Search Results
Results 601 - 650 of 1768
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Ferrer Elena - - 2004
We retrospectively evaluated the effectiveness of nevirapine-containing regimens in 118 naive patients initiating highly active antiretroviral therapy with CD4 cell counts S 200 cells/jl. After 24 months, 51% of patients continued nevirapine, 43 and 83% had viral loads < 50 copies/ml by intent-to-treat and on-treatment analyses, and a mean increase ...
Tefferi Ayalew - - 2004
Laboratory methods to detect both FIP1L1-PDGFRA and c-kit D816V mutations were combined with immunomagnetic cell separation to study the extent of clonal involvement by both myeloid and lymphoid cells in 3 patients with systemic mastocytosis associated with eosinophilia. The results suggested an early stem cell origin for the FIP1L1-PDGFRA mutation.
Taylor Theresa B - - 2004
BACKGROUND: Since the sequencing of the human genome has been finished, microgenomics has been booming, employing highly sophisticated, high-throughput platforms. But these mainly chip-based methods can only generate biologically relevant data if the samples investigated consist of homogeneous cell populations, in which no unwanted cells of different specificity and/or developmental ...
Szaniszlo Peter - - 2004
BACKGROUND: Most biological samples are cell mixtures. Some basic questions are still unanswered about analyzing these heterogeneous samples using gene expression microarray technology (MAT). How meaningful is a cell mixture's overall gene expression profile (GEP)? Is it necessary to purify the cells of interest before microarray analysis, and how much ...
Kakinuma Hiroaki - - 2004
BACKGROUND: Methylmalonic aciduria (MMA) is divided into two groups according to cobalamin dependency, and this classification is important for treatment. Unfortunately, there has been no rapid and reliable method for the evaluation of cobalamin dependency. METHODS: [14C]-propionate incorporation into intact cells in the presence of either media alone or media ...
van Rijt Leonie S - - 2004
Mouse models of allergic asthma are increasingly used to study the immunopathology of this complex disorder. The degree and type of airway inflammation is often studied by determination of differential cell counts on cytospins of bronchoalveolar lavage fluid (BALF) cells stained with May-Grünwald Giemsa, in which the separation of eosinophils ...
Simpson J L - - 2004
Excessive salivary contamination of induced sputum samples prevents the satisfactory examination of lower airway inflammation. The effects of salivary contamination on different sputum fluid phase measures and the levels of salivary contamination preventing analysis are not defined. The present study sought to examine this by investigating the effect of increasing ...
Dukes K A - - 2004
OBJECTIVE: To test the hypothesis that a delay in initial fetal cell enrichment processing of maternal blood samples (defined as the time between blood draw and the initial density gradient centrifugation step) compromises the ability to recover fetal cells, we performed a randomized comparison of immediate (within 4 hours of ...
Gussin Hélène A Elicha - - 2004
OBJECTIVES: We hypothesized that fetal cells in maternal blood that do not respond to hematopoietic culture conditions represent endothelial cells. We investigated whether endothelial progenitor cells of fetal origin may be selected from maternal blood on the basis of their expression of CD133 or CD105 and expanded in culture. METHODS: ...
Veress B - - 2004
BACKGROUND: The number of intraepithelial lymphocytes in the duodenum was determined 30 years ago, the suggested normal upper limit being 40 lymphocytes per 100 epithelial cells. METHODS: Duodenal mucosa was analysed from 18 healthy individuals and 56 consecutive patients biopsied because of epigastralgia (17 cases), diarrhoea (10 cases), oesophagitis (10 ...
Aybar Buket - - 2004
The aim of this study was to evaluate beta-tricalcium phosphate (beta-TCP Cerasorb Curasan-Germany) graft materials on specific parameters of rat osteoblast activity in vitro. Primary culture osteoblastic cells were isolated from neonatal rat calvaria by sequential collagenase digestion. To analyze the effect of biomaterials on cell proliferation, cell numbers and ...
