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Bhattaram Pallavi P Department of Cellular and Molecular Medicine, Orthopaedic and Rheumatologic Research Center, Cleveland Clinic Lerner Research Institute, Cleveland, OH 44195 lefebvv@ccf.org - - 2014
Canonical WNT signaling stabilizes β-catenin to determine cell fate in many processes from development onwards. One of its main roles in skeletogenesis is to antagonize the chondrogenic transcription factor SOX9. We here identify the SOXC proteins as potent amplifiers of this pathway. The SOXC genes, i.e., Sox4, Sox11, and Sox12, ...
Guo Y Z YZ Department of Clinical Sciences, SLU, Uppsala, - - 2014
E. coli is one of the most frequent bacteria involved in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in the pathogenic processes leading to postpartum metritis and endometritis in cattle. It also causes inflammation of the endometrium. Increase of cell proliferation by ...
Congras A A INRA, UMR1388 Genetique, Physiologie et Systemes d'Elevage GenPhySE, Castanet-Tolosan, - - 2014
Chromosomal rearrangements have a crucial impact on the proper proceedings of meiosis and can lead by several mechanisms to the production of unbalanced gametes or to the complete arrest of gametes production. To assess the impact of these rearrangements in the early development of pig germ cells, we proposed to ...
Matsunari H H Meiji University International Institute for Bio-Resource Research (MUIIBR), Kawasaki, - - 2014
Genome editing technologies have been used as a powerful strategy for the generation of genetically modified pigs. We previously developed genetically modified clone pigs with organogenesis-disabled phenotypes, as well as pigs exhibiting diseases with similar features to those of humans. Here, we report the production efficiency of various gene knockout ...
Sugiyama Mayu M Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, Wako-city, Saitama, - - 2014
In multicellular organism development, a stochastic cellular response is observed, even when a population of cells is exposed to the same environmental conditions. Retrieving the spatiotemporal regulatory mode hidden in the heterogeneous cellular behavior is a challenging task. The G1/S transition observed in cell cycle progression is a highly stochastic ...
Pan Chuanying C College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, PR China; Institute for Regenerative Medicine, Wake Forest University, 391 Technology Way, Winston-Salem, NC 27101, - - 2014
Somatic cell reprogramming has generated enormous interest, following the first report of generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts, but the integration of viral transgenes into the genome is unlikely to be accepted. Given these safety considerations, a method for virus-free transient gene expression from suspension-adapted Sf9 ...
Via Allegra A Department of Physics, Sapienza University, 00185 Rome, - - 2014
Molecular mimicry is one of the powerful stratagems that pathogens employ to colonise their hosts and take advantage of host cell functions to guarantee their replication and dissemination. In particular, several viruses have evolved the ability to interact with host cell components through protein short linear motifs (SLiMs) that mimic ...
Cui Yi Y Department of Agricultural and Biological Engineering, Bindley Bioscience Center and Birck Nanotechnology Center, Purdue University, West Lafayette, IN, - - 2014
Single-cell explorations offer a unique window to inspect molecules and events relevant to mechanisms and heterogeneity constituting the central dogma of biology. A large number of nucleic acids, proteins, metabolites, and small molecules are involved in determining and fine-tuning the state and function of a single cell at a given ...
Smeekens Johanna M JM School of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332, - - 2014
Cell surface N-glycoproteins play extraordinarily important roles in cell-cell communication, cell-matrix interactions, and cellular response to environmental cues. Global analysis is exceptionally challenging because many N-glycoproteins are present at low abundances and effective separation is difficult to achieve. Here, we have developed a novel strategy integrating metabolic labeling, copper-free click ...
Guo Shan S Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of - - 2014
Multifunctional nanomaterials simultaneously possessing attractive properties, such as strong fluorescent intensity, excellent superparamagnetic behavior, easy modification and good biocompatibility, are always desired in a wide range of applications. In this work, we present a facile ultrasonication-assisted one-step self-assembly strategy for the fabrication of smart fluorescent-magnetic nanobeads (FMNBs) without using a ...
