkers like, glutaraldehyde and EDC-NHS by cryogelation technique. Synthesized matrices possessed interconnected ...
Yao Peina P Department of chemical engineering, Sichuan University, Chengdu, 610065, P. R. - - 2014
Acetone-butanol-ethanol (ABE) fermentation was studied with Clostridium acetobutylicum as the microorganism in the continuous and closed-circulating fermentation system (CCCF). The experiment lasting for 192 hours was carried out with fermentation integrated with situ pervaporation. In the entire process, the cell growth profile appeared two phases, meaning the logarithmic phase during ...
Sampson Kathleen E KE - - 2014
Membrane transporters P-glycoprotein (P-gp, MDR1 gene), MRP2, and BCRP impact drug absorption and disposition and can also mediate drug-drug interactions leading to safety/toxicity concerns in the clinic. Challenges arise with interpreting cell-based transporter assays when substrates or inhibitors impact more than one actively expressed transporter and when endogenous or residual ...
Ilin Yelena Y Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, - - 2014
Identifying the matrix properties that permit directing stem cell fate is crucial for expanding desired cell lineages ex vivo for disease treatment. Such efforts require knowledge of matrix surface chemistry and the cell responses they elicit. Recent progress in analyzing biomaterial composition and identifying cell phenotype with two label-free chemical ...
Novo Luis - - 2014
The applicability of small interfering RNA (siRNA) in future therapies depends on the availability of safe and efficient carrier systems. Ideally, siRNA delivery requires a system that is stable in the circulation, but upon specific uptake into target cells can rapidly release its cargo into the cytoplasm. Previously, we evaluated ...
Bui Long M G LM Research Centre for Infectious Disease, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, - - 2014
An undetermined feature of Staphylococcus aureus pathogenesis is its persistence then relapse of disease. This has been explained by its switch to alternative lifestyles; mainly as biofilm or Small Colony Variants (SCVs). Studying the native characteristics of SCVs has been problematic due to their reversion to the parental lifestyle. We ...
Hsu T-H TH 1] Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC [2] Genomics Research Center, Academia Sinica, Taipei, Taiwan, - - 2014
The Wnt/β-catenin signaling pathway has emerged as a key regulator of complex biological processes, such as embryonic development, cell proliferation, cell fate decision and tumorigenesis. Recent studies have shown that the deregulation of Wnt/β-catenin signaling is frequently observed and leads to abnormal cell growth in human breast cancer cells. In ...
Qiu Xiaolong X Department of Biomedical Engineering, University of California Irvine, 3107 Natural Sciences II, Irvine, CA 92697, USA. - - 2014
Tumors tissues house a diverse array of cell types, requiring powerful cell-based analysis methods to characterize cellular heterogeneity and identify rare cells. Tumor tissue is dissociated into single cells by treatment with proteolytic enzymes, followed by mechanical disruption using vortexing or pipetting. These procedures can be incomplete and require significant ...
Kruger A M AM Cytopathology, SA Pathology, Adelaide, SA, - - 2014
To compare the Cellient(TM) automated cell block system with the agar cell block method in terms of quantity and quality of diagnostic material and morphological, histochemical and immunocytochemical features. Cell blocks were prepared from 100 effusion samples using the agar method and Cellient system, and routinely sectioned and stained for ...
Mohapatra Jajati K JK Project Directorate on Foot and Mouth Disease, IVRI Campus, India; - - 2014
In this study we describe the adaptive changes fixed on the capsid of several foot-and-mouth disease virus serotype A strains during propagation in cell monolayers. Viruses passaged extensively in three cell lines (BHK-21, LFBK and IB-RS-2), consistently gained positively charged amino acids in the putative heparan sulfate-binding pocket (VP2 βE-βF ...
Lin Linxue L Shandong Liaocheng City Second People's Hospital, Taishan Medical University, Shandong Province 252601, People's Republic of - - 2014
Silk fibroin (SF)/nano-hydroxyapatite (n-HA) composites are potential biomaterials for bone defect repair. Up to now, the biological evaluation studies of SF/n-HA composites have primarily concentrated on their biocompatibility at cell level such as cell viability and proliferation and tissue level such as material absorption and new bone formation. In this ...
Demasi Marilene M Laboratory of Biochemistry and Biophysics, Instituto Butantan, São Paulo, SP, Brazil. Electronic address: - - 2014
Embryogenesis and stem cell differentiation are complex and orchestrated signaling processes. Reactive oxygen species (ROS) act as essential signal transducers in cellular differentiation, as has been shown through recent discoveries. On the other hand, the ubiquitin-proteasome system (UPS) has long been known to play an important role in all cellular ...
