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Park Ki E - - 2013
The cardiovascular cell therapy network was developed by the National Heart, Lung and Blood Institute to design and conduct clinical trials to advance the field of cardiovascular (CV) cell-based therapy. The Cardiovascular Cell Therapy Network successfully completed three clinical trials involving approximately 300 subjects across five centers and six satellites. ...
Huang Chenyu - - 2013
It has long been thought that the effectiveness and efficiency of physical therapy would improve if our understanding of the cell biology/biochemistry that participates in mechanics could be improved. Traditional physical therapy focuses primarily on rehabilitation, but recent developments in mechanobiology that illuminated the effects of physical forces on cells ...
Jacob M M Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, - - 2012
Tissue remodeling is critical during development and wound healing. It also characterizes a number of pathologic conditions, including chronic inflammation, fibrosis and cancer. It is well appreciated that reactive stromal cells play critical roles in these settings. However, understanding of the mechanisms involved in the differentiation of reactive stromal cells ...
Spence Heather J HJ The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, - - 2012
The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been ...
Pajoohesh-Ganji Ahdeah - - 2012
Goblet cells are terminally differentiated cells secreting mucins and antibacterial peptides that play an important role in maintaining the health of the cornea. In corneal stem cell deficiency, the progenitor cells giving rise to goblet cells on the cornea are presumed to arise from differentiation of cells that migrate onto ...
Wang Lei-Lei - - 2012
Inflammation plays important roles in the development of diabetic retinopathy (DR). How Müller cells contribute to DR-related inflammation remains unclear. We hypothesized that under diabetic conditions, elevated histone acetylations in Müller cells contribute to the inflammatory response. In this study, significantly increased histone acetylations, elevated histone acetyltranferases levels, and decreased ...
Nedosekin Dmitry A - - 2012
In vivo photoacoustic (PA) and fluorescence flow cytometry were previously applied separately using pulsed and continuous wave lasers respectively, and positive contrast detection mode only. This paper introduces a real-time integration of both techniques with positive and negative contrast modes using only pulsed lasers. Various applications of this new tool ...
Hu Wenqi - - 2012
A novel hydrogel microrobot made of poly(ethylene glycol) diacrylate (PEGDA) is reported. This disk-shaped microrobot is optothermally actuated by laser-induced bubbles. A pulsed laser is used to smoothly actuate an 80-μm-diameter bubble microrobot at up to 320 μm s(-1). A single microrobot or a pair of microrobots working in cooperation ...
Witherden Deborah A - - 2012
γδ T cells respond rapidly to keratinocyte damage, providing essential contributions to the skin wound healing process. The molecular interactions regulating their response are unknown. Here, we identify a role for interaction of plexin B2 with the CD100 receptor in epithelial repair. In vitro blocking of plexin B2 or CD100 inhibited γδ ...
Pakhomova Olga N - - 2012
Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells and media exposed to 300-ns PEF (1-13 kV/cm) was assessed ...
Turner William S - - 2012
The packaging and delivery of cells for cardiac regeneration has been explored using a variety biomaterials and delivery methods, but these studies often ignore one or more important design factors critical for rebuilding cardiac tissue. These include the biomaterial architecture, strength and stiffness, cell alignment, and/or incorporation of multiple cell ...
Schlie Sabrina - - 2012
Topographical cues have a significant impact on cell responses and by this means, on the fabrication of innovative implant materials. However, analysis of cell-topography interactions in dependence of the surface feature dimensions is still challenging due to limitations in the fabrication technology. Here, we introduce surface structuring via picosecond laser ...
Karnas Diana - - 2012
Intrinsically photosensitive retinal ganglion cell (ipRGCs) types can be distinguished by their dendritic tree stratification and intensity of melanopsin staining. We identified heavily stained melanopsin positive M1 cells branching in the outermost part of the inner plexiform layer (IPL) and weakly melanopsin positive M2 cells branching in the innermost layer ...