Fleurie Aurore A Bases Moléculaires et Structurales des Systèmes Infectieux, IBCP, Université Lyon 1, CNRS, UMR 5086, Lyon 69007, - - 2014
In every living organism, cell division requires accurate identification of the division site and placement of the division machinery. In bacteria, this process is traditionally considered to begin with the polymerization of the highly conserved tubulin-like protein FtsZ into a ring that locates precisely at mid-cell. Over the past decades, ...
Furtak Vyacheslav A VA Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD, - - 2014
In this study, we assessed the feasibility of tangential flow filtration (TFF) for primary concentration of viral adventitious agents (AAs) from large volumes of cell substrate-derived samples, such as cell-free Chinese hamster ovary (CHO) culture supernatants (500 mL) and CHO cell lysates (50 mL), prior to virus detection in them by nucleic ...
Besserer Jürgen J Institute of Physics, Science Faculty, University of Zürich, Zürich, Switzerland; Radiotherapy Hirslanden, Witellikerstrasse 40, Zürich, - - 2014
When fractionation schemes for hypofractionation and stereotactic body radiotherapy are considered, a reliable cell survival model at high dose is needed for calculating doses of similar biological effectiveness. In this work a simple model for cell survival which is valid also at high dose is developed from Poisson statistics. An ...
Gaillochet Christophe C Department of Stem Cell Biology, Centre for Organismal Studies, University of Heidelberg, D-69120 Heidelberg, - - 2014
Plants develop postembryonically from pools of continuously active stem cells embedded in specialized tissues called meristems, which are located at the growing points of shoot and root. How these stem cells are established, maintained and guided towards differentiation within the highly dynamic shoot apical meristem is only beginning to emerge. ...
Sedla─Źková Eva E Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute, SAS, Vlarska 7, Bratislava, - - 2014
7H-Dibenzo[c,g]carbazole (DBC) is a heterocyclic aromatic hydrocarbon that is carcinogenic in many species and tissues. DBC is a common environmental pollutant, and is therefore constantly exposed to sunlight. However, there are limited data exploring the toxicity of DBC photoexcitation products. Here, we investigated the impact of ultraviolet (UV) A radiation ...
Milone Maria Rita MR Centro Ricerche Oncologiche Mercogliano, Istituto Nazionale Tumori Fondazione G. Pascale - IRCCS, Naples, - - 2014
Proteomic analysis identified differentially expressed proteins between zoledronic acid-resistant and aggressive DU145R80 prostate cancer (PCa) cells and their parental DU145 cells. Ingenuity Pathway Analysis (IPA) showed a strong relationship between the identified proteins within a network associated with cancer and with homogeneous cellular functions prevalently related with regulation of cell ...
Krüger Christopher T CT Department of Food Chemistry and Toxicology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131, Karlsruhe, - - 2014
The X-linked PIG-A gene is involved in the biosynthesis of the cell surface anchor GPI, and its inactivation may serve as a new marker for mutagenicity. The in vivo PIG-A gene mutation assay is currently being validated by several groups. In this study, we established a corresponding in vitro variant ...
Sijen Titia T Department of Human Biological Traces, Netherlands Forensic Institute, PO Box 24044, 2490 AA The Hague, The Netherlands. Electronic address: - - 2014
Human biological traces have the potential to present strong evidence for placing a suspect at a crime scene. In cases, the activity that led to deposition of an individual's cellular material is increasingly disputed, for which the identification of cell types could be crucial. This review aims to give an ...
Millet Jean Kaoru JK Department of Microbiology and Immunology, Cornell University, C4 127 VMC, Ithaca, NY 14853, United - - 2014
Coronaviruses are a large group of enveloped, single-stranded positive-sense RNA viruses that infect a wide range of avian and mammalian species, including humans. The emergence of deadly human coronaviruses, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle East respiratory syndrome coronavirus (MERS-CoV) have bolstered research in these viral and often ...
Wecksler Aaron T AT Department of Entomology, University of California, Davis, CA, 95616, - - 2014
Sorafenib (Nexavar(®)) is currently the only FDA-approved small molecule targeted therapy for advanced hepatocellular carcinoma. The use of structural analogues and derivatives of sorafenib has enabled the elucidation of critical targets and mechanism(s) of cell death for human cancer lines. We previously performed a structure-activity relationship study on a series ...
