Search Results
Results 801 - 850 of 1725
< 12 13 14 15 16 17 18 19 20 21 22 >
McNiece I - - 1998
The dose of cells expressing the surface antigen CD34 (CD34+) has been shown to be a reliable predictor of the time to engraftment following transplantation of PBPC to support high-dose chemotherapy. However, evaluation of rare cells is complicated by a number of factors, including the variability in operator and technical ...
Koroleva OA - - 1998
Carbohydrate metabolism of barley (Hordeum vulgare) leaves induced to accumulate sucrose (Suc) and fructans was investigated at the single-cell level using single-cell sampling and analysis. Cooling of the root and shoot apical meristem of barley plants led to the accumulation of Suc and fructan in leaf tissue. Suc and fructan ...
Nunan KJ - - 1998
Cell walls were isolated from the mesocarp of grape (Vitis vinifera L.) berries at developmental stages from before veraison through to the final ripe berry. Fluorescence and light microscopy of intact berries revealed no measurable change in cell wall thickness as the mesocarp cells expanded in the ripening fruit. Isolated ...
Chazaud B - - 1998
We developed a short-time assay to evaluate muscle satellite cell migration, based on the fact that during myogenic differentiation, myoblasts migrate preferentially towards high cellular density areas where myotubes would form. This assay consists of a computer-assisted count of cells within a randomly chosen field, performed every hour for eight ...
Hockstein S - - 1998
OBJECTIVE: To determine which patient- and procedure-related factors contribute to maternal cell contamination in uncultured amniocentesis fluid. METHODS: One hundred thirty amniotic fluid (AF) samples were obtained by three operator groups: maternal-fetal medicine faculty (n=50), general obstetrician gynecologists (n=50), and obstetrics and gynecology residents supervised by maternal-fetal medicine faculty (n=30). ...
Doyle D L - - 1998
This study was designed to evaluate the effect of soaking in either Hank's balanced salt solution (HBSS) or milk on periodontal ligament (PDL) cell viability in avulsed teeth. Dry storage times of 30, 60, and 90 min were evaluated. PDL cell viability was determined after removal of the cells from ...
Kozobolis V P - - 1998
BACKGROUND AND OBJECTIVES: To evaluate the corneal changes after Nd:YAG laser pupillary membranectomies (group A), iridotomies (group B), and capsulotomies (group C) in a follow-up period of 6 months. PATIENTS AND METHODS: The Nd:YAG laser was applied to 3 different groups of patients. Specular microscopy, including the counting of corneal ...
Orsetti B - - 1998
Prenatal diagnosis is presently performed following invasive procedures with variable risks of fetal loss; non-invasive procedures using fetal cells in maternal blood would be welcome for the early detection of fetal sex or aneuploidy. We describe a simple and rapid protocol to detect fetal cells and thus to assess fetal ...
Krohne S G - - 1998
OBJECTIVE: To determine whether the cell measuring function of a laser flare-cell photometer is accurate and reproducible, using an in vitro model. SAMPLE POPULATION: Leukocytes from 8 clinically normal Beagles. PROCEDURE: Latex beads 11.9 and 6.4 microm in diameter were used to simulate canine WBC and RBC, respectively. Beads were ...
Robertson BR - - 1998
The forward light scatter intensity of bacteria analyzed by flow cytometry varied with their dry mass, in accordance with theory. A standard curve was formulated with Rayleigh-Gans theory to accommodate cell shape and alignment. It was calibrated with an extinction-culture isolate of the small marine organism Cycloclasticus oligotrophus, for which ...
C. R. Osterwald,S. Anevsky,A. ...
This paper presents an overview of the World Photovoltaic Scale (WPVS) international reference cell calibration program. The WPVS provides a scale for PV performance measurements that has been established through round-robin calibration of a group of primary reference cells and is traceable to Systme International (SI) units. Procedures for recalibration ...
Podgornik A - - 1998
We introduce the ratio of nonflocculent versus total biomass as a criterion for starting cell separation from the medium. This criterion can be applied for the automation of the process regardless of the process dynamics. Its minimum indicates the optimum period of time for the start of the separation process ...