Edlund B - - 2004
The effect of sample aging was studied on 80 blood samples collected from in-house patients within 2 hours before aspiration into the Cell-Dyn 4000 hematology analyzer. The first aspiration defined the zero-time value, and the samples were stored at ambient room temperature (23 degrees C) and reanalyzed at 12, 24, ...
Toft Gunnar - - 2004
OBJECTIVES: To quantify semen quality in the Greenlandic population. STUDY DESIGN: A cross-sectional study including recently proven fertile men from four regions including nine municipalities and one settlement in Greenland. METHODS: The samples were analysed for sperm cell concentrations and motility using standard methods. RESULTS: In total 201 semen samples ...
Ahn Yeonghee - - 2004
To better understand microbial community in sulfur-based nitrate removal process, comparative molecular analyses were performed with the biofilm formed on sulfur particles that were obtained from different bed-heights of a laboratory-scale reactor employed for the process. Microbial community in the reactor showed vertical heterogeneity in terms of total cell count ...
Dobbe J G G - - 2004
BACKGROUND: The deformability of red blood cells (RBCs) is of great importance for the conservation of oxygen delivery in the microcirculation. Even a small fraction of rigid cells is considered to harm the exchange of respiratory gases. Techniques that measure RBC deformability often provide an indication of the mean deformability. ...
Arpagaus M - - 2004
BACKGROUND: JACIE Standards (FACT Standards in the USA) have been implemented in Europe since 1999. An on-site accreditation inspection took place at our center in January 2004. The purpose of this work was to develop a real-time process/quality control system meeting the JACIE Standards for HPC release. METHODS: Data from ...
Westengard James - - 2004
Method comparison and calibration are important for clinical hematologists and for manufacturers of new methods and instruments. Automation of the white blood cell (WBC) differential count poses unique problems in this regard because the reference method is a microscopic manual cell count of 200 WBCs. A highly variable reference method ...
Makrydimas George - - 2004
OBJECTIVE: Prenatal paternity testing can be performed following invasive prenatal diagnosis with amniocentesis or CVS. Coelocentesis is a new technique that could be used as an alternative method early in the first trimester of pregnancy. The aim of this study is to investigate the potential use of the DNA extracted ...
Gerstner A O H - - 2004
BACKGROUND: Eosinophilia within nasal polyps is often taken as a criterion for adjuvant medical treatment postoperatively such as topical steroids. OBJECTIVE: This study was performed in order to validate a new technique for objective quantification of eosinophilia by using laser scanning cytometry (LSC), to compare these results with manual scoring ...
Heyrman M - - 2004
The thermodynamic properties of condensed phases, i.e. the activities of components, can be determined from partial pressures measured by the Knudsen cell mass spectrometric method. Improvements in accuracy and yield of this method are obtained with the use of twin cells, an idea proposed in the 1960s. The multiple cell ...
Oliszewski Rubén - - 2004
Mastitis is a general term that refers to the inflammation of the mammary gland. It is the most common illness in dairy farms and it has different causes, mainly a great number of germs that infect the gland. These infectious diseases induce gross variations in milk composition, reflected by physical, ...
Björnheden Tom - - 2004
OBJECTIVE: Fat cell size is a fundamental parameter in the study of adipose tissue metabolism, because it markedly influences the cellular rates of metabolism. Previous techniques for the sizing of adipocytes are often complicated or time-consuming. The aim of this study was to develop a new, computerized method for rapid ...
Pacheco C C - - 2003
Epifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 microm filters, referred as 'ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter ...
Mason J Bohannon - - 2003
A white blood cell count (WBC) of >50000 cell/mm(3) from a knee aspirate with >or=80% polymorphonuclear cells (PMNCs) is suggestive of infection. This study sought to determine if these same criteria were applicable when interpreting aspirates from a total knee. Of 440 revision total knee arthroplasties, 86 patients had preoperative ...
Tsantes Argirios - - 2003
A recently developed immunocytochemical technique in HbF-cell counting was assessed by an objective evaluation method. The basic principle of this method is the preparation of aliquots with predetermined HbF-cell (target) values. These aliquots serve as control samples to standardize the HbF-cell measurements by the new immunocytochemical technique, which uses the ...