Cruz de Carvalho Ricardo - - 2012
Background and AimsThe aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage.MethodsIntracellular ROS production ...
Bragheri F - - 2012
The main trend in optofluidics is currently towards full integration of the devices, thus improving automation, compactness and portability. In this respect femtosecond laser microfabrication is a very powerful technology given its capability of producing both optical waveguides and microfluidic channels. The current challenge in biology is the possibility to ...
Emes Yusuf - - 2012
To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the ...
Joshi Sagar D - - 2012
Sheets of embryonic epithelial cells coordinate their efforts to create diverse tissue structures such as pits, grooves, tubes, and capsules that lead to organ formation. Such cells can use a number of cell behaviors including contractility, proliferation, and directed movement to create these structures. By contrast, tissue engineers and researchers ...
Leopold Philip L - - 2012
Wound healing and cancer metastasis share a common starting point, namely, a change in the phenotype of some cells from stationary to motile. The term, epithelial-to-mesenchymal transition (EMT) describes the changes in molecular biology and cellular physiology that allow a cell to transition from a sedentary cell to a motile ...
Lin Hsin-Yu - - 2012
Small molecule BODIPY dyes incorporating conjugated substituents at the β sites have been synthesized. Solution processed inverted bulk heterojunction cells were fabricated from the blends of the dyes and PC(71)BM. The cells exhibited very high open-circuit voltages (>0.9 V) and a high conversion efficiency of 3.22% has been achieved.
Schlingmann Barbara - - 2012
Connexin46 (Cx46), together with Cx50, forms gap junction channels between lens fibers and participates in the lens pump-leak system, which is essential for the homeostasis of this avascular organ. Mutations in Cx50 and Cx46 correlate with cataracts, but the functional relationship between the mutations and cataract formation is not always ...
Kopsachilis Nikolaos - - 2012
To investigate if human anterior lens capsule is a suitable substrate for the culture of primary human trabecular meshwork (HTM) cells. Trabecular meshwork cells derived from four human donors were seeded on anterior lens capsules that were prepared from the lenses of donor eyes. Cell morphology and viability were examined ...
Zhu Wei - - 2012
The capacity for tissue and organ regeneration in humans is dwarfed by comparison to that of salamanders. Emerging evidence suggests that mechanisms learned from the early phase of salamander limb regeneration-wound healing, cellular dedifferentiation and blastemal formation-will reveal therapeutic approaches for tissue regeneration in humans. Here we describe a unique ...
Doughty Michael J - - 2012
PURPOSE: The aim of this paper was to assess the morphological features of cells stained with rose Bengal along the human eyelid marginal zone (Marx's line). METHODS: Impression cytology (IC) specimens were taken using a Millicell-CM filter unit placed across Marx's line of the lower eyelid of 10 healthy male ...
Shan Zhanhai - - 2012
Gadd45a, the first well-defined p53 downstream gene, can be induced by multiple DNA-damaging agents, which plays important roles in the control of cell cycle checkpoint, DNA repair process and signaling transduction. Our previous findings suggested that Gadd45a maintains cell-cell adhesion and cell contact inhibition. However, little is known about how ...
Whitney Jon R - - 2012
Aim: Nanoparticle-enhanced photothermal therapy is a promising alternative to tumor resection. However, quantitative measurements of cellular response to these treatments are limited. This article introduces a Bimodal Enhanced Analysis of Spatiotemporal Temperature (BEAST) algorithm to rapidly determine the viability of cancer cells in vitro following photothermal therapy alone or in ...
Hasirci Vasif - - 2012
Aim: This article reports on studies conducted in the same laboratory on interactions between patterned substrates with different pattern dimensions and chemistries, and various types of cells. Materials & methods: In order to compare the influence of various parameters, bone marrow stromal cells, retinal pigment epithelial cells, human corneal stromal ...
Wikström Jonna - - 2012
Encapsulated human retinal pigment epithelial cell line ARPE-19 has been successfully used in experimental cell therapy of retinal degenerations and Parkinson's disease, but the long-term storage of encapsulated cells is still an unresolved question. Reconstitution of viable encapsulated cells from dry form would benefit the development of cell therapy products. ...
