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Results 501 - 550 of 1849
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Li Zhixin - - 2008
Highly enriched, pure populations of pluripotent mouse embryonic stem (mES) cells are a prerequisite to downstream experimental manipulations. However, the existing preplating method does not allow complete removal of co-cultured mouse embryonic fibroblast (MEF) feeder cells. The primary objective of the current investigation was to develop and validate a rapid, ...
Butch Anthony W - - 2008
Manual hemacytometer cell counting of body fluids is labor-intensive and requires skilled testing personnel. We performed a multicenter evaluation of the Iris iQ200 automated microscopy analyzer Body Fluids Module (Iris Diagnostics, Chatsworth, CA) and compared 350 iQ200 body fluid cell counts with manual hemacytometer cell counts. Within-run imprecision, expressed as ...
Wiwanitkit Viroj - - 2008
Bird flu or avian flu is a new emerging Infectious disease. The H5N1 virus has not only affected the fowls but also the human beings. Severe disease with high mortality in humans in Vietnam and Thailand are reported. This mini-study was undertaken in order to document the Impact of bird ...
Barbulovic-Nad Irena - - 2008
We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple ...
Wang Kelong - - 2008
In this paper, an open-tubular capillary cell affinity chromatography (OT-CAC) method to enrich and separate target cells is described. Open tubular capillaries coated with anti-CD4, anti-CD14, or anti-CD19 antibodies were used as affinity chromatography columns to separate target blood cells. Cells were eluted using either shear force or bubbles. Bubbles ...
Prophet Technologies Pty Ltd
This work is a collection of tips and tricks that may make using Microsoft Excel 2003 a little easier and interesting to use. This work describes how to easily create a series of numbers, view all the formulas on a worksheet, highlight a cell based on special conditions, use conditional ...
Pitol Dimitrius Leonardo - - 2008
The aim of this study was to present a morphological description of the leukocytes of Phrynops hilarii turtles according to the seasonal distribution of these cells and to show their replacement in the blood circulation using a radioautographic method. Five animals of both sexes weighing 600-1200 g were used. The ...
Panda Anita - - 2008
PURPOSE: To evaluate patients with ocular surface squamous neoplasia (OSSN) on treatment with topical mitomycin C (MMC) with regard to changes in corneal thickness and endothelial count. DESIGN: Prospective, nonrandomized, interventional series. METHODS: In this prospective evaluation, 25 patients with OSSN with different clock hours of involvement (range, one to ...
Li Yanfeng - - 2008
In the effective index method, which models photonic crystal fibers by means of a step-index waveguide analogy, two critical parameters are the effective index of the cladding and the effective core radius. It has been shown that the use of an effective core radius as a function of the relative ...
Maria Antónia Sampayo
<i>Ceratium kofoidii</I><br>Two cells. Natural Sample.
Maria Antónia Sampayo
<i>Dinophysis sacculus</I><br>Divinding cell. Natural sample.
Yang, Liying
Detection of rare, circulating tumor cells (CTCs) in peripheral blood is a potential prognostic/diagnostic tool in oncology. The use of immunomagnetic cell separation has been shown to improve the target cell purity and thus detection sensitivity. In this dissertation, a repeatable enrichment process including a flow through immunomagnetic cell separation ...
Khoory, Haifa
[Truncated abstract] The collection of buccal cells is common practise in the epidemiological and forensic science. Unlike venipuncture collection of blood; it is a safer, non-invasive method for collection of biological material. The methods by which these cells are collected from the inner cheek of an individual and stored are ...
R. Asadpour
This study was conducted to determine the levels and relationship between Lacto Peroxidase (LP) activity and somatic cell count (SCC) for diagnosis subclinical mastitis in early lactation of dairy cows. Foremilk samples were obtained from quarters of 80 cows (August 2007 up to February 2008). Any cows had not evidence ...
Li, H.
The technique of maintaining a proper crystalline ratio in microcrystalline silicon (μc-Si:H) layers along the thickness direction by decreasing the H2 dilution ratio during deposition (H2 profiling) was introduced by several laboratories while optimizing either n–i–p or p–i–n μc-Si:H cells made by PECVD. With this technique a great increase in ...
Zerfaß, T.
The exact segmentation of nucleus and plasma of a white blood cell (leukocyte) is the basis for the creation of an automatic, image based differential white blood cell count(WBC). In this contribution we present an approach for the according segmentation of leukocytes. For a valid classification of the different cell ...
Wilk Anna - - 2008
This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a "density cushion". The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was ...
Doughty Michael J - - 2008
PURPOSE: To compare the outcome measures from two different methods for estimating the coefficient of variation (COV) in cell area for human corneal endothelia. METHODS: A single non-contact specular micrograph was obtained from the central region of the corneal endothelium of 100 healthy non-contact lens-wearing white European subjects, aged from ...
Goldberg Stanley - - 2008
This chapter covers the various methods of mechanical cell disruption and tissue homogenization that are currently commercially available for processing minute samples (<1 ml) to larger production quantities. These mechanical methods of lysing do not introduce chemicals or enzymes to the system. However, the energies needed when using these "harsh" ...
