Search Results
Results 51 - 100 of 1717
1 2 3 4 5 6 7 8 9 10 >
Vazquez Hector M HM *Department of Biology, University of Fribourg, - - 2014
Temperature sensitive cdc1(ts) mutants are reported to stop the cell cycle upon shift to 30°C in early G2, i.e. as small budded cells having completed DNA replication but unable to duplicate the spindle pole body. A recent report showed that PGAP5, a human homologue of CDC1, acts as a phosphodiesterase ...
Kourakis Matthew J MJ Department of Molecular, Cell and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, 93106, - - 2014
Despite its importance in development and physiology the planar cell polarity (PCP) pathway remains one of the most enigmatic signaling mechanisms. The notochord of the ascidian Ciona provides a unique model for investigating the PCP pathway. Interestingly, the notochord appears to be the only embryonic structure in Ciona activating the ...
Park Eun-Jung EJ Department of Molecular Science and Technology, Ajou University, Suwon, 443-749, Korea, - - 2014
Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ...
Mikeš J J Institute of Biology and Ecology, Faculty of Sciences, P. J. Šafárik University in Košice, Košice, Slovakia. - - 2014
Labelling of DNA in replicating cells using 5-bromo-2´-deoxyuridine (BrdU) is widely used, however the rapid clearance and metabolisation of BrdU in the living organism is a critical issue. Although the pharmacokinetic of BrdU in experimental animals is empirically approximated, the exact time-curve remains unknown. Here we present novel method for ...
Jung In-Soo IS College of Life Sciences and Biotechnology, Korea University, Seoul, - - 2014
Extremely low-frequency magnetic fields (ELF-MFs) affect various cellular processes and systems, such as cell proliferation, differentiation and metabolic pathways. The present study investigated ELF-MFs effect on nerve growth factor (NGF) induced neuronal differentiation of PC12 cells using proteomic applications to understand its role in the enhancement of neuronal differentiation. After ...
Liang Quanfeng Q State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, - - 2014
Engineering microorganisms capable of accumulating multiple products are sometimes attractive because they yield several advantages in balancing the in vivo metabolic flux and restoring the optimal cell physiology. With the development of metabolic engineering and synthetic biology, numerous strategies for minimizing the substrate waste, optimizing the product portfolios, and maximizing ...
Teh J T JT Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, - - 2014
Isoprenylcysteine carboxylmethyltransferase (Icmt) catalyzes the last of the three-step posttranslational protein prenylation process for the so-called CaaX proteins, which includes many signaling proteins, such as most small GTPases. Despite extensive studies on Icmt and its regulation of cell functions, the mechanisms of much of the impact of Icmt on cellular ...
Piotrowska U U Department of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Warsaw, - - 2014
The endocrine activity of the thyroid gland is accomplished by its follicular and parafollicular cells. In these cells, numerous G proteins-dependent pathways are active and potentially could be regulated by a 33-kDa cytoplasmic protein phosducin, which interacts with the Gβ subunit and may compete with Gα or Gβγ dimer effectors. ...
Podlipec Rok - - 2014
Understanding biocompatibility of materials and scaffolds is one of the main challenges in the field of tissue engineering and regeneration. Complex nature of cell-biomaterial interaction requires extensive preclinical functionality testing by studying specific cell responses to different biomaterial properties, from morphology and mechanics to surface characteristics at the molecular level. ...
Kuang Yi Y Brandeis University, United - - 2014
Emerging evidence reveals that prion-like structures play important roles to maintain the well-being of cells. While self-assembly of small molecules also afford prion-like nanofibrils (PriSM), little is known on the functions and mechanisms of PriSM. Previous works demonstrated that PriSM formed by a dipeptide derivative selectively inhibit the growth of ...