Yamaguchi Katsumi K Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-15 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, - - 2014
To elucidate the molecular mechanism of the integration of long interspersed elements (LINEs), we characterized the 5' ends of more than 200 LINE de novo retrotransposition events into chicken DT40 or human HeLa cells. Human L1 inserts produced 15-bp target-site duplications (TSDs) and zebrafish ZfL2-1 inserts produced 5-bp TSDs in ...
Reis Mariana A MA Instituto de Investigação do Medicamento (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Lisbon, - - 2014
Four new diterpenes were isolated from the methanolic extract of Euphorbia piscatoria, two ent-abietanes (1, 2) and two lathyrane-type macrocyclic diterpenes (3, 4), along with three known diterpenes (5-7). Their structures were characterized by spectroscopic methods, mainly 1D and 2D NMR ((1)H, (13)C, DEPT, COSY, HMBC, HMQC, and NOESY) experiments. Compound ...
Wang Li L Central South University, - - 2014
Our previous study demonstrated that the NGX6b gene acts as a suppressor in the invasion and migration of nasopharyngeal carcinoma (NPC). Recently we identified the novel isoform NGX6a which is longer than NGX6b. In this study, we firstly found that NGX6a was degraded in NPC cells and that this degradation ...
Santos Alberto A Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, - - 2014
The eukaryotic cell division cycle is a highly regulated process that consists of a complex series of events and involves thousands of proteins. Researchers have studied the regulation of the cell cycle in several organisms, employing a wide range of high-throughput technologies, such as microarray-based mRNA expression profiling and quantitative ...
Radošević Kristina K Laboratory for Cell Technology, Application and Biotransformations, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, - - 2014
Deep eutectic solvents (DESs) have been dramatically expanding in popularity as a new generation of environmentally friendly solvents with possible applications in various industrial fields, but their ecological footprint has not yet been thoroughly investigated. In the present study, three choline chloride-based DESs with glucose, glycerol and oxalic acid as ...
Watkins Andrew M AM Developmental Toxicology Branch, Toxicity Assessment Division, National Health and Environmental Effects Research Lab, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27709. Electronic address: - - 2014
The 3T3-L1 preadipocyte culture system has been used to examine numerous compounds that influence adipocyte differentiation or function. The perfluoroalkyl acids (PFAAs), used as surfactants in a variety of industrial applications, are of concern as environmental contaminants that are detected worldwide in human serum and animal tissues. This study was ...
Khalil Hilal S HS Scottish Informatics, Mathematics, Biology and Statistics Centre (SIMBIOS), University of Abertay Dundee, Dundee DD1 1HG, United Kingdom. Electronic address: - - 2014
Cells are constantly exposed to Reactive Oxygen Species (ROS) produced both endogenously to meet physiological requirements and from exogenous sources. While endogenous ROS are considered as important signalling molecules, high uncontrollable ROS are detrimental. It is unclear how cells can achieve a balance between maintaining physiological redox homeostasis and robustly ...
Lee Hankeun H Bioelectronics & Microsystems Laboratory, Department of Electrical & Computer Engineering, State University of New York-Binghamton, Binghamton, NY 13902, USA. - - 2014
Self-sustainable energy sources are essential for a wide array of wireless applications deployed in remote field locations. Due to their self-assembling and self-repairing properties, "biological solar (bio-solar) cells" are recently gaining attention for those applications. The bio-solar cell can continuously generate electricity from microbial photosynthetic and respiratory activities under day-night ...
Zhao Peng P Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, - - 2014
Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and their usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional fractionation followed by LC-MS/MS analysis of the ...
Neudorf Kara D KD Department of Biology, University of Regina, - - 2014
Rhizobium leguminosarum is a soil bacterium that is an intracellular symbiont of leguminous plants through the formation of nitrogen-fixing root nodules. Due to the changing environments that rhizobia encounter, the cell is often faced with a variety of cell altering stressors that can compromise the cell envelope integrity. A previously ...
Li Yilin Y School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. - - 2014
A rational design strategy of novel fluorophores for luminescent down-shifting (LDS) application was proposed and tested in this paper. Three new fluorophores () with specific intramolecular charge transfer (ICT) and aggregation-induced emission (AIE) characteristics were synthesized as LDS molecules for increasing the output short circuit current density (Jsc) of a ...
Kurushima Jun J Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi-shi, Gunma 371-8511, - - 2014
Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis, and consists of two extracellular proteins BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids; 64.5 kDa) was a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase inducing cell lysis of E. faecalis when an accessory factor BacA is co-present. ...
