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Results 551 - 600 of 1943
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Tahar Aissa Jamel - - 2009
Conclusions: This study showed that macrophages, B cells, and T cells occurred at different frequencies, localizations, and times after acute laser tympanic membrane perforation. Immunological cells were most prevalent in the proliferative mass adjacent to the annulus region. The cellular immunological response in freshly perforated eardrums was unexpectedly aggressive. Objectives: ...
Lambrecht Bernd - - 2009
BACKGROUND: A fully automated single-tube assay with tubes (BD TruCOUNT, BD Biosciences) for absolute counting of residual cells in freshly prepared plasma by flow cytometry was developed (BD Plasma Count). STUDY DESIGN AND METHODS: The nucleic acid dye thiazole orange stains white blood cells (WBCs). The monoclonal antibodies anti-CD41a-peridinin chlorophyll ...
Rosenberg Tara L - - 2009
OBJECTIVES: We undertook (1) to measure the cell density within the lamina propria of the neonatal vocal folds and (2) to examine changes in cell density in the lamina propria with increasing gestational age of the neonatal vocal folds. METHODS: Intact neonatal larynges were obtained from fresh cadaveric specimens. Hematoxylin ...
Halasa T - - 2009
Milk, fat, and protein loss due to a new subclinical mastitis case may be economically important, and the objective of this study was to estimate this loss. The loss was estimated based on test-day (TD) cow records collected over a 1-yr period from 400 randomly selected Dutch dairy herds. After ...
Zhao Yongxi - - 2009
Preparation of calibration standards for cell enumeration is critical in characterizing the performance of any method or apparatus intended for recovering rare cells. Diluting a cell suspension serially is prone to statistical sampling errors as the cell suspension becomes more dilute, whereas transferring and injecting cells individually into a diluent ...
Klingbeil Ma Fátima Guarizo - - 2009
The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be also useful for other parts of the human body. Many researchers still use controversial methods; therefore it was evaluated ...
Sonak, S.
The crystal violet microplate adhension assay was modified to evaluate bacterial adhesion to metal and non-metal surfaces. Both viable cell count and the absorbance of the crystal violet stained cells attached to aluminium increased over the period of ineubation. There was a highly significant positive linear relationship between crystal violet ...
Lehec Sharon C - - 2009
For clinical hepatocyte transplantation, cells need to be prepared in a sterile GMP environment. Strict regulations are in place that set the standard for this environment that cells are prepared in. These regulations control all aspects of the environment. In the United Kingdom, the laboratory must have a licence from ...
Vistejnova Lucie - - 2009
OBJECTIVES: Determination of cell numbers is a crucial step in studies focused on cytokinetics and cell toxicity. The impedance-based analysis employing electronic sensor array system xCELLigence System allowing label-free dynamic monitoring of relative viable adherent cell amounts was compared with the most utilized methods for relative quantification of viable cell ...
McAtee Peter A - - 2009
Cell size is a structural component of fleshy fruit, contributing to important traits such as fruit size and texture. There are currently a number of methods for measuring cell size; most rely either on tissue sectioning or digestion of the tissue with cell wall degrading enzymes or chemicals to release ...
Cortopassi-Laurino M - - 2009
In order to analyze the pollen resources used by the orchid bee Euglossa annectans, samples of larval provisions from cells under construction were taken from 12 different trap nests (wooden boxes) on Santa Catarina Island, southern Brazil. The 43 samples collected between 2002 and 2005 represented all months except December. ...
Balbo Andrea - - 2009
The analytical ultracentrifuge (AUC) is a powerful biophysical tool that allows us to record macromolecular sedimentation profiles during high speed centrifugation. When properly planned and executed, an AUC sedimentation velocity or sedimentation equilibrium experiment can reveal a great deal about a protein in regards to size and shape, sample purity, ...
Halama Niels - - 2009
Determining the correct number of positive immune cells in immunohistological sections of colorectal cancer and other tumor entities is emerging as an important clinical predictor and therapy selector for an individual patient. This task is usually obstructed by cell conglomerates of various sizes. We here show that at least in ...