Melillo G - - 1998
Various techniques are used to collect specimens from the nasal mucosa for morphologic and biochemical analysis. The purpose of this study was to devise a method that overcomes some of the disadvantages (e.g., invasive procedure, samples not suitable for cytologic and biochemical analysis, lack of standardization, and poor reproducibility) of ...
Garvin A M - - 1998
Single cell PCR is a powerful method for determining the genetic properties of individual cells. In the instance of heterozygous loci, however, preferential amplification of one allele can lead to allele drop out (ADO) of the other allele. Fortunately ADO does not occur in all amplifications, and is usually random ...
Navenot J M - - 1998
A new method to quantify red blood cells (RBCs) containing fetal hemoglobin (HbF), or F cells, by flow cytometry was developed. The use of formaldehyde as fixative agent and sodium dodecyl sulfate (SDS) to permeabilize fixed RBCs resulted in a low staining background, a negligible Hb leakage, and minimal cell ...
Martin Collier
A method is described for performing routing in three-stage ATM switches which feature multiple channels between the switch modules in adjacent stages. The method is suited to hardware implementation using parallelism to achieve a very short execution time. This allows cell-level routing to be performed, whereby routes are updated in ...
Hübl W - - 1998
Conventional flow cytometric methods for CD34+ cell counting may be affected by the high number of nucleated red blood cells or nonviable cells in cord blood and its products. We developed a simple flow cytometric no-wash procedure that avoids these shortcomings because it provides absolute CD34+ cell counts and assesses ...
Rose JK - - 1998
The Charentais variety of melon (Cucumis melo cv Reticulatus F1 Alpha) was observed to undergo very rapid ripening, with the transition from the preripe to overripe stage occurring within 24 to 48 h. During this time, the flesh first softened and then exhibited substantial disintegration, suggesting that Charentais may represent ...
Flowers A E - - 1998
The tropical marine sponge Dysidea herbacea (Keller) contains the filamentous unicellular cyanobacterium Oscillatoria spongeliae (Schulze) Hauck as an endosymbiont, plus numerous bacteria, both intracellular and extracellular. Archaeocytes and choanocytes are the major sponge cell types present. Density gradient centrifugation of glutaraldehyde-fixed cells with Percoll as the support medium has been ...
el-Harazi S M - - 1998
BACKGROUND AND OBJECTIVES: To determine the interobserver and intraobserver reproducibility of the laser flare meter and laser cell counter in assessing anterior chamber inflammation following cataract surgery. PATIENTS AND METHODS: Thirty-nine eyes with varied degrees of inflammation and 9 normal eyes were included in the study. Anterior chamber flare and ...
Holzgreve W - - 1998
OBJECTIVE: To investigate whether the transfer of fetal blood cells to the maternal circulation is perturbed in pregnancies affected by preeclampsia. METHODS: Fetal erythroblasts were isolated from eight women with clinically diagnosed preeclampsia (blood pressure values of at least 140/90 mmHg and associated proteinuria) and an equal number of matched ...
Huntenburg C C - - 1998
This review describes the published preclinical and clinical data on the use of a manual or semiautomated immunomagnetic selection device, termed the Isolex system. Preclinical evaluation of hematopoietic progenitor cells (CD34+ cells) selected from bone marrow, peripheral blood leukapheresis products, and umbilical cord blood is reviewed with respect to differentiation ...
Strasser A - - 1998
A simple method for the simultaneous separation and purification of peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil cells (PMNC) was developed for comparative and functional studies in the immune system of the dog. Purity and cell viability were > 95%, yields were similar to those obtained by other techniques ...
ten Hacken N H - - 1998
Counting of inflammatory cells in human airway biopsy specimens is difficult because immunopositive cells are present in varying density in lung tissue. The goal of our study was to assess the minimal amount of tissue that is necessary for the counting of constant cell numbers. In bronchial biopsy specimens from ...
Dubrovsky J G - - 1998
We present a field method for squash preparation of root apical meristem that does not require dry ice for cell adhesion to a slide. The method is based on the use of a weak gelatin solution as a stabilizing agent to prevent cell loss. Alcian blue 8GX was used as ...