Luchinat E E Magnetic Resonance Center - CERM, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy. - - 2014
An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresponding to zinc binding to SOD1 observed by in-cell ...
Li Jiang J Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 - - 2014
A very attractive goal in nanotechnology is to manufacture smart nanodevices that integrate multiple biological/biomedical functions and autonomously function in vivo in a predefined and well-controlled manner. For decades, researchers have been developing many different ways toward this target, using bottom-up assembly of types of nanomaterials or top-down fabrication of ...
Suzuki Atsushi A Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National University, Yokohama, Kanagawa, Japan Department of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Yokohama, Kanagawa, Japan atsuzuki@ynu.ac.jp - - 2014
NANOS2 and NANOS3 belong to the Nanos family of proteins that contain a conserved zinc finger domain, which consists of two consecutive CCHC-type zinc finger motifs, and contribute to germ cell development in mice. Previous studies indicate that there are redundant and distinct functions of these two proteins. NANOS2 rescues ...
Miao Zhengrui Z Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, United - - 2014
A novel fluorescein-based fluorescent probe for nitroxyl (HNO) based on the reductive Staudinger ligation of HNO with an aromatic phosphine was prepared. This probe reacts with HNO derived from Angeli's salt and 4-bromo Piloty's acid under physiological conditions without interference by other biological redox species. Confocal microscopy demonstrates this probe ...
Aleynova O A OA Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, - - 2014
Resveratrol is a naturally occurring plant stilbene that exhibits a wide range of valuable biological and pharmacological properties. Although the beneficial effects of trans-resveratrol to human health and plant protection against fungal pathogens are well-established, little is known about the molecular mechanisms regulating stilbene biosynthesis in plant cells. It has ...
Roopan Selvaraj Mohana SM Chemistry Research Laboratory, Organic Chemistry Division, School of Advanced Sciences, VIT University, Vellore, Tamil Nadu, 632 014, India, - - 2014
In this paper, we have established for the first time, the terrific efficiency of aqueous extract of agricultural waste dried peel of sugar apple (Annona squamosa) in the rapid synthesis of stable SnO2 nanoparticles. In topical years, the deployment of secondary metabolites from plant extract has emerged as a novel ...
Siddiqui Sahabjada - - 2014
BackgroundOsteoporosis is one of the prevalent diseases in ageing populations. Due to side effects of many chemotherapeutic agents, there is always a need to search for herbal products to treat the disorder. Punica granatum (PG) represent a potent fruit-bearing medicinal herb which exerted valuable anti-osteoporotic activities. The present study was ...
Liang Yong Y Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, State Key Lab of Marine Environmental Science, Xiamen University, Xiamen, 361005, - - 2014
We report a novel activity-based and Cu-free click chemistry (CC) mediated methodology for glutathione S-transferase omega 1 (GSTO1) quantification using species-unspecific isotope dilution inductively coupled plasma mass spectrometry (SUID ICP-MS), in which dibenzylcyclooctyne-modified 2-chloroacetamide (DBCO-ChAcA) was designed and synthesized, meanwhile, as a navigator towards GSTO1 for subsequent N3-DOTA-Eu-tagging via Cu-free ...
Brightwell Sara S MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh, EH16 4UU, - - 2014
Generation of iPSCs is inefficient and the molecular mechanisms underlying reprogramming are not well understood. While several studies have demonstrated that reprogramming is not entirely a random process and contains predictable stepwise changes, varying degrees of cellular heterogeneity that arise in different reprogramming systems can obscure the process. Among several ...
Cao Meng - - 2014
Missing in metastasis (MIM, also MTSS1) is a member of the inverse Bin-Amphiphysin-Rvs (I-BAR) family that senses and stabilizes negative membrane protrusions. Abnormal expression of MIM has been frequently associated with a subset of human cancers and may play different roles in different stages of tumor progression. Overexpression of MIM-I-BAR ...
Caputo Emilia E Institute of Genetics and Biophysics -I.G.B. A. Buzzati-Traverso- CNR, Naples I-80131, Italy; Dipartimento di Scienze Biomediche, Università degli Studi di Catania, Catania I-95124, Italy. Electronic address: - - 2014
We performed a comparative study between two human metastatic melanoma cell lines (A375 and 526), and melanocytes (FOM78) by gene expression profiling and pathway analysis, using Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) software. Genes involved in Ran signaling were significantly over-represented (p ≤ 0.001) and up-regulated in melanoma ...
