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Balbach Sebastian T ST Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, Münster, 48149, - - 2015
Nuclear transfer (NT) into mouse oocytes yields a transcriptionally and functionally heterogeneous population of cloned embryos. Most studies of NT embryos consider only embryos at predefined key stages (e.g., morula or blastocyst), that is, after the bulk of reprogramming has taken place. These retrospective approaches are of limited use to ...
Bogdani Marika M Matrix Biology Program, Benaroya Research Institute, 1201 Ninth Avenue, Seattle, WA, 98101, USA, - - 2014
Type 1 diabetes (T1D) results from progressive immune cell-mediated destruction of pancreatic β cells. As immune cells migrate into the islets, they pass through the extracellular matrix (ECM). This ECM is composed of different macromolecules localized to different compartments within and surrounding islets; however, the involvement of this ECM in ...
Mishima Takuya T Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, - - 2014
Peripheral T cells are in G0 phase and do not proliferate. When they encounter an antigen, they enter the cell cycle and proliferate in order to initiate an active immune response. Here, we have determined the first two cell cycle times of a leading population of CD4(+) T cells stimulated ...
Chen Hui H Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, State Key Laboratory of Medical Molecular Biology, Beijing, 100005, - - 2014
γδT cells function as sentinels in early host responses to infections and malignancies. Previously, we found ectopically expressed human MutS homologue 2 (hMSH2), recognized by γδT cells, triggered a γδ T cell-mediated cytolysis to tumor cells. However, the characteristics of hMSH2-specific γδ Τ cells are not fully understood. In this ...
Chaudhry Mohammed S MS Centre for Haematology, Imperial College London, Hammersmith Hospital, London W12 0NN, United - - 2014
CD1d is a nonpolymorphic, MHC class I-like molecule that presents phospholipid and glycosphingolipid Ags to a subset of CD1d-restricted T cells called invariant NKT (iNKT) cells. This CD1d-iNKT cell axis regulates nearly all aspects of both the innate and adaptive immune responses. Expression of CD1d on B cells is suggestive ...
Kaku Hiroaki H Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research, Manhasset, NY 11030; and - - 2014
Immune suppression by regulatory T cells and regulatory B cells is a critical mechanism to limit excess inflammation and autoimmunity. IL-10 is considered the major mediator of B cell-induced immune suppression. We report a novel mechanism for immune suppression through adenosine generation by B cells. We identified a novel population ...
Tsirigoti Amerssa A Department of Botany, Faculty of Biology, University of Athens, Panepistimiopolis, Athens, 157 84, - - 2014
Eurychasma dicksonii is one of the most common and widespread marine pathogens and attacks a broad spectrum of more than 45 brown algal species. The present study focuses on the mechanism used by the pathogen to attach on the host cell wall and force its way into algal cells. Ultrastructural ...
Sikalidis Angelos K AK Dana-Farber Cancer Institute, Harvard Medical School, Harvard Institutes of Medicine, Immunobiology Laboratory, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA, - - 2014
While proteins are critical for immunity, T-cells constitute a critical component of adaptive immunity by clearing cancerous cells among other abnormal cells. However, cancer cells exhibit a potential to escape T-cell control by employing mechanisms not completely delineated. Interesting work has investigated how certain amino acids affect the proliferation rate ...
Lv Mingming M Medicine, Immunology and Reproductive Biology - - 2014
Non-small-cell lung cancer (NSCLC) patients with malignant pleural effusion (MPE) have a short median survival time and the increased Treg cells. However, it is unclear whether some specific factors in MPE are involved in Treg cell recruitment in the progression of NSCLC. Here, we found that Treg cell population was ...
Wolter Daniel J DJ Department of Pediatrics, University of Washington, Seattle, Seattle, WA 98195, - - 2014
In this issue of Cell Host & Microbe, Hammer et al. (2014) show that distinct, slow-growing bacteria have better in vitro and in vivo growth and virulence when cocultured than in isolation. They provide evidence that the observed inter- and intraspecies "complementation" involves the intercellular exchange of metabolites.