Lee Se-Jung - - 2012
Although interleukin-28A (IL-28A) is believed to have an antiviral effect, its role in tumor migration requires further examination. The present study was intended to verify the effect of IL-28A on the migration of UMUC-3 bladder cancer cells. IL-28A and its receptor IL-28AR1 mRNA were detected in UMUC-3 cells. Although exogenous ...
Doyle Wayne - - 2012
The popularity and prevalence of the energy beverages make it essential to examine the interactions between the ingredients and their effects on safety of these beverages. In this study, we used in vitro assays to examine the effects of two energy beverages on mesenchymal, epithelial and neuronal cells. Our results ...
Dumas Virginie - - 2012
The femtosecond laser processing enabled the structuring of six types of surfaces on titanium-6aluminium-4vanadium (Ti-6Al-4V) plates. The obtained hierarchical features consisted of a combination of microgrooves and oriented nanostructures. By adjusting beam properties such as laser polarization, the width of the microgrooves (20 or 60 μm) and the orientation of ...
Akram K M - - 2012
Clara cells participate in bronchiolar wound repair and regeneration. Located in the bronchioles they become activated during alveolar injury in idiopathic pulmonary fibrosis (IPF) and migrate into the affected alveoli - a process called alveolar bronchiolisation. The purpose of this migration and their role in alveolar wound repair is controversial. ...
Takagi Ryo - - 2012
Mouse 3T3 feeder layer has been utilized for epidermal and corneal epithelial cell culture to promote tissue-like cell stratification. However, the molecular mechanism underlying epithelial-feeder layer interactions remains poorly understood. Here, the feeder layer activity of six different mouse cell lines were examined in terms of the colony forming efficiency ...
Kuo Chien-Neng - - 2012
Bevacizumab, a recombinant humanized monoclonal antibody, binds vascular endothelial growth factor (VEGF) and inhibits its interaction with receptors found on endothelial cells. Bevacizumab has been increasingly used as an off-label treatment for exudative age-related macular degeneration (AMD). Whether or not bevacizumab is capable of arresting the growth of human retinal ...
Chen Peng - - 2012
PURPOSE: This paper aimed to determine the involvement of Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) in experimental murine corneal neovascularization (NV), and to study the effect of NHERF1 on the biological properties of HUVEC and related mechanisms. METHODS: Using loss- and gain-function, we investigated the biological effects of NHERF1 on ...
Yoo Jae-Keun - - 2012
We demonstrate a measurement apparatus to inspect spatial uniformity of quantum efficiency of solar cells using a beam projector. Deviation of irradiance from the used beam projector over the area of 1.5×0.8  m on the cell plane was flattened within ±2.6% through gray scale adjustment, which was originally about 200%. Scanning ...
Freire Vanesa - - 2012
Purpose: To compare the effects of three blood derivatives (Autologous Serum, AS; Platelet-Rich Plasma, PRP and serum derived from Plasma Rich in Growth Factors, PRGF) on a human corneal epithelial (HCE) cell line, in order to evaluate their potential as an effective treatment for corneal epithelial disorders.Methods: The concentrations of ...
Liu Wenjia - - 2012
Purpose: The photosensitive polyimide film, Durimide, is a common component of retinal prostheses; however, retinal cell response to Durimide has not been effectively studied This work assessed the in vitro biocompatibility of a retinal prosthesis containing platinum-electrode embedded Durimide film. Materials and methods: Biocompatibility evaluation assessed cytotoxicity, attachment, and proliferation ...
Gagnon Kenneth B - - 2012
Cell motility is dependent on a coordinated reorganization of the cytoskeleton, membrane recycling, and focal adhesion to the extracellular matrix. Each of these cellular processes involves re-distribution of cell water, which is facilitated by the transport of inorganic ions (with obligatory water movement). Scratch-wound healing assays of Wistar C6 glioblastoma ...