Zhao Bo B Dorris Neuroscience Center, Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, - - 2014
Hair cells are the mechanosensory cells of the inner ear. Mechanotransduction channels in hair cells are gated by tip links. The molecules that connect tip links to transduction channels are not known. Here we show that the transmembrane protein TMIE forms a ternary complex with the tip-link component PCDH15 and ...
Fan Gaofeng - - 2014
Although DNA encodes the molecular instructions that underlie control of cell function, it is the proteins that are primarily responsible for implementing those instructions. Therefore, quantitative analyses of the proteome would be expected to yield insights into important candidates for the detection and treatment of disease. We present an iTRAQ ...
Stratz Simone - - 2014
Individual bacteria of an isogenic population can differ significantly in their phenotypic characteristics. This cellular heterogeneity is thought to increase the adaptivity to environmental changes on a population level. Analytical methods for single-bacteria analyses are essential to reveal the different factors that may contribute to this cellular heterogeneity, among them ...
Cvekl Aleš A Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY 10461, USA ales.cvekl@einstein.yu.edu - - 2014
The ocular lens is a model system for understanding important aspects of embryonic development, such as cell specification and the spatiotemporally controlled formation of a three-dimensional structure. The lens, which is characterized by transparency, refraction and elasticity, is composed of a bulk mass of fiber cells attached to a sheet ...
Ni Yilu Y National Engineering Research Center for Biomaterials, Sichuan University, Wangjiang Road 29, Chengdu 610064, - - 2014
Natural polysaccharides are extensively investigated as cell scaffold materials for cellular adhesion, proliferation, and differentiation due to their excellent biocompatibility, biodegradability, and biofunctions. However, their application is often severely limited by their mechanical behavior. In this study, a tough and elastic hydrogel scaffold was prepared with hyaluronic acid (HA) and ...
Kolisko Martin M Canadian Institute for Advanced Research, Botany Department, University of British Columbia, 3529-6270 University Boulevard, Vancouver, BC, V6T 1Z4, - - 2014
One of the greatest hindrances to a comprehensive understanding of microbial genomics, cell biology, ecology, and evolution is that most microbial life is not in culture. Solutions to this problem have mainly focused on whole-community surveys like metagenomics, but these analyses inevitably loose information and present particular challenges for eukaryotes, ...
Chen Xu X 1] Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria [2] Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie (VIB), Ghent University, B-9052 Gent, Belgium [3] Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Gent, - - 2014
The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating ...
Lopez Renee A RA Department of Plant Biology, Southern Illinois University Carbondale, Carbondale, Illinois 62901 - - 2014
• Sperm cell differentiation in ferns involves the origin of an elaborate locomotory apparatus, including 70+ flagella, and the structural modification of every cellular component. Because arabinogalactan proteins (AGPs) are implicated in molecular signaling and in regulation of plant development, we speculated that these glycoproteins would be present during spermiogenesis ...
Park Ji Hyun JH Cancer Epigenetics Laboratory University of Bristol, Bristol BS8 1TD, - - 2014
Approximately half of poor prognosis neuroblastomas (NBs) are characterized by pathognomonic MYCN gene amplification and MYCN over-expression. Here we present data showing that short-interfering RNA mediated depletion of the protein arginine methyltransferase 5 (PRMT5) in cell-lines representative of NBs with MYCN gene amplification leads to greatly impaired growth and apoptosis. ...
Paulitschke Verena V Dept. of Dermatology, Medical University of Vienna, Vienna, - - 2014
To shed light on the multistep process of squamous cell carcinoma development and the underlying pathologic mechanisms, we performed comparative proteome analysis of keratinocytes, keratinocytes stimulated with Il-1beta and A431 epidermoid carcinoma cells. Fractionation of the cells into supernatant, nucleus and cytoplasm was followed by protein separation, proteolytic digest and ...