Dahan Arik - - 2014
The efficacy of chemotherapeutic drugs is often offset by severe side effects attributable to poor selectivity and toxicity to normal cells. Recently, the enzyme dipeptidyl peptidase IV (DPPIV) was considered as a potential target for the delivery of chemotherapeutic drugs. The purpose of this study was to investigate the feasibility ...
Li Hongli H Department of Ultrasound, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. - - 2014
The present study was designed to determine the efficiency and safety of ultrasound (US) and/or US contrast agent microbubbles (MBs) in the delivery of type 2 recombinant adeno-associated virus‑delivered transforming growth factor‑β2 short hairpin ribonucleic acid encoding the enhanced green fluorescent protein gene (rAAV2‑TGFβ2 shRNA‑EGFP) and the downregulation of TGFβ2 in ...
Wang Qing Q State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, College of Biology, Hunan University, Changsha, 410082, - - 2014
Because cell-specific aptamers have high potential for biomedical applications, investigation of the interaction between cell and its aptamers may be of key importance for an improved understanding of biochemical processes. Herein, the interaction between human lung adenocarcinoma A549 cell and its four aptamers was explored using single-molecule force spectroscopy (SMFS). ...
Chazal Richard - - 2014
Lignins and their cross-linking to hemicelluloses detrimentally affect the cellulose-to-ethanol conversion of grass lignocelluloses. Screening appropriate grass cell walls and their compositional changes during the various steps of the process calls for a high throughput analytical technique. Such a performance can be fulfilled by Fourier transform mid-infrared (FT-MIR) spectroscopy. In ...
van den Brink Susanne C SC Department of Genetics, University of Cambridge, Cambridge CB2 3EH, - - 2014
Mouse embryonic stem cells (mESCs) are clonal populations derived from preimplantation mouse embryos that can be propagated in vitro and, when placed into blastocysts, contribute to all tissues of the embryo and integrate into the normal morphogenetic processes, i.e. they are pluripotent. However, although they can be steered to differentiate ...
Lin Hsing-Ying HY Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, Chiayi, 62102, - - 2014
On-line multiplex SERS detection of pathogenic bacteria is demonstrated at a single-cell level. On page 4700, L.-K. Chau, C.-Y. Yang, and co-workers prepare SERS nanoprobes via immobilization of antibodies on silica-coated, dye-induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well-defined plasmonic hot ...
Kassis Timothy T Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America; School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of - - 2014
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elephantiasis or hydrocele. The mechanisms driving worm migration and retention ...
Bellido Alberto A Departamento de Ciencias Biomédicas, Área de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, - - 2014
rad52-ΔΔ and, to a lesser extent, rad51-ΔΔ deletants of Candidaalbicans displayed slow growth and aberrant filamentous morphology whereas rad59-ΔΔ mutants, both by growth rate and morphology resembled wild type. In this study, we have constructed pair-wise double deletants to analyze genetic interactions among these homologous recombination (HR) proteins that affect ...
Shabram Paul - - 2014
In the past three decades, the use of tumorigenic cell substrates has been the topic of five Vaccine and Related Biological Products Advisory Committee (VRBPAC) meetings, including a review of the A549 cell line in September 2012. Over that period of time, major technological advances in biotechnology have improved our ...
Orhon I I 1] INSERM U1151-CNRS UMR 8253, Paris, France [2] Institut Necker Enfants-Malades (INEM), Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, - - 2014
Motile and primary cilia (PC) are microtubule-based structures located at the cell surface of many cell types. Cilia govern cellular functions ranging from motility to integration of mechanical and chemical signaling from the environment. Recent studies highlight the interplay between cilia and autophagy, a conserved cellular process responsible for intracellular ...
Colombi Annachiara A Department of Mathematical Sciences, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Torino, - - 2014
This paper deals with the derivation of a collective model of cell populations out of an individual-based description of the underlying physical particle system. By looking at the spatial distribution of cells in terms of time-evolving measures, rather than at individual cell paths, we obtain an ensemble representation stemming from ...
Beaussart Audrey A Université Catholique de Louvain, Institute of Life Sciences, B-1348 Louvain-la-Neuve, Belgium. - - 2014
The surface of many bacterial pathogens is covered with polysaccharides that play important roles in mediating pathogen-host interactions. In Streptococcus agalactiae, the capsular polysaccharide (CPS) is recognized as a major virulence factor while the group B carbohydrate (GBC) is crucial for peptidoglycan biosynthesis and cell division. Despite the important roles ...
Marelli Benedetto B Department of Mining and Materials Engineering, Faculty of Engineering, McGill University, 3610 University St., Montréal, QC H3A 0C5, Canada. Electronic address: - - 2014
Technological improvements in collagen gel fabrication are highly desirable as they may enable significant advances in the formation of tissue-equivalent biomaterials for regenerative medicine, three-dimensional (3D) in vitro tissue models, and injectable scaffolds for cell and drug delivery applications. Thus, strategies to modulate collagen gel fibrillar density and organization in the ...