Wratil Paul R PR Charite - Universitaetsmedizin Berlin, - - 2014
Due to its position at the outermost of glycans, sialic acid is involved in a myriad of physiological and pathophysiological cell functions such as host-pathogen interactions, immune regulation and tumor evasion. Inhibitors of cell surface sialylation could be a useful tool in cancer, immune, antibiotic, or antiviral therapy. In this ...
Mitxitorena Izaskun I Institut de Neurociènces, Department of Biochemistry and Molecular Biology, School of Medicine, Universitat Autònoma de Barcelona, Barcelona, - - 2014
T cells engage with antigen-presenting cells to form immunological synapses. These intimate contacts are characterized by the complex arrangement of molecules at the intercellular interface, which has been described as the supramolecular activation cluster (SMAC). However, due to T cells functioning without SMAC formation and the difficulties of studying these ...
Pfister Sandra S Institute of Pharmacology and Toxicology, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Winterthurerstrasse 190, CH-8057, Zurich, - - 2014
The olfactory epithelium (OE) of mice deficient in cystic fibrosis transmembrane conductance regulator (CFTR) exhibits ion transport deficiencies reported in human CF airways, as well as progressive neuronal loss, suggesting defects in olfactory neuron homeostasis. Microvillar cells, a specialized OE cell-subtype, have been implicated in maintaining tissue homeostasis. These cells ...
Laval Kathlyn K Department of Virology, Parasitology and Immunology, Ghent University, - - 2014
Equine herpesvirus type 1 (EHV-1) replicates in the epithelial cells of the upper respiratory tract and disseminates through the body via a cell-associated viremia in monocytic cells, despite the presence of neutralizing antibodies. However, the mechanism by which EHV-1 hijacks immune cells and uses them as 'Trojan horses' in order ...
Kongsbak Martin - - 2014
Background In vitro studies have shown that the active form of vitamin D3, 1¿,25-dihydroxyvitamin D3 (1,25(OH)2D3), can regulate differentiation of CD4+ T cells by inhibiting Th1 and Th17 cell differentiation and promoting Th2 and Treg cell differentiation. However, the serum concentration of 1,25(OH)2D3 is far below the effective concentration of ...
Schmeits P C J PC RIKILT Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE, Wageningen, The Netherlands, - - 2014
Transcriptomics in combination with in vitro cell systems is a powerful approach to unravel modes of action of toxicants. An important question is to which extent the modes of action as revealed by transcriptomics depend on cell type, species and study type (in vitro or in vivo). To acquire more ...
Rodriguez Saint-Jean S S Centro de Investigaciones Biológicas, CSIC, C/Ramiro de Maeztu 9, 28040, Madrid, - - 2014
In the present work, the establishment and biological characterization of a new cell line, SSP-9, derived from the pronephros of the Atlantic salmon Salmo salar, are reported. These cells grew well in Leibovitz's (L15) medium supplemented with 10% foetal calf serum at temperatures from 15 to 25° C, and they ...
Jiang Yi Y State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PR China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR - - 2014
In our previous study, we found that polymorphisms of the mpt64 gene in the Mycobacterium tuberculosis complex (MTBC) may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions. Based on the data, we further analyzed T/B cell epitope regions and non-epitope ...
Ovadya Yossi Y Department of Molecular Cell Biology, The Weizmann Institute of Science, 76100, Rehovot, - - 2014
The progression of physiological ageing is driven by intracellular aberrations including telomere attrition, genomic instability, epigenetic alterations and loss of proteostasis. These in turn damage cells and compromise their functionality. Cellular senescence, a stable irreversible cell-cycle arrest, is elicited in damaged cells and prevents their propagation in the organism. Under ...
Brodowska Katarzyna K Retina Service, Angiogenesis Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, MA, - - 2014
Recent studies suggest that the anti‑diabetic drug metformin may reduce the risk of cancer and have anti‑proliferative effects for some but not all cancers. In this study, we examined the effects of metformin on human retinoblastoma cell proliferation in vitro and in vivo. Two different human retinoblastoma cell lines (Y79, WERI) were ...
