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Results 801 - 850 of 1657
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Gao F B - - 1997
During vertebrate development, many types of precursor cell divide a limited number of times before they stop and terminally differentiate. It is unclear what limits cell proliferation and causes the cells to stop dividing when they do. The stopping mechanisms are important as they influence both the number of differentiated ...
Andersen C Y - - 1997
OBJECTIVES: Fluorescent in situ hybridization of decondensed sperm cells was used to evaluate the effectiveness of separating X- and Y chromosome bearing sperm cells using a combination of Percoll and NycoPrep media. MATERIAL AND METHODS: Semen samples from seven healthy men were divided into three equal aliquots. One aliquot served ...
Theocharous P - - 1997
The evaluation of contaminating breast cancer cells in hematopoietic grafts is of considerable importance for monitoring the efficiency of purging procedures. We report a comparison of three systems for the in vitro detection and enumeration of metastatic breast cancer cells. Breast cancer cells from established cell lines were mixed with ...
Paape M J - - 1997
Migration of neutrophils into mammary tissue provides the first immunological line of defense against bacteria that penetrate the physical barrier of the teat canal. Evasion of neutrophil defenses provides an opportunity for invading bacteria to become established. Depletion of neutrophils results in a dramatic increase in susceptibility to intramammary infection. ...
Sitar G - - 1997
BACKGROUND AND OBJECTIVE: Fetal erythroblasts in maternal circulation represent a valuable source of fetal cell material which can be obtained with non-invasive procedures that do not endanger the fetus. Physical separation techniques have been invaluable in the isolation and characterization of different cells. There are basically two principles that have ...
McFee A F - - 1997
Procedures are described for the in vitro culture of human lymphocytes, which have been concentrated by density gradient centrifugation, and for a modified slide-making technique for the fixed cells. The method yields improved percentages of mitotic cells which are largely synchronized at harvest. Controlled placement of fixed cells on slides ...
Connor C G - - 1997
PURPOSE: The number of goblet cells on the inferior bulbar conjunctiva increases in response to daily wear contact lens use. We conducted a study to examine whether daily wear soft contact lenses worn on a 2-week disposable schedule would also induce the same goblet cell response. METHODS: Twenty-eight subjects who ...
Sternberg C - - 1997
Detection of very low light levels arising from individual cells of the naturally bioluminescent bacterium Vibrio fischeri as well as from a luminescence-marked Pseudomonas putida strain was achieved by the aid of two different camera systems. Using a liquid nitrogen-cooled slow-scan CCD (charge-coupled device) camera were able to detect single-cell ...
Tanke H J - - 1996
This paper describes the use of automated microscopy to detect fetal erythroblasts in maternal blood. The technology is based on the following approach: (1) the use of centrifugal cytology for the preparation of monolayers; (2) simultaneous staining of fetal hemoglobin (immunoalkaline phosphatase) and chromosome sequences (FISH); (3) multi-mode microscopy to ...
Manion K - - 1996
An unusual population of high side scatter, low nucleic acid dye binding, dim CD45 cells was found in aged blood samples stained with the ProCOUNT reagent. Cell surface staining showed that these cells have the surface phenotype of neutrophils. However, they have decreased expression of several surface antigens, bind annexin ...
Mesker W E - - 1996
We investigated the feasibility of automated counting of in situ hybridization signals (ISH) in interphase cells isolated from paraffin embedded prostate tissue. In total, 34 specimens from 7 patients with prostate cancer were stained with probes specific for the centromeric regions of chromosomes Y, 1, 7, 8, 10, and 15, ...
Lo Y M - - 1996
The bilateral trafficking of nucleated cells between the fetus and the mother was studied using polymerase chain reaction (PCR)-based systems sensitive enough to detect 1 target cell in 100,000 background cells. Sixty-six mother-baby pairs were recruited; maternal and cord blood samples were collected at delivery for DNA extraction. Cell trafficking ...
