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Gagnon Kenneth B - - 2012
Cell motility is dependent on a coordinated reorganization of the cytoskeleton, membrane recycling, and focal adhesion to the extracellular matrix. Each of these cellular processes involves re-distribution of cell water, which is facilitated by the transport of inorganic ions (with obligatory water movement). Scratch-wound healing assays of Wistar C6 glioblastoma ...
Wang Xiaokun - - 2012
Ceramic-derived materials have shown enhanced osteogenic potential for bone tissue engineering applications. Silica is the major component of bioglass, and titania, the oxide complex of titanium, has been found to enhance osteoblast differentiation. In this study, three groups of sol-gel-derived silica-titania fibrous meshes with precursor ratios of Ti:Si = 7:3, ...
Ramaesh T - - 2012
Matrix metalloproteinase-9 (MMP-9) secreted by corneal epithelial cells has a role in the remodelling of extracellular matrix and migration of epithelial cells. Elevated levels of MMP-9 activity in the ocular surface may be involved in the pathogenesis of corneal diseases. N-acetylcysteine (NAC) has been used to treat corneal diseases, including ...
Bertseva E - - 2012
We present the microrheological study of the two close human epithelial cell lines: non-cancerous HCV29 and cancerous T24. The optical tweezers tracking was applied to extract the several seconds long trajectories of endogenous lipid granules at time step of 1μs. They were analyzed using a recently proposed equation for mean ...
Watson Alastair J M - - 2012
Although epithelial cells are continuously shed from the surface of the intestine, the intestinal epithelium maintains the integrity of the epithelial barrier. This is achieved through a highly dynamic process involving reorganization of tight junction and adherens junction proteins. This process both ejects the cell from the epithelial monolayer and ...
Favicchio Rosy - - 2012
Detection of multiple fluorophores in conditions of low signal represents a limiting factor for the application of in vivo optical imaging techniques in immunology where fluorescent labels report for different functional characteristics. A noninvasive in vivo Multi-Spectral Normalized Epifluorescence Laser scanning (M-SNELS) method was developed for the simultaneous and quantitative ...
Julio Gemma - - 2012
PURPOSE.: To establish the main indicator of progressive corneal exposure to dry eye conditions in an animal model. METHODS.: Cell surface covered by microvilli (SCM), intercellular junctions (IJs), cell area, cell shape, and cell shade were measured in 988 epithelial corneal cells from rabbit eyes that were kept open for ...
Paszek Matthew J - - 2012
Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton ...
Ahn Mee Young - - 2012
J Oral Pathol Med (2012) Background:  Pheophorbide a (Pa) is a chlorine-based photosensitizer derived from an ethnopharmacological herb, and our group recently synthesized Pa by the removal of a magnesium ion and a phytyl group from chlorophyll-a. In this study, the effect of photodynamic therapy (PDT) with synthesized Pa was ...
Sugiura Kana - - 2012
1) Fluorescence spectrum at 298 and 40 K and absorption spectrum at 298 K of each cyanobacterial cell was measured by the single-cell confocal laser spectroscopy to study differentiation of cell pigments. 2) Fluorescence spectra of vegetative ( veg) and heterocyst ( het) cells of a filamentous cyanobacterium Nostoc sp. ...
Takagi Mutsumi - - 2012
Time-lapse analysis of phase shift using phase-shifting laser microscopy (PLM) revealed that the laser phase shifts of primary normal human prostate epithelial cells (PRECs) and human prostatic carcinoma epithelial cell line (PC-3) cells during the mitotic phase were markedly higher than those in the interphase. The phase shift of PC-3 ...
Li Wen-Bo - - 2012
PURPOSE. To investigate the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into retinal pigment epithelium (RPE) cells, and identify development-regulating microRNAs (miRNAs).METHODS. RPE cells were derived from hPESCs. The expression of markers and miRNA expression profiles during differentiation were studied by immunocytochemistry, real-time reverse transcription PCR (RT-PCR), ...