Forero Manuel G MG NeuroDevelopment Group, School of Biosciences, University of Birmingham, Birmingham, - - 2009
Development, cancer, neurodegenerative and demyelinating diseases, injury, and stem cell manipulations are characterised by alterations in cell number. Research into development, disease, and the effects of drugs require cell number counts. These are generally indirect estimates, because counting cells in an animal or organ is paradoxically difficult, as well as ...
Catling S - - 2008
The intra-operative blood loss of 50 consecutive gynae-oncology patients undergoing surgery for endometrial, cervical or ovarian cancer was cell salvaged and filtered. In each case blood samples were taken from the effluent tumour vein, a central venous line, the cell saver reservoir, the cell salvage re-transfusion bag after processing but ...
Yao Kazuo - - 2009
OBJECTIVE: To examine local inhibition of Th2 cell infiltration as the basis for demonstrating the superior clinical effect of trichloroacetic acid (TCA) treatment for allergic rhinitis. METHODS: Mirror-image sections of the inferior turbinate mucosae of both sides were obtained from 26 patients who underwent TCA treatment on one side because ...
León Agathe - - 2009
BACKGROUND: CD4+ T cell recovery dynamics were analysed during the 'on treatment' periods in structured therapy interruption (STI) as well as the long-term immune reconstitution with highly active antiretroviral therapy (HAART) after finishing STI. METHODS: One hundred and twenty HIV-1-infected patients on successful HAART were randomized to receive for 2 ...
Decordier Ilse - - 2009
Micronuclei (MN) frequencies in peripheral blood lymphocytes have been used worldwide as a biomarker of chromosomal damage for genotoxicity testing and biomonitoring studies. Automation of MN analysis would provide faster and more reliable results with minimizing subjective MN identification. We developed an automated facility for the scoring of the in ...
Riggio Oliviero - - 2008
AIM: To evaluate the accuracy of automated blood cell counters for ascitic polymorphonuclear (PMN) determination for: (1) diagnosis, (2) efficacy of the ongoing antibiotic therapy, and (3) resolution of spontaneous bacterial peritonitis (SBP). METHODS: One hundred and twelve ascitic fluid samples were collected from 52 consecutive cirrhotic patients, 16 of ...
Lehto T - - 2008
CELL-DYN Ruby is a new automated hematology analyzer suitable for routine use in small laboratories and as a back-up or emergency analyzer in medium- to high-volume laboratories. The analyzer was evaluated by comparing the results from the CELL-DYN((R)) Ruby with the results obtained from CELL-DYN Sapphire . Precision, linearity, and ...
Kadivar Maryam M Department of Pathology and Laboratory Medicine, Iran University of Medical Sciences, Tehran, Iran. - - 2008
To compare the argyrophilic nucleolar organizer region (AgNOR) count of cells collected from the normal buccal mucosa of cigarette smokers, opium addicts and nonsmokers. Exfoliative cytologic smears of buccal mucosa from 25 smokers, 25 addicts and 25 nonsmokers were stained for AgNORs according to the Ploton's method. The AgNOR count ...
Densmore Lezlie - - 2008
Using fluorochrome-labeled probes for the X and Y chromosomes, fluorescence in situ hybridization (FISH) was used to test for the presence of male cells in the milk of women breastfeeding male babies. After breastfeeding, the women wiped one breast and collected a test sample. Wiping efficiency was evaluated in 2 ...
Warnatz Klaus - - 2008
BACKGROUND: Common variable immunodeficiency (CVID) comprises heterogeneous antibody deficiency disorders. To classify this heterogeneous syndrome, clinical as well as immunologic parameters have been combined. Flowcytometric analysis of circulating T and B lymphocyte subpopulations has become an important tool in this endeavor of disease classification. METHODS: Multicolor flowcytometric analysis of circulating ...
