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Yao Peina P Department of chemical engineering, Sichuan University, Chengdu, 610065, P. R. - - 2014
Acetone-butanol-ethanol (ABE) fermentation was studied with Clostridium acetobutylicum as the microorganism in the continuous and closed-circulating fermentation system (CCCF). The experiment lasting for 192 hours was carried out with fermentation integrated with situ pervaporation. In the entire process, the cell growth profile appeared two phases, meaning the logarithmic phase during ...
Sampson Kathleen E KE - - 2014
Membrane transporters P-glycoprotein (P-gp, MDR1 gene), MRP2, and BCRP impact drug absorption and disposition and can also mediate drug-drug interactions leading to safety/toxicity concerns in the clinic. Challenges arise with interpreting cell-based transporter assays when substrates or inhibitors impact more than one actively expressed transporter and when endogenous or residual ...
Novo Luis - - 2014
The applicability of small interfering RNA (siRNA) in future therapies depends on the availability of safe and efficient carrier systems. Ideally, siRNA delivery requires a system that is stable in the circulation, but upon specific uptake into target cells can rapidly release its cargo into the cytoplasm. Previously, we evaluated ...
Bui Long M G LM Research Centre for Infectious Disease, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, - - 2014
An undetermined feature of Staphylococcus aureus pathogenesis is its persistence then relapse of disease. This has been explained by its switch to alternative lifestyles; mainly as biofilm or Small Colony Variants (SCVs). Studying the native characteristics of SCVs has been problematic due to their reversion to the parental lifestyle. We ...
Hsu T-H TH 1] Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC [2] Genomics Research Center, Academia Sinica, Taipei, Taiwan, - - 2014
The Wnt/β-catenin signaling pathway has emerged as a key regulator of complex biological processes, such as embryonic development, cell proliferation, cell fate decision and tumorigenesis. Recent studies have shown that the deregulation of Wnt/β-catenin signaling is frequently observed and leads to abnormal cell growth in human breast cancer cells. In ...
Qiu Xiaolong X Department of Biomedical Engineering, University of California Irvine, 3107 Natural Sciences II, Irvine, CA 92697, USA. - - 2014
Tumors tissues house a diverse array of cell types, requiring powerful cell-based analysis methods to characterize cellular heterogeneity and identify rare cells. Tumor tissue is dissociated into single cells by treatment with proteolytic enzymes, followed by mechanical disruption using vortexing or pipetting. These procedures can be incomplete and require significant ...
Kruger A M AM Cytopathology, SA Pathology, Adelaide, SA, - - 2014
To compare the Cellient(TM) automated cell block system with the agar cell block method in terms of quantity and quality of diagnostic material and morphological, histochemical and immunocytochemical features. Cell blocks were prepared from 100 effusion samples using the agar method and Cellient system, and routinely sectioned and stained for ...
Mohapatra Jajati K JK Project Directorate on Foot and Mouth Disease, IVRI Campus, India; - - 2014
In this study we describe the adaptive changes fixed on the capsid of several foot-and-mouth disease virus serotype A strains during propagation in cell monolayers. Viruses passaged extensively in three cell lines (BHK-21, LFBK and IB-RS-2), consistently gained positively charged amino acids in the putative heparan sulfate-binding pocket (VP2 βE-βF ...
Yamaguchi Katsumi K Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-15 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, - - 2014
To elucidate the molecular mechanism of the integration of long interspersed elements (LINEs), we characterized the 5' ends of more than 200 LINE de novo retrotransposition events into chicken DT40 or human HeLa cells. Human L1 inserts produced 15-bp target-site duplications (TSDs) and zebrafish ZfL2-1 inserts produced 5-bp TSDs in ...
Reis Mariana A MA Instituto de Investigação do Medicamento (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Lisbon, - - 2014
Four new diterpenes were isolated from the methanolic extract of Euphorbia piscatoria, two ent-abietanes (1, 2) and two lathyrane-type macrocyclic diterpenes (3, 4), along with three known diterpenes (5-7). Their structures were characterized by spectroscopic methods, mainly 1D and 2D NMR ((1)H, (13)C, DEPT, COSY, HMBC, HMQC, and NOESY) experiments. Compound ...
Wang Li L Central South University, - - 2014
Our previous study demonstrated that the NGX6b gene acts as a suppressor in the invasion and migration of nasopharyngeal carcinoma (NPC). Recently we identified the novel isoform NGX6a which is longer than NGX6b. In this study, we firstly found that NGX6a was degraded in NPC cells and that this degradation ...
Santos Alberto A Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, - - 2014
The eukaryotic cell division cycle is a highly regulated process that consists of a complex series of events and involves thousands of proteins. Researchers have studied the regulation of the cell cycle in several organisms, employing a wide range of high-throughput technologies, such as microarray-based mRNA expression profiling and quantitative ...