Hovakimyan Marine - - 2012
Purpose: Trehalose has been shown to protect epithelial cells from desiccation damage in cell culture and the murine dry eye model. The present study evaluates the protective role of trehalose in reconstructed human corneal epithelium (3D-HCE) during desiccation. Material and methods: The morphology of 3D-HCE was examined using in vivo ...
Chan Leo L - - 2012
The ability to accurately measure cell viability is important for any cell-based research. Traditionally, viability measurements have been performed using trypan blue exclusion method on hemacytometer, which allowed researchers to visually distinguish viable from nonviable cells. However, the trypan blue method is often limited to only cell lines or primary ...
Daschner R - - 2012
We demonstrate the use of electrically contacted vapor cells to switch the transmission of a probe laser. The excitation scheme makes use of electromagnetically induced transparency involving a Rydberg state. The cell fabrication technique involves thin-film-based electric feedthroughs, which are well suited for scaling this concept to many addressable pixels ...
Bassett Erin A - - 2012
The vertebrate retina is a well-characterized and tractable model for studying neurogenesis. Retinal neurons and glia are generated in a conserved sequence from a pool of multipotent progenitor cells, and numerous cell fate determinants for the different classes of retinal cell types have been identified. Here, we summarize several recent ...
Ebenezer Vinitha - - 2012
Power plants employ chlorination for controlling biofouling in the cooling water system. Phytoplankton drawn into the cooling water system could be impacted by chemical stress induced by the oxidizing biocide. It is likely that microalgae, being sensitive to chlorine, could suffer damage to their cellular structure and function. In this ...
Mittal Anupama - - 2012
In the present study, porous PLGA microparticulate scaffolds (PMS_P), surface-hydrolysed scaffolds (PMS_Hyd) and cytomodulin-coupled scaffolds (PMS_CM) were prepared and characterized. After coupling the particles with cytomodulin, the size was reduced from 334 µm (span 0.53) to 278 µm due to hydrolysis, and contact angle also decreased from 70.87 ± 8.56 to 31.43 ± 7.43, indicating an ...
Lawrence Brian D - - 2012
Silk fibroin films are a promising class of biomaterials that have a number of advantages for use in ophthalmic applications due to their transparent nature, mechanical properties and minimal inflammatory response upon implantation. Freestanding silk films with parallel line and concentric ring topographies were generated for in vitro characterization of ...
Yoshiba Nagako - - 2012
Recent studies have employed two markers, alpha-smooth muscle actin (α-SMA) and STRO-1, to detect cells with mesenchymal stem cell properties in dental pulp. The present study aimed to explore the expression profile of α-SMA and STRO-1 in intact dental pulp as well as during wound healing in adult dental pulp ...
Feng Yun Y School of Pharmacy, University of Reading, United - - 2012
Corneal tissue engineering has improved dramatically over recent years. It is now possible to apply these technological advancements to the development of superior in vitro ocular surface models to reduce animal testing. We aim to show the effect different substrates can have on the viability of expanded corneal epithelial cells ...
Chen Y - - 2012
Purpose: To study the dose enhancement of golden nanoparticles on a voxellized cell phantom irradiated by proton beam. Methods: A Monte Carlo application was constructed with GEANT4 version 9.4 using various physics processes. A water cube of 5 mm × 5 mm × 5 mm consisting of a realistic human ...
Matthews Q - - 2012
Purpose: To use single-cell Raman spectroscopy (RS) and principal component analysis (PCA) for early treatment monitoring of radiation response. Methods: Two human tumour cell lines, one known to be radioresistant (H460, SF2 = 0.64) and one radiosensitive (LNCaP, SF2 = 0.27), were treated with daily 2 Gy fractions of 6 ...
Thorsrud Andreas - - 2012
To assess the hypothesis that during corneal crosslinking (CXL) treatment, riboflavin and ultraviolet-A (UVA) may have a toxic effect on human limbal epithelial cells. Center for Eye Research, Department of Ophthalmology, Oslo University Hospital Ullevål, Oslo, Norway. Experimental study. In this vitro study, limbal biopsies from corneoscleral rims collected after ...