Kikuchi Tetsuhiro T Department of Bioscience, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, - - 2014
Organs are often formed by the extension and branching of epithelial tubes. An appropriate termination of epithelial tube extension is important for generating organs of the proper size and morphology. However, the mechanism by which epithelial tubes terminate their extension is mostly unknown. Here we show that the BED-finger domain ...
Kilic Muhammet Samet MS Department of Chemistry, Bulent Ecevit University, 67100 Zonguldak, - - 2015
This study describes the construction of an enzymatic fuel cell comprised of novel gold nanoparticles embedded poly(propylene-co-imidazole) coated anode and cathode. Working electrode fabrication steps and operational conditions for the fuel cell have been optimized to get enhanced power output. Electrical generation capacity of the optimized cell was tested by ...
Haldar Srijan - - 2014
Megakaryocytes exit from mitotic cell cycle and enter a phase of repeated DNA replication without undergoing cell division, in a process termed as endomitosis of which little is known. We studied the expression of a DNA replication licensing factor mini chromosome maintenance protein 7 (MCM7) and its intronic miR-106b-25 cluster ...
Giles Rachel H RH 0000000191332008 Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, the - - 2014
We have developed a novel 3D cell culture model that uses mouse inner-medullary collecting duct (mIMCD3) cells to generate epithelial spheroids. This model is amenable to efficient siRNA knockdown and subsequent rescue with human patient-derived alleles. Spheroids develop apicobasal polarity and complete lumens, and they are consequently an ideal model ...
Le Floc'h Audrey A Immunology Program, Memorial Sloan-Kettering Cancer Center, Box 514, 1275 York Ave, New York, NY, 10065, USA, - - 2014
Transient,specialized cell-cell interactions play a central role in leukocyte function by enabling specific intercellular communication in the context of a highly dynamic systems level response. The dramatic structural changes required for the formation of these contacts are driven by rapid and precise cytoskeletal remodeling events. In recent years, the immunological ...
Oliveira Bruno Rodrigues BR Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande. Av. Italia Km8, Rio Grande, RS, 96203-900, - - 2014
The OCT4 transcription factor is a crucial stem cells marker and it has been related to the cancer stem cells concept. Moreover, it has also been associated to the multiple drug resistance (MDR) phenotype. Our first results pointed out a straight relation between OCT4 and ABC transporters in K562-derivative MDR ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India. - - 2014
A series of imidazo[1,5-a]pyridine-benzimidazole hybrids () were prepared and evaluated for their cytotoxic activity against a panel of sixty human tumor cell lines. Among them compounds and showed significant cytotoxic activity with GI50 values ranging from 1.06 to 14.9 μM and 0.43 to 7.73 μM, respectively. Flow cytometric analysis revealed ...
Mercier Romain R Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United - - 2014
The peptidoglycan cell wall is a defining structural feature of the bacterial-kingdom. Curiously, some bacteria have the ability to switch to a wall-free or 'L-form' state. Although known for decades, the general properties of L-forms are poorly understood, largely due to the lack of their systematic analysis in the molecular ...
Morhayim Jess J Department of Internal Medicine and Erasmus MC Stem Cell and Regenerative Medicine - - 2014
Beyond forming bone, osteoblasts play pivotal roles in various biologic processes, including hematopoiesis and bone metastasis. Extracellular vesicles (EVs) have been implicated in intercellular communication via transfer of proteins and nucleic acids between cells. We focused on the proteomic characterization of nonmineralizing (NMOBs) and mineralizing (MOBs) human osteoblast (SV-HFOs) EVs ...
Balakrishnan Sruthi S SS National Centre for Biological Sciences, TIFR-GKVK Campus, Bellary Road, Bangalore 560065, - - 2014
Phosphoinositides (PtdInsPs) are lipids that mediate a range of conserved cellular processes in eukaryotes. These include the transduction of ligand binding to cell surface receptors, vesicular transport and cytoskeletal function. The nature and functions of PtdInsPs were initially elucidated through biochemical experiments in mammalian cells. However, over the years, genetic ...
Kong Janay Elise JE Department of Bioengineering, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, California 90095, USA. - - 2014
Lab on a chip systems have often focused on diagnostic, chemical, and cell analysis applications, however, more recently the scale and/or precision of micro-engineered systems has been applied in developing new therapies. In this issue we highlight recent work using microfluidic and micro-engineered systems in therapeutic applications. We discuss two ...
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