Lee Hankeun H Bioelectronics & Microsystems Laboratory, Department of Electrical & Computer Engineering, State University of New York-Binghamton, Binghamton, NY 13902, USA. - - 2014
Self-sustainable energy sources are essential for a wide array of wireless applications deployed in remote field locations. Due to their self-assembling and self-repairing properties, "biological solar (bio-solar) cells" are recently gaining attention for those applications. The bio-solar cell can continuously generate electricity from microbial photosynthetic and respiratory activities under day-night ...
Zhao Peng P Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, - - 2014
Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and their usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional fractionation followed by LC-MS/MS analysis of the ...
Neudorf Kara D KD Department of Biology, University of Regina, - - 2014
Rhizobium leguminosarum is a soil bacterium that is an intracellular symbiont of leguminous plants through the formation of nitrogen-fixing root nodules. Due to the changing environments that rhizobia encounter, the cell is often faced with a variety of cell altering stressors that can compromise the cell envelope integrity. A previously ...
Li Yilin Y School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. - - 2014
A rational design strategy of novel fluorophores for luminescent down-shifting (LDS) application was proposed and tested in this paper. Three new fluorophores () with specific intramolecular charge transfer (ICT) and aggregation-induced emission (AIE) characteristics were synthesized as LDS molecules for increasing the output short circuit current density (Jsc) of a ...
Kurushima Jun J Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi-shi, Gunma 371-8511, - - 2014
Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis, and consists of two extracellular proteins BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids; 64.5 kDa) was a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase inducing cell lysis of E. faecalis when an accessory factor BacA is co-present. ...
Dahan Arik - - 2014
The efficacy of chemotherapeutic drugs is often offset by severe side effects attributable to poor selectivity and toxicity to normal cells. Recently, the enzyme dipeptidyl peptidase IV (DPPIV) was considered as a potential target for the delivery of chemotherapeutic drugs. The purpose of this study was to investigate the feasibility ...
Li Hongli H Department of Ultrasound, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. - - 2014
The present study was designed to determine the efficiency and safety of ultrasound (US) and/or US contrast agent microbubbles (MBs) in the delivery of type 2 recombinant adeno-associated virus‑delivered transforming growth factor‑β2 short hairpin ribonucleic acid encoding the enhanced green fluorescent protein gene (rAAV2‑TGFβ2 shRNA‑EGFP) and the downregulation of TGFβ2 in ...
Wang Qing Q State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, College of Biology, Hunan University, Changsha, 410082, - - 2014
Because cell-specific aptamers have high potential for biomedical applications, investigation of the interaction between cell and its aptamers may be of key importance for an improved understanding of biochemical processes. Herein, the interaction between human lung adenocarcinoma A549 cell and its four aptamers was explored using single-molecule force spectroscopy (SMFS). ...
Chazal Richard - - 2014
Lignins and their cross-linking to hemicelluloses detrimentally affect the cellulose-to-ethanol conversion of grass lignocelluloses. Screening appropriate grass cell walls and their compositional changes during the various steps of the process calls for a high throughput analytical technique. Such a performance can be fulfilled by Fourier transform mid-infrared (FT-MIR) spectroscopy. In ...
van den Brink Susanne C SC Department of Genetics, University of Cambridge, Cambridge CB2 3EH, - - 2014
Mouse embryonic stem cells (mESCs) are clonal populations derived from preimplantation mouse embryos that can be propagated in vitro and, when placed into blastocysts, contribute to all tissues of the embryo and integrate into the normal morphogenetic processes, i.e. they are pluripotent. However, although they can be steered to differentiate ...
Lin Hsing-Ying HY Department of Chemistry and Biochemistry and Center for Nano Bio-Detection (AIM-HI), National Chung Cheng University, Chiayi, 62102, - - 2014
On-line multiplex SERS detection of pathogenic bacteria is demonstrated at a single-cell level. On page 4700, L.-K. Chau, C.-Y. Yang, and co-workers prepare SERS nanoprobes via immobilization of antibodies on silica-coated, dye-induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well-defined plasmonic hot ...
Kassis Timothy T Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America; School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of - - 2014
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elephantiasis or hydrocele. The mechanisms driving worm migration and retention ...
Orhon I I 1] INSERM U1151-CNRS UMR 8253, Paris, France [2] Institut Necker Enfants-Malades (INEM), Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, - - 2014
Motile and primary cilia (PC) are microtubule-based structures located at the cell surface of many cell types. Cilia govern cellular functions ranging from motility to integration of mechanical and chemical signaling from the environment. Recent studies highlight the interplay between cilia and autophagy, a conserved cellular process responsible for intracellular ...