Jones A R - - 1996
Short-term, within one 24-hour day (diurnal period) within-person changes of the principal blood count analytes in healthy subjects were studied at three major institutions. The results from each test site were indistinguishable and were therefore combined to make a database of 96 healthy subjects. Analytical imprecision of each analyte was ...
Cai Y - - 1996
Sputum analysis is a useful technique for the study of airway inflammation. In asthma, dithiothreitol (DTT) is used to disperse cells from surrounding mucus; however, the applicability of these processing methods to cystic fibrosis (CF) sputum is unknown. In order to compare two methods for processing sputum of patients with ...
Steele C D - - 1996
Many questions remain about the feasibility of using fetal cells from maternal blood for prenatal diagnosis. Although recently there has been more focus on clinically relevant methods, many studies have been performed using blood drawn after invasive procedures, and over a wide range of gestational ages. For methods to be ...
Zwerner R K - - 1996
A method is presented in which specific cells can be rapidly separated from whole blood by the use of antibody labelled dense particles utilizing gravity as a means of separation. This method specifically removes the targeted cells with minimal non-specific cell loss. The method is simple to perform and rapid, ...
Johnson M - - 1996
As part of an effort to develop a suspension-culture perfusion-based process with high flow rate without the fouling and antibody retention inherent to filter-based cell-separation devices, we have evaluated and contributed to the development of the Centritech Lab centrifuge for the perfusion culture of hybridoma cells in protein-free medium. Culture ...
Okamoto Y - - 1996
Density gradient centrifugation using Ficoll-Conray solution was attempted to isolate viable canine transmissible sarcoma (CTS) cells. The viability of isolated tumor cells increased from about 50% to > 90%, and the yield of CTS cells was > 50% with over 99% purity, in when an isolation solution density of 1.05 ...
Nagatomo H - - 1996
Quantitative comparisons between serum components and somatic cells in 202 bovine quarter milks collected at 3 dairy farms were conducted. Detection of serum components was carried out by an immunological technique, and cell counts were calculated by Breed's method. A high correlation (r = 0.77) between cell counts and gamma-globulin ...
Van Hoogmoed L - - 1996
OBJECTIVE: To characterize, in mares, changes in peritoneal fluid that occurred within the first 7 days after routine foaling. DESIGN: Prospective observational trial. ANIMALS: 15 mares. PROCEDURE: Abdominocentesis was performed within 10 days before foaling and again 12 hours, 3 days, and 7 days after each horse foaled. Data recorded ...
Micale M A - - 1996
We report a case of mosaic trisomy 20, the most common autosomal mosaicism identified in amniocytes, ascertained in a woman referred for amniocentesis because of abnormal ultrasound at 18.1 weeks' gestation which revealed short femurs and nuchal thickening. Metaphase analysis of 98 clones revealed 47,XY, +20 in 96 cells (98 ...
Yang T T - - 1996
We describe an acid phosphatase assay for determination of cell growth based on quantification of cytosolic acid phosphatase activity. The assay is based on the hydrolysis of the p-nitrophenyl phosphate by intracellular acid phosphatases in viable cells to produce p-nitrophenol. For all cell types examined, absorbance of p-nitrophenol at 405 ...
Morgante M - - 1996
On 700 milk samples from single half udders of Comisana ewes, somatic cell count (SCC) and differential cell count (DCC) were determined, using a Fossomatic 90 cell counter (Foss Electric, Denmark) (SCCF) and milk smears stained with May Grünwald-Giemsa (DCCS). SCC and DCC were also determined with modified KOVAH SYSTEM ...
Stokes B O BO Biological Research and Development Department, Wescor, Inc., Logan, Utah 84321, - - 1996
To determine the absorption time required to give complete cell recovery in cytocentrifugation. A simplified model for cell recovery was outlined that relates cell recovery to the relative magnitude of cell sedimentation velocity (SV) and fluid velocity (FV) during cytocentrifugation. The recovery of blood leukocytes was measured at various relative ...
O'Shea D G - - 1996
A semiautomatic image analysis method has been developed to characterize the morphology of the dimorphic yeast Kluyveromyces marxianus var. marxianus (formerly fragilis) NRRLy2415 undergoing alcoholic fermentation of cheese whey permeate. The method is capable of separating cells into six defined categories, varying from simple ovoid yeast cells to branched mycelial ...