Balish Mitchell F MF Department of Microbiology, Miami University, Oxford, OH 45056, USA - - 2014
In recent decades, bacterial cell biology has seen great advances, and numerous model systems have been developed to study a wide variety of cellular processes, including cell division, motility, assembly of macromolecular structures, and biogenesis of cell polarity. Considerable attention has been given to these model organisms, which include Escherichia ...
Marie Dominique D Sorbonne Universités UPMC (PARIS 06), UMR 7144 and FR2424, Station Biologique de Roscoff, Place Georges Teissier, 29688, Roscoff, France; CNRS, UMR 7144 and FR2424, Station Biologique de Roscoff, Place Georges Teissier, 29688, Roscoff, - - 2014
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of ...
Ding Xiaoyun X Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA - - 2014
Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and efficiency of currently available ...
Luo Baiwen B School of Materials Science and Engineering, Nanyang Technological University, Singapore, - - 2014
Natural materials are promising alternatives to synthetic materials used in tissue engineering applications as they have superior biocompatibility and promote better cell attachment and proliferation. Ovalbumin, a natural polymer found in avian egg white, is an example of a nature-derived material. Despite the availability and reported biocompatibility of ovalbumin, limited ...
Chung Jinyang J Center for Environment, Health and Welfare Research, Department of Energy and Environmental Engineering, Korea University of Science and Technology (UST), Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of - - 2014
Fast and accurate pathogen detection in aquatic environments is challenging in many biomedical studies and microbial diagnostic applications. In this study, we developed a real-time, continuous, and non-destructive single cell detection method using target specific aptamer-conjugated fluorescent nanoparticles (A-FNPs) and an optofluidic particle-sensor platform. A-FNPs selectively bound to the surfaces ...
Dunlop E A EA Institute of Cancer and Genetics, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: - - 2014
Mechanistic target of rapamycin (mTOR) functions as a key homeostatic regulator of cell growth and orchestrates whether anabolic or catabolic reactions are favoured. mTOR complex 1 (mTORC1) manages multiple biosynthetic pathways and promotes cell growth when nutrients are in plentiful supply. Many advances have been made over the last decade ...
Maza Itay I The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, - - 2014
Xenopus oocytes can epigenetically reprogram mouse somatic cells toward totipotency. In this issue, Jullien et al. (2014) now describe rapid, interdependent molecular events that facilitate this reprogramming.
Zou Shu-Ping SP Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of - - 2014
The enantioselective resolution of (R,S)-2,3-dichloro-1-propanol ((R,S)-DCP) to (S)-DCP by whole cells of a recombinant Escherichia coli expressing halohydrin dehalogenase (HHDH) activity was limited by product inhibition. To solve this problem to improve the productivity of (S)-DCP, an n-heptane-aqueous biphasic system was adopted in this work. The influential operational parameters including ...
Shinagawa Masaaki M Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan; Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, - - 2014
The precise identities of the virulence factors of Pseudomonas aeruginosa after bacteriolysis are still unknown. In the present study, we identified PA0423 protein, which was isolated from the Pseudomonas PAO-1 strain, as the factor responsible for cytotoxicity in lung epithelial cells. Whole bacterial cell lysate of P. aeruginosa PAO-1 caused ...
Wu Pengxiang - - 2014
Water permeability of the plasma membrane plays an important role in making optimal cryopreservation protocols for different types of cells. To quantify water permeability effectively, automated cell volume segmentation during freezing is necessary. Unfortunately, there exists so far no efficient and accurate segmentation method to handle this kind of image ...
Stahl John S JS Louis Stokes Cleveland Dept. of Veterans Affairs Medical Center - - 2014
Mutation of the Cacna1a gene for the P/Q (CaV2.1) calcium channel invariably leads to cerebellar dysfunction. The dysfunction has been attributed to disrupted rhythmicity of cerebellar Purkinje cells, but the hypothesis remains unproven. If irregular firing rates cause cerebellar dysfunction, then the irregularity and behavioral deficits should co-vary in a ...