Sweeney Sean T - - 2012
The selective removal of cells by ablation is a powerful tool in the study of eukaryotic developmental biology, providing much information about their origin, fate, or function in the developing organism. In Drosophila, three main methods have been used to ablate cells: chemical, genetic, and laser ablation. Each method has ...
Demidova Elizaveta V - - 2012
Studies of the impact of terahertz radiation on living objects present a significant interest since its use for security systems is currently considered promising. We studied the non-thermal impact of terahertz radiation on E. coli/pKatG-gfp biosensor cells. The Novosibirsk free electron laser (NovoFEL), which currently has the world's highest average ...
Ferreira Sílvia A - - 2012
Amphiphilic mannan, produced by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, self-assembles in aqueous medium through hydrophobic interactions among alkyl chains. Resultant nanogel is stable, spherical, polydisperse, with 50-140 nm mean hydrodynamic diameter depending on the polymer degree of substitution, and nearly neutral negative surface charge. No ...
Sweeney Sean T - - 2012
Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can ...
Liu Chin-Yi - - 2012
Solution-processed bulk heterojunction solar cells from silicon nanocrystals (Si NCs) and poly(3-hexylthiophene) (P3HT) have shown promising power conversion efficiencies. Here we report on an attempt to enhance the performance of Si NC-polymer hybrid solar cells by using poly[2-methoxy-5-(3',7'-dimethyloctyloxy)-1,4-phenylenevinylene] (MDMO-PPV) as a hole conductor, which is expected to yield a higher ...
Das Vallabh E - - 2012
To investigate whether neuronal activity within the supraoculomotor area (SOA-monosynaptically connected to medial rectus motoneurons and encode vergence angle) of strabismic monkeys was correlated with the angle of horizontal misalignment and therefore helps to define the state of strabismus. Single-cell neural activity was recorded from SOA neurons in two monkeys ...
Seifert Jessica R K - - 2012
Trans-epithelial migration describes the ability of migrating cells to cross epithelial tissues and occurs during development, infection, inflammation, immune surveillance, wound healing and cancer metastasis. Here we investigate Drosophila primordial germ cells (PGCs), which migrate through the endodermal epithelium. Through live imaging and genetic experimentation we demonstrate that PGCs take ...
Liu Jingbo J Weill Cornell Medical College, New York, New York, - - 2012
To evaluate a silk fibroin (SF) biomaterial as a substrate for corneal epithelial cell proliferation, differentiation, and stratification in vitro compared with denuded human amniotic membrane (AM). Primary human and rabbit corneal epithelial cells and immortalized human corneal limbal epithelial cells were cultured on the SF and denuded AM, respectively. ...
Gerhart Jacquelyn - - 2012
Murine and human skin were examined for the presence of Myo/Nog cells that were originally discovered in the chick embryo by their expression of MyoD mRNA, noggin and the G8 antigen. Myo/Nog cells are the primary source of noggin in telogen hair follicles. They are scarce within the interfollicular dermis ...
Shergill Bhupinder - - 2012
Notch signaling controls diverse cellular processes critical to development and disease. Cell surface ligands bind Notch on neighboring cells but require endocytosis to activate signaling. The role ligand endocytosis plays in Notch activation has not been established. Here we integrate optical tweezers with cell biological and biochemical methods to test ...
Schachtner Hannah H The Beatson Institute for Cancer Research, Garscube Estate, Bearsden, Glasgow, United - - 2012
We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex ...
Li Xin - - 2012
Abstract Objective: To investigate the effects of 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) on dermal fibroblasts from hypertrophic scars (HSs). Methods: HS samples were obtained from five patients who underwent surgery, and normal skin from healthy donors was used as a control. Dermal fibroblasts were isolated and cultured with various ...
Hauck Stefanie M - - 2012
Equine recurrent uveitis is a severe and frequently blinding disease in horses which presents with auto-reactive invading T-cells, resulting in the destruction of the inner eye. Infiltration of inflammatory cells into the retina and vitreous is driven by currently unknown guidance cues however surgical removal of the vitreous (vitrectomy) has ...