Coe C L - - 1996
To evaluate possible long-term effects of exposure to monoaminergic drugs, blood and cerebrospinal fluid (CSF) samples were collected from adolescent monkeys that had been treated with desipramine and fluoxetine in infancy. This evaluation focused on the number and type of leukocytes in CSF as a reflection of cell trafficking in ...
Köpf I - - 1996
We here present a simplification of the entire procedure for preparing formalin-fixed, paraffin-embedded tissue to be used for FISH-analysis. The steps for deparaffinisation and disintegration of the tissue to produce intact cell nuclei in a monodispersed suspension are detailed as well as the hybridisation steps. The procedure results in a ...
Leach D N DN - - 1996
The efficiency of a current cell washing device for removing tumor cells from bovine blood was examined under laboratory conditions. In the in-vitro laboratory, anticoagulated bovine blood was seeded with known numbers of immunocytochemical stained human malignant epithelial cells (KB) grown in culture. The blood was subjected to cell washing. ...
Mohler W A - - 1996
We have developed a spectrophotometric assay for cell number in suspensions of tissue culture cells. For each cell type tested, absorbance between 650 and 800 nm is linearly dependent upon cell density over a 50-fold range and is independent of the color or composition of the medium in which cells ...
Handy L M - - 1996
BACKGROUND: Intestinal neutrophils can be studied by radiolabelling techniques and by cytology of whole-gut lavage fluid. Our aim was to evaluate the use of a biochemical test for the presence of these cells in whole-gut lavage fluid. METHOD: Whole-gut lavage was performed by having the patients drink a polyethylene-glycol-electrolyte solution; ...
McAndrews P T - - 1996
Although other methods of meniscus repair enhancement are being investigated (e.g., growth factors, synthetic matrices, stem cells), the results are too preliminary to warrant speculation about their potential for clinical use. Repair of the white-white meniscal tear remains an enigma. Although repair enhancement techniques, such as fibrin clot and synovial ...
Hofseth L J - - 1996
Epidemiological studies suggest an association between chronic inflammation and increased risk for cancer, although the mechanism underlying this relationship is unresolved. In the present study, we test the hypothesis that DNA damage is induced in the epithelium of tissues during such inflammation by products of activated inflammatory cells. Individuals on ...
Stelwagen K - - 1996
OBJECTIVES: To examine whether once daily milking (ODM) increases somatic cell count (SCC) and causes changes in the proportion of macrophages, lymphocytes, or neutrophils in milk, and to examine whether ODM causes mammary secretory cell damage. DESIGN: During 3 successive periods, cows were milked twice daily (days 1 through 4), ...
van de Rijke F M - - 1996
With the advent of in situ hybridization techniques for the analysis of chromosome copy number or structure in interphase cells, the diagnostic and prognostic potential of cytogenetics has been augmented considerably. In theory, the strategies for detection of cytogenetically aberrant cells by in situ hybridization are simple and straightforward. In ...
Pizzichini E - - 1996
Sputum examination is being used increasingly as a noninvasive method to assess airway inflammation. Expectorated sputum has variable contamination with saliva. Methods of processing have included the selection of portions of the sample considered to be representative of pulmonary origin versus use of the whole specimen, which is confounded by ...
Vorauer K - - 1996
A new assay to measure cell proliferation by turbidimetric evaluation of cultures in specially designed microtiter plates was set up. The IL-2-dependent proliferation of CTLL-2 cell line was used as a model. The novel microtiter plate detection system, the General Cell Screening System (GCSS) was used for the evaluation of ...
Fernández-Astorga A - - 1996
Escherichia coli O25:H-42 was selected to study the effect of pre-treatments on the enumeration of direct viable cells from milk samples. Before and after inducing cell elongation by cellular division inhibitors, three pre-treatments for milk-filtration were used. One involved a pre-treatment with trypsin (1.5 min at 50 degrees C), addition ...