Guillou Sandrine - - 2003
Assessment of yeast viability by plate counts, ATP determination and FUN-1 viability staining was performed to study sublethal injury of Saccharomyces cerevisiae cells submitted to low-amperage electrolysis. Lethal effects of electrolysis were confirmed by all methods, demonstrated by the decrease in viable counts observed during electrolysis. FUN-1 viability staining and ...
Osswald Kerstin - - 2003
Buccal cells serve as targets to assess oral exposures. We have refined isolation methods to characterise yield, viabilities, types of cells and baseline levels of genetic damage. Buccal cells were isolated from mouthwashes of 27 volunteers. They were characterised microscopically and different methods (using antibody-labelled magnetic beads, filtration and gradient ...
Hughes K J - - 2003
OBJECTIVE: To compare a fast Romanowsky cytological stain (Diff-Quik) and Leishman's stain for the detection of mast cells in samples from the lower airways of racehorses, and to compare the proportion of mast cells and eosinophils in the total inflammatory cells in tracheal aspirate (TA) with those in paired bronchoalveolar ...
Carter Janell - - 2003
White cells are a critical functional element circulating in blood. This study sheared fresh whole bovine blood in stainless steel and polymeric capillary tubes of various lengths and diameters. Flow rate was constant, resulting in a range of exposure times and shear stresses. White cell count, cell integrity (trypan blue ...
Henkel R - - 2003
The presence of excess leucocytes in the semen has been associated with male infertility. According to the WHO, concentrations of more than 106 leucocytes ml-1 are considered as leucocytospermia, indicating genital tract infections. Up to now, no consensus has been achieved on how leucocytes should be quantified in semen. Using ...
Loukas Constantinos G - - 2003
BACKGROUND: Semiquantitative evaluation and manual cell counting are the commonly used procedures to assess positive staining of molecular markers in tissue sections. Manual counting is also a laborious task in which consistent objectivity is difficult to achieve. Recently, image analysis has been explored, but the studies reported were limited to ...
Le Roux Yves - - 2003
Relationships between Quarter Milk Cell Count (QMCC) and Tank Milk Cell Count (TMCC) with milk biochemical and technological parameters in milk and dairy products were investigated. All parameters measured were affected by the increase of TMCC and QMCC between 0 and 600 000 cells/mL. The variable effect of lactation stage ...
Pérez-Ruiz M - - 2003
The ability of the RD (rhabdomyosarcoma) and MRC-5 cell-lines to detect enteroviruses in 33 clinical samples (cerebrospinal fluid, stools and throat swabs) was evaluated. The samples had previously tested enterovirus-positive by traditional tube-culture and had been frozen after their initial processing. By traditional tube-culture, 100 and 85 % of samples ...
Higgins Richard - - 2003
Optical true-time-delay devices based on the White cell can be divided into two general types: polynomial cells, in which the number of delays that can be obtained is related to the number of times m that a beam bounces in the cell raised to some power, and exponential cells, in ...
Whiteley Andrew S - - 2003
Total and active cell counts within soil samples were determined by culture-independent methods using flow cytometry and preparative Nycodenz gradient centrifugation. Whole cells were purified from soil cores and total extractable cell counts assessed by SYBR Green II fluorescence, while active cell counts were determined by 5-cyano-2,3-ditolyl tetrazolium chloride reduction ...
Zhao J - - 2003
BACKGROUND: Controversial results have been reported regarding the effect of anaesthetics on superoxide anion production during the respiratory burst (RB) of polymorphonuclear cells (PMN). The differences could be caused by the cell preparation methods and the aim of this study was to compare two techniques. METHODS: RB activity was measured ...
Wigg Alan J - - 2003
The assessment of cell concentration and viability of freshly isolated hepatocyte preparations has been traditionally performed using manual counting with a Neubauer counting chamber and staining for trypan blue exclusion. Despite the simple and rapid nature of this assessment, concerns about the accuracy of these methods exist. Simple flow cytometry ...