Rocha-Perugini Vera V Servicio de Inmunología; Hospital Universitario de la Princesa; Instituto de Investigación Sanitaria de la Princesa; Madrid, Spain; Vascular Biology and Inflammation Department; Centro Nacional de Investigaciones Cardiovasculares; Madrid, - - 2014
Nuclear lamins A/C control several critical cellular functions, e.g., chromatin organization, gene transcription, DNA replication, DNA damage responses, cell cycle progression, cell differentiation, and cell polarization during migration. However, few studies have addressed the role of lamins A/C in the control of the functions of immune cells. Recently, we have ...
Hendriks W K WK Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, 3584 CM Utrecht, The - - 2014
Equine embryos are cryopreserved by slow-freezing or vitrification. While small embryos (<300 μm) survive cryopreservation reasonably well, larger embryos do not. It is not clear if slow-freezing or vitrification is less damaging to horse embryos. To compare the type and extent of cellular damage suffered by small and large embryos ...
Olofinsae Samson Ayodeji SA School of Health, Sport and Bioscience, University of East London, United - - 2014
To investigate the effect of phorbol 12-myristate 13-acetate (PMA) and formyl-methionyl-leucyl-phenylalanine (FMLP) on oxygen consumption of differentiated and non-differentiated immune cell lines by retinoic acid and calcitriol treatment which might be useful in subsequent elicitation of immunological action during immunosuppressive states. PMA and FMLP were used to artificially stimulate reactive ...
Tateishi Ken K National Institute of Agrobiological Sciences, 2-1-2 Kan-non dai, Tsukuba, Ibaraki, 305-8602, Japan, - - 2014
A new cell line, designated NIAS-SL64, was established from the fat body of the fifth instar larvae of the common cutworm Spodoptera litura. NIAS-SL64 cells grew as spindle-shaped and non-adherent cells in the insect-specific cell culture medium MGM-450 supplemented with 10% fetal bovine serum. Criterions for the establishment of the ...
Zhu Ha H The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, - - 2014
Decidualization of endometrium, which is characterized by endometrial stromal cell (ESC) decidualization, vascular reconstruction, immune cell recruitment, and plentiful molecule production, is a crucial step for uterus to become receptive for embryo. When implantation takes place, ESCs surround and directly interact with embryo. Decidualized stromal cells (DSCs) are of great ...
Cremasco Viviana V 1] Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts, USA. - - 2014
Fibroblastic reticular cells (FRCs) are known to inhabit T cell-rich areas of lymphoid organs, where they function to facilitate interactions between T cells and dendritic cells. However, in vivo manipulation of FRCs has been limited by a dearth of genetic tools that target this lineage. Here, using a mouse model ...
Di Mitri Diletta D 1] Institute of Oncology Research (IOR), Oncology Institute of Southern Switzerland, Bellinzona CH6500, Switzerland - - 2014
Aberrant activation of oncogenes or loss of tumour suppressor genes opposes malignant transformation by triggering a stable arrest in cell growth, which is termed cellular senescence. This process is finely tuned by both cell-autonomous and non-cell-autonomous mechanisms that regulate the entry of tumour cells to senescence. Whether tumour-infiltrating immune cells ...
Takahashi Toshio T Suntory Foundation for Life Sciences, Bioorganic Research Institute, Osaka, 618-8503, - - 2014
Non-neuronal acetylcholine (non-neuronal ACh) is predicted to function as a local cell signaling molecule. However, the physiological significance of the synthesis of non-neuronal ACh in the intestine remains unclear. Here, experiments using crypt-villus organoids that lack nerve and immune cells in culture led us to suggest that endogenous ACh is ...
Hassan Burhan B Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, - - 2014
We tested the antitumor efficacy of mTOR catalytic site inhibitor MLN0128 in models with intrinsic or acquired rapamycin-resistance. Cell lines that were intrinsically rapamycin-resistant as well as those that were intrinsically rapamycin-sensitive were sensitive to MLN0128 in vitro. MLN0128 inhibited both mTORC1 and mTORC2 signaling, with more robust inhibition of ...