Colombi Annachiara A Department of Mathematical Sciences, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129, Torino, - - 2014
This paper deals with the derivation of a collective model of cell populations out of an individual-based description of the underlying physical particle system. By looking at the spatial distribution of cells in terms of time-evolving measures, rather than at individual cell paths, we obtain an ensemble representation stemming from ...
Beaussart Audrey A Université Catholique de Louvain, Institute of Life Sciences, B-1348 Louvain-la-Neuve, Belgium. - - 2014
The surface of many bacterial pathogens is covered with polysaccharides that play important roles in mediating pathogen-host interactions. In Streptococcus agalactiae, the capsular polysaccharide (CPS) is recognized as a major virulence factor while the group B carbohydrate (GBC) is crucial for peptidoglycan biosynthesis and cell division. Despite the important roles ...
Giles Rachel H RH 0000000191332008 Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, the - - 2014
We have developed a novel 3D cell culture model that uses mouse inner-medullary collecting duct (mIMCD3) cells to generate epithelial spheroids. This model is amenable to efficient siRNA knockdown and subsequent rescue with human patient-derived alleles. Spheroids develop apicobasal polarity and complete lumens, and they are consequently an ideal model ...
Le Floc'h Audrey A Immunology Program, Memorial Sloan-Kettering Cancer Center, Box 514, 1275 York Ave, New York, NY, 10065, USA, - - 2014
Transient,specialized cell-cell interactions play a central role in leukocyte function by enabling specific intercellular communication in the context of a highly dynamic systems level response. The dramatic structural changes required for the formation of these contacts are driven by rapid and precise cytoskeletal remodeling events. In recent years, the immunological ...
Oliveira Bruno Rodrigues BR Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande. Av. Italia Km8, Rio Grande, RS, 96203-900, - - 2014
The OCT4 transcription factor is a crucial stem cells marker and it has been related to the cancer stem cells concept. Moreover, it has also been associated to the multiple drug resistance (MDR) phenotype. Our first results pointed out a straight relation between OCT4 and ABC transporters in K562-derivative MDR ...
Kamal Ahmed A Medicinal Chemistry & Pharmacology, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India. - - 2014
A series of imidazo[1,5-a]pyridine-benzimidazole hybrids () were prepared and evaluated for their cytotoxic activity against a panel of sixty human tumor cell lines. Among them compounds and showed significant cytotoxic activity with GI50 values ranging from 1.06 to 14.9 μM and 0.43 to 7.73 μM, respectively. Flow cytometric analysis revealed ...
Mercier Romain R Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United - - 2014
The peptidoglycan cell wall is a defining structural feature of the bacterial-kingdom. Curiously, some bacteria have the ability to switch to a wall-free or 'L-form' state. Although known for decades, the general properties of L-forms are poorly understood, largely due to the lack of their systematic analysis in the molecular ...
Morhayim Jess J Department of Internal Medicine and Erasmus MC Stem Cell and Regenerative Medicine - - 2014
Beyond forming bone, osteoblasts play pivotal roles in various biologic processes, including hematopoiesis and bone metastasis. Extracellular vesicles (EVs) have been implicated in intercellular communication via transfer of proteins and nucleic acids between cells. We focused on the proteomic characterization of nonmineralizing (NMOBs) and mineralizing (MOBs) human osteoblast (SV-HFOs) EVs ...
Kong Janay Elise JE Department of Bioengineering, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, California 90095, USA. - - 2014
Lab on a chip systems have often focused on diagnostic, chemical, and cell analysis applications, however, more recently the scale and/or precision of micro-engineered systems has been applied in developing new therapies. In this issue we highlight recent work using microfluidic and micro-engineered systems in therapeutic applications. We discuss two ...
Ramirez Yulian P YP Biochemistry and Molecular Pharmacology, University of Massachusetts Medical - - 2014
The cellular responses to two new temozolomide (TMZ) analogues, DP68 and DP86, acting against glioblastoma multiforme (GBM) cell lines and primary culture models are reported. Dose-response analysis of cultured GBM cells revealed that DP68 is more potent than DP86 and TMZ and that DP68 was effective even in cell lines ...
Pan Ming - - 2014
This paper describes the design and synthesis of amphiphilic silica nanoparticles for the stabilization of aqueous drops in fluorinated oils for applications in droplet microfluidics. The success of droplet microfluidics has thus far relied on one type of surfactants for the stabilization of drops. Surfactants are known to have two ...