Nowotarski Stephanie H SH *Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC - - 2014
Actin-based protrusions are important for signaling and migration during development and homeostasis. Defining how different tissues in vivo craft diverse protrusive behaviors using the same genomic toolkit of actin regulators is a current challenge. The actin elongation factors Diaphanous and Enabled both promote barbed-end actin polymerization, and can stimulate filopodia ...
Macaulay Angus D - - 2014
Even after several decades of quiescent storage in the ovary, the female germ cell is capable of reinitiating transcription to build its reserves that are essential to support early embryonic development. In the current model of mammalian oogenesis, there exists bilateral communication between the gamete and the surrounding cells that ...
Cambré Alexander A Laboratory of Gene Technology, Department Biosystems, KU Leuven, B-3001 Heverlee, Belgium; Laboratory of Food Microbiology, Department of Microbial and Molecular Systems, KU Leuven, B-3001 Heverlee, - - 2014
Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the ...
Elliott Thomas S TS Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United - - 2014
The site specific, co-translational introduction of unnatural amino acids into proteins produced in cells has been facilitated by the development of the pyrrolysyl-tRNA synthetase/tRNACUA pair. This pair can now be used to direct the site-specific incorporation of designer amino acids in E. coli, yeast, mammalian cells, and animals (the worm, ...
Oikawa Shino S Department of Physiology, Nippon Medical School Graduate School of Medicine, Tokyo, - - 2014
Background/Aims: We previously suggested that a non-neuronal cholinergic system modulates energy metabolism through the mitochondria. However, the mechanisms responsible for making this system crucial remained undetermined. Methods: In this study, we developed a fusion protein expression vector containing a luciferase gene fused to the folic acid receptor-α gene. Results: This ...
Fu Yun Y Department of Molecular Biology and Biochemistry, Xinxiang Medical University, Xinxiang, Henan, P.R. - - 2014
The Mannich base containing ciprofloxacin and kojic acid structural units was prepared and evaluated in antitumor activity. The enhancement in antitumor activity was observed both from the Mannich base (IC50: 103.3±5.0 µM for HepG2, 87.9±8.0 µM for HCT-116 cell) and its copper complex (IC50: 11.5±1.8 µM for HepG2, 44.4±2.5 µM for HCT-116 cell) compared ...
Ungvarsky Jan J Department of Organic Chemistry, Moyzesova 11, 04001 Kosice, Slovak - - 2014
A novel series of trisubstituted acridines were synthesized with the aim of mimicking the effects of BRACO19. These compounds were synthesized by modifying the molecular structure of BRACO19 at positions 3 and 6 with heteroacyclic moieties. All of the derivatives presented in the study exhibited stabilizing effects on the human ...
Nakkala Jayachandra Reddy JR Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605 014, - - 2014
Nanomedicine utilize biocompatible nanomaterials for diagnostic and therapeutic purposes. This study reports the synthesis of silver nanoparticles using aqueous rhizome extract of Acorus calamus (ACRE) and evaluation of antioxidant, antibacterial as well as anticancer effects of synthesized A. calamus silver nanoparticles (ACAgNPs). The formation of ACAgNPs was confirmed by UV-visible ...
García Esther E Department of Cellular and Molecular Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus UAM Cantoblanco, Darwin 3, 28049 Madrid, Spain. Electronic address: - - 2014
Actin filament assembly and reorganisation during cell migration and invasion into extracellular matrices is a well-documented phenomenon. Among actin-binding proteins regulating its polymerisation, the members of the WASP (Wiskott Aldrich Syndrome Protein) family are generally thought to play the most significant role in supporting cell invasiveness. In situ, cytosolic N-WASP ...
Re Angela A Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Via delle Regole 101, I38123 Trento, Italy; Center for Biological Sequence Analysis, Technical University of Denmark, Kemitorvet, DK2800 Lyngby, - - 2014
The shortage of molecular information on cell cycle changes along embryonic stem cell (ESC) differentiation prompts an in silico approach, which may provide a novel way to identify candidate genes or mechanisms acting in coordinating the two programs. We analyzed germ layer specific gene expression changes during the cell cycle ...