Bischoff F Z - - 2003
Isolation and analysis of intact fetal cells in maternal blood is an attractive method of non-invasive prenatal diagnosis; however, detection levels are not optimal. The poor sensitivity and inconsistent recovery of fetal cells is compounded by small numbers of circulating fetal cells and loss of fetal cells during enrichment procedures. ...
Zingsem Jürgen - - 2003
BACKGROUND: Umbilical cord blood processing with standard centrifugation techniques is performed in open systems and results in varying cell and volume recoveries. STUDY DESIGN AND METHODS: Forty umbilical cord blood donations were randomly assigned to processing either with a microprocessor-controlled cell separator equipped with closed disposables or with a manual ...
Trindade H - - 2003
The distribution of leucocyte subpopulations in platelet concentrates (PC) derived from pre-storage filtered platelet-rich plasma (PRP), the cell suspension obtained by reverse filter washing and the post-filtered PC, were monitored by immunophenotyping analysis using CD3, CD20 and CD33. Leucocyte activation analysis with the CD11b marker revealed that this molecule is ...
Swolin B - - 2003
The morphological appearance of blood cells has an established association to clinical conditions. A novel system, DiffMaster Octavia for differential counting of blood leukocytes, has been evaluated. The system consisted of a microscope, 3-chip color charge coupled device (CCD) camera, automated motorized stage holder, electronic hardware for motor and light ...
Ozkan A - - 2003
Flotation is one of the most important physicochemical processes for mineral separations and other recovery operations. Flotation machines have been developed since the beginning of the 19th century and are still under intensive research and development. The cell we devised is a combination of the Canadian column flotation cell and ...
Bouvier Thierry - - 2003
Abstract Fluorescence in situ hybridization (FISH) is widely used to describe bacterial community composition and, to a lesser extent, to describe the physiological state of cells. One of the limitations of the technique is that the effectiveness of the detection of target cells appears to vary widely. Here, we present ...
Shiono Hiroyuki - - 2003
A compact bench-top model of the centrifuge enables continuous cell separation based on density differences. The apparatus holds a small separation disk equipped with a circular channel (8 mL capacity) separated by a septum. A set of isotonic Percoll media with different densities is continuously introduced at one terminal and ...
Lim R - - 2003
To assess the adequacy of current decontamination methods for the Goldmann tonometer in the context of variant Creutzfeldt-Jakob disease (vCJD). Reusable Goldmann tonometer prisms were used to perform applanation tonometry on different groups of patients. Following tonometry, retained materials were collected from the tonometer prism head and examined using cytological ...
Conner Barrett D - - 2003
OBJECTIVE: To compare the results of pleural fluid analysis for WBC counts and differential cell counts as follows: (1) counting performed manually vs that performed by an automated cell counter; (2) cells collected in different types of specimen containers; and (3) cell counts performed at 4 and 24 h postthoracentesis. ...
Rose Laura J - - 2003
The survival of two strains of Yersinia pestis (avirulent A1122 and virulent Harbin) on the surfaces of four materials was investigated. Viability was evaluated with epifluorescence microscopy by using the metabolic stain cyanoditolyl tetrazolium chloride and plate counts. Small numbers of cells suspended in phosphate buffer survived 2 to 4 ...
Rupp R - - 2003
Records from 94,445 and 45,499 French Lacaune dairy ewes in first and second lactations, respectively, were used to estimate genetic parameters for somatic cell scores. Somatic cell count data came from an extensive recording scheme and sample testing that began in 1999 using the flocks enrolled in the official milk ...
English Only,Statistics New Zealand
Introduction Tables of business magnitude data, which are the main output from business surveys, have a relatively high disclosure risk. Business populations tend to be skewed, with many small and medium-sized businesses and just a few very dominant businesses. This means that, for sampling efficiency, the large businesses are usually ...
Dosogne H - - 2003
Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk. We have developed a flow cytometric differential leukocyte count method for low SCC milk. The procedure consists of 1) 1.5 ml of ...
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