Atkuri Kondala R KR Pfizer - - 2014
Advanced single cell analysis technologies (e.g. Mass Cytometry) that help in multiplexing cellular measurements in limited volumes of primary cells are critical in bridging the discovery efforts to successful drug approval. Mass cytometry is the state-of-the-art technology in multi-parametric single-cell analysis. Mass cytometers (also known as CyTOF or Cytometry by ...
Zechel Sabrina - - 2014
BackgroundCortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventricular zone of the MGE, but whether the underlying subventricular and mantle regions of the MGE are spatially patterned has ...
Kondo Yuki Y Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan - - 2014
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular developmen in detail. Here, we establish a novel in vitro experimental system, in which vascular development is induced in ...
Wang Yurong Y Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China, - - 2014
Concerns about the risk of titanium dioxide nanoparticles (TiO2 NPs) to human health and environment are gradually increasing due to their wide range of applications. In this study, cytotoxicity, DNA damage, and apoptosis induced by TiO2 NPs (5 nm) in A549 cells were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed the ...
Vanhoutteghem Amandine A Laboratoire de physiologie cérébrale, Centre National de la Recherche Scientifique, Université Paris Descartes, UMR 8118, Paris, - - 2014
Absence of mitosis and meiosis are distinguishing properties of male germ cells during late fetal and early neonatal periods. Repressors of male germ cell meiosis have been identified, but mitotic repressors are largely unknown, and no protein repressing both meiosis and mitosis is known. We demonstrate here that the zinc-finger ...
Norouzi Mohammad M Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, - - 2014
The novel strategies of skin regenerative treatment are aimed at the development of biologically responsive scaffolds capable of delivering multiple bioactive agents and cells to the target tissues. In this study, nanofibers of poly (lactic-co-glycolic acid) (PLGA) and gelatin were electrospun and the effect of parameters viz polymer concentration, acid ...
Ge Xiaojun X Department of Medical Oncology and Laboratory of Signal Transduction and Molecular Targeted Therapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
The aim of the present study was to establish, characterize and elucidate the potential mechanisms of acquired gefitinb resistance, using the A549 human lung cancer cell line. A gefitinib‑resistant A549 sub‑clone was established by exposure to escalating gefitinib concentrations over a period of 16‑24 months. Half maximal inhibitory concentration (IC50) values were ...
Sun Chen - - 2014
Quantum dots (QDs) have found a wide range of biological applications as fluorophores due to their extraordinary brightness and high photostability that are far superior to conventional organic dyes. These traits are particularly appealing for studying cell biology under cellular autofluorescence background and with long observation period. However, it remains ...
Grecco Simone Dos S SD Centro de Ciências Naturais e Humanas, Universidade Federal do ABC/SP , Brazil - - 2014
Abstract Context: Nectandra (Lauraceae) species have been used in folk medicine as an antidiarrheal, analgesic, antifungal, etc., and have many pharmacological proprieties. Objective: Investigation of the chemical composition and cytotoxicity of essential oil from Nectandra leucantha Nees & Mart. leaves. This is the first study involving N. leucantha reported in ...
Tabbaa Suzanne S Department of Bioengineering and Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, - - 2014
The major limitation of large tissue-engineered constructs used for bone regeneration is the lack of vasculature and, therefore, lack of transport of essential nutrients, chemical factors and progenitor cells. Research approaches to improve the transport properties of large scaffolds focus on using angiogenic factors and vasculogenic cells to create new ...
Khattar Vinayak V Southern Research Institute, Birmingham, AL, - - 2014
Constitutive levels of Cks1 protein are very high in mammary carcinoma tissue and in breast tumor cell lines. However, despite being transcribed at relatively high levels, Cks1 protein is very low in normal mammary tissue. Also, basal Cks1 is barely detectable in primary human mammary epithelial cells (HMECs). Epoximicin, a ...
Bongard Dirk D Institute of Chemistry of New Materials, University of Osnabrück, Barbarastr. 7, D-49076 Osnabrück, Germany. - - 2014
The synthesis of two types of viologen dendrimers with peripheral carboxyl groups is described. Their interaction with plasmid DNA and CT-DNA and the influence of time evolution and electrolyte on dendriplex formation have been electrochemically investigated. A negative potential shift appearing in the cyclic voltammograms of the dendrimers indicates dendriplex ...
Xu Yanping Y Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA, - - 2014
A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the ...
Pegeot Mathieu M Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38027 Grenoble, France CNRS, IBS, F-38027 Grenoble, France CEA, DSV, IBS, F-38027 Grenoble, - - 2014
Through its ability to interact with proteins, heparan sulfate (HS) fulfills a large variety of functions. Protein binding depends on the level of HS sulfation and epimerization which are cell specific and dynamically regulated. Characterization of this molecule, however, has been restricted to oligosaccharide fragments available in large amount for ...
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