Kota Swathi S Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400 084, India. - - 2014
PprA, a pleiotropic protein involved in radioresistance of Deinococcus radiodurans was detected in multiprotein DNA processing complex identified from this bacterium. pprA mutant expressing GFP-PprA could restore its wild type resistance of γ radiation. Under normal conditions, GFP-PprA expressing cells showed PprA localization on both septum trapped nucleoids (STN) and ...
Khanna Rajnish R Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA 94305, - - 2014
Reorganization of the cortical microtubule cytoskeleton is critical for guard cell function. Here, we investigate how environmental and hormonal signals cause these rearrangements and find that COP1, a RING-finger-type ubiquitin E3 ligase, is required for degradation of tubulin, likely by the 26S proteasome. This degradation is required for stomatal closing. ...
Vickers Dwayne A L DA The Rowland Institute, Harvard - - 2014
Electroporation has received increasing attention in the past years, because it is a very powerful technique for physically introducing non-permeant exogenous molecular probes into cells. This work reports a microfluidic electroporation platform capable of performing multiple molecule delivery to mammalian cells with precise and molecular-dependent parameter control. The system's ability ...
Marinova Zoya Z University Clinics for Child and Adolescent Psychiatry, University of Zürich; - - 2014
Many brain-related disorders have neuronal cell death involved in their pathophysiology. Improved in vitro models to study neuroprotective or neurotoxic effects of drugs and downstream pathways involved would help gain insight into the molecular mechanisms of neuroprotection/neurotoxicity and could potentially facilitate drug development. However, many existing in vitro toxicity assays ...
O'Brien Jason M JM Environmental Health Science and Research Bureau, Health Canada, Environmental Health - - 2014
De novo mutations arise mostly in the male germline and may contribute to adverse health outcomes in subsequent generations. Traditional methods for assessing the induction of germ cell mutations require the use of large numbers of animals, making them impractical. As such, germ cell mutagenicity is rarely assessed during chemical ...
Newell Evan W EW Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), 8A Biomedical Grove, Immunos #03-06, Singapore, 138648, Singapore, - - 2014
Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, ...
Borlinghaus Jan J Department of Plant Physiology, RWTH Aachen University, 52056 Aachen, - - 2014
Allicin (diallylthiosulfinate) is a defence molecule from garlic (Allium sativum L.) with a broad range of biological activities. Allicin is produced upon tissue damage from the non-proteinogenic amino acid alliin (S-allylcysteine sulfoxide) in a reaction that is catalyzed by the enzyme alliinase. Current understanding of the allicin biosynthetic pathway will ...
Koirala Santosh S Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, - - 2014
Many bacteria are motile only when nutrients are scarce. In contrast, Salmonella enterica serovar Typhimurium is motile only when nutrients are plentiful, suggesting that this bacterium uses motility for purposes other than foraging, most likely for host colonization. In this study, we investigated how nutrients affect motility in S. enterica and ...
Chaves-Olarte Esteban E Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, 11501-2060, Costa Rica, - - 2014
The pathogenesis of brucellosis depends on the ability of bacteria from the genus Brucella to invade and replicate within animal cells. To understand the molecular pathways used by Brucella spp. to reach its intracellular niche, robust and reproducible bacteria purification protocols that provide enough material for biochemical and molecular biology ...
Cerveira Joana F JF Unidade de Química-Física Molecular and Departamento de Ciências da Vida, Universidade de Coimbra, 3000-456 Coimbra, Portugal ; Centro de Biología Molecular "Severo Ochoa", CIBERER, Instituto de Investigación Hospital 12 de Octubre, Universidad Autónoma de Madrid, 28049 Madrid, - - 2014
Previous studies on the impact of hexavalent chromium [Cr(VI)] on mammalian cell energetics revealed alterations suggestive of a shift to a more fermentative metabolism. Aiming at a more defined understanding of the metabolic effects of Cr(VI) and of their molecular basis, we assessed the impact of a mild Cr(VI) exposure ...