Kearns Victoria - - 2012
Subretinal transplantation of functioning retinal pigment epithelial (RPE) cells grown on a synthetic substrate is a potential treatment for age-related macular degeneration (AMD), a common cause of irreversible vision loss in developed countries. Plasma polymers give the opportunity to tailor the surface chemistry of the artificial substrate whilst maintaining the ...
Yang Lan - - 2012
Hypoxia-induced retinal ganglion cell (RGC) death has been proposed to be the critical event in the pathophysiology of glaucoma. Therefore, delaying or halting RGC degeneration, known as neuroprotection, is a novel and promising approach with potential clinical applications for treating glaucoma. In this study, we investigate hypoxia-induced cell death of ...
Li Jianghua - - 2012
The radiotherapy-related types of nasopharyngeal carcinoma (NPC) have been established, which give the most effective treatment for NPC patients using the individual therapy. To diagnose the types of NPC, we assess the general NPC cell lines CNE1, CNE2 and normal nasopharyngeal cell line NP69 using optical coherence tomography (OCT) in ...
Young Steven H - - 2012
We have examined the role of protein kinase D1 (PKD1) signaling in intestinal epithelial cell migration. Wounding monolayer cultures of intestinal epithelial cell line IEC-18 or IEC-6 induced rapid PKD1 activation in the cells immediately adjacent to the wound edge, as judged by immunofluorescence microscopy with an antibody that detects ...
Yan Lu - - 2012
As graphene becomes one of the most exciting candidate for multifunctional biomedical applications, contact between eyes and graphene based materials is inevitable. On the other hand, eyes, as a special organ in human body, have unique advantages to be used for testing new biomedical research and development, such as drug ...
Prasov Lev - - 2012
The basic-helix-loop helix factor Math5 (Atoh7) is required for retinal ganglion cell (RGC) development. However, only 10% of Math5-expressing cells adopt the RGC fate, and most become photoreceptors. In principle, Math5 may actively bias progenitors towards RGC fate or passively confer competence to respond to instructive factors. To distinguish these ...
Lu Michael - - 2012
We investigated the role that the cystic fibrosis transmembrane conductance regulator (CFTR) may play in Cl(-) transport in the acinar and ductal epithelial cells of rabbit lacrimal gland (LG). Primary cultured LG acinar cells were processed for whole-cell patch-clamp electrophysiological recording of Cl(-) currents by using perfusion media with high ...
Chandhoke Surjit K - - 2012
Polymorphisms in the gene encoding the heavy chain of myosin IXb (Myo9b) have been linked to several forms of inflammatory bowel disease (IBD). Given that Myo9b contains a RhoGTPase-activating protein domain within its tail, it may play key roles in Rho-mediated actin cytoskeletal modifications critical to intestinal barrier function. In ...
Migliario Mario - - 2012
This in vitro experimental study has been designed to assess the effects of photodynamic antimicrobial chemotherapy (PACT) on human keratinocytes proliferation. Human keratinocytes (HaCaT) monolayers (∼0.5 cm(2)) have been irradiated with 635 nm red laser light with a fluence of 82.5 or 112.5 J/cm(2) in the absence or presence of toluidine (TB). Cell ...
Bayram Hilal - - 2012
A limited number of clinical studies indicate the supportive role of low level laser therapy (LLLT) on medical and/or surgical approaches carried out in treatment modalities for bisphosphonate related necrosis of jaws (BRONJ), the most common side effect of bisphosphonates used to inhibit bone resorption. The purpose of this study ...
Wei Wei - - 2012
Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells. The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply ...
Doughty Michael J - - 2012
Goblet cells of the conjunctiva are the main source of mucus for the ocular surface. The objectives of this review are to consider the goblet cells as assessed by various histological, cytological and electron microscopy methods, and to assess the consistency of published reports (over more than 25 years) of ...
Chen Zhenzhen - - 2012
Determination of intracellular bioactive species will afford beneficial information related to cell metabolism, signal transduction, cell function and disease treatment. In this study, the first application of microchip electrophoresis-laser induced fluorescence (MCE-LIF) method for concurrent determination of reactive oxygen species (ROS) and reactive nitrogen species (RNS), i.e., superoxide (O2-•) and ...
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