Li Chenhong - - 2008
This article describes a simple whole cell formation technique that the author invented in teaching and experiments. The implementation of the invented technique is a syringe with a hole and slot. With the newly invented technique, novices will shorten their learning curve and veterans will increase their success rate. The ...
Seppo Antti - - 2008
OBJECTIVE: As fetal cells can be indisputably identified through detection of Y FISH signals, we utilized an automated microscopy system developed to identify and enumerate cells bearing X and Y FISH signals. We further investigated the potential of fetal hemoglobin expression as a gender independent marker for automated identification of ...
Singletary Morgan L - - 2008
Bronchoalveolar lavage (BAL) by means of bronchoscopy is a diagnostic tool frequently used for clinical and research purposes in nonhuman primates. Although many institutions use this procedure, the technique is not standardized. One technical aspect that can vary is the method by which fluid is recovered. The purpose of this ...
Kumar Ashok - - 2008
The analysis and separation of cells including rare cell populations from a heterogeneous population has become an important tool in scientific research and medicine. The last two decades have seen tremendous progress in the field of cell separation, as a result of which various methods have been available which utilize ...
Bai Yang - - 2008
The present study was aimed at finding an effective method to isolate and purify the subtype of type A spermatogonial stem cells (SSCs) in juvenile rats. Testes from 9-days-old rats were used to isolate germ cells by using two-step enzymatic digestion. The expression of c-kit in the testes of the ...
Schreiner N M S - - 2008
BACKGROUND: Histopathology is widely used for the diagnosis of inflammatory bowel disease in dogs. Variations in lesions and unavailability of uniform grading systems limit the usefulness of histologic examination. HYPOTHESIS: CD3 cell numbers in chronic enteropathies of dogs correlate with clinical activity of the disease and with severity of histopathologic ...
M. Saravanan, A. Noorul ...
Scheduling problem in a Cellular Manufacturing System (CMS) environment is treated as group scheduling problem, which assumes that all parts in a part family are processed in the same cell and no inter-cellular transfer is needed. In a typical CMS environment, however, there could be some exceptional parts, which need ...
Stenqvist Ann-Christin - - 2008
PROBLEM: The uniqueness of the human placenta cannot be replaced by animal models. In vitro studies are compulsory to elucidate the biology of human placenta and require isolation and purification of villous trophoblasts, which can be used in molecular and functional studies. Constant improvement in the isolation technique is required ...
El-Manzalawy Yasser Y Artificial Intelligence Laboratory, Iowa State University, Ames, IA 50010, USA. - - 2008
The identification and characterization of B-cell epitopes play an important role in vaccine design, immunodiagnostic tests, and antibody production. Therefore, computational tools for reliably predicting linear B-cell epitopes are highly desirable. We evaluated Support Vector Machine (SVM) classifiers trained utilizing five different kernel methods using fivefold cross-validation on a homology-reduced ...
Alonso-Sáez Laura - - 2008
We collected surface samples in Franklin Bay (Western Arctic) from ice-covered to ice-free conditions, to determine seasonal changes in the identity and in situ activity of the prokaryotic assemblages. Catalysed reported fluorescence in situ hybridization was used to quantify the abundance of different groups, and combined with microautoradiography to determine ...
Amalfitano Stefano - - 2008
Efficient detachment and purification of bacterial cells associated with streambed sediments are required in order to quantify cell abundance and to assess community composition through the application of epifluorescence microscopy techniques. We applied chemical (i.e., sodium pyrophosphate and polysorbate) and physical treatments (i.e., shaking and sonication), followed by Nycodenz density ...
Kerr, Geoff
The dichotomous choice approach to contingent valuation is extremely popular, but obtains very little information from each respondent and is therefore inefficient. Multiple-bounded dichotomous choice approaches are more efficient in theory, but theoretical gains are not always obtained in practice. The multiple-bounded dichotomous choice approach also yields internally inconsistent responses. ...
