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Thompson A B - - 1996
The method of preparation of bronchoalveolar lavage fluid (BALF) for cytological examination can significantly affect the results of cellular quantitation. Investigations have shown that cytocentrifugation leads to an underestimation of the number of lymphocytes and membrane filter preparation to an underestimation of the number of neutrophils. As a simple alternative ...
Visser M J - - 1996
For in vitro evaluation of functional properties of endothelial cells seeded on synthetic vascular prostheses accurate and reproducible quantification of cells is mandatory. Comparison of these properties with those resulting from other studies requires correlation of the functional parameters to reliably counted cell numbers. The accuracy of methods of quantification ...
Edelman M J - - 1996
OBJECTIVES: Intraoperative autotransfusion of shed blood is widely utilized in surgery. However, several studies have raised concern about the transmission of tumor cells during oncologic procedures. We compared the ability of a leukocyte depletion filter (RC-400; LDF) to a standard red blood cell filter (SBF) to remove tumor cells derived ...
Wong H H - - 1996
The settling characteristics of cell debris and inclusion bodies prior to, and following, fractionation in a disc-stack centrifuge were measured using Cumulative Sedimentation Analysis (CSA) and Centrifugal Disc photoSedimentation (CDS). The impact of centrifuge feedrate and repeated homogenisation on both cell debris and inclusion body collection efficiency was investigated. Increasing ...
Isaacson L - - 1996
1. Using cyclic AMP to stimulate perfused tsetse fly Malpighian tubules bathed in SO42- Ringer frequently causes an immediate but transient peak in transtubular potential (Vt), before stabilisation of Vt at an increased value. 2. These transients were investigated by monitoring the associated changes in cable properties and current&shy;voltage (I/V) ...
Bischoff F Z - - 1995
Recovering and analysing fetal erythrocytes from maternal blood is being pursued for non-invasive prenatal genetic diagnosis. We report the observation of 46,XY/47,XXY mosaicism in fetal cells from a woman whose first-trimester chorionic villus sampling (CVS) initially showed only 46,XY. Only after exhaustive (500 cells) analysis were four XXY cells found ...
Joyner R E - - 1995
Erythrocytes resistant to standard lysing reagents are known to occur in sickle cell disease. These lyse-resistant erythrocytes can cause aberrant automated leucocyte counts and differentials. The ability of the Cell-Dyn 3500 automated haematology analyser to eliminate resistant erythrocytes and accurately count and differentiate leucocytes was evaluated. Samples were obtained from ...
Tockman M S - - 1995
OBJECTIVE: To compare the safety and efficacy of homogenizing sputum with deoxyribonuclease I (DNAse I), dithiothreitol (DTT), N-acetyl-L-cysteine, sodium EDTA and trypsin against standard mechanical blending to provide mucus-free, single-cell suspensions for quantitative analysis. STUDY DESIGN: Clinical sputum specimens or cultured human bronchogenic carcinoma cells were preserved in 2% polyethylene ...
Tutschek B - - 1995
Transcervical cell (TCC) samples have been shown to contain fetal cells amenable to molecular analysis. However, the presence of 'contaminating' maternal cells limits their use for prenatal diagnoses. In this report we show that clumps of fetal cells can be isolated from transcervical samples by micromanipulation and tested by fluorescence ...
Meshulam T - - 1995
Studies of antimycotic host defenses have been limited by the paucity of rapid, reproducible quantitative assays for fungal cell damage. Prior studies defined a colorimetric method that uses MTT, a tetrazolium dye, to quantify polymorphonuclear leukocyte (PMNL)-mediated damage to fungi. These relatively simple, rapid, and reproducible assays require cumbersome extraction ...
Beliën J A - - 1995
The proportion of exfoliated buccal mucosal cells with micronuclei gives the opportunity to assess sensitivity to gamma-radiation and genotoxic compounds and in addition to monitor the effectiveness of cancer intervention strategies. So far, results on counting micronuclei in various publications are difficult to compare because of differences in methods used, ...