Nógrády N - - 1998
Immunochemical methods were developed for monitoring cyclodextrin (CD) glycosyltransferase (CGTase) production and growth of an industrial CD-producing Bacillus macerans strain. Extracellular concentrations of CGTase released into a non-transparent culture medium during a 44 h long fermentation were detected by an indirect antigen inhibition enzyme-linked immunosorbent assay (ELISA). The ELISA was ...
Mancini A - - 1998
In a previous paper it was demonstrated that Coenzyme Q10, a lipidic molecule with important antioxidant properties, is present at remarkable levels in human seminal fluid, and shows a direct correlation with seminal parameters (sperm count and motility). In patients with varicocele, on the contrary, correlation with sperm motility was ...
Beckert, Rolf
The aim of this investigation is to show, whether totally different calibration methods (absolute spectral response at Fraunhofer ISE, blackbody at various temperatures at SERC) give similar results for the performance of TPV cells under infrared spectra. Two cell types (InGaAs and GaSb) have been tested, applying two different methods ...
Ben-Ezra J - - 1998
Urinalysis is a high-volume procedure that currently requires significant labor to examine microscopic sediment. We evaluated the Sysmex UF-100 automated urinalysis analyzer for performing this task. Instrument accuracy was assessed by comparing continuous counts of microscopic elements from the UF-100 with ranges of cells (per low-power field or high-power field) ...
Crespo R - - 1998
Air sac lavage has become a routine diagnostic tool used in pet birds; however, cytologic techniques for collection and handling of avian cells have not been reported. In this study, directed endoscopy was used to obtain the air sac washes. Different factors were compared that might affect final preservation of ...
de Pablo M A - - 1998
A mixture of Ficoll 400 and sodium diatrizoate (Hypaque) at a density of 1.077 g/ml has been used to isolate the mononuclear cells from the remaining haematic cells. A simple, inexpensive and classical method was established to obtain substantially erythrocyte-free polymorphonuclear cell preparations from mouse peripheral blood, using a mixture ...
Bohmer R M - - 1998
The maternal peripheral blood circulation can serve as a source of fetal cells for the prenatal diagnosis of genetic abnormalities, eliminating the need for fetal cell sampling by invasive techniques. However, the extreme scarcity of these cells leads to a variety of biological questions and technical hurdles on the way ...
Daniel V - - 1998
Flow cytometry technique is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antrbodies or ligands (e.g., cytokines, hormones) that bind specific cell-assocrated molecules. Flow cytometry is quantitative, fast (analysis of >l0,000 cells/min), reliable, and reproducible. Cells can be analyzed individually and simultaneously for several different characteristics or parameters (e.g., ...
Nelson M - - 1998
BACKGROUND AND OBJECTIVES: The Kleihauer slide test is in general use to screen obstetric patients for possible fetomaternal haemorrhage. Since 1993, Rh(D)-negative patients have been tested in our laboratory by a flow-cytometric method detecting Rh(D)-positive fetal cells, a method which offers improved sensitivity and accuracy. We report another flow-cytometric method ...
Pal U - - 1998
Automatic cell segmentation has various application potentials in cytometry and histometry. In this paper, an automatic cluster (touching) cell segmentation approach using the dominant contour feature points has been presented. Dominant feature points are the locations of indentation on the contour of the cluster. First, dominant feature points on the ...
Kelly J D - - 1997
OBJECTIVES: To derive and validate a rapid method for calculating apoptotic indices in superficial transitional cell carcinoma (TCC) as a measure of chemosensitivity to mitomycin. MATERIALS AND METHODS: Apoptotic cells, identified by light microscopy in 20 superficial TCC specimens, were expressed as an index of the total tumour cell population ...
Büsche G G Pathologisches Institut der Medizinischen Hochschule, Hannover, - - 1997
A new parametric method is presented, called "square sampling," which speeds up the estimate of the number of cells or particles that are randomly distributed within a tissue. The principle of square sampling is subdivision of a biopsy into at least 100 squares of the same size using a measuring ...