Gerriets Valerie A VA Division of Pediatric Endocrinology and Diabetes, Duke University Medical Center , Durham, NC , - - 2014
Nutritional status is critically important for immune cell function. While obesity is characterized by inflammation that promotes metabolic syndrome including cardiovascular disease and insulin resistance, malnutrition can result in immune cell defects and increased risk of mortality from infectious diseases. T cells play an important role in the immune adaptation ...
Rougeot Julien J Institute of Biology, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The - - 2014
The zebrafish (Danio rerio) is increasingly used as a model for studying infectious diseases. This nonmammalian vertebrate host, which is transparent at the early life stages, is especially attractive for live imaging of interactions between pathogens and host cells. A number of useful fluorescent reporter lines have recently been developed ...
Rodríguez-Sanz Héctor - - 2014
In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively. Despite its high potential in tree breeding strategies, due to their ...
Zarrouk Marouan M Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee, Scotland, United - - 2014
The anti-diabetic drug metformin regulates T-cell responses to immune activation and is proposed to function by regulating the energy-stress-sensing adenosine-monophosphate-activated protein kinase (AMPK). However, the molecular details of how metformin controls T cell immune responses have not been studied nor is there any direct evidence that metformin acts on T ...
da Silva Priscila C A PC Departamento de Física, Universidade Federal de Viçosa, Viçosa, Minas Gerais, - - 2014
In multicellular organisms, cell motility is central in all morphogenetic processes, tissue maintenance, wound healing and immune surveillance. Hence, failures in its regulation potentiates numerous diseases. Here, cell migration assays on plastic 2D surfaces were performed using normal (Melan A) and tumoral (B16F10) murine melanocytes in random motility conditions. The ...
Rydell-Törmänen Kristina K Dept. Experimental Medical Science, Lund University, Lund, - - 2014
The ECM exerts great effects on cells, and changed composition may therefore have profound impact. Small leucine-rich proteoglycans, e.g. fibromodulin, are essential in collagen assembly. Our aim was to investigate the role of fibromodulin in healthy and fibrotic lung parenchyma, theorizing that fibromodulin-deficient animals would be protected against fibrosis. Repeated ...
Ekino Shigeo S Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan. Electronic address: - - 2014
The bursa of Fabricius is a primary lymphoid organ for B-cell development and gut-associated lymphoid tissue. After hatching, IgG-containing cells with reticular branches are found in the medulla of bursal follicles on frozen sections stained with anti-Cγ antibody, and IgM(+)IgG(+) B cells are detected in single-cell suspension of the bursa. ...
Zhao Shuang S MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, - - 2014
Brazilein, a natural small molecule, shows a variety of pharmacological activities, especially on nervous system and immune system. As a potential multifunctional drug, we studied the distribution and the transport behavior and metabolic behavior of brazilein in vivo and in vitro. Brazilein was found to be able to distribute in ...
Agliari Elena E Dipartimento di Fisica, Sapienza Università di - - 2014
Scope of the present work is to infer the migratory ability of leukocytes by stochastic processes in order to distinguish the spontaneous organization of immune cells against an insult (namely cancer). For this purpose, spleen cells from immunodeficient mice, selectively lacking the transcription factor IRF-8 (IRF-8 knockout; IRF-8 KO), or ...
Singla Arvind K AK Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Chandigarh, - - 2014
Caerulomycin A (CaeA) is a known antifungal and antibiotic agent. Further, CaeA is reported to induce the expansion of regulatory T cell and prolongs the survival of skin allografts in mouse model of transplantation. In the current study, CaeA was purified and characterized from a novel species of actinomycetes, Actinoalloteichus ...