Ren'e Van Oostrum,De Berg ...
In this paper we show how to traverse a subdivision and to report all cells, edges and vertices, without making use of mark bits in the structure or a stack. We do this by performing a depth-first search on the subdivision, using local criteria for deciding what is the next ...
Zuck T F - - 1996
The Food and Drug Administration will regulate cord blood cells stringently as a distinct category of somatic cells. The FDA has declared that cord blood cell products are subject to licensure and will require a claimed exemption for an investigational new drug (IND) to undertake clinical trials. This regulatory approach ...
Wilkerson C L - - 1996
OBJECTIVE: To systematically evaluate morphologic differences in iris stroma that contribute to clinically perceptible differences in iris color, using immunohistochemical identification of stromal melanocytes and fluorescence microscopy. METHODS: Paraffin-embedded sections from 51 human irides were stained with S100a and fluorescein isothiocyanate. Cells were counted and scored as melanocytes or other. ...
Jiang X - - 1996
Morphological changes of Vibrio parahaemolyticus from rods to spheres took place after a culture was subjected to starvation at a wide range of temperatures. Scanning electron micrographs revealed that starved spherical cells gradually developed a rippled cell surface with blebs and an extracellular filamentous substance adhesive to the cell surface. ...
Osterhout M L - - 1996
Precise determination of reticulocytes (young red blood cells) containing fetal hemoglobin (Hb F), F-reticulocytes, is important for assessment of the efficacy of drugs such as hydroxyurea and butyrate in elevating the levels of Hb F in patients with sickle cell disease (SCD) and beta-thalassemia. We developed a reliable and easily ...
Bianchi D W - - 1996
Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescence-activated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a "model' male ...
Zahniser D J - - 1996
An automated device has been developed to prepare a wide variety of cytology preparations. Using the device, cells collected in suspension are mildly dispersed and transferred to a glass slide using filter-transfer technology. The cell density on the slide is controlled by the instrument. A companion product, the solution used ...
van Wijk I J - - 1996
OBJECTIVE: Fetal cells circulate in the maternal blood during early pregnancy. Because these cells are rare, noninvasive prenatal diagnosis from fetal cells can be achieved only after efficient enrichment procedures. Our aim was to develop a two-step enrichment procedure to isolate trophoblast cells from 20 ml of peripheral blood. STUDY ...
Thompson A B - - 1996
The method of preparation of bronchoalveolar lavage fluid (BALF) for cytological examination can significantly affect the results of cellular quantitation. Investigations have shown that cytocentrifugation leads to an underestimation of the number of lymphocytes and membrane filter preparation to an underestimation of the number of neutrophils. As a simple alternative ...
Visser M J - - 1996
For in vitro evaluation of functional properties of endothelial cells seeded on synthetic vascular prostheses accurate and reproducible quantification of cells is mandatory. Comparison of these properties with those resulting from other studies requires correlation of the functional parameters to reliably counted cell numbers. The accuracy of methods of quantification ...
Edelman M J - - 1996
OBJECTIVES: Intraoperative autotransfusion of shed blood is widely utilized in surgery. However, several studies have raised concern about the transmission of tumor cells during oncologic procedures. We compared the ability of a leukocyte depletion filter (RC-400; LDF) to a standard red blood cell filter (SBF) to remove tumor cells derived ...
Wong H H - - 1996
The settling characteristics of cell debris and inclusion bodies prior to, and following, fractionation in a disc-stack centrifuge were measured using Cumulative Sedimentation Analysis (CSA) and Centrifugal Disc photoSedimentation (CDS). The impact of centrifuge feedrate and repeated homogenisation on both cell debris and inclusion body collection efficiency was investigated. Increasing ...
Isaacson L - - 1996
1. Using cyclic AMP to stimulate perfused tsetse fly Malpighian tubules bathed in SO42- Ringer frequently causes an immediate but transient peak in transtubular potential (Vt), before stabilisation of Vt at an increased value. 2. These transients were investigated by monitoring the associated changes in cable properties and current&shy;voltage (I/V) ...
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