Andreoni G - - 1997
A new method is presented for the quantification of cell viability based on densitometry with computerized image analysis. Neuronal cells were stained with crystal violet and densitometric analysis was performed with an IBAS 2.0 image analyzer (Kontron/ Zeiss), using specially implemented dedicated software which integrates the optical density of the ...
Tortorello M L - - 1997
The antibody-direct epifluorescent filter (Ab-DEFT) technique was evaluated as a rapid alternative to the most probable number (MPN) method for enumeration of artificially inoculated Listeria monocytogenes in ready-to-eat packaged salads and other fresh vegetables. Ab-DEFT was performed by homogenization of food in mesh-lined Stomacher bags, followed by prefiltration of homogenate ...
Harkacz O M OM - - 1997
The purpose of this study was twofold: 1) to determine if the oral rehydration fluid Gatorade could serve as a suitable temporary storage medium for maintenance of periodontal ligament (PDL) cell viability on avulsed teeth and 2) to determine if fat content is related to maintenance of cell viability when ...
Jansen M W - - 1997
Fetal cells are present in the circulation of pregnant women and can be isolated using density gradient centrifugation and magnetic cell sorting. In the present study, maternal cell preparations were depleted for CD45- and CD14-positive cells and enriched for CD71-positive cells. The number of fetal nucleated cells was determined using ...
Lode H N - - 1997
A sensitive assay was developed for the detection of neuroblastoma cell contamination in CD34+ selected and unseparated peripheral blood stem cells (PBSC) used for autologous transplantation in stage 4 neuroblastoma patients. Specifically, we established a non-radioactive nested cDNA-PCR (nPCR) for detection of tyrosine hydroxylase (TH) gene expression combined with anti-disialoganglioside ...
Redenbach D M - - 1997
The size discrepancy between leukocytes [white blood cells (WBCs)] and pulmonary capillaries requires WBCs to deform. We investigated the persistence of this deformation on cells leaving the capillary bed and the role played by the cytoskeleton. Isolated rabbit lungs were perfused in situ via the pulmonary artery with effluent fractions ...
Joux F - - 1997
The direct viable count method first described by Kogure et al. (Can. J. Microbiol. 25:415-420, 1979) was improved by using an antibiotic cocktail instead of nalidixic acid alone. We screened 100 marine isolates from two coastal areas for their sensitivities to five replication-inhibiting antibiotics, including four quinolones (nalidixic, piromidic, and ...
Iles-Mann J - - 1997
The Cell Dyn 3500 (CD 3500) Haematology Analyser (Abbott Diagnostics, Lane Cove, NSW, Australia) has been evaluated to determine the reliability of the differential and white cell suspect flagging in a population of patients with known haematological abnormalities. The evaluation included the assessment of white cell differential parameters in addition ...
Arnutti P - - 1997
The study on quality control of automated blood cell analyzers, Technicon H*1 and Coulter MAXM by using three separately self-prepared control cells was extensively investigated. The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by glutaraldehyde, and control red cells from normal and thalassemic patients ...
Flint S H - - 1997
The use of the Malthus conductance growth analyser for the detection of Streptococcus bovis attached to stainless steel surface was evaluated. A comparison between the results from acridine orange epifluorescence direct counts, swab recovery viable count and conductance estimates of attached cell concentrations, based on calibrations for planktonic cells, showed ...
van Oostenbrugge R J - - 1997
OBJECTIVE: To determine the effect of the use of polylysine-coated (PLC) slides for cytocentrifugation on the morphology and number of cells in cerebrospinal fluid (CSF) and its effectiveness in improving the diagnostic sensitivity of the cytologic examination of CSF. STUDY DESIGN: We assessed the morphology and numbers of cells in ...
Hedreen J C - - 1997
We commemorate the one hundredth anniversary of the publication of a pioneering paper on cell counting, by Gaule and Lewin. Their paper describes a new method for counting cells in tissue sections. First they found the mean number of cell profiles per cell by examining 50 selected cells in serial ...
Tommy Norberg
The note provides a method for obtaining simultaneous confidence intervals for any combination of cell probabilities of a multinomial distribution. Large sample size is assumed.
< 12 13 14 15 16 17 18 19 20 21 22 >