Cordeiro Rosemeyre A RA Department of Chemical Engineering, University of Coimbra, Polo II, Rua Sílvio Lima, 3030-790, Coimbra, - - 2014
This manuscript reports the synthesis of a new cationic block copolymer based on poly[2-(dimethylamino)ethyl methacrylate] and poly(β-amino ester) from different polymerization strategies. For the first time, it is proposed a triblock copolymer based only on cationic segments, aiming a high biocompatibility, enhanced buffering capacity and stimuli-responsive character in a single ...
Marciniak Anja A 1] Deutsche Forschungsgemeinschaft (DFG) Research Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany. [2] Paul Langerhans Institute Dresden, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. [3] German Center for Diabetes Research (DZD), Dresden, - - 2014
Studies on the cellular function of the pancreas are typically performed in vitro on its isolated functional units, the endocrine islets of Langerhans and the exocrine acini. However, these approaches are hampered by preparation-induced changes of cell physiology and the lack of an intact surrounding. We present here a detailed ...
Ritchie Kieran K Departments of Paediatrics and Biochemistry, Children's Health Research Institute, University of Western Ontario, Victoria Research Laboratories, 800 Commissioners Road East, London, ON N6C 2V5, - - 2014
Mutations in the alpha-thalassemia mental retardation X-linked (ATRX) gene cause a spectrum of abnormalities including intellectual disability, developmental delay, seizures, and microcephaly. The ATRX protein is highly enriched at heterochromatic repetitive sequences adjacent to the centromere, and ATRX depletion results in chromosome congression, segregation, and cohesion defects. Here, we show ...
Lyons David A DA Centre for Neuroregeneration, University of Edinburgh, Edinburgh EH16 4SB, United - - 2014
The zebrafish is a premier vertebrate model system that offers many experimental advantages for in vivo imaging and genetic studies. This review provides an overview of glial cell types in the central and peripheral nervous system of zebrafish. We highlight some recent work that exploited the strengths of the zebrafish ...
Uziel O O FMRC, RMC, Sackler School of Medicine, Tel Aviv University, Israel. Electronic address: - - 2014
Previously, we have shown that shortening of telomeres by telomerase inhibition sensitized cancer cells to cisplatinum, slowed their migration, increased DNA damage and impaired DNA repair. The mechanism behind these effects is not fully characterized. Its clarification could facilitate novel therapeutics development and may obviate the time consuming process of ...
Casanova Magali M Centre National de la Recherche Scientifique (CNRS), 5290-IRD 224-University Montpellier 1, Research Unit "MIVEGEC", Montpellier, - - 2014
Microtubules are subject to post-translational modifications, which are thought to have crucial roles in the function of complex microtubule-based organelles. Among these, polyglutamylation was relatively recently discovered, and was related to centrosome stability, axonemal maintenance and mobility, and neurite outgrowth. In trypanosomatids, parasitic protozoa where microtubules constitute the essential component ...
Mayya Viveka V Skirball Institute of Biomolecular Medicine, NYU Medical Center, 540 First Avenue, New York, NY 10016, - - 2014
Integrative analytical approaches are needed to study and understand T cell motility as it is a highly coordinated and complex process. Several computational algorithms and tools are available to track motile cells in time-lapse microscopy images. In contrast, there has only been limited effort towards the development of tools that ...
Zhang Xuejiao X Institut für Chemie und Biochemie, Freie Universität Berlin, Takustraße 3, 14195, Berlin, - - 2014
A novel adenosine-5'-triphosphate (ATP) and pH dual-responsive degradable nanogel (NG) system are developed based on the complexation of 1,2-diols in dendritic polyglycerol (dPG), and boronic acids, which are conjugated with dPG as the macromolecular cross-linker. The NG is formed by a mild and surfactant-free inverse nanoprecipitation method. An anticancer drug, ...
Manfrini Marco M Department of Morphology, Surgery and Experimental Medicine, School of Medicine, University of Ferrara, Ferrara, - - 2014
Biomaterials employed for the bone regeneration can be assayed for specific features such as osteoconductivity and gene expression. In this study, the composite HA/collagen/chondroitin-sulfate biomaterial was investigated using an engineered human cell line, named Saos-eGFP. This cell line, a green fluorescent engineered human osteoblast-like cell, was employed as a cellular ...
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