Pan Xinghua X Department of Genetics, School of Medicine, Yale University, New Haven, CT, - - 2014
Single-cell analysis heralds a new era that allows "omics" analysis, notably genomics, transcriptomics, epigenomics and proteomics at the single-cell level. It enables the identification of the minor subpopulations that may play a critical role in a biological process of a population of cells, which conventionally are regarded as homogeneous. It ...
Song Congdi C Department of Biology II, Center for Integrated Protein Science Munich (CIPSM), Ludwig Maximilians University Munich, Grosshadernerstrasse 2, 82152 Planegg-Martinsried, - - 2014
Methyl-CpG binding protein 2 (MECP2) is a protein that specifically binds methylated DNA, thus regulating transcription and chromatin organization. Mutations in the gene have been identified as the principal cause of Rett syndrome, a severe neurological disorder. Although the role of MECP2 has been extensively studied in nervous tissues, still ...
Saxe Friederike F Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam-Golm, 14476, - - 2014
X-ray scattering is a well-established method for measuring cellulose microfibril angles in secondary cell walls. However, little data is available on the much thinner primary cell walls. Here, we show that microfibril orientation distributions can be determined by small angle X-ray scattering (SAXS) even in primary cell walls. The technique ...
Lee Jaehee J Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania - - 2014
Introduction of exogenous genetic material, such as DNA, mRNA, siRNA, or miRNA, into cells is routinely performed using one of the many different standardized methods, including lipid-mediated transfection, electroporation, and microinjection to identify their biological function. The ability to control the location and amount of nucleic acids introduced into a ...
Lin Yao-Cheng YC 1] Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent B-9052, Belgium [2] Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent B-9052, Belgium - - 2014
The HEK293 human cell lineage is widely used in cell biology and biotechnology. Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation ...
Quester S S Department of Chemistry, Institute of Physical Chemistry, University of Cologne, 50939 Cologne, - - 2014
We have generated closed-cell microcellular foams from gliadin, an abundantly available wheat storage protein. The extraction procedure of gliadin from wheat gluten, which involves only the natural solvents water and ethanol, respectively, is described with emphasis on the precipitation step of gliadin which results in a fine dispersion of mostly ...
Gedye Craig A CA Princess Margaret Cancer Centre, University Health Network, Toronto, ON, - - 2014
Cell surface proteins have a wide range of biological functions, and are often used as lineage-specific markers. Antibodies that recognize cell surface antigens are widely used as research tools, diagnostic markers, and even therapeutic agents. The ability to obtain broad cell surface protein profiles would thus be of great value ...
Lee Ling Yen LY Department of Chemistry and Biomolecular Sciences, Biomolecular Frontiers Research Centre, Macquarie University , Sydney, NSW , - - 2014
Glycoproteins perform extra- and intracellular functions in innate and adaptive immunity by lectin-based interactions to exposed glyco-determinants. Herein, we document and mechanistically explain the formation of subcellular-specific N-glycosylation determinants on glycoproteins trafficking through the shared biosynthetic machinery of human cells. LC-MS/MS-based quantitative glycomics showed that the secreted glycoproteins of eight ...
Lu Jun J Chemical Sciences and Engineering Division, Argonne National Laboratory, Lemont, Illinois 60439, - - 2014
Lithium-oxygen batteries have the potential needed for long-range electric vehicles, but the charge and discharge chemistries are complex and not well understood. The active sites on cathode surfaces and their role in electrochemical reactions in aprotic lithium-oxygen cells are difficult to ascertain because the exact nature of the sites is ...
1 2 3 4 5 6 7 8 9 10 >