Doughty Michael J - - 2008
PURPOSE: The aim of this study was to compare two methods of assessments of the coefficient of variation (COV) of endothelial cell area. METHODS: A single image (Topcon SP-2000P specular microscope) was obtained from the central region of the corneal endothelium of 45 healthy white (Norwegian) individuals, aged from 24 ...
Frundzhyan Valery G - - 2008
Somatic cell count (SCC) in milk is considered to be a valuable indicator of cow mastitis. For assessment of SCC in milk, the bioluminescent assay based on determination of ATP from somatic cells ([ATPsom]) in milk was proposed earlier. However, this assay is still not widely used in practice owing ...
Funato Michinori - - 2008
The effectiveness of a white-cell reduction filter to deplete contaminated leucocytes in preventing the harmful effects of transfusion is evident. However, several complications associated with a white-cell reduction filter have been identified. We report the first case of anaphylactoid reactions caused by a white-cell reduction filter with a positively charged ...
Hawkins Michelle G - - 2008
OBJECTIVE: To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and SpO(2) values obtained during BAL in healthy rabbits. ANIMALS: 9 rabbits. PROCEDURES: Bronchoscopic BAL of left and right caudal lobar bronchi (LB2 and RB4) was performed with 3 mL of sterile saline (0.9% NaCl) solution; SpO(2) was ...
Koess Cordula - - 2008
Subclinical mastitis is a costly disease and its diagnosis is difficult. Besides the somatic cell count (SCC) and bacteriology, the differential inflammatory cell count (DICC) is a meaningful tool for mastitis detection. As microscopy is very subjective because of the low number of events to be counted, flow cytometry has ...
Fu Lung-Ming - - 2008
Using a simple and reliable isotropic wet etching process, we fabricated a microflow cytometer in which cells/particles are concentrated in the center of the sample stream using a 2-D hydrodynamic focusing technique and an microweir structure. Having focused the cells/particles, they are detected and counted using a LIF method. The ...
tpcoutinho
<i>Melosira jürgensii</I><br>Two cells in girdle view. Permanent slide (Hasle, 1978)
Holland Nina - - 2008
The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary ...
Waard, Menno de
Test day data before drying off and after calving were collected for 220 Dutch dairy cows in a study on the effect of vitamin E supplementation on udder health. The cows divided over 5 different Dutch dairy farms were split in two groups and given either a high vitamin E ...
Li Zhixin - - 2008
Highly enriched, pure populations of pluripotent mouse embryonic stem (mES) cells are a prerequisite to downstream experimental manipulations. However, the existing preplating method does not allow complete removal of co-cultured mouse embryonic fibroblast (MEF) feeder cells. The primary objective of the current investigation was to develop and validate a rapid, ...
Butch Anthony W - - 2008
Manual hemacytometer cell counting of body fluids is labor-intensive and requires skilled testing personnel. We performed a multicenter evaluation of the Iris iQ200 automated microscopy analyzer Body Fluids Module (Iris Diagnostics, Chatsworth, CA) and compared 350 iQ200 body fluid cell counts with manual hemacytometer cell counts. Within-run imprecision, expressed as ...
Wiwanitkit Viroj - - 2008
Bird flu or avian flu is a new emerging Infectious disease. The H5N1 virus has not only affected the fowls but also the human beings. Severe disease with high mortality in humans in Vietnam and Thailand are reported. This mini-study was undertaken in order to document the Impact of bird ...
Barbulovic-Nad Irena - - 2008
We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple ...
Wang Kelong - - 2008
In this paper, an open-tubular capillary cell affinity chromatography (OT-CAC) method to enrich and separate target cells is described. Open tubular capillaries coated with anti-CD4, anti-CD14, or anti-CD19 antibodies were used as affinity chromatography columns to separate target blood cells. Cells were eluted using either shear force or bubbles. Bubbles ...
Prophet Technologies Pty Ltd
This work is a collection of tips and tricks that may make using Microsoft Excel 2003 a little easier and interesting to use. This work describes how to easily create a series of numbers, view all the formulas on a worksheet, highlight a cell based on special conditions, use conditional ...
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