Wang Xiaokun - - 2012
Ceramic-derived materials have shown enhanced osteogenic potential for bone tissue engineering applications. Silica is the major component of bioglass, and titania, the oxide complex of titanium, has been found to enhance osteoblast differentiation. In this study, three groups of sol-gel-derived silica-titania fibrous meshes with precursor ratios of Ti:Si = 7:3, ...
Ramaesh T - - 2012
Matrix metalloproteinase-9 (MMP-9) secreted by corneal epithelial cells has a role in the remodelling of extracellular matrix and migration of epithelial cells. Elevated levels of MMP-9 activity in the ocular surface may be involved in the pathogenesis of corneal diseases. N-acetylcysteine (NAC) has been used to treat corneal diseases, including ...
Bertseva E - - 2012
We present the microrheological study of the two close human epithelial cell lines: non-cancerous HCV29 and cancerous T24. The optical tweezers tracking was applied to extract the several seconds long trajectories of endogenous lipid granules at time step of 1μs. They were analyzed using a recently proposed equation for mean ...
Watson Alastair J M - - 2012
Although epithelial cells are continuously shed from the surface of the intestine, the intestinal epithelium maintains the integrity of the epithelial barrier. This is achieved through a highly dynamic process involving reorganization of tight junction and adherens junction proteins. This process both ejects the cell from the epithelial monolayer and ...
Favicchio Rosy - - 2012
Detection of multiple fluorophores in conditions of low signal represents a limiting factor for the application of in vivo optical imaging techniques in immunology where fluorescent labels report for different functional characteristics. A noninvasive in vivo Multi-Spectral Normalized Epifluorescence Laser scanning (M-SNELS) method was developed for the simultaneous and quantitative ...
Julio Gemma - - 2012
PURPOSE.: To establish the main indicator of progressive corneal exposure to dry eye conditions in an animal model. METHODS.: Cell surface covered by microvilli (SCM), intercellular junctions (IJs), cell area, cell shape, and cell shade were measured in 988 epithelial corneal cells from rabbit eyes that were kept open for ...
Paszek Matthew J - - 2012
Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton ...
Ahn Mee Young - - 2012
J Oral Pathol Med (2012) Background:  Pheophorbide a (Pa) is a chlorine-based photosensitizer derived from an ethnopharmacological herb, and our group recently synthesized Pa by the removal of a magnesium ion and a phytyl group from chlorophyll-a. In this study, the effect of photodynamic therapy (PDT) with synthesized Pa was ...
Sugiura Kana - - 2012
1) Fluorescence spectrum at 298 and 40 K and absorption spectrum at 298 K of each cyanobacterial cell was measured by the single-cell confocal laser spectroscopy to study differentiation of cell pigments. 2) Fluorescence spectra of vegetative ( veg) and heterocyst ( het) cells of a filamentous cyanobacterium Nostoc sp. ...
Takagi Mutsumi - - 2012
Time-lapse analysis of phase shift using phase-shifting laser microscopy (PLM) revealed that the laser phase shifts of primary normal human prostate epithelial cells (PRECs) and human prostatic carcinoma epithelial cell line (PC-3) cells during the mitotic phase were markedly higher than those in the interphase. The phase shift of PC-3 ...
Li Wen-Bo - - 2012
PURPOSE. To investigate the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into retinal pigment epithelium (RPE) cells, and identify development-regulating microRNAs (miRNAs).METHODS. RPE cells were derived from hPESCs. The expression of markers and miRNA expression profiles during differentiation were studied by immunocytochemistry, real-time reverse transcription PCR (RT-PCR), ...
Campetelli Alexis - - 2012
Cell polarity plays a key role in regulating cell-cell communication, tissue architecture and development. Both internal and external cues participate in directing polarity and feedback onto each other for robust polarization. One poorly appreciated layer of polarity regulation comes from electrochemical signals spatially organized at the level of the cell ...
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