Ferrier G A - - 2008
The mechanical behavior of cells offers insight into many aspects of their properties. We propose an approach to the mechanical analysis of cells that uses a combination of electromanipulation for stimulus and capacitance for sensing. To demonstrate this approach, polystyrene spheres and yeast cells flowing in a 25 mumx100 mum ...
Pisoni G - - 2008
Nocardia spp. are an uncommon cause of mastitis, and outbreaks have typically been reported in dairy farms with poor hygienic and management conditions. The outbreak described herein involved a dairy farm with 43 lactating cows that, after a long period with low bulk milk somatic cell counts (<180,000 cells/mL), experienced ...
Doughty Michael J - - 2008
BACKGROUND: Little has been published on the reliability of estimates of the coefficient of variation (COV) in cell area for human corneal endothelia. The present study compares two methods. METHODS: A non-contact specular micrograph (Topcon SP-2000P) was obtained from the central region of the corneal endothelium of 20 healthy myopic ...
Jouannic Jean-Marie - - 2008
Coelocentesis may represent the ideal technique for early prenatal diagnosis. This study aimed to quantify the number of cells in coelomic fluid and to investigate the feasibility of interphase fluorescence in-situ hybridization (FISH) in uncultured coelomic cells for chromosomes X and Y in 12 samples of 0.4-0.8 ml of coelomic ...
McKeown Peter - - 2008
We describe a method for isolating nuclei from cultured Arabidopsis cells. The same method can be used to further isolate nucleoli. Cell walls are first digested to yield protoplasts, which are purified by flotation on a Percoll gradient. Mechanical homogenisation is used to release nuclei, or with more homogenisation, nucleoli. ...
Liu Tianming - - 2008
Quantification of cells is a critical step towards the assessment of cell fate in neurological disease or developmental models. Here, we present a novel cell detection method for the automatic quantification of zebrafish neuronal cells, including primary motor neurons, Rohon-Beard neurons, and retinal cells. Our method consists of four steps. ...
Gakamsky Anna - - 2008
The detection of chemotaxis-related changes in the swimming behavior of mammalian spermatozoa in a spatial chemoattractant gradient has hitherto been an intractable problem. The difficulty is that the fraction of responsive cells in the sperm population is very small and that the large majority of the cells, though non-responsive, are ...
Korohoda Włodzimierz - - 2008
In this paper, we discuss the application of various methods of cell electrophoresis in research into cell surface properties (analytical methods), and the separation of uniform cell subpopulations from cell mixtures (preparative methods). The emphasis is on the prospects of the development of simplified and versatile methodologies, i.e. microcapillary cell ...
McLaren J W - - 2008
Cell density in the corneal stroma is typically determined by counting the number of bright objects, presumably keratocyte nuclei, in images from clinical confocal microscopy. We present a program that identifies bright objects and counts those that most likely represent cells. Selection variables were determined from 125 normal corneas with ...
Su Ting-Wei TW Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA. - - 2008
We illustrate that by recording under-sampled diffraction patterns of cells at different illumination angles, we can achieve high-throughput on-chip characterization of a heterogeneous cell solution over an ultra large volume of approximately 5 ml. This platform, termed multi-angle LUCAS, is especially promising for cost-effective point-of-care cell counting applications.
Tang Yitai Y Department of Biochemistry and Cell Biology; Rice University; Houston, Texas - - 2008
One of the simplest examples of a complex behavior is the aggregation of solitary Dictyostelium discoideum amoebae to form a 20,000-cell fruiting body. A field of starving amoebae first breaks up into territories. In each territory, the cells form a spider-like pattern of streams of cells. As part of a ...
Lucy D - - 2007
Modem forensic techniques allow DNA to be extracted from ever decreasing amounts of cellular material. Low copy number (LCN) profiling enables the production of STR profiles from small numbers of cells. Moreover, methods such as laser micro-dissection enables forensic scientists to potentially isolate individual cells for PCR. The DNA derived ...
Bechhofer, R.
A Curtailed Sequential Procedure for Subset Selection of Multinomial Cells
Okada Yoshinori - - 2007
Human diploid cells are more susceptible to oxidative stress at late passage than at early passage, presumably because of the decrease in cellular-reduced glutathione (GSH) concentration. Water-soluble protein (WSP) from broad beans scavenges free radicals. The effects of WSP on the glutathione system were examined in PDL 20 (early passage) ...
Nigro Kelly - - 2007
The purpose of this study was to compare four cell block (CB) methods in the setting of nongynecologic ThinPrep (TP) specimens. 48 CBs were prepared from 12 nongynecologic TP specimens using the following CB methods: (1) Inverted filter sedimentation (IFS); (2) Thrombin method; (3) Albumin method; (4) Simple sedimentation. Each ...
E Michalska Anna - - 2007
To realize their potentials, embryonic stem (ES) cells must be maintained in optimal culture conditions that preserve their pluripotency and self-renewal capacity. Mouse embryonic fibroblasts (MEFs) are used to prepare a feeder cell layer that supports the growth of ES cells and the quality of feeders is crucial for the ...
Shimakita Tomonori - - 2007
An apparatus for the non-culture method (NCM) of microbial cell count was formerly developed and named a bioplorer. The bioplorer NCM is based on the double staining of cells with 4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and the automatic analysis of their fluorescent microscopic images. Viable cells can be ...
Rabal Obdulia - - 2007
Cell-based methods and the diversity integral criterion (a distance-based technique) are commonly used approaches for assessing the diversity of collections of compounds in terms of space coverage. The main deficiency with cell-based methods is the arbitrariness of cell boundaries which leads to edge effects or cluster artifacts, i.e., situations in ...
Guo Junming - - 2007
BACKGROUND: Increasing the sensitivity and specificity of detecting circulating carcinoma cells of patients with hepatocellular carcinoma (HCC) is very important for monitoring recurrence. GOAL: To establish a novel method of detecting circulating carcinoma cells. STUDY: For method development, 3 sets of controls using HCC cell line HepG2 cells were used. ...
Kubota M - - 2007
Quantitative PCR can be an effective method for identifying the bacteria causing mastitis. However, PCR detection is hampered by the presence of inflammatory somatic cells. To eliminate this problem, we attempted to establish methods that allow the effective separation of bacterial cells from somatic cells in mastitis milk with amino-silica. ...
Wang Kelong - - 2007
A simple device for the separation of cells by phenotype is described. Cells are separated/isolated using capture antibodies on a glass chip. Unlike other "sandwich" type assays, the readout is performed without labels using transmission microscopy, simplifying cell enumeration. T and B lymphocytes from cell culture or whole blood were ...
Escobar-Herrera Jaime - - 2007
The standardization of a plaque assay for feline calicivirus in Crandell Reese feline kidney cells using carboxymethyl-cellulose as an overlay medium is described in this report. This methodology gives comparable counts as compared to the standard assay, and prevents monolayer roll over and peel off, as well as easy medium ...
Kissling Grace E - - 2007
Until recently, the in vivo erythrocyte micronucleus assay has been scored using microscopy. Because the frequency of micronucleated cells is typically low, cell counts are subject to substantial binomial counting error. Counting error, along with inter-animal variability, limit the sensitivity of this assay. Recently, flow cytometric methods have been developed ...
Hyodo Maki - - 2007
OBJECTIVES: We have determined the number of fetal nucleated cells and the concentration of cell-free fetal DNA in parallel in the same maternal blood samples either before or after delivery, and studied the relationship between these two. METHODS: Venous blood samples were taken at four points around delivery from ten ...
Gadelha A P R - - 2007
In this work, we describe a semiautomated computer method to evaluate the activity of a common drug solvent, dimethyl sulfoxide (DMSO), on in vitro Giardia lamblia-host cell interaction. To compare the number of intestinal cells (IEC-6) and the adhered trophozoites over a specific area in control and treated coculture, a ...
Namsolleck Pawel - - 2007
Fluorescence in situ hybridization (FISH), which is used for the enumeration of bacteria in various ecosystems including the human intestinal tract, has several limitations. One of the major problems encountered is the uneven distribution of bacterial cells on the slide surface, which increases the coefficient of variation between repetitions and ...
Porra Valérie - - 2007
BACKGROUND: As an alternative to the cumbersome Kleihauer-Betke test (KBT), flow cytometry represents a powerful method for the identification and quantification of fetal red blood cells (RBCs) in maternal circulation. STUDY DESIGN AND METHODS: The aim of this study was to evaluate the Fetal Cell Count kit (IQ Products), an ...
Kim Youngho - - 2007
To reduce the problem of cell loss due to adhesion, one of the basic phenomena in microchannel, we proposed the droplet-based magnetically activated cell separator (DMACS). Based on the platform of the DMACS-which consists of permanent magnets, a coverslip with a circle-shaped boundary, and an injection tube-we could collect magnetically ...
Bonnet Noël - - 2007
We propose two methods for characterizing the spatio-temporal behavior of cell populations in culture. The first method, image auto-correlation microscopy (IACM), allows us to characterize the variation in the number of objects as a function of time, thus enabling the quantification of the clustering properties of cell populations to be ...
Wu X-L - - 2007
A Bayesian analysis via Markov chain Monte Carlo methods extending the simultaneous and recursive model of Gianola and Sorensen (2004) was proposed to account for possible population heterogeneity. The method was used to infer relationships between milk yield and somatic cell scores of Norwegian Red cows. Data consisted of test-day ...
Mohamed Hisham - - 2007
Isolation of fetal cells from maternal circulation is the subject of intense research to eliminate the need for currently used invasive prenatal diagnosis procedures. Fetal cells can be isolated using magnetic-activated cell sorting or fluorescence-activated cell sorting, however no technique to specifically isolate and use fetal cells for genetic diagnosis ...
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