Steers Nicholas J NJ United States Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, - - 2014
Proteasomes play an important role in cell homeostasis and in orchestrating the immune response by systematically degrading foreign proteins and misfolded or damaged host cell proteins. We describe a protocol to purify functionally active proteasomes from human CD4(+) T cells and dendritic cells derived from peripheral blood mononuclear cells. The ...
Jain Reema R Molecular Genetics of Cancer Division and Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, - - 2014
The epithelial cells of the thymus govern the differentiation of hematopoietic precursors into T cells, which are critical for acquired immunity. Thymic epithelial cells (TEC) provide molecular cues that direct precursor recruitment, commitment to the T cell lineage, thymocyte proliferation, and the processes of positive and negative selection. Despite the ...
Makino Yoshinori Y Laboratory of Pathology and Development, Institute of Molecular and Cellular Biosciences, The University of Tokyo Tokyo, Japan ; Career-Path Promotion Unit for Young Life Scientists, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University Kyoto, - - 2014
In vivo fluorescent imaging technique is a strong tool to visualize the various cellular events such as the proliferation, differentiation, migration, and a lineage tracing in living cells requiring no further experimental procedure such as immunostaining. During spermatogenesis, unique and dynamic histone exchanges occur. Since the timing and types of ...
Zhang Bin B Exosome and Secreted Nano-vesicle Group, ASTAR Institute of Medical Biology , - - 2014
Extracellular vesicle or EV is a term that encompasses all classes of secreted lipid membrane vesicles. Despite being scientific novelties, EVs are gaining importance as a mediator of important physiological and pathological intercellular activities possibly through the transfer of their cargo of protein and RNA between cells. In particular, exosomes, ...
Mori Yuki Y 1] Biofunctional Imaging, WPI Immunology Frontier Research Center (WPI IFReC), Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan [2] Center for Information and Neural Networks (CiNet), National Institute of Information and Communications Technology (NICT) and Osaka University, 1-4 Yamadaoka, Suita, Osaka 565-0871, - - 2014
Recent studies have demonstrated that immune cells play an important role in the pathogenesis of many neurological conditions. Immune cells constantly survey the brain microvasculature for irregularities in levels of factors that signal homeostasis. Immune responses are initiated when necessary, resulting in mobilisation of the microglial cells resident in the ...
Asano Krisana K Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, - - 2014
Toxic shock syndrome toxin-1 (TSST-1), a superantigen produced from Staphylococcus aureus, has been reported to bind directly to unknown receptor(s) and penetrate into non-immune cells but its function is unclear. In this study, we demonstrated that recombinant TSST-1 suppresses autophagosomal accumulation in the autophagic-induced HeLa 229 cells. This suppression is ...
Morgan Richard A RA NATIONAL INSTITUTES OF - - 2013
In this issue of Blood, Casucci et al present an elegant study that describes a potential new target for adoptive cell transfer (ACT), in this case CD44 splice variant 6 (CD44v6), and detail why it may be a good target for ACT and how to manage expected off-tumor/on-target toxicities.
Park Ki E KE University of Florida College of Medicine, 1600 SW Archer Rd. Gainesville, FL 32610, - - 2013
The cardiovascular cell therapy network was developed by the National Heart, Lung and Blood Institute to design and conduct clinical trials to advance the field of cardiovascular (CV) cell-based therapy. The Cardiovascular Cell Therapy Network successfully completed three clinical trials involving approximately 300 subjects across five centers and six satellites. ...
Huang Chenyu - - 2013
It has long been thought that the effectiveness and efficiency of physical therapy would improve if our understanding of the cell biology/biochemistry that participates in mechanics could be improved. Traditional physical therapy focuses primarily on rehabilitation, but recent developments in mechanobiology that illuminated the effects of physical forces on cells ...
Jacob M M Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, - - 2012
Tissue remodeling is critical during development and wound healing. It also characterizes a number of pathologic conditions, including chronic inflammation, fibrosis and cancer. It is well appreciated that reactive stromal cells play critical roles in these settings. However, understanding of the mechanisms involved in the differentiation of reactive stromal cells ...
Spence Heather J HJ The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, - - 2012
The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been ...
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