Lewinson D - - 1995
Tartrate-resistant acid phosphatase (TRAP) histochemistry has been used to follow chondro/osteoclasts and their precursors during ontogenesis of the mandibular condyle of the mouse (from day 16 of gestation until day 15 of neonatal life). TRAP+ mono-, bi-, and multinuclear cells were counted separately in the perichondrium, along the resorption front ...
Hinrichs R - - 1995
To combine the advantages of solid scintillation and the use of 96-well plates, an in-plate cell proliferation assay was designed using scintillation wax. Messy, conventional liquid wastes are not produced, thereby making solid scintillation safer and more convenient. Additionally, the use of optimized, 96-well ViewPlates for inplate counting procedures allows ...
Li Q - - 1995
Silver staining techniques developed to demonstrate argyrophilic nucleolar organizer regions (Ag-NORs) have been widely applied in a variety of cell kinetic studies, using the mean number of AgNORs in tumour cells as a marker for malignancy of certain types of neoplasms. However, the AgNOR techniques currently available are not entirely ...
Pascual E - - 1995
The objective of this study was to determine whether monosodium urate (MSU) crystals are phagocytosed in the synovial fluid (SF) of the asymptomatic joints of patients with gout. SF samples were obtained from 20 asymptomatic knees of 19 different patients. Cell and differential counts were done. Intracellular MSU crystals were ...
Clarke T - - 1995
The requirement for accuracy in the counting of somatic cells in milk has been increased by the use of this measurement as a basis of payment for milk. This paper reports on several factors that can substantially affect efficiency of staining and cell detection in various types of fluorescent cell ...
Carl Van Geem
The goal of this paper is to estimate in a theoretical way the reduction of the number of cells of, and the overall number of neighbouring cells in, a grid when some cells in a uniform grid are replaced by a larger cell in an overlaying non-uniform grid. We assume ...
Suhr H - - 1995
A new technique is presented which allows the use of a front-end sensor head for in situ and on-line characterization of cell concentration and cell size during fermentation. An epifluorescence microscope is mounted in a port of a bioreactor viewing directly into the agitated broth. Still images from cells are ...
Oliver J D - - 1995
Using plate counts, total cell counts, and direct viable counts, we examined the fate of cells of Vibrio vulnificus placed into natural estuarine waters during both winter and summer months. Cells inoculated into membrane diffusion chambers and placed into estuarine waters entered into a viable but nonculturable (VBNC) state in ...
Coventry B J - - 1995
High-sensitivity immunoperoxidase labelling can be achieved using heavy metal enhancement of the di-amino (DAB) reaction product. For example nickel chloride combined with DAB improves the sensitivity of the method approximately 7-10 fold. This allows detection of approximately 100-200 molecules on cell surfaces. This has an obvious advantage over standard non-enhanced ...
Zheng Y L - - 1995
We investigated a case of massive feto-maternal bleeding by using negative magnetic cell sorting (MACS) and fluorescent in situ hybridization (FISH). A 37-year-old pregnant woman had an uncomplicated amniocentesis for advanced maternal age at 16 weeks' gestation. The fetal karyotype was 46,XY. At 19 weeks' gestation, she had a minor ...
Dziadkowiec D - - 1995
Skimmed milk powders were spiked with one of three Salmonella serovars and incubated in buffered peptone water for 24 h. No false-negative results were obtained by immunomagnetic separation (IMS), compared to seven for selenite cysteine, one for Müller-Kauffmann tetrathionate and two for Rappaport-Vassiliadis enrichment broths. Salmonella virchow was detected and ...
Hansson M - - 1995
Mobilization of primitive haematopoietic cells to the peripheral blood was studied in 25 patients with haematological malignancies. The optimal level of peripheral stem cells (PSC), defined by their surface expression of CD34, was significantly higher after mobilization with G-CSF, either following chemotherapy or alone (median: 123 x 10(6)/l and 143 ...
Takahashi M - - 1995
Cell proliferation in the endolymphatic sac (ES) after immunostimulation was assessed immunohistochemically. Guinea pigs were employed as experimental animals and keyhole limpet hemocyanin was used as the antigen. After i.p. injection of 5-bromo-2'-deoxyuridine (BrdUrd), the BrdUrd+ cells were counted to assess the number of proliferating cells. In the secondary immune ...
Malmierca M S - - 1995
In this paper a modern statistical method is applied to an old cell classification and identification problem in the central nucleus of the inferior colliculus. In a recent computer-based reconstruction study of Golgi-impregnated neurons in the rat, two types of cell with flattened dendritic arbors, flat (F) and less flat ...
Sahr F - - 1995
The lack of standard local values for use as reference values has been a problem facing our haematological units in Sierra Leone for a long time. As a preliminary step towards the establishment of standard local values, one hundred and sixty apparently healthy Sierra Leoneans between the ages of 10 ...
Rogers G W - - 1995
Genetic and phenotypic correlations were estimated for means of log2 SCC (somatic cell scores) from the first 305 d, the first 75 d, the first 150 d, 76 to 305 d, and 151 to 305 d of lactation for first and second lactations using REML. Somatic cell scores from the ...
Del Gratta C - - 1995
We report on experiments aimed at the assessment of a new method for cell marking. This method combines superparamagnetic particles, commonly used for cell separation, linked to monoclonal antibodies, and biomagnetic instrumentation featuring an extremely high magnetic field sensitivity. The final goal of the method is to locate and estimate ...
Jiang C - - 1995
Two main sources of error in the conventional stained film cell count method, i.e. errors from imperfect spreading of the suspension and uneven distribution of cells in the film, have been eliminated by counting all the cells in the film. Staining solution and sodium alginate were added to the suspension ...
Popov T A - - 1995
Inhalation of hypertonic saline aerosol is a relatively noninvasive method to obtain sputum for examination of inflammatory processes in the airways. We investigated some technical factors which might influence the success of induction and sputum cell counts. In total, twenty six asthmatic and 13 healthy subjects, unable to raise sputum ...
Ramsden J J - - 1995
A novel, on-line method of accurately parametrizing the shape of spreading cells is described. The substrate on which the cells are deposited serves as an optical waveguide. The method is based on the interaction between the evanescent part of the guided waves and the cells. No special labelling of the ...
Kennelly J C - - 1995
The most labour-intensive feature of the in vivo rat liver UDS assay is the scoring of hepatocyte autoradiograms by microscope. Even with image analyser and computer equipment the scoring phase of a full study might require half of the technical effort applied. Practice recommended by guidelines has been to score ...
Becker F F - - 1995
Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic ...
Lelièvre, J.
Assessed by the micromanipulation technique, the main mechanical properties of blood cells (red cell and white cell) are reviewed. Hyperelasticity of red blood cell membrane is characterized and corresponding material coefficients are given. Surface tension and cell viscosity of leukocyte are discussed and related values are stated. Some aspects of ...
Sakagami K - - 1995
A new, simple method for isolating retinal pigment epithelial (RPE) cells from rat eyeballs was developed: incubation of the eyeballs in 0.1% proteinase K solution. This method yielded an average of 4 x 10(4) viable RPE cells from each Sprague-Dawley rat eyeball in 1 h. These RPE cells were hexagonal ...
Bedö S - - 1995
Variations in the yield, composition and somatic cell count of milk were studied in 30 grazing ewes, representing the Pleven F1 x East-Friesian Blackface, Pleven F1 and Merino genotypes, during five months of lactation. The somatic cell count (SCC) correlated negatively with milk yield and lactose whilst positively with milk ...
Park C S - - 1995
OBJECTIVES: To elucidate the presence of neutrophil chemotactic factor (NCF) and characterize them in the bronchial trees of symptomatic patients with bronchial asthma. METHODS: Bronchoalveolar lavage (BAL) fluids were concentrated by ultrafiltration. Differential counts of BAL cells was performed upto 500 cells on the cytocentrifuge-prepared slides. NCF activities in concentrated ...
KÜLtz D - - 1995
Long-jawed mudsuckers (Gillichthys mirabilis) were acclimated to sea water (SW) at 7 &deg;C, SW at 26 &deg;C or dilute sea water (DSW) at 26 &deg;C for 5 months. Gill cells were isolated and the proportion of mitochondria-rich (MR) cells was determined. The number of cells harvested amounted to 4.7x10(7)&plusmn;0.6x10(7) to ...
Yamaguchi, Shinya
Samples of stage I to III non-small cell pulmonary carcinoma, resected from 51 patients, were simultaneously doublestained for Ki-67 and AgNOR (argyrophil nucleolar organizer region). The responses to the staining was analyzed in relation to the stage, degree of histological differentiation, extent of pulmonary tumor (factor T), extent of lymph ...
Büsch J - - 1994
For simple and effective isolation of fetal cells from peripheral maternal blood, we combined depletion of maternal cells and enrichment of fetal cells by high-gradient magnetic cell separation (MACS). First CD45+ and CD14+ cells were depleted from maternal peripheral blood mononuclear cells by MACS. From the depleted fraction, CD71+ erythroid ...
Abbasoglu O - - 1994
To assess the effect of peritoneal lavage on peritoneal cellular defense mechanisms, peritoneal fluid total cell counts, cell types, phagocytic activity and polymorphonuclear leukocytes (PMNL) chemotaxis before and 4 hours after saline lavage was studied on 70 rats. Lavage with 10 ml saline decreased the peritoneal total cell counts of ...
Mesker W E - - 1994
The detection of rare-event cells circulating in peripheral blood using automated image analysis was evaluated using a model system consisting of cells from a breast cancer cell line (SKBR3) seeded in a mononuclear cell suspension. Slides of cells with optimal morphology were prepared according to an optimized preparation procedure based ...
Allen Van Gelder,Jane Wilhelms
A popular technique for rendition of isosurfaces in sampled data is to consider cells with sample points as corners and approximate the isosurface in each cell by one or more polygons whose vertices are obtained by interpolation of the sample data. That is, each polygon vertex is a point on ...
Bianchi D W - - 1994
OBJECTIVE: The detection of fetal aneuploidy and gene mutations by analysis of fetal cells in maternal blood has demonstrated the feasibility of noninvasive prenatal diagnosis. Fetal cells are rare in the maternal circulation; all current methods used for their isolation also yield maternal cells. We developed a method that permits ...
Stenersen T C - - 1994
Monolayer preparation from paraffin-embedded tissue from archival biopsy specimens older than 10 years was shown in this study to be more difficult to deal with than recent biopsies. We have studied the disseminative effect of several enzymes on archival biopsies from laryngeal epithelium, as well as mechanical dissemination. Thirty minutes ...
Bovill R A - - 1994
The fluorogenic redox indicator 5-cyano-2,3-ditolyltetrazolium chloride (CTC) was compared with the chromogenic p-iodonitrotetrazolium violet (INT) and conventional methods to assess cellular viability. Mild heat treatment was used as well-controlled method for producing non-viable and sub-lethally injured cells. CTC gave an underestimation of the viability of Listeria monocytogenes cells when compared ...
Vray B - - 1994
Macrophages harvested from the murine peritoneal cavity are functionally and morphologically heterogeneous. Here, we describe a procedure which permits the determination of specific cell densities using a continuous density gradient of Percoll (analytical step). Subsequently, discontinuous density gradients are used in routine (preparative step) to isolate all the cell subpopulations ...
Peter Kornerup
A very simple multiplier cell is developed for use in a linear, purely systolic array forming a digit-serial multiplier for unsigned or 2'complement operands. Each cell produces two digit-product terms and accumulates these into a previous sum of the same weight, developing the product least significant digit first. Grouping two ...
Ferguson-Smith M A - - 1994
Current methods for the prenatal diagnosis of genetic disorders from fetal cells in the maternal circulation are restricted by maternal cell contamination and therefore must rely on the presence of features (such as Y-specific DNA sequences) absent from maternal cells. We have used density gradient centrifugation and magnetic-activated cell sorting ...
Vral A - - 1994
An improved slide preparation technique is described for the automated detection of micronuclei in binucleate cytokinesis-blocked human lymphocytes. This automated system (Discovery Image Analyser, Becton-Dickinson Image Cytometry Systems, Leiden, The Netherlands) searches for the pattern of two touching nuclei in binucleate cells instead of the edges of the